TTHERM_00561350 cDNA ORF clone, Tetrahymena thermophila SB210

Gene
Clones
gRNAs

Summary

Gene Symbol	TTHERM_00561350
Entrez Gene ID	7834902
Full Name	transmembrane protein, putative
Gene Type	protein-coding
Organism	Tetrahymena thermophila SB210

mRNA and Protein(s)

mRNA	Protein	Name
XM_001010189.2	XP_001010189.2	transmembrane protein, putative

Pathways from BioSystems
Gene Ontology

The following TTHERM_00561350 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TTHERM_00561350 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTe40443	XM_001010189.2 Latest version!	Tetrahymena thermophila SB210 transmembrane protein, putative partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TTHERM_00561350 ( XM_001010189.2 ) cDNA ORF clone, Tetrahymena thermophila SB210 -> XP_001010189.2 Tetrahymena thermophila SB210 transmembrane protein, putative partial mRNA.

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CloneID	OTe40443
Clone ID Related Accession (Same CDS sequence)	XM_001010189.2
Accession Version	XM_001010189.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7728bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1433692800000
Organism	Tetrahymena thermophila SB210
Product	transmembrane protein, putative
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_002476522). On Jun 9, 2015 this sequence version replaced XM_001010189.1. COMPLETENESS: incomplete on both ends.

ATGAGAATGA AGAATTTAAA ATAGCATTTA GTACTGCTAT TTATTACAAT TATAAATTTA 
AAATAAATAT TTAGTTAAGA ATGTCCAAGT ATTGCAAGAT ATTTAAATAT CCTTCCTCAA 
TATTATGCAA GAAGCTTTTT GAAATTACCA AAAACCAACC TTTTAGTAGT TAATACAGTA 
TCTGATGTGA TTTAGGGATC TAGCGTAGTT TACTATATTG ACATTTCAAT GAACCCAGGA 
ATAGTTATTA CAACAATAAG GCCTGATTTT TTAATTACTT AAATGGAATA CATGCTCTCC 
CAAAACAGTA TATTGGTAGC TAACAACAAT AAAATTATCA TAGCTAATCC TTACACGCTT 
TAAGCTTAAA GTAATGCTGT ATTGAATTAG ATAGTAGACT TTTTTTAAGT ATAGAACACA 
AATTATATTG TTGTCACTCT AGCTTACAAT TAAATTGTTA TTTTAGACTC TTCAAGCTTA 
AAAACTATAA AGATATTGGA TAATACTAGC TTTCTAAGTA AAAAGGGTGT ATTATAAATA 
TCAACTCAAT TTTATACTCT TTCTTTTTAA CAGAATTTTA TAATTTGCTC TGATGAGTAA 
GGTTTGTATG CCTGGAAGGT CAATTTTGAG TAATCAGAGT TTAGTTACAA TGGATATATC 
CCTGATACTT TGAATTCCTC GATCAAAAAA TTTGAAAAAC ACCCAAAATA TGATATCTTA 
ATTGTAGGTG GATTAAAGAA TGTTAAATTC ATTTAAATAT AAGACCAAAA ATACACTACG 
TTATCAATAT AAACAGTTAA TTTTAGCAGT GTTGAAAGCA TTATATACTA GAGCATTAAT 
TCATCAGATA ATATAATTAT AGGAGATTCT CAGAGTATTT TCATTTACTA GTTTAGTGTA 
AACCCTGTTG ATAATACAAT AGAATCTTTT AAGTTGGATT ATTAAGTTTA ATAAGAAAAT 
CATTATGACG CCTACAAGCT TATTGAACTC CCAATTATTG TTTATGCAAG CAATAATTTA 
GTTACATTTT ATAATTACCA AAGCAAAAGT TTGAGCTATA ATTTAAACTT TTCTAGTGAT 
TACATAAATA AAAGCTTTAT TTTTTAGAAT TCTACTAAAT AGGAAGATTT ATTCTTAACT 
ATTAATCAGA ATAATTTAGT TGTGTATCAA AGAAGCAATT TAGGAGTTCA GTATTAGATG 
CAAAAATAGG CTTTGTAATC AGCTGTTCTT TAGTAGCAAA ATAGTTTTTA TAGAGTTAAA 
AACTGTGGAA ATTGCTTTTT CATCAAAACA AGTGATAGTT CTAATAAAAA TTATATTGTT 
TATACAAAAT TTGATGATAT TAAAAGTCCT TCATTCTAAT ACGATATTGG AACAGTTTCA 
TTAACTTGGA GTTAAATAAA CTAAAATTTA GATCCTTTTA TTTTAAATGA TTAACTATAT 
GTTGTTTTTT CTCAACCAAA CTAGATTACA CTTCCATAAG GAGAGAATTA TTACTTTTAT 
TTATTGCAGT TCTCGGCGGT AGATTTATCT TAAATTGCAG ATGTAAAGCT CACTACAAAT 
TAAAATTAAA ATAGAACCAG CTTAGCTGTT TATGATTCTT TGAATAAAAT TTTATATGGC 
ATAGACTCAG GAGGTACAGT CTATAAATAT GATATCTAAA ATAAAAACTT GGTTCAAAGC 
TTTTAAATTA ATGGCTGCTT AAATGCTTAT ATTGGAGAGA TTTTCATATA AGATCCTTAT 
TAATTCTTAA TTTTTGGATG TTAAAACAAT GATATTATAG CATATAACTT AGTAAGTAAA 
ACTTAAAGCA TTGTAACAAA TTTATACGTA TCTCCTTTTG TCCTGTAAGT ATTTAGTAGT 
TCCTCAATTA TTGCTCTTGG AGATATTGAT AGAGGATCTG CAAAAATTTT CAAGCTTAAT 
ACTATTTCCC AAAGTTTTGA TTTTTTTATA GAAATATTCT CTAGTAAGGG AGTGGACATC 
CTTCTATCTG TAGATTTATT AAGTGACTCT AATTTATTAC TTTAATTTAA AAATGGCATC 
ATATTCTTCC AATTTAGCTC TTGCTTGTAG GATATTAACA ATTGTTTGGG ATGCAACTAA 
AATTATTATT TCAAAATTCA AGATGGTCTT GATTCTAAAT AAAGCTATGG GCAAGGTAAA 
TAGGGTGTAG AATTTATTAC ATCTTAAAGC TTCCTCACAG CTGTTTTGAA GGCTCAAATA 
TACAAAAATC TAGTAAGATA ACTTACTTCT TTGGATGTCA ATATGTATAT CGATAAGGCT 
AATCCATTTA TTTTAAACTC CGGTTTACTC ATTTTTGACT TTAGCAATAT TATTTCTTTA 
TCTATGCAAT GCACAAGTTG TTAATAAAGT GAGTATTTTA GTTTATATTC ATCAGATTAG 
TTGCTATTAT AAAACTTTGT TCAATTTAAT TTGAATTAAG TTTCAGTGAA CTTTACAAAT 
TTAAGTTATA GTCAGAGCTG TGGCCTAAAA ATTTAAAATA TTCAATAAAA TTCTAAAATA 
AGTAATATCT AAATCATAAG CTCATCAAGT TATAAATAAA ACTGCAATTA AATTTTCATT 
AATAATTCAA CAATAACAAT AAATAATTAT TAAGTTACTA ATTAAGACTT TTCCAAGCAC 
AATTCGATTA TAACTATAAG TAAATCAGCT GAGATAACAT TGACCAACTT CGGTTTATCA 
TAGTGCACTT TAGGCAGAAG TTTTAGTATT CTATCTTAGC TAGATGACGT AAAACTAAAT 
CTAGACTCAG TAACAATTAG TGGAAATACC TGTGCATAAA ATGTTCCAGA TGACAGCATC 
GTTTCTTCTC TGTTTGTCGC AAGCCTATTT TCGGTTAAAA ATTTTTATTT CAATGATAAT 
GATATTTGTA ACACTGTTGT ATTCACAACA GCTGCCTCGA TTAAGCAAGC TAATCAAACA 
TTTAGCTTTA ATTAAATTAC AGTTTCAGGA AATACTTTTG TTACTAAAAC TTCATACATT 
TTATTTAATG CTCTTTATTC TATGATATCT AAGCCAGCTC ACCAGGTTAT ATTTAACAAT 
TTAATGGTTT CTAATAATAA GCTAAATATC ACCAACAGTC AAACTGACCA GCAAATTTCT 
AGTTTAATTT ATACAACCAA AATATCTGAT GTCACATTTA CTAACACTAA TATAGTGAAT 
CAAACTTACA TATTCCTATC TGTAGTTGAC ACATCTAACT AAGTGCAGAT CTCAAATTTC 
AATTGCACTA ACGAGCCAGC TTATAACAAA GATATTCCAT TCACTAAAAG AACTGCAGGA 
TGCTTATATT TTATAGAAGT ATCAAATTTA AATATTTCTA CACTTAATAT AATCAATAAA 
TAAACGATTG ATGAAAATTT AATCAGATTC GAGAGCTATT TTACTAAATA CATAACGATA 
AATGTACAAA ATGCTACATT TGCTAATAAT AATTAATCAC AGTTAGCAGG AAACGCCCCA 
ATGAACCCTC TTTATATAAT GATAATGTAT AATGCAACGA TTAATATCCT CAACAGCAAT 
TTCACTTCAA ATAATTATTA TATTAAGGAG AAATATCACG ACAAGTTCAT AGTTTCTACA 
AATGGGATTG GAACTCATTT CTTGTCAGGA AATTTAAATA TTAAGAACTG CAATTTTAAA 
AATTCAACAA GCAACGCTGG GTATAATTAT ATTTATGTCT AAGGTGATTC AATATATATG 
GAAAAATCAA CATTTATATA GTCCTCTTTT ACTGAAATAG TAACAAAATA AGCTTTCATT 
TTCAAAAATT ATGGTGGAAA TCTTTATCTT TAAGTGAAAA GCATCTTTTT TACAAAATGT 
TATTTCTCTT AATCAACTGC TGCATTTGGT TCATTCATTT ATGCATAACC TTTTTCCTCA 
ATTTTAGAAA TGTATATCTC AGATTCTACA TTTAATGAAG GTTATGCAAG TAATAACGGA 
GGAGCGATTT ACTTATATCC TTCAAATACA ATATAATTTA ATTGTGATAA CTGTAATTTC 
ACTAATATTT ACACTATAAG CGTAGCTTTA TCTGCAGCAA CTATTGGCTT AGAGAATTCA 
GCCTCATTAG ATACTTCGCG TCATTCTATT AATTTCAACG GTGGAGTAAT CAAAAATATA 
TATGGAAACG GAGACAATAA CTTCATTTAC CTCATAAAAG TTGACTTGCA AATCAATGAT 
TTCTAAACAA TAACTATTGA TAACTTTAAT TAATTGTTTA TATAACTTGT CAAGAAGGCT 
GGTACACTCT ATAGAATGGC GTCGCCAGCA TAACTAGTAA ATATTCAGAA TTCAAGCTTT 
TAAATGACAA ACTGCAATTT AACAAATTGG AGCCAAAATA GAAACACGTA GATGCCTCTT 
TTACTCTAAT CTCTTGATAG TAAGGTGACC ATAACAAATA GCTCTTTTAC TGATAGCAAA 
TTCAAATATT AAGTTATGAA AATAATAGGA GGATCAGTTT AATTTAGCTA AGTTACTTTC 
ACCAATTTTT CATAAATTGC TTTCAGCAGC TTTTCAGATT TATTTTCTAA TAGATTCTTA 
CAATAACTGA TTTAAAAATA ACCACTTTCA AGTGGAAATT CATTGATCTT AGTAAGTAAT 
AGTAAGCTCA TATTAAAAGA TACTTCTTTG CTCTCAAAAA TTAATTGTAC CTCAAATTGT 
TAAGGAAGTT CTCTTTAAAT ATTAAATACT ACTTTCACTA TCTAAGATAC ATCATTTTCA 
TAATCTAGTT CTAATTTTGG TGGAGCTATC TTTGTTTAAG CTTTTACTGG TTAGAATTAA 
ATAAAAAGTT GTAGTTTTAC TTAAAATACA GCAAAATATG ATGGTGGATC ATTGTATTTA 
AATGCTGGAG ACACTGATGT ATTCAATCTC ATTGTTGATT CAAACTCATT TACAGAAAAT 
ATATCCTAAA ATGGTAAAGG CGGAGCTATT TACATTTACA GCAGTTCACT AAATACTTTA 
AATTAAAATA TAACCATTTA CAATAACTAA ATTTAATAGA ATTAAGCTAT GGTGGGAGGA 
GCGTTGAGCA GTGAAAACAT AGCTCCCAAT AGCACTTAAA ATTAGATTAG TAACAACAAA 
GCCTTGATTT ATGGCAATAA TGAAATATCT TATCCAACTC AGCTAGGATT TTACAATTTA 
GATCACTTTT TAAAAGAAAA TAAAAATTCA ACTTACGTTG ATGGTTTGCT AACTATTAAG 
AATTTTAGAA GCGGAGATAA AATCCCTACT CTTGCCCTTT AGTTTAAAAA TGAAGAAAGC 
ATAATATATC CTTTATAAGA AAGCGAAATC AGCATGTATT CTATTTAAGT AGATGTTTAT 
CAAGCTTCTC AGAATGTTTA GTACGACTTA GTAGGAAAGC TTGTTTCAGT CTATGATAAA 
GATTCAAAAA GTTTTGTTTT TACTGATGTC CAAGCAGTAG GAACCCCTGA AACTTCTCAA 
TATTTTGAAT TTAAATCAAA CAATATTAAA ATTTTAGATC CAATCACATA TACTTATGTT 
TCAAACTATT CTTTAAAAAT TAAAATTGAA TTTAGATCAT GCTAAGCTGG TGAGGAAATT 
CTAAAATACA AGCAGCTAAC TGAATGTAGT CCTTGTGCAG AAAATACTTT TTCTTTAGAT 
CTGAACGGTT GTTAGCCGTG TCCTGATGGA GCAAAGTGTA GTGGAGGTTA AAATATTATA 
GTAGAATCTG GTTACTGGAG AAGTTCACTT TCTTCAAGTA AAGTAATAAA ATGCAATAAT 
GCCTTATCTA ATTGCCTAGA AGGCTCACAC GGAAATTAAA TTTGTTATGA AGGACATATT 
GGAGCATTAT GTGAAGAATG CGATACTCAA GGATAATTTT GGTTGGAAAG GTTTGCCAAG 
TCAGGAGATT ATAGTTGTAC AAACTGTAAT AAAATTACAG GAAATCTCTA TATAGTAATT 
TTGATGACAG TTTGGACTAT GATATCGATG ACTATCGCAA TCAGGGGTGA TATTGATACG 
TTAGAGAATG CAGCAGGAGC CTTAACCATT AAAAGATATA TTGAAAAAAA TGGGTCAAGT 
TCCATAGAAA AAAGACCTAC TCATGCATCT ATGATAAATA AGAAAAAATT TGGTAATAAT 
TAAGAAGATG AATAAAGAGC AGGTACTTAC GTCAAAATGC TAACAAATTA TTTGTAGATT 
GTAAATACTC TTGCTTCATT TAATCTCACT TTACCATCAG GTATTTACAG TGCTCCTGAA 
GCAGTTGGAA GACCTCTCTA ATAGTCAATG AATTCGTTGG ATTGTGCATT AGCAGAAATA 
AAAACAGATG TTCCTATTAT TTATTTAAGA GTCATGTTTT CACTAATCAT CCCATTAATT 
TATTTATTAT TATTCTTTTT TGGAATAGTT ATTGCTTAGT TATTTAATAG AGGAAAACCT 
TTTCCTTGGT ACATAGTTTC AACAGGAATA ATGTTCTTAA TTATTTATGT GCAGCCAGAT 
TTGGTTTCAT AAGTTATATC GTTATTATCA TGCAGAAAAA TCGGTGATGA AGAATATATT 
CTTGGAAATG TGTCTTACAT GTGCCATAAT GAATAACATA ATAAATACTC CTATTTCTTT 
TTAATTCCTG TATTATTCCT TTGGATATTT TTAATTCCAT TTATATTATG GTTACTTTTA 
TGGAGAAAGG TAAAAAGTTT AGATACTCTA TAAGTCAAAC TAAAATATGG ATTCCTCTAT 
AAAGAATATC AAAGATGGTA CTGGGAATTT GTAAAAATGG CAGAAAAAAT AGCTATTATA 
TTAGTTTTGA ATTTCTTTTA TTCAAATGTA CCAGTAAAAG GGTTGATCTC TTTTTAGATC 
ATCGTGATAT ATGGTCTCTT AGCTCAAAGA TTTAATCCAT ATAAAGAAGA ATTTATTAAC 
AAACTGGATG TGTACTCAAC TAACTGCTCT GCAGCTAGTA TATTGATTGG TGTTTTCATA 
TATGACAATT CATATAACTA TCTAGTAATT ATAGGTTTTA TAATGCTAAT TATTTTAAAT 
GCTTGGTACA TAATCAGTCT AGTGATTAAA ATTTTAAGTG GATATATATA TAAGATCAAA 
GGCATCAAAA AAATTATAAT AGAGAAATTA GCAGAAAAAA TTGACTACTT TAAAAAATAC 
CTTCCTAAAG AAGAATAAAA AAGAAAAATG AATCCTGAAG TCCGCTAAAA AATGAGAAAT 
ATTTTCAATA AATTCATAAA ACTTCCTCCA GCTGAAAAGA AAGCTGTAAT CCTTAAGATT 
TATGAAGAGC AATTAACCGA TTTGAATAAT TAAGAATATG ATAAAGCCAA GAAGAAAGTA 
AACTAATTTT TTGAAAGAGA GTCTCAGAAT TTGAATTAAA AAGCTTTTTC TGAAACTACA 
GTGTAGAGGA AAAGTGATAA AATAGAATTA CAATAAAAAG CTTTAGATGT TTAAAATATA 
AAATTCGAAT AGGTTACTGA AATGAACGAT GAAAAAAATG TATAATTGAT CGTATTTGAA 
CCAGATTGTA GCTAATTGAG TGCACCAAAA AAAACAGAAA CTTCGTCAAT TTTTTAGAAA 
AGAGTTTCTA TTAAAAAAAT AGACTCTGAC TAAACTCCTA AAATATAATA AGAATCCCCA 
TTTAATGGAT TTGATAGTTT ACAAAATCCT GAAATTAATA AATTAATAAA AATAGAGTGT 
TAAAAAATGT AGAGCTCAGA CTTAAATTTA ATATAAAAAA TAAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001010189.2
CDS	1..7728
Translation	MRMKNLKHLVLLFITIINLKIFSECPSIARYLNILPQYYARSFLKLPKTNLLVVNTVSDVIGSSVVYYIDISMNPGIVITTIRPDFLITMEYMLSQNSILVANNNKIIIANPYTLASNAVLNIVDFFVNTNYIVVTLAYNIVILDSSSLKTIKILDNTSFLSKKGVLISTQFYTLSFQNFIICSDEGLYAWKVNFESEFSYNGYIPDTLNSSIKKFEKHPKYDILIVGGLKNVKFIIDQKYTTLSITVNFSSVESIIYSINSSDNIIIGDSQSIFIYFSVNPVDNTIESFKLDYVENHYDAYKLIELPIIVYASNNLVTFYNYQSKSLSYNLNFSSDYINKSFIFNSTKEDLFLTINQNNLVVYQRSNLGVQYMQKALSAVL*QNSFYRVKNCGNCFFIKTSDSSNKNYIVYTKFDDIKSPSFYDIGTVSLTWSINNLDPFILNDLYVVFSQPNITLPGENYYFYLLQFSAVDLSIADVKLTTNNNRTSLAVYDSLNKILYGIDSGGTVYKYDINKNLVQSFINGCLNAYIGEIFIDPYFLIFGCNNDIIAYNLVSKTSIVTNLYVSPFVLVFSSSSIIALGDIDRGSAKIFKLNTISQSFDFFIEIFSSKGVDILLSVDLLSDSNLLLFKNGIIFFQFSSCLDINNCLGCNNYYFKIQDGLDSKSYGQGKGVEFITSSFLTAVLKAQIYKNLVRLTSLDVNMYIDKANPFILNSGLLIFDFSNIISLSMQCTSC*SEYFSLYSSDLLLNFVQFNLNVSVNFTNLSYSQSCGLKINIQNSKISNIIISSSSYKNCNIFINNSTITINNYVTNDFSKHNSIITISKSAEITLTNFGLSCTLGRSFSILSLDDVKLNLDSVTISGNTCANVPDDSIVSSLFVASLFSVKNFYFNDNDICNTVVFTTAASIKQANQTFSFNITVSGNTFVTKTSYILFNALYSMISKPAHQVIFNNLMVSNNKLNITNSQTDQQISSLIYTTKISDVTFTNTNIVNQTYIFLSVVDTSNVQISNFNCTNEPAYNKDIPFTKRTAGCLYFIEVSNLNISTLNIINKTIDENLIRFESYFTKYITINVQNATFANNNSQLAGNAPMNPLYIMIMYNATINILNSNFTSNNYYIKEKYHDKFIVSTNGIGTHFLSGNLNIKNCNFKNSTSNAGYNYIYVGDSIYMEKSTFISSFTEIVTKAFIFKNYGGNLYLVKSIFFTKCYFSSTAAFGSFIYAPFSSILEMYISDSTFNEGYASNNGGAIYLYPSNTIFNCDNCNFTNIYTISVALSAATIGLENSASLDTSRHSINFNGGVIKNIYGNGDNNFIYLIKVDLQINDFTITIDNFNLFILVKKAGTLYRMASPALVNIQNSSFMTNCNLTNWSQNRNTMPLLLSLDSKVTITNSSFTDSKFKYVMKIIGGSVFSVTFTNFSIAFSSFSDLFSNRFLQLIKPLSSGNSLILVSNSKLILKDTSLLSKINCTSNCGSSLILNTTFTIDTSFSSSSNFGGAIFVAFTGNIKSCSFTNTAKYDGGSLYLNAGDTDVFNLIVDSNSFTENISNGKGGAIYIYSSSLNTLNNITIYNNI*NAMVGGALSSENIAPNSTNISNNKALIYGNNEISYPTQLGFYNLDHFLKENKNSTYVDGLLTIKNFRSGDKIPTLALFKNEESIIYPLESEISMYSIVDVYQASQNVYDLVGKLVSVYDKDSKSFVFTDVQAVGTPETSQYFEFKSNNIKILDPITYTYVSNYSLKIKIEFRSCAGEEILKYKQLTECSPCAENTFSLDLNGCPCPDGAKCSGGNIIVESGYWRSSLSSSKVIKCNNALSNCLEGSHGNICYEGHIGALCEECDTQGFWLERFAKSGDYSCTNCNKITGNLYIVILMTVWTMISMTIAIRGDIDTLENAAGALTIKRYIEKNGSSSIEKRPTHASMINKKKFGNNEDERAGTYVKMLTNYLIVNTLASFNLTLPSGIYSAPEAVGRPL*SMNSLDCALAEIKTDVPIIYLRVMFSLIIPLIYLLLFFFGIVIALFNRGKPFPWYIVSTGIMFLIIYVQPDLVSVISLLSCRKIGDEEYILGNVSYMCHNEHNKYSYFFLIPVLFLWIFLIPFILWLLLWRKVKSLDTLVKLKYGFLYKEYQRWYWEFVKMAEKIAIILVLNFFYSNVPVKGLISFIIVIYGLLAQRFNPYKEEFINKLDVYSTNCSAASILIGVFIYDNSYNYLVIIGFIMLIILNAWYIISLVIKILSGYIYKIKGIKKIIIEKLAEKIDYFKKYLPKEEKRKMNPEVRKMRNIFNKFIKLPPAEKKAVILKIYEEQLTDLNNEYDKAKKKVNFFERESQNLNKAFSETTVRKSDKIELQKALDVNIKFEVTEMNDEKNVLIVFEPDCSLSAPKKTETSSIFKRVSIKKIDSDTPKIESPFNGFDSLQNPEINKLIKIECKMSSDLNLIKIN

Target ORF information:

RefSeq Version	XM_001010189.2
Organism	Tetrahymena thermophila SB210
Definition	Tetrahymena thermophila SB210 transmembrane protein, putative partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001010189.2

ORF Insert Sequence:

ATGAGAATGA AGAATTTAAA ATAGCATTTA GTACTGCTAT TTATTACAAT TATAAATTTA 
AAATAAATAT TTAGTTAAGA ATGTCCAAGT ATTGCAAGAT ATTTAAATAT CCTTCCTCAA 
TATTATGCAA GAAGCTTTTT GAAATTACCA AAAACCAACC TTTTAGTAGT TAATACAGTA 
TCTGATGTGA TTTAGGGATC TAGCGTAGTT TACTATATTG ACATTTCAAT GAACCCAGGA 
ATAGTTATTA CAACAATAAG GCCTGATTTT TTAATTACTT AAATGGAATA CATGCTCTCC 
CAAAACAGTA TATTGGTAGC TAACAACAAT AAAATTATCA TAGCTAATCC TTACACGCTT 
TAAGCTTAAA GTAATGCTGT ATTGAATTAG ATAGTAGACT TTTTTTAAGT ATAGAACACA 
AATTATATTG TTGTCACTCT AGCTTACAAT TAAATTGTTA TTTTAGACTC TTCAAGCTTA 
AAAACTATAA AGATATTGGA TAATACTAGC TTTCTAAGTA AAAAGGGTGT ATTATAAATA 
TCAACTCAAT TTTATACTCT TTCTTTTTAA CAGAATTTTA TAATTTGCTC TGATGAGTAA 
GGTTTGTATG CCTGGAAGGT CAATTTTGAG TAATCAGAGT TTAGTTACAA TGGATATATC 
CCTGATACTT TGAATTCCTC GATCAAAAAA TTTGAAAAAC ACCCAAAATA TGATATCTTA 
ATTGTAGGTG GATTAAAGAA TGTTAAATTC ATTTAAATAT AAGACCAAAA ATACACTACG 
TTATCAATAT AAACAGTTAA TTTTAGCAGT GTTGAAAGCA TTATATACTA GAGCATTAAT 
TCATCAGATA ATATAATTAT AGGAGATTCT CAGAGTATTT TCATTTACTA GTTTAGTGTA 
AACCCTGTTG ATAATACAAT AGAATCTTTT AAGTTGGATT ATTAAGTTTA ATAAGAAAAT 
CATTATGACG CCTACAAGCT TATTGAACTC CCAATTATTG TTTATGCAAG CAATAATTTA 
GTTACATTTT ATAATTACCA AAGCAAAAGT TTGAGCTATA ATTTAAACTT TTCTAGTGAT 
TACATAAATA AAAGCTTTAT TTTTTAGAAT TCTACTAAAT AGGAAGATTT ATTCTTAACT 
ATTAATCAGA ATAATTTAGT TGTGTATCAA AGAAGCAATT TAGGAGTTCA GTATTAGATG 
CAAAAATAGG CTTTGTAATC AGCTGTTCTT TAGTAGCAAA ATAGTTTTTA TAGAGTTAAA 
AACTGTGGAA ATTGCTTTTT CATCAAAACA AGTGATAGTT CTAATAAAAA TTATATTGTT 
TATACAAAAT TTGATGATAT TAAAAGTCCT TCATTCTAAT ACGATATTGG AACAGTTTCA 
TTAACTTGGA GTTAAATAAA CTAAAATTTA GATCCTTTTA TTTTAAATGA TTAACTATAT 
GTTGTTTTTT CTCAACCAAA CTAGATTACA CTTCCATAAG GAGAGAATTA TTACTTTTAT 
TTATTGCAGT TCTCGGCGGT AGATTTATCT TAAATTGCAG ATGTAAAGCT CACTACAAAT 
TAAAATTAAA ATAGAACCAG CTTAGCTGTT TATGATTCTT TGAATAAAAT TTTATATGGC 
ATAGACTCAG GAGGTACAGT CTATAAATAT GATATCTAAA ATAAAAACTT GGTTCAAAGC 
TTTTAAATTA ATGGCTGCTT AAATGCTTAT ATTGGAGAGA TTTTCATATA AGATCCTTAT 
TAATTCTTAA TTTTTGGATG TTAAAACAAT GATATTATAG CATATAACTT AGTAAGTAAA 
ACTTAAAGCA TTGTAACAAA TTTATACGTA TCTCCTTTTG TCCTGTAAGT ATTTAGTAGT 
TCCTCAATTA TTGCTCTTGG AGATATTGAT AGAGGATCTG CAAAAATTTT CAAGCTTAAT 
ACTATTTCCC AAAGTTTTGA TTTTTTTATA GAAATATTCT CTAGTAAGGG AGTGGACATC 
CTTCTATCTG TAGATTTATT AAGTGACTCT AATTTATTAC TTTAATTTAA AAATGGCATC 
ATATTCTTCC AATTTAGCTC TTGCTTGTAG GATATTAACA ATTGTTTGGG ATGCAACTAA 
AATTATTATT TCAAAATTCA AGATGGTCTT GATTCTAAAT AAAGCTATGG GCAAGGTAAA 
TAGGGTGTAG AATTTATTAC ATCTTAAAGC TTCCTCACAG CTGTTTTGAA GGCTCAAATA 
TACAAAAATC TAGTAAGATA ACTTACTTCT TTGGATGTCA ATATGTATAT CGATAAGGCT 
AATCCATTTA TTTTAAACTC CGGTTTACTC ATTTTTGACT TTAGCAATAT TATTTCTTTA 
TCTATGCAAT GCACAAGTTG TTAATAAAGT GAGTATTTTA GTTTATATTC ATCAGATTAG 
TTGCTATTAT AAAACTTTGT TCAATTTAAT TTGAATTAAG TTTCAGTGAA CTTTACAAAT 
TTAAGTTATA GTCAGAGCTG TGGCCTAAAA ATTTAAAATA TTCAATAAAA TTCTAAAATA 
AGTAATATCT AAATCATAAG CTCATCAAGT TATAAATAAA ACTGCAATTA AATTTTCATT 
AATAATTCAA CAATAACAAT AAATAATTAT TAAGTTACTA ATTAAGACTT TTCCAAGCAC 
AATTCGATTA TAACTATAAG TAAATCAGCT GAGATAACAT TGACCAACTT CGGTTTATCA 
TAGTGCACTT TAGGCAGAAG TTTTAGTATT CTATCTTAGC TAGATGACGT AAAACTAAAT 
CTAGACTCAG TAACAATTAG TGGAAATACC TGTGCATAAA ATGTTCCAGA TGACAGCATC 
GTTTCTTCTC TGTTTGTCGC AAGCCTATTT TCGGTTAAAA ATTTTTATTT CAATGATAAT 
GATATTTGTA ACACTGTTGT ATTCACAACA GCTGCCTCGA TTAAGCAAGC TAATCAAACA 
TTTAGCTTTA ATTAAATTAC AGTTTCAGGA AATACTTTTG TTACTAAAAC TTCATACATT 
TTATTTAATG CTCTTTATTC TATGATATCT AAGCCAGCTC ACCAGGTTAT ATTTAACAAT 
TTAATGGTTT CTAATAATAA GCTAAATATC ACCAACAGTC AAACTGACCA GCAAATTTCT 
AGTTTAATTT ATACAACCAA AATATCTGAT GTCACATTTA CTAACACTAA TATAGTGAAT 
CAAACTTACA TATTCCTATC TGTAGTTGAC ACATCTAACT AAGTGCAGAT CTCAAATTTC 
AATTGCACTA ACGAGCCAGC TTATAACAAA GATATTCCAT TCACTAAAAG AACTGCAGGA 
TGCTTATATT TTATAGAAGT ATCAAATTTA AATATTTCTA CACTTAATAT AATCAATAAA 
TAAACGATTG ATGAAAATTT AATCAGATTC GAGAGCTATT TTACTAAATA CATAACGATA 
AATGTACAAA ATGCTACATT TGCTAATAAT AATTAATCAC AGTTAGCAGG AAACGCCCCA 
ATGAACCCTC TTTATATAAT GATAATGTAT AATGCAACGA TTAATATCCT CAACAGCAAT 
TTCACTTCAA ATAATTATTA TATTAAGGAG AAATATCACG ACAAGTTCAT AGTTTCTACA 
AATGGGATTG GAACTCATTT CTTGTCAGGA AATTTAAATA TTAAGAACTG CAATTTTAAA 
AATTCAACAA GCAACGCTGG GTATAATTAT ATTTATGTCT AAGGTGATTC AATATATATG 
GAAAAATCAA CATTTATATA GTCCTCTTTT ACTGAAATAG TAACAAAATA AGCTTTCATT 
TTCAAAAATT ATGGTGGAAA TCTTTATCTT TAAGTGAAAA GCATCTTTTT TACAAAATGT 
TATTTCTCTT AATCAACTGC TGCATTTGGT TCATTCATTT ATGCATAACC TTTTTCCTCA 
ATTTTAGAAA TGTATATCTC AGATTCTACA TTTAATGAAG GTTATGCAAG TAATAACGGA 
GGAGCGATTT ACTTATATCC TTCAAATACA ATATAATTTA ATTGTGATAA CTGTAATTTC 
ACTAATATTT ACACTATAAG CGTAGCTTTA TCTGCAGCAA CTATTGGCTT AGAGAATTCA 
GCCTCATTAG ATACTTCGCG TCATTCTATT AATTTCAACG GTGGAGTAAT CAAAAATATA 
TATGGAAACG GAGACAATAA CTTCATTTAC CTCATAAAAG TTGACTTGCA AATCAATGAT 
TTCTAAACAA TAACTATTGA TAACTTTAAT TAATTGTTTA TATAACTTGT CAAGAAGGCT 
GGTACACTCT ATAGAATGGC GTCGCCAGCA TAACTAGTAA ATATTCAGAA TTCAAGCTTT 
TAAATGACAA ACTGCAATTT AACAAATTGG AGCCAAAATA GAAACACGTA GATGCCTCTT 
TTACTCTAAT CTCTTGATAG TAAGGTGACC ATAACAAATA GCTCTTTTAC TGATAGCAAA 
TTCAAATATT AAGTTATGAA AATAATAGGA GGATCAGTTT AATTTAGCTA AGTTACTTTC 
ACCAATTTTT CATAAATTGC TTTCAGCAGC TTTTCAGATT TATTTTCTAA TAGATTCTTA 
CAATAACTGA TTTAAAAATA ACCACTTTCA AGTGGAAATT CATTGATCTT AGTAAGTAAT 
AGTAAGCTCA TATTAAAAGA TACTTCTTTG CTCTCAAAAA TTAATTGTAC CTCAAATTGT 
TAAGGAAGTT CTCTTTAAAT ATTAAATACT ACTTTCACTA TCTAAGATAC ATCATTTTCA 
TAATCTAGTT CTAATTTTGG TGGAGCTATC TTTGTTTAAG CTTTTACTGG TTAGAATTAA 
ATAAAAAGTT GTAGTTTTAC TTAAAATACA GCAAAATATG ATGGTGGATC ATTGTATTTA 
AATGCTGGAG ACACTGATGT ATTCAATCTC ATTGTTGATT CAAACTCATT TACAGAAAAT 
ATATCCTAAA ATGGTAAAGG CGGAGCTATT TACATTTACA GCAGTTCACT AAATACTTTA 
AATTAAAATA TAACCATTTA CAATAACTAA ATTTAATAGA ATTAAGCTAT GGTGGGAGGA 
GCGTTGAGCA GTGAAAACAT AGCTCCCAAT AGCACTTAAA ATTAGATTAG TAACAACAAA 
GCCTTGATTT ATGGCAATAA TGAAATATCT TATCCAACTC AGCTAGGATT TTACAATTTA 
GATCACTTTT TAAAAGAAAA TAAAAATTCA ACTTACGTTG ATGGTTTGCT AACTATTAAG 
AATTTTAGAA GCGGAGATAA AATCCCTACT CTTGCCCTTT AGTTTAAAAA TGAAGAAAGC 
ATAATATATC CTTTATAAGA AAGCGAAATC AGCATGTATT CTATTTAAGT AGATGTTTAT 
CAAGCTTCTC AGAATGTTTA GTACGACTTA GTAGGAAAGC TTGTTTCAGT CTATGATAAA 
GATTCAAAAA GTTTTGTTTT TACTGATGTC CAAGCAGTAG GAACCCCTGA AACTTCTCAA 
TATTTTGAAT TTAAATCAAA CAATATTAAA ATTTTAGATC CAATCACATA TACTTATGTT 
TCAAACTATT CTTTAAAAAT TAAAATTGAA TTTAGATCAT GCTAAGCTGG TGAGGAAATT 
CTAAAATACA AGCAGCTAAC TGAATGTAGT CCTTGTGCAG AAAATACTTT TTCTTTAGAT 
CTGAACGGTT GTTAGCCGTG TCCTGATGGA GCAAAGTGTA GTGGAGGTTA AAATATTATA 
GTAGAATCTG GTTACTGGAG AAGTTCACTT TCTTCAAGTA AAGTAATAAA ATGCAATAAT 
GCCTTATCTA ATTGCCTAGA AGGCTCACAC GGAAATTAAA TTTGTTATGA AGGACATATT 
GGAGCATTAT GTGAAGAATG CGATACTCAA GGATAATTTT GGTTGGAAAG GTTTGCCAAG 
TCAGGAGATT ATAGTTGTAC AAACTGTAAT AAAATTACAG GAAATCTCTA TATAGTAATT 
TTGATGACAG TTTGGACTAT GATATCGATG ACTATCGCAA TCAGGGGTGA TATTGATACG 
TTAGAGAATG CAGCAGGAGC CTTAACCATT AAAAGATATA TTGAAAAAAA TGGGTCAAGT 
TCCATAGAAA AAAGACCTAC TCATGCATCT ATGATAAATA AGAAAAAATT TGGTAATAAT 
TAAGAAGATG AATAAAGAGC AGGTACTTAC GTCAAAATGC TAACAAATTA TTTGTAGATT 
GTAAATACTC TTGCTTCATT TAATCTCACT TTACCATCAG GTATTTACAG TGCTCCTGAA 
GCAGTTGGAA GACCTCTCTA ATAGTCAATG AATTCGTTGG ATTGTGCATT AGCAGAAATA 
AAAACAGATG TTCCTATTAT TTATTTAAGA GTCATGTTTT CACTAATCAT CCCATTAATT 
TATTTATTAT TATTCTTTTT TGGAATAGTT ATTGCTTAGT TATTTAATAG AGGAAAACCT 
TTTCCTTGGT ACATAGTTTC AACAGGAATA ATGTTCTTAA TTATTTATGT GCAGCCAGAT 
TTGGTTTCAT AAGTTATATC GTTATTATCA TGCAGAAAAA TCGGTGATGA AGAATATATT 
CTTGGAAATG TGTCTTACAT GTGCCATAAT GAATAACATA ATAAATACTC CTATTTCTTT 
TTAATTCCTG TATTATTCCT TTGGATATTT TTAATTCCAT TTATATTATG GTTACTTTTA 
TGGAGAAAGG TAAAAAGTTT AGATACTCTA TAAGTCAAAC TAAAATATGG ATTCCTCTAT 
AAAGAATATC AAAGATGGTA CTGGGAATTT GTAAAAATGG CAGAAAAAAT AGCTATTATA 
TTAGTTTTGA ATTTCTTTTA TTCAAATGTA CCAGTAAAAG GGTTGATCTC TTTTTAGATC 
ATCGTGATAT ATGGTCTCTT AGCTCAAAGA TTTAATCCAT ATAAAGAAGA ATTTATTAAC 
AAACTGGATG TGTACTCAAC TAACTGCTCT GCAGCTAGTA TATTGATTGG TGTTTTCATA 
TATGACAATT CATATAACTA TCTAGTAATT ATAGGTTTTA TAATGCTAAT TATTTTAAAT 
GCTTGGTACA TAATCAGTCT AGTGATTAAA ATTTTAAGTG GATATATATA TAAGATCAAA 
GGCATCAAAA AAATTATAAT AGAGAAATTA GCAGAAAAAA TTGACTACTT TAAAAAATAC 
CTTCCTAAAG AAGAATAAAA AAGAAAAATG AATCCTGAAG TCCGCTAAAA AATGAGAAAT 
ATTTTCAATA AATTCATAAA ACTTCCTCCA GCTGAAAAGA AAGCTGTAAT CCTTAAGATT 
TATGAAGAGC AATTAACCGA TTTGAATAAT TAAGAATATG ATAAAGCCAA GAAGAAAGTA 
AACTAATTTT TTGAAAGAGA GTCTCAGAAT TTGAATTAAA AAGCTTTTTC TGAAACTACA 
GTGTAGAGGA AAAGTGATAA AATAGAATTA CAATAAAAAG CTTTAGATGT TTAAAATATA 
AAATTCGAAT AGGTTACTGA AATGAACGAT GAAAAAAATG TATAATTGAT CGTATTTGAA 
CCAGATTGTA GCTAATTGAG TGCACCAAAA AAAACAGAAA CTTCGTCAAT TTTTTAGAAA 
AGAGTTTCTA TTAAAAAAAT AGACTCTGAC TAAACTCCTA AAATATAATA AGAATCCCCA 
TTTAATGGAT TTGATAGTTT ACAAAATCCT GAAATTAATA AATTAATAAA AATAGAGTGT 
TAAAAAATGT AGAGCTCAGA CTTAAATTTA ATATAAAAAA TAAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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TTHERM_00561350 cDNA ORF clone, Tetrahymena thermophila SB210

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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