LOC588813 cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC588813
Entrez Gene ID	588813
Full Name	uncharacterized LOC588813
Gene Type	protein-coding
Organism	Strongylocentrotus purpuratus(purple sea urchin)

mRNA and Protein(s)

mRNA	Protein	Name
XM_788477.3	XP_793570.2	uncharacterized protein LOC588813
XM_788477.4	XP_793570.3	probable ATP-dependent RNA helicase DDX20

Pathways from BioSystems
Gene Ontology

The following LOC588813 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC588813 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSd04521	XM_788477.3 Latest version!	Strongylocentrotus purpuratus uncharacterized LOC588813 (LOC588813), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OSd41884	XM_788477.4 Latest version!	Strongylocentrotus purpuratus probable ATP-dependent RNA helicase DDX20 (LOC588813), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC588813 ( XM_788477.3 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_793570.2 Strongylocentrotus purpuratus uncharacterized LOC588813 (LOC588813), mRNA.

Price & Availability↑

CloneID	OSd04521
Clone ID Related Accession (Same CDS sequence)	XM_788477.3
Accession Version	XM_788477.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3573bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1427040000000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	uncharacterized protein LOC588813
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011975137.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 24, 2015 this sequence version replaced XM_788477.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Strongylocentrotus purpuratus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACGC AGACCGGCCA AGCAAGGATG GGAGCTCCGG GTTCCTTGAG AACAGCCCAT 
GCTATCAATG AAAGGGAGAG AACCAGTGAT GTTTTGACTG AAGAGAACAG CAATGTTGAC 
TTCTCATCTA TGTTCCTTTC CGAACCCGTG ACGGCCGGAT TACAGAAAGT TGGTTTTGAG 
AAACCTTCAC CTATTCAATT AAAGGCAATA CCACTTGGGA GATGCGGTTT AGACTTGATT 
GTCCAGGCTA AGTCTGGCAC AGGGAAGACG TGTGTGTTCA GTGTCATAGC TCTAGAGGGA 
ATAGATCTCA CCAATCCATC TACTCAGGTT CTGATTCTAG CCCCCACGAG AGAGATTGCA 
GTCCAGATTC AAGACACTAT TCGAGCTATA GGATGTGAGA TGGAGGGACT ACGTTCTCAT 
GTGTTTATCG GAGGTACTCT ATTTGGACCA GACAGGCAGA AGTTGAAGAA GTGTCACATA 
GCTGTTGGTA CACCAGGACG TATCAAACAG CTAATTGAGT ATGAAGTATT GAAGACCGGA 
ACTATCAGGC TTTTTGTCTT GGATGAAGCA GACAAACTAC TTGATGATAC ATTTCAGGAG 
CAAGTCAATT GGATTTACAA CCACCTTTCA GATAACAAGC AGATGTTAGC ACTCTCTGCA 
ACCTACCCCG AGTACCTTGC CAAACATCTC ACTAAATATA TGAGAGAACC TATGTTTGTT 
CGACTCAATC CAAAGGATCT CGCATTAAGA GGCATCAAGC AGTTGTATGT GGAGTTACCT 
GGCCACTCTC TTCCTAACAA AGCATTTGAG ATCAAGGTTA TCAAACTGCT GAAAATCCTG 
ACTCAAGTAT CCTTCAATCA ATGTCTCATC TTCTCCAACT TACAGACAAG GGCTCAGAAT 
CTATGTGACA TCCTCTGTGA CTCTGGTTGG CCTAGTGAGT GTATATCAGG TGCACAGGAG 
CAGTCTCAAC GACTCAGTGC TATGGCCAAG CTAAAGAAAT TCCAGTGTCG GATCCTCATC 
TCTACAGATC TAACTGCCAG AGGTATCGAT GCTGAGAATG TCAACCTGAT CATCAACCTA 
GACATTCCAA CCGACACCAA GACCTACCTT CATCGCATTG GGAGGGCGGG GCGCTTCGGT 
ACCCATGGAG CTGCTATCAG CCTTGCTTCC AAGGGTAGGG AGATGGAACT ACTCAGGAAT 
ATCCAGCGTC AGTGTCGAGT GACAATGACA GAGCTACCTG ATCCTCCTCC ATCTGATTTG 
CTGAAGCAGG ACCACAACTC AACGGGACTT CAACGGGTTT CTATTACACC ACATTCTCTC 
CTCCCTGCAG CCAAGATGGA TTCTACAAGA AATACCACCA CAGATGATCT GGAAAACAAT 
GCTACTGCTA AAACATCAGA TGATGCTTTG TTGACTTCCT CAGTTGTGAA TAAAGAAGAA 
GACCCCATGC CAAAATCTGT TATCGCAAAT GGGACGGATG AACAACATTC ACTCCATATG 
GGAATCAATG GGAGTTGTCA CAAGATGGAA GATAGTGCTG ATAGTTTAGC GGATAGGAAG 
ACAACAAAGA TTGTGGAAAG TATGGTGAAC CAAAATCCTA TACCAAAGAC TAGAAGAGGA 
GTCCATGAAC CACAGAAAGT TGATGCATGT ATCCAAACTA TGCAGGAAGA TGTTGTTCAA 
CCAAAGGCCA AGACATCACA GATAAGATAC GTTACTGGAA GTGGGGATCA CAAAGACAGA 
CCGACATTAC CGATTGGCTT TGAAGATGCC CCAGAAGTGA GGAAGCTCAG TCTTCCTCAA 
GTGTCCCATA TTAAAAGAGG AGATGGATCA AGAAGAGCTC GTACCTGGTA TGAAGGTAAA 
GAATCGTTTG AAAAGTTCAT GAAGGGTCAA ACAGTGGAGA CTAAAGGTCA AGGGTCAGAA 
GATGACAGGG TAGACAAGGA GACGGATGTC ATTGAGCAGA CAGTGAAAAA TGACAAGGAA 
CTGTCGGTTG AAATATCACA GTTAGAAGGT GAAGAAAATG ATGCAGATGT GTCTTCTAGA 
AACTCTCATG TCACATCAAG CAAAGATCTC AATATTGGTG ATGACTATCA GAACAAATAC 
AAAGATGAAG TCTACAATGG GAGTGGAAAT GCAGATCGTG AAATTGCGCA GAAAAGGATC 
TCTGGCTTAA CACCCATCCA AGAGATAGAG AGGTACAGGG GTCTACTCTG TGATACATTT 
ATACGCAATC ACTTAGGAAG GGGAGACAAC TCTACTTTGA TCAAACTTGA ACATCATGAT 
GAGGCAATTA CAGCTGTAAC AGAGGCAAAA GAGACAGAGT CAGTAGAAAG TGTGACTCCT 
TTAATGGACA TTTCTTCAGT CGTGAACAAG CAAGTGGCAA GAAAGTTAGG AGATAAGCAA 
GATGCAAAAC AGACCCAGTG CACATCAAAA GTAACCAAAG ACATTCAAGA AAGCGATGAG 
ACAGTCAAAG TTCCACAGGT TGAAAGTAGG CTTCATCACC AAGTCTCCAA TGGTTCCCCA 
GACATCCGAT CAAGGAATGA ACCTGAAACA GAAACTAAAC CAAAGGTTAG GAATACTACA 
GGAGGAAAGA AGAAAGGGAA GCAGCAAAGA ATCAAACAAC ATGTCTTTCA GATGAGTACC 
CTCGGTGCTC TGATGTCTCC CAAAGAACTT GATGTAGCTC TCATCAGCAA TCCAAATACC 
AGCTCGTGTG ATGGTGATTT GGACACTAAT GATACTGACA GAGAAAAAGT TGAAAGATTT 
CCTTTGTGTA ACAAGATAAA TGTGACACAT CAAGTTCATC ATGCAAAAAA TGGTTATGAA 
GAAAGCCAAA TTCCAGAATC GGGTGAAAGA GAAACCTTGA AGGACAAACA AGTGAGAACA 
TCAGTTCAGA AAAGTCTTTC CCTAGAAAAT GACAGTTCTG TGTCATCATT AGATGACCCT 
GTGTGGGCAA TTTTCAAAAA TTTCATTCCA GCATGTCTTC CATCAGAAGT TGGTAGAAAG 
GACGCCACAC TGTCAGATCA GTCTTCAAGC AGTTTGTGTG AGAGTAACAC AAGCACTTGT 
AGCTCTTCTT CAAGTGAAAC TGATAGTGGA AGCAGCGGTA CCAATGGAGA AGAAGAGGAA 
GACGGCAAGG AGGCATCTGA GAGCAACCGC AAGAATAAGT GGAAAGGAAA GGAACCTGCA 
AGAAATGGGA GTGGAAAGCA CCACATGGGG TTAAATCAAG CCAAAGCAAA GGAGAAGAAT 
CTTTCTAAAG CTAATAGCAG CTACAATGAA GAAAAGCACT ACTGTGAAGG ACAAAATAAA 
TATCCACAAT GTCCAGATGA TCCTAGTTGG ACAACTGAAC AATTTCCTAA TCAACCAAGC 
TGTAATGTAG AGGGTTGGGA GTATGATGGA AGCTATGATT ACTATGCTGC TGCTCCAATA 
CCTGGAGCTG CATACCATGG AAGGCCATAC TCAAGTTACT CGCCTCAGGG ATACACAAAT 
AGTTATTGGA ATGACTATTA CCATGGATAT GCCCAACAAC CTTATACTGG GTACCCCTAT 
TTCTCTTCTT ATGATCCTCC CCATTATTCT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_793570.2
CDS	184..3756
Translation	METQTGQARMGAPGSLRTAHAINERERTSDVLTEENSNVDFSSMFLSEPVTAGLQKVGFEKPSPIQLKAIPLGRCGLDLIVQAKSGTGKTCVFSVIALEGIDLTNPSTQVLILAPTREIAVQIQDTIRAIGCEMEGLRSHVFIGGTLFGPDRQKLKKCHIAVGTPGRIKQLIEYEVLKTGTIRLFVLDEADKLLDDTFQEQVNWIYNHLSDNKQMLALSATYPEYLAKHLTKYMREPMFVRLNPKDLALRGIKQLYVELPGHSLPNKAFEIKVIKLLKILTQVSFNQCLIFSNLQTRAQNLCDILCDSGWPSECISGAQEQSQRLSAMAKLKKFQCRILISTDLTARGIDAENVNLIINLDIPTDTKTYLHRIGRAGRFGTHGAAISLASKGREMELLRNIQRQCRVTMTELPDPPPSDLLKQDHNSTGLQRVSITPHSLLPAAKMDSTRNTTTDDLENNATAKTSDDALLTSSVVNKEEDPMPKSVIANGTDEQHSLHMGINGSCHKMEDSADSLADRKTTKIVESMVNQNPIPKTRRGVHEPQKVDACIQTMQEDVVQPKAKTSQIRYVTGSGDHKDRPTLPIGFEDAPEVRKLSLPQVSHIKRGDGSRRARTWYEGKESFEKFMKGQTVETKGQGSEDDRVDKETDVIEQTVKNDKELSVEISQLEGEENDADVSSRNSHVTSSKDLNIGDDYQNKYKDEVYNGSGNADREIAQKRISGLTPIQEIERYRGLLCDTFIRNHLGRGDNSTLIKLEHHDEAITAVTEAKETESVESVTPLMDISSVVNKQVARKLGDKQDAKQTQCTSKVTKDIQESDETVKVPQVESRLHHQVSNGSPDIRSRNEPETETKPKVRNTTGGKKKGKQQRIKQHVFQMSTLGALMSPKELDVALISNPNTSSCDGDLDTNDTDREKVERFPLCNKINVTHQVHHAKNGYEESQIPESGERETLKDKQVRTSVQKSLSLENDSSVSSLDDPVWAIFKNFIPACLPSEVGRKDATLSDQSSSSLCESNTSTCSSSSSETDSGSSGTNGEEEEDGKEASESNRKNKWKGKEPARNGSGKHHMGLNQAKAKEKNLSKANSSYNEEKHYCEGQNKYPQCPDDPSWTTEQFPNQPSCNVEGWEYDGSYDYYAAAPIPGAAYHGRPYSSYSPQGYTNSYWNDYYHGYAQQPYTGYPYFSSYDPPHYS

Target ORF information:

RefSeq Version	XM_788477.3
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus uncharacterized LOC588813 (LOC588813), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_788477.3

ORF Insert Sequence:

ATGGAGACGC AGACCGGCCA AGCAAGGATG GGAGCTCCGG GTTCCTTGAG AACAGCCCAT 
GCTATCAATG AAAGGGAGAG AACCAGTGAT GTTTTGACTG AAGAGAACAG CAATGTTGAC 
TTCTCATCTA TGTTCCTTTC CGAACCCGTG ACGGCCGGAT TACAGAAAGT TGGTTTTGAG 
AAACCTTCAC CTATTCAATT AAAGGCAATA CCACTTGGGA GATGCGGTTT AGACTTGATT 
GTCCAGGCTA AGTCTGGCAC AGGGAAGACG TGTGTGTTCA GTGTCATAGC TCTAGAGGGA 
ATAGATCTCA CCAATCCATC TACTCAGGTT CTGATTCTAG CCCCCACGAG AGAGATTGCA 
GTCCAGATTC AAGACACTAT TCGAGCTATA GGATGTGAGA TGGAGGGACT ACGTTCTCAT 
GTGTTTATCG GAGGTACTCT ATTTGGACCA GACAGGCAGA AGTTGAAGAA GTGTCACATA 
GCTGTTGGTA CACCAGGACG TATCAAACAG CTAATTGAGT ATGAAGTATT GAAGACCGGA 
ACTATCAGGC TTTTTGTCTT GGATGAAGCA GACAAACTAC TTGATGATAC ATTTCAGGAG 
CAAGTCAATT GGATTTACAA CCACCTTTCA GATAACAAGC AGATGTTAGC ACTCTCTGCA 
ACCTACCCCG AGTACCTTGC CAAACATCTC ACTAAATATA TGAGAGAACC TATGTTTGTT 
CGACTCAATC CAAAGGATCT CGCATTAAGA GGCATCAAGC AGTTGTATGT GGAGTTACCT 
GGCCACTCTC TTCCTAACAA AGCATTTGAG ATCAAGGTTA TCAAACTGCT GAAAATCCTG 
ACTCAAGTAT CCTTCAATCA ATGTCTCATC TTCTCCAACT TACAGACAAG GGCTCAGAAT 
CTATGTGACA TCCTCTGTGA CTCTGGTTGG CCTAGTGAGT GTATATCAGG TGCACAGGAG 
CAGTCTCAAC GACTCAGTGC TATGGCCAAG CTAAAGAAAT TCCAGTGTCG GATCCTCATC 
TCTACAGATC TAACTGCCAG AGGTATCGAT GCTGAGAATG TCAACCTGAT CATCAACCTA 
GACATTCCAA CCGACACCAA GACCTACCTT CATCGCATTG GGAGGGCGGG GCGCTTCGGT 
ACCCATGGAG CTGCTATCAG CCTTGCTTCC AAGGGTAGGG AGATGGAACT ACTCAGGAAT 
ATCCAGCGTC AGTGTCGAGT GACAATGACA GAGCTACCTG ATCCTCCTCC ATCTGATTTG 
CTGAAGCAGG ACCACAACTC AACGGGACTT CAACGGGTTT CTATTACACC ACATTCTCTC 
CTCCCTGCAG CCAAGATGGA TTCTACAAGA AATACCACCA CAGATGATCT GGAAAACAAT 
GCTACTGCTA AAACATCAGA TGATGCTTTG TTGACTTCCT CAGTTGTGAA TAAAGAAGAA 
GACCCCATGC CAAAATCTGT TATCGCAAAT GGGACGGATG AACAACATTC ACTCCATATG 
GGAATCAATG GGAGTTGTCA CAAGATGGAA GATAGTGCTG ATAGTTTAGC GGATAGGAAG 
ACAACAAAGA TTGTGGAAAG TATGGTGAAC CAAAATCCTA TACCAAAGAC TAGAAGAGGA 
GTCCATGAAC CACAGAAAGT TGATGCATGT ATCCAAACTA TGCAGGAAGA TGTTGTTCAA 
CCAAAGGCCA AGACATCACA GATAAGATAC GTTACTGGAA GTGGGGATCA CAAAGACAGA 
CCGACATTAC CGATTGGCTT TGAAGATGCC CCAGAAGTGA GGAAGCTCAG TCTTCCTCAA 
GTGTCCCATA TTAAAAGAGG AGATGGATCA AGAAGAGCTC GTACCTGGTA TGAAGGTAAA 
GAATCGTTTG AAAAGTTCAT GAAGGGTCAA ACAGTGGAGA CTAAAGGTCA AGGGTCAGAA 
GATGACAGGG TAGACAAGGA GACGGATGTC ATTGAGCAGA CAGTGAAAAA TGACAAGGAA 
CTGTCGGTTG AAATATCACA GTTAGAAGGT GAAGAAAATG ATGCAGATGT GTCTTCTAGA 
AACTCTCATG TCACATCAAG CAAAGATCTC AATATTGGTG ATGACTATCA GAACAAATAC 
AAAGATGAAG TCTACAATGG GAGTGGAAAT GCAGATCGTG AAATTGCGCA GAAAAGGATC 
TCTGGCTTAA CACCCATCCA AGAGATAGAG AGGTACAGGG GTCTACTCTG TGATACATTT 
ATACGCAATC ACTTAGGAAG GGGAGACAAC TCTACTTTGA TCAAACTTGA ACATCATGAT 
GAGGCAATTA CAGCTGTAAC AGAGGCAAAA GAGACAGAGT CAGTAGAAAG TGTGACTCCT 
TTAATGGACA TTTCTTCAGT CGTGAACAAG CAAGTGGCAA GAAAGTTAGG AGATAAGCAA 
GATGCAAAAC AGACCCAGTG CACATCAAAA GTAACCAAAG ACATTCAAGA AAGCGATGAG 
ACAGTCAAAG TTCCACAGGT TGAAAGTAGG CTTCATCACC AAGTCTCCAA TGGTTCCCCA 
GACATCCGAT CAAGGAATGA ACCTGAAACA GAAACTAAAC CAAAGGTTAG GAATACTACA 
GGAGGAAAGA AGAAAGGGAA GCAGCAAAGA ATCAAACAAC ATGTCTTTCA GATGAGTACC 
CTCGGTGCTC TGATGTCTCC CAAAGAACTT GATGTAGCTC TCATCAGCAA TCCAAATACC 
AGCTCGTGTG ATGGTGATTT GGACACTAAT GATACTGACA GAGAAAAAGT TGAAAGATTT 
CCTTTGTGTA ACAAGATAAA TGTGACACAT CAAGTTCATC ATGCAAAAAA TGGTTATGAA 
GAAAGCCAAA TTCCAGAATC GGGTGAAAGA GAAACCTTGA AGGACAAACA AGTGAGAACA 
TCAGTTCAGA AAAGTCTTTC CCTAGAAAAT GACAGTTCTG TGTCATCATT AGATGACCCT 
GTGTGGGCAA TTTTCAAAAA TTTCATTCCA GCATGTCTTC CATCAGAAGT TGGTAGAAAG 
GACGCCACAC TGTCAGATCA GTCTTCAAGC AGTTTGTGTG AGAGTAACAC AAGCACTTGT 
AGCTCTTCTT CAAGTGAAAC TGATAGTGGA AGCAGCGGTA CCAATGGAGA AGAAGAGGAA 
GACGGCAAGG AGGCATCTGA GAGCAACCGC AAGAATAAGT GGAAAGGAAA GGAACCTGCA 
AGAAATGGGA GTGGAAAGCA CCACATGGGG TTAAATCAAG CCAAAGCAAA GGAGAAGAAT 
CTTTCTAAAG CTAATAGCAG CTACAATGAA GAAAAGCACT ACTGTGAAGG ACAAAATAAA 
TATCCACAAT GTCCAGATGA TCCTAGTTGG ACAACTGAAC AATTTCCTAA TCAACCAAGC 
TGTAATGTAG AGGGTTGGGA GTATGATGGA AGCTATGATT ACTATGCTGC TGCTCCAATA 
CCTGGAGCTG CATACCATGG AAGGCCATAC TCAAGTTACT CGCCTCAGGG ATACACAAAT 
AGTTATTGGA ATGACTATTA CCATGGATAT GCCCAACAAC CTTATACTGG GTACCCCTAT 
TTCTCTTCTT ATGATCCTCC CCATTATTCT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC588813 ( XM_788477.4 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_793570.3 Strongylocentrotus purpuratus probable ATP-dependent RNA helicase DDX20 (LOC588813), mRNA.

Price & Availability↑

CloneID	OSd41884
Clone ID Related Accession (Same CDS sequence)	XM_788477.4
Accession Version	XM_788477.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3573bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569427200000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	probable ATP-dependent RNA helicase DDX20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_022145542.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 27, 2019 this sequence version replaced XM_788477.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Strongylocentrotus purpuratus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACGC AGACCGGCCA AGCAAGGATG GGAGCTCCGG GTTCCTTGAG AACAGCCCAT 
GCTATCAATG AAAGGGAGAG AACCAGTGAT GTTTTGACTG AAGAGAACAG CAATGTTGAC 
TTCTCATCTA TGTTCCTTTC CGAACCCGTG ACGGCCGGAT TACAGAAAGT TGGTTTTGAG 
AAACCTTCAC CTATTCAATT AAAGGCAATA CCACTTGGGA GATGCGGTTT AGACTTGATT 
GTCCAGGCTA AGTCTGGCAC AGGGAAGACG TGTGTGTTCA GTGTCATAGC TCTAGAGGGA 
ATAGATCTCA CCAATCCATC TACTCAGGTT CTGATTCTAG CCCCCACGAG AGAGATTGCA 
GTCCAGATTC AAGACACTAT TCGAGCTATA GGATGTGAGA TGGAGGGACT ACGTTCTCAT 
GTGTTTATCG GAGGTACTCT ATTTGGACCA GACAGGCAGA AGTTGAAGAA GTGTCACATA 
GCTGTTGGTA CACCAGGACG TATCAAACAG CTAATTGAGT ATGAAGTATT GAAGACCGGA 
ACTATCAGGC TTTTTGTCTT GGATGAAGCA GACAAACTAC TTGATGATAC ATTTCAGGAG 
CAAGTCAATT GGATTTACAA CCACCTTTCA GATAACAAGC AGATGTTAGC ACTCTCCGCA 
ACCTACCCCG AGTACCTTGC CAAACATCTC ACTAAGTATA TGAGAGAACC TATGTTTGTT 
CGACTCAATC CAAAGGATCT CGCATTAAGA GGCATCAAGC AGTTGTATGT GGAGTTACCT 
GGCCACTCTC TTCCTAACAA AGCATTTGAG ATCAAGGTTA TCAAACTGCT GAAAATCCTG 
ACTCAAGTAT CCTTCAATCA ATGTCTCATC TTCTCCAACT TACAGACAAG GGCTCAGAAT 
CTATGTGACA TCCTCTGTGA CTCTGGTTGG CCTAGTGAGT GTATATCAGG TGCACAGGAG 
CAGTCTCAAC GACTCAGTGC TATGGCCAAG CTAAAGAAAT TCCAGTGTCG GATCCTCATC 
TCTACGGATC TAACTGCCAG AGGTATCGAT GCTGAGAATG TCAACCTGAT CATCAACCTA 
GACATTCCAA CCGACACCAA GACCTACCTT CATCGCATTG GGAGGGCGGG GCGCTTCGGT 
ACCCATGGAG CTGCTATCAG CCTTGCTTCC AAGGGTAGGG AGATGGAACT ACTCAGGAAT 
ATCCAGCGTC AGTGTCGAGT GACAATGACA GAGCTACCTG ATCCTCCTCC ATCTTATTTG 
CTGAAGCAGG ACCACAACTC AACGGGACTT CAACAGGTTT CTATTACACC ACAGCCTCTC 
CTCCCTGCAG CCAAGATGGA TTCTACAAGA AATACCACCA CAGATGATCT GGAAAACAAT 
GCTACTGCTG AAACATCAGA TGATGCTTTG TTGACTTCCT CAGTTGTGAA TAAAGAAGAA 
GACCCCATGC CAAAATCTGT TATCGCAAAT GGGACGGATG AACAACATTC ACTCCATATG 
GGAATCAATG GGAGTTGTCA CAAGATGGAA GATAGTGCTG ATAGTTTAGC GGATAGGAAG 
ACAACAAAGA TTGTGGAAAG TATGGTGGAC CAAAATCCTA TACCAAAGAC TAGAAGAGGA 
GTCCATGAAC CACAGAAAGT TGATGCATGT ATCCAAACTG TGCAGGAAGA TGTTGTTCAA 
CCAAAGGCCA AGACATCACA GATAAGATAC GTTACTGGAA GTGGGGATCA CAAAGACAGA 
CCGACATTAC CGATTGGCTT TGAAGATGCC CCAGAAGTGA GGAAGCTCAG TCTTCCTCAA 
GTGTCCCATA TTAAAAGAGG AGATGGATCA AGAAGAGCTC GTACCTGGTA TAAAGGTAAA 
GAATCGTTTG AAAAGTTCAT GAAGGGTCAA ACAGTGGAGA CTAAAGGTCA AGGGTCAGAA 
GATGACAGGG TAGACAAGGA GACGGATGTC ATTGAGCAGA CAGTGAAAAA TGACAAGGAA 
CTGTCGGTTG AAACATCACA GTTAGAAGGT GAAGAAAATG ATGCAGATGT GTCTTCTAGA 
AACTCTCATG TCACATCAAG CAAAGATCTC AATATTGGTG ATGACTATCA GAACAAATAC 
AAAGATGAAG TCTACAATGG GAGTGGAAAT GCAGATCGTG AAATTGCGCA GAAAAGGATC 
TCTGGCTTAA CACCCATCCA AGAGATAGAG AGGTACAGGG GTCTACTCTG TGATACATTT 
ATACGCAATC ACTTAGGAAG GGGAGACAAC TCTACTTTGA TCAAACTTGA ACATCATGAT 
GAGGCAATTA CAGCTGTAAC AGAGGCAAAA GAGACAGAGT CAGTAGAAAG TGTGACTCCT 
TTAATGGACA TTTCTTCAGT CGTGAACAAG CAAGTGGCAA GAAAGTTAGG AGATAAGCAA 
GATGCAAAAC AGACCCAGTG CACATCAAAA GTAACCAAAG ACATTCAAGA AAGCGATGAG 
ACAGTCAAAG TTCCACAGGT TGAAAGTAGG CTTCATCACC AAGTCTCCAA TGGTTCCCCA 
GACATCCGAT CAAGGAATGA ACCTGAAACA GAAACTAAAC CAAAGGTTAG GAATACTACA 
GGAGGAAAGA AGAAAGGGAA GCAGCAAAGA ATCAAACAAC ATGTCTTCCA GATGAGTACC 
CTCGGTGCTC TGATGTCTCC CAAAGAACTT GATGTAGCTC TCATCAGCAA TCCAAATACC 
AGCTTGTGTG ATGGTGATTT GGACACTAAT GATACTGACA GAGAAAAAGT TGAAAGATTT 
CCTTTGTGTA ACAAGATAAA TGTGACACAT CAAGTTCATC ATACAAAAAA TGGTTATGAA 
GAAAGCCAAA TTCCAGAATT GGGTGAAAGA GAAACCTTGA AGGACAAACA AGTGAGAACA 
TCAGTTCAGA AAAGTCTTTC CCTAGAAAAT GACAGTTCTG TGTCATCATT AGATGACCCT 
GTGTGGGCAA TTTTCAAAAA TTTCATTCCA GCATGTCTTC CATCAGAAGT TGGTAGAAAG 
GACGCCACAC TGTCAGATCA GTCTTCAAGC AGTTTGTGTG AGAGTAACAC AAGCACTTGT 
AGCTCTTCTT CAAGTGAAAC TGATAGTGGA AGCAGCGGTA CCAATGGAGA AGAAGAGGAA 
GACGGCAAGG AGGCATCTGA GAGCAACCGC AAGAATAAGT GGAAAGGAAA GGAACCTGCA 
AGAAATGGGA GTGGAAAGCA CCACATGGGG TTAAATCAAG CCAAAGCAAA GGAGAAGAAT 
CTTTCTAAAG CTAATAGCTG CTACAATGAA GAAAATCACT ACTGTGAAGG ACAAAATAAA 
TATCCACAAT GTCCAGATGA TCCTAGCTGG ACAACTGAAC AATTTCCCAA TCAACCAAGC 
TTTAATGTAG AGGGTTGGGA GTATGATGGA AGCTATGATT ACTATGCTGC TGCTCCAATA 
CCTGGAGCTG CATACCATGG AAGGCCATAC TCAAGTTACT CGCCTCAGGG ATACACAAAT 
AGTTATTGGA ATGACTATTA CCATGGATAT GCCCAACAAC CTTATGCTGG GTACCCCTAT 
TTCTCTTCTT GTGATCCTCC CCATTATTCT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_793570.3
CDS	261..3833
Translation	METQTGQARMGAPGSLRTAHAINERERTSDVLTEENSNVDFSSMFLSEPVTAGLQKVGFEKPSPIQLKAIPLGRCGLDLIVQAKSGTGKTCVFSVIALEGIDLTNPSTQVLILAPTREIAVQIQDTIRAIGCEMEGLRSHVFIGGTLFGPDRQKLKKCHIAVGTPGRIKQLIEYEVLKTGTIRLFVLDEADKLLDDTFQEQVNWIYNHLSDNKQMLALSATYPEYLAKHLTKYMREPMFVRLNPKDLALRGIKQLYVELPGHSLPNKAFEIKVIKLLKILTQVSFNQCLIFSNLQTRAQNLCDILCDSGWPSECISGAQEQSQRLSAMAKLKKFQCRILISTDLTARGIDAENVNLIINLDIPTDTKTYLHRIGRAGRFGTHGAAISLASKGREMELLRNIQRQCRVTMTELPDPPPSYLLKQDHNSTGLQQVSITPQPLLPAAKMDSTRNTTTDDLENNATAETSDDALLTSSVVNKEEDPMPKSVIANGTDEQHSLHMGINGSCHKMEDSADSLADRKTTKIVESMVDQNPIPKTRRGVHEPQKVDACIQTVQEDVVQPKAKTSQIRYVTGSGDHKDRPTLPIGFEDAPEVRKLSLPQVSHIKRGDGSRRARTWYKGKESFEKFMKGQTVETKGQGSEDDRVDKETDVIEQTVKNDKELSVETSQLEGEENDADVSSRNSHVTSSKDLNIGDDYQNKYKDEVYNGSGNADREIAQKRISGLTPIQEIERYRGLLCDTFIRNHLGRGDNSTLIKLEHHDEAITAVTEAKETESVESVTPLMDISSVVNKQVARKLGDKQDAKQTQCTSKVTKDIQESDETVKVPQVESRLHHQVSNGSPDIRSRNEPETETKPKVRNTTGGKKKGKQQRIKQHVFQMSTLGALMSPKELDVALISNPNTSLCDGDLDTNDTDREKVERFPLCNKINVTHQVHHTKNGYEESQIPELGERETLKDKQVRTSVQKSLSLENDSSVSSLDDPVWAIFKNFIPACLPSEVGRKDATLSDQSSSSLCESNTSTCSSSSSETDSGSSGTNGEEEEDGKEASESNRKNKWKGKEPARNGSGKHHMGLNQAKAKEKNLSKANSCYNEENHYCEGQNKYPQCPDDPSWTTEQFPNQPSFNVEGWEYDGSYDYYAAAPIPGAAYHGRPYSSYSPQGYTNSYWNDYYHGYAQQPYAGYPYFSSCDPPHYS

Target ORF information:

RefSeq Version	XM_788477.4
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus probable ATP-dependent RNA helicase DDX20 (LOC588813), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_788477.4

ORF Insert Sequence:

ATGGAGACGC AGACCGGCCA AGCAAGGATG GGAGCTCCGG GTTCCTTGAG AACAGCCCAT 
GCTATCAATG AAAGGGAGAG AACCAGTGAT GTTTTGACTG AAGAGAACAG CAATGTTGAC 
TTCTCATCTA TGTTCCTTTC CGAACCCGTG ACGGCCGGAT TACAGAAAGT TGGTTTTGAG 
AAACCTTCAC CTATTCAATT AAAGGCAATA CCACTTGGGA GATGCGGTTT AGACTTGATT 
GTCCAGGCTA AGTCTGGCAC AGGGAAGACG TGTGTGTTCA GTGTCATAGC TCTAGAGGGA 
ATAGATCTCA CCAATCCATC TACTCAGGTT CTGATTCTAG CCCCCACGAG AGAGATTGCA 
GTCCAGATTC AAGACACTAT TCGAGCTATA GGATGTGAGA TGGAGGGACT ACGTTCTCAT 
GTGTTTATCG GAGGTACTCT ATTTGGACCA GACAGGCAGA AGTTGAAGAA GTGTCACATA 
GCTGTTGGTA CACCAGGACG TATCAAACAG CTAATTGAGT ATGAAGTATT GAAGACCGGA 
ACTATCAGGC TTTTTGTCTT GGATGAAGCA GACAAACTAC TTGATGATAC ATTTCAGGAG 
CAAGTCAATT GGATTTACAA CCACCTTTCA GATAACAAGC AGATGTTAGC ACTCTCCGCA 
ACCTACCCCG AGTACCTTGC CAAACATCTC ACTAAGTATA TGAGAGAACC TATGTTTGTT 
CGACTCAATC CAAAGGATCT CGCATTAAGA GGCATCAAGC AGTTGTATGT GGAGTTACCT 
GGCCACTCTC TTCCTAACAA AGCATTTGAG ATCAAGGTTA TCAAACTGCT GAAAATCCTG 
ACTCAAGTAT CCTTCAATCA ATGTCTCATC TTCTCCAACT TACAGACAAG GGCTCAGAAT 
CTATGTGACA TCCTCTGTGA CTCTGGTTGG CCTAGTGAGT GTATATCAGG TGCACAGGAG 
CAGTCTCAAC GACTCAGTGC TATGGCCAAG CTAAAGAAAT TCCAGTGTCG GATCCTCATC 
TCTACGGATC TAACTGCCAG AGGTATCGAT GCTGAGAATG TCAACCTGAT CATCAACCTA 
GACATTCCAA CCGACACCAA GACCTACCTT CATCGCATTG GGAGGGCGGG GCGCTTCGGT 
ACCCATGGAG CTGCTATCAG CCTTGCTTCC AAGGGTAGGG AGATGGAACT ACTCAGGAAT 
ATCCAGCGTC AGTGTCGAGT GACAATGACA GAGCTACCTG ATCCTCCTCC ATCTTATTTG 
CTGAAGCAGG ACCACAACTC AACGGGACTT CAACAGGTTT CTATTACACC ACAGCCTCTC 
CTCCCTGCAG CCAAGATGGA TTCTACAAGA AATACCACCA CAGATGATCT GGAAAACAAT 
GCTACTGCTG AAACATCAGA TGATGCTTTG TTGACTTCCT CAGTTGTGAA TAAAGAAGAA 
GACCCCATGC CAAAATCTGT TATCGCAAAT GGGACGGATG AACAACATTC ACTCCATATG 
GGAATCAATG GGAGTTGTCA CAAGATGGAA GATAGTGCTG ATAGTTTAGC GGATAGGAAG 
ACAACAAAGA TTGTGGAAAG TATGGTGGAC CAAAATCCTA TACCAAAGAC TAGAAGAGGA 
GTCCATGAAC CACAGAAAGT TGATGCATGT ATCCAAACTG TGCAGGAAGA TGTTGTTCAA 
CCAAAGGCCA AGACATCACA GATAAGATAC GTTACTGGAA GTGGGGATCA CAAAGACAGA 
CCGACATTAC CGATTGGCTT TGAAGATGCC CCAGAAGTGA GGAAGCTCAG TCTTCCTCAA 
GTGTCCCATA TTAAAAGAGG AGATGGATCA AGAAGAGCTC GTACCTGGTA TAAAGGTAAA 
GAATCGTTTG AAAAGTTCAT GAAGGGTCAA ACAGTGGAGA CTAAAGGTCA AGGGTCAGAA 
GATGACAGGG TAGACAAGGA GACGGATGTC ATTGAGCAGA CAGTGAAAAA TGACAAGGAA 
CTGTCGGTTG AAACATCACA GTTAGAAGGT GAAGAAAATG ATGCAGATGT GTCTTCTAGA 
AACTCTCATG TCACATCAAG CAAAGATCTC AATATTGGTG ATGACTATCA GAACAAATAC 
AAAGATGAAG TCTACAATGG GAGTGGAAAT GCAGATCGTG AAATTGCGCA GAAAAGGATC 
TCTGGCTTAA CACCCATCCA AGAGATAGAG AGGTACAGGG GTCTACTCTG TGATACATTT 
ATACGCAATC ACTTAGGAAG GGGAGACAAC TCTACTTTGA TCAAACTTGA ACATCATGAT 
GAGGCAATTA CAGCTGTAAC AGAGGCAAAA GAGACAGAGT CAGTAGAAAG TGTGACTCCT 
TTAATGGACA TTTCTTCAGT CGTGAACAAG CAAGTGGCAA GAAAGTTAGG AGATAAGCAA 
GATGCAAAAC AGACCCAGTG CACATCAAAA GTAACCAAAG ACATTCAAGA AAGCGATGAG 
ACAGTCAAAG TTCCACAGGT TGAAAGTAGG CTTCATCACC AAGTCTCCAA TGGTTCCCCA 
GACATCCGAT CAAGGAATGA ACCTGAAACA GAAACTAAAC CAAAGGTTAG GAATACTACA 
GGAGGAAAGA AGAAAGGGAA GCAGCAAAGA ATCAAACAAC ATGTCTTCCA GATGAGTACC 
CTCGGTGCTC TGATGTCTCC CAAAGAACTT GATGTAGCTC TCATCAGCAA TCCAAATACC 
AGCTTGTGTG ATGGTGATTT GGACACTAAT GATACTGACA GAGAAAAAGT TGAAAGATTT 
CCTTTGTGTA ACAAGATAAA TGTGACACAT CAAGTTCATC ATACAAAAAA TGGTTATGAA 
GAAAGCCAAA TTCCAGAATT GGGTGAAAGA GAAACCTTGA AGGACAAACA AGTGAGAACA 
TCAGTTCAGA AAAGTCTTTC CCTAGAAAAT GACAGTTCTG TGTCATCATT AGATGACCCT 
GTGTGGGCAA TTTTCAAAAA TTTCATTCCA GCATGTCTTC CATCAGAAGT TGGTAGAAAG 
GACGCCACAC TGTCAGATCA GTCTTCAAGC AGTTTGTGTG AGAGTAACAC AAGCACTTGT 
AGCTCTTCTT CAAGTGAAAC TGATAGTGGA AGCAGCGGTA CCAATGGAGA AGAAGAGGAA 
GACGGCAAGG AGGCATCTGA GAGCAACCGC AAGAATAAGT GGAAAGGAAA GGAACCTGCA 
AGAAATGGGA GTGGAAAGCA CCACATGGGG TTAAATCAAG CCAAAGCAAA GGAGAAGAAT 
CTTTCTAAAG CTAATAGCTG CTACAATGAA GAAAATCACT ACTGTGAAGG ACAAAATAAA 
TATCCACAAT GTCCAGATGA TCCTAGCTGG ACAACTGAAC AATTTCCCAA TCAACCAAGC 
TTTAATGTAG AGGGTTGGGA GTATGATGGA AGCTATGATT ACTATGCTGC TGCTCCAATA 
CCTGGAGCTG CATACCATGG AAGGCCATAC TCAAGTTACT CGCCTCAGGG ATACACAAAT 
AGTTATTGGA ATGACTATTA CCATGGATAT GCCCAACAAC CTTATGCTGG GTACCCCTAT 
TTCTCTTCTT GTGATCCTCC CCATTATTCT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC588813 cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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