LOC577182 cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC577182
Entrez Gene ID	577182
Full Name	amoeboid myosin I
Gene Type	protein-coding
Organism	Strongylocentrotus purpuratus(purple sea urchin)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001124762.1	NP_001118234.1	amoeboid myosin I
XM_030976264.1	XP_030832124.1	amoeboid myosin I isoform X1
XM_011685575.1	XP_011683877.1	amoeboid myosin I isoform X1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene structure in the sea urchin Strongylocentrotus purpuratus based on transcriptome analysis.
Tu Q, Cameron RA, Worley KC, Gibbs RA, Davidson EH
Genome research22(10)2079-87(2012 Oct)

Characterization of sea urchin unconventional myosins and analysis of their patterns of expression during early embryogenesis.
Sirotkin V, Seipel S, Krendel M, Bonder EM
Molecular reproduction and development57(2)111-26(2000 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC577182 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC577182 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSd20318	NM_001124762.1 Latest version!	Strongylocentrotus purpuratus amoeboid myosin I (LOC577182), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OSd20318	XM_011685575.1 Latest version!	Strongylocentrotus purpuratus amoeboid myosin I (LOC577182), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OSd71219	XM_030976264.1 Latest version!	Strongylocentrotus purpuratus amoeboid myosin I (LOC577182), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC577182 ( NM_001124762.1 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> NP_001118234.1 Strongylocentrotus purpuratus amoeboid myosin I (LOC577182), mRNA.

Price & Availability↑

CloneID	OSd20318
Clone ID Related Accession (Same CDS sequence)	NM_001124762.1 , XM_011685575.1
Accession Version	NM_001124762.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3357bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556899200000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	amoeboid myosin I
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAGJ05128884.1, AAGJ05128879.1, AAGJ05128878.1 and AAGJ05128877.1. On Apr 23, 2008 this sequence version replaced XM_777426.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: JT097042.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN01096960, SAMN01103178 [ECO:0000350] ##Evidence-Data-END##

ATGGCCTACC ACTGGCAGAG TCAGGGTCGC AAGACCAGTG GAGTGGAGGA CATGGTTCTA 
CTCAGCAAGA TTCAAGAGGG CGCTATAGTG GAGAACCTGA AGAAACGCTT CATGGATGAC 
TTGATCTATA CATACATCGG CCCTGTGCTG ATATCCATCA ATCCATTCAA ACAGATGCCA 
TACTTCACAG AGAAGGAGGT TGATATGTAC CAAGGAGCTG CCATATATGA GAACCCCCCT 
CATGTGTACG CCCTTACAGA TGACATGTAC AGAAACATGT TGATAGAATA CGAGAACCAA 
TGCGTAATCA TCAGTGGAGA GAGTGGAGCT GGAAAGACCG TCGCAGCCAA GTATATCATG 
GCGTACATCG CCAAGGTTTC CGGGGGAGGA GCTACTGTTC AGCACGTCAA AGAAGTCATT 
ATCCAATCAA ATCCGCTCTT AGAAGCCTTC GGTAACGCCA AGACAGTGAG GAACAACAAC 
TCGAGTAGAT TTGGGAAGTA TGTAGAGATC CAGTTCACCA GAGCAGGAGC ACCTGATGGA 
GGGAAGATAA CAAACTTCCT GCTGGAGAAG TCCAGAGTGG TCAGTCAAAA CACTGATGAA 
AGGAACTTTC ATATCTTCTA TCAGCTCCTG TGTGGGGCCA ACCATCAACA AAAAGAGAAT 
CTTGGTCTGA CAACACCAGA CTACTACTTC TACCTGAACC AGAGTGGATC ATACAAAGTG 
GATGGGTTAG ACGATGTCAA AGAATACAAG GATACTCTGA ATGCCATGTC TGTGATTGGA 
ATATCCGAAG ATGATCAAGA GAATGTTCTC TCCTGCGTGG CTGGCATACT CCATCTTGGA 
AACATTGTGT TTGTAGAAAA GGACAACTAT GCTGTCATAC ATGATGATGA GTTTCTTGAT 
TTCCCGTCCT ACCTGCTTGG GCTGGACAAG GAAGCGCTAC GTAAGAAGCT GATCAGCCGT 
CGTATGGACA GTAAATGGGG CGGGAAGGCG GAGCATATAG AGGTCACACT TAACACAGAG 
CAAGCAGCTC ATACCAGAGA TGCTCTAGCT AAGGCTGTCC ATTCAAGACT ATTTGACTTC 
CTCGTTAGTT CCATCAATGG TGCCATGCAG AAGCAGAAGG TAGAGATCAC AATAGGAGTT 
CTGGATATCT ATGGCTTTGA GATCTTTCAA CAGAATGGCT TTGAACAGTT CTGCATTAAC 
TTTGTGAACG AGAAGCTTCA GCAGATCTTT ATAGAACTCA CACTCAAAGC TGAGCAGGAA 
GAGTATGTAG CAGAAGGGAT CAAGTGGAAG AAGATTGATT ACTTCAACAA CAAGATTGTG 
TGTGATCTCA TTGAGAGCAA GGTACCTCCC GGAGTCATGT GCATCCTGGA TGACGTGTGT 
GCGACCATGC ATGCCGTGAG CGATGGTGCC GATGAAAAGC TTCTACAGAA GATGAACAGT 
GCCGTGGGAA CGCATCAGCA CTACGGGGTC TTGAGTGGGG GATTCATTGT ACATCATTAC 
GCTGGCCAGG TGCGGTATAC AGTAGAAGGA TTCTGTGAGA GGAATCGTGA TGTACTCTTC 
ACTGATCTCA TCGAACTTAT GCAGTCAAGT GAGAACTCGT TCTTCATGAC CCTATTTCCC 
GAGAAGATCG CGGGTCAAGC CAAGTCACGA CCCACTACAG CTAGCAGCAA AATCAAAACT 
CAGGCCAACA AGCTAGTAGA CAAACTGATG CAATGTACAC CTCATTACAT CCGCTGTATC 
AAACCAAACG AGACAAAGAG ACCTCAGGAC TGGGAAGAAA AAAGGGTATA TCATCAAGTT 
GAATACCTGG GACTGAAGGA GAACATCAGG GTGAGGCGAG CTGGTTTCGC TTACAGGAGA 
GAATTTGACA AATTTCTCAG ACGGTACGCC ATCTTGACTC CCGAGACATG GCCAAGGTGG 
GGAGGTGATG TCAAGTCTGG GGTCAATCAT CTACTACGCG CTGTCAACAT GGACACTGAT 
GAGTTTCAGC TAGGCAAGAC AAAGGTCTTC ATCAGGAAAC CAGAATCTCT CTTTCTCCTA 
GAAGAACAGA GAGAGAGAAA GTTTGATTAT CATGCTCGGA TCATCCAGAA GGCATTCAGG 
CAATTCAATG CACAGAAGTA CTATCTCAGA CTCAAACAGC AAGCCAGTGA CATCCTGTTC 
GACAAGAAGG AGAGGAGGCG CTACAGTCTC AACCGTAACT TTGTCGGGGA CTACATCGGC 
TTTGAGCACA ATGTATCCCT GCAGGCGTTG GTTGCTAGGA GAGAGAGGAT TGAGTTTGCT 
GATAAGGTCA ACAAGTATGA CAGGAGATTC AAGGTCACGA AGCGAGACCT GCTACTATCA 
GGCAGGAACA TCTATCTGAT TGGTCGAGAG CAGATCAAGA AAGGGCCAGA GAAAGGTCAG 
ATCAGGGACG TCGTCAAAAG GAAGATCCCC CTCAACCATA TACACAGTGT ATCTCTCAGT 
ACTCTTCAGG ATGACATGTT TGTGCTCCAT GTAAGTGAGG AATATGACAG CATCTTAGAG 
TCCACTTTCA AGACTGAATT CCTGACCGTA CTCAACAAGA AGTTCCAAGA GACTCTACAA 
CAGAAACTGA TGGTCAACTT CAATGACTCG ATCGAGTTTT CAGTGAAGAA GGAAGGATGG 
GGTGGAGGAG GAACGAGGAC AGTGAGGTTT GCTCAGGGTC AGGGAGACAC TGCCGTTCTC 
AAGCCGTCCA GCAAGATACT GACCGTTAGC ATTGGTAGTG GACTTCCACG CAACTCAAGA 
CCTGGACTAC GTGATGTCAA GGTGACGAGC AAAGACAGGC AGGCCGGTAA CCGCGGCAAC 
AGTAACCGTG GCAACAGTAA TCGCGGCAAT GGACATGTAT CAAGGAGGCC CGCTCCCAGC 
CAGCCTGCCC CAGGCAAACC CACTGCTCCG TATGCCCTGG TACAGAAGAG AGGGGGTCCT 
ACCAGCCCCA CCAAAGGAGG CATGAAACGT ACTTCAAGTC GAAGGAAGAG TGGAGAGCAG 
CCCCATCTAC CAAAAGGGGG CGCCCAGATC TTCGCCAAAC AGTTCAAGAA CGTGATCAAC 
AGCCAAGACT ACCTACAGAC ACCAGACGCT GGGGTATCAG GGCAAAAACG TAGGACCATA 
AAGGGCAGCC GCCCTGCACC TGGAGCTGGT AGACCCAAGC CCAAACCACA ACCACCAGCC 
CCGTCCAGAC CAAAGTGTCG TACCATCTAC CCCTACGATG CGGGCGATAC GGATGAGTTG 
TCATTCGGAG AAGGAGAGAC TATAGAAATC ATTTTGGAAG ATGCCTCTGG TTGGTGGAGA 
GGTAAGCTAC GAGGGAGAGA GGGACTCTTC CCGGCCAATT ACATTGAAAA GATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001118234.1
CDS	215..3571
Translation	MAYHWQSQGRKTSGVEDMVLLSKIQEGAIVENLKKRFMDDLIYTYIGPVLISINPFKQMPYFTEKEVDMYQGAAIYENPPHVYALTDDMYRNMLIEYENQCVIISGESGAGKTVAAKYIMAYIAKVSGGGATVQHVKEVIIQSNPLLEAFGNAKTVRNNNSSRFGKYVEIQFTRAGAPDGGKITNFLLEKSRVVSQNTDERNFHIFYQLLCGANHQQKENLGLTTPDYYFYLNQSGSYKVDGLDDVKEYKDTLNAMSVIGISEDDQENVLSCVAGILHLGNIVFVEKDNYAVIHDDEFLDFPSYLLGLDKEALRKKLISRRMDSKWGGKAEHIEVTLNTEQAAHTRDALAKAVHSRLFDFLVSSINGAMQKQKVEITIGVLDIYGFEIFQQNGFEQFCINFVNEKLQQIFIELTLKAEQEEYVAEGIKWKKIDYFNNKIVCDLIESKVPPGVMCILDDVCATMHAVSDGADEKLLQKMNSAVGTHQHYGVLSGGFIVHHYAGQVRYTVEGFCERNRDVLFTDLIELMQSSENSFFMTLFPEKIAGQAKSRPTTASSKIKTQANKLVDKLMQCTPHYIRCIKPNETKRPQDWEEKRVYHQVEYLGLKENIRVRRAGFAYRREFDKFLRRYAILTPETWPRWGGDVKSGVNHLLRAVNMDTDEFQLGKTKVFIRKPESLFLLEEQRERKFDYHARIIQKAFRQFNAQKYYLRLKQQASDILFDKKERRRYSLNRNFVGDYIGFEHNVSLQALVARRERIEFADKVNKYDRRFKVTKRDLLLSGRNIYLIGREQIKKGPEKGQIRDVVKRKIPLNHIHSVSLSTLQDDMFVLHVSEEYDSILESTFKTEFLTVLNKKFQETLQQKLMVNFNDSIEFSVKKEGWGGGGTRTVRFAQGQGDTAVLKPSSKILTVSIGSGLPRNSRPGLRDVKVTSKDRQAGNRGNSNRGNSNRGNGHVSRRPAPSQPAPGKPTAPYALVQKRGGPTSPTKGGMKRTSSRRKSGEQPHLPKGGAQIFAKQFKNVINSQDYLQTPDAGVSGQKRRTIKGSRPAPGAGRPKPKPQPPAPSRPKCRTIYPYDAGDTDELSFGEGETIEIILEDASGWWRGKLRGREGLFPANYIEKI

Target ORF information:

RefSeq Version	NM_001124762.1
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus amoeboid myosin I (LOC577182), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001124762.1

ORF Insert Sequence:

ATGGCCTACC ACTGGCAGAG TCAGGGTCGC AAGACCAGTG GAGTGGAGGA CATGGTTCTA 
CTCAGCAAGA TTCAAGAGGG CGCTATAGTG GAGAACCTGA AGAAACGCTT CATGGATGAC 
TTGATCTATA CATACATCGG CCCTGTGCTG ATATCCATCA ATCCATTCAA ACAGATGCCA 
TACTTCACAG AGAAGGAGGT TGATATGTAC CAAGGAGCTG CCATATATGA GAACCCCCCT 
CATGTGTACG CCCTTACAGA TGACATGTAC AGAAACATGT TGATAGAATA CGAGAACCAA 
TGCGTAATCA TCAGTGGAGA GAGTGGAGCT GGAAAGACCG TCGCAGCCAA GTATATCATG 
GCGTACATCG CCAAGGTTTC CGGGGGAGGA GCTACTGTTC AGCACGTCAA AGAAGTCATT 
ATCCAATCAA ATCCGCTCTT AGAAGCCTTC GGTAACGCCA AGACAGTGAG GAACAACAAC 
TCGAGTAGAT TTGGGAAGTA TGTAGAGATC CAGTTCACCA GAGCAGGAGC ACCTGATGGA 
GGGAAGATAA CAAACTTCCT GCTGGAGAAG TCCAGAGTGG TCAGTCAAAA CACTGATGAA 
AGGAACTTTC ATATCTTCTA TCAGCTCCTG TGTGGGGCCA ACCATCAACA AAAAGAGAAT 
CTTGGTCTGA CAACACCAGA CTACTACTTC TACCTGAACC AGAGTGGATC ATACAAAGTG 
GATGGGTTAG ACGATGTCAA AGAATACAAG GATACTCTGA ATGCCATGTC TGTGATTGGA 
ATATCCGAAG ATGATCAAGA GAATGTTCTC TCCTGCGTGG CTGGCATACT CCATCTTGGA 
AACATTGTGT TTGTAGAAAA GGACAACTAT GCTGTCATAC ATGATGATGA GTTTCTTGAT 
TTCCCGTCCT ACCTGCTTGG GCTGGACAAG GAAGCGCTAC GTAAGAAGCT GATCAGCCGT 
CGTATGGACA GTAAATGGGG CGGGAAGGCG GAGCATATAG AGGTCACACT TAACACAGAG 
CAAGCAGCTC ATACCAGAGA TGCTCTAGCT AAGGCTGTCC ATTCAAGACT ATTTGACTTC 
CTCGTTAGTT CCATCAATGG TGCCATGCAG AAGCAGAAGG TAGAGATCAC AATAGGAGTT 
CTGGATATCT ATGGCTTTGA GATCTTTCAA CAGAATGGCT TTGAACAGTT CTGCATTAAC 
TTTGTGAACG AGAAGCTTCA GCAGATCTTT ATAGAACTCA CACTCAAAGC TGAGCAGGAA 
GAGTATGTAG CAGAAGGGAT CAAGTGGAAG AAGATTGATT ACTTCAACAA CAAGATTGTG 
TGTGATCTCA TTGAGAGCAA GGTACCTCCC GGAGTCATGT GCATCCTGGA TGACGTGTGT 
GCGACCATGC ATGCCGTGAG CGATGGTGCC GATGAAAAGC TTCTACAGAA GATGAACAGT 
GCCGTGGGAA CGCATCAGCA CTACGGGGTC TTGAGTGGGG GATTCATTGT ACATCATTAC 
GCTGGCCAGG TGCGGTATAC AGTAGAAGGA TTCTGTGAGA GGAATCGTGA TGTACTCTTC 
ACTGATCTCA TCGAACTTAT GCAGTCAAGT GAGAACTCGT TCTTCATGAC CCTATTTCCC 
GAGAAGATCG CGGGTCAAGC CAAGTCACGA CCCACTACAG CTAGCAGCAA AATCAAAACT 
CAGGCCAACA AGCTAGTAGA CAAACTGATG CAATGTACAC CTCATTACAT CCGCTGTATC 
AAACCAAACG AGACAAAGAG ACCTCAGGAC TGGGAAGAAA AAAGGGTATA TCATCAAGTT 
GAATACCTGG GACTGAAGGA GAACATCAGG GTGAGGCGAG CTGGTTTCGC TTACAGGAGA 
GAATTTGACA AATTTCTCAG ACGGTACGCC ATCTTGACTC CCGAGACATG GCCAAGGTGG 
GGAGGTGATG TCAAGTCTGG GGTCAATCAT CTACTACGCG CTGTCAACAT GGACACTGAT 
GAGTTTCAGC TAGGCAAGAC AAAGGTCTTC ATCAGGAAAC CAGAATCTCT CTTTCTCCTA 
GAAGAACAGA GAGAGAGAAA GTTTGATTAT CATGCTCGGA TCATCCAGAA GGCATTCAGG 
CAATTCAATG CACAGAAGTA CTATCTCAGA CTCAAACAGC AAGCCAGTGA CATCCTGTTC 
GACAAGAAGG AGAGGAGGCG CTACAGTCTC AACCGTAACT TTGTCGGGGA CTACATCGGC 
TTTGAGCACA ATGTATCCCT GCAGGCGTTG GTTGCTAGGA GAGAGAGGAT TGAGTTTGCT 
GATAAGGTCA ACAAGTATGA CAGGAGATTC AAGGTCACGA AGCGAGACCT GCTACTATCA 
GGCAGGAACA TCTATCTGAT TGGTCGAGAG CAGATCAAGA AAGGGCCAGA GAAAGGTCAG 
ATCAGGGACG TCGTCAAAAG GAAGATCCCC CTCAACCATA TACACAGTGT ATCTCTCAGT 
ACTCTTCAGG ATGACATGTT TGTGCTCCAT GTAAGTGAGG AATATGACAG CATCTTAGAG 
TCCACTTTCA AGACTGAATT CCTGACCGTA CTCAACAAGA AGTTCCAAGA GACTCTACAA 
CAGAAACTGA TGGTCAACTT CAATGACTCG ATCGAGTTTT CAGTGAAGAA GGAAGGATGG 
GGTGGAGGAG GAACGAGGAC AGTGAGGTTT GCTCAGGGTC AGGGAGACAC TGCCGTTCTC 
AAGCCGTCCA GCAAGATACT GACCGTTAGC ATTGGTAGTG GACTTCCACG CAACTCAAGA 
CCTGGACTAC GTGATGTCAA GGTGACGAGC AAAGACAGGC AGGCCGGTAA CCGCGGCAAC 
AGTAACCGTG GCAACAGTAA TCGCGGCAAT GGACATGTAT CAAGGAGGCC CGCTCCCAGC 
CAGCCTGCCC CAGGCAAACC CACTGCTCCG TATGCCCTGG TACAGAAGAG AGGGGGTCCT 
ACCAGCCCCA CCAAAGGAGG CATGAAACGT ACTTCAAGTC GAAGGAAGAG TGGAGAGCAG 
CCCCATCTAC CAAAAGGGGG CGCCCAGATC TTCGCCAAAC AGTTCAAGAA CGTGATCAAC 
AGCCAAGACT ACCTACAGAC ACCAGACGCT GGGGTATCAG GGCAAAAACG TAGGACCATA 
AAGGGCAGCC GCCCTGCACC TGGAGCTGGT AGACCCAAGC CCAAACCACA ACCACCAGCC 
CCGTCCAGAC CAAAGTGTCG TACCATCTAC CCCTACGATG CGGGCGATAC GGATGAGTTG 
TCATTCGGAG AAGGAGAGAC TATAGAAATC ATTTTGGAAG ATGCCTCTGG TTGGTGGAGA 
GGTAAGCTAC GAGGGAGAGA GGGACTCTTC CCGGCCAATT ACATTGAAAA GATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC577182 ( XM_011685575.1 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_011683877.1 Strongylocentrotus purpuratus amoeboid myosin I (LOC577182), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSd20318
Clone ID Related Accession (Same CDS sequence)	NM_001124762.1 , XM_011685575.1
Accession Version	XM_011685575.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3357bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1427040000000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	amoeboid myosin I isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011996200.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Strongylocentrotus purpuratus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTACC ACTGGCAGAG TCAGGGTCGC AAGACCAGTG GAGTGGAGGA CATGGTTCTA 
CTCAGCAAGA TTCAAGAGGG CGCTATAGTG GAGAACCTGA AGAAACGCTT CATGGATGAC 
TTGATCTATA CATACATCGG CCCTGTGCTG ATATCCATCA ATCCATTCAA ACAGATGCCA 
TACTTCACAG AGAAGGAGGT TGATATGTAC CAAGGAGCTG CCATATATGA GAACCCCCCT 
CATGTGTACG CCCTTACAGA TGACATGTAC AGAAACATGT TGATAGAATA CGAGAACCAA 
TGCGTAATCA TCAGTGGAGA GAGTGGAGCT GGAAAGACCG TCGCAGCCAA GTATATCATG 
GCGTACATCG CCAAGGTTTC CGGGGGAGGA GCTACTGTTC AGCACGTCAA AGAAGTCATT 
ATCCAATCAA ATCCGCTCTT AGAAGCCTTC GGTAACGCCA AGACAGTGAG GAACAACAAC 
TCGAGTAGAT TTGGGAAGTA TGTAGAGATC CAGTTCACCA GAGCAGGAGC ACCTGATGGA 
GGGAAGATAA CAAACTTCCT GCTGGAGAAG TCCAGAGTGG TCAGTCAAAA CACTGATGAA 
AGGAACTTTC ATATCTTCTA TCAGCTCCTG TGTGGGGCCA ACCATCAACA AAAAGAGAAT 
CTTGGTCTGA CAACACCAGA CTACTACTTC TACCTGAACC AGAGTGGATC ATACAAAGTG 
GATGGGTTAG ACGATGTCAA AGAATACAAG GATACTCTGA ATGCCATGTC TGTGATTGGA 
ATATCCGAAG ATGATCAAGA GAATGTTCTC TCCTGCGTGG CTGGCATACT CCATCTTGGA 
AACATTGTGT TTGTAGAAAA GGACAACTAT GCTGTCATAC ATGATGATGA GTTTCTTGAT 
TTCCCGTCCT ACCTGCTTGG GCTGGACAAG GAAGCGCTAC GTAAGAAGCT GATCAGCCGT 
CGTATGGACA GTAAATGGGG CGGGAAGGCG GAGCATATAG AGGTCACACT TAACACAGAG 
CAAGCAGCTC ATACCAGAGA TGCTCTAGCT AAGGCTGTCC ATTCAAGACT ATTTGACTTC 
CTCGTTAGTT CCATCAATGG TGCCATGCAG AAGCAGAAGG TAGAGATCAC AATAGGAGTT 
CTGGATATCT ATGGCTTTGA GATCTTTCAA CAGAATGGCT TTGAACAGTT CTGCATTAAC 
TTTGTGAACG AGAAGCTTCA GCAGATCTTT ATAGAACTCA CACTCAAAGC TGAGCAGGAA 
GAGTATGTAG CAGAAGGGAT CAAGTGGAAG AAGATTGATT ACTTCAACAA CAAGATTGTG 
TGTGATCTCA TTGAGAGCAA GGTACCTCCC GGAGTCATGT GCATCCTGGA TGACGTGTGT 
GCGACCATGC ATGCCGTGAG CGATGGTGCC GATGAAAAGC TTCTACAGAA GATGAACAGT 
GCCGTGGGAA CGCATCAGCA CTACGGGGTC TTGAGTGGGG GATTCATTGT ACATCATTAC 
GCTGGCCAGG TGCGGTATAC AGTAGAAGGA TTCTGTGAGA GGAATCGTGA TGTACTCTTC 
ACTGATCTCA TCGAACTTAT GCAGTCAAGT GAGAACTCGT TCTTCATGAC CCTATTTCCC 
GAGAAGATCG CGGGTCAAGC CAAGTCACGA CCCACTACAG CTAGCAGCAA AATCAAAACT 
CAGGCCAACA AGCTAGTAGA CAAACTGATG CAATGTACAC CTCATTACAT CCGCTGTATC 
AAACCAAACG AGACAAAGAG ACCTCAGGAC TGGGAAGAAA AAAGGGTATA TCATCAAGTT 
GAATACCTGG GACTGAAGGA GAACATCAGG GTGAGGCGAG CTGGTTTCGC TTACAGGAGA 
GAATTTGACA AATTTCTCAG ACGGTACGCC ATCTTGACTC CCGAGACATG GCCAAGGTGG 
GGAGGTGATG TCAAGTCTGG GGTCAATCAT CTACTACGCG CTGTCAACAT GGACACTGAT 
GAGTTTCAGC TAGGCAAGAC AAAGGTCTTC ATCAGGAAAC CAGAATCTCT CTTTCTCCTA 
GAAGAACAGA GAGAGAGAAA GTTTGATTAT CATGCTCGGA TCATCCAGAA GGCATTCAGG 
CAATTCAATG CACAGAAGTA CTATCTCAGA CTCAAACAGC AAGCCAGTGA CATCCTGTTC 
GACAAGAAGG AGAGGAGGCG CTACAGTCTC AACCGTAACT TTGTCGGGGA CTACATCGGC 
TTTGAGCACA ATGTATCCCT GCAGGCGTTG GTTGCTAGGA GAGAGAGGAT TGAGTTTGCT 
GATAAGGTCA ACAAGTATGA CAGGAGATTC AAGGTCACGA AGCGAGACCT GCTACTATCA 
GGCAGGAACA TCTATCTGAT TGGTCGAGAG CAGATCAAGA AAGGGCCAGA GAAAGGTCAG 
ATCAGGGACG TCGTCAAAAG GAAGATCCCC CTCAACCATA TACACAGTGT ATCTCTCAGT 
ACTCTTCAGG ATGACATGTT TGTGCTCCAT GTAAGTGAGG AATATGACAG CATCTTAGAG 
TCCACTTTCA AGACTGAATT CCTGACCGTA CTCAACAAGA AGTTCCAAGA GACTCTACAA 
CAGAAACTGA TGGTCAACTT CAATGACTCG ATCGAGTTTT CAGTGAAGAA GGAAGGATGG 
GGTGGAGGAG GAACGAGGAC AGTGAGGTTT GCTCAGGGTC AGGGAGACAC TGCCGTTCTC 
AAGCCGTCCA GCAAGATACT GACCGTTAGC ATTGGTAGTG GACTTCCACG CAACTCAAGA 
CCTGGACTAC GTGATGTCAA GGTGACGAGC AAAGACAGGC AGGCCGGTAA CCGCGGCAAC 
AGTAACCGTG GCAACAGTAA TCGCGGCAAT GGACATGTAT CAAGGAGGCC CGCTCCCAGC 
CAGCCTGCCC CAGGCAAACC CACTGCTCCG TATGCCCTGG TACAGAAGAG AGGGGGTCCT 
ACCAGCCCCA CCAAAGGAGG CATGAAACGT ACTTCAAGTC GAAGGAAGAG TGGAGAGCAG 
CCCCATCTAC CAAAAGGGGG CGCCCAGATC TTCGCCAAAC AGTTCAAGAA CGTGATCAAC 
AGCCAAGACT ACCTACAGAC ACCAGACGCT GGGGTATCAG GGCAAAAACG TAGGACCATA 
AAGGGCAGCC GCCCTGCACC TGGAGCTGGT AGACCCAAGC CCAAACCACA ACCACCAGCC 
CCGTCCAGAC CAAAGTGTCG TACCATCTAC CCCTACGATG CGGGCGATAC GGATGAGTTG 
TCATTCGGAG AAGGAGAGAC TATAGAAATC ATTTTGGAAG ATGCCTCTGG TTGGTGGAGA 
GGTAAGCTAC GAGGGAGAGA GGGACTCTTC CCGGCCAATT ACATTGAAAA GATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011683877.1
CDS	533..3889
Translation	MAYHWQSQGRKTSGVEDMVLLSKIQEGAIVENLKKRFMDDLIYTYIGPVLISINPFKQMPYFTEKEVDMYQGAAIYENPPHVYALTDDMYRNMLIEYENQCVIISGESGAGKTVAAKYIMAYIAKVSGGGATVQHVKEVIIQSNPLLEAFGNAKTVRNNNSSRFGKYVEIQFTRAGAPDGGKITNFLLEKSRVVSQNTDERNFHIFYQLLCGANHQQKENLGLTTPDYYFYLNQSGSYKVDGLDDVKEYKDTLNAMSVIGISEDDQENVLSCVAGILHLGNIVFVEKDNYAVIHDDEFLDFPSYLLGLDKEALRKKLISRRMDSKWGGKAEHIEVTLNTEQAAHTRDALAKAVHSRLFDFLVSSINGAMQKQKVEITIGVLDIYGFEIFQQNGFEQFCINFVNEKLQQIFIELTLKAEQEEYVAEGIKWKKIDYFNNKIVCDLIESKVPPGVMCILDDVCATMHAVSDGADEKLLQKMNSAVGTHQHYGVLSGGFIVHHYAGQVRYTVEGFCERNRDVLFTDLIELMQSSENSFFMTLFPEKIAGQAKSRPTTASSKIKTQANKLVDKLMQCTPHYIRCIKPNETKRPQDWEEKRVYHQVEYLGLKENIRVRRAGFAYRREFDKFLRRYAILTPETWPRWGGDVKSGVNHLLRAVNMDTDEFQLGKTKVFIRKPESLFLLEEQRERKFDYHARIIQKAFRQFNAQKYYLRLKQQASDILFDKKERRRYSLNRNFVGDYIGFEHNVSLQALVARRERIEFADKVNKYDRRFKVTKRDLLLSGRNIYLIGREQIKKGPEKGQIRDVVKRKIPLNHIHSVSLSTLQDDMFVLHVSEEYDSILESTFKTEFLTVLNKKFQETLQQKLMVNFNDSIEFSVKKEGWGGGGTRTVRFAQGQGDTAVLKPSSKILTVSIGSGLPRNSRPGLRDVKVTSKDRQAGNRGNSNRGNSNRGNGHVSRRPAPSQPAPGKPTAPYALVQKRGGPTSPTKGGMKRTSSRRKSGEQPHLPKGGAQIFAKQFKNVINSQDYLQTPDAGVSGQKRRTIKGSRPAPGAGRPKPKPQPPAPSRPKCRTIYPYDAGDTDELSFGEGETIEIILEDASGWWRGKLRGREGLFPANYIEKI

Target ORF information:

RefSeq Version	XM_011685575.1
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus amoeboid myosin I (LOC577182), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011685575.1

ORF Insert Sequence:

ATGGCCTACC ACTGGCAGAG TCAGGGTCGC AAGACCAGTG GAGTGGAGGA CATGGTTCTA 
CTCAGCAAGA TTCAAGAGGG CGCTATAGTG GAGAACCTGA AGAAACGCTT CATGGATGAC 
TTGATCTATA CATACATCGG CCCTGTGCTG ATATCCATCA ATCCATTCAA ACAGATGCCA 
TACTTCACAG AGAAGGAGGT TGATATGTAC CAAGGAGCTG CCATATATGA GAACCCCCCT 
CATGTGTACG CCCTTACAGA TGACATGTAC AGAAACATGT TGATAGAATA CGAGAACCAA 
TGCGTAATCA TCAGTGGAGA GAGTGGAGCT GGAAAGACCG TCGCAGCCAA GTATATCATG 
GCGTACATCG CCAAGGTTTC CGGGGGAGGA GCTACTGTTC AGCACGTCAA AGAAGTCATT 
ATCCAATCAA ATCCGCTCTT AGAAGCCTTC GGTAACGCCA AGACAGTGAG GAACAACAAC 
TCGAGTAGAT TTGGGAAGTA TGTAGAGATC CAGTTCACCA GAGCAGGAGC ACCTGATGGA 
GGGAAGATAA CAAACTTCCT GCTGGAGAAG TCCAGAGTGG TCAGTCAAAA CACTGATGAA 
AGGAACTTTC ATATCTTCTA TCAGCTCCTG TGTGGGGCCA ACCATCAACA AAAAGAGAAT 
CTTGGTCTGA CAACACCAGA CTACTACTTC TACCTGAACC AGAGTGGATC ATACAAAGTG 
GATGGGTTAG ACGATGTCAA AGAATACAAG GATACTCTGA ATGCCATGTC TGTGATTGGA 
ATATCCGAAG ATGATCAAGA GAATGTTCTC TCCTGCGTGG CTGGCATACT CCATCTTGGA 
AACATTGTGT TTGTAGAAAA GGACAACTAT GCTGTCATAC ATGATGATGA GTTTCTTGAT 
TTCCCGTCCT ACCTGCTTGG GCTGGACAAG GAAGCGCTAC GTAAGAAGCT GATCAGCCGT 
CGTATGGACA GTAAATGGGG CGGGAAGGCG GAGCATATAG AGGTCACACT TAACACAGAG 
CAAGCAGCTC ATACCAGAGA TGCTCTAGCT AAGGCTGTCC ATTCAAGACT ATTTGACTTC 
CTCGTTAGTT CCATCAATGG TGCCATGCAG AAGCAGAAGG TAGAGATCAC AATAGGAGTT 
CTGGATATCT ATGGCTTTGA GATCTTTCAA CAGAATGGCT TTGAACAGTT CTGCATTAAC 
TTTGTGAACG AGAAGCTTCA GCAGATCTTT ATAGAACTCA CACTCAAAGC TGAGCAGGAA 
GAGTATGTAG CAGAAGGGAT CAAGTGGAAG AAGATTGATT ACTTCAACAA CAAGATTGTG 
TGTGATCTCA TTGAGAGCAA GGTACCTCCC GGAGTCATGT GCATCCTGGA TGACGTGTGT 
GCGACCATGC ATGCCGTGAG CGATGGTGCC GATGAAAAGC TTCTACAGAA GATGAACAGT 
GCCGTGGGAA CGCATCAGCA CTACGGGGTC TTGAGTGGGG GATTCATTGT ACATCATTAC 
GCTGGCCAGG TGCGGTATAC AGTAGAAGGA TTCTGTGAGA GGAATCGTGA TGTACTCTTC 
ACTGATCTCA TCGAACTTAT GCAGTCAAGT GAGAACTCGT TCTTCATGAC CCTATTTCCC 
GAGAAGATCG CGGGTCAAGC CAAGTCACGA CCCACTACAG CTAGCAGCAA AATCAAAACT 
CAGGCCAACA AGCTAGTAGA CAAACTGATG CAATGTACAC CTCATTACAT CCGCTGTATC 
AAACCAAACG AGACAAAGAG ACCTCAGGAC TGGGAAGAAA AAAGGGTATA TCATCAAGTT 
GAATACCTGG GACTGAAGGA GAACATCAGG GTGAGGCGAG CTGGTTTCGC TTACAGGAGA 
GAATTTGACA AATTTCTCAG ACGGTACGCC ATCTTGACTC CCGAGACATG GCCAAGGTGG 
GGAGGTGATG TCAAGTCTGG GGTCAATCAT CTACTACGCG CTGTCAACAT GGACACTGAT 
GAGTTTCAGC TAGGCAAGAC AAAGGTCTTC ATCAGGAAAC CAGAATCTCT CTTTCTCCTA 
GAAGAACAGA GAGAGAGAAA GTTTGATTAT CATGCTCGGA TCATCCAGAA GGCATTCAGG 
CAATTCAATG CACAGAAGTA CTATCTCAGA CTCAAACAGC AAGCCAGTGA CATCCTGTTC 
GACAAGAAGG AGAGGAGGCG CTACAGTCTC AACCGTAACT TTGTCGGGGA CTACATCGGC 
TTTGAGCACA ATGTATCCCT GCAGGCGTTG GTTGCTAGGA GAGAGAGGAT TGAGTTTGCT 
GATAAGGTCA ACAAGTATGA CAGGAGATTC AAGGTCACGA AGCGAGACCT GCTACTATCA 
GGCAGGAACA TCTATCTGAT TGGTCGAGAG CAGATCAAGA AAGGGCCAGA GAAAGGTCAG 
ATCAGGGACG TCGTCAAAAG GAAGATCCCC CTCAACCATA TACACAGTGT ATCTCTCAGT 
ACTCTTCAGG ATGACATGTT TGTGCTCCAT GTAAGTGAGG AATATGACAG CATCTTAGAG 
TCCACTTTCA AGACTGAATT CCTGACCGTA CTCAACAAGA AGTTCCAAGA GACTCTACAA 
CAGAAACTGA TGGTCAACTT CAATGACTCG ATCGAGTTTT CAGTGAAGAA GGAAGGATGG 
GGTGGAGGAG GAACGAGGAC AGTGAGGTTT GCTCAGGGTC AGGGAGACAC TGCCGTTCTC 
AAGCCGTCCA GCAAGATACT GACCGTTAGC ATTGGTAGTG GACTTCCACG CAACTCAAGA 
CCTGGACTAC GTGATGTCAA GGTGACGAGC AAAGACAGGC AGGCCGGTAA CCGCGGCAAC 
AGTAACCGTG GCAACAGTAA TCGCGGCAAT GGACATGTAT CAAGGAGGCC CGCTCCCAGC 
CAGCCTGCCC CAGGCAAACC CACTGCTCCG TATGCCCTGG TACAGAAGAG AGGGGGTCCT 
ACCAGCCCCA CCAAAGGAGG CATGAAACGT ACTTCAAGTC GAAGGAAGAG TGGAGAGCAG 
CCCCATCTAC CAAAAGGGGG CGCCCAGATC TTCGCCAAAC AGTTCAAGAA CGTGATCAAC 
AGCCAAGACT ACCTACAGAC ACCAGACGCT GGGGTATCAG GGCAAAAACG TAGGACCATA 
AAGGGCAGCC GCCCTGCACC TGGAGCTGGT AGACCCAAGC CCAAACCACA ACCACCAGCC 
CCGTCCAGAC CAAAGTGTCG TACCATCTAC CCCTACGATG CGGGCGATAC GGATGAGTTG 
TCATTCGGAG AAGGAGAGAC TATAGAAATC ATTTTGGAAG ATGCCTCTGG TTGGTGGAGA 
GGTAAGCTAC GAGGGAGAGA GGGACTCTTC CCGGCCAATT ACATTGAAAA GATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC577182 ( XM_030976264.1 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_030832124.1 Strongylocentrotus purpuratus amoeboid myosin I (LOC577182), transcript variant X1, mRNA.

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CloneID	OSd71219
Clone ID Related Accession (Same CDS sequence)	XM_030976264.1
Accession Version	XM_030976264.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3357bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569427200000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	amoeboid myosin I isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_022145615.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Strongylocentrotus purpuratus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTACC ACTGGCAGAG TCAGGGTCGC AAGACCAGTG GAGTGGAGGA CATGGTTCTA 
CTCAGCAAGA TTCAAGAGGG CGCTATAGTG GAGAACCTGA AGAAACGCTT CATGGATGAC 
TTGATCTATA CATACATCGG CCCTGTGCTG ATATCCATCA ATCCATTCAA ACAGATGCCA 
TACTTCACAG AGAAGGAGGT TGATATGTAC CAAGGAGCTG CCATATATGA GAACCCCCCT 
CATGTGTACG CCCTTACAGA TGACATGTAC AGAAACATGT TGATAGAATA CGAGAACCAA 
TGCGTAATCA TCAGTGGAGA GAGTGGAGCT GGAAAGACCG TCGCAGCCAA GTATATCATG 
GCGTACATCG CCAAGGTTTC CGGGGGAGGA GCTACTGTTC AGCACGTCAA AGAAGTCATT 
ATCCAATCAA ATCCGCTCTT AGAAGCCTTC GGTAACGCCA AGACAGTGAG GAACAACAAC 
TCGAGTAGAT TTGGGAAGTA TGTAGAGATC CAGTTCACCA GAGCAGGAGC ACCTGATGGA 
GGGAAGATAA CAAACTTCCT GCTGGAGAAG TCCAGAGTGG TCAGTCAAAA CACTGATGAA 
AGGAACTTTC ATATCTTCTA TCAGCTCCTG TGTGGGGCCA ACCATCAACA AAAAGAGAAT 
CTTGGTCTGA CAACACCAGA CTACTACTTC TACCTGAACC AGAGTGGATC ATACAAAGTG 
GATGGGTTAG ACGATGTCAA AGAATACAAG GATACTCTGA ATGCCATGTC TGTGATTGGA 
ATATCCGAAG ATGATCAAGA GAATGTTCTC TCCTGCGTGG CTGGCATACT CCATCTTGGA 
AACATTGTGT TTGTAGAAAA GGACAACTAT GCTGTCATAC ATGATGATGA GTTTCTTGAT 
TTCCCGTCCT ACCTGCTTGG GCTGGACAAG GAAGCGCTAC GTAAGAAGCT GATCAGCCGT 
CGTATGGACA GTAAATGGGG CGGGAAGGCG GAGCATATAG AGGTCACACT TAACACAGAG 
CAAGCAGCTC ATACCAGAGA TGCTCTAGCT AAGGCTGTCC ATTCAAGACT ATTTGACTTC 
CTCGTTAGTT CCATCAATGG TGCCATGCAG AAGCAGAAGG TAGAGATCAC AATAGGAGTT 
CTGGATATCT ATGGCTTTGA GATCTTTCAA CAGAATGGCT TTGAACAGTT CTGCATTAAC 
TTTGTGAACG AGAAGCTTCA GCAGATCTTT ATAGAACTCA CACTCAAAGC TGAGCAGGAA 
GAGTATGTAG CAGAAGGGAT CAAGTGGAAG AAGATTGATT ACTTCAACAA CAAGATTGTG 
TGTGATCTCA TTGAGAGCAA GGTACCTCCC GGAGTCATGT GCATCCTGGA TGACGTGTGT 
GCGACCATGC ATGCCGTGAG CGATGGTGCC GATGAAAAGC TTCTACAGAA GATGAACAGT 
GCCGTGGGAA CGCATCAGCA CTACGGGGTC TTGAGTGGGG GATTCATTGT ACATCATTAC 
GCTGGCCAGG TGCGGTATAC AGTAGAAGGA TTCTGTGAGA GGAATCGTGA TGTACTCTTC 
ACTGATCTCA TCGAACTTAT GCAGTCAAGT GAGAACTCGT TCTTCATGAC CCTATTTCCC 
GAGAAGATCG CGGGTCAAGC CAAGTCACGA CCCACTACAG CTAGCAGCAA AATCAAAACT 
CAGGCCAACA AGCTAGTAGA CAAACTGATG CAATGTACAC CTCATTACAT CCGCTGTATC 
AAACCAAACG AGACAAAGAG ACCTCAGGAC TGGGAAGAAA AAAGGGTATA TCATCAAGTT 
GAATACCTGG GACTGAAGGA GAACATCAGG GTGAGGCGAG CTGGTTTCGC TTACAGGAGA 
GAATTCGACA AATTTCTCAG ACGGTATGCT ATCTTGACTC CCGAGACATG GCCAAGGTGG 
GGAGGTGATG TCAAGTCTGG GGTCAATCAT CTACTACGCG CTGTCAACAT GGACACTGAT 
GAGTTTCAGC TAGGCAAGAC AAAGGTCTTC ATCAGGAAAC CAGAATCTCT CTTTCTCCTA 
GAAGAACAGA GAGAGAGAAA GTTTGACTTT CATGCTCGGA TCATCCAGAA GGCATTCAGA 
CAATTCAATG CACAGAAGTA CTATCTCAGA CTCAAACAGC AAGCCAGTGA CATCCTGTTC 
GACAAGAAGG AGAGGAGGCG CTACAGTCTC AACCGTAACT TTGTCGGGGA CTACATCGGC 
TTTGAGCACA ATGTATCCCT GCAGGCGTTG GTTGCTAGGA GAGAGAGGAT TGAGTTTGCT 
GATAAGGTCA ACAAGTATGA CAGGAGATTC AAGGTCACGA AGCGAGACCT GCTACTATCA 
GGCAGGAATA TCTATCTGAT TGGTCGAGAG CAGATCAAGA AAGGGCCAGA GAAAGGTCAG 
ATCAGGGACG TGGTCAAAAG GAAGATTCCC CTCAACCATA TACACAGTGT ATCTCTCAGT 
ACTCTTCAGG ATGACATGTT TGTGCTCCAT GTAAGTGAGG AATATGACAG CATCTTAGAG 
TCCACTTTCA AGACTGAATT CCTGACCGTA CTCAACAAGA AGTTCCAAGA GACTCTACAA 
CAGAAACTGA TGGTCAACTT CAATGACTCG ATCGAGTTTT CAGTGAAGAA GGAAGGATGG 
GGTGGAGGAG GAACGAGGAC AGTGAGGTTT GCTCAGGGTC AGGGAGACAC TGCCGTTCTC 
AAGCCGTCCA GCAAGATACT GACCGTTAGC ATTGGTAGTG GACTTCCACG CAACTCAAGA 
CCTGGACTAC GTGATGTCAA GGTGACGAGC AAAGACAGGC AGGCCGGTAA CCGCGGCAAC 
AGTAACCGTG GCAACAGTAA TCGCGGCAAT GGACATGTAT CAAGGAGGCC CGCTCCCAGC 
CAGCCTGCCC CAGGCAAACC CACTGCTCCG TATGCCCTGG TACAGAAGAG AGGGGGTCCT 
ACCAGCCCCA CCAAAGGAGG CATGAAACGT ACTTCAAGTC GAAGGAAGAG TGGAGAGCAG 
CCCCATCTAC CAAAAGGGGG CGCCCAGATC TTCGCCAAAC AGTTCAAGAA CGTGATCAAC 
AGCCAAGACT ACCTACAGAC ACCAGACGCT GGGGTATCAG GGCAAAAACG TAGGACCATA 
AAGGGCAGCC GCCCTGCACC TGGAGCTGGT AGACCCAAGC CCAAACCACA ACCACCAGCC 
CCGTCCAGAC CAAAGTGTCG TACCATCTAC CCCTACGATG CGGGCGATAC GGATGAGTTG 
TCATTCGGAG AAGGAGAGAC TATAGAAATC ATTTTGGAAG ATGCCTCTGG TTGGTGGAGA 
GGTAAGCTAC GAGGGAGAGA GGGACTCTTC CCGGCCAATT ACATTGAAAA GATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030832124.1
CDS	292..3648
Translation	MAYHWQSQGRKTSGVEDMVLLSKIQEGAIVENLKKRFMDDLIYTYIGPVLISINPFKQMPYFTEKEVDMYQGAAIYENPPHVYALTDDMYRNMLIEYENQCVIISGESGAGKTVAAKYIMAYIAKVSGGGATVQHVKEVIIQSNPLLEAFGNAKTVRNNNSSRFGKYVEIQFTRAGAPDGGKITNFLLEKSRVVSQNTDERNFHIFYQLLCGANHQQKENLGLTTPDYYFYLNQSGSYKVDGLDDVKEYKDTLNAMSVIGISEDDQENVLSCVAGILHLGNIVFVEKDNYAVIHDDEFLDFPSYLLGLDKEALRKKLISRRMDSKWGGKAEHIEVTLNTEQAAHTRDALAKAVHSRLFDFLVSSINGAMQKQKVEITIGVLDIYGFEIFQQNGFEQFCINFVNEKLQQIFIELTLKAEQEEYVAEGIKWKKIDYFNNKIVCDLIESKVPPGVMCILDDVCATMHAVSDGADEKLLQKMNSAVGTHQHYGVLSGGFIVHHYAGQVRYTVEGFCERNRDVLFTDLIELMQSSENSFFMTLFPEKIAGQAKSRPTTASSKIKTQANKLVDKLMQCTPHYIRCIKPNETKRPQDWEEKRVYHQVEYLGLKENIRVRRAGFAYRREFDKFLRRYAILTPETWPRWGGDVKSGVNHLLRAVNMDTDEFQLGKTKVFIRKPESLFLLEEQRERKFDFHARIIQKAFRQFNAQKYYLRLKQQASDILFDKKERRRYSLNRNFVGDYIGFEHNVSLQALVARRERIEFADKVNKYDRRFKVTKRDLLLSGRNIYLIGREQIKKGPEKGQIRDVVKRKIPLNHIHSVSLSTLQDDMFVLHVSEEYDSILESTFKTEFLTVLNKKFQETLQQKLMVNFNDSIEFSVKKEGWGGGGTRTVRFAQGQGDTAVLKPSSKILTVSIGSGLPRNSRPGLRDVKVTSKDRQAGNRGNSNRGNSNRGNGHVSRRPAPSQPAPGKPTAPYALVQKRGGPTSPTKGGMKRTSSRRKSGEQPHLPKGGAQIFAKQFKNVINSQDYLQTPDAGVSGQKRRTIKGSRPAPGAGRPKPKPQPPAPSRPKCRTIYPYDAGDTDELSFGEGETIEIILEDASGWWRGKLRGREGLFPANYIEKI

Target ORF information:

RefSeq Version	XM_030976264.1
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus amoeboid myosin I (LOC577182), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030976264.1

ORF Insert Sequence:

ATGGCCTACC ACTGGCAGAG TCAGGGTCGC AAGACCAGTG GAGTGGAGGA CATGGTTCTA 
CTCAGCAAGA TTCAAGAGGG CGCTATAGTG GAGAACCTGA AGAAACGCTT CATGGATGAC 
TTGATCTATA CATACATCGG CCCTGTGCTG ATATCCATCA ATCCATTCAA ACAGATGCCA 
TACTTCACAG AGAAGGAGGT TGATATGTAC CAAGGAGCTG CCATATATGA GAACCCCCCT 
CATGTGTACG CCCTTACAGA TGACATGTAC AGAAACATGT TGATAGAATA CGAGAACCAA 
TGCGTAATCA TCAGTGGAGA GAGTGGAGCT GGAAAGACCG TCGCAGCCAA GTATATCATG 
GCGTACATCG CCAAGGTTTC CGGGGGAGGA GCTACTGTTC AGCACGTCAA AGAAGTCATT 
ATCCAATCAA ATCCGCTCTT AGAAGCCTTC GGTAACGCCA AGACAGTGAG GAACAACAAC 
TCGAGTAGAT TTGGGAAGTA TGTAGAGATC CAGTTCACCA GAGCAGGAGC ACCTGATGGA 
GGGAAGATAA CAAACTTCCT GCTGGAGAAG TCCAGAGTGG TCAGTCAAAA CACTGATGAA 
AGGAACTTTC ATATCTTCTA TCAGCTCCTG TGTGGGGCCA ACCATCAACA AAAAGAGAAT 
CTTGGTCTGA CAACACCAGA CTACTACTTC TACCTGAACC AGAGTGGATC ATACAAAGTG 
GATGGGTTAG ACGATGTCAA AGAATACAAG GATACTCTGA ATGCCATGTC TGTGATTGGA 
ATATCCGAAG ATGATCAAGA GAATGTTCTC TCCTGCGTGG CTGGCATACT CCATCTTGGA 
AACATTGTGT TTGTAGAAAA GGACAACTAT GCTGTCATAC ATGATGATGA GTTTCTTGAT 
TTCCCGTCCT ACCTGCTTGG GCTGGACAAG GAAGCGCTAC GTAAGAAGCT GATCAGCCGT 
CGTATGGACA GTAAATGGGG CGGGAAGGCG GAGCATATAG AGGTCACACT TAACACAGAG 
CAAGCAGCTC ATACCAGAGA TGCTCTAGCT AAGGCTGTCC ATTCAAGACT ATTTGACTTC 
CTCGTTAGTT CCATCAATGG TGCCATGCAG AAGCAGAAGG TAGAGATCAC AATAGGAGTT 
CTGGATATCT ATGGCTTTGA GATCTTTCAA CAGAATGGCT TTGAACAGTT CTGCATTAAC 
TTTGTGAACG AGAAGCTTCA GCAGATCTTT ATAGAACTCA CACTCAAAGC TGAGCAGGAA 
GAGTATGTAG CAGAAGGGAT CAAGTGGAAG AAGATTGATT ACTTCAACAA CAAGATTGTG 
TGTGATCTCA TTGAGAGCAA GGTACCTCCC GGAGTCATGT GCATCCTGGA TGACGTGTGT 
GCGACCATGC ATGCCGTGAG CGATGGTGCC GATGAAAAGC TTCTACAGAA GATGAACAGT 
GCCGTGGGAA CGCATCAGCA CTACGGGGTC TTGAGTGGGG GATTCATTGT ACATCATTAC 
GCTGGCCAGG TGCGGTATAC AGTAGAAGGA TTCTGTGAGA GGAATCGTGA TGTACTCTTC 
ACTGATCTCA TCGAACTTAT GCAGTCAAGT GAGAACTCGT TCTTCATGAC CCTATTTCCC 
GAGAAGATCG CGGGTCAAGC CAAGTCACGA CCCACTACAG CTAGCAGCAA AATCAAAACT 
CAGGCCAACA AGCTAGTAGA CAAACTGATG CAATGTACAC CTCATTACAT CCGCTGTATC 
AAACCAAACG AGACAAAGAG ACCTCAGGAC TGGGAAGAAA AAAGGGTATA TCATCAAGTT 
GAATACCTGG GACTGAAGGA GAACATCAGG GTGAGGCGAG CTGGTTTCGC TTACAGGAGA 
GAATTCGACA AATTTCTCAG ACGGTATGCT ATCTTGACTC CCGAGACATG GCCAAGGTGG 
GGAGGTGATG TCAAGTCTGG GGTCAATCAT CTACTACGCG CTGTCAACAT GGACACTGAT 
GAGTTTCAGC TAGGCAAGAC AAAGGTCTTC ATCAGGAAAC CAGAATCTCT CTTTCTCCTA 
GAAGAACAGA GAGAGAGAAA GTTTGACTTT CATGCTCGGA TCATCCAGAA GGCATTCAGA 
CAATTCAATG CACAGAAGTA CTATCTCAGA CTCAAACAGC AAGCCAGTGA CATCCTGTTC 
GACAAGAAGG AGAGGAGGCG CTACAGTCTC AACCGTAACT TTGTCGGGGA CTACATCGGC 
TTTGAGCACA ATGTATCCCT GCAGGCGTTG GTTGCTAGGA GAGAGAGGAT TGAGTTTGCT 
GATAAGGTCA ACAAGTATGA CAGGAGATTC AAGGTCACGA AGCGAGACCT GCTACTATCA 
GGCAGGAATA TCTATCTGAT TGGTCGAGAG CAGATCAAGA AAGGGCCAGA GAAAGGTCAG 
ATCAGGGACG TGGTCAAAAG GAAGATTCCC CTCAACCATA TACACAGTGT ATCTCTCAGT 
ACTCTTCAGG ATGACATGTT TGTGCTCCAT GTAAGTGAGG AATATGACAG CATCTTAGAG 
TCCACTTTCA AGACTGAATT CCTGACCGTA CTCAACAAGA AGTTCCAAGA GACTCTACAA 
CAGAAACTGA TGGTCAACTT CAATGACTCG ATCGAGTTTT CAGTGAAGAA GGAAGGATGG 
GGTGGAGGAG GAACGAGGAC AGTGAGGTTT GCTCAGGGTC AGGGAGACAC TGCCGTTCTC 
AAGCCGTCCA GCAAGATACT GACCGTTAGC ATTGGTAGTG GACTTCCACG CAACTCAAGA 
CCTGGACTAC GTGATGTCAA GGTGACGAGC AAAGACAGGC AGGCCGGTAA CCGCGGCAAC 
AGTAACCGTG GCAACAGTAA TCGCGGCAAT GGACATGTAT CAAGGAGGCC CGCTCCCAGC 
CAGCCTGCCC CAGGCAAACC CACTGCTCCG TATGCCCTGG TACAGAAGAG AGGGGGTCCT 
ACCAGCCCCA CCAAAGGAGG CATGAAACGT ACTTCAAGTC GAAGGAAGAG TGGAGAGCAG 
CCCCATCTAC CAAAAGGGGG CGCCCAGATC TTCGCCAAAC AGTTCAAGAA CGTGATCAAC 
AGCCAAGACT ACCTACAGAC ACCAGACGCT GGGGTATCAG GGCAAAAACG TAGGACCATA 
AAGGGCAGCC GCCCTGCACC TGGAGCTGGT AGACCCAAGC CCAAACCACA ACCACCAGCC 
CCGTCCAGAC CAAAGTGTCG TACCATCTAC CCCTACGATG CGGGCGATAC GGATGAGTTG 
TCATTCGGAG AAGGAGAGAC TATAGAAATC ATTTTGGAAG ATGCCTCTGG TTGGTGGAGA 
GGTAAGCTAC GAGGGAGAGA GGGACTCTTC CCGGCCAATT ACATTGAAAA GATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene structure in the sea urchin Strongylocentrotus purpuratus based on transcriptome analysis.
Genome research22(10)2079-87(2012 Oct)
Tu Q,Cameron RA,Worley KC,Gibbs RA,Davidson EH

Characterization of sea urchin unconventional myosins and analysis of their patterns of expression during early embryogenesis.
Molecular reproduction and development57(2)111-26(2000 Oct)
Sirotkin V,Seipel S,Krendel M,Bonder EM

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LOC577182 cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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