TTHERM_00363140 cDNA ORF clone, Tetrahymena thermophila SB210

Gene
Clones
gRNAs

Summary

Gene Symbol	TTHERM_00363140
Entrez Gene ID	7842393
Full Name	transmembrane protein, putative
Gene Type	protein-coding
Organism	Tetrahymena thermophila SB210

mRNA and Protein(s)

mRNA	Protein	Name
XM_001007425.2	XP_001007425.2	transmembrane protein, putative

Pathways from BioSystems
Gene Ontology

The following TTHERM_00363140 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TTHERM_00363140 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTe37745	XM_001007425.2 Latest version!	Tetrahymena thermophila SB210 transmembrane protein, putative partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TTHERM_00363140 ( XM_001007425.2 ) cDNA ORF clone, Tetrahymena thermophila SB210 -> XP_001007425.2 Tetrahymena thermophila SB210 transmembrane protein, putative partial mRNA.

Price & Availability↑

CloneID	OTe37745
Clone ID Related Accession (Same CDS sequence)	XM_001007425.2
Accession Version	XM_001007425.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7845bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1433692800000
Organism	Tetrahymena thermophila SB210
Product	transmembrane protein, putative
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_002476569). On Jun 9, 2015 this sequence version replaced XM_001007425.1. COMPLETENESS: incomplete on both ends.

ATGAAGTACT TATTGCTAAG TTTAATCTCT ATCTTAAATA TTATATTTGG ATAACAATGT 
CCTCTTTTGC AAGTATACCA AAATCCTTAG AATATTTAAG GTTTTTCTAT AAAAAACTTT 
CTTAGAATAC CATAAACGAA TTTACTCTTA ATTAATACAG TATTTTCTGG AGGTAATGAT 
TCAAGTATAG TTTACTACAA TGATATGTCA TCCATTTCTG GAGAGATCAT CAATGTTATT 
AAGACTGACT ATTACATATT AGACTTTTAA TATAACTCTG CAACTGACTA GATTGTAGTT 
GCAAATTCTG GTAATCTTAT CTTTGCAGAT CCTTATACTC TAAATGTTAT TAGTTCTTTT 
TCTACTCCCT AGTTGTAGTC AATTCAGATT ATAAAAAATA CAAACTTCAT TATCCTTACT 
AAAGGTTATA ATAAACTAAA TATATTTGAC TTCGTAAAGT AATAAATAGT TTAAGTTATG 
GATAACACAA ATTAACTTCA AACCTTTCCA GATAATTCTA GTCTTGTACA ATACTATTCC 
AATATATATG CACTTAGCAG TGGTGATAAT ATTATATTAA CTACAAATGA TATGGGTGTA 
ATTTCTTGGG GAATAGATCT ATAATTGCTT AGCTATTAAT TTTATGGGTA TATTCCAGAT 
TCATAAGTTG TCTAAAAAAA TGATGCGAAA AGATGTTTCA CAAAACATCC AACTCAAGAT 
ATCATATTTA TTGCTGGAAA ATCATTAGAG ATTATAGCAG TAAAAATAAT TGACATCAAA 
AAGGGACTTT ACAAAACTCT TTTAAAAATG TCATTGAATG GGTATAAGAC AACTGATGCA 
ATTTTAAGCG TAAAATTTGT TTACTATAAC AAAAATGGTT ATACATATCC CACTATTTGG 
TTAGGAGTAC AAAATATTGC ATACTATGCT TATTTGCAGT TTAATAGTGA TTTTTCTACT 
ATCTAACTTT AACTTGGTAG CTCAATAGCA ATTTAAAGTT AAGCAATATG GAATTAAATA 
GAAGAAAGCA CAATCTTCTT AATATCTTCC AGTTTTTATG TGAGTATATT TAACTACTAA 
TCATTTTAGT TAACGTACAA TCTATATTTT TTTGCGGGTT ATTTTAGTAG GAGATTTATT 
AGACAATAGT CAGGCTCAAC TGATAGATTT GTTTTTCTTT AAACAAATAA AATATTACTT 
TATGAAAGAG GTAACTTTGG AACTTATGAT CCTAGTACAT AAAAATCTAC ATTAATTTAC 
AGCGGGTTTT ATAATTATGG AAGCTTTTAT TAAATAAAGA ATATTTTTGA TTGGTATTTT 
CTTAAAAGTG TCAATTCAAA TGGATAAAGC TTAATCTAAA TATTTCCTAT ATATCCTCTT 
AGTCAAACAG CAAATGTTAC TAACATTACA TCTTCTTTTG GATTAAGTTG GTCAAATGTT 
AATTAAAATT TAGATCCTTA TTATTTAAAT GGAAAATTTT GGGTAGTATT AGCTTTTCCT 
GCTAAAGCCA ATACAGAAAA CTATCTTTTC TAACTGATTA ATTGCTAGTC TACAACAGAG 
AGATATAATC TAACTTCTAA TTAATCTGAT GTTCGATAAA TTTAAACAGC GTTTGGAATT 
GCCTCCTTAG AGAATACTAA TAATTTAGAA TTAATAGGAG TAGATAATTA AGGCACAATC 
TATTCTTGGG ATTTAAGTAT GCAAACAATA CCTTTTAAAA ACTCTATAAA ATTCAGTATA 
TGCAAGAATT CTTAAATTGG AGATATTTTT TACTATGGAA ATTCTAAATA TTTGATAATG 
ACTTGTGATG ATAATAATGT TTATTCATTT AATCTTTAAA CAGGAAATTA TCAAAAGTTA 
TTAACTTTAA GTAGCTAACC TTTGGCTTTG AAAGCTTTTT CAAAACCATA ATTAGTAGCA 
ATAGGGGATT CTAATTAAGG AATTGCTTTC ATCTACAAAT TTAACACAAT TTCTCTTTAT 
TTTGATTACT TTTTGCAAAT ACAATAAAAA TAAATTTATG ATAAACTTAC TTACATAGAT 
ATGCTCAACG ATTATACAAT TTGGGTTTAA TATGGTTAGG GAAATATTTT CTACTCAATC 
TAAGATTGCT TATCTGATTC TAGTCTCTGT ACTTAATGTA CTTAAAAGTA TTATTTTAAT 
GCCACTAATT AATATGATAT AAATGGACTA TATGGTGCAG GTTCATAAAG TTAACCATTT 
ACAACTTCTA GAAGTTTTCT TACTGCTATG ATGAAAGCTC AATATTACCA ATAAACAATA 
AGTGGAGTGT AAAATATGGA TGTAAACTTT TCTATTGAAC CAGGTTACTT TTTGAACTTA 
AATCCAAATC TGATGAATTT CTATTTCAAC AAAATTATCT CATTGACATT TTAGAGTAGC 
TAACCTGGAA TTTATGCAAG TTTAAAATAC CAAAACACCT TGAGTTTGTA GAACTATCAC 
TAAATCAAAT TGCAAGATAT TGCAATATAT TATGGATTGG ACACTATATC AGCAAATTGT 
GGATTGATGT TTTAAAATAT ATAACAAGGA GTAGTCATTA ATAATATCTA GTAATTCAAT 
CTCAATTCAA CATCTTTAAC AAAGAGTTGT TAATAAATAT TAATTTAATA AACCTAATTG 
CAAGTTTAGA AATATGAAAT AGTATAAGAA GATTTCACAA ATCACTAATT CGTTATCAGC 
ACTTTTAATT CTACTTAGAT TGAATTATTT AACATTTCTA TAGTTAATTC TACTTTTGGT 
TAAACATTTA GCCTTTTGTA GTAGTAATCT AATATTCAAG CTAAGATAGC TAATCTTACT 
ATATCAAATA ATATATGTTC TATTAACAAT AACTAAGTAG AAGAAGATAC TATATCTGTT 
CTTTTCTCAG CTGGATTATA TATAGTTGAT GGTGTGCTTA TAAGCAATAA TTAATTTTGC 
AAGAAATCAA TTTTTTCAAC TGTTACAACT CTAACTCAAC TCAATCAAAT TTTCTCATTT 
AGCAATATAT TAGTTTAAAA TAATACATTT TAAGCAAGAA CTACTTACAT ATTTTTTAAT 
GCATTTTATT CAATCAGATA ATCCCCTTCT CACGAACTGA TTTTAAATAA TGTTTAGTTT 
ATAAATAACT AGTTATCTTA GCAAAGCGCA TAAGATTTAA ATATTGCCTA ATACTTTTAA 
ACTAATAAAA TTGCCAATTT TTAGATATAA AATACCTCCT TAATTGATCA TTTTGACATT 
TAGCTTGGGT TAATAGAGAT AGCAAACTAA GTCTTTATCG ATGGATTTTC TTGTGTAAAT 
AATGATAATT ATATTAATCA AATACCTAAC AAAACTACAG CAGGTTGCTT ACAAATCAAA 
GAAGTATAGT CAGCTAATTT TACCTAAATT TAAATTATTA ACAAAAAAGC TCAAGATACA 
AACCTGATAT CAATATTAAA TAGCGATATT TAATAAGCAA ATGTTACCAT AACTGATGGT 
TTTTTTAGTG ATTTATATTT ATATTAAAAT TGTGTAAATA CTTAAGCTAT ACCTTTGTAG 
ATAGTTAGTG GTTATTAAAT CTAGGTCGAA ATAGACAATT GTATATTTTA GAATATATTT 
TTGAAGAGTT TAGATTACAC ATTAACCTTC TCAAGCACAG CATTATATAT TTTAAATTAT 
GTTGGTTCAA TAATTATTAC AAATTCTTAA TTTTTGGATT CCTATAGTAA CTCCTTATAT 
GGTTTTGCCT ATATTTAGAC TAATGAATTA ATTATAGAGA ATGTCACTTT TAATAATTCT 
ACTTTTACAG ATGATTAGTC TTTGTCTTTA TTTAACTCTT AAGGAAGTAT GATAAATGCT 
AAGGCTCATA ATATAACTAT TTTGAATAGT AATTTCTCTA AAACTACAGC ATCTAAAGGT 
GGTTTTCTTT ATTTAACGAG TTTTGGTGAT CAATTAAACA TTAATTTTAC TAATACTCTA 
TTTAGTGAAG GATATTCTTC AATCGATGGT AGTGCTATTT TCATAGATGA TGGAGGTTAG 
ATTCTTAATC TAAATTGTGA CAATTGTCAA TTCAGTAATT TGTATACTCT AATGGATAGT 
GCTTCAACTC TAGCATAACA AAAATATACT AAGAGATAAT CTGATAAGAG AAATAATATT 
TAATTTTAAG GAGGATATAT TAAAAATGTG AAGGGAGTTT CAGATAGCTA CTTCATTGAC 
GTAGTTAACA CTGATATATT ATTTTAGAAC ATAAAATAAA TTTTATCAGA GGAATTCAGC 
TCAAACCAAA TATCTTTTAG TTAATACATT AATAAAACAG GTGTTTCTTA GCAATCTACA 
CTAACTAATT TATAAAATTC AAATTTAACT ATTGATAAAT GTAATTTTAC AAACCTTTAA 
ATTGAATCTC CATCAGCAAC TTTTCCTTTA TTAATCAGGT CTTAAAATTC TTACATAAAA 
ATAAGTAATA CACACTTGCT AAATTCTTCA TTTACAACAA GTGTAATTTA TTCAATCTAA 
AGCTAATTAG AGCTTTCTTA GATAATATTT AAAAACATTA GCTAAAAACT TTTTTTAAAA 
AGATTAATTT AACAAAATAT TTATTAAACA CCTTAAATAT CCTCTTCTTC TTTGATTATA 
GCATCAGAAT CAATATTAAA TATAAGCTAA GGTAGCCTTT TCTCTAATAT TATATGCAAT 
TCTAACTGTA ATGGTGGAGC TATTTAAGTT TCAAAAGGTA CATTTAATAT TGATAGCACA 
GTTTTCTAAG AAATTCAATC TACATTTGGA GGCGCGTTAT TCATTCAAGG AATTAATGAT 
ACCAACAAAA TATCAAATAC TTAATTTTAA AATTGCTTTT CTTAAAATGA TGGAGGAGTA 
TTATATCTAA ATGCACTTTA AAATGATAAA TTTTCTATGA TCATAGACCA AACAACCTTT 
ATTGAAAATT CTTGCAATAC CAGAGGAGGT TCTCTGTATG TTGATTCTTA AATATTAAAT 
TCTGCTTATT AAAGTATCAA AATGAAGAAT TCTAAAATAA TTAACAACAA AGCTAGTATT 
GGAGGAGGTA TTTATCAATA AAATTTATCT GTAGACACTT AAGAAAGCAA TATAATCACT 
AATAATACTA TTTTGATTTA TGGAAAAGAT GATATTTCAT ATCCTTCACA GTTGAGAGTA 
AAGAATATTG ATGAATTTAT ATAGCTTAAT AATGCGGTAG TATAAAAACA CTAAATAGTA 
ATAAACAATT TTAGGAGCGG AGCTAATATA TCTGAGATTT AATTTGTGTT ACTGAATAAT 
AAAAATGAAT TAATTTATCC AATCACTTCA GATGATTACA ATACATACTA AGTTAATGTT 
AATTTTGATC CAGAAACCAA AGATTTATCG TCATATTCTC TAAGTGGAGA TCTTTTTGCA 
AAATATGATG AACATTTGCA AGCCTTTAAA TTTAGTAATA TAAATATCAT AGGTACTCCA 
GGTACATCAG CTAACATATC TTTTACTTCT AAATAGATCT ACATATTAGA TTAAAAAACA 
CAAAAATTCA TCTAAAATTA CACTTTTAAT ATTCTTATTA ATTTCAGACT ATGTGGTCCT 
GGAGAAAAAA TCAATAATCT AAATTAAGTA ACTGAGTGTT AAATATGTCC TTAAAATTAT 
TACTCTTTTG ATGTTTCTAA TTGCTTAGAA TGTCCTTAGG GAGCTACATG CTATGGAGGA 
ACAAATATAG TAGCTAATTC TGGATATTGG AGAAAAAGTA ATACAAGCAA TTTATTAATT 
GATTGTTCTA ATCTATCAGA AAATTGTGTA GGTGGTAGCT TTGGAAATCA TATTTGTTAT 
GAAGGTCATA TTGGAGCTCT TTGTGAAGAA TGTGATATTT TTGGTGAGTA TTGGGGGTAA 
TCTTATGCAA AGTCAGCAAA ATATTCATGC ACAAGGTGTG ATTAGATTAA AGGAAATGTT 
TGGGTTTTAA TATTAGTAAC AGTTTGGACT ATACTTTCAA TGTGCTTAGC AATTAAAGGA 
GATGTTGATG TTCTTAGAAA AAATGTAGCA ATTTTGGCAA TACAAAAAAA TATTCTATTA 
AGAAAAAAAT CTTTGAGCAT GAGAGAAAAG AGACATACAC TTTCAACTTA ATAAAATATA 
TTTTCTAAAA TTTCATAATA AAGCAAATAT TTTGCATCTT CTTAGCATTC ACCTTCCATA 
AAAAATAATT CAATACAATT TAAAAAACAT AAAGATAATT CAGGAATATT AATTAAAATG 
TTTACTAACT ACATACAAAT CGTAGGATCG ATTGCAACTT TTAACCTTTC AATTCCTTCT 
GGAATTTTTG AGTTTCCTTA AACAATTGGA CAGCCTCTTA ATAAAACTAT GAGTTCTCTT 
GATTGTGCTT TAAAGGATTT AAACTCAAGC TAATAGATAA GATTTCCTTG GTATATTTTA 
ACAACTGCAA TTATGTTTTT AATAATTTAT ATTTAGCCTG ATTTAGTTGC CTAAATAATT 
GCTCTGCTCT CGTGTAGATA AGTAGGTGAT ACAAATTATA TACTATCTAA TGTTTCCTTT 
GTTTGCTATT CCAGAGAACA TTAATTCTAC ATTTTGGCTC TTATTATTCC TATGCTACTA 
CTTTGGGTTT TTATAATTCC TGCTATATTT TTTTTCCTGT TAAGGAGAAA CAAAGATAAA 
TTAGAATCTA GCGAAATTAA GTTGAAATAT GGATTTTTAT ATAAAGAATA TTAAAATTAT 
GCATTTTATT GGGAATTTAT CAAGATGGCT GAGAAATTAG CAATTATTCT TGTCTTGAAT 
TTCTATTCTC AAAGTATTGT TACAAAAGGA ATACTTGTTT TTATCATTAT TGCTGCCTAT 
GGTATTCTTA CTTTGATAAT CCATCCCTAC TAAGAGACAG AAATAAATGA AATAGATTTT 
AATTCAACAA ATGTTTGTGC TTTGACTGTT CTTTTTGGTT TATTTATGTA TGATAACCCT 
TTCTTTTATT TCGTCTATAC CTCATTTGGA ATTATTATAA TTATGAATTC ATGGTTTATT 
TGTAAGATAT TAAGCAAAAT CATCAAAGGC TATATCCCGA AAATTAAGGC AGTAATACAC 
AGAGCAGTGG AATCACTGTC AAAAAGAATA TCTTATTTTA AAAGATTTGT TAAAAAAAAT 
GAAAAAGAAT AAATTAAACC TGAAATAAAA TAGAAATTCT AGACAATTTT TAAGAAGATT 
TTATCTTTAA AACCATAAGA AAAGAAAAAA ATTTTCTTGG ATGCTATTTA AAAATAATTA 
TGTGAATCAT ATAGACATAT TTTTGAAGAG TAAAACAGCA ACTAGAAAAC TAATGAGTTG 
TAAATTAATG AACAATAAAA AATCTAGTCA GAAGATTTAT AAATATAAAA TGAAAACTCT 
TTTAAAAAGA GAGAAGAAAA TAAATAGGAT GAATAAAATG TCTCAATTGT AATAGATTCA 
CCTTTTTCTA AATATTAAGA TACAGTAGAA TAGCTAAAAT ATTATGATAA TAAGTTAACA 
TTTGAGACTG ATATTATGAA GATAGATACA AAAGAGGACT TCATATCTTT GCCTTAAGAC 
GCCAATTCCA GAAAGCTTAT CTATTATTAA TAAAAATAAA ACCTCATTTA AACTAAAAGC 
AAGTTTAAAA TCAAAAGTAC ACCTAGCTAA TAAGATAATT AAGTACATGA GCAAGATTAA 
AGTCCTCAAT CTTAAGACTC AGACCAAGGA TAAAACATAA GTTAAATTAT TTAAGGATAA 
ATAATTGATA AAATAGAAGA TAGTGATGAT ATTGATAAGG AATAAATAAA TCAAGAATTT 
TAAGAGGGAT AAAAGAATGT TACATATTTC TAAGATAAAA GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001007425.2
CDS	1..7845
Translation	MKYLLLSLISILNIIFGQCPLLQVYQNPNIGFSIKNFLRIPTNLLLINTVFSGGNDSSIVYYNDMSSISGEIINVIKTDYYILDFYNSATDIVVANSGNLIFADPYTLNVISSFSTPLSIQIIKNTNFIILTKGYNKLNIFDFVK*IVVMDNTNLQTFPDNSSLVQYYSNIYALSSGDNIILTTNDMGVISWGIDLLLSYFYGYIPDSVVKNDAKRCFTKHPTQDIIFIAGKSLEIIAVKIIDIKKGLYKTLLKMSLNGYKTTDAILSVKFVYYNKNGYTYPTIWLGVQNIAYYAYLQFNSDFSTILLGSSIAISAIWNIEESTIFLISSSFYVSIFNYSFLTYNLYFFAGYFSRRFIRQSGSTDRFVFLTNKILLYERGNFGTYDPSTKSTLIYSGFYNYGSFYIKNIFDWYFLKSVNSNGSLIIFPIYPLSQTANVTNITSSFGLSWSNVNNLDPYYLNGKFWVVLAFPAKANTENYLFLINCSTTERYNLTSNSDVRITAFGIASLENTNNLELIGVDNGTIYSWDLSMQTIPFKNSIKFSICKNSIGDIFYYGNSKYLIMTCDDNNVYSFNLTGNYQKLLTLSSPLALKAFSKPLVAIGDSNGIAFIYKFNTISLYFDYFLQIQKIYDKLTYIDMLNDYTIWVYGGNIFYSIDCLSDSSLCTCTKYYFNATNYDINGLYGAGSSPFTTSRSFLTAMMKAQYYQTISGVNMDVNFSIEPGYFLNLNPNLMNFYFNKIISLTFSSPGIYASLKYQNTLSLNYHIKLQDIAIYYGLDTISANCGLMFNIQGVVINNIFNLNSTSLTKSCILI*TLQVKYEIVEDFTNHFVISTFNSTIELFNISIVNSTFGTFSLL*SNIQAKIANLTISNNICSINNNVEEDTISVLFSAGLYIVDGVLISNNFCKKSIFSTVTTLTQLNQIFSFSNILVNNTFARTTYIFFNAFYSIRSPSHELILNNVFINNLSQSADLNIAYFTNKIANFINTSLIDHFDILGLIEIANVFIDGFSCVNNDNYINQIPNKTTAGCLQIKEVSANFTIIINKKAQDTNLISILNSDIANVTITDGFFSDLYLYNCVNTAIPLIVSGYIVEIDNCIFNIFLKSLDYTLTFSSTALYILNYVGSIIITNSFLDSYSNSLYGFAYITNELIIENVTFNNSTFTDDSLSLFNSGSMINAKAHNITILNSNFSKTTASKGGFLYLTSFGDQLNINFTNTLFSEGYSSIDGSAIFIDDGGILNLNCDNCQFSNLYTLMDSASTLAQKYTKRSDKRNNIFGGYIKNVKGVSDSYFIDVVNTDILFNIKILSEEFSSNQISFSYINKTGVSQSTLTNLNSNLTIDKCNFTNLIESPSATFPLLIRSNSYIKISNTHLLNSSFTTSVIYSISLELSIIFKNISKLFLKRLIQNIYTPISSSSLIIASESILNISGSLFSNIICNSNCNGGAIVSKGTFNIDSTVFEIQSTFGGALFIQGINDTNKISNTFNCFSNDGGVLYLNALNDKFSMIIDQTTFIENSCNTRGGSLYVDSILNSAYSIKMKNSKIINNKASIGGGIYQNLSVDTESNIITNNTILIYGKDDISYPSQLRVKNIDEFILNNAVVKHIVINNFRSGANISEIFVLLNNKNELIYPITSDDYNTYVNVNFDPETKDLSSYSLSGDLFAKYDEHLQAFKFSNINIIGTPGTSANISFTSKIYILDKTQKFINYTFNILINFRLCGPGEKINNLNVTECICPNYYSFDVSNCLECPGATCYGGTNIVANSGYWRKSNTSNLLIDCSNLSENCVGGSFGNHICYEGHIGALCEECDIFGEYWGSYAKSAKYSCTRCDIKGNVWVLILVTVWTILSMCLAIKGDVDVLRKNVAILAIQKNILLRKKSLSMREKRHTLSTNIFSKISSKYFASSHSPSIKNNSIQFKKHKDNSGILIKMFTNYIQIVGSIATFNLSIPSGIFEFPTIGQPLNKTMSSLDCALKDLNSSIRFPWYILTTAIMFLIIYIPDLVAIIALLSCRVGDTNYILSNVSFVCYSREHFYILALIIPMLLLWVFIIPAIFFFLLRRNKDKLESSEIKLKYGFLYKEYNYAFYWEFIKMAEKLAIILVLNFYSQSIVTKGILVFIIIAAYGILTLIIHPYETEINEIDFNSTNVCALTVLFGLFMYDNPFFYFVYTSFGIIIIMNSWFICKILSKIIKGYIPKIKAVIHRAVESLSKRISYFKRFVKKNEKEIKPEIKKFTIFKKILSLKPEKKKIFLDAIKLCESYRHIFEENSNKTNELINEQKISEDLINENSFKKREENKDENVSIVIDSPFSKYDTVELKYYDNKLTFETDIMKIDTKEDFISLPDANSRKLIYYKNLITKSKFKIKSTPSDNVHEQDSPQSDSDQGNISIIGIIDKIEDSDDIDKEINQEFEGKNVTYFDKS

Target ORF information:

RefSeq Version	XM_001007425.2
Organism	Tetrahymena thermophila SB210
Definition	Tetrahymena thermophila SB210 transmembrane protein, putative partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001007425.2

ORF Insert Sequence:

ATGAAGTACT TATTGCTAAG TTTAATCTCT ATCTTAAATA TTATATTTGG ATAACAATGT 
CCTCTTTTGC AAGTATACCA AAATCCTTAG AATATTTAAG GTTTTTCTAT AAAAAACTTT 
CTTAGAATAC CATAAACGAA TTTACTCTTA ATTAATACAG TATTTTCTGG AGGTAATGAT 
TCAAGTATAG TTTACTACAA TGATATGTCA TCCATTTCTG GAGAGATCAT CAATGTTATT 
AAGACTGACT ATTACATATT AGACTTTTAA TATAACTCTG CAACTGACTA GATTGTAGTT 
GCAAATTCTG GTAATCTTAT CTTTGCAGAT CCTTATACTC TAAATGTTAT TAGTTCTTTT 
TCTACTCCCT AGTTGTAGTC AATTCAGATT ATAAAAAATA CAAACTTCAT TATCCTTACT 
AAAGGTTATA ATAAACTAAA TATATTTGAC TTCGTAAAGT AATAAATAGT TTAAGTTATG 
GATAACACAA ATTAACTTCA AACCTTTCCA GATAATTCTA GTCTTGTACA ATACTATTCC 
AATATATATG CACTTAGCAG TGGTGATAAT ATTATATTAA CTACAAATGA TATGGGTGTA 
ATTTCTTGGG GAATAGATCT ATAATTGCTT AGCTATTAAT TTTATGGGTA TATTCCAGAT 
TCATAAGTTG TCTAAAAAAA TGATGCGAAA AGATGTTTCA CAAAACATCC AACTCAAGAT 
ATCATATTTA TTGCTGGAAA ATCATTAGAG ATTATAGCAG TAAAAATAAT TGACATCAAA 
AAGGGACTTT ACAAAACTCT TTTAAAAATG TCATTGAATG GGTATAAGAC AACTGATGCA 
ATTTTAAGCG TAAAATTTGT TTACTATAAC AAAAATGGTT ATACATATCC CACTATTTGG 
TTAGGAGTAC AAAATATTGC ATACTATGCT TATTTGCAGT TTAATAGTGA TTTTTCTACT 
ATCTAACTTT AACTTGGTAG CTCAATAGCA ATTTAAAGTT AAGCAATATG GAATTAAATA 
GAAGAAAGCA CAATCTTCTT AATATCTTCC AGTTTTTATG TGAGTATATT TAACTACTAA 
TCATTTTAGT TAACGTACAA TCTATATTTT TTTGCGGGTT ATTTTAGTAG GAGATTTATT 
AGACAATAGT CAGGCTCAAC TGATAGATTT GTTTTTCTTT AAACAAATAA AATATTACTT 
TATGAAAGAG GTAACTTTGG AACTTATGAT CCTAGTACAT AAAAATCTAC ATTAATTTAC 
AGCGGGTTTT ATAATTATGG AAGCTTTTAT TAAATAAAGA ATATTTTTGA TTGGTATTTT 
CTTAAAAGTG TCAATTCAAA TGGATAAAGC TTAATCTAAA TATTTCCTAT ATATCCTCTT 
AGTCAAACAG CAAATGTTAC TAACATTACA TCTTCTTTTG GATTAAGTTG GTCAAATGTT 
AATTAAAATT TAGATCCTTA TTATTTAAAT GGAAAATTTT GGGTAGTATT AGCTTTTCCT 
GCTAAAGCCA ATACAGAAAA CTATCTTTTC TAACTGATTA ATTGCTAGTC TACAACAGAG 
AGATATAATC TAACTTCTAA TTAATCTGAT GTTCGATAAA TTTAAACAGC GTTTGGAATT 
GCCTCCTTAG AGAATACTAA TAATTTAGAA TTAATAGGAG TAGATAATTA AGGCACAATC 
TATTCTTGGG ATTTAAGTAT GCAAACAATA CCTTTTAAAA ACTCTATAAA ATTCAGTATA 
TGCAAGAATT CTTAAATTGG AGATATTTTT TACTATGGAA ATTCTAAATA TTTGATAATG 
ACTTGTGATG ATAATAATGT TTATTCATTT AATCTTTAAA CAGGAAATTA TCAAAAGTTA 
TTAACTTTAA GTAGCTAACC TTTGGCTTTG AAAGCTTTTT CAAAACCATA ATTAGTAGCA 
ATAGGGGATT CTAATTAAGG AATTGCTTTC ATCTACAAAT TTAACACAAT TTCTCTTTAT 
TTTGATTACT TTTTGCAAAT ACAATAAAAA TAAATTTATG ATAAACTTAC TTACATAGAT 
ATGCTCAACG ATTATACAAT TTGGGTTTAA TATGGTTAGG GAAATATTTT CTACTCAATC 
TAAGATTGCT TATCTGATTC TAGTCTCTGT ACTTAATGTA CTTAAAAGTA TTATTTTAAT 
GCCACTAATT AATATGATAT AAATGGACTA TATGGTGCAG GTTCATAAAG TTAACCATTT 
ACAACTTCTA GAAGTTTTCT TACTGCTATG ATGAAAGCTC AATATTACCA ATAAACAATA 
AGTGGAGTGT AAAATATGGA TGTAAACTTT TCTATTGAAC CAGGTTACTT TTTGAACTTA 
AATCCAAATC TGATGAATTT CTATTTCAAC AAAATTATCT CATTGACATT TTAGAGTAGC 
TAACCTGGAA TTTATGCAAG TTTAAAATAC CAAAACACCT TGAGTTTGTA GAACTATCAC 
TAAATCAAAT TGCAAGATAT TGCAATATAT TATGGATTGG ACACTATATC AGCAAATTGT 
GGATTGATGT TTTAAAATAT ATAACAAGGA GTAGTCATTA ATAATATCTA GTAATTCAAT 
CTCAATTCAA CATCTTTAAC AAAGAGTTGT TAATAAATAT TAATTTAATA AACCTAATTG 
CAAGTTTAGA AATATGAAAT AGTATAAGAA GATTTCACAA ATCACTAATT CGTTATCAGC 
ACTTTTAATT CTACTTAGAT TGAATTATTT AACATTTCTA TAGTTAATTC TACTTTTGGT 
TAAACATTTA GCCTTTTGTA GTAGTAATCT AATATTCAAG CTAAGATAGC TAATCTTACT 
ATATCAAATA ATATATGTTC TATTAACAAT AACTAAGTAG AAGAAGATAC TATATCTGTT 
CTTTTCTCAG CTGGATTATA TATAGTTGAT GGTGTGCTTA TAAGCAATAA TTAATTTTGC 
AAGAAATCAA TTTTTTCAAC TGTTACAACT CTAACTCAAC TCAATCAAAT TTTCTCATTT 
AGCAATATAT TAGTTTAAAA TAATACATTT TAAGCAAGAA CTACTTACAT ATTTTTTAAT 
GCATTTTATT CAATCAGATA ATCCCCTTCT CACGAACTGA TTTTAAATAA TGTTTAGTTT 
ATAAATAACT AGTTATCTTA GCAAAGCGCA TAAGATTTAA ATATTGCCTA ATACTTTTAA 
ACTAATAAAA TTGCCAATTT TTAGATATAA AATACCTCCT TAATTGATCA TTTTGACATT 
TAGCTTGGGT TAATAGAGAT AGCAAACTAA GTCTTTATCG ATGGATTTTC TTGTGTAAAT 
AATGATAATT ATATTAATCA AATACCTAAC AAAACTACAG CAGGTTGCTT ACAAATCAAA 
GAAGTATAGT CAGCTAATTT TACCTAAATT TAAATTATTA ACAAAAAAGC TCAAGATACA 
AACCTGATAT CAATATTAAA TAGCGATATT TAATAAGCAA ATGTTACCAT AACTGATGGT 
TTTTTTAGTG ATTTATATTT ATATTAAAAT TGTGTAAATA CTTAAGCTAT ACCTTTGTAG 
ATAGTTAGTG GTTATTAAAT CTAGGTCGAA ATAGACAATT GTATATTTTA GAATATATTT 
TTGAAGAGTT TAGATTACAC ATTAACCTTC TCAAGCACAG CATTATATAT TTTAAATTAT 
GTTGGTTCAA TAATTATTAC AAATTCTTAA TTTTTGGATT CCTATAGTAA CTCCTTATAT 
GGTTTTGCCT ATATTTAGAC TAATGAATTA ATTATAGAGA ATGTCACTTT TAATAATTCT 
ACTTTTACAG ATGATTAGTC TTTGTCTTTA TTTAACTCTT AAGGAAGTAT GATAAATGCT 
AAGGCTCATA ATATAACTAT TTTGAATAGT AATTTCTCTA AAACTACAGC ATCTAAAGGT 
GGTTTTCTTT ATTTAACGAG TTTTGGTGAT CAATTAAACA TTAATTTTAC TAATACTCTA 
TTTAGTGAAG GATATTCTTC AATCGATGGT AGTGCTATTT TCATAGATGA TGGAGGTTAG 
ATTCTTAATC TAAATTGTGA CAATTGTCAA TTCAGTAATT TGTATACTCT AATGGATAGT 
GCTTCAACTC TAGCATAACA AAAATATACT AAGAGATAAT CTGATAAGAG AAATAATATT 
TAATTTTAAG GAGGATATAT TAAAAATGTG AAGGGAGTTT CAGATAGCTA CTTCATTGAC 
GTAGTTAACA CTGATATATT ATTTTAGAAC ATAAAATAAA TTTTATCAGA GGAATTCAGC 
TCAAACCAAA TATCTTTTAG TTAATACATT AATAAAACAG GTGTTTCTTA GCAATCTACA 
CTAACTAATT TATAAAATTC AAATTTAACT ATTGATAAAT GTAATTTTAC AAACCTTTAA 
ATTGAATCTC CATCAGCAAC TTTTCCTTTA TTAATCAGGT CTTAAAATTC TTACATAAAA 
ATAAGTAATA CACACTTGCT AAATTCTTCA TTTACAACAA GTGTAATTTA TTCAATCTAA 
AGCTAATTAG AGCTTTCTTA GATAATATTT AAAAACATTA GCTAAAAACT TTTTTTAAAA 
AGATTAATTT AACAAAATAT TTATTAAACA CCTTAAATAT CCTCTTCTTC TTTGATTATA 
GCATCAGAAT CAATATTAAA TATAAGCTAA GGTAGCCTTT TCTCTAATAT TATATGCAAT 
TCTAACTGTA ATGGTGGAGC TATTTAAGTT TCAAAAGGTA CATTTAATAT TGATAGCACA 
GTTTTCTAAG AAATTCAATC TACATTTGGA GGCGCGTTAT TCATTCAAGG AATTAATGAT 
ACCAACAAAA TATCAAATAC TTAATTTTAA AATTGCTTTT CTTAAAATGA TGGAGGAGTA 
TTATATCTAA ATGCACTTTA AAATGATAAA TTTTCTATGA TCATAGACCA AACAACCTTT 
ATTGAAAATT CTTGCAATAC CAGAGGAGGT TCTCTGTATG TTGATTCTTA AATATTAAAT 
TCTGCTTATT AAAGTATCAA AATGAAGAAT TCTAAAATAA TTAACAACAA AGCTAGTATT 
GGAGGAGGTA TTTATCAATA AAATTTATCT GTAGACACTT AAGAAAGCAA TATAATCACT 
AATAATACTA TTTTGATTTA TGGAAAAGAT GATATTTCAT ATCCTTCACA GTTGAGAGTA 
AAGAATATTG ATGAATTTAT ATAGCTTAAT AATGCGGTAG TATAAAAACA CTAAATAGTA 
ATAAACAATT TTAGGAGCGG AGCTAATATA TCTGAGATTT AATTTGTGTT ACTGAATAAT 
AAAAATGAAT TAATTTATCC AATCACTTCA GATGATTACA ATACATACTA AGTTAATGTT 
AATTTTGATC CAGAAACCAA AGATTTATCG TCATATTCTC TAAGTGGAGA TCTTTTTGCA 
AAATATGATG AACATTTGCA AGCCTTTAAA TTTAGTAATA TAAATATCAT AGGTACTCCA 
GGTACATCAG CTAACATATC TTTTACTTCT AAATAGATCT ACATATTAGA TTAAAAAACA 
CAAAAATTCA TCTAAAATTA CACTTTTAAT ATTCTTATTA ATTTCAGACT ATGTGGTCCT 
GGAGAAAAAA TCAATAATCT AAATTAAGTA ACTGAGTGTT AAATATGTCC TTAAAATTAT 
TACTCTTTTG ATGTTTCTAA TTGCTTAGAA TGTCCTTAGG GAGCTACATG CTATGGAGGA 
ACAAATATAG TAGCTAATTC TGGATATTGG AGAAAAAGTA ATACAAGCAA TTTATTAATT 
GATTGTTCTA ATCTATCAGA AAATTGTGTA GGTGGTAGCT TTGGAAATCA TATTTGTTAT 
GAAGGTCATA TTGGAGCTCT TTGTGAAGAA TGTGATATTT TTGGTGAGTA TTGGGGGTAA 
TCTTATGCAA AGTCAGCAAA ATATTCATGC ACAAGGTGTG ATTAGATTAA AGGAAATGTT 
TGGGTTTTAA TATTAGTAAC AGTTTGGACT ATACTTTCAA TGTGCTTAGC AATTAAAGGA 
GATGTTGATG TTCTTAGAAA AAATGTAGCA ATTTTGGCAA TACAAAAAAA TATTCTATTA 
AGAAAAAAAT CTTTGAGCAT GAGAGAAAAG AGACATACAC TTTCAACTTA ATAAAATATA 
TTTTCTAAAA TTTCATAATA AAGCAAATAT TTTGCATCTT CTTAGCATTC ACCTTCCATA 
AAAAATAATT CAATACAATT TAAAAAACAT AAAGATAATT CAGGAATATT AATTAAAATG 
TTTACTAACT ACATACAAAT CGTAGGATCG ATTGCAACTT TTAACCTTTC AATTCCTTCT 
GGAATTTTTG AGTTTCCTTA AACAATTGGA CAGCCTCTTA ATAAAACTAT GAGTTCTCTT 
GATTGTGCTT TAAAGGATTT AAACTCAAGC TAATAGATAA GATTTCCTTG GTATATTTTA 
ACAACTGCAA TTATGTTTTT AATAATTTAT ATTTAGCCTG ATTTAGTTGC CTAAATAATT 
GCTCTGCTCT CGTGTAGATA AGTAGGTGAT ACAAATTATA TACTATCTAA TGTTTCCTTT 
GTTTGCTATT CCAGAGAACA TTAATTCTAC ATTTTGGCTC TTATTATTCC TATGCTACTA 
CTTTGGGTTT TTATAATTCC TGCTATATTT TTTTTCCTGT TAAGGAGAAA CAAAGATAAA 
TTAGAATCTA GCGAAATTAA GTTGAAATAT GGATTTTTAT ATAAAGAATA TTAAAATTAT 
GCATTTTATT GGGAATTTAT CAAGATGGCT GAGAAATTAG CAATTATTCT TGTCTTGAAT 
TTCTATTCTC AAAGTATTGT TACAAAAGGA ATACTTGTTT TTATCATTAT TGCTGCCTAT 
GGTATTCTTA CTTTGATAAT CCATCCCTAC TAAGAGACAG AAATAAATGA AATAGATTTT 
AATTCAACAA ATGTTTGTGC TTTGACTGTT CTTTTTGGTT TATTTATGTA TGATAACCCT 
TTCTTTTATT TCGTCTATAC CTCATTTGGA ATTATTATAA TTATGAATTC ATGGTTTATT 
TGTAAGATAT TAAGCAAAAT CATCAAAGGC TATATCCCGA AAATTAAGGC AGTAATACAC 
AGAGCAGTGG AATCACTGTC AAAAAGAATA TCTTATTTTA AAAGATTTGT TAAAAAAAAT 
GAAAAAGAAT AAATTAAACC TGAAATAAAA TAGAAATTCT AGACAATTTT TAAGAAGATT 
TTATCTTTAA AACCATAAGA AAAGAAAAAA ATTTTCTTGG ATGCTATTTA AAAATAATTA 
TGTGAATCAT ATAGACATAT TTTTGAAGAG TAAAACAGCA ACTAGAAAAC TAATGAGTTG 
TAAATTAATG AACAATAAAA AATCTAGTCA GAAGATTTAT AAATATAAAA TGAAAACTCT 
TTTAAAAAGA GAGAAGAAAA TAAATAGGAT GAATAAAATG TCTCAATTGT AATAGATTCA 
CCTTTTTCTA AATATTAAGA TACAGTAGAA TAGCTAAAAT ATTATGATAA TAAGTTAACA 
TTTGAGACTG ATATTATGAA GATAGATACA AAAGAGGACT TCATATCTTT GCCTTAAGAC 
GCCAATTCCA GAAAGCTTAT CTATTATTAA TAAAAATAAA ACCTCATTTA AACTAAAAGC 
AAGTTTAAAA TCAAAAGTAC ACCTAGCTAA TAAGATAATT AAGTACATGA GCAAGATTAA 
AGTCCTCAAT CTTAAGACTC AGACCAAGGA TAAAACATAA GTTAAATTAT TTAAGGATAA 
ATAATTGATA AAATAGAAGA TAGTGATGAT ATTGATAAGG AATAAATAAA TCAAGAATTT 
TAAGAGGGAT AAAAGAATGT TACATATTTC TAAGATAAAA GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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TTHERM_00363140 cDNA ORF clone, Tetrahymena thermophila SB210

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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