LOC578723 cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC578723
Entrez Gene ID	578723
Full Name	CUB and sushi domain-containing protein 2
Gene Type	protein-coding
Organism	Strongylocentrotus purpuratus(purple sea urchin)

mRNA and Protein(s)

mRNA	Protein	Name
XM_778878.4	XP_783971.3	CUB and sushi domain-containing protein 2
XM_778878.5	XP_783971.4	tolloid-like protein 1

Pathways from BioSystems
Gene Ontology

The following LOC578723 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC578723 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSd04782	XM_778878.4 Latest version!	Strongylocentrotus purpuratus CUB and sushi domain-containing protein 2 (LOC578723), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OSd56545	XM_778878.5 Latest version!	Strongylocentrotus purpuratus tolloid-like protein 1 (LOC578723), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC578723 ( XM_778878.4 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_783971.3 Strongylocentrotus purpuratus CUB and sushi domain-containing protein 2 (LOC578723), mRNA.

Price & Availability↑

CloneID	OSd04782
Clone ID Related Accession (Same CDS sequence)	XM_778878.4
Accession Version	XM_778878.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2613bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1427040000000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	CUB and sushi domain-containing protein 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011976913.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 24, 2015 this sequence version replaced XM_778878.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Strongylocentrotus purpuratus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGACTT TAATGGGGTT TTGTTTATTG GTGATCTGGT TGGGCTTGGT AGCCGCCAAA 
CCTAGACCAC TCTTTCTCAA CGATGGTGAG GAAAAAGTGG ATTGGATGGT TGAGGCAAAG 
TCGAGGACAC GGCGTCAGTC AGTGACGTCA TTCAAAGAGG CGGAGCAGAA ACTTAGGTTA 
CTCAGGGTTC GTATGAACGA TATCGACGAA GACCTCAGAC CATGCGACGA GGAGGATGTC 
TTGAAGGGTG TCCAGTGCAT TGAAGAAGAC AAGTGTATCA CATGGGACCA GGTATGTGAC 
GATGTCCAGG ACTGTGGTCT TCAGCAGCTC GGAGCATGCC TCAACCAGAC CGTGCAGCAG 
TACCGAGTGG AGCGGGAAGA CCTGTGCAAC GGAGACGAGA ACAGGCTATG GTGCGATCTC 
AAGGAACACA TTCCACGGAG ATGTGGTGAA GACATCGATC TGTCCGAGAG GACCAACAAG 
ACTGTTCGAA TTTTGTCGCC CAACTACCCC CGACAGTACC CGAGTGACAT CCACTGTTAC 
TGGATGATCA AGACCGCAGA GGGTAGCACT CTCAGCGCCT CATTCAAGAA GTTCGACACC 
GAGCCCGACT ACGACTTCTT CAGTTTAGGA ACAGGAGAGA AAGAAGATGA GGAGGACCCT 
ACTAAGTACC TGATCTTCAA GCACAGTGGT CCAGAGCTGC CAGACCCTAC ATCTTTCAAA 
ATCAAGGGAG ATACCATGTG GATTTCATTC AGGAGTGATG ACTGGGATAA CTATTCAGGC 
TTTGAGGTCC ACGTTGCTGA TACCTCTGTT TTTGATCCTG GTAATGTTGC CAAAAGTCCG 
TTCCCCGATG CCAGGAATAC AACTTGTGGT GGCACGGTCA GATTGGACAT TGATGGGGAG 
GAGACGATTA CGTCACCAGG GTATCCAGAG CAGTATGGTA ACTACATGCA ATGTGATTGG 
TTAGTCAGGA TCGTTCCGGG ACGACGTATC GGTATCTCCT TCAGGACCTT TGAGCTTGAG 
ACTAATCAGG ACTTTGTGGA GATCGGATCT GGTGCCAGCG TTGGAACAGA TCTTGTCAAT 
GGCGGGAAGC TCACTGGACA TTCAGTTCCT CACAAGGCCA TCGTGATCGA GAACAACGTG 
GCCTGGATAA GGTTCACGAG TGACCCGGCC AATGCCCGAC GTGGCTTCTC AGCAATAGTC 
AGGGACCAAC CTCACAATGG TTGTGGTGGA ACAATTGACA TCCCCAACGA CGGGTCTATC 
GTGGTCGCCT CTCCCCTCTT CCCCGACCTC GGTTACCGGG TCAACCTCAA CTGCATCTGG 
AAGTTCACTG GATCTGAGGG CCGCACGCTC TCAGTCATAT TCAAAGACTT CGCTACCGAG 
GAGAAATACG ACACCGTGAG CGCTGGCTAC GGGAGCAGCC CGGCCCGCGA TGGATCCAAC 
TATGTGATCA AGAGCGCCAG TGGTAGTGGA CTGCCTGAGC CAGCAGGGTT CGATACCCCT 
TCCAATGAAG CTTGGATCTA CTTCCGATCC GACAGTTCCA TCATCGATCG CGGATTCAAA 
GTAGAAGTAT CAGATACCTC GATCAGGCCT GCCAACTTTA CACCTGAGGT TCAACCTACC 
AAGGAGCCCG ACACAAATTG TGGTGGCGAG GTTGTGGGAG GCTCCTTCAC TACCAAGCTG 
GTGATTGACT CACCAGGTGG TTCTACGTAC AGGAAGTTCC TGGACTGTTT GTGGGAGATC 
GAAATTCCTT TCGGTGGCCA AATCCGCCTC TTTTTCCCGC AGTTCCAAAC CGAGCAATAC 
CGCGACATCG TCATCGTCGG CAACGGAACG GACACCGAAG ACAACGAGAG CATTTTCATC 
ACCCACAGCG GCCGAAAGAA GCCTAAGGAA GTCCTAACAT ACGCTAACAA CCTGTGGGTC 
CGGTTCACCA CAGATTCCGA CTATGAATTG TCCGGTTGGA CCATGGTCGT GGAAGCAATA 
CCCTATACAA ATTGCAGTGG TCACCTCTCC ATCCCACCCG AAGGCTCCAT CACAATCGGT 
TCGCCAAACT TCCCCTACAA CTACAACAAT GGGGAGACGT GTTACTATCA GATCATCGGT 
GCCCCTGGTA AAAGGATACT GGTCAATTTT CTGGAGTTTA ACACGGAACG AGATTTTCGA 
GACGTTATGA ACATCGGAGA CGGATGCTTC CTGGCGAGCG GGGATATCAT TGTCAGAAAC 
CACAGTGGCC ATGGCTTACC AGATCCTGGG AGATACTTCT CCCGCCAAAA CGCAGTGTGG 
ATGACTTTCC AAACCAGTGA TTTCAGCGAT TACAAGGGAT GGCTCATCAA CTTTGCCGAC 
GCCAAAACCC CCGCTGATGC CGGCGCTGAA TGCGAGGTGT TCGTGCCCCC AACCCAACCT 
CCCGCAACCA CCAAAGAGCC ATACCAGCCT ATAATCTACG GTGCGGATGA AATCTGGTCC 
GGCCTTGGTC CCATCCCCGC CTGGTACGAT GATCTCAACC CCGACGCAGA CGGCGCAGGG 
GATGGGGACA CAAATACCAT GACAGACTTC GAGTACTCCT CTCCTGTGGA GGAACGCGTC 
GTTGAGGGAA GTGTCCCTGC AGATAGCGCG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_783971.3
CDS	575..3187
Translation	MRTLMGFCLLVIWLGLVAAKPRPLFLNDGEEKVDWMVEAKSRTRRQSVTSFKEAEQKLRLLRVRMNDIDEDLRPCDEEDVLKGVQCIEEDKCITWDQVCDDVQDCGLQQLGACLNQTVQQYRVEREDLCNGDENRLWCDLKEHIPRRCGEDIDLSERTNKTVRILSPNYPRQYPSDIHCYWMIKTAEGSTLSASFKKFDTEPDYDFFSLGTGEKEDEEDPTKYLIFKHSGPELPDPTSFKIKGDTMWISFRSDDWDNYSGFEVHVADTSVFDPGNVAKSPFPDARNTTCGGTVRLDIDGEETITSPGYPEQYGNYMQCDWLVRIVPGRRIGISFRTFELETNQDFVEIGSGASVGTDLVNGGKLTGHSVPHKAIVIENNVAWIRFTSDPANARRGFSAIVRDQPHNGCGGTIDIPNDGSIVVASPLFPDLGYRVNLNCIWKFTGSEGRTLSVIFKDFATEEKYDTVSAGYGSSPARDGSNYVIKSASGSGLPEPAGFDTPSNEAWIYFRSDSSIIDRGFKVEVSDTSIRPANFTPEVQPTKEPDTNCGGEVVGGSFTTKLVIDSPGGSTYRKFLDCLWEIEIPFGGQIRLFFPQFQTEQYRDIVIVGNGTDTEDNESIFITHSGRKKPKEVLTYANNLWVRFTTDSDYELSGWTMVVEAIPYTNCSGHLSIPPEGSITIGSPNFPYNYNNGETCYYQIIGAPGKRILVNFLEFNTERDFRDVMNIGDGCFLASGDIIVRNHSGHGLPDPGRYFSRQNAVWMTFQTSDFSDYKGWLINFADAKTPADAGAECEVFVPPTQPPATTKEPYQPIIYGADEIWSGLGPIPAWYDDLNPDADGAGDGDTNTMTDFEYSSPVEERVVEGSVPADSA

Target ORF information:

RefSeq Version	XM_778878.4
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus CUB and sushi domain-containing protein 2 (LOC578723), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_778878.4

ORF Insert Sequence:

ATGAGGACTT TAATGGGGTT TTGTTTATTG GTGATCTGGT TGGGCTTGGT AGCCGCCAAA 
CCTAGACCAC TCTTTCTCAA CGATGGTGAG GAAAAAGTGG ATTGGATGGT TGAGGCAAAG 
TCGAGGACAC GGCGTCAGTC AGTGACGTCA TTCAAAGAGG CGGAGCAGAA ACTTAGGTTA 
CTCAGGGTTC GTATGAACGA TATCGACGAA GACCTCAGAC CATGCGACGA GGAGGATGTC 
TTGAAGGGTG TCCAGTGCAT TGAAGAAGAC AAGTGTATCA CATGGGACCA GGTATGTGAC 
GATGTCCAGG ACTGTGGTCT TCAGCAGCTC GGAGCATGCC TCAACCAGAC CGTGCAGCAG 
TACCGAGTGG AGCGGGAAGA CCTGTGCAAC GGAGACGAGA ACAGGCTATG GTGCGATCTC 
AAGGAACACA TTCCACGGAG ATGTGGTGAA GACATCGATC TGTCCGAGAG GACCAACAAG 
ACTGTTCGAA TTTTGTCGCC CAACTACCCC CGACAGTACC CGAGTGACAT CCACTGTTAC 
TGGATGATCA AGACCGCAGA GGGTAGCACT CTCAGCGCCT CATTCAAGAA GTTCGACACC 
GAGCCCGACT ACGACTTCTT CAGTTTAGGA ACAGGAGAGA AAGAAGATGA GGAGGACCCT 
ACTAAGTACC TGATCTTCAA GCACAGTGGT CCAGAGCTGC CAGACCCTAC ATCTTTCAAA 
ATCAAGGGAG ATACCATGTG GATTTCATTC AGGAGTGATG ACTGGGATAA CTATTCAGGC 
TTTGAGGTCC ACGTTGCTGA TACCTCTGTT TTTGATCCTG GTAATGTTGC CAAAAGTCCG 
TTCCCCGATG CCAGGAATAC AACTTGTGGT GGCACGGTCA GATTGGACAT TGATGGGGAG 
GAGACGATTA CGTCACCAGG GTATCCAGAG CAGTATGGTA ACTACATGCA ATGTGATTGG 
TTAGTCAGGA TCGTTCCGGG ACGACGTATC GGTATCTCCT TCAGGACCTT TGAGCTTGAG 
ACTAATCAGG ACTTTGTGGA GATCGGATCT GGTGCCAGCG TTGGAACAGA TCTTGTCAAT 
GGCGGGAAGC TCACTGGACA TTCAGTTCCT CACAAGGCCA TCGTGATCGA GAACAACGTG 
GCCTGGATAA GGTTCACGAG TGACCCGGCC AATGCCCGAC GTGGCTTCTC AGCAATAGTC 
AGGGACCAAC CTCACAATGG TTGTGGTGGA ACAATTGACA TCCCCAACGA CGGGTCTATC 
GTGGTCGCCT CTCCCCTCTT CCCCGACCTC GGTTACCGGG TCAACCTCAA CTGCATCTGG 
AAGTTCACTG GATCTGAGGG CCGCACGCTC TCAGTCATAT TCAAAGACTT CGCTACCGAG 
GAGAAATACG ACACCGTGAG CGCTGGCTAC GGGAGCAGCC CGGCCCGCGA TGGATCCAAC 
TATGTGATCA AGAGCGCCAG TGGTAGTGGA CTGCCTGAGC CAGCAGGGTT CGATACCCCT 
TCCAATGAAG CTTGGATCTA CTTCCGATCC GACAGTTCCA TCATCGATCG CGGATTCAAA 
GTAGAAGTAT CAGATACCTC GATCAGGCCT GCCAACTTTA CACCTGAGGT TCAACCTACC 
AAGGAGCCCG ACACAAATTG TGGTGGCGAG GTTGTGGGAG GCTCCTTCAC TACCAAGCTG 
GTGATTGACT CACCAGGTGG TTCTACGTAC AGGAAGTTCC TGGACTGTTT GTGGGAGATC 
GAAATTCCTT TCGGTGGCCA AATCCGCCTC TTTTTCCCGC AGTTCCAAAC CGAGCAATAC 
CGCGACATCG TCATCGTCGG CAACGGAACG GACACCGAAG ACAACGAGAG CATTTTCATC 
ACCCACAGCG GCCGAAAGAA GCCTAAGGAA GTCCTAACAT ACGCTAACAA CCTGTGGGTC 
CGGTTCACCA CAGATTCCGA CTATGAATTG TCCGGTTGGA CCATGGTCGT GGAAGCAATA 
CCCTATACAA ATTGCAGTGG TCACCTCTCC ATCCCACCCG AAGGCTCCAT CACAATCGGT 
TCGCCAAACT TCCCCTACAA CTACAACAAT GGGGAGACGT GTTACTATCA GATCATCGGT 
GCCCCTGGTA AAAGGATACT GGTCAATTTT CTGGAGTTTA ACACGGAACG AGATTTTCGA 
GACGTTATGA ACATCGGAGA CGGATGCTTC CTGGCGAGCG GGGATATCAT TGTCAGAAAC 
CACAGTGGCC ATGGCTTACC AGATCCTGGG AGATACTTCT CCCGCCAAAA CGCAGTGTGG 
ATGACTTTCC AAACCAGTGA TTTCAGCGAT TACAAGGGAT GGCTCATCAA CTTTGCCGAC 
GCCAAAACCC CCGCTGATGC CGGCGCTGAA TGCGAGGTGT TCGTGCCCCC AACCCAACCT 
CCCGCAACCA CCAAAGAGCC ATACCAGCCT ATAATCTACG GTGCGGATGA AATCTGGTCC 
GGCCTTGGTC CCATCCCCGC CTGGTACGAT GATCTCAACC CCGACGCAGA CGGCGCAGGG 
GATGGGGACA CAAATACCAT GACAGACTTC GAGTACTCCT CTCCTGTGGA GGAACGCGTC 
GTTGAGGGAA GTGTCCCTGC AGATAGCGCG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC578723 ( XM_778878.5 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_783971.4 Strongylocentrotus purpuratus tolloid-like protein 1 (LOC578723), mRNA.

Price & Availability↑

CloneID	OSd56545
Clone ID Related Accession (Same CDS sequence)	XM_778878.5
Accession Version	XM_778878.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2613bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569427200000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	tolloid-like protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_022145604.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 27, 2019 this sequence version replaced XM_778878.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Strongylocentrotus purpuratus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGACTT TAATGGGGTT TTGTTTATTG GTGATCTGGT TGGGCTTGGT AGCCGCCAAA 
CCTAGACCAC TCTTTCTCAA CGATGGTGAG GAAAAAGTGG ATTGGATGGT TGAGGCAAAG 
TCGAGGACAC GGCGTCAGTC AGTGACGTCA TTCAAAGAGG CGGAGCAGAA ACTTAGGTTA 
CTCAGGGTTC GTATGAACGA TATCGACGAA GACCTCAGAC CATGCGACGA GGAGGATGTC 
TTGAAGGGTG TCCAGTGCAT TGAAGAAGAC AAGTGTATCA CATGGGACCA GGTATGTGAC 
GATGTCCAGG ACTGTGGTCT TCAGCAGCTC GGAGCATGCC TCAACCAGAC CGTGCAGCAG 
TACCGAGTGG AGCGGGAAGA CCTGTGCAAC GGAGACGAGA ACAGGCTATG GTGCGATCTC 
AAGGAACACA TTCCACGGAG ATGTGGTGAA GACATCGATC TGTCCGAGAG GACCAACAAG 
ACTGTTCGAA TTTTGTCGCC CAACTACCCC CGACAGTACC CGAGTGACAT CCACTGTTAC 
TGGATGATCA AGACCGCAGA GGGTAGCACT CTCAGCGCCT CATTCAAGAA GTTCGACACC 
GAGCCCGACT ACGACTTCTT CAGTTTAGGA ACAGGAGAGA AAGAAGATGA GGAGGACCCT 
ACTAAGTACC TGATCTTCAA GCACAGTGGT CCAGAGCTGC CAGACCCTAC ATCTTTCAAA 
ATCAAGGGAG ATACCATGTG GATTTCATTC AGGAGTGATG ACTGGGATAA CTATTCAGGC 
TTTGAGGTCC ACGTTGCTGA TACCTCTGTT TTCGATCCTG GTAATGTTGC CAAAAGTCCG 
TTCCCCGATG CCAGGAATAC AACTTGTGGT GGCACGGTCA GATTGGACAT TGATGGGGAG 
GAGACGATTA CGTCACCAGG GTATCCAGAG CAGTATGGTA ACTACATGCA ATGTGATTGG 
TTAGTCAGGA TCGTTCCCGG ACGACGTATC GGTATCTCCT TCAGGACCTT TGAGCTTGAG 
ACTAATCAGG ACTTTGTGGA GATCGGATCT GGTGCCAGCG TTGGAACAGA TCTTGTCAAT 
GGCGGGAAAC TCACTGGACA TTCAGTTCCT CACAAGGCCA TTGTGATCGA GAACAACGTG 
GCCTGGATAA GGTTCACCAG TGATCCGGCC AATGCCCGAC GTGGCTTCTC AGCAATAGTC 
AGGGACCAAC CTCATAATGG TTGTGGTGGA ACAATTGACA TCCCCAACGA CGGGTCTATC 
GTGGTCGCCT CTCCCCTCTT CCCCGACCTC GGTTACCGGG TCAACCTCAA CTGCATCTGG 
AAGTTCACTG GATCTGAGGG CCGCACGCTC TCAGTCATAT TCAAAGACTT CGCTACCGAG 
GAGAAATACG ACACCGTGAG CGCTGGCTAC GGGAGCAGCC CGGCCCGCGA TGGATCCAAC 
TATGTGATCA AGAGCGCCAG TGGTAGTGGA CTGCCTGAGC CAGCAGGGTT CGATACCCCT 
TCCAATGAAG CTTGGATCTA CTTCCGATCC GACAGCTCCA TCATCGATCG CGGATTCAAA 
GTAGAAGTAT CAGATACCTC GATCAGGCCT GCCAACTTTA CACCTGAGGT TCAACCTACC 
AAGGAGCCAG ACACAAATTG TGGTGGCGAG GTTGTGGGAG GCTCCTTCAC TACCAAGCTG 
GTGATTGACT CACCAGGTGG TTCTACGTAC AGGAAGTTCC TGGACTGTTT GTGGGAGATC 
GAAATTCCTT TCGGTGGCCA AATCCGCCTC TTTTTCCCGC AGTTCCAAAC CGAGCAATAC 
CGCGACATCG TCATCGTCGG CAACGGAACG GACTATGAAG ACAACGAGAG CATTTTCATC 
ACCCACAGCG GCCGAAAGAA GCCTAAGGAA GTCCTAACAT ACGCTAACAA CCTGTGGGTC 
CGGTTCACCA CAGATTCCGA CTATGAATTG TCCGGTTGGA CCATGGTCGT GGAAGCAATA 
CCCTATACAA ATTGCAGTGG TCACCTCTCC ATCCCACCCG AAGGCTCCAT CACAATCGGT 
TCGCCAAACT TCCCCTACAA CTACAACAAT GGGGAGACGT GTTACTATCA GATCATCGGT 
GCCCCTGGTA AAAGGATACT GGTCAATTTT CTGGAGTTTA ACACGGAACG AGATTTTCGA 
GACGTTATGA ACATCGGAGA CGGATGCTTC CTGGCGAGCG GGGATATCAT TGTCAGAAAC 
CATAGTGGCC ATGGCTTACC TGATCCTGGG AGATACTTCT CCCGTCAAAA CGCAGTGTGG 
ATGACTTTCC AAACCAGTGA TTTCAGCGAT TACAAGGGAT GGCTCATCAA CTTTGCCGAC 
GCCAAAACCC CCGCTGATGC CGGCGCTGAA TGCGAGGTGT TCGTGCCCCC AACCCAACCT 
CCCGCAACCA CCAAAGAGCC ATACCAGCCT ATAATCTACG GTGCGGATGA AATCTGGTCC 
GGCCTTGGTC CCATCCCCGC CTGGTACGAT GATCTCAACC CCGACGCAGA CGGCGCAGGG 
GATGGGGACA CAAATACCAT GACAGACTTC GAGTACTCCT CTCCTGTGGA GGAACGCGTC 
GTTGAGGGAA GTGTCCCTGC AGATAGCGCG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_783971.4
CDS	630..3242
Translation	MRTLMGFCLLVIWLGLVAAKPRPLFLNDGEEKVDWMVEAKSRTRRQSVTSFKEAEQKLRLLRVRMNDIDEDLRPCDEEDVLKGVQCIEEDKCITWDQVCDDVQDCGLQQLGACLNQTVQQYRVEREDLCNGDENRLWCDLKEHIPRRCGEDIDLSERTNKTVRILSPNYPRQYPSDIHCYWMIKTAEGSTLSASFKKFDTEPDYDFFSLGTGEKEDEEDPTKYLIFKHSGPELPDPTSFKIKGDTMWISFRSDDWDNYSGFEVHVADTSVFDPGNVAKSPFPDARNTTCGGTVRLDIDGEETITSPGYPEQYGNYMQCDWLVRIVPGRRIGISFRTFELETNQDFVEIGSGASVGTDLVNGGKLTGHSVPHKAIVIENNVAWIRFTSDPANARRGFSAIVRDQPHNGCGGTIDIPNDGSIVVASPLFPDLGYRVNLNCIWKFTGSEGRTLSVIFKDFATEEKYDTVSAGYGSSPARDGSNYVIKSASGSGLPEPAGFDTPSNEAWIYFRSDSSIIDRGFKVEVSDTSIRPANFTPEVQPTKEPDTNCGGEVVGGSFTTKLVIDSPGGSTYRKFLDCLWEIEIPFGGQIRLFFPQFQTEQYRDIVIVGNGTDYEDNESIFITHSGRKKPKEVLTYANNLWVRFTTDSDYELSGWTMVVEAIPYTNCSGHLSIPPEGSITIGSPNFPYNYNNGETCYYQIIGAPGKRILVNFLEFNTERDFRDVMNIGDGCFLASGDIIVRNHSGHGLPDPGRYFSRQNAVWMTFQTSDFSDYKGWLINFADAKTPADAGAECEVFVPPTQPPATTKEPYQPIIYGADEIWSGLGPIPAWYDDLNPDADGAGDGDTNTMTDFEYSSPVEERVVEGSVPADSA

Target ORF information:

RefSeq Version	XM_778878.5
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus tolloid-like protein 1 (LOC578723), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_778878.5

ORF Insert Sequence:

ATGAGGACTT TAATGGGGTT TTGTTTATTG GTGATCTGGT TGGGCTTGGT AGCCGCCAAA 
CCTAGACCAC TCTTTCTCAA CGATGGTGAG GAAAAAGTGG ATTGGATGGT TGAGGCAAAG 
TCGAGGACAC GGCGTCAGTC AGTGACGTCA TTCAAAGAGG CGGAGCAGAA ACTTAGGTTA 
CTCAGGGTTC GTATGAACGA TATCGACGAA GACCTCAGAC CATGCGACGA GGAGGATGTC 
TTGAAGGGTG TCCAGTGCAT TGAAGAAGAC AAGTGTATCA CATGGGACCA GGTATGTGAC 
GATGTCCAGG ACTGTGGTCT TCAGCAGCTC GGAGCATGCC TCAACCAGAC CGTGCAGCAG 
TACCGAGTGG AGCGGGAAGA CCTGTGCAAC GGAGACGAGA ACAGGCTATG GTGCGATCTC 
AAGGAACACA TTCCACGGAG ATGTGGTGAA GACATCGATC TGTCCGAGAG GACCAACAAG 
ACTGTTCGAA TTTTGTCGCC CAACTACCCC CGACAGTACC CGAGTGACAT CCACTGTTAC 
TGGATGATCA AGACCGCAGA GGGTAGCACT CTCAGCGCCT CATTCAAGAA GTTCGACACC 
GAGCCCGACT ACGACTTCTT CAGTTTAGGA ACAGGAGAGA AAGAAGATGA GGAGGACCCT 
ACTAAGTACC TGATCTTCAA GCACAGTGGT CCAGAGCTGC CAGACCCTAC ATCTTTCAAA 
ATCAAGGGAG ATACCATGTG GATTTCATTC AGGAGTGATG ACTGGGATAA CTATTCAGGC 
TTTGAGGTCC ACGTTGCTGA TACCTCTGTT TTCGATCCTG GTAATGTTGC CAAAAGTCCG 
TTCCCCGATG CCAGGAATAC AACTTGTGGT GGCACGGTCA GATTGGACAT TGATGGGGAG 
GAGACGATTA CGTCACCAGG GTATCCAGAG CAGTATGGTA ACTACATGCA ATGTGATTGG 
TTAGTCAGGA TCGTTCCCGG ACGACGTATC GGTATCTCCT TCAGGACCTT TGAGCTTGAG 
ACTAATCAGG ACTTTGTGGA GATCGGATCT GGTGCCAGCG TTGGAACAGA TCTTGTCAAT 
GGCGGGAAAC TCACTGGACA TTCAGTTCCT CACAAGGCCA TTGTGATCGA GAACAACGTG 
GCCTGGATAA GGTTCACCAG TGATCCGGCC AATGCCCGAC GTGGCTTCTC AGCAATAGTC 
AGGGACCAAC CTCATAATGG TTGTGGTGGA ACAATTGACA TCCCCAACGA CGGGTCTATC 
GTGGTCGCCT CTCCCCTCTT CCCCGACCTC GGTTACCGGG TCAACCTCAA CTGCATCTGG 
AAGTTCACTG GATCTGAGGG CCGCACGCTC TCAGTCATAT TCAAAGACTT CGCTACCGAG 
GAGAAATACG ACACCGTGAG CGCTGGCTAC GGGAGCAGCC CGGCCCGCGA TGGATCCAAC 
TATGTGATCA AGAGCGCCAG TGGTAGTGGA CTGCCTGAGC CAGCAGGGTT CGATACCCCT 
TCCAATGAAG CTTGGATCTA CTTCCGATCC GACAGCTCCA TCATCGATCG CGGATTCAAA 
GTAGAAGTAT CAGATACCTC GATCAGGCCT GCCAACTTTA CACCTGAGGT TCAACCTACC 
AAGGAGCCAG ACACAAATTG TGGTGGCGAG GTTGTGGGAG GCTCCTTCAC TACCAAGCTG 
GTGATTGACT CACCAGGTGG TTCTACGTAC AGGAAGTTCC TGGACTGTTT GTGGGAGATC 
GAAATTCCTT TCGGTGGCCA AATCCGCCTC TTTTTCCCGC AGTTCCAAAC CGAGCAATAC 
CGCGACATCG TCATCGTCGG CAACGGAACG GACTATGAAG ACAACGAGAG CATTTTCATC 
ACCCACAGCG GCCGAAAGAA GCCTAAGGAA GTCCTAACAT ACGCTAACAA CCTGTGGGTC 
CGGTTCACCA CAGATTCCGA CTATGAATTG TCCGGTTGGA CCATGGTCGT GGAAGCAATA 
CCCTATACAA ATTGCAGTGG TCACCTCTCC ATCCCACCCG AAGGCTCCAT CACAATCGGT 
TCGCCAAACT TCCCCTACAA CTACAACAAT GGGGAGACGT GTTACTATCA GATCATCGGT 
GCCCCTGGTA AAAGGATACT GGTCAATTTT CTGGAGTTTA ACACGGAACG AGATTTTCGA 
GACGTTATGA ACATCGGAGA CGGATGCTTC CTGGCGAGCG GGGATATCAT TGTCAGAAAC 
CATAGTGGCC ATGGCTTACC TGATCCTGGG AGATACTTCT CCCGTCAAAA CGCAGTGTGG 
ATGACTTTCC AAACCAGTGA TTTCAGCGAT TACAAGGGAT GGCTCATCAA CTTTGCCGAC 
GCCAAAACCC CCGCTGATGC CGGCGCTGAA TGCGAGGTGT TCGTGCCCCC AACCCAACCT 
CCCGCAACCA CCAAAGAGCC ATACCAGCCT ATAATCTACG GTGCGGATGA AATCTGGTCC 
GGCCTTGGTC CCATCCCCGC CTGGTACGAT GATCTCAACC CCGACGCAGA CGGCGCAGGG 
GATGGGGACA CAAATACCAT GACAGACTTC GAGTACTCCT CTCCTGTGGA GGAACGCGTC 
GTTGAGGGAA GTGTCCCTGC AGATAGCGCG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC578723 cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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