pdzrn3 cDNA ORF clone, Takifugu rubripes(torafugu)

Gene
Clones
gRNAs

Summary

Gene Symbol	pdzrn3
Entrez Gene ID	101064578
Full Name	PDZ domain containing ring finger 3
Gene Type	protein-coding
Organism	Takifugu rubripes(torafugu)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003973731.2	XP_003973780.1	E3 ubiquitin-protein ligase PDZRN3
XM_003973731.3	XP_003973780.2	LOW QUALITY PROTEIN: E3 ubiquitin-protein ligase PDZRN3

Pathways from BioSystems
Gene Ontology

The following pdzrn3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pdzrn3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTo12605	XM_003973731.2 Latest version!	Takifugu rubripes PDZ domain containing ring finger 3 (pdzrn3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OTo55546	XM_003973731.3 Latest version!	Takifugu rubripes PDZ domain containing ring finger 3 (pdzrn3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pdzrn3 ( XM_003973731.2 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_003973780.1 Takifugu rubripes PDZ domain containing ring finger 3 (pdzrn3), mRNA.

Price & Availability↑

CloneID	OTo12605
Clone ID Related Accession (Same CDS sequence)	XM_003973731.2
Accession Version	XM_003973731.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3129bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1426348800000
Organism	Takifugu rubripes(Fugu rubripes)
Product	E3 ubiquitin-protein ligase PDZRN3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004071139.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Mar 16, 2015 this sequence version replaced XM_003973731.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGATTCG AGCTGGACCG CTTCAAGGGG ACCGTGGACC CGGATTTTAA ATGCAACTTG 
TGCAATAAAG TGTTGGAAGA CCCGCTGACT ACTCCGTGCG GTCACGTCTT CTGCTCCGGC 
TGCGTGCTGC CCTGGGTCGT CCAGCAGAGC AGCTGCCCCG TCAAATGTCA GCGGATCTCG 
GCCAAGGAGC TGAACCACGT CCTGCCGCTG AAGAACCTCA TCCTGAAGCT GGAGATCAAA 
TGTGACAATC ACGCGAGGGG CTGTGATGCG GTGGTGAAGC TGCAGCATCT GGCCGAGCAC 
GCCGAGATGT GCGAGTACTC CCCGGTGAAG TGTCGGAACA AGGGATGCAG CGAGGTGCTG 
AACCTGGGGG ACATGGACGC TCACATGCGC GGGACGTGCG AGCACAGGGC GGTGGGGCTG 
TGCGAGGGGG GCTGCGGGCT GATGCTCACC TACAGGGAGC AGAGAGTCAG CGGCCACTGC 
TGCGTCCGAG CCCTGAAGGC GCACAACAGC GCGCTGCAGT GCAAACTCGT CTGCCTGGAC 
AGAGAGTTCA AGAAGCAGAC TATTAAAGCC AACAAGCGGG AGAAGGCGCT GCTGGCTCAA 
CTGTCTGCCG TGCACGGCGA GCTGCAGATG ACAGCTCTGA AATATCAGAA GAAATTCACC 
GAGTACAGCG AGAGGATCGA CTCCCTCACC AAGACGGTGG CTTTCTCCTG CAAGGAAGGT 
GAAGCTAAAA GTGTGACCGT TGTCCTTCTG CGTGAGGGCG GATCTCTGGG GTTTAACATC 
GTGGGAGGAA GGCCATGCTC GGACGACATT GACAGCATTT CAAACGAAGG CATCTTTGTG 
TCCAAGATTG TGGAGAAAGG TCCTGCTGAC AAAGACGGAG GCCTCCAGCT CCACGACAAA 
ATATTGGAGG TCAATGGCAA AGAGCTCTCC AAGGCCACCC ATGACCAGGC GGTGGAGGCT 
TTCCGCACGG CCAAGGAGCC CATCGTGGTC CAGGTTCTGC GCCGAGGCCC GGCTGCTGAC 
TCCCAGGTCT CGGACGTCGG TACCCAGACT GACATCACCC TTCAGCACAT CACGGCTCTC 
ACAGACCTGC CTCCCTCCTC ACCCCCAATA CCGGAGCTGG AGGAGTATCT GCTCCCTGAG 
GAGCATCCTT CAGGACATGC CTACTTTGAC CCTACGGACT TCCTGGAGGG CATCCAGCAG 
GACATCGAGC GTGAGGAGAT GGAATATGAG GAGGTGGATT TGTACAGAGA CAACATACAG 
GAGAAGCTGG GCCTGACCAT CTGCTACAGA ACTGATGATG AAGATGAAGC TGGGATCTAC 
ATTAGTGAGA TTGATCCCAA CAGCATTGCA GCCAAGGACG GCAGAATTAG AGAAGGTGAC 
AAGATCATCC AGATCAACGG TGTAGAGATC CAGAACAGAG AAGATGCTGT GGCCCTGCTG 
ACCAGAGAGG GGAACCAGAA CATCTCCCTG CTGGTGGCCC GGCCAGAGAT CCAGCTGGAT 
GAAGGCTGGA TGGATGATGA CAGGAACGAC TTTCTGGATG ACCTCCACAT GGACATGTTG 
GAGCAGCAGC ACCATCAGGC CATGCAGTTC ACTGCCAGCA TGCTGCAGCA GAAGAAGCAT 
GAAGAGGATG GAGGCACCAC GGACACGGCC ACGCTCCTGT CCAACCACCA CGAGAAGGAC 
AGCGGGGTGG GCCGCACCGA CGAGAGCACG CGGAACGACG AGAGCTCCGA GCAGGAGAAC 
CTCGGCGACG ACCAGACCAC GGCCTCCAAC ACGCTCGGCA GCTGCCGGAA GCTGAACTAC 
AGCCAGGACA CCCTCGGCAG CGCCGACCTG CCCTTCAGCG GCGAGTCGTT CATCTCCGCG 
GACTACGCAG ACGCCGACTT CCTGGGAATC ACGGCTGACG AGTGCCAGCG CTTTCGGGAG 
CTCCTGGAAC TGAAGTGTCA GATGAGGAAC GGCGGAGCTC AGGGCCTCCA CTGCCAAGGC 
GCTGAAGGTC AGGACCAGGA AGGTGTGGAC AAAGAGCTAG AGCTGCTCAA TGAGGAGCTA 
CGGACCATCG AGCTGGAGTG CCTGAACATC GTCCGCGCTC ACAAGATGCA GCTGCTGAAG 
GAGCAGTGCC GGGAATCCTG GATGCTCCAC GACAGCGGCT TCCGCAACTA CAACACCAGC 
ATTGACGCAC GCCGCCACGA GCTCTCAGAC ATCACAGAGC TGCCCGAAAA GTCTGACAAA 
GACAGCTCGA GCGCCTACAA CACCGGGGAG AGCTGCCGCA GCACCCCCCT CACGCTGGAG 
CTGTCTCCAG ACAACTCGCT TCGTAGAGGG ACGGAGAACG CGGCTCCGAC CGGAGCGCCG 
GCCGCCGGCA GCTCCGGTGG GAGGGCGCTC GAGCCTCTTT TATTCCCTGG CCAAGATCAA 
GCATCAGGCC CCAGCCGGAG CAAAGGCTCC TCCAAGGATC CGGCTGGAGG TCTGCAAGCC 
GAAAGCAAGG AAAGGAAGCC GGGCGAGTCC AGCAAGTCCG GGCGGAGCTT TTCCCAGCCG 
CACTCTCCAT ACAAACACGC CCACATCCCC GCCCACGCCC AGCACTACCA GAGCTACATG 
CAGCTGATCC AGCAGAAATC GGCTGTGGAG TACGCCCAGA GCCAGATGAG CCTGGTTAGC 
ATGTGCCGCG ACCCCATCAG CCCCACCGAC CTCGAGCCTA AGATGGAGTG GAAGGTGAAG 
GTGCGCAGCG ACGGCACCCG CTACATCACC AAGCGGCCCA TCCGGGACAA GCTGCTGCGG 
GAGCGCGCCC TGCGCATTCA CGAGGAGCGC AGCGGCATGA CCACGGACGA CGACGCCATC 
AGCGAGCTGA AGATGGGCCG CTACTGGAGC AAAGAGGAGA GGAAGCAGCA CGTGGTGCGC 
GCCAAGGAAC AGCGGCAGCG GCGCGAGTTC ATGAAGCAGA GCCGGGCCGA CTGTCTGAAG 
GAGCAGTCCG GCGCCGAGGA CAGAAAGGAG CCCAACATCA TCGAGCTCAG CCACAAAAAG 
ATGATGAAAA AGAGGAACAA GAAAATATTC GACAACTGGA TGACCATCCA AGAACTGCTG 
ACTCATGGCA CCAAGTCTCC AGACGGAACG AGGGTGTACA ACTCGCTCCT GTCCGTGACC 
ACGGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003973780.1
CDS	124..3252
Translation	MGFELDRFKGTVDPDFKCNLCNKVLEDPLTTPCGHVFCSGCVLPWVVQQSSCPVKCQRISAKELNHVLPLKNLILKLEIKCDNHARGCDAVVKLQHLAEHAEMCEYSPVKCRNKGCSEVLNLGDMDAHMRGTCEHRAVGLCEGGCGLMLTYREQRVSGHCCVRALKAHNSALQCKLVCLDREFKKQTIKANKREKALLAQLSAVHGELQMTALKYQKKFTEYSERIDSLTKTVAFSCKEGEAKSVTVVLLREGGSLGFNIVGGRPCSDDIDSISNEGIFVSKIVEKGPADKDGGLQLHDKILEVNGKELSKATHDQAVEAFRTAKEPIVVQVLRRGPAADSQVSDVGTQTDITLQHITALTDLPPSSPPIPELEEYLLPEEHPSGHAYFDPTDFLEGIQQDIEREEMEYEEVDLYRDNIQEKLGLTICYRTDDEDEAGIYISEIDPNSIAAKDGRIREGDKIIQINGVEIQNREDAVALLTREGNQNISLLVARPEIQLDEGWMDDDRNDFLDDLHMDMLEQQHHQAMQFTASMLQQKKHEEDGGTTDTATLLSNHHEKDSGVGRTDESTRNDESSEQENLGDDQTTASNTLGSCRKLNYSQDTLGSADLPFSGESFISADYADADFLGITADECQRFRELLELKCQMRNGGAQGLHCQGAEGQDQEGVDKELELLNEELRTIELECLNIVRAHKMQLLKEQCRESWMLHDSGFRNYNTSIDARRHELSDITELPEKSDKDSSSAYNTGESCRSTPLTLELSPDNSLRRGTENAAPTGAPAAGSSGGRALEPLLFPGQDQASGPSRSKGSSKDPAGGLQAESKERKPGESSKSGRSFSQPHSPYKHAHIPAHAQHYQSYMQLIQQKSAVEYAQSQMSLVSMCRDPISPTDLEPKMEWKVKVRSDGTRYITKRPIRDKLLRERALRIHEERSGMTTDDDAISELKMGRYWSKEERKQHVVRAKEQRQRREFMKQSRADCLKEQSGAEDRKEPNIIELSHKKMMKKRNKKIFDNWMTIQELLTHGTKSPDGTRVYNSLLSVTTV

Target ORF information:

RefSeq Version	XM_003973731.2
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes PDZ domain containing ring finger 3 (pdzrn3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003973731.2

ORF Insert Sequence:

ATGGGATTCG AGCTGGACCG CTTCAAGGGG ACCGTGGACC CGGATTTTAA ATGCAACTTG 
TGCAATAAAG TGTTGGAAGA CCCGCTGACT ACTCCGTGCG GTCACGTCTT CTGCTCCGGC 
TGCGTGCTGC CCTGGGTCGT CCAGCAGAGC AGCTGCCCCG TCAAATGTCA GCGGATCTCG 
GCCAAGGAGC TGAACCACGT CCTGCCGCTG AAGAACCTCA TCCTGAAGCT GGAGATCAAA 
TGTGACAATC ACGCGAGGGG CTGTGATGCG GTGGTGAAGC TGCAGCATCT GGCCGAGCAC 
GCCGAGATGT GCGAGTACTC CCCGGTGAAG TGTCGGAACA AGGGATGCAG CGAGGTGCTG 
AACCTGGGGG ACATGGACGC TCACATGCGC GGGACGTGCG AGCACAGGGC GGTGGGGCTG 
TGCGAGGGGG GCTGCGGGCT GATGCTCACC TACAGGGAGC AGAGAGTCAG CGGCCACTGC 
TGCGTCCGAG CCCTGAAGGC GCACAACAGC GCGCTGCAGT GCAAACTCGT CTGCCTGGAC 
AGAGAGTTCA AGAAGCAGAC TATTAAAGCC AACAAGCGGG AGAAGGCGCT GCTGGCTCAA 
CTGTCTGCCG TGCACGGCGA GCTGCAGATG ACAGCTCTGA AATATCAGAA GAAATTCACC 
GAGTACAGCG AGAGGATCGA CTCCCTCACC AAGACGGTGG CTTTCTCCTG CAAGGAAGGT 
GAAGCTAAAA GTGTGACCGT TGTCCTTCTG CGTGAGGGCG GATCTCTGGG GTTTAACATC 
GTGGGAGGAA GGCCATGCTC GGACGACATT GACAGCATTT CAAACGAAGG CATCTTTGTG 
TCCAAGATTG TGGAGAAAGG TCCTGCTGAC AAAGACGGAG GCCTCCAGCT CCACGACAAA 
ATATTGGAGG TCAATGGCAA AGAGCTCTCC AAGGCCACCC ATGACCAGGC GGTGGAGGCT 
TTCCGCACGG CCAAGGAGCC CATCGTGGTC CAGGTTCTGC GCCGAGGCCC GGCTGCTGAC 
TCCCAGGTCT CGGACGTCGG TACCCAGACT GACATCACCC TTCAGCACAT CACGGCTCTC 
ACAGACCTGC CTCCCTCCTC ACCCCCAATA CCGGAGCTGG AGGAGTATCT GCTCCCTGAG 
GAGCATCCTT CAGGACATGC CTACTTTGAC CCTACGGACT TCCTGGAGGG CATCCAGCAG 
GACATCGAGC GTGAGGAGAT GGAATATGAG GAGGTGGATT TGTACAGAGA CAACATACAG 
GAGAAGCTGG GCCTGACCAT CTGCTACAGA ACTGATGATG AAGATGAAGC TGGGATCTAC 
ATTAGTGAGA TTGATCCCAA CAGCATTGCA GCCAAGGACG GCAGAATTAG AGAAGGTGAC 
AAGATCATCC AGATCAACGG TGTAGAGATC CAGAACAGAG AAGATGCTGT GGCCCTGCTG 
ACCAGAGAGG GGAACCAGAA CATCTCCCTG CTGGTGGCCC GGCCAGAGAT CCAGCTGGAT 
GAAGGCTGGA TGGATGATGA CAGGAACGAC TTTCTGGATG ACCTCCACAT GGACATGTTG 
GAGCAGCAGC ACCATCAGGC CATGCAGTTC ACTGCCAGCA TGCTGCAGCA GAAGAAGCAT 
GAAGAGGATG GAGGCACCAC GGACACGGCC ACGCTCCTGT CCAACCACCA CGAGAAGGAC 
AGCGGGGTGG GCCGCACCGA CGAGAGCACG CGGAACGACG AGAGCTCCGA GCAGGAGAAC 
CTCGGCGACG ACCAGACCAC GGCCTCCAAC ACGCTCGGCA GCTGCCGGAA GCTGAACTAC 
AGCCAGGACA CCCTCGGCAG CGCCGACCTG CCCTTCAGCG GCGAGTCGTT CATCTCCGCG 
GACTACGCAG ACGCCGACTT CCTGGGAATC ACGGCTGACG AGTGCCAGCG CTTTCGGGAG 
CTCCTGGAAC TGAAGTGTCA GATGAGGAAC GGCGGAGCTC AGGGCCTCCA CTGCCAAGGC 
GCTGAAGGTC AGGACCAGGA AGGTGTGGAC AAAGAGCTAG AGCTGCTCAA TGAGGAGCTA 
CGGACCATCG AGCTGGAGTG CCTGAACATC GTCCGCGCTC ACAAGATGCA GCTGCTGAAG 
GAGCAGTGCC GGGAATCCTG GATGCTCCAC GACAGCGGCT TCCGCAACTA CAACACCAGC 
ATTGACGCAC GCCGCCACGA GCTCTCAGAC ATCACAGAGC TGCCCGAAAA GTCTGACAAA 
GACAGCTCGA GCGCCTACAA CACCGGGGAG AGCTGCCGCA GCACCCCCCT CACGCTGGAG 
CTGTCTCCAG ACAACTCGCT TCGTAGAGGG ACGGAGAACG CGGCTCCGAC CGGAGCGCCG 
GCCGCCGGCA GCTCCGGTGG GAGGGCGCTC GAGCCTCTTT TATTCCCTGG CCAAGATCAA 
GCATCAGGCC CCAGCCGGAG CAAAGGCTCC TCCAAGGATC CGGCTGGAGG TCTGCAAGCC 
GAAAGCAAGG AAAGGAAGCC GGGCGAGTCC AGCAAGTCCG GGCGGAGCTT TTCCCAGCCG 
CACTCTCCAT ACAAACACGC CCACATCCCC GCCCACGCCC AGCACTACCA GAGCTACATG 
CAGCTGATCC AGCAGAAATC GGCTGTGGAG TACGCCCAGA GCCAGATGAG CCTGGTTAGC 
ATGTGCCGCG ACCCCATCAG CCCCACCGAC CTCGAGCCTA AGATGGAGTG GAAGGTGAAG 
GTGCGCAGCG ACGGCACCCG CTACATCACC AAGCGGCCCA TCCGGGACAA GCTGCTGCGG 
GAGCGCGCCC TGCGCATTCA CGAGGAGCGC AGCGGCATGA CCACGGACGA CGACGCCATC 
AGCGAGCTGA AGATGGGCCG CTACTGGAGC AAAGAGGAGA GGAAGCAGCA CGTGGTGCGC 
GCCAAGGAAC AGCGGCAGCG GCGCGAGTTC ATGAAGCAGA GCCGGGCCGA CTGTCTGAAG 
GAGCAGTCCG GCGCCGAGGA CAGAAAGGAG CCCAACATCA TCGAGCTCAG CCACAAAAAG 
ATGATGAAAA AGAGGAACAA GAAAATATTC GACAACTGGA TGACCATCCA AGAACTGCTG 
ACTCATGGCA CCAAGTCTCC AGACGGAACG AGGGTGTACA ACTCGCTCCT GTCCGTGACC 
ACGGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pdzrn3 ( XM_003973731.3 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_003973780.2 Takifugu rubripes PDZ domain containing ring finger 3 (pdzrn3), mRNA.

Price & Availability↑

CloneID	OTo55546
Clone ID Related Accession (Same CDS sequence)	XM_003973731.3
Accession Version	XM_003973731.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3132bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562169600000
Organism	Takifugu rubripes(torafugu)
Product	LOW QUALITY PROTEIN: E3 ubiquitin-protein ligase PDZRN3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042303.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_003973731.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGGGATTCG AGCTGGACCG CTTCAAGGGG ACCGTGGACC CGGATTTTAA ATGCAACTTG 
TGCAATAAAG TGTTGGAAGA CCCGCTGACT ACTCCGTGCG GTCACGTCTT CTGCTCCGGC 
TGCGTGCTGC CCTGGGTCGT CCAGCAGAGC AGCTGCCCCG TCAAATGTCA GCGGATCTCG 
GCCAAGGAGC TGAACCACGT CCTGCCGCTG AAGAACCTCA TCCTGAAGCT GGAGATCAAA 
TGTGACAATC ACGCGAGGGG CTGTGATGCG GTGGTGAAGC TGCAGCATCT GGCCGAGCAC 
GCCGAGATGT GCGAGTACTC CCCGGTGAAG TGTCGGAACA AGGGATGCAG CGAGGTGCTG 
AACCTGGGGG ACATGGACGC TCATATGCGC GGGACGTGCG AGCACAGGGC GGTGGGGCTG 
TGCGAGGGGG GCTGCGGGCT GATGCTCACC TACAGGGAGC AGAGAGTCAG CGGCCACTGC 
TGCGTCCGAG CCCTGAAGGC GCACAACAGC GCGCTGCAGT GCAAACTCGT CTGCCTGGAC 
AGAGAGTTCA AGAAGCAGAC TATTAAAGCC AACAAGCGGG AGAAGGCGCT GCTGGCTCAA 
CTGTCTGCCG TGCACGGCGA GCTGCAGATG ACAGCTCTGA AATATCAGAA GAAATTCACC 
GAGTACAGCG AGAGGATCGA CTCCCTCACC AAGACGGTGG CTTTCTCCTG CAAGGAAGGT 
GAAGCTAAAA GTGTGACCGT TGTCCTTCTG CGTGAGGGCG GATCTCTGGG GTTTAACATC 
GTGGGAGGAA GGCCATGCTC GGACGACATT GACAGCATTT CAAACGAAGG CATCTTTGTG 
TCCAAGATTG TGGAGAAAGG TCCAGCTGAC AAAGACGGAG GCCTCCAGCT CCACGACAAA 
ATATTGGAGG TCAATGGCAA AGAGCTCTCC AAGGCCACCC ATGACCAGGC GGTGGAGGCT 
TTCCGCACGG CCAAGGAGCC CATCGTGGTC CAGGTTCTGC GCCGAGGCCC GGCTGCTGAC 
TCCCAGGTCT CGGACGTCGG TACCCAGACT GACATCACCC TTCAGCACAT CACGGCTCTC 
ACAGACCTGC CTCCCTCCTC ACCCCCAATA CCGGAGCTGG AGGAGTATCT GCTCCCTGAG 
GAGCATCCTT CAGGACATGC CTACTTTGAC CCTACGGACT TCCTGGAGGG CATCCAGCAG 
GACATCGAGC GTGAGGAGAT GGAATATGAG GAGGTGGATT TGTACAGAGA CAACATACAG 
GAGAAGCTGG GCCTGACCAT CTGCTACAGA ACTGATGATG AAGATGAAGC TGGGATCTAC 
ATTAGTGAGA TTGATCCCAA CAGCATTGCA GCCAAGGACG GCAGAATTAG AGAAGGTGAC 
AAGATCATCC AGATCAACGG TGTAGAGATC CAGAACAGAG AAGATGCTGT GGCCCTGCTG 
ACCAGAGAGG GGAACCAGAA CATCTCCCTG CTGGTGGCCC GGCCAGAGAT CCAGCTGGAT 
GAAGGCTGGA TGGATGATGA CAGGAACGAC TTTCTGGATG ACCTCCACAT GGACATGTTG 
GAGCAGCAGC ACCATCAGGC CATGCAGTTC ACTGCCAGCA TGCTGCAGCA GAAGAAGCAT 
GAAGAGGATG GAGGCACCAC GGACACGGCC ACGCTCCTGT CCAACCACCA CGAGAAGGAC 
AGCGGGGTGG GCCGCACCGA CGAGAGCACG CGGAACGACG AGAGCTCCGA GCAGGAGAAC 
CTCGGCGACG ACCAGACCAC GGCCTCCAAC ACGCTCGGCA GCTGCCGGAA GCTGAACTAC 
AGCCAGGACA CCCTCGGCAG CGCCGACCTG CCCTTCAGCG GCGAGTCGTT CATCTCCGCG 
GACTACGCAG ACGCCGACTT CCTGGGAATC ACGGCTGACG AGTGCCAGCG CTTTCGGGAG 
CTCCTGGAAC TGAAGTGTCA GATGAGGAAC GGCGGAGCTC AGGGCCTCCA CTGCCAAGGC 
GCTGAAGGTC AGGACCAGGA AGGTGTGGAC AAAGAGCTAG AGCTGCTCAA TGAGGAGCTA 
CGGACCATCG AGCTGGAGTG CCTGAACATC GTCCGCGCTC ACAAGATGCA GCTGCTGAAG 
GAGCAGTGCC GGGAATCCTG GATGCTCCAC GACAGCGGCT TCCGCAACTA CAACACCAGC 
ATTGACGCAC GCCGCCACGA GCTCTCAGAC ATCACAGAGC TGCCCGAGAA GTCTGACAAA 
GACAGCTCGA GCGCCTACAA CACCGGGGAG AGCTGCCGCA GCACCCCCCT CACGCTGGAG 
CTGTCTCCAG ACAACTCGCT TCGTAGAGGG ACGGAGAACG CGGCTNNCCC GACCGGAGCG 
CCGGCCGCCG GCAGCTCCGG TGGGAGGGCG CTCGAGCCTC TTTTATTCCC TGGCCAAGAT 
CAAGCATCAG GCCCCAGCCG GAGCAAAGGC TCCTCCAAGG ATCCGGCTGG AGGTCTGCAA 
GCCGAAAGCA AGGAAAGGAA GCCGGGCGAG TCCAGCAAGT CCGGGCGGAG CTTTTCCCAG 
CCGCACTCTC CATACAAACA CGCCCACATC CCCGCCCACG CCCAGCACTA CCAGAGCTAC 
ATGCAGCTGA TCCAGCAGAA ATCGGCTGTG GAGTACGCCC AGAGCCAGAT GAGCCTGGTT 
AGCATGTGCC GCGACCCCAT CGGCCCCACC GACCTCGAGC CTAAGATGGA GTGGAAGGTG 
AAGGTGCGCA GCGACGGCAC CCGCTACATC ACCAAGCGGC CCATCCGGGA CAAGCTGCTG 
CGGGAGCGTG CCCTGCGCAT TCACGAGGAG CGCAGCGGCA TGACCACGGA CGACGACGCC 
ATCAGCGAGC TGAAGATGGG CCGCTACTGG AGCAAAGAGG AGAGGAAGCA GCACGTGGTG 
CGCGCCAAGG AACAGCGGCA GCGGCGCGAG TTCATGAAGC AGAGCCGGGC CGACTGTCTG 
AAGGAGCAGT CCGGCGCCGA GGACAGAAAG GAGCCCAACA TCATCGAGCT CAGCCACAAA 
AAGATGATGA AAAAGAGGAA CAAGAAAATA TTCGACAACT GGATGACCAT CCAAGAACTG 
CTGACTCATG GCACCAAGTC TCCAGACGGA ACGAGGGTGT ACAACTCGCT CCTGTCCGTG 
ACCACGGTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003973780.2
CDS	136..3267
Translation	MGFELDRFKGTVDPDFKCNLCNKVLEDPLTTPCGHVFCSGCVLPWVVQQSSCPVKCQRISAKELNHVLPLKNLILKLEIKCDNHARGCDAVVKLQHLAEHAEMCEYSPVKCRNKGCSEVLNLGDMDAHMRGTCEHRAVGLCEGGCGLMLTYREQRVSGHCCVRALKAHNSALQCKLVCLDREFKKQTIKANKREKALLAQLSAVHGELQMTALKYQKKFTEYSERIDSLTKTVAFSCKEGEAKSVTVVLLREGGSLGFNIVGGRPCSDDIDSISNEGIFVSKIVEKGPADKDGGLQLHDKILEVNGKELSKATHDQAVEAFRTAKEPIVVQVLRRGPAADSQVSDVGTQTDITLQHITALTDLPPSSPPIPELEEYLLPEEHPSGHAYFDPTDFLEGIQQDIEREEMEYEEVDLYRDNIQEKLGLTICYRTDDEDEAGIYISEIDPNSIAAKDGRIREGDKIIQINGVEIQNREDAVALLTREGNQNISLLVARPEIQLDEGWMDDDRNDFLDDLHMDMLEQQHHQAMQFTASMLQQKKHEEDGGTTDTATLLSNHHEKDSGVGRTDESTRNDESSEQENLGDDQTTASNTLGSCRKLNYSQDTLGSADLPFSGESFISADYADADFLGITADECQRFRELLELKCQMRNGGAQGLHCQGAEGQDQEGVDKELELLNEELRTIELECLNIVRAHKMQLLKEQCRESWMLHDSGFRNYNTSIDARRHELSDITELPEKSDKDSSSAYNTGESCRSTPLTLELSPDNSLRRGTENAAnullPTGAPAAGSSGGRALEPLLFPGQDQASGPSRSKGSSKDPAGGLQAESKERKPGESSKSGRSFSQPHSPYKHAHIPAHAQHYQSYMQLIQQKSAVEYAQSQMSLVSMCRDPIGPTDLEPKMEWKVKVRSDGTRYITKRPIRDKLLRERALRIHEERSGMTTDDDAISELKMGRYWSKEERKQHVVRAKEQRQRREFMKQSRADCLKEQSGAEDRKEPNIIELSHKKMMKKRNKKIFDNWMTIQELLTHGTKSPDGTRVYNSLLSVTTV

Target ORF information:

RefSeq Version	XM_003973731.3
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes PDZ domain containing ring finger 3 (pdzrn3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003973731.3

ORF Insert Sequence:

ATGGGATTCG AGCTGGACCG CTTCAAGGGG ACCGTGGACC CGGATTTTAA ATGCAACTTG 
TGCAATAAAG TGTTGGAAGA CCCGCTGACT ACTCCGTGCG GTCACGTCTT CTGCTCCGGC 
TGCGTGCTGC CCTGGGTCGT CCAGCAGAGC AGCTGCCCCG TCAAATGTCA GCGGATCTCG 
GCCAAGGAGC TGAACCACGT CCTGCCGCTG AAGAACCTCA TCCTGAAGCT GGAGATCAAA 
TGTGACAATC ACGCGAGGGG CTGTGATGCG GTGGTGAAGC TGCAGCATCT GGCCGAGCAC 
GCCGAGATGT GCGAGTACTC CCCGGTGAAG TGTCGGAACA AGGGATGCAG CGAGGTGCTG 
AACCTGGGGG ACATGGACGC TCATATGCGC GGGACGTGCG AGCACAGGGC GGTGGGGCTG 
TGCGAGGGGG GCTGCGGGCT GATGCTCACC TACAGGGAGC AGAGAGTCAG CGGCCACTGC 
TGCGTCCGAG CCCTGAAGGC GCACAACAGC GCGCTGCAGT GCAAACTCGT CTGCCTGGAC 
AGAGAGTTCA AGAAGCAGAC TATTAAAGCC AACAAGCGGG AGAAGGCGCT GCTGGCTCAA 
CTGTCTGCCG TGCACGGCGA GCTGCAGATG ACAGCTCTGA AATATCAGAA GAAATTCACC 
GAGTACAGCG AGAGGATCGA CTCCCTCACC AAGACGGTGG CTTTCTCCTG CAAGGAAGGT 
GAAGCTAAAA GTGTGACCGT TGTCCTTCTG CGTGAGGGCG GATCTCTGGG GTTTAACATC 
GTGGGAGGAA GGCCATGCTC GGACGACATT GACAGCATTT CAAACGAAGG CATCTTTGTG 
TCCAAGATTG TGGAGAAAGG TCCAGCTGAC AAAGACGGAG GCCTCCAGCT CCACGACAAA 
ATATTGGAGG TCAATGGCAA AGAGCTCTCC AAGGCCACCC ATGACCAGGC GGTGGAGGCT 
TTCCGCACGG CCAAGGAGCC CATCGTGGTC CAGGTTCTGC GCCGAGGCCC GGCTGCTGAC 
TCCCAGGTCT CGGACGTCGG TACCCAGACT GACATCACCC TTCAGCACAT CACGGCTCTC 
ACAGACCTGC CTCCCTCCTC ACCCCCAATA CCGGAGCTGG AGGAGTATCT GCTCCCTGAG 
GAGCATCCTT CAGGACATGC CTACTTTGAC CCTACGGACT TCCTGGAGGG CATCCAGCAG 
GACATCGAGC GTGAGGAGAT GGAATATGAG GAGGTGGATT TGTACAGAGA CAACATACAG 
GAGAAGCTGG GCCTGACCAT CTGCTACAGA ACTGATGATG AAGATGAAGC TGGGATCTAC 
ATTAGTGAGA TTGATCCCAA CAGCATTGCA GCCAAGGACG GCAGAATTAG AGAAGGTGAC 
AAGATCATCC AGATCAACGG TGTAGAGATC CAGAACAGAG AAGATGCTGT GGCCCTGCTG 
ACCAGAGAGG GGAACCAGAA CATCTCCCTG CTGGTGGCCC GGCCAGAGAT CCAGCTGGAT 
GAAGGCTGGA TGGATGATGA CAGGAACGAC TTTCTGGATG ACCTCCACAT GGACATGTTG 
GAGCAGCAGC ACCATCAGGC CATGCAGTTC ACTGCCAGCA TGCTGCAGCA GAAGAAGCAT 
GAAGAGGATG GAGGCACCAC GGACACGGCC ACGCTCCTGT CCAACCACCA CGAGAAGGAC 
AGCGGGGTGG GCCGCACCGA CGAGAGCACG CGGAACGACG AGAGCTCCGA GCAGGAGAAC 
CTCGGCGACG ACCAGACCAC GGCCTCCAAC ACGCTCGGCA GCTGCCGGAA GCTGAACTAC 
AGCCAGGACA CCCTCGGCAG CGCCGACCTG CCCTTCAGCG GCGAGTCGTT CATCTCCGCG 
GACTACGCAG ACGCCGACTT CCTGGGAATC ACGGCTGACG AGTGCCAGCG CTTTCGGGAG 
CTCCTGGAAC TGAAGTGTCA GATGAGGAAC GGCGGAGCTC AGGGCCTCCA CTGCCAAGGC 
GCTGAAGGTC AGGACCAGGA AGGTGTGGAC AAAGAGCTAG AGCTGCTCAA TGAGGAGCTA 
CGGACCATCG AGCTGGAGTG CCTGAACATC GTCCGCGCTC ACAAGATGCA GCTGCTGAAG 
GAGCAGTGCC GGGAATCCTG GATGCTCCAC GACAGCGGCT TCCGCAACTA CAACACCAGC 
ATTGACGCAC GCCGCCACGA GCTCTCAGAC ATCACAGAGC TGCCCGAGAA GTCTGACAAA 
GACAGCTCGA GCGCCTACAA CACCGGGGAG AGCTGCCGCA GCACCCCCCT CACGCTGGAG 
CTGTCTCCAG ACAACTCGCT TCGTAGAGGG ACGGAGAACG CGGCTNNCCC GACCGGAGCG 
CCGGCCGCCG GCAGCTCCGG TGGGAGGGCG CTCGAGCCTC TTTTATTCCC TGGCCAAGAT 
CAAGCATCAG GCCCCAGCCG GAGCAAAGGC TCCTCCAAGG ATCCGGCTGG AGGTCTGCAA 
GCCGAAAGCA AGGAAAGGAA GCCGGGCGAG TCCAGCAAGT CCGGGCGGAG CTTTTCCCAG 
CCGCACTCTC CATACAAACA CGCCCACATC CCCGCCCACG CCCAGCACTA CCAGAGCTAC 
ATGCAGCTGA TCCAGCAGAA ATCGGCTGTG GAGTACGCCC AGAGCCAGAT GAGCCTGGTT 
AGCATGTGCC GCGACCCCAT CGGCCCCACC GACCTCGAGC CTAAGATGGA GTGGAAGGTG 
AAGGTGCGCA GCGACGGCAC CCGCTACATC ACCAAGCGGC CCATCCGGGA CAAGCTGCTG 
CGGGAGCGTG CCCTGCGCAT TCACGAGGAG CGCAGCGGCA TGACCACGGA CGACGACGCC 
ATCAGCGAGC TGAAGATGGG CCGCTACTGG AGCAAAGAGG AGAGGAAGCA GCACGTGGTG 
CGCGCCAAGG AACAGCGGCA GCGGCGCGAG TTCATGAAGC AGAGCCGGGC CGACTGTCTG 
AAGGAGCAGT CCGGCGCCGA GGACAGAAAG GAGCCCAACA TCATCGAGCT CAGCCACAAA 
AAGATGATGA AAAAGAGGAA CAAGAAAATA TTCGACAACT GGATGACCAT CCAAGAACTG 
CTGACTCATG GCACCAAGTC TCCAGACGGA ACGAGGGTGT ACAACTCGCT CCTGTCCGTG 
ACCACGGTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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pdzrn3 cDNA ORF clone, Takifugu rubripes(torafugu)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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