LOC579112 cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC579112
Entrez Gene ID	579112
Full Name	uncharacterized LOC579112
Gene Type	protein-coding
Organism	Strongylocentrotus purpuratus(purple sea urchin)

mRNA and Protein(s)

mRNA	Protein	Name
XM_779246.5	XP_784339.3	uncharacterized protein LOC579112
XM_779246.4	XP_784339.2	uncharacterized protein LOC579112

Pathways from BioSystems
Gene Ontology

The following LOC579112 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC579112 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSd12283	XM_779246.4 Latest version!	Strongylocentrotus purpuratus uncharacterized LOC579112 (LOC579112), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OSd69935	XM_779246.5 Latest version!	Strongylocentrotus purpuratus uncharacterized LOC579112 (LOC579112), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC579112 ( XM_779246.4 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_784339.2 Strongylocentrotus purpuratus uncharacterized LOC579112 (LOC579112), mRNA.

Price & Availability↑

CloneID	OSd12283
Clone ID Related Accession (Same CDS sequence)	XM_779246.4
Accession Version	XM_779246.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1427040000000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	uncharacterized protein LOC579112
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011995677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 24, 2015 this sequence version replaced XM_779246.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Strongylocentrotus purpuratus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACACTC CTCCCGAAGT GCTGAGTATG GGATCACCAG AGGAGTACCA TGACCCTAAC 
CATGACCCTG GACACCCCTT TGACCTCTCA GGTCAGCAGC AGGCATGGAA AGACGGGAGC 
GAATCCACAG CCGTCCTGCA ACCAAATGGA GGTTATGATG ACAAGTTAGA AGTGAAAGAA 
GAAGAGGAAG ATGAGGAGGA GGAAATAGAA GAGAGGGGAA ACGAGGAATA TGAAAAGTTG 
AACGAGGAAG AGGAGGAAAT CAGCTCGCCA GTTTCATCTC TTTTATCATC TGGAGATGAG 
GCTGATGATG ATGAAGAAGA AAATGAGGAT GATCATTTAG ACAGAGAAGT TTTGACACGT 
GATCACGCTC AGAGCAGAGG CCGCCATTCT TTTGAATCTG AGAGCGAAGA ACAGCATTCA 
CCAAGAGGAC GACCAAACTT TGAACTGAGT GAACACATAG ATGCTAGGAG TAGAGAGGAT 
GATGAGGATG TGGGGATTCA TGAAGAAAGT CACACACCTC ACAATGATGA GAATGATCTT 
GAGGGCACTC CTAGAGAGAT GACCCAAAGG GGTTTTGAAG AGGAAGATGA AGACTACGAT 
GAAGGAGAGG ATGAAGACGA TGAGGAAGAT AGAATAAAGG AGACGCAAAA GCTTGTTGCC 
AAGATCGAGC ACGACATCCA GGGTCTGCGG TTCCAGGCCA AACAACGTGG TCACCTCTTG 
TCAGATCTCC ACAGATATGG TGCACAAAAC CAGCTGCGAA GAAAGGAGCA GGAACGTCTG 
AAGCAGTCCT CCTCCTCTGT AGAGCAAACA AGTGATCTCC TCACGGCAGC ACCACAACCA 
AAGCCTAGTG ATGAAGGCAA GATAATAGAA AGGCCAGTGT CGATCAAACT GCGCCTAAGA 
ACTCGGAGCA CCTCACCCAA GGCACAGCAG GAACGGTATG CCCTGCCCCC TATTGTGAAG 
GCCAAACCAG GCAAAGTGAA GATGCGTGTT ATGAGGATCG GCAAGCAGCT TGGACGATGG 
GATGCATGGA AGGAGAGCCT AGGGGTCAAA GCAGACAGGG ACCTAGCTAA GATCATGATG 
GACCACATGG ACAGAACCTA TTTCCAGCAG TTTATTTCTC CCAAACGAGA TGTCAAAAAA 
GGTCAGAAAC GCAAGAAGAA AGATAAAGAG GGCCACAAAC ATGAACGGAA GCCCAAGAAA 
CCCAAGCAAG TGAAAGTCAT TGATGAACCT GAAGAATGGG CAGATGGATA TCTTGATGAT 
GAACTGGATG AATTTGGTGA TCCTGTGGAA GAAAAGGATG ACTACGACTA TGATCCATCT 
CATGAACATG AAGGCTTTGG AGCATATAAG CCATCGAATC CAGTAAGAAA CAAGAGGAAG 
TCTCAAAGAC CACGGAAGCT GATGGATGTG AACTCTAGCC CCAGACAATC CAAGACAAGC 
ACCAAGCTTC CACGTCAGCC CAAGAGATCA CCCAAACCTC AGAAGAAAGG AGGCCAAGAA 
GATGGAGAGG TGCCCTTAGT ACAGGAGAGG AGGAGGGGAC GCAAACCTAA ACCAAAGCTA 
GTAGTCATCA AGACTAACAA GAAGAATAAA GCTCCTAGGA TACACAGAGG AGGAAGGAGA 
CCATCTCTGA TGAGTACTTT AAAAAACCTT GACTGGAATG CTCTTCCAAG ACGCTATGAA 
TGCAAGATCT GTTGCCTACG CTTTGATGGA ATCACTGCCT TCAAGTCCCA CCTTGAATCA 
ACTCATACTT CTGAATATAT GAAACCAAAG GAACCGGACA ATCATAATCC TGCTGGAGAT 
GAGAATTCCA ATGATGGGCA GGAGGCAGGG AAACACTTCC ATGGTTCCGA GAAGCAGTAC 
CAGGACTCTG AGAAGCAATA CGAAGACCTG GGCCATCTGC CTGACCCACA TTCATCTATG 
CAGGAAAGTG AACAGCGCAG AATGGCAGAG AGCGAGATGT ATCGGACAAT GGCTTATGGT 
CCGGATATGA TGGACGCAAG ACGCTTTGAG ATGATGCCAC CCTTTAAGAG GCATCTTCAT 
GAGAGCTATA CAAATGAGTG GGGTAAAATG CAAGGAAACA TGTACAGTCG ACCTTTAGCC 
TCAGGTCAGA TCATGCATGG GGAGCAGCCC TGGGGACATA ATCGGGGGTA TGATGCCATC 
CCTGCATACA GAAAGCATTA TGAGGATGGG CGAGAGTGGG GTAAGAGTGG GGGACAGTAC 
CCACCTGTTA TTACATCACT TGGACAGAAC ATGCAAATAG GTATTGATGG AGGAGAAGGA 
GGTGGTCATA GACAGGAGCA GCATGGGGAA GGGTACAACA TGCCAGGGGG TAGTATGGTG 
GGGATTGACC ATGCCCCCTC CCAAGATAAC ACCTATGTTG ATATGAGTAT GAGACACAGT 
GAGGAGAATA CAAACCGGGA TCATGAGGGT GTGTCACAGG AGAGGATGGA GAAGGGTGAG 
GAAGGAGAAA ATGAAGAAAG AGAAGTAAGT GAACCTCCAC CTGAGACTGA TACAAGGTCA 
AATGAAGGCA GCAAAGAGGC TGAAGATGAG ACCAATGAGC AAGAAGAGGA TGAAGAACAG 
AAGAGGGATG AAGATGAGAC TGGAAAAAAA AAGAAGAGAA AGAAAACAAA ACCTTCTAAG 
GAGTGGGAGG AAATAGATAG GTGGGCCGTC ACATTTGTAC ACTGTCCGCA GTGTAATGAC 
TACATTCGCT TTCGACCTGG TCAGAAGAAC TTCCATTTTG CTGTCCATGC AGAACGTCGT 
CTTTGCCAAG AATGTGGCAA GATGTTTACT AAAGAGGCTT TCAGGGCTCA TGTCCGAGTT 
CACCAGGCTA AGAGAATGGG AGAGGTCCTC AAGCACATCT GTGAAATCTG TGGCAAAGCC 
TACCGCTTCA AGTGCAGCTT GAACTCTCAC ATGCGCTGCC ACACAGGAGT CCGGGACTTT 
GACTGTAAGA TATGCGGTAA GAAATTCCTC TCGGCCCATG GGCTTGAGAA ACATGAGCTG 
GTCCATCGTA AAGTAAAGCC CCACGTGTGC CCTGATTGTG GGCGTGGCTT CACCCAGTTC 
CATAACATGC TTGCCCATCG CAGGCAGCAC ACGGGTGAGA AACCTTACCG CTGCAACTTC 
TGTGACCGCT CTTTCACCCA TAATGTCAGC CTGAAGAATC ATAAGAAGAA AGAGCATGGG 
GTTGACCTAT GGCAAGGGAC GGGTGGGAAA CCCCCCTCCT CAAGTCGTTC ACGGTCCAAG 
TCGTCAAGAA GTGCCCAGGC TGTTGTTCCT GTCAGTCCTC CTGTACCCCA GCAGCCACCT 
GCACAGCCAA AGCCATCAGT GCCAAGTGAA AGAGAGTTAA TGCATACATT AGATCCTGCT 
CAGCTATATA ACATGATGCC CACTCTGGAT TATGGGCATT ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_784339.2
CDS	114..3518
Translation	MNTPPEVLSMGSPEEYHDPNHDPGHPFDLSGQQQAWKDGSESTAVLQPNGGYDDKLEVKEEEEDEEEEIEERGNEEYEKLNEEEEEISSPVSSLLSSGDEADDDEEENEDDHLDREVLTRDHAQSRGRHSFESESEEQHSPRGRPNFELSEHIDARSREDDEDVGIHEESHTPHNDENDLEGTPREMTQRGFEEEDEDYDEGEDEDDEEDRIKETQKLVAKIEHDIQGLRFQAKQRGHLLSDLHRYGAQNQLRRKEQERLKQSSSSVEQTSDLLTAAPQPKPSDEGKIIERPVSIKLRLRTRSTSPKAQQERYALPPIVKAKPGKVKMRVMRIGKQLGRWDAWKESLGVKADRDLAKIMMDHMDRTYFQQFISPKRDVKKGQKRKKKDKEGHKHERKPKKPKQVKVIDEPEEWADGYLDDELDEFGDPVEEKDDYDYDPSHEHEGFGAYKPSNPVRNKRKSQRPRKLMDVNSSPRQSKTSTKLPRQPKRSPKPQKKGGQEDGEVPLVQERRRGRKPKPKLVVIKTNKKNKAPRIHRGGRRPSLMSTLKNLDWNALPRRYECKICCLRFDGITAFKSHLESTHTSEYMKPKEPDNHNPAGDENSNDGQEAGKHFHGSEKQYQDSEKQYEDLGHLPDPHSSMQESEQRRMAESEMYRTMAYGPDMMDARRFEMMPPFKRHLHESYTNEWGKMQGNMYSRPLASGQIMHGEQPWGHNRGYDAIPAYRKHYEDGREWGKSGGQYPPVITSLGQNMQIGIDGGEGGGHRQEQHGEGYNMPGGSMVGIDHAPSQDNTYVDMSMRHSEENTNRDHEGVSQERMEKGEEGENEEREVSEPPPETDTRSNEGSKEAEDETNEQEEDEEQKRDEDETGKKKKRKKTKPSKEWEEIDRWAVTFVHCPQCNDYIRFRPGQKNFHFAVHAERRLCQECGKMFTKEAFRAHVRVHQAKRMGEVLKHICEICGKAYRFKCSLNSHMRCHTGVRDFDCKICGKKFLSAHGLEKHELVHRKVKPHVCPDCGRGFTQFHNMLAHRRQHTGEKPYRCNFCDRSFTHNVSLKNHKKKEHGVDLWQGTGGKPPSSSRSRSKSSRSAQAVVPVSPPVPQQPPAQPKPSVPSERELMHTLDPAQLYNMMPTLDYGHY

Target ORF information:

RefSeq Version	XM_779246.4
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus uncharacterized LOC579112 (LOC579112), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_779246.4

ORF Insert Sequence:

ATGAACACTC CTCCCGAAGT GCTGAGTATG GGATCACCAG AGGAGTACCA TGACCCTAAC 
CATGACCCTG GACACCCCTT TGACCTCTCA GGTCAGCAGC AGGCATGGAA AGACGGGAGC 
GAATCCACAG CCGTCCTGCA ACCAAATGGA GGTTATGATG ACAAGTTAGA AGTGAAAGAA 
GAAGAGGAAG ATGAGGAGGA GGAAATAGAA GAGAGGGGAA ACGAGGAATA TGAAAAGTTG 
AACGAGGAAG AGGAGGAAAT CAGCTCGCCA GTTTCATCTC TTTTATCATC TGGAGATGAG 
GCTGATGATG ATGAAGAAGA AAATGAGGAT GATCATTTAG ACAGAGAAGT TTTGACACGT 
GATCACGCTC AGAGCAGAGG CCGCCATTCT TTTGAATCTG AGAGCGAAGA ACAGCATTCA 
CCAAGAGGAC GACCAAACTT TGAACTGAGT GAACACATAG ATGCTAGGAG TAGAGAGGAT 
GATGAGGATG TGGGGATTCA TGAAGAAAGT CACACACCTC ACAATGATGA GAATGATCTT 
GAGGGCACTC CTAGAGAGAT GACCCAAAGG GGTTTTGAAG AGGAAGATGA AGACTACGAT 
GAAGGAGAGG ATGAAGACGA TGAGGAAGAT AGAATAAAGG AGACGCAAAA GCTTGTTGCC 
AAGATCGAGC ACGACATCCA GGGTCTGCGG TTCCAGGCCA AACAACGTGG TCACCTCTTG 
TCAGATCTCC ACAGATATGG TGCACAAAAC CAGCTGCGAA GAAAGGAGCA GGAACGTCTG 
AAGCAGTCCT CCTCCTCTGT AGAGCAAACA AGTGATCTCC TCACGGCAGC ACCACAACCA 
AAGCCTAGTG ATGAAGGCAA GATAATAGAA AGGCCAGTGT CGATCAAACT GCGCCTAAGA 
ACTCGGAGCA CCTCACCCAA GGCACAGCAG GAACGGTATG CCCTGCCCCC TATTGTGAAG 
GCCAAACCAG GCAAAGTGAA GATGCGTGTT ATGAGGATCG GCAAGCAGCT TGGACGATGG 
GATGCATGGA AGGAGAGCCT AGGGGTCAAA GCAGACAGGG ACCTAGCTAA GATCATGATG 
GACCACATGG ACAGAACCTA TTTCCAGCAG TTTATTTCTC CCAAACGAGA TGTCAAAAAA 
GGTCAGAAAC GCAAGAAGAA AGATAAAGAG GGCCACAAAC ATGAACGGAA GCCCAAGAAA 
CCCAAGCAAG TGAAAGTCAT TGATGAACCT GAAGAATGGG CAGATGGATA TCTTGATGAT 
GAACTGGATG AATTTGGTGA TCCTGTGGAA GAAAAGGATG ACTACGACTA TGATCCATCT 
CATGAACATG AAGGCTTTGG AGCATATAAG CCATCGAATC CAGTAAGAAA CAAGAGGAAG 
TCTCAAAGAC CACGGAAGCT GATGGATGTG AACTCTAGCC CCAGACAATC CAAGACAAGC 
ACCAAGCTTC CACGTCAGCC CAAGAGATCA CCCAAACCTC AGAAGAAAGG AGGCCAAGAA 
GATGGAGAGG TGCCCTTAGT ACAGGAGAGG AGGAGGGGAC GCAAACCTAA ACCAAAGCTA 
GTAGTCATCA AGACTAACAA GAAGAATAAA GCTCCTAGGA TACACAGAGG AGGAAGGAGA 
CCATCTCTGA TGAGTACTTT AAAAAACCTT GACTGGAATG CTCTTCCAAG ACGCTATGAA 
TGCAAGATCT GTTGCCTACG CTTTGATGGA ATCACTGCCT TCAAGTCCCA CCTTGAATCA 
ACTCATACTT CTGAATATAT GAAACCAAAG GAACCGGACA ATCATAATCC TGCTGGAGAT 
GAGAATTCCA ATGATGGGCA GGAGGCAGGG AAACACTTCC ATGGTTCCGA GAAGCAGTAC 
CAGGACTCTG AGAAGCAATA CGAAGACCTG GGCCATCTGC CTGACCCACA TTCATCTATG 
CAGGAAAGTG AACAGCGCAG AATGGCAGAG AGCGAGATGT ATCGGACAAT GGCTTATGGT 
CCGGATATGA TGGACGCAAG ACGCTTTGAG ATGATGCCAC CCTTTAAGAG GCATCTTCAT 
GAGAGCTATA CAAATGAGTG GGGTAAAATG CAAGGAAACA TGTACAGTCG ACCTTTAGCC 
TCAGGTCAGA TCATGCATGG GGAGCAGCCC TGGGGACATA ATCGGGGGTA TGATGCCATC 
CCTGCATACA GAAAGCATTA TGAGGATGGG CGAGAGTGGG GTAAGAGTGG GGGACAGTAC 
CCACCTGTTA TTACATCACT TGGACAGAAC ATGCAAATAG GTATTGATGG AGGAGAAGGA 
GGTGGTCATA GACAGGAGCA GCATGGGGAA GGGTACAACA TGCCAGGGGG TAGTATGGTG 
GGGATTGACC ATGCCCCCTC CCAAGATAAC ACCTATGTTG ATATGAGTAT GAGACACAGT 
GAGGAGAATA CAAACCGGGA TCATGAGGGT GTGTCACAGG AGAGGATGGA GAAGGGTGAG 
GAAGGAGAAA ATGAAGAAAG AGAAGTAAGT GAACCTCCAC CTGAGACTGA TACAAGGTCA 
AATGAAGGCA GCAAAGAGGC TGAAGATGAG ACCAATGAGC AAGAAGAGGA TGAAGAACAG 
AAGAGGGATG AAGATGAGAC TGGAAAAAAA AAGAAGAGAA AGAAAACAAA ACCTTCTAAG 
GAGTGGGAGG AAATAGATAG GTGGGCCGTC ACATTTGTAC ACTGTCCGCA GTGTAATGAC 
TACATTCGCT TTCGACCTGG TCAGAAGAAC TTCCATTTTG CTGTCCATGC AGAACGTCGT 
CTTTGCCAAG AATGTGGCAA GATGTTTACT AAAGAGGCTT TCAGGGCTCA TGTCCGAGTT 
CACCAGGCTA AGAGAATGGG AGAGGTCCTC AAGCACATCT GTGAAATCTG TGGCAAAGCC 
TACCGCTTCA AGTGCAGCTT GAACTCTCAC ATGCGCTGCC ACACAGGAGT CCGGGACTTT 
GACTGTAAGA TATGCGGTAA GAAATTCCTC TCGGCCCATG GGCTTGAGAA ACATGAGCTG 
GTCCATCGTA AAGTAAAGCC CCACGTGTGC CCTGATTGTG GGCGTGGCTT CACCCAGTTC 
CATAACATGC TTGCCCATCG CAGGCAGCAC ACGGGTGAGA AACCTTACCG CTGCAACTTC 
TGTGACCGCT CTTTCACCCA TAATGTCAGC CTGAAGAATC ATAAGAAGAA AGAGCATGGG 
GTTGACCTAT GGCAAGGGAC GGGTGGGAAA CCCCCCTCCT CAAGTCGTTC ACGGTCCAAG 
TCGTCAAGAA GTGCCCAGGC TGTTGTTCCT GTCAGTCCTC CTGTACCCCA GCAGCCACCT 
GCACAGCCAA AGCCATCAGT GCCAAGTGAA AGAGAGTTAA TGCATACATT AGATCCTGCT 
CAGCTATATA ACATGATGCC CACTCTGGAT TATGGGCATT ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC579112 ( XM_779246.5 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_784339.3 Strongylocentrotus purpuratus uncharacterized LOC579112 (LOC579112), mRNA.

Price & Availability↑

CloneID	OSd69935
Clone ID Related Accession (Same CDS sequence)	XM_779246.5
Accession Version	XM_779246.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569427200000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	uncharacterized protein LOC579112
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_022145613.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 27, 2019 this sequence version replaced XM_779246.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Strongylocentrotus purpuratus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACACTC CTCCCGAAGT GCTGAGTATG GGATCACCAG AGGAGTACCA TGACCCTAAC 
CATGACCCTG GACACCCCTT TGACCTCTCA GGTCAGCAGC AGGCATGGAA AGACGGGAGC 
GAATCCACAG CCGTCCTGCA ACCAAATGGA GGTTATGATG ACAAGTTAGA AGTGAAAGAA 
GAAGAGGAAG ATGAGGAGGA GGAAATAGAA GAGAGGGGAA ACGAGGAATA TGAAAAGTTG 
AACGAGGAAG AGGAGGAAAT CAGCTCGCCA GTTTCATCTC TTTTATCATC TGGAGATGAG 
GCTGATGATG ATGAAGAAGA AAATGAGGAT GATCATTTAG ACAGAGAAGT TTTGACACGT 
GATCACGCTC AGAGCAGAGG CCGCCATTCT TTTGAATCTG AGAGTGAAGA ACAGCATTCA 
CCAAGAGGAC GACCAAACTT TGAACTGAGT GAACACATAG ATGCTAGAAG TAGAGAGGAT 
GATGAGGATG TGGGGATTCA TGAAGAAAGT CACCCAACTC ACAATGATGA TAATGATCTT 
GAGGGCACTC CTAGAGAGAT GACCCAAAGG GGTTTTGAAG AGGAAGATGA TGACTACGAT 
GAAGGAGAGG ATGAGGACGA TGAGGAAGAT AGAATAAAGG AGACGCAAAA GCTTGTTGCC 
AAGATCGAGC ACGATATCCA GGGTCTGCGG TTCCAGGCCA AACAACGCGG TCACCTCTTG 
TCAGATCTCC ACAGATATGG TGCACAAAAC CAGCTGCGAA GAAAGGAACA GGAACGTCTG 
AAGCAGTCCT CCTCCTCTGT AGAGCAAACC AGTGATCTCC TCACGGCAGC ACCACAACCA 
AAGCCTAGTG ATGAAGGCAA GATAATAGAA AGGCCAGTGT CGATCAAACT GCGCCTAAGA 
ACTCGAAGCA CCTCACCCAA GGCACAGCAG GAACGGTATG CCCTGCCCCC TATTGTGAAG 
GCCAAACCAG GCAAAGTGAA GATGCGTGTT ATGAGGATCG GCAAGCAGCT TGGACGATGG 
GATGCATGGA AGGAGAGCCT AGGGGTCAAA GCAGACAGGG ACCTAGCCAA GATCATGATG 
GACCACATGG ACAGAACCTA TTTCCAGCAG TTTATTTCTC CCAAACGAGA TGTCAAAAAA 
GGTCAGAAAC GCAAGAAGAA AGATAAAGAG GGCCACAAAC ATGAACGGAA GCCCAAGAAA 
CCCAAGCAAG TGAAAGTCAT TGATGAACCT GAAGAATGGG CAGATGGATA TCTTGATGAT 
GAACTGGATG AATTTGGTGA TCCTGTTGAA GAAAAGGATG ACTACGACTA TGATCCATCT 
CATGAACATG AAGGCTTTGG AGCATATAAG CCATCGAATC CAGTAAGAAA CAAGAGGAAG 
TCTCAAAGAC CACGGAAGCT GATGGATGTG AACTCTAGCC CCAGACAATC CAAGACAAGC 
ACCAAGCTTC CACGTCAGCC CAAGAGATCG CCCAAACCTC AGAAGAAAGG AGGCCAAGAA 
GATGGAGAGG TGCCCTTAGT ACAGGAGAGG AGGAGGGGAC GTAAACCTAA ACCAAAGCTA 
GTAGTCATCA AGACTAACAA GAAGAATAAA GCTCCTAGGA TACACAGAGG AGGAAGGAGA 
CCATCTCTGA TGAGTACTTT AAAAAACCTT GACTGGAATG CTCTTCCAAG ACGCTATGAA 
TGCAAGATCT GTTGCCTACG CTTTGATGGA ATCACTGCCT TCAAGTCCCA CCTTGAATCA 
ACTCATACTT CTGAATATAT GAAACCAAAG GAACCGGACA ATCACAATCC TGCTGGAGAT 
GAGAATTCCA ATGATGGGCA GGAGGCAGGG AAACACTTTC ATGGTTCCGA GAAGCAGTAC 
CAGGACTCTG AGAAGCAATA CGAGGACCTG GGCCATCTGC CTGACCCACA TTCATCTATG 
CAGGAAAGTG AACAGCGCAG AATGGCAGAG AGCGAGATGT ATCGGACAAT GGCTTATGGT 
CCGGATATGA TGGACGCACG ACGCTTTGAG ATGATGCCAC CCTTTAAGAG GCATCTTCAC 
GAGAGCTATA CAAATGAGTG GGGTAAAATG CAAGGAAACA TGTACAGTAG ACCTTTGGCC 
TCAGGTCAGA TCATGCATGG GGAGCAGCCC TGGGGACATA ATCGGGGGTA TGATGCCATC 
CCTGCATACA GAAAGCATTA TGAGGATGGG CGAGAGTGGG GTAAGAGTGG GGGACAGTAC 
CCACCTGTTA TTACATCACT TGGACAGAAC ATGCAAATAG GTATTGATGG AGGAGAAGGA 
GGTGGTCATA GACAGGAGCA GCATGGGGAA GGGTACAACA TACCAGGGGG TAGTATGGTG 
GGGATTGACC ATGCCCCCTC CCAAGATAAC ACCTATGTTG ATATGAGTAT GAGACACAGT 
GGGGAGAATA CAAACCGGGA TCATGAGGGT GTGTCACAGG AAAGGATGGA GAAAGGTGAG 
GAAGAAGAGA ATGAAGAAAG AGAAGTAAGT GAACCTCCAC CTGAGACTGA TACAAGGTCA 
AATGATGGCA GCAAAGAGGC TGAAGATGAG ACCAACGAGC AAGAAGGGGA TGAAGAACAG 
AAGAGGGATG AAGATGAGAC TGGGAAAAAA AAGAAGAGAA AGAAAACAAA ACCTTCTAAG 
GAGTGGGAGG AAATAGATAG GTGGGCCGTC ACATTTGTTC ACTGTCCGCA GTGTAATGAC 
TACATTCGCT TTCGACCCGG TCAGAAGAAC TTCCATTTTG CTGTCCATGC AGAACGCCGT 
CTTTGCCAAG AATGTGGCAA GATGTTTACT AAAGAGGCTT TCAGGGCTCA TGTCCGAGTT 
CACCAGGCTA AGAGAATGGG AGAGGTCCTC AAGCACATCT GTGAAATCTG TGGCAAAGCC 
TACCGCTTCA AGTGCAGCTT GAACTCTCAC ATGCGCTGCC ACACAGGAGT CCGGGACTTT 
GACTGTAAGA TATGTGGTAA GAAATTCCTC TCGGCCCATG GGCTGGAGAA ACATGAACTG 
GTCCATCGTA AAGTAAAGCC CCACGTGTGC CCTGATTGTG GGCGTGGCTT CACCCAGTTC 
CATAACATGC TTGCCCATCG CAGGCAGCAC ACCGGTGAGA AACCTTACCG CTGTAACTTC 
TGCGACCGTT CATTCACCCA TAATGTCAGC CTGAAGAATC ATAAGAAGAA AGAGCATGGG 
GTTGACCTAT GGCAAGGGAC GGGTGGGAAA CCCCCCTCCT CAAGTCGTTC ACGGTCCAAG 
TCGTCAAGAA GTGCCCAGGC TGTTGTTCCT GTCAGTCCTC CTGTACCCCA GCAGCCACCT 
GCACAGCCAA AGCCATCAGT GCCAAGTGAA AGAGAGTTAA TGCATACATT AGATCCTGCT 
CAGCTATATA ACATGATGCC CACTCTGGAT TATGGGCATT ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_784339.3
CDS	126..3530
Translation	MNTPPEVLSMGSPEEYHDPNHDPGHPFDLSGQQQAWKDGSESTAVLQPNGGYDDKLEVKEEEEDEEEEIEERGNEEYEKLNEEEEEISSPVSSLLSSGDEADDDEEENEDDHLDREVLTRDHAQSRGRHSFESESEEQHSPRGRPNFELSEHIDARSREDDEDVGIHEESHPTHNDDNDLEGTPREMTQRGFEEEDDDYDEGEDEDDEEDRIKETQKLVAKIEHDIQGLRFQAKQRGHLLSDLHRYGAQNQLRRKEQERLKQSSSSVEQTSDLLTAAPQPKPSDEGKIIERPVSIKLRLRTRSTSPKAQQERYALPPIVKAKPGKVKMRVMRIGKQLGRWDAWKESLGVKADRDLAKIMMDHMDRTYFQQFISPKRDVKKGQKRKKKDKEGHKHERKPKKPKQVKVIDEPEEWADGYLDDELDEFGDPVEEKDDYDYDPSHEHEGFGAYKPSNPVRNKRKSQRPRKLMDVNSSPRQSKTSTKLPRQPKRSPKPQKKGGQEDGEVPLVQERRRGRKPKPKLVVIKTNKKNKAPRIHRGGRRPSLMSTLKNLDWNALPRRYECKICCLRFDGITAFKSHLESTHTSEYMKPKEPDNHNPAGDENSNDGQEAGKHFHGSEKQYQDSEKQYEDLGHLPDPHSSMQESEQRRMAESEMYRTMAYGPDMMDARRFEMMPPFKRHLHESYTNEWGKMQGNMYSRPLASGQIMHGEQPWGHNRGYDAIPAYRKHYEDGREWGKSGGQYPPVITSLGQNMQIGIDGGEGGGHRQEQHGEGYNIPGGSMVGIDHAPSQDNTYVDMSMRHSGENTNRDHEGVSQERMEKGEEEENEEREVSEPPPETDTRSNDGSKEAEDETNEQEGDEEQKRDEDETGKKKKRKKTKPSKEWEEIDRWAVTFVHCPQCNDYIRFRPGQKNFHFAVHAERRLCQECGKMFTKEAFRAHVRVHQAKRMGEVLKHICEICGKAYRFKCSLNSHMRCHTGVRDFDCKICGKKFLSAHGLEKHELVHRKVKPHVCPDCGRGFTQFHNMLAHRRQHTGEKPYRCNFCDRSFTHNVSLKNHKKKEHGVDLWQGTGGKPPSSSRSRSKSSRSAQAVVPVSPPVPQQPPAQPKPSVPSERELMHTLDPAQLYNMMPTLDYGHY

Target ORF information:

RefSeq Version	XM_779246.5
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus uncharacterized LOC579112 (LOC579112), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_779246.5

ORF Insert Sequence:

ATGAACACTC CTCCCGAAGT GCTGAGTATG GGATCACCAG AGGAGTACCA TGACCCTAAC 
CATGACCCTG GACACCCCTT TGACCTCTCA GGTCAGCAGC AGGCATGGAA AGACGGGAGC 
GAATCCACAG CCGTCCTGCA ACCAAATGGA GGTTATGATG ACAAGTTAGA AGTGAAAGAA 
GAAGAGGAAG ATGAGGAGGA GGAAATAGAA GAGAGGGGAA ACGAGGAATA TGAAAAGTTG 
AACGAGGAAG AGGAGGAAAT CAGCTCGCCA GTTTCATCTC TTTTATCATC TGGAGATGAG 
GCTGATGATG ATGAAGAAGA AAATGAGGAT GATCATTTAG ACAGAGAAGT TTTGACACGT 
GATCACGCTC AGAGCAGAGG CCGCCATTCT TTTGAATCTG AGAGTGAAGA ACAGCATTCA 
CCAAGAGGAC GACCAAACTT TGAACTGAGT GAACACATAG ATGCTAGAAG TAGAGAGGAT 
GATGAGGATG TGGGGATTCA TGAAGAAAGT CACCCAACTC ACAATGATGA TAATGATCTT 
GAGGGCACTC CTAGAGAGAT GACCCAAAGG GGTTTTGAAG AGGAAGATGA TGACTACGAT 
GAAGGAGAGG ATGAGGACGA TGAGGAAGAT AGAATAAAGG AGACGCAAAA GCTTGTTGCC 
AAGATCGAGC ACGATATCCA GGGTCTGCGG TTCCAGGCCA AACAACGCGG TCACCTCTTG 
TCAGATCTCC ACAGATATGG TGCACAAAAC CAGCTGCGAA GAAAGGAACA GGAACGTCTG 
AAGCAGTCCT CCTCCTCTGT AGAGCAAACC AGTGATCTCC TCACGGCAGC ACCACAACCA 
AAGCCTAGTG ATGAAGGCAA GATAATAGAA AGGCCAGTGT CGATCAAACT GCGCCTAAGA 
ACTCGAAGCA CCTCACCCAA GGCACAGCAG GAACGGTATG CCCTGCCCCC TATTGTGAAG 
GCCAAACCAG GCAAAGTGAA GATGCGTGTT ATGAGGATCG GCAAGCAGCT TGGACGATGG 
GATGCATGGA AGGAGAGCCT AGGGGTCAAA GCAGACAGGG ACCTAGCCAA GATCATGATG 
GACCACATGG ACAGAACCTA TTTCCAGCAG TTTATTTCTC CCAAACGAGA TGTCAAAAAA 
GGTCAGAAAC GCAAGAAGAA AGATAAAGAG GGCCACAAAC ATGAACGGAA GCCCAAGAAA 
CCCAAGCAAG TGAAAGTCAT TGATGAACCT GAAGAATGGG CAGATGGATA TCTTGATGAT 
GAACTGGATG AATTTGGTGA TCCTGTTGAA GAAAAGGATG ACTACGACTA TGATCCATCT 
CATGAACATG AAGGCTTTGG AGCATATAAG CCATCGAATC CAGTAAGAAA CAAGAGGAAG 
TCTCAAAGAC CACGGAAGCT GATGGATGTG AACTCTAGCC CCAGACAATC CAAGACAAGC 
ACCAAGCTTC CACGTCAGCC CAAGAGATCG CCCAAACCTC AGAAGAAAGG AGGCCAAGAA 
GATGGAGAGG TGCCCTTAGT ACAGGAGAGG AGGAGGGGAC GTAAACCTAA ACCAAAGCTA 
GTAGTCATCA AGACTAACAA GAAGAATAAA GCTCCTAGGA TACACAGAGG AGGAAGGAGA 
CCATCTCTGA TGAGTACTTT AAAAAACCTT GACTGGAATG CTCTTCCAAG ACGCTATGAA 
TGCAAGATCT GTTGCCTACG CTTTGATGGA ATCACTGCCT TCAAGTCCCA CCTTGAATCA 
ACTCATACTT CTGAATATAT GAAACCAAAG GAACCGGACA ATCACAATCC TGCTGGAGAT 
GAGAATTCCA ATGATGGGCA GGAGGCAGGG AAACACTTTC ATGGTTCCGA GAAGCAGTAC 
CAGGACTCTG AGAAGCAATA CGAGGACCTG GGCCATCTGC CTGACCCACA TTCATCTATG 
CAGGAAAGTG AACAGCGCAG AATGGCAGAG AGCGAGATGT ATCGGACAAT GGCTTATGGT 
CCGGATATGA TGGACGCACG ACGCTTTGAG ATGATGCCAC CCTTTAAGAG GCATCTTCAC 
GAGAGCTATA CAAATGAGTG GGGTAAAATG CAAGGAAACA TGTACAGTAG ACCTTTGGCC 
TCAGGTCAGA TCATGCATGG GGAGCAGCCC TGGGGACATA ATCGGGGGTA TGATGCCATC 
CCTGCATACA GAAAGCATTA TGAGGATGGG CGAGAGTGGG GTAAGAGTGG GGGACAGTAC 
CCACCTGTTA TTACATCACT TGGACAGAAC ATGCAAATAG GTATTGATGG AGGAGAAGGA 
GGTGGTCATA GACAGGAGCA GCATGGGGAA GGGTACAACA TACCAGGGGG TAGTATGGTG 
GGGATTGACC ATGCCCCCTC CCAAGATAAC ACCTATGTTG ATATGAGTAT GAGACACAGT 
GGGGAGAATA CAAACCGGGA TCATGAGGGT GTGTCACAGG AAAGGATGGA GAAAGGTGAG 
GAAGAAGAGA ATGAAGAAAG AGAAGTAAGT GAACCTCCAC CTGAGACTGA TACAAGGTCA 
AATGATGGCA GCAAAGAGGC TGAAGATGAG ACCAACGAGC AAGAAGGGGA TGAAGAACAG 
AAGAGGGATG AAGATGAGAC TGGGAAAAAA AAGAAGAGAA AGAAAACAAA ACCTTCTAAG 
GAGTGGGAGG AAATAGATAG GTGGGCCGTC ACATTTGTTC ACTGTCCGCA GTGTAATGAC 
TACATTCGCT TTCGACCCGG TCAGAAGAAC TTCCATTTTG CTGTCCATGC AGAACGCCGT 
CTTTGCCAAG AATGTGGCAA GATGTTTACT AAAGAGGCTT TCAGGGCTCA TGTCCGAGTT 
CACCAGGCTA AGAGAATGGG AGAGGTCCTC AAGCACATCT GTGAAATCTG TGGCAAAGCC 
TACCGCTTCA AGTGCAGCTT GAACTCTCAC ATGCGCTGCC ACACAGGAGT CCGGGACTTT 
GACTGTAAGA TATGTGGTAA GAAATTCCTC TCGGCCCATG GGCTGGAGAA ACATGAACTG 
GTCCATCGTA AAGTAAAGCC CCACGTGTGC CCTGATTGTG GGCGTGGCTT CACCCAGTTC 
CATAACATGC TTGCCCATCG CAGGCAGCAC ACCGGTGAGA AACCTTACCG CTGTAACTTC 
TGCGACCGTT CATTCACCCA TAATGTCAGC CTGAAGAATC ATAAGAAGAA AGAGCATGGG 
GTTGACCTAT GGCAAGGGAC GGGTGGGAAA CCCCCCTCCT CAAGTCGTTC ACGGTCCAAG 
TCGTCAAGAA GTGCCCAGGC TGTTGTTCCT GTCAGTCCTC CTGTACCCCA GCAGCCACCT 
GCACAGCCAA AGCCATCAGT GCCAAGTGAA AGAGAGTTAA TGCATACATT AGATCCTGCT 
CAGCTATATA ACATGATGCC CACTCTGGAT TATGGGCATT ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC579112 cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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