LOC101071039 cDNA ORF clone, Takifugu rubripes(Fugu rubripes)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101071039
Entrez Gene ID	101071039
Full Name	mitogen-activated protein kinase kinase kinase 5-like
Gene Type	protein-coding
Organism	Takifugu rubripes(Fugu rubripes)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011605057.2	XP_011603359.2	mitogen-activated protein kinase kinase kinase 5-like isoform X1
XM_011605058.1	XP_011603360.1	mitogen-activated protein kinase kinase kinase 5-like isoform X2
XM_011605058.2	XP_011603360.2	mitogen-activated protein kinase kinase kinase 5-like isoform X2
XM_011605057.1	XP_011603359.1	mitogen-activated protein kinase kinase kinase 5-like isoform X1

Pathways from BioSystems
Gene Ontology

The following LOC101071039 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101071039 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OTo25875	XM_011605057.1 Latest version!	Takifugu rubripes mitogen-activated protein kinase kinase kinase 5-like (LOC101071039), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OTo25876	XM_011605058.1 Latest version!	Takifugu rubripes mitogen-activated protein kinase kinase kinase 5-like (LOC101071039), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OTo69903	XM_011605057.2 Latest version!	Takifugu rubripes mitogen-activated protein kinase kinase kinase 5-like (LOC101071039), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OTo69904	XM_011605058.2 Latest version!	Takifugu rubripes mitogen-activated protein kinase kinase kinase 5-like (LOC101071039), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101071039 ( XM_011605057.1 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_011603359.1 Takifugu rubripes mitogen-activated protein kinase kinase kinase 5-like (LOC101071039), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OTo25875
Clone ID Related Accession (Same CDS sequence)	XM_011605057.1
Accession Version	XM_011605057.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3975bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1426348800000
Organism	Takifugu rubripes(Fugu rubripes)
Product	mitogen-activated protein kinase kinase kinase 5-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070976.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACACGA CGGAGCGCAG GCGAATGAGC CTGCATGATC TGAAGAGGAG GGATCCGCTT 
CGGGTGTCAG CTGGAAGTTT GTGGCAGGAC TCGCTGGTCA TGGATCCGGG TGCCAACGGC 
GCGGTGAAAC ACGTCGTGTC TCCGAGGAGT CGGACCAGAG CCATATCCGT GGCGTACATC 
GTCAACGAGG ACGCGTCGGT GCCGCAGACT GAGGAGAACC TCTCACTGAC GTGTCTGAAG 
GACGCATGTG CGGACGCGCA CGCCTCTTTA CATACTATTG CATTTGAGAG AATAACCTTG 
GGGACGACAG ACGTCCTGGA TAGTTTCTAC AACGCAGACG TAGCGGTGGC GGAGATGAGC 
GACTCTTTCT GCCAGCCTTC CCTTTTCTAT CACCTCGGAG TGAGAGAGAG TTTCAGCATG 
ACCAACAACA TCATTTTATA CTGTTACAAG CAGGATAGCG ACCTGCAGGC TATCAAGGAG 
CAGTGTGGGA GTTTCACATT CATCCCGTAC GTGGTGTCGC CTCAGGGCAA AGTGTTTGCC 
TGCGATGCTC GCATGATGAC GTGTATGAAA GAGTTGATGC AGCCCAGCTT CCAGCTGGAG 
CCCCTGCTGA CGCCGCTGGT GGAGCGACTG GTGCATCTTC TCAGCAGTGT GCACATTCAA 
TCCAGCGAGT ACTTCAGGGA GTCCATCAGG CAGGAGATCC GAATGGCCCG GGAGCGTTTC 
AGCGGGCAGG ACCTGAGCAA GGAACTGAGA CGCATCCAGA AACGCCTGGA CAGCGTGGAG 
CTGCTCACCC CAGATATCGT CATCAACCTG CTGCTGTCTT ACAGGGACAT TCAGGATTAT 
GATTCCATAA TCAATCTGGT GGAGACTCTG AACAACCTGC CCATGTGTCT GATAGCGCAG 
CATCAGAATA TAAAGTTTCA CTACATATTT GCTCTAAACA GGAGGAATCA CCCCGGAGAC 
CGTGCCAAGG CTTTGCAGTT GATCCTGCCG CTTGTGGAAT CCAGTGACAA GGTGGCGTCG 
GACATCTACT GCCTGTGTGG ACGGATCTAC AAGGACATGT TCATGAGCTC TGGCTTCAGT 
GATCAGAGCA GCAGAGATCA GGCTTGCTGC TGGTATGGCA AAGCTTTTGA AACGGAACCG 
ACTCTTCACT CGGGCATCAA CGTCGTCGTG CTTCTGATGG CAGCCGGCCA TGAATTTGAG 
ACTTCCATCG AGATCCGTAA AATCGGGGTG ACTCTGAACA CTTTGCTGGG GCGGAAGGGC 
AGTTTGGAGA AGATGAAGGA CTACTGGGAT GTCGGCTTCT ACCTCGGAGC GAACATCCTC 
ACTAACGAAC ACAGGAAAGT CATTGAAGCC TGTGAAAAAC TTTACCGGCT GAAGGCTCCC 
GTGTGGTACG TGGCTTCAAT CATGGAGACC TTCATCCTGT TTCGACAGTT TGCCAAACTA 
CCTGAGGTGA AATCGCCCAA ACAGGAGATT ATGGACTTCT GGATGGAGCT GCTTCTGCAG 
ACGTACAAAC CCACAGTTTC CACCGGCCGT TGCCCAGTGC TCGTTCTGGA GCCCAGTAAA 
GTCCTCCAGC CGGCCATTTT GTGCGTGAGC GAGGAGGATG AAAATCGCAC GGTTCAGCTG 
AAACACATCA CAACCCAGAA GAAAGGCTTA CACCAGTGGA CGTTCCCAGC CTCTGCGATT 
CGGGGAGTGA GTGCTTCAAA GATTGATGAG CGGAGTTGCT TCCTGTATGT CCACTACAAC 
TCAGATGACT TCCAGCTCTG TTTCCCCTCT CAGCTTCACT GTAATGGTTT CTGTGAGCTG 
GTGAACTCTC TCCTTCAGCA GGCTGAAGAT TCGTCCACAC AACTGGATCA TCTTCCCGAG 
GGGGTGATCG AGTATGTTTA CGAGACTGAT GAGAATGGTG ACAACGTGGT TCTGGGTAAA 
GGCACGTATG GCGTGGTGTA CGCGGGGAGG GACCTGAGCA ACCAAGTACG CATCGCCATC 
AAGGAAATCC CTGAAAAGGA CAGCATGTAC TCGCAGCCCC TTCATGAAGA AATAGCTCTG 
CATAAGAGGC TAAAACACAG GAACATTGTG CAGTACCTGG GCTCCGTCAG TCAGGATGGA 
TTCATCAAGA TCTTCATGGA GGAAGTACCT GGAGGAAGTT TGTCCTCTCT GCTGCGCTCC 
AAGTGGGGCC CTCTGAAAGA CAATGAAGCA ACCATCATCT TCTACACCAA ACAGATCCTC 
GAGGGGCTCA GATATCTTCA CGCTAATCAA ATTGTCCACC GGGACATCAA GGGCGACAAC 
GTGTTGATCA ACACCTACAG CGGAGTGTTG AAGATCTCCG ACTTTGGGAC CTCCAAACGA 
TTAGCTGGGA TCAACCCCTG CACCGAGACC TTTACTGGAA CTCTGCAATA CATGGCCCCA 
GAGATTATAG ACCAGGGACC TCGGGGTTAT GGGAAACCAG CTGATATCTG GTCTCTGGGG 
TGCACTATTA TTGAAATGGC AACAGGCAAA ACCCCATTCC ATGAACTGGG AAGTCCTCAG 
GCAGCCATGT TTAAGGTGGG CATGTTTAAG ATCCACCCCA AGGTTCCAGA ATGTATGTCA 
GATGAAGCAA AGGCGTTCAT CATGAACTGT TTCACGCCGA ACCCCGATGA CCGAGCCACA 
GCTTCAAAGC TGCTAATGGA TGCCTTCCTC AGGTTACAGC CCAGGAAGAA GCCCAAAGCT 
GTGCAGGAGC CAGACCCCAA AGACTTCCTT AGTATTGCTG AATATCAACG CAGCCTCTCT 
GTCCCGATCT CCATCTTTGT GGAGGACATT GATTCGCCCT CCAATTCGAT CGACCTGTCC 
GCTTCCCTGG ACCTGAGGTG GCACTTGTCA GCCCTCAAGA CGAATGACAT ATCAGAGAGT 
CCGCCTGGCA GCAGCTTCCT GCCACTACCA GAAGACTCTC CATCAGAAAT GCAGTGCAGC 
CCAGTATCAA CTGAGGAGAG TGTTGGTCTG TTTATGCTCA GAAAGGACAG TGAAAGGAGG 
GCCACGCTTC ACCGTGTCCT CACCGACTAC AACAATCTTG TTATTTCAAA TATACAGGAA 
TCTGTGCCTC AGCATAGTGA AGGATCATCT CTAAGTGCAG ACCACATCAG TGAGCTCATT 
TGCTGCTTGC GGGAAAACAT CCGCTCCCCG GACAGGATGC AGCTGACCAG TCGCCTGCTG 
GAACTTCGGA CTAAGCTGCT CAATGCCGCA ATACCGGCAA ATAGCCTCCA GGGGGTGCTA 
TTCAGCTTCC AAGATGCTGT GAAAAAGGTT TTGAAGCAAC AGCAGGTGAA ACCTCACTGG 
ATGTTCGCCC TGGACAATCT ACTGAGACAG GCAGTCCAGG ACGCCATCAC TGTGCTGCTT 
CCACAGCTGA AACTCCAGCT GCAGTCCTCA TTTGAGGTGG AGGACAGTAA TCCAGAAGAG 
CAGCGTACAA GCCCGGACAC TCCAGTTGTT CTTGTTCCTG ACCAGCTGGT GGCGCCAGCA 
GACACACAGA CGACTCCCAC ACAAGAGAAT GTCCAAACAG GAAGGCCCAA CTACCCCATA 
GACCTCGACC TCGACAGCAA TTGCCCCCTC AGTGAGACCT TAAGAAACCT ACGACTGGAG 
ACTTTGGGAC TGCTGAGTCA GTTGAATGAG CAGGAGAGAG AGTACCAGGA GCTACTGAGG 
AGCTCCGTCT GCAGGAAACA GGAACAGATT GATGCACTGA GGACTGCAGC CGCTGCCACC 
GAAGATGACT CATTGGTATC GCACACATAC TCAGATCCTG AAGCCAAGGC TTTGGTGTGC 
TGGCTCAAGT CCATCCCAGT TGATCAAGAC TCAATTAATA AGTTGCTCTC TCACAGGTTC 
ACTTTGGACT GTCTCCTCTG TCTGGCCTCC AGGGATGACT TGATGTATTG TGGAATAAGA 
GGTGGCATGT TGTGTCGAAT TTGGGCAGCC ATCACAGCGC ACAGGAATAA CCAGCTCAAC 
AAAGGGAAGG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011603359.1
CDS	177..4151
Translation	MYTTERRRMSLHDLKRRDPLRVSAGSLWQDSLVMDPGANGAVKHVVSPRSRTRAISVAYIVNEDASVPQTEENLSLTCLKDACADAHASLHTIAFERITLGTTDVLDSFYNADVAVAEMSDSFCQPSLFYHLGVRESFSMTNNIILYCYKQDSDLQAIKEQCGSFTFIPYVVSPQGKVFACDARMMTCMKELMQPSFQLEPLLTPLVERLVHLLSSVHIQSSEYFRESIRQEIRMARERFSGQDLSKELRRIQKRLDSVELLTPDIVINLLLSYRDIQDYDSIINLVETLNNLPMCLIAQHQNIKFHYIFALNRRNHPGDRAKALQLILPLVESSDKVASDIYCLCGRIYKDMFMSSGFSDQSSRDQACCWYGKAFETEPTLHSGINVVVLLMAAGHEFETSIEIRKIGVTLNTLLGRKGSLEKMKDYWDVGFYLGANILTNEHRKVIEACEKLYRLKAPVWYVASIMETFILFRQFAKLPEVKSPKQEIMDFWMELLLQTYKPTVSTGRCPVLVLEPSKVLQPAILCVSEEDENRTVQLKHITTQKKGLHQWTFPASAIRGVSASKIDERSCFLYVHYNSDDFQLCFPSQLHCNGFCELVNSLLQQAEDSSTQLDHLPEGVIEYVYETDENGDNVVLGKGTYGVVYAGRDLSNQVRIAIKEIPEKDSMYSQPLHEEIALHKRLKHRNIVQYLGSVSQDGFIKIFMEEVPGGSLSSLLRSKWGPLKDNEATIIFYTKQILEGLRYLHANQIVHRDIKGDNVLINTYSGVLKISDFGTSKRLAGINPCTETFTGTLQYMAPEIIDQGPRGYGKPADIWSLGCTIIEMATGKTPFHELGSPQAAMFKVGMFKIHPKVPECMSDEAKAFIMNCFTPNPDDRATASKLLMDAFLRLQPRKKPKAVQEPDPKDFLSIAEYQRSLSVPISIFVEDIDSPSNSIDLSASLDLRWHLSALKTNDISESPPGSSFLPLPEDSPSEMQCSPVSTEESVGLFMLRKDSERRATLHRVLTDYNNLVISNIQESVPQHSEGSSLSADHISELICCLRENIRSPDRMQLTSRLLELRTKLLNAAIPANSLQGVLFSFQDAVKKVLKQQQVKPHWMFALDNLLRQAVQDAITVLLPQLKLQLQSSFEVEDSNPEEQRTSPDTPVVLVPDQLVAPADTQTTPTQENVQTGRPNYPIDLDLDSNCPLSETLRNLRLETLGLLSQLNEQEREYQELLRSSVCRKQEQIDALRTAAAATEDDSLVSHTYSDPEAKALVCWLKSIPVDQDSINKLLSHRFTLDCLLCLASRDDLMYCGIRGGMLCRIWAAITAHRNNQLNKGKD

Target ORF information:

RefSeq Version	XM_011605057.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes mitogen-activated protein kinase kinase kinase 5-like (LOC101071039), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011605057.1

ORF Insert Sequence:

ATGTACACGA CGGAGCGCAG GCGAATGAGC CTGCATGATC TGAAGAGGAG GGATCCGCTT 
CGGGTGTCAG CTGGAAGTTT GTGGCAGGAC TCGCTGGTCA TGGATCCGGG TGCCAACGGC 
GCGGTGAAAC ACGTCGTGTC TCCGAGGAGT CGGACCAGAG CCATATCCGT GGCGTACATC 
GTCAACGAGG ACGCGTCGGT GCCGCAGACT GAGGAGAACC TCTCACTGAC GTGTCTGAAG 
GACGCATGTG CGGACGCGCA CGCCTCTTTA CATACTATTG CATTTGAGAG AATAACCTTG 
GGGACGACAG ACGTCCTGGA TAGTTTCTAC AACGCAGACG TAGCGGTGGC GGAGATGAGC 
GACTCTTTCT GCCAGCCTTC CCTTTTCTAT CACCTCGGAG TGAGAGAGAG TTTCAGCATG 
ACCAACAACA TCATTTTATA CTGTTACAAG CAGGATAGCG ACCTGCAGGC TATCAAGGAG 
CAGTGTGGGA GTTTCACATT CATCCCGTAC GTGGTGTCGC CTCAGGGCAA AGTGTTTGCC 
TGCGATGCTC GCATGATGAC GTGTATGAAA GAGTTGATGC AGCCCAGCTT CCAGCTGGAG 
CCCCTGCTGA CGCCGCTGGT GGAGCGACTG GTGCATCTTC TCAGCAGTGT GCACATTCAA 
TCCAGCGAGT ACTTCAGGGA GTCCATCAGG CAGGAGATCC GAATGGCCCG GGAGCGTTTC 
AGCGGGCAGG ACCTGAGCAA GGAACTGAGA CGCATCCAGA AACGCCTGGA CAGCGTGGAG 
CTGCTCACCC CAGATATCGT CATCAACCTG CTGCTGTCTT ACAGGGACAT TCAGGATTAT 
GATTCCATAA TCAATCTGGT GGAGACTCTG AACAACCTGC CCATGTGTCT GATAGCGCAG 
CATCAGAATA TAAAGTTTCA CTACATATTT GCTCTAAACA GGAGGAATCA CCCCGGAGAC 
CGTGCCAAGG CTTTGCAGTT GATCCTGCCG CTTGTGGAAT CCAGTGACAA GGTGGCGTCG 
GACATCTACT GCCTGTGTGG ACGGATCTAC AAGGACATGT TCATGAGCTC TGGCTTCAGT 
GATCAGAGCA GCAGAGATCA GGCTTGCTGC TGGTATGGCA AAGCTTTTGA AACGGAACCG 
ACTCTTCACT CGGGCATCAA CGTCGTCGTG CTTCTGATGG CAGCCGGCCA TGAATTTGAG 
ACTTCCATCG AGATCCGTAA AATCGGGGTG ACTCTGAACA CTTTGCTGGG GCGGAAGGGC 
AGTTTGGAGA AGATGAAGGA CTACTGGGAT GTCGGCTTCT ACCTCGGAGC GAACATCCTC 
ACTAACGAAC ACAGGAAAGT CATTGAAGCC TGTGAAAAAC TTTACCGGCT GAAGGCTCCC 
GTGTGGTACG TGGCTTCAAT CATGGAGACC TTCATCCTGT TTCGACAGTT TGCCAAACTA 
CCTGAGGTGA AATCGCCCAA ACAGGAGATT ATGGACTTCT GGATGGAGCT GCTTCTGCAG 
ACGTACAAAC CCACAGTTTC CACCGGCCGT TGCCCAGTGC TCGTTCTGGA GCCCAGTAAA 
GTCCTCCAGC CGGCCATTTT GTGCGTGAGC GAGGAGGATG AAAATCGCAC GGTTCAGCTG 
AAACACATCA CAACCCAGAA GAAAGGCTTA CACCAGTGGA CGTTCCCAGC CTCTGCGATT 
CGGGGAGTGA GTGCTTCAAA GATTGATGAG CGGAGTTGCT TCCTGTATGT CCACTACAAC 
TCAGATGACT TCCAGCTCTG TTTCCCCTCT CAGCTTCACT GTAATGGTTT CTGTGAGCTG 
GTGAACTCTC TCCTTCAGCA GGCTGAAGAT TCGTCCACAC AACTGGATCA TCTTCCCGAG 
GGGGTGATCG AGTATGTTTA CGAGACTGAT GAGAATGGTG ACAACGTGGT TCTGGGTAAA 
GGCACGTATG GCGTGGTGTA CGCGGGGAGG GACCTGAGCA ACCAAGTACG CATCGCCATC 
AAGGAAATCC CTGAAAAGGA CAGCATGTAC TCGCAGCCCC TTCATGAAGA AATAGCTCTG 
CATAAGAGGC TAAAACACAG GAACATTGTG CAGTACCTGG GCTCCGTCAG TCAGGATGGA 
TTCATCAAGA TCTTCATGGA GGAAGTACCT GGAGGAAGTT TGTCCTCTCT GCTGCGCTCC 
AAGTGGGGCC CTCTGAAAGA CAATGAAGCA ACCATCATCT TCTACACCAA ACAGATCCTC 
GAGGGGCTCA GATATCTTCA CGCTAATCAA ATTGTCCACC GGGACATCAA GGGCGACAAC 
GTGTTGATCA ACACCTACAG CGGAGTGTTG AAGATCTCCG ACTTTGGGAC CTCCAAACGA 
TTAGCTGGGA TCAACCCCTG CACCGAGACC TTTACTGGAA CTCTGCAATA CATGGCCCCA 
GAGATTATAG ACCAGGGACC TCGGGGTTAT GGGAAACCAG CTGATATCTG GTCTCTGGGG 
TGCACTATTA TTGAAATGGC AACAGGCAAA ACCCCATTCC ATGAACTGGG AAGTCCTCAG 
GCAGCCATGT TTAAGGTGGG CATGTTTAAG ATCCACCCCA AGGTTCCAGA ATGTATGTCA 
GATGAAGCAA AGGCGTTCAT CATGAACTGT TTCACGCCGA ACCCCGATGA CCGAGCCACA 
GCTTCAAAGC TGCTAATGGA TGCCTTCCTC AGGTTACAGC CCAGGAAGAA GCCCAAAGCT 
GTGCAGGAGC CAGACCCCAA AGACTTCCTT AGTATTGCTG AATATCAACG CAGCCTCTCT 
GTCCCGATCT CCATCTTTGT GGAGGACATT GATTCGCCCT CCAATTCGAT CGACCTGTCC 
GCTTCCCTGG ACCTGAGGTG GCACTTGTCA GCCCTCAAGA CGAATGACAT ATCAGAGAGT 
CCGCCTGGCA GCAGCTTCCT GCCACTACCA GAAGACTCTC CATCAGAAAT GCAGTGCAGC 
CCAGTATCAA CTGAGGAGAG TGTTGGTCTG TTTATGCTCA GAAAGGACAG TGAAAGGAGG 
GCCACGCTTC ACCGTGTCCT CACCGACTAC AACAATCTTG TTATTTCAAA TATACAGGAA 
TCTGTGCCTC AGCATAGTGA AGGATCATCT CTAAGTGCAG ACCACATCAG TGAGCTCATT 
TGCTGCTTGC GGGAAAACAT CCGCTCCCCG GACAGGATGC AGCTGACCAG TCGCCTGCTG 
GAACTTCGGA CTAAGCTGCT CAATGCCGCA ATACCGGCAA ATAGCCTCCA GGGGGTGCTA 
TTCAGCTTCC AAGATGCTGT GAAAAAGGTT TTGAAGCAAC AGCAGGTGAA ACCTCACTGG 
ATGTTCGCCC TGGACAATCT ACTGAGACAG GCAGTCCAGG ACGCCATCAC TGTGCTGCTT 
CCACAGCTGA AACTCCAGCT GCAGTCCTCA TTTGAGGTGG AGGACAGTAA TCCAGAAGAG 
CAGCGTACAA GCCCGGACAC TCCAGTTGTT CTTGTTCCTG ACCAGCTGGT GGCGCCAGCA 
GACACACAGA CGACTCCCAC ACAAGAGAAT GTCCAAACAG GAAGGCCCAA CTACCCCATA 
GACCTCGACC TCGACAGCAA TTGCCCCCTC AGTGAGACCT TAAGAAACCT ACGACTGGAG 
ACTTTGGGAC TGCTGAGTCA GTTGAATGAG CAGGAGAGAG AGTACCAGGA GCTACTGAGG 
AGCTCCGTCT GCAGGAAACA GGAACAGATT GATGCACTGA GGACTGCAGC CGCTGCCACC 
GAAGATGACT CATTGGTATC GCACACATAC TCAGATCCTG AAGCCAAGGC TTTGGTGTGC 
TGGCTCAAGT CCATCCCAGT TGATCAAGAC TCAATTAATA AGTTGCTCTC TCACAGGTTC 
ACTTTGGACT GTCTCCTCTG TCTGGCCTCC AGGGATGACT TGATGTATTG TGGAATAAGA 
GGTGGCATGT TGTGTCGAAT TTGGGCAGCC ATCACAGCGC ACAGGAATAA CCAGCTCAAC 
AAAGGGAAGG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101071039 ( XM_011605058.1 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_011603360.1 Takifugu rubripes mitogen-activated protein kinase kinase kinase 5-like (LOC101071039), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OTo25876
Clone ID Related Accession (Same CDS sequence)	XM_011605058.1
Accession Version	XM_011605058.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3945bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1426348800000
Organism	Takifugu rubripes(Fugu rubripes)
Product	mitogen-activated protein kinase kinase kinase 5-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070976.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACACGA CGGAGCGCAG GCGAATGAGC CTGCATGATC TGAAGAGGAG GGATCCGCTT 
CGGGTGTCAG CTGGAAGTTT GTGGCAGGAC TCGCTGGTCA TGGATCCGGG TGCCAACGGC 
GCGGTGAAAC ACGTCGTGTC TCCGAGGAGT CGGACCAGAG CCATATCCGT GGCGTACATC 
GTCAACGAGG ACGCGTCGGT GCCGCAGACT GAGGAGAACC TCTCACTGAC GTGTCTGAAG 
GACGCATGTG CGGACGCGCA CGCCTCTTTA CATACTATTG CATTTGAGAG AATAACCTTG 
GGGACGACAG ACGTCCTGGA TAGTTTCTAC AACGCAGACG TAGCGGTGGC GGAGATGAGC 
GACTCTTTCT GCCAGCCTTC CCTTTTCTAT CACCTCGGAG TGAGAGAGAG TTTCAGCATG 
ACCAACAACA TCATTTTATA CTGTTACAAG CAGGATAGCG ACCTGCAGGC TATCAAGGAG 
CAGTGTGGGA GTTTCACATT CATCCCGTAC GTGGTGTCGC CTCAGGGCAA AGTGTTTGCC 
TGCGATGCTC GCATGATGAC GTGTATGAAA GAGTTGATGC AGCCCAGCTT CCAGCTGGAG 
CCCCTGCTGA CGCCGCTGGT GGAGCGACTG GTGCATCTTC TCAGCAGTGT GCACATTCAA 
TCCAGCGAGT ACTTCAGGGA GTCCATCAGG CAGGAGATCC GAATGGCCCG GGAGCGTTTC 
AGCGGGCAGG ACCTGAGCAA GGAACTGAGA CGCATCCAGA AACGCCTGGA CAGCGTGGAG 
CTGCTCACCC CAGATATCGT CATCAACCTG CTGCTGTCTT ACAGGGACAT TCAGGATTAT 
GATTCCATAA TCAATCTGGT GGAGACTCTG AACAACCTGC CCATGTGTCT GATAGCGCAG 
CATCAGAATA TAAAGTTTCA CTACATATTT GCTCTAAACA GGAGGAATCA CCCCGGAGAC 
CGTGCCAAGG CTTTGCAGTT GATCCTGCCG CTTGTGGAAT CCAGTGACAA GGTGGCGTCG 
GACATCTACT GCCTGTGTGG ACGGATCTAC AAGGACATGT TCATGAGCTC TGGCTTCAGT 
GATCAGAGCA GCAGAGATCA GGCTTGCTGC TGGTATGGCA AAGCTTTTGA AACGGAACCG 
ACTCTTCACT CGGGCATCAA CGTCGTCGTG CTTCTGATGG CAGCCGGCCA TGAATTTGAG 
ACTTCCATCG AGATCCGTAA AATCGGGGTG ACTCTGAACA CTTTGCTGGG GCGGAAGGGC 
AGTTTGGAGA AGATGAAGGA CTACTGGGAT GTCGGCTTCT ACCTCGGAGC GAACATCCTC 
ACTAACGAAC ACAGGAAAGT CATTGAAGCC TGTGAAAAAC TTTACCGGCT GAAGGCTCCC 
GTGTGGTACG TGGCTTCAAT CATGGAGACC TTCATCCTGT TTCGACAGTT TGCCAAACTA 
CCTGAGGTGA AATCGCCCAA ACAGGAGATT ATGGACTTCT GGATGGAGCT GCTTCTGCAG 
ACGTACAAAC CCACAGTTTC CACCGGCCGT TGCCCAGTGC TCGTTCTGGA GCCCAGTAAA 
GTCCTCCAGC CGGCCATTTT GTGCGTGAGC GAGGAGGATG AAAATCGCAC GGTTCAGCTG 
AAACACATCA CAACCCAGAA GAAAGGCTTA CACCAGTGGA CGTTCCCAGC CTCTGCGATT 
CGGGGAGTGA GTGCTTCAAA GATTGATGAG CGGAGTTGCT TCCTGTATGT CCACTACAAC 
TCAGATGACT TCCAGCTCTG TTTCCCCTCT CAGCTTCACT GTAATGGTTT CTGTGAGCTG 
GTGAACTCTC TCCTTCAGCA GGCTGAAGAT TCGTCCACAC AACTGGATCA TCTTCCCGAG 
GGGGTGATCG AGTATGTTTA CGAGACTGAT GAGAATGGTG ACAACGTGGT TCTGGGTAAA 
GGCACGTATG GCGTGGTGTA CGCGGGGAGG GACCTGAGCA ACCAAGTACG CATCGCCATC 
AAGGAAATCC CTGAAAAGGA CAGCATGTAC TCGCAGCCCC TTCATGAAGA AATAGCTCTG 
CATAAGAGGC TAAAACACAG GAACATTGTG CAGTACCTGG GCTCCGTCAG TCAGGATGGA 
TTCATCAAGA TCTTCATGGA GGAAGTACCT GGAGGAAGTT TGTCCTCTCT GCTGCGCTCC 
AAGTGGGGCC CTCTGAAAGA CAATGAAGCA ACCATCATCT TCTACACCAA ACAGATCCTC 
GAGGGGCTCA GATATCTTCA CGCTAATCAA ATTGTCCACC GGGACATCAA GGGCGACAAC 
GTGTTGATCA ACACCTACAG CGGAGTGTTG AAGATCTCCG ACTTTGGGAC CTCCAAACGA 
TTAGCTGGGA TCAACCCCTG CACCGAGACC TTTACTGGAA CTCTGCAATA CATGGCCCCA 
GAGATTATAG ACCAGGGACC TCGGGGTTAT GGGAAACCAG CTGATATCTG GTCTCTGGGG 
TGCACTATTA TTGAAATGGC AACAGGCAAA ACCCCATTCC ATGAACTGGG AAGTCCTCAG 
GCAGCCATGT TTAAGGTGGG CATGTTTAAG ATCCACCCCA AGGTTCCAGA ATGTATGTCA 
GATGAAGCAA AGGCGTTCAT CATGAACTGT TTCACGCCGA ACCCCGATGA CCGAGCCACA 
GCTTCAAAGC TGCTAATGGA TGCCTTCCTC AGGTTACAGC CCAGGAAGAA GCCCAAAGCT 
GTGCAGGAGC CAGACCCCAA AGACTTCCTT AGTATTGCTG AATATCAACG CAGCCTCTCT 
GTCCCGATCT CCATCTTTGT GGAGGACATT GATTCGCCCT CCAATTCGAT CGACCTGTCC 
GCTTCCCTGG ACCTGAGGTG GCACTTGTCA GCCCTCAAGA CGAATGACAT ATCAGAGAGT 
CCGCCTGGCA GCAGCTTCCT GCCACTACCA GAAGACTCTC CATCAGAAAT GCAGTGCAGC 
CCAGTATCAA CTGAGGAGAG TGTTGGTCTG TTTATGCTCA GAAAGGACAG TGAAAGGAGG 
GCCACGCTTC ACCGTGTCCT CACCGACTAC AACAATCTTG TTATTTCAAA TATACAGGAA 
TCTGTGCCTC AGCATAGTGA AGGATCATCT CTAAGTGCAG ACCACATCAG TGAGCTCATT 
TGCTGCTTGC GGGAAAACAT CCGCTCCCCG GACAGGATGC AGCTGACCAG TCGCCTGCTG 
GAACTTCGGA CTAAGCTGCT CAATGCCGCA ATACCGGCAA ATAGCCTCCA GGGGGTGCTA 
TTCAGCTTCC AAGATGCTGT GAAAAAGGTT TTGAAGCAAC AGCAGGTGAA ACCTCACTGG 
ATGTTCGCCC TGGACAATCT ACTGAGACAG GCAGTCCAGG ACGCCATCAC TGTGCTGCTT 
CCACAGCTGA AACTCCAGCT GCAGTCCTCA TTTGAGGTGG AGGACAGTAA TCCAGAAGAG 
CAGCGTACAA GCCCGGACAC TCCAGTTGTT CTTGTTCCTG ACCAGCTGGT GGCGCCAGCA 
GACACACAGA CGACTCCCAC ACAAGAGAAT GTCCAAACAG GAAGGCCCAA CTACCCCATA 
GACCTCGACC TCGACAGCAA TTGCCCCCTC AGTGAGACCT TAAGAAACCT ACGACTGGAG 
ACTTTGGGAC TGCTGAGTCA GTTGAATGAG CAGGAGAGAG AGTACCAGGA GCTACTGAGG 
AGCTCCGTCT GCAGGAAACA GGAACAGATT GATGCACTGA GGACTGCAGC CGCTGCCACC 
GAAGATCCTG AAGCCAAGGC TTTGGTGTGC TGGCTCAAGT CCATCCCAGT TGATCAAGAC 
TCAATTAATA AGTTGCTCTC TCACAGGTTC ACTTTGGACT GTCTCCTCTG TCTGGCCTCC 
AGGGATGACT TGATGTATTG TGGAATAAGA GGTGGCATGT TGTGTCGAAT TTGGGCAGCC 
ATCACAGCGC ACAGGAATAA CCAGCTCAAC AAAGGGAAGG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011603360.1
CDS	177..4121
Translation	MYTTERRRMSLHDLKRRDPLRVSAGSLWQDSLVMDPGANGAVKHVVSPRSRTRAISVAYIVNEDASVPQTEENLSLTCLKDACADAHASLHTIAFERITLGTTDVLDSFYNADVAVAEMSDSFCQPSLFYHLGVRESFSMTNNIILYCYKQDSDLQAIKEQCGSFTFIPYVVSPQGKVFACDARMMTCMKELMQPSFQLEPLLTPLVERLVHLLSSVHIQSSEYFRESIRQEIRMARERFSGQDLSKELRRIQKRLDSVELLTPDIVINLLLSYRDIQDYDSIINLVETLNNLPMCLIAQHQNIKFHYIFALNRRNHPGDRAKALQLILPLVESSDKVASDIYCLCGRIYKDMFMSSGFSDQSSRDQACCWYGKAFETEPTLHSGINVVVLLMAAGHEFETSIEIRKIGVTLNTLLGRKGSLEKMKDYWDVGFYLGANILTNEHRKVIEACEKLYRLKAPVWYVASIMETFILFRQFAKLPEVKSPKQEIMDFWMELLLQTYKPTVSTGRCPVLVLEPSKVLQPAILCVSEEDENRTVQLKHITTQKKGLHQWTFPASAIRGVSASKIDERSCFLYVHYNSDDFQLCFPSQLHCNGFCELVNSLLQQAEDSSTQLDHLPEGVIEYVYETDENGDNVVLGKGTYGVVYAGRDLSNQVRIAIKEIPEKDSMYSQPLHEEIALHKRLKHRNIVQYLGSVSQDGFIKIFMEEVPGGSLSSLLRSKWGPLKDNEATIIFYTKQILEGLRYLHANQIVHRDIKGDNVLINTYSGVLKISDFGTSKRLAGINPCTETFTGTLQYMAPEIIDQGPRGYGKPADIWSLGCTIIEMATGKTPFHELGSPQAAMFKVGMFKIHPKVPECMSDEAKAFIMNCFTPNPDDRATASKLLMDAFLRLQPRKKPKAVQEPDPKDFLSIAEYQRSLSVPISIFVEDIDSPSNSIDLSASLDLRWHLSALKTNDISESPPGSSFLPLPEDSPSEMQCSPVSTEESVGLFMLRKDSERRATLHRVLTDYNNLVISNIQESVPQHSEGSSLSADHISELICCLRENIRSPDRMQLTSRLLELRTKLLNAAIPANSLQGVLFSFQDAVKKVLKQQQVKPHWMFALDNLLRQAVQDAITVLLPQLKLQLQSSFEVEDSNPEEQRTSPDTPVVLVPDQLVAPADTQTTPTQENVQTGRPNYPIDLDLDSNCPLSETLRNLRLETLGLLSQLNEQEREYQELLRSSVCRKQEQIDALRTAAAATEDPEAKALVCWLKSIPVDQDSINKLLSHRFTLDCLLCLASRDDLMYCGIRGGMLCRIWAAITAHRNNQLNKGKD

Target ORF information:

RefSeq Version	XM_011605058.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes mitogen-activated protein kinase kinase kinase 5-like (LOC101071039), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011605058.1

ORF Insert Sequence:

ATGTACACGA CGGAGCGCAG GCGAATGAGC CTGCATGATC TGAAGAGGAG GGATCCGCTT 
CGGGTGTCAG CTGGAAGTTT GTGGCAGGAC TCGCTGGTCA TGGATCCGGG TGCCAACGGC 
GCGGTGAAAC ACGTCGTGTC TCCGAGGAGT CGGACCAGAG CCATATCCGT GGCGTACATC 
GTCAACGAGG ACGCGTCGGT GCCGCAGACT GAGGAGAACC TCTCACTGAC GTGTCTGAAG 
GACGCATGTG CGGACGCGCA CGCCTCTTTA CATACTATTG CATTTGAGAG AATAACCTTG 
GGGACGACAG ACGTCCTGGA TAGTTTCTAC AACGCAGACG TAGCGGTGGC GGAGATGAGC 
GACTCTTTCT GCCAGCCTTC CCTTTTCTAT CACCTCGGAG TGAGAGAGAG TTTCAGCATG 
ACCAACAACA TCATTTTATA CTGTTACAAG CAGGATAGCG ACCTGCAGGC TATCAAGGAG 
CAGTGTGGGA GTTTCACATT CATCCCGTAC GTGGTGTCGC CTCAGGGCAA AGTGTTTGCC 
TGCGATGCTC GCATGATGAC GTGTATGAAA GAGTTGATGC AGCCCAGCTT CCAGCTGGAG 
CCCCTGCTGA CGCCGCTGGT GGAGCGACTG GTGCATCTTC TCAGCAGTGT GCACATTCAA 
TCCAGCGAGT ACTTCAGGGA GTCCATCAGG CAGGAGATCC GAATGGCCCG GGAGCGTTTC 
AGCGGGCAGG ACCTGAGCAA GGAACTGAGA CGCATCCAGA AACGCCTGGA CAGCGTGGAG 
CTGCTCACCC CAGATATCGT CATCAACCTG CTGCTGTCTT ACAGGGACAT TCAGGATTAT 
GATTCCATAA TCAATCTGGT GGAGACTCTG AACAACCTGC CCATGTGTCT GATAGCGCAG 
CATCAGAATA TAAAGTTTCA CTACATATTT GCTCTAAACA GGAGGAATCA CCCCGGAGAC 
CGTGCCAAGG CTTTGCAGTT GATCCTGCCG CTTGTGGAAT CCAGTGACAA GGTGGCGTCG 
GACATCTACT GCCTGTGTGG ACGGATCTAC AAGGACATGT TCATGAGCTC TGGCTTCAGT 
GATCAGAGCA GCAGAGATCA GGCTTGCTGC TGGTATGGCA AAGCTTTTGA AACGGAACCG 
ACTCTTCACT CGGGCATCAA CGTCGTCGTG CTTCTGATGG CAGCCGGCCA TGAATTTGAG 
ACTTCCATCG AGATCCGTAA AATCGGGGTG ACTCTGAACA CTTTGCTGGG GCGGAAGGGC 
AGTTTGGAGA AGATGAAGGA CTACTGGGAT GTCGGCTTCT ACCTCGGAGC GAACATCCTC 
ACTAACGAAC ACAGGAAAGT CATTGAAGCC TGTGAAAAAC TTTACCGGCT GAAGGCTCCC 
GTGTGGTACG TGGCTTCAAT CATGGAGACC TTCATCCTGT TTCGACAGTT TGCCAAACTA 
CCTGAGGTGA AATCGCCCAA ACAGGAGATT ATGGACTTCT GGATGGAGCT GCTTCTGCAG 
ACGTACAAAC CCACAGTTTC CACCGGCCGT TGCCCAGTGC TCGTTCTGGA GCCCAGTAAA 
GTCCTCCAGC CGGCCATTTT GTGCGTGAGC GAGGAGGATG AAAATCGCAC GGTTCAGCTG 
AAACACATCA CAACCCAGAA GAAAGGCTTA CACCAGTGGA CGTTCCCAGC CTCTGCGATT 
CGGGGAGTGA GTGCTTCAAA GATTGATGAG CGGAGTTGCT TCCTGTATGT CCACTACAAC 
TCAGATGACT TCCAGCTCTG TTTCCCCTCT CAGCTTCACT GTAATGGTTT CTGTGAGCTG 
GTGAACTCTC TCCTTCAGCA GGCTGAAGAT TCGTCCACAC AACTGGATCA TCTTCCCGAG 
GGGGTGATCG AGTATGTTTA CGAGACTGAT GAGAATGGTG ACAACGTGGT TCTGGGTAAA 
GGCACGTATG GCGTGGTGTA CGCGGGGAGG GACCTGAGCA ACCAAGTACG CATCGCCATC 
AAGGAAATCC CTGAAAAGGA CAGCATGTAC TCGCAGCCCC TTCATGAAGA AATAGCTCTG 
CATAAGAGGC TAAAACACAG GAACATTGTG CAGTACCTGG GCTCCGTCAG TCAGGATGGA 
TTCATCAAGA TCTTCATGGA GGAAGTACCT GGAGGAAGTT TGTCCTCTCT GCTGCGCTCC 
AAGTGGGGCC CTCTGAAAGA CAATGAAGCA ACCATCATCT TCTACACCAA ACAGATCCTC 
GAGGGGCTCA GATATCTTCA CGCTAATCAA ATTGTCCACC GGGACATCAA GGGCGACAAC 
GTGTTGATCA ACACCTACAG CGGAGTGTTG AAGATCTCCG ACTTTGGGAC CTCCAAACGA 
TTAGCTGGGA TCAACCCCTG CACCGAGACC TTTACTGGAA CTCTGCAATA CATGGCCCCA 
GAGATTATAG ACCAGGGACC TCGGGGTTAT GGGAAACCAG CTGATATCTG GTCTCTGGGG 
TGCACTATTA TTGAAATGGC AACAGGCAAA ACCCCATTCC ATGAACTGGG AAGTCCTCAG 
GCAGCCATGT TTAAGGTGGG CATGTTTAAG ATCCACCCCA AGGTTCCAGA ATGTATGTCA 
GATGAAGCAA AGGCGTTCAT CATGAACTGT TTCACGCCGA ACCCCGATGA CCGAGCCACA 
GCTTCAAAGC TGCTAATGGA TGCCTTCCTC AGGTTACAGC CCAGGAAGAA GCCCAAAGCT 
GTGCAGGAGC CAGACCCCAA AGACTTCCTT AGTATTGCTG AATATCAACG CAGCCTCTCT 
GTCCCGATCT CCATCTTTGT GGAGGACATT GATTCGCCCT CCAATTCGAT CGACCTGTCC 
GCTTCCCTGG ACCTGAGGTG GCACTTGTCA GCCCTCAAGA CGAATGACAT ATCAGAGAGT 
CCGCCTGGCA GCAGCTTCCT GCCACTACCA GAAGACTCTC CATCAGAAAT GCAGTGCAGC 
CCAGTATCAA CTGAGGAGAG TGTTGGTCTG TTTATGCTCA GAAAGGACAG TGAAAGGAGG 
GCCACGCTTC ACCGTGTCCT CACCGACTAC AACAATCTTG TTATTTCAAA TATACAGGAA 
TCTGTGCCTC AGCATAGTGA AGGATCATCT CTAAGTGCAG ACCACATCAG TGAGCTCATT 
TGCTGCTTGC GGGAAAACAT CCGCTCCCCG GACAGGATGC AGCTGACCAG TCGCCTGCTG 
GAACTTCGGA CTAAGCTGCT CAATGCCGCA ATACCGGCAA ATAGCCTCCA GGGGGTGCTA 
TTCAGCTTCC AAGATGCTGT GAAAAAGGTT TTGAAGCAAC AGCAGGTGAA ACCTCACTGG 
ATGTTCGCCC TGGACAATCT ACTGAGACAG GCAGTCCAGG ACGCCATCAC TGTGCTGCTT 
CCACAGCTGA AACTCCAGCT GCAGTCCTCA TTTGAGGTGG AGGACAGTAA TCCAGAAGAG 
CAGCGTACAA GCCCGGACAC TCCAGTTGTT CTTGTTCCTG ACCAGCTGGT GGCGCCAGCA 
GACACACAGA CGACTCCCAC ACAAGAGAAT GTCCAAACAG GAAGGCCCAA CTACCCCATA 
GACCTCGACC TCGACAGCAA TTGCCCCCTC AGTGAGACCT TAAGAAACCT ACGACTGGAG 
ACTTTGGGAC TGCTGAGTCA GTTGAATGAG CAGGAGAGAG AGTACCAGGA GCTACTGAGG 
AGCTCCGTCT GCAGGAAACA GGAACAGATT GATGCACTGA GGACTGCAGC CGCTGCCACC 
GAAGATCCTG AAGCCAAGGC TTTGGTGTGC TGGCTCAAGT CCATCCCAGT TGATCAAGAC 
TCAATTAATA AGTTGCTCTC TCACAGGTTC ACTTTGGACT GTCTCCTCTG TCTGGCCTCC 
AGGGATGACT TGATGTATTG TGGAATAAGA GGTGGCATGT TGTGTCGAAT TTGGGCAGCC 
ATCACAGCGC ACAGGAATAA CCAGCTCAAC AAAGGGAAGG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101071039 ( XM_011605057.2 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_011603359.2 Takifugu rubripes mitogen-activated protein kinase kinase kinase 5-like (LOC101071039), transcript variant X1, mRNA.

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CloneID	OTo69903
Clone ID Related Accession (Same CDS sequence)	XM_011605057.2
Accession Version	XM_011605057.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3975bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562169600000
Organism	Takifugu rubripes(torafugu)
Product	mitogen-activated protein kinase kinase kinase 5-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042291.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_011605057.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACACGA CGGAGCGCAG GCGAATGAGC CTGCATGATC TGAAGAGGAG GGATCCGCTT 
CGGGTGTCAG CTGGAAGTTT GTGGCAGGAC TCGCTGGTCA TGGATCCGGG TGCCAACGGC 
GCGGTGAAAC ACGTCGTGTC TCCGAGGAGT CGGACCAGAG CCATATCCGT GGCGTACATC 
GTCAACGAGG ACGCGTCGGT GCCGCAGACT GAGGAGAACC TCTCACTGAC GTGTCTGAAG 
GACGCATGTG CGGACGCGCA CGCCTCTTTA CATACTATTG CATTTGAGAG AATAACCTTG 
GGGACGACAG ACGTCCTGGA TAGTTTCTAC AACGCAGACG TAGCGGTGGC GGAGATGAGC 
GACTCTTTCT GCCAGCCTTC CCTTTTCTAT CACCTCGGAG TGAGAGAGAG TTTCAGCATG 
ACCAACAACA TCATTTTATA CTGTTACAAG CAGGATAGCG ACCTGCAGGC TATCAAGGAG 
CAGTGTGGGA GTTTCACATT CATCCCGTAC GTGGTGTCGC CTCAGGGCAA AGTGTTTGCC 
TGCGATGCTC GCATGATGAC GTGTATGAAA GAGTTGATGC AGCCCAGCTT CCAGCTGGAG 
CCCCTGCTGA CGCCGCTGGT GGAGCGACTG GTGCATCTTC TCAGCAGTGT GCACATTCAA 
TCCAGCGAGT ACTTCAGGGA GTCCATCAGG CAGGAGATCC GAATGGCCCG GGAGCGTTTC 
AGCGGGCAGG ACCTGAGCAA GGAACTGAGA CGCATCCAGA AACGCCTGGA CAGCGTGGAG 
CTGCTCACCC CAGATATCGT CATCAACCTG CTGCTGTCTT ACAGGGACAT TCAGGATTAT 
GATTCCATAA TCAATCTGGT GGAGACTCTG AACAACCTGC CCATGTGTCT GATAGCGCAG 
CATCAGAATA TAAAGTTTCA CTACATATTT GCTCTAAACA GGAGGAATCA CCCCGGAGAC 
CGTGCCAAGG CTTTGCAGTT GATCCTGCCG CTTGTGGAAT CCAGTGACAA GGTGGCGTCG 
GACATCTACT GCCTGTGTGG ACGGATCTAC AAGGACATGT TCATGAGCTC TGGCTTCAGT 
GATCAGAGCA GCAGAGATCA GGCTTGCTGC TGGTATGGCA AAGCTTTTGA AACGGAACCG 
ACTCTTCACT CGGGCATCAA CGTCGTCGTG CTTCTGATGG CAGCCGGCCA TGAATTTGAG 
ACTTCCATCG AGATCCGTAA AATCGGGGTG ACTCTGAACA CTTTGCTGGG GCGGAAGGGC 
AGTTTGGAGA AGATGAAGGA CTACTGGGAT GTCGGCTTCT ACCTCGGAGC GAACATCCTC 
ACTAACGAAC ACAGGAAAGT CATTGAAGCC TGTGAAAAAC TTTACCGGCT GAAGGCTCCC 
GTGTGGTACG TGGCTTCAAT CATGGAGACC TTCATCCTGT TTCGACAGTT TGCCAAACTA 
CCTGAGGTGA AATCGCCCAA ACAGGAGATT ATGGACTTCT GGATGGAGCT GCTTCTGCAG 
ACGTACAAAC CCACAGTTTC CACCGGCCGT TGCCCAGTGC TCGTTCTGGA GCCCAGTAAA 
GTCCTCCAGC CGGCCATTTT GTGCGTGAGC GAGGAGGATG AAAATCGCAC GGTTCAGCTG 
AAACACATCA CAACCCAGAA GAAAGGCTTA CACCAGTGGA CGTTCCCAGC CTCTGCGATT 
CGGGGAGTGA GTGCTTCAAA GATTGATGAG CGGAGTTGCT TCCTGTATGT CCACTACAAC 
TCAGATGACT TCCAGCTCTG TTTCCCCTCT CAGCTTCACT GTAATGGTTT CTGTGAGCTG 
GTGAACTCTC TCCTTCAGCA GGCTGAAGAT TCGTCCACAC AACTGGATCA TCTTCCCGAG 
GGGGTGATCG AGTATGTTTA CGAGACTGAT GAGAATGGTG ACAACGTGGT TCTGGGTAAA 
GGCACGTATG GCGTGGTGTA CGCGGGGAGG GACCTGAGCA ACCAAGTACG CATCGCCATC 
AAGGAAATCC CTGAAAAGGA CAGCATGTAC TCGCAGCCCC TTCATGAAGA AATAGCTCTG 
CATAAGAGGC TAAAACACAG GAACATTGTG CAGTACCTGG GCTCCGTCAG TCAGGATGGA 
TTCATCAAGA TCTTCATGGA GGAAGTACCT GGAGGAAGTT TGTCCTCTCT GCTGCGCTCC 
AAGTGGGGCC CTCTGAAAGA CAATGAAGCA ACCATCATCT TCTACACCAA ACAGATCCTC 
GAGGGGCTCA GATATCTTCA CGCTAATCAA ATTGTCCACC GGGACATCAA GGGCGACAAC 
GTGTTGATCA ACACCTACAG CGGAGTGTTG AAGATCTCCG ACTTTGGGAC CTCCAAACGA 
TTAGCTGGGA TCAACCCCTG CACCGAGACC TTTACTGGAA CTCTGCAATA CATGGCCCCA 
GAGATTATAG ACCAGGGACC TCGGGGTTAT GGGAAACCAG CTGATATCTG GTCTCTGGGG 
TGCACTATTA TTGAAATGGC AACAGGCAAA ACCCCATTCC ATGAACTGGG AAGTCCTCAG 
GCAGCCATGT TTAAGGTGGG CATGTTTAAG ATCCACCCCA AGGTTCCAGA ATGTATGTCA 
GATGAAGCAA AGGCGTTCAT CATGAACTGT TTCACGCCGA ACCCCGATGA CCGAGCCACA 
GCTTCAAAGC TGCTAATGGA TGCCTTCCTC AGGTCACAGC CCAGGAAGAA GCCCAAAGCT 
GTGCAGGAGC CAGACCCCAA AGACTTCCTT AGTATTGCTG AATATCAACG CAGCCTCTCT 
GTCCCGATCT CCATCTTTGT GGAGGACATT GATTCGCCCT CCAATTCGAT CGACCTGTCC 
GCTTCCCTGG ACCTGAGGTG GCACTTGTCA GCCCTCAAGA CGAATGACAT ATCAGAGAGT 
CCGCCTGGCA GCAGCTTCCT GCCACTACCA GAAGACTCTC CATCAGAAAT GCAGTGCAGC 
CCAGTATCAA CTGAGGAGAG TGTTGGTCTG TTTATGCTCA GAAAGGACAG TGAAAGGAGG 
GCCACGCTTC ACCGTGTCCT CACCGACTAC AACAATCTTG TTATTTCAAA TATACAGGAA 
TCTGTGCCTC AGCATAGTGA AGGATCATCT CTAAGTGCAG ACCACATCAG TGAGCTCATT 
TGCTGCTTGC GGGAAAACAT CCGCTCCCCG GACAGGATGC AGCTGACCAG TCGCCTGCTG 
GAACTTCGGA CTAAGCTGCT CAATGCCGCA ATACCGGCAA ATAGCCTCCA GGGGGTGCTA 
TTCAGCTTCC AAGATGCTGT GAAAAAGGTT TTGAAGCAAC AGCAGGTGAA ACCTCACTGG 
ATGTTCGCCC TGGACAATCT ACTGAGACAG GCAGTCCAGG ACGCCATCAC TGTGCTGCTT 
CCACAGCTGA AACTCCAGCT GCAGTCCTCA TTTGAGGTGG AGGACAGTAA TCCAGAAGAG 
CAGCGTACAA GCCCGGACAC TCCAGTTGTT CTTGTTCCTG ACCAGCTGGT GGCGCCAGCA 
GACACACAGA CGACTCCCAC ACAAGAGAAT GTCCAAACAG GAAGGCCCAA CTACCCCATA 
GACCTCGACC TCGACAGCAA TTGCCCCCTC AGTGAGACCT TAAGAAACCT ACGACTGGAG 
ACTTTGGGAC TGCTGAGTCA GTTGAATGAG CAGGAGAGAG AGTACCAGGA GCTACTGAGG 
AGCTCCGTCT GCAGGAAACA GGAACAGATT GATGCACTGA GGACTGCAGC CGCTGCCACC 
GAAGATGACT CATTGGTATC GCACACATAC TCAGATCCTG AAGCCAAGGC TTTGGTGTGC 
TGGCTCAAGT CCATCCCAGT TGATCAAGAC TCAGTTAATA AGTTGCTCTC TCACAGGTTC 
ACTTTGGACT GTCTCCTCTG TCTGGCCTCC AGGGATGACT TGATGTATTG TGGAATAAGA 
GGTGGCATGT TGTGTCGAAT TTGGGCAGCC ATCACAGCGC ACAGGAATAA CCAGCTCAAC 
AAAGGGAAGG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011603359.2
CDS	236..4210
Translation	MYTTERRRMSLHDLKRRDPLRVSAGSLWQDSLVMDPGANGAVKHVVSPRSRTRAISVAYIVNEDASVPQTEENLSLTCLKDACADAHASLHTIAFERITLGTTDVLDSFYNADVAVAEMSDSFCQPSLFYHLGVRESFSMTNNIILYCYKQDSDLQAIKEQCGSFTFIPYVVSPQGKVFACDARMMTCMKELMQPSFQLEPLLTPLVERLVHLLSSVHIQSSEYFRESIRQEIRMARERFSGQDLSKELRRIQKRLDSVELLTPDIVINLLLSYRDIQDYDSIINLVETLNNLPMCLIAQHQNIKFHYIFALNRRNHPGDRAKALQLILPLVESSDKVASDIYCLCGRIYKDMFMSSGFSDQSSRDQACCWYGKAFETEPTLHSGINVVVLLMAAGHEFETSIEIRKIGVTLNTLLGRKGSLEKMKDYWDVGFYLGANILTNEHRKVIEACEKLYRLKAPVWYVASIMETFILFRQFAKLPEVKSPKQEIMDFWMELLLQTYKPTVSTGRCPVLVLEPSKVLQPAILCVSEEDENRTVQLKHITTQKKGLHQWTFPASAIRGVSASKIDERSCFLYVHYNSDDFQLCFPSQLHCNGFCELVNSLLQQAEDSSTQLDHLPEGVIEYVYETDENGDNVVLGKGTYGVVYAGRDLSNQVRIAIKEIPEKDSMYSQPLHEEIALHKRLKHRNIVQYLGSVSQDGFIKIFMEEVPGGSLSSLLRSKWGPLKDNEATIIFYTKQILEGLRYLHANQIVHRDIKGDNVLINTYSGVLKISDFGTSKRLAGINPCTETFTGTLQYMAPEIIDQGPRGYGKPADIWSLGCTIIEMATGKTPFHELGSPQAAMFKVGMFKIHPKVPECMSDEAKAFIMNCFTPNPDDRATASKLLMDAFLRSQPRKKPKAVQEPDPKDFLSIAEYQRSLSVPISIFVEDIDSPSNSIDLSASLDLRWHLSALKTNDISESPPGSSFLPLPEDSPSEMQCSPVSTEESVGLFMLRKDSERRATLHRVLTDYNNLVISNIQESVPQHSEGSSLSADHISELICCLRENIRSPDRMQLTSRLLELRTKLLNAAIPANSLQGVLFSFQDAVKKVLKQQQVKPHWMFALDNLLRQAVQDAITVLLPQLKLQLQSSFEVEDSNPEEQRTSPDTPVVLVPDQLVAPADTQTTPTQENVQTGRPNYPIDLDLDSNCPLSETLRNLRLETLGLLSQLNEQEREYQELLRSSVCRKQEQIDALRTAAAATEDDSLVSHTYSDPEAKALVCWLKSIPVDQDSVNKLLSHRFTLDCLLCLASRDDLMYCGIRGGMLCRIWAAITAHRNNQLNKGKD

Target ORF information:

RefSeq Version	XM_011605057.2
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes mitogen-activated protein kinase kinase kinase 5-like (LOC101071039), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011605057.2

ORF Insert Sequence:

ATGTACACGA CGGAGCGCAG GCGAATGAGC CTGCATGATC TGAAGAGGAG GGATCCGCTT 
CGGGTGTCAG CTGGAAGTTT GTGGCAGGAC TCGCTGGTCA TGGATCCGGG TGCCAACGGC 
GCGGTGAAAC ACGTCGTGTC TCCGAGGAGT CGGACCAGAG CCATATCCGT GGCGTACATC 
GTCAACGAGG ACGCGTCGGT GCCGCAGACT GAGGAGAACC TCTCACTGAC GTGTCTGAAG 
GACGCATGTG CGGACGCGCA CGCCTCTTTA CATACTATTG CATTTGAGAG AATAACCTTG 
GGGACGACAG ACGTCCTGGA TAGTTTCTAC AACGCAGACG TAGCGGTGGC GGAGATGAGC 
GACTCTTTCT GCCAGCCTTC CCTTTTCTAT CACCTCGGAG TGAGAGAGAG TTTCAGCATG 
ACCAACAACA TCATTTTATA CTGTTACAAG CAGGATAGCG ACCTGCAGGC TATCAAGGAG 
CAGTGTGGGA GTTTCACATT CATCCCGTAC GTGGTGTCGC CTCAGGGCAA AGTGTTTGCC 
TGCGATGCTC GCATGATGAC GTGTATGAAA GAGTTGATGC AGCCCAGCTT CCAGCTGGAG 
CCCCTGCTGA CGCCGCTGGT GGAGCGACTG GTGCATCTTC TCAGCAGTGT GCACATTCAA 
TCCAGCGAGT ACTTCAGGGA GTCCATCAGG CAGGAGATCC GAATGGCCCG GGAGCGTTTC 
AGCGGGCAGG ACCTGAGCAA GGAACTGAGA CGCATCCAGA AACGCCTGGA CAGCGTGGAG 
CTGCTCACCC CAGATATCGT CATCAACCTG CTGCTGTCTT ACAGGGACAT TCAGGATTAT 
GATTCCATAA TCAATCTGGT GGAGACTCTG AACAACCTGC CCATGTGTCT GATAGCGCAG 
CATCAGAATA TAAAGTTTCA CTACATATTT GCTCTAAACA GGAGGAATCA CCCCGGAGAC 
CGTGCCAAGG CTTTGCAGTT GATCCTGCCG CTTGTGGAAT CCAGTGACAA GGTGGCGTCG 
GACATCTACT GCCTGTGTGG ACGGATCTAC AAGGACATGT TCATGAGCTC TGGCTTCAGT 
GATCAGAGCA GCAGAGATCA GGCTTGCTGC TGGTATGGCA AAGCTTTTGA AACGGAACCG 
ACTCTTCACT CGGGCATCAA CGTCGTCGTG CTTCTGATGG CAGCCGGCCA TGAATTTGAG 
ACTTCCATCG AGATCCGTAA AATCGGGGTG ACTCTGAACA CTTTGCTGGG GCGGAAGGGC 
AGTTTGGAGA AGATGAAGGA CTACTGGGAT GTCGGCTTCT ACCTCGGAGC GAACATCCTC 
ACTAACGAAC ACAGGAAAGT CATTGAAGCC TGTGAAAAAC TTTACCGGCT GAAGGCTCCC 
GTGTGGTACG TGGCTTCAAT CATGGAGACC TTCATCCTGT TTCGACAGTT TGCCAAACTA 
CCTGAGGTGA AATCGCCCAA ACAGGAGATT ATGGACTTCT GGATGGAGCT GCTTCTGCAG 
ACGTACAAAC CCACAGTTTC CACCGGCCGT TGCCCAGTGC TCGTTCTGGA GCCCAGTAAA 
GTCCTCCAGC CGGCCATTTT GTGCGTGAGC GAGGAGGATG AAAATCGCAC GGTTCAGCTG 
AAACACATCA CAACCCAGAA GAAAGGCTTA CACCAGTGGA CGTTCCCAGC CTCTGCGATT 
CGGGGAGTGA GTGCTTCAAA GATTGATGAG CGGAGTTGCT TCCTGTATGT CCACTACAAC 
TCAGATGACT TCCAGCTCTG TTTCCCCTCT CAGCTTCACT GTAATGGTTT CTGTGAGCTG 
GTGAACTCTC TCCTTCAGCA GGCTGAAGAT TCGTCCACAC AACTGGATCA TCTTCCCGAG 
GGGGTGATCG AGTATGTTTA CGAGACTGAT GAGAATGGTG ACAACGTGGT TCTGGGTAAA 
GGCACGTATG GCGTGGTGTA CGCGGGGAGG GACCTGAGCA ACCAAGTACG CATCGCCATC 
AAGGAAATCC CTGAAAAGGA CAGCATGTAC TCGCAGCCCC TTCATGAAGA AATAGCTCTG 
CATAAGAGGC TAAAACACAG GAACATTGTG CAGTACCTGG GCTCCGTCAG TCAGGATGGA 
TTCATCAAGA TCTTCATGGA GGAAGTACCT GGAGGAAGTT TGTCCTCTCT GCTGCGCTCC 
AAGTGGGGCC CTCTGAAAGA CAATGAAGCA ACCATCATCT TCTACACCAA ACAGATCCTC 
GAGGGGCTCA GATATCTTCA CGCTAATCAA ATTGTCCACC GGGACATCAA GGGCGACAAC 
GTGTTGATCA ACACCTACAG CGGAGTGTTG AAGATCTCCG ACTTTGGGAC CTCCAAACGA 
TTAGCTGGGA TCAACCCCTG CACCGAGACC TTTACTGGAA CTCTGCAATA CATGGCCCCA 
GAGATTATAG ACCAGGGACC TCGGGGTTAT GGGAAACCAG CTGATATCTG GTCTCTGGGG 
TGCACTATTA TTGAAATGGC AACAGGCAAA ACCCCATTCC ATGAACTGGG AAGTCCTCAG 
GCAGCCATGT TTAAGGTGGG CATGTTTAAG ATCCACCCCA AGGTTCCAGA ATGTATGTCA 
GATGAAGCAA AGGCGTTCAT CATGAACTGT TTCACGCCGA ACCCCGATGA CCGAGCCACA 
GCTTCAAAGC TGCTAATGGA TGCCTTCCTC AGGTCACAGC CCAGGAAGAA GCCCAAAGCT 
GTGCAGGAGC CAGACCCCAA AGACTTCCTT AGTATTGCTG AATATCAACG CAGCCTCTCT 
GTCCCGATCT CCATCTTTGT GGAGGACATT GATTCGCCCT CCAATTCGAT CGACCTGTCC 
GCTTCCCTGG ACCTGAGGTG GCACTTGTCA GCCCTCAAGA CGAATGACAT ATCAGAGAGT 
CCGCCTGGCA GCAGCTTCCT GCCACTACCA GAAGACTCTC CATCAGAAAT GCAGTGCAGC 
CCAGTATCAA CTGAGGAGAG TGTTGGTCTG TTTATGCTCA GAAAGGACAG TGAAAGGAGG 
GCCACGCTTC ACCGTGTCCT CACCGACTAC AACAATCTTG TTATTTCAAA TATACAGGAA 
TCTGTGCCTC AGCATAGTGA AGGATCATCT CTAAGTGCAG ACCACATCAG TGAGCTCATT 
TGCTGCTTGC GGGAAAACAT CCGCTCCCCG GACAGGATGC AGCTGACCAG TCGCCTGCTG 
GAACTTCGGA CTAAGCTGCT CAATGCCGCA ATACCGGCAA ATAGCCTCCA GGGGGTGCTA 
TTCAGCTTCC AAGATGCTGT GAAAAAGGTT TTGAAGCAAC AGCAGGTGAA ACCTCACTGG 
ATGTTCGCCC TGGACAATCT ACTGAGACAG GCAGTCCAGG ACGCCATCAC TGTGCTGCTT 
CCACAGCTGA AACTCCAGCT GCAGTCCTCA TTTGAGGTGG AGGACAGTAA TCCAGAAGAG 
CAGCGTACAA GCCCGGACAC TCCAGTTGTT CTTGTTCCTG ACCAGCTGGT GGCGCCAGCA 
GACACACAGA CGACTCCCAC ACAAGAGAAT GTCCAAACAG GAAGGCCCAA CTACCCCATA 
GACCTCGACC TCGACAGCAA TTGCCCCCTC AGTGAGACCT TAAGAAACCT ACGACTGGAG 
ACTTTGGGAC TGCTGAGTCA GTTGAATGAG CAGGAGAGAG AGTACCAGGA GCTACTGAGG 
AGCTCCGTCT GCAGGAAACA GGAACAGATT GATGCACTGA GGACTGCAGC CGCTGCCACC 
GAAGATGACT CATTGGTATC GCACACATAC TCAGATCCTG AAGCCAAGGC TTTGGTGTGC 
TGGCTCAAGT CCATCCCAGT TGATCAAGAC TCAGTTAATA AGTTGCTCTC TCACAGGTTC 
ACTTTGGACT GTCTCCTCTG TCTGGCCTCC AGGGATGACT TGATGTATTG TGGAATAAGA 
GGTGGCATGT TGTGTCGAAT TTGGGCAGCC ATCACAGCGC ACAGGAATAA CCAGCTCAAC 
AAAGGGAAGG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101071039 ( XM_011605058.2 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_011603360.2 Takifugu rubripes mitogen-activated protein kinase kinase kinase 5-like (LOC101071039), transcript variant X2, mRNA.

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CloneID	OTo69904
Clone ID Related Accession (Same CDS sequence)	XM_011605058.2
Accession Version	XM_011605058.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3945bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562169600000
Organism	Takifugu rubripes(torafugu)
Product	mitogen-activated protein kinase kinase kinase 5-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042291.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_011605058.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACACGA CGGAGCGCAG GCGAATGAGC CTGCATGATC TGAAGAGGAG GGATCCGCTT 
CGGGTGTCAG CTGGAAGTTT GTGGCAGGAC TCGCTGGTCA TGGATCCGGG TGCCAACGGC 
GCGGTGAAAC ACGTCGTGTC TCCGAGGAGT CGGACCAGAG CCATATCCGT GGCGTACATC 
GTCAACGAGG ACGCGTCGGT GCCGCAGACT GAGGAGAACC TCTCACTGAC GTGTCTGAAG 
GACGCATGTG CGGACGCGCA CGCCTCTTTA CATACTATTG CATTTGAGAG AATAACCTTG 
GGGACGACAG ACGTCCTGGA TAGTTTCTAC AACGCAGACG TAGCGGTGGC GGAGATGAGC 
GACTCTTTCT GCCAGCCTTC CCTTTTCTAT CACCTCGGAG TGAGAGAGAG TTTCAGCATG 
ACCAACAACA TCATTTTATA CTGTTACAAG CAGGATAGCG ACCTGCAGGC TATCAAGGAG 
CAGTGTGGGA GTTTCACATT CATCCCGTAC GTGGTGTCGC CTCAGGGCAA AGTGTTTGCC 
TGCGATGCTC GCATGATGAC GTGTATGAAA GAGTTGATGC AGCCCAGCTT CCAGCTGGAG 
CCCCTGCTGA CGCCGCTGGT GGAGCGACTG GTGCATCTTC TCAGCAGTGT GCACATTCAA 
TCCAGCGAGT ACTTCAGGGA GTCCATCAGG CAGGAGATCC GAATGGCCCG GGAGCGTTTC 
AGCGGGCAGG ACCTGAGCAA GGAACTGAGA CGCATCCAGA AACGCCTGGA CAGCGTGGAG 
CTGCTCACCC CAGATATCGT CATCAACCTG CTGCTGTCTT ACAGGGACAT TCAGGATTAT 
GATTCCATAA TCAATCTGGT GGAGACTCTG AACAACCTGC CCATGTGTCT GATAGCGCAG 
CATCAGAATA TAAAGTTTCA CTACATATTT GCTCTAAACA GGAGGAATCA CCCCGGAGAC 
CGTGCCAAGG CTTTGCAGTT GATCCTGCCG CTTGTGGAAT CCAGTGACAA GGTGGCGTCG 
GACATCTACT GCCTGTGTGG ACGGATCTAC AAGGACATGT TCATGAGCTC TGGCTTCAGT 
GATCAGAGCA GCAGAGATCA GGCTTGCTGC TGGTATGGCA AAGCTTTTGA AACGGAACCG 
ACTCTTCACT CGGGCATCAA CGTCGTCGTG CTTCTGATGG CAGCCGGCCA TGAATTTGAG 
ACTTCCATCG AGATCCGTAA AATCGGGGTG ACTCTGAACA CTTTGCTGGG GCGGAAGGGC 
AGTTTGGAGA AGATGAAGGA CTACTGGGAT GTCGGCTTCT ACCTCGGAGC GAACATCCTC 
ACTAACGAAC ACAGGAAAGT CATTGAAGCC TGTGAAAAAC TTTACCGGCT GAAGGCTCCC 
GTGTGGTACG TGGCTTCAAT CATGGAGACC TTCATCCTGT TTCGACAGTT TGCCAAACTA 
CCTGAGGTGA AATCGCCCAA ACAGGAGATT ATGGACTTCT GGATGGAGCT GCTTCTGCAG 
ACGTACAAAC CCACAGTTTC CACCGGCCGT TGCCCAGTGC TCGTTCTGGA GCCCAGTAAA 
GTCCTCCAGC CGGCCATTTT GTGCGTGAGC GAGGAGGATG AAAATCGCAC GGTTCAGCTG 
AAACACATCA CAACCCAGAA GAAAGGCTTA CACCAGTGGA CGTTCCCAGC CTCTGCGATT 
CGGGGAGTGA GTGCTTCAAA GATTGATGAG CGGAGTTGCT TCCTGTATGT CCACTACAAC 
TCAGATGACT TCCAGCTCTG TTTCCCCTCT CAGCTTCACT GTAATGGTTT CTGTGAGCTG 
GTGAACTCTC TCCTTCAGCA GGCTGAAGAT TCGTCCACAC AACTGGATCA TCTTCCCGAG 
GGGGTGATCG AGTATGTTTA CGAGACTGAT GAGAATGGTG ACAACGTGGT TCTGGGTAAA 
GGCACGTATG GCGTGGTGTA CGCGGGGAGG GACCTGAGCA ACCAAGTACG CATCGCCATC 
AAGGAAATCC CTGAAAAGGA CAGCATGTAC TCGCAGCCCC TTCATGAAGA AATAGCTCTG 
CATAAGAGGC TAAAACACAG GAACATTGTG CAGTACCTGG GCTCCGTCAG TCAGGATGGA 
TTCATCAAGA TCTTCATGGA GGAAGTACCT GGAGGAAGTT TGTCCTCTCT GCTGCGCTCC 
AAGTGGGGCC CTCTGAAAGA CAATGAAGCA ACCATCATCT TCTACACCAA ACAGATCCTC 
GAGGGGCTCA GATATCTTCA CGCTAATCAA ATTGTCCACC GGGACATCAA GGGCGACAAC 
GTGTTGATCA ACACCTACAG CGGAGTGTTG AAGATCTCCG ACTTTGGGAC CTCCAAACGA 
TTAGCTGGGA TCAACCCCTG CACCGAGACC TTTACTGGAA CTCTGCAATA CATGGCCCCA 
GAGATTATAG ACCAGGGACC TCGGGGTTAT GGGAAACCAG CTGATATCTG GTCTCTGGGG 
TGCACTATTA TTGAAATGGC AACAGGCAAA ACCCCATTCC ATGAACTGGG AAGTCCTCAG 
GCAGCCATGT TTAAGGTGGG CATGTTTAAG ATCCACCCCA AGGTTCCAGA ATGTATGTCA 
GATGAAGCAA AGGCGTTCAT CATGAACTGT TTCACGCCGA ACCCCGATGA CCGAGCCACA 
GCTTCAAAGC TGCTAATGGA TGCCTTCCTC AGGTCACAGC CCAGGAAGAA GCCCAAAGCT 
GTGCAGGAGC CAGACCCCAA AGACTTCCTT AGTATTGCTG AATATCAACG CAGCCTCTCT 
GTCCCGATCT CCATCTTTGT GGAGGACATT GATTCGCCCT CCAATTCGAT CGACCTGTCC 
GCTTCCCTGG ACCTGAGGTG GCACTTGTCA GCCCTCAAGA CGAATGACAT ATCAGAGAGT 
CCGCCTGGCA GCAGCTTCCT GCCACTACCA GAAGACTCTC CATCAGAAAT GCAGTGCAGC 
CCAGTATCAA CTGAGGAGAG TGTTGGTCTG TTTATGCTCA GAAAGGACAG TGAAAGGAGG 
GCCACGCTTC ACCGTGTCCT CACCGACTAC AACAATCTTG TTATTTCAAA TATACAGGAA 
TCTGTGCCTC AGCATAGTGA AGGATCATCT CTAAGTGCAG ACCACATCAG TGAGCTCATT 
TGCTGCTTGC GGGAAAACAT CCGCTCCCCG GACAGGATGC AGCTGACCAG TCGCCTGCTG 
GAACTTCGGA CTAAGCTGCT CAATGCCGCA ATACCGGCAA ATAGCCTCCA GGGGGTGCTA 
TTCAGCTTCC AAGATGCTGT GAAAAAGGTT TTGAAGCAAC AGCAGGTGAA ACCTCACTGG 
ATGTTCGCCC TGGACAATCT ACTGAGACAG GCAGTCCAGG ACGCCATCAC TGTGCTGCTT 
CCACAGCTGA AACTCCAGCT GCAGTCCTCA TTTGAGGTGG AGGACAGTAA TCCAGAAGAG 
CAGCGTACAA GCCCGGACAC TCCAGTTGTT CTTGTTCCTG ACCAGCTGGT GGCGCCAGCA 
GACACACAGA CGACTCCCAC ACAAGAGAAT GTCCAAACAG GAAGGCCCAA CTACCCCATA 
GACCTCGACC TCGACAGCAA TTGCCCCCTC AGTGAGACCT TAAGAAACCT ACGACTGGAG 
ACTTTGGGAC TGCTGAGTCA GTTGAATGAG CAGGAGAGAG AGTACCAGGA GCTACTGAGG 
AGCTCCGTCT GCAGGAAACA GGAACAGATT GATGCACTGA GGACTGCAGC CGCTGCCACC 
GAAGATCCTG AAGCCAAGGC TTTGGTGTGC TGGCTCAAGT CCATCCCAGT TGATCAAGAC 
TCAGTTAATA AGTTGCTCTC TCACAGGTTC ACTTTGGACT GTCTCCTCTG TCTGGCCTCC 
AGGGATGACT TGATGTATTG TGGAATAAGA GGTGGCATGT TGTGTCGAAT TTGGGCAGCC 
ATCACAGCGC ACAGGAATAA CCAGCTCAAC AAAGGGAAGG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011603360.2
CDS	563..4507
Translation	MYTTERRRMSLHDLKRRDPLRVSAGSLWQDSLVMDPGANGAVKHVVSPRSRTRAISVAYIVNEDASVPQTEENLSLTCLKDACADAHASLHTIAFERITLGTTDVLDSFYNADVAVAEMSDSFCQPSLFYHLGVRESFSMTNNIILYCYKQDSDLQAIKEQCGSFTFIPYVVSPQGKVFACDARMMTCMKELMQPSFQLEPLLTPLVERLVHLLSSVHIQSSEYFRESIRQEIRMARERFSGQDLSKELRRIQKRLDSVELLTPDIVINLLLSYRDIQDYDSIINLVETLNNLPMCLIAQHQNIKFHYIFALNRRNHPGDRAKALQLILPLVESSDKVASDIYCLCGRIYKDMFMSSGFSDQSSRDQACCWYGKAFETEPTLHSGINVVVLLMAAGHEFETSIEIRKIGVTLNTLLGRKGSLEKMKDYWDVGFYLGANILTNEHRKVIEACEKLYRLKAPVWYVASIMETFILFRQFAKLPEVKSPKQEIMDFWMELLLQTYKPTVSTGRCPVLVLEPSKVLQPAILCVSEEDENRTVQLKHITTQKKGLHQWTFPASAIRGVSASKIDERSCFLYVHYNSDDFQLCFPSQLHCNGFCELVNSLLQQAEDSSTQLDHLPEGVIEYVYETDENGDNVVLGKGTYGVVYAGRDLSNQVRIAIKEIPEKDSMYSQPLHEEIALHKRLKHRNIVQYLGSVSQDGFIKIFMEEVPGGSLSSLLRSKWGPLKDNEATIIFYTKQILEGLRYLHANQIVHRDIKGDNVLINTYSGVLKISDFGTSKRLAGINPCTETFTGTLQYMAPEIIDQGPRGYGKPADIWSLGCTIIEMATGKTPFHELGSPQAAMFKVGMFKIHPKVPECMSDEAKAFIMNCFTPNPDDRATASKLLMDAFLRSQPRKKPKAVQEPDPKDFLSIAEYQRSLSVPISIFVEDIDSPSNSIDLSASLDLRWHLSALKTNDISESPPGSSFLPLPEDSPSEMQCSPVSTEESVGLFMLRKDSERRATLHRVLTDYNNLVISNIQESVPQHSEGSSLSADHISELICCLRENIRSPDRMQLTSRLLELRTKLLNAAIPANSLQGVLFSFQDAVKKVLKQQQVKPHWMFALDNLLRQAVQDAITVLLPQLKLQLQSSFEVEDSNPEEQRTSPDTPVVLVPDQLVAPADTQTTPTQENVQTGRPNYPIDLDLDSNCPLSETLRNLRLETLGLLSQLNEQEREYQELLRSSVCRKQEQIDALRTAAAATEDPEAKALVCWLKSIPVDQDSVNKLLSHRFTLDCLLCLASRDDLMYCGIRGGMLCRIWAAITAHRNNQLNKGKD

Target ORF information:

RefSeq Version	XM_011605058.2
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes mitogen-activated protein kinase kinase kinase 5-like (LOC101071039), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011605058.2

ORF Insert Sequence:

ATGTACACGA CGGAGCGCAG GCGAATGAGC CTGCATGATC TGAAGAGGAG GGATCCGCTT 
CGGGTGTCAG CTGGAAGTTT GTGGCAGGAC TCGCTGGTCA TGGATCCGGG TGCCAACGGC 
GCGGTGAAAC ACGTCGTGTC TCCGAGGAGT CGGACCAGAG CCATATCCGT GGCGTACATC 
GTCAACGAGG ACGCGTCGGT GCCGCAGACT GAGGAGAACC TCTCACTGAC GTGTCTGAAG 
GACGCATGTG CGGACGCGCA CGCCTCTTTA CATACTATTG CATTTGAGAG AATAACCTTG 
GGGACGACAG ACGTCCTGGA TAGTTTCTAC AACGCAGACG TAGCGGTGGC GGAGATGAGC 
GACTCTTTCT GCCAGCCTTC CCTTTTCTAT CACCTCGGAG TGAGAGAGAG TTTCAGCATG 
ACCAACAACA TCATTTTATA CTGTTACAAG CAGGATAGCG ACCTGCAGGC TATCAAGGAG 
CAGTGTGGGA GTTTCACATT CATCCCGTAC GTGGTGTCGC CTCAGGGCAA AGTGTTTGCC 
TGCGATGCTC GCATGATGAC GTGTATGAAA GAGTTGATGC AGCCCAGCTT CCAGCTGGAG 
CCCCTGCTGA CGCCGCTGGT GGAGCGACTG GTGCATCTTC TCAGCAGTGT GCACATTCAA 
TCCAGCGAGT ACTTCAGGGA GTCCATCAGG CAGGAGATCC GAATGGCCCG GGAGCGTTTC 
AGCGGGCAGG ACCTGAGCAA GGAACTGAGA CGCATCCAGA AACGCCTGGA CAGCGTGGAG 
CTGCTCACCC CAGATATCGT CATCAACCTG CTGCTGTCTT ACAGGGACAT TCAGGATTAT 
GATTCCATAA TCAATCTGGT GGAGACTCTG AACAACCTGC CCATGTGTCT GATAGCGCAG 
CATCAGAATA TAAAGTTTCA CTACATATTT GCTCTAAACA GGAGGAATCA CCCCGGAGAC 
CGTGCCAAGG CTTTGCAGTT GATCCTGCCG CTTGTGGAAT CCAGTGACAA GGTGGCGTCG 
GACATCTACT GCCTGTGTGG ACGGATCTAC AAGGACATGT TCATGAGCTC TGGCTTCAGT 
GATCAGAGCA GCAGAGATCA GGCTTGCTGC TGGTATGGCA AAGCTTTTGA AACGGAACCG 
ACTCTTCACT CGGGCATCAA CGTCGTCGTG CTTCTGATGG CAGCCGGCCA TGAATTTGAG 
ACTTCCATCG AGATCCGTAA AATCGGGGTG ACTCTGAACA CTTTGCTGGG GCGGAAGGGC 
AGTTTGGAGA AGATGAAGGA CTACTGGGAT GTCGGCTTCT ACCTCGGAGC GAACATCCTC 
ACTAACGAAC ACAGGAAAGT CATTGAAGCC TGTGAAAAAC TTTACCGGCT GAAGGCTCCC 
GTGTGGTACG TGGCTTCAAT CATGGAGACC TTCATCCTGT TTCGACAGTT TGCCAAACTA 
CCTGAGGTGA AATCGCCCAA ACAGGAGATT ATGGACTTCT GGATGGAGCT GCTTCTGCAG 
ACGTACAAAC CCACAGTTTC CACCGGCCGT TGCCCAGTGC TCGTTCTGGA GCCCAGTAAA 
GTCCTCCAGC CGGCCATTTT GTGCGTGAGC GAGGAGGATG AAAATCGCAC GGTTCAGCTG 
AAACACATCA CAACCCAGAA GAAAGGCTTA CACCAGTGGA CGTTCCCAGC CTCTGCGATT 
CGGGGAGTGA GTGCTTCAAA GATTGATGAG CGGAGTTGCT TCCTGTATGT CCACTACAAC 
TCAGATGACT TCCAGCTCTG TTTCCCCTCT CAGCTTCACT GTAATGGTTT CTGTGAGCTG 
GTGAACTCTC TCCTTCAGCA GGCTGAAGAT TCGTCCACAC AACTGGATCA TCTTCCCGAG 
GGGGTGATCG AGTATGTTTA CGAGACTGAT GAGAATGGTG ACAACGTGGT TCTGGGTAAA 
GGCACGTATG GCGTGGTGTA CGCGGGGAGG GACCTGAGCA ACCAAGTACG CATCGCCATC 
AAGGAAATCC CTGAAAAGGA CAGCATGTAC TCGCAGCCCC TTCATGAAGA AATAGCTCTG 
CATAAGAGGC TAAAACACAG GAACATTGTG CAGTACCTGG GCTCCGTCAG TCAGGATGGA 
TTCATCAAGA TCTTCATGGA GGAAGTACCT GGAGGAAGTT TGTCCTCTCT GCTGCGCTCC 
AAGTGGGGCC CTCTGAAAGA CAATGAAGCA ACCATCATCT TCTACACCAA ACAGATCCTC 
GAGGGGCTCA GATATCTTCA CGCTAATCAA ATTGTCCACC GGGACATCAA GGGCGACAAC 
GTGTTGATCA ACACCTACAG CGGAGTGTTG AAGATCTCCG ACTTTGGGAC CTCCAAACGA 
TTAGCTGGGA TCAACCCCTG CACCGAGACC TTTACTGGAA CTCTGCAATA CATGGCCCCA 
GAGATTATAG ACCAGGGACC TCGGGGTTAT GGGAAACCAG CTGATATCTG GTCTCTGGGG 
TGCACTATTA TTGAAATGGC AACAGGCAAA ACCCCATTCC ATGAACTGGG AAGTCCTCAG 
GCAGCCATGT TTAAGGTGGG CATGTTTAAG ATCCACCCCA AGGTTCCAGA ATGTATGTCA 
GATGAAGCAA AGGCGTTCAT CATGAACTGT TTCACGCCGA ACCCCGATGA CCGAGCCACA 
GCTTCAAAGC TGCTAATGGA TGCCTTCCTC AGGTCACAGC CCAGGAAGAA GCCCAAAGCT 
GTGCAGGAGC CAGACCCCAA AGACTTCCTT AGTATTGCTG AATATCAACG CAGCCTCTCT 
GTCCCGATCT CCATCTTTGT GGAGGACATT GATTCGCCCT CCAATTCGAT CGACCTGTCC 
GCTTCCCTGG ACCTGAGGTG GCACTTGTCA GCCCTCAAGA CGAATGACAT ATCAGAGAGT 
CCGCCTGGCA GCAGCTTCCT GCCACTACCA GAAGACTCTC CATCAGAAAT GCAGTGCAGC 
CCAGTATCAA CTGAGGAGAG TGTTGGTCTG TTTATGCTCA GAAAGGACAG TGAAAGGAGG 
GCCACGCTTC ACCGTGTCCT CACCGACTAC AACAATCTTG TTATTTCAAA TATACAGGAA 
TCTGTGCCTC AGCATAGTGA AGGATCATCT CTAAGTGCAG ACCACATCAG TGAGCTCATT 
TGCTGCTTGC GGGAAAACAT CCGCTCCCCG GACAGGATGC AGCTGACCAG TCGCCTGCTG 
GAACTTCGGA CTAAGCTGCT CAATGCCGCA ATACCGGCAA ATAGCCTCCA GGGGGTGCTA 
TTCAGCTTCC AAGATGCTGT GAAAAAGGTT TTGAAGCAAC AGCAGGTGAA ACCTCACTGG 
ATGTTCGCCC TGGACAATCT ACTGAGACAG GCAGTCCAGG ACGCCATCAC TGTGCTGCTT 
CCACAGCTGA AACTCCAGCT GCAGTCCTCA TTTGAGGTGG AGGACAGTAA TCCAGAAGAG 
CAGCGTACAA GCCCGGACAC TCCAGTTGTT CTTGTTCCTG ACCAGCTGGT GGCGCCAGCA 
GACACACAGA CGACTCCCAC ACAAGAGAAT GTCCAAACAG GAAGGCCCAA CTACCCCATA 
GACCTCGACC TCGACAGCAA TTGCCCCCTC AGTGAGACCT TAAGAAACCT ACGACTGGAG 
ACTTTGGGAC TGCTGAGTCA GTTGAATGAG CAGGAGAGAG AGTACCAGGA GCTACTGAGG 
AGCTCCGTCT GCAGGAAACA GGAACAGATT GATGCACTGA GGACTGCAGC CGCTGCCACC 
GAAGATCCTG AAGCCAAGGC TTTGGTGTGC TGGCTCAAGT CCATCCCAGT TGATCAAGAC 
TCAGTTAATA AGTTGCTCTC TCACAGGTTC ACTTTGGACT GTCTCCTCTG TCTGGCCTCC 
AGGGATGACT TGATGTATTG TGGAATAAGA GGTGGCATGT TGTGTCGAAT TTGGGCAGCC 
ATCACAGCGC ACAGGAATAA CCAGCTCAAC AAAGGGAAGG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC101071039 cDNA ORF clone, Takifugu rubripes(Fugu rubripes)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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