LOC100890421 cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100890421
Entrez Gene ID	100890421
Full Name	cilia- and flagella-associated protein 70
Synonyms	ttc18
Gene Type	protein-coding
Organism	Strongylocentrotus purpuratus(purple sea urchin)

mRNA and Protein(s)

mRNA	Protein	Name
XM_030998968.1	XP_030854828.1	LOW QUALITY PROTEIN: cilia- and flagella-associated protein 70
XM_011675230.1	XP_011673532.1	LOW QUALITY PROTEIN: cilia- and flagella-associated protein 70

Pathways from BioSystems
Gene Ontology

The following LOC100890421 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100890421 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSd31396	XM_011675230.1 Latest version!	Strongylocentrotus purpuratus cilia- and flagella-associated protein 70 (LOC100890421), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OSd64813	XM_030998968.1 Latest version!	Strongylocentrotus purpuratus cilia- and flagella-associated protein 70 (LOC100890421), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100890421 ( XM_011675230.1 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_011673532.1 Strongylocentrotus purpuratus cilia- and flagella-associated protein 70 (LOC100890421), mRNA.

Price & Availability↑

CloneID	OSd31396
Clone ID Related Accession (Same CDS sequence)	XM_011675230.1
Accession Version	XM_011675230.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3498bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1427040000000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	LOW QUALITY PROTEIN: cilia- and flagella-associated protein 70
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011988356.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Strongylocentrotus purpuratus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases internal stop codons :: corrected 1 genomic stop codon ##RefSeq-Attributes-END##

ATGGCGACCG AAGAGGACCA ACAGAAACAA CGCCCTCNCG AGCATGTGAA CATCACGATC 
TTGAGAGCAC GGAATTTGAA AGGAAGCAAA GGGGACACCC TCAACTCTCT GGTCCGTGTG 
GAGTATGGCA CCAACGTCTT GGGAGAATCT GCCAAAGTGG ACGCAGGGTC AGACCAGTCA 
ACCGAGTATA ATTTCAACGC CAAGTTTGAC TGTAGCTTCG ATGACCCTGC CATCATAGAT 
GAAATTGCTT ACAAACCGGT TGTGGTGACT TTTGTGGAAG TGCTTCCCAA AGAAAAGAAA 
CAGAAAGAAG AGAAGACGTA TCTTCTGGGT CAATGTACTA TTGACCTCTT GCCTCTGGTC 
AAAGGAGAGA CGAGGTATAA GAGCACCCTA CAGATCCATC CCCTGCAAGG CTCACCCCTG 
GAGAGCGTAG GGGCTGATGC CCAGAGGCCT GAGATGGATG TAGTGGTGTA CGTCAATGAC 
CCACTGCTGA CTGAAGACCA GCTGAAGAGT GGCAACCTGA TCACCATCAC GGCGGAGACT 
CTTTACTCCG TCCCTGAATC ATGGAATCCC ACAGGAACAC AGTACATGTA CAATGTGACC 
CTCCCTTTCC CACTCACAGA AGAGAGAGAG TCTGTGGTGG TAATGTCCAA TGGTGTCCTC 
AAAGCAGCTA ACGATAAGGA AATACCGTCC AAACAGAAAA AGTGGCCTGT CCCGGGCGGA 
GCTACCGGTG GCGCTATCTA CATTCCAGAC AAATTTGTAC AACCAAGCGG TGAAGAGCAA 
GAAGATGGGG AGCTGACAAG CAAAGAGGAT CGGGAGTTCA GGACAGAGGC AGAGAATGAA 
AAGAACAGAG TATACTGGAA CTGCGAAAGA AGGGCTTTCT TGGATCCGTC AGCCGTACAG 
AGCTTGCAGA CTAAGATAGC CAAGACAAGA TTCTGGCCTG TGGAGATCAT GAGGATGCCT 
ATGCCTTCAG CTGCTAAAAC AAAGGGAAAG GAGGATGATA ATTCCATATT CTTCCACGGA 
GTGGCTTTCA TCAACTTGGC ACCGCTGCTT TACCCATGAG TGAAAAAGAT TCGAGGAGCG 
TACAGGGTTC CTGCTTTCAC AGAGCAAGAG GTCATGGATA ACTGCAAAAG GAAGGGTGGT 
TTAGCCGATG AGGCTGCTCG CATTGCAACC AGCATGCTCA GGAACGCATC ACCCTTCCCC 
AAGGGAGGAG GGAAAGGGGG CAAAGAGGAG AAAGGAGCCA AAGACAAAGA TAAAGAAAAG 
GACAAGGATG CTGCTACAAA GAAGCAACCC TCCCAGATGC AGAAGCTCGG TGCCCCAGGG 
ACGGAAGGGG CATCGGACCC AGACGCCCCG CCCCCACAGA ATCTAGAGGG CATGCAGTAC 
GACGAGGCAC GCACCTACAT CATCGTGGAG TTTGAGCTAG AGTCACCCAT TGTTCCAAAG 
AGGGCCCCTG AAGAGTTGGC AAAGAGAGTT GCTGAGATTG TTCCCCCAAG GCCTCTCTTC 
CCCCGGAGGA CCAATGGAGC ACAGAAGGCT GTGGAAGACT TCCATCAACA GGTGGCCGGC 
ATCAGCAAGC TTGTCCTGGA AGAATTCAGA GGATTGTTTG GAGATCTAGG TGATCTGAGC 
AAGAGCAGCG GTGAGGCTCA GGATGAAAGA AGAAGGAAAC TAATCTATGA GCTCAACACT 
ACCGGAAAAT ACTTTGCATT CAAGGAACAA TTAAAGCATT CTGTGGTGAA GATTGTGAGA 
GAGAAGTACT TACACACAAG TACTTTTGAA GACAAAGAGG AATTACAGGC ATTCCTGAGT 
GAGCTCTACG TCTTCCTGGT GGACCAGATG CATGTTGGGG TTGGGAAGGT GCTCTCATTG 
GAGGATGAGA CCCCGGTCCC CGCCCCTCTC ACGGACTCCG CCCAGCTCAA GCACTTTGCC 
AGAGAAGCAG AGGTCAATGA GAACTTTGAA CTGGCTTCGA AATATTATCA GGAGCGCATT 
GCAAGGAACA GAAATGATGC TGACCATTGG TTTGACTATG GTTCCTTCTG TCTCTACATC 
AATGATATTG CAAAGGCTGA GGAGTGCTTC AAGGAGACCA TCTCTATCAA CCAGCAGCAT 
CTCCACGGTC TGCTGCTCTA CGGGGTGGTG TGTGCTATGG AGGAAGGCAA TGAAGCGGCA 
GAGACGTTCT TTGAGGCTGC TACCTGTGTT GATCCCTCTA GTATCTTGGC CTGGATCATG 
CTGGGTCTCT ACTATGATGG CATTGACTGC GACATCCAGG CTGAGTATGC CTTCATGGAG 
GCGCGACGTC TCAACATGGC TCATGCTGCT GCCAAGCAGA GAGAGTGTAA GGTGGCAGCC 
TCTGCAGCTG CTGCTGCAGG CACTGTCATT GAGGAGGAGG AGGAGGAGGG GGAAGCCTCG 
CCCCCAGGGG AGGGGGACGA CCAACAGTCC GCCCTTGGGG AGGGTGATGG ATCGAAGAAC 
GGAGAAGATA TGTCCAGTGA AAAAGGAAGC ACTGTCCCAA CGGTCAATAA AATCCCGGCT 
ACTCCGACCG ACGTTTCTAA GACCAAATCT CCAGCGGTCA AGCCTGCTCC TCCGGCCGCT 
GCTCAGAAGA AGAGGATGTC CATCTCATCC AAAGACTCAT TGCAGTCTCT TAATCAAATT 
GGCCAGAAAG ATCCGCAGCA GGCCAAGCGA ACGACCAGCG CCACCAGCCA GCGAGCGGGC 
AGCATCAAGG CCGTCAGTCG GCCGGGCTCT GGGGTGGCTG CTGAACCACA AGCGGAAGGG 
GAGGAGGAAG GCGCGGTTGC CCAGAAGGAA AGTCCTCCGG TACCGGAGAC CACCATCTTC 
ATGCTTGCCG TGGAGTTCTT GCTGGAGGTC AAAGGCATGC AGTTCTGTGA GCGTGCCCTG 
GCCCATGAGT TGCTGGACCT GATGGGTGGA CCCACTACGG CCTATCACGT AGCCCTGGCC 
AGACTCAAGC TACAGAAGAA AGCCTTGGAG GAAGCCAACG AGAGCCTCAA CCAAGCACTG 
GTCCTAAACT ATCAGGACCC TGATGCCTGG TCCTTCTTGG GCCATCTTCG CTACCTCCAG 
GGTAACTTTG AGGAGGCCAA GGACTGCTAC GAGAGGACCC TCTCCTTCGT GACTGACGCC 
CGGGAGATGC ATGCCATCTA CCTTCGTCTA GCATCCATCT ATTTACAAGA TGGACAGTAT 
GATAAATCCA AGGTAACATT CCTGCTTGCC TGTAAGAACT CGCCGTCCTG TATATCCTGG 
CTTGGTGTGG GCATAGCTTG CTTCAGGCTT GGGGAACTGT CAGAGGCGGA AGATGCCCTC 
TGCGAGGCCA ACATCCTGAA CAACAGCGAC CCTGAGGTCT GGGGCTACCT GTCCCTGGTC 
TGCCTGAGGA CCGCCCGCCA GCAGGAGGCA GAGCAAGCCT ACAAGTATGC CCTCAAGGTC 
GGCCTGGCTG ACGAGGGTCT TCTGGAGGAG ATCCACAAGA CGCAGGAGGA GGTGGGCTTC 
GGTAACCCCC AGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011673532.1
CDS	3..3500
Translation	MATEEDQQKQRPnullEHVNITILRARNLKGSKGDTLNSLVRVEYGTNVLGESAKVDAGSDQSTEYNFNAKFDCSFDDPAIIDEIAYKPVVVTFVEVLPKEKKQKEEKTYLLGQCTIDLLPLVKGETRYKSTLQIHPLQGSPLESVGADAQRPEMDVVVYVNDPLLTEDQLKSGNLITITAETLYSVPESWNPTGTQYMYNVTLPFPLTEERESVVVMSNGVLKAANDKEIPSKQKKWPVPGGATGGAIYIPDKFVQPSGEEQEDGELTSKEDREFRTEAENEKNRVYWNCERRAFLDPSAVQSLQTKIAKTRFWPVEIMRMPMPSAAKTKGKEDDNSIFFHGVAFINLAPLLYP*VKKIRGAYRVPAFTEQEVMDNCKRKGGLADEAARIATSMLRNASPFPKGGGKGGKEEKGAKDKDKEKDKDAATKKQPSQMQKLGAPGTEGASDPDAPPPQNLEGMQYDEARTYIIVEFELESPIVPKRAPEELAKRVAEIVPPRPLFPRRTNGAQKAVEDFHQQVAGISKLVLEEFRGLFGDLGDLSKSSGEAQDERRRKLIYELNTTGKYFAFKEQLKHSVVKIVREKYLHTSTFEDKEELQAFLSELYVFLVDQMHVGVGKVLSLEDETPVPAPLTDSAQLKHFAREAEVNENFELASKYYQERIARNRNDADHWFDYGSFCLYINDIAKAEECFKETISINQQHLHGLLLYGVVCAMEEGNEAAETFFEAATCVDPSSILAWIMLGLYYDGIDCDIQAEYAFMEARRLNMAHAAAKQRECKVAASAAAAAGTVIEEEEEEGEASPPGEGDDQQSALGEGDGSKNGEDMSSEKGSTVPTVNKIPATPTDVSKTKSPAVKPAPPAAAQKKRMSISSKDSLQSLNQIGQKDPQQAKRTTSATSQRAGSIKAVSRPGSGVAAEPQAEGEEEGAVAQKESPPVPETTIFMLAVEFLLEVKGMQFCERALAHELLDLMGGPTTAYHVALARLKLQKKALEEANESLNQALVLNYQDPDAWSFLGHLRYLQGNFEEAKDCYERTLSFVTDAREMHAIYLRLASIYLQDGQYDKSKVTFLLACKNSPSCISWLGVGIACFRLGELSEAEDALCEANILNNSDPEVWGYLSLVCLRTARQQEAEQAYKYALKVGLADEGLLEEIHKTQEEVGFGNPQF

Target ORF information:

RefSeq Version	XM_011675230.1
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus cilia- and flagella-associated protein 70 (LOC100890421), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011675230.1

ORF Insert Sequence:

ATGGCGACCG AAGAGGACCA ACAGAAACAA CGCCCTCNCG AGCATGTGAA CATCACGATC 
TTGAGAGCAC GGAATTTGAA AGGAAGCAAA GGGGACACCC TCAACTCTCT GGTCCGTGTG 
GAGTATGGCA CCAACGTCTT GGGAGAATCT GCCAAAGTGG ACGCAGGGTC AGACCAGTCA 
ACCGAGTATA ATTTCAACGC CAAGTTTGAC TGTAGCTTCG ATGACCCTGC CATCATAGAT 
GAAATTGCTT ACAAACCGGT TGTGGTGACT TTTGTGGAAG TGCTTCCCAA AGAAAAGAAA 
CAGAAAGAAG AGAAGACGTA TCTTCTGGGT CAATGTACTA TTGACCTCTT GCCTCTGGTC 
AAAGGAGAGA CGAGGTATAA GAGCACCCTA CAGATCCATC CCCTGCAAGG CTCACCCCTG 
GAGAGCGTAG GGGCTGATGC CCAGAGGCCT GAGATGGATG TAGTGGTGTA CGTCAATGAC 
CCACTGCTGA CTGAAGACCA GCTGAAGAGT GGCAACCTGA TCACCATCAC GGCGGAGACT 
CTTTACTCCG TCCCTGAATC ATGGAATCCC ACAGGAACAC AGTACATGTA CAATGTGACC 
CTCCCTTTCC CACTCACAGA AGAGAGAGAG TCTGTGGTGG TAATGTCCAA TGGTGTCCTC 
AAAGCAGCTA ACGATAAGGA AATACCGTCC AAACAGAAAA AGTGGCCTGT CCCGGGCGGA 
GCTACCGGTG GCGCTATCTA CATTCCAGAC AAATTTGTAC AACCAAGCGG TGAAGAGCAA 
GAAGATGGGG AGCTGACAAG CAAAGAGGAT CGGGAGTTCA GGACAGAGGC AGAGAATGAA 
AAGAACAGAG TATACTGGAA CTGCGAAAGA AGGGCTTTCT TGGATCCGTC AGCCGTACAG 
AGCTTGCAGA CTAAGATAGC CAAGACAAGA TTCTGGCCTG TGGAGATCAT GAGGATGCCT 
ATGCCTTCAG CTGCTAAAAC AAAGGGAAAG GAGGATGATA ATTCCATATT CTTCCACGGA 
GTGGCTTTCA TCAACTTGGC ACCGCTGCTT TACCCATGAG TGAAAAAGAT TCGAGGAGCG 
TACAGGGTTC CTGCTTTCAC AGAGCAAGAG GTCATGGATA ACTGCAAAAG GAAGGGTGGT 
TTAGCCGATG AGGCTGCTCG CATTGCAACC AGCATGCTCA GGAACGCATC ACCCTTCCCC 
AAGGGAGGAG GGAAAGGGGG CAAAGAGGAG AAAGGAGCCA AAGACAAAGA TAAAGAAAAG 
GACAAGGATG CTGCTACAAA GAAGCAACCC TCCCAGATGC AGAAGCTCGG TGCCCCAGGG 
ACGGAAGGGG CATCGGACCC AGACGCCCCG CCCCCACAGA ATCTAGAGGG CATGCAGTAC 
GACGAGGCAC GCACCTACAT CATCGTGGAG TTTGAGCTAG AGTCACCCAT TGTTCCAAAG 
AGGGCCCCTG AAGAGTTGGC AAAGAGAGTT GCTGAGATTG TTCCCCCAAG GCCTCTCTTC 
CCCCGGAGGA CCAATGGAGC ACAGAAGGCT GTGGAAGACT TCCATCAACA GGTGGCCGGC 
ATCAGCAAGC TTGTCCTGGA AGAATTCAGA GGATTGTTTG GAGATCTAGG TGATCTGAGC 
AAGAGCAGCG GTGAGGCTCA GGATGAAAGA AGAAGGAAAC TAATCTATGA GCTCAACACT 
ACCGGAAAAT ACTTTGCATT CAAGGAACAA TTAAAGCATT CTGTGGTGAA GATTGTGAGA 
GAGAAGTACT TACACACAAG TACTTTTGAA GACAAAGAGG AATTACAGGC ATTCCTGAGT 
GAGCTCTACG TCTTCCTGGT GGACCAGATG CATGTTGGGG TTGGGAAGGT GCTCTCATTG 
GAGGATGAGA CCCCGGTCCC CGCCCCTCTC ACGGACTCCG CCCAGCTCAA GCACTTTGCC 
AGAGAAGCAG AGGTCAATGA GAACTTTGAA CTGGCTTCGA AATATTATCA GGAGCGCATT 
GCAAGGAACA GAAATGATGC TGACCATTGG TTTGACTATG GTTCCTTCTG TCTCTACATC 
AATGATATTG CAAAGGCTGA GGAGTGCTTC AAGGAGACCA TCTCTATCAA CCAGCAGCAT 
CTCCACGGTC TGCTGCTCTA CGGGGTGGTG TGTGCTATGG AGGAAGGCAA TGAAGCGGCA 
GAGACGTTCT TTGAGGCTGC TACCTGTGTT GATCCCTCTA GTATCTTGGC CTGGATCATG 
CTGGGTCTCT ACTATGATGG CATTGACTGC GACATCCAGG CTGAGTATGC CTTCATGGAG 
GCGCGACGTC TCAACATGGC TCATGCTGCT GCCAAGCAGA GAGAGTGTAA GGTGGCAGCC 
TCTGCAGCTG CTGCTGCAGG CACTGTCATT GAGGAGGAGG AGGAGGAGGG GGAAGCCTCG 
CCCCCAGGGG AGGGGGACGA CCAACAGTCC GCCCTTGGGG AGGGTGATGG ATCGAAGAAC 
GGAGAAGATA TGTCCAGTGA AAAAGGAAGC ACTGTCCCAA CGGTCAATAA AATCCCGGCT 
ACTCCGACCG ACGTTTCTAA GACCAAATCT CCAGCGGTCA AGCCTGCTCC TCCGGCCGCT 
GCTCAGAAGA AGAGGATGTC CATCTCATCC AAAGACTCAT TGCAGTCTCT TAATCAAATT 
GGCCAGAAAG ATCCGCAGCA GGCCAAGCGA ACGACCAGCG CCACCAGCCA GCGAGCGGGC 
AGCATCAAGG CCGTCAGTCG GCCGGGCTCT GGGGTGGCTG CTGAACCACA AGCGGAAGGG 
GAGGAGGAAG GCGCGGTTGC CCAGAAGGAA AGTCCTCCGG TACCGGAGAC CACCATCTTC 
ATGCTTGCCG TGGAGTTCTT GCTGGAGGTC AAAGGCATGC AGTTCTGTGA GCGTGCCCTG 
GCCCATGAGT TGCTGGACCT GATGGGTGGA CCCACTACGG CCTATCACGT AGCCCTGGCC 
AGACTCAAGC TACAGAAGAA AGCCTTGGAG GAAGCCAACG AGAGCCTCAA CCAAGCACTG 
GTCCTAAACT ATCAGGACCC TGATGCCTGG TCCTTCTTGG GCCATCTTCG CTACCTCCAG 
GGTAACTTTG AGGAGGCCAA GGACTGCTAC GAGAGGACCC TCTCCTTCGT GACTGACGCC 
CGGGAGATGC ATGCCATCTA CCTTCGTCTA GCATCCATCT ATTTACAAGA TGGACAGTAT 
GATAAATCCA AGGTAACATT CCTGCTTGCC TGTAAGAACT CGCCGTCCTG TATATCCTGG 
CTTGGTGTGG GCATAGCTTG CTTCAGGCTT GGGGAACTGT CAGAGGCGGA AGATGCCCTC 
TGCGAGGCCA ACATCCTGAA CAACAGCGAC CCTGAGGTCT GGGGCTACCT GTCCCTGGTC 
TGCCTGAGGA CCGCCCGCCA GCAGGAGGCA GAGCAAGCCT ACAAGTATGC CCTCAAGGTC 
GGCCTGGCTG ACGAGGGTCT TCTGGAGGAG ATCCACAAGA CGCAGGAGGA GGTGGGCTTC 
GGTAACCCCC AGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100890421 ( XM_030998968.1 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_030854828.1 Strongylocentrotus purpuratus cilia- and flagella-associated protein 70 (LOC100890421), mRNA.

Price & Availability↑

CloneID	OSd64813
Clone ID Related Accession (Same CDS sequence)	XM_030998968.1
Accession Version	XM_030998968.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3498bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569427200000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	LOW QUALITY PROTEIN: cilia- and flagella-associated protein 70
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_022145611.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Strongylocentrotus purpuratus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGGCGACCG AAGAGGACCA ACAGAAACAA CGTCCTCCCG AGCATGTAAA CATCACGATC 
TTGAGAGCAC GGAATTTGAA AGGAAGCAAA GGGGACACCC TCAACTCTCT GGTCCGTGTG 
GAGTATGGCA CCAACGTCTT GGGAGAATCT GCCAAAGTGG ACGCAGGGTC AGACCAGTCA 
ACCGAGTATA ATTTCAACGC CAAGTTTGAC TGTAGCTTCG ATGACCCTGC CATCATAGAT 
GAAATTGCTT ACAAACCGGT TGTGGTGACT TTTGTGGAAG TGCTTCCCNA AGAAAAGAAA 
CAGAAAGAAG AGAAGACGTA TCTTCTGGGT CAATGTACTA TTGACCTCTT GCCTCTGGTC 
AAAGGAGAGA CGAGGTATAA GAGCACCCTA CAGATCCATC CCCTGCAAGG CTCACCCCTG 
GAGAGCGTAG GGGCTGATGC CCAGAGGCCT GAGATGGATG TAGTGGTGTA CGTCAATGAC 
CCACTGCTGA CTGAAGACCA GCTGAAGAGT GGCAACCTGA TCACCATCAC GGCGGAGACT 
CTTTACTCCG TCCCTGAATC ATGGAATCCC ACAGGAACAC AGTACATGTA CAATGTGACC 
CTCCCTTTCC CACTCACAGA AGAGAGAGAG TCTGTGGTGG TAATGTCCAA TGGTGTCCTC 
AAAGCAGCTA ACGATAAGGA AATACCGTCC AAACAGAAAA AGTGGCCTGT CCCGGGCGGA 
GCTACCGGTG GCGCTATCTA CATTCCAGAC AAATTTGTAC AACCAAGCGG TGAAGAGCAA 
GAAGATGGGG AGCTGACAAG CAAAGAGGAT CGGGAGTTCA GGACAGAGGC AGAGAATGAA 
AAGAACAGAG TATACTGGAA CTGCGAAAGA AGGGCTTTCT TGGATCCGTC AGCCGTACAG 
AGCTTGCAGA CTAAGATAGC CAAGACAAGA TTCTGGCCTG TGGAGATCAT GAGGATGCCT 
ATGCCTTCAG CTGCTAAAAC AAAGGGAAAG GAGGATGATA ATTCCATATT CTTCCACGGA 
GTGGCTTTCA TCAACTTGGC ACCGCTGCTT TACCCAGGAG TGAAAAAGAT TCGAGGAGCG 
TACAGGGTTC ATGCTTTCAC AGAGCAAGAG GTCATGGAAA AGTGCAAAAG GAAGGGTGGT 
TTAGCCGATG AGGCTGCTCG CATTGCAACC AGCATGCTCA GGAACGCATC ACCCTTCCCC 
AAGGGAGGAG GGAAAGGGGG CAAAGAGGAG AAAGGAGCCA AAGACAAAGA TAAAGAAAAG 
GACAAGGATG CTGCTACAAA GAAGCAACCC TCCCAGATGC AGAAGCTTGG TGCCCCAGGG 
ACGGAGGGGG CATCGGATCC AGACGCCCCG CCCCCACAGA ACATAGAGGG CATGCAGTAT 
GATGAGGCAC GCACCTACAT CATTGTGGAG TTTGAGCTGG AGTCACCCAT TGTTCCAAAG 
AGGGCCCCTG AAGAGTTGGC AAAGAGAGTT GCTGAGATTG TTCCCCCAAG GCCTCTCTTC 
CCCCGGAGGA CCAACGGAGC ACAGAAGGCT GTGGAAGACT TCCATCAACA GGTGGCCGGC 
ATCAGCAAGC TTGTACTGGA AGAATTCAGA GGATTGTTTG GAGATCTAGG TGATCTGAGC 
AAGAGCAGCG GTGAGGCTCA GGATGAAAGA AGAAGGAAAC TAATCTATGA GCTCAACACT 
ACCGGAAAAT ACTTTGCATT CAAGGAACAA TTAAAGCATT CTGTGGTGAA GATTGTGAGA 
GAGAAGTACT TACACACAAG TACTTTTGAA GACAAAGAGG AATTACAGGC ATTCCTGAGT 
GAGCTCTACG TCTTCCTGGT GGACCAGATG CATGTTGGGG TCGGGAAGGT GCTATCGCTG 
GAGGACGAGA CCCCTGTCCC CGCCCCTCTC ACCGACTCCG CCCAGCTCAA GCACTTTGCC 
AGAGAGGCAG AGGTCAATGA GAACTTTGAA CTGGCATCAA AATATTATCA GGAGCGCATT 
GCAAGGAACA GGAATGATGC TGACCATTGG TTTGACTATG GATCCTTCTG TCTGTACATC 
AATGATATTG CAAAGGCTGA GGAGTGCTTC AAGGAGACCA TCTCTATCAA CCAGCAGCAT 
CTCCACGGTC TGCTGCTCTA CGGGGTGGTG TGTGCTATGG AGGAAGGCAA CGAAGCAGCA 
GAGACTTTCT TCGAGGCTGC TACCTGTGTG GATCCCTCTA GTATCTTGGC CTGGATCATG 
CTAGGTCTCT ACTATGATGG CATTGACTGC GACATCCAGG CTGAGTATGC CTTCATGGAG 
GCGCGACGTC TCAACATGGC TCATGCTGCT GCCAAGCAGA GAGAGTGTAA GGTGGCAGCC 
TCTGCAGCTG CTGCTGCAGG CACTGTCATT GAGGAGGAGG AGGAGGAGGG GGAAGCCTCG 
CCCCCAGGGG AGGGGGACGA CCAACAGTCC GCCCTTGGGG AGGGTGATGG ATCGAAGAAC 
GGAGAAGATA TGTCCAGTGA AAAAGGAAGC ACTGTCCCAA CGGTCAATAA AATCCCGGCT 
ACTCCGACCG ACGTTTCTAA GACCAAATCT CCAGCGGTCA AGCCTGCTCC TCCGGCCGCT 
GCTCAGAAGA AGAGGATGTC CATCTCATCC AAAGACTCAT TGCAGTCTCT TAATCAAATT 
GGCCAGAAAG ATCCGCAGCA GGCCAAGCGA ACGACCAGCG CCACCAGCCA GCGAGCGGGC 
AGCATCAAGG CCGTCAGTCG GCCGGGCTCT GGGGTGGCTG CTGAACCACA AGCGGAAGGG 
GAGGAGGAAG GCGCGGTTGC CCAGAAGGAA AGTCCTCCGG TACCGGAGAC CACCATCTTC 
ATGCTTGCCG TGGAGTTCTT GCTGGAGGTC AAAGGCATGC AGTTCTGTGA GCGTGCCCTG 
GCCCATGAGT TGCTGGACCT GATGGGTGGA CCCACTACGG CCTATCACGT AGCCCTGGCC 
AGACTCAAGC TACAGAAGAA AGCCTTGGAG GAAGCCAACG AGAGCCTCAA CCAAGCACTG 
GTCCTAAACT ATCAGGACCC TGATGCCTGG TCCTTCTTGG GCCATCTTCG CTACCTCCAG 
GGTAACTTTG AGGAGGCCAA GGACTGCTAC GAGAGGACCC TCTCCTTCGT GACTGACGCC 
CGGGAGATGC ATGCCATCTA CCTTCGTCTA GCATCCATCT ATTTACAAGA TGGACAGTAT 
GATAAATCCA AGGTAACATT CCTGCTTGCC TGTAAGAACT CGCCGTCCTG TATATCCTGG 
CTTGGTGTGG GCATAGCTTG CTTCAGGCTT GGGGAACTGT CAGAGGCGGA AGATGCCCTC 
TGCGAGGCCA ACATCCTGAA CAACAGCGAC CCTGAGGTCT GGGGCTACCT GTCCCTGGTC 
TGCCTGAGGA CCGCCCGCCA GCAGGAGGCA GAGCAAGCCT ACAAGTATGC CCTCAAGGTC 
GGCCTGGCTG ACGAGGGTCT TCTGGAGGAG ATCCACAAGA CGCAGGAGGA GGTGGGCTTC 
GGTAACCCCC AGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030854828.1
CDS	160..3657
Translation	MATEEDQQKQRPPEHVNITILRARNLKGSKGDTLNSLVRVEYGTNVLGESAKVDAGSDQSTEYNFNAKFDCSFDDPAIIDEIAYKPVVVTFVEVLPnullEKKQKEEKTYLLGQCTIDLLPLVKGETRYKSTLQIHPLQGSPLESVGADAQRPEMDVVVYVNDPLLTEDQLKSGNLITITAETLYSVPESWNPTGTQYMYNVTLPFPLTEERESVVVMSNGVLKAANDKEIPSKQKKWPVPGGATGGAIYIPDKFVQPSGEEQEDGELTSKEDREFRTEAENEKNRVYWNCERRAFLDPSAVQSLQTKIAKTRFWPVEIMRMPMPSAAKTKGKEDDNSIFFHGVAFINLAPLLYPGVKKIRGAYRVHAFTEQEVMEKCKRKGGLADEAARIATSMLRNASPFPKGGGKGGKEEKGAKDKDKEKDKDAATKKQPSQMQKLGAPGTEGASDPDAPPPQNIEGMQYDEARTYIIVEFELESPIVPKRAPEELAKRVAEIVPPRPLFPRRTNGAQKAVEDFHQQVAGISKLVLEEFRGLFGDLGDLSKSSGEAQDERRRKLIYELNTTGKYFAFKEQLKHSVVKIVREKYLHTSTFEDKEELQAFLSELYVFLVDQMHVGVGKVLSLEDETPVPAPLTDSAQLKHFAREAEVNENFELASKYYQERIARNRNDADHWFDYGSFCLYINDIAKAEECFKETISINQQHLHGLLLYGVVCAMEEGNEAAETFFEAATCVDPSSILAWIMLGLYYDGIDCDIQAEYAFMEARRLNMAHAAAKQRECKVAASAAAAAGTVIEEEEEEGEASPPGEGDDQQSALGEGDGSKNGEDMSSEKGSTVPTVNKIPATPTDVSKTKSPAVKPAPPAAAQKKRMSISSKDSLQSLNQIGQKDPQQAKRTTSATSQRAGSIKAVSRPGSGVAAEPQAEGEEEGAVAQKESPPVPETTIFMLAVEFLLEVKGMQFCERALAHELLDLMGGPTTAYHVALARLKLQKKALEEANESLNQALVLNYQDPDAWSFLGHLRYLQGNFEEAKDCYERTLSFVTDAREMHAIYLRLASIYLQDGQYDKSKVTFLLACKNSPSCISWLGVGIACFRLGELSEAEDALCEANILNNSDPEVWGYLSLVCLRTARQQEAEQAYKYALKVGLADEGLLEEIHKTQEEVGFGNPQF

Target ORF information:

RefSeq Version	XM_030998968.1
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus cilia- and flagella-associated protein 70 (LOC100890421), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030998968.1

ORF Insert Sequence:

ATGGCGACCG AAGAGGACCA ACAGAAACAA CGTCCTCCCG AGCATGTAAA CATCACGATC 
TTGAGAGCAC GGAATTTGAA AGGAAGCAAA GGGGACACCC TCAACTCTCT GGTCCGTGTG 
GAGTATGGCA CCAACGTCTT GGGAGAATCT GCCAAAGTGG ACGCAGGGTC AGACCAGTCA 
ACCGAGTATA ATTTCAACGC CAAGTTTGAC TGTAGCTTCG ATGACCCTGC CATCATAGAT 
GAAATTGCTT ACAAACCGGT TGTGGTGACT TTTGTGGAAG TGCTTCCCNA AGAAAAGAAA 
CAGAAAGAAG AGAAGACGTA TCTTCTGGGT CAATGTACTA TTGACCTCTT GCCTCTGGTC 
AAAGGAGAGA CGAGGTATAA GAGCACCCTA CAGATCCATC CCCTGCAAGG CTCACCCCTG 
GAGAGCGTAG GGGCTGATGC CCAGAGGCCT GAGATGGATG TAGTGGTGTA CGTCAATGAC 
CCACTGCTGA CTGAAGACCA GCTGAAGAGT GGCAACCTGA TCACCATCAC GGCGGAGACT 
CTTTACTCCG TCCCTGAATC ATGGAATCCC ACAGGAACAC AGTACATGTA CAATGTGACC 
CTCCCTTTCC CACTCACAGA AGAGAGAGAG TCTGTGGTGG TAATGTCCAA TGGTGTCCTC 
AAAGCAGCTA ACGATAAGGA AATACCGTCC AAACAGAAAA AGTGGCCTGT CCCGGGCGGA 
GCTACCGGTG GCGCTATCTA CATTCCAGAC AAATTTGTAC AACCAAGCGG TGAAGAGCAA 
GAAGATGGGG AGCTGACAAG CAAAGAGGAT CGGGAGTTCA GGACAGAGGC AGAGAATGAA 
AAGAACAGAG TATACTGGAA CTGCGAAAGA AGGGCTTTCT TGGATCCGTC AGCCGTACAG 
AGCTTGCAGA CTAAGATAGC CAAGACAAGA TTCTGGCCTG TGGAGATCAT GAGGATGCCT 
ATGCCTTCAG CTGCTAAAAC AAAGGGAAAG GAGGATGATA ATTCCATATT CTTCCACGGA 
GTGGCTTTCA TCAACTTGGC ACCGCTGCTT TACCCAGGAG TGAAAAAGAT TCGAGGAGCG 
TACAGGGTTC ATGCTTTCAC AGAGCAAGAG GTCATGGAAA AGTGCAAAAG GAAGGGTGGT 
TTAGCCGATG AGGCTGCTCG CATTGCAACC AGCATGCTCA GGAACGCATC ACCCTTCCCC 
AAGGGAGGAG GGAAAGGGGG CAAAGAGGAG AAAGGAGCCA AAGACAAAGA TAAAGAAAAG 
GACAAGGATG CTGCTACAAA GAAGCAACCC TCCCAGATGC AGAAGCTTGG TGCCCCAGGG 
ACGGAGGGGG CATCGGATCC AGACGCCCCG CCCCCACAGA ACATAGAGGG CATGCAGTAT 
GATGAGGCAC GCACCTACAT CATTGTGGAG TTTGAGCTGG AGTCACCCAT TGTTCCAAAG 
AGGGCCCCTG AAGAGTTGGC AAAGAGAGTT GCTGAGATTG TTCCCCCAAG GCCTCTCTTC 
CCCCGGAGGA CCAACGGAGC ACAGAAGGCT GTGGAAGACT TCCATCAACA GGTGGCCGGC 
ATCAGCAAGC TTGTACTGGA AGAATTCAGA GGATTGTTTG GAGATCTAGG TGATCTGAGC 
AAGAGCAGCG GTGAGGCTCA GGATGAAAGA AGAAGGAAAC TAATCTATGA GCTCAACACT 
ACCGGAAAAT ACTTTGCATT CAAGGAACAA TTAAAGCATT CTGTGGTGAA GATTGTGAGA 
GAGAAGTACT TACACACAAG TACTTTTGAA GACAAAGAGG AATTACAGGC ATTCCTGAGT 
GAGCTCTACG TCTTCCTGGT GGACCAGATG CATGTTGGGG TCGGGAAGGT GCTATCGCTG 
GAGGACGAGA CCCCTGTCCC CGCCCCTCTC ACCGACTCCG CCCAGCTCAA GCACTTTGCC 
AGAGAGGCAG AGGTCAATGA GAACTTTGAA CTGGCATCAA AATATTATCA GGAGCGCATT 
GCAAGGAACA GGAATGATGC TGACCATTGG TTTGACTATG GATCCTTCTG TCTGTACATC 
AATGATATTG CAAAGGCTGA GGAGTGCTTC AAGGAGACCA TCTCTATCAA CCAGCAGCAT 
CTCCACGGTC TGCTGCTCTA CGGGGTGGTG TGTGCTATGG AGGAAGGCAA CGAAGCAGCA 
GAGACTTTCT TCGAGGCTGC TACCTGTGTG GATCCCTCTA GTATCTTGGC CTGGATCATG 
CTAGGTCTCT ACTATGATGG CATTGACTGC GACATCCAGG CTGAGTATGC CTTCATGGAG 
GCGCGACGTC TCAACATGGC TCATGCTGCT GCCAAGCAGA GAGAGTGTAA GGTGGCAGCC 
TCTGCAGCTG CTGCTGCAGG CACTGTCATT GAGGAGGAGG AGGAGGAGGG GGAAGCCTCG 
CCCCCAGGGG AGGGGGACGA CCAACAGTCC GCCCTTGGGG AGGGTGATGG ATCGAAGAAC 
GGAGAAGATA TGTCCAGTGA AAAAGGAAGC ACTGTCCCAA CGGTCAATAA AATCCCGGCT 
ACTCCGACCG ACGTTTCTAA GACCAAATCT CCAGCGGTCA AGCCTGCTCC TCCGGCCGCT 
GCTCAGAAGA AGAGGATGTC CATCTCATCC AAAGACTCAT TGCAGTCTCT TAATCAAATT 
GGCCAGAAAG ATCCGCAGCA GGCCAAGCGA ACGACCAGCG CCACCAGCCA GCGAGCGGGC 
AGCATCAAGG CCGTCAGTCG GCCGGGCTCT GGGGTGGCTG CTGAACCACA AGCGGAAGGG 
GAGGAGGAAG GCGCGGTTGC CCAGAAGGAA AGTCCTCCGG TACCGGAGAC CACCATCTTC 
ATGCTTGCCG TGGAGTTCTT GCTGGAGGTC AAAGGCATGC AGTTCTGTGA GCGTGCCCTG 
GCCCATGAGT TGCTGGACCT GATGGGTGGA CCCACTACGG CCTATCACGT AGCCCTGGCC 
AGACTCAAGC TACAGAAGAA AGCCTTGGAG GAAGCCAACG AGAGCCTCAA CCAAGCACTG 
GTCCTAAACT ATCAGGACCC TGATGCCTGG TCCTTCTTGG GCCATCTTCG CTACCTCCAG 
GGTAACTTTG AGGAGGCCAA GGACTGCTAC GAGAGGACCC TCTCCTTCGT GACTGACGCC 
CGGGAGATGC ATGCCATCTA CCTTCGTCTA GCATCCATCT ATTTACAAGA TGGACAGTAT 
GATAAATCCA AGGTAACATT CCTGCTTGCC TGTAAGAACT CGCCGTCCTG TATATCCTGG 
CTTGGTGTGG GCATAGCTTG CTTCAGGCTT GGGGAACTGT CAGAGGCGGA AGATGCCCTC 
TGCGAGGCCA ACATCCTGAA CAACAGCGAC CCTGAGGTCT GGGGCTACCT GTCCCTGGTC 
TGCCTGAGGA CCGCCCGCCA GCAGGAGGCA GAGCAAGCCT ACAAGTATGC CCTCAAGGTC 
GGCCTGGCTG ACGAGGGTCT TCTGGAGGAG ATCCACAAGA CGCAGGAGGA GGTGGGCTTC 
GGTAACCCCC AGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC100890421 cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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