LOC8073495 cDNA ORF clone, Sorghum bicolor(sorghum)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC8073495
Entrez Gene ID	8073495
Full Name	nucleolar protein 6
Gene Type	protein-coding
Organism	Sorghum bicolor(sorghum)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002441835.1	XP_002441880.1	hypothetical protein
XM_021446402.1	XP_021302077.1	nucleolar protein 6

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The Sorghum bicolor genome and the diversification of grasses.
Paterson AH, Bowers JE, Bruggmann R, Dubchak I, Grimwood J, Gundlach H, Haberer G, Hellsten U, Mitros T, Poliakov A, Schmutz J, Spannagl M, Tang H, Wang X, Wicker T, Bharti AK, Chapman J, Feltus FA, Gowik U, Grigoriev IV, Lyons E, Maher CA, Martis M, Narechania A, Otillar RP, Penning BW, Salamov AA, Wang Y, Zhang L, Carpita NC, Freeling M, Gingle AR, Hash CT, Keller B, Klein P, Kresovich S, McCann MC, Ming R, Peterson DG, Mehboob-ur-Rahman, Ware D, Westhoff P, Mayer KF, Messing J, Rokhsar DS
Nature457(7229)551-6(2009 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC8073495 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC8073495 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc104044	XM_021446402.1 Latest version!	Sorghum bicolor nucleolar protein 6 (LOC8073495), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OSc12027	XM_002441835.1 Latest version!	Sorghum bicolor hypothetical protein, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC8073495 ( XM_021446402.1 ) cDNA ORF clone, Sorghum bicolor(sorghum) -> XP_021302077.1 Sorghum bicolor nucleolar protein 6 (LOC8073495), mRNA.

Price & Availability↑

CloneID	OSc104044
Clone ID Related Accession (Same CDS sequence)	XM_021446402.1
Accession Version	XM_021446402.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3162bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497196800000
Organism	Sorghum bicolor(sorghum)
Product	nucleolar protein 6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_012877.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sorghum bicolor Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACCA CCTCCGCCGC CTCCGAGTAC TCCGTCGACC GCAAGCTCTC CGCGCTCGTG 
GAGCAAGCTC GGCCGAGCGC GGCGGCCATG CGCGCCGCCG CCGAGGCCGT TGACGCCGTC 
GCGGAGCTCG TCAAGAGGGT GCCGCAGCAG CAAGCGACGC CGGATGCCGC GCGCGGGTTC 
GTGAGGGACC TGGGGCTGGG AGCCGAGAAG CTCTCGTTCA CCTTCCGGCC GCCGGAGGTG 
GTGCGGCTGG CCGGGAGCCA CGCGGCTGGC GCGGTCGCGA GGCCAGACGT CGCTGCTGAC 
CTTCTCGTGC GCTTGCCAAA GGAATGCTTC CACGAGAAGG ATTTCCTGAA TCACCGGTAC 
CATGCAAAGA GGTGTCTTTA CCTCTGTGTT GTCGAGAAAA ACTTGAGGTG CTCTAAATTA 
ATCCACAAAG TTTCATGGTC TACCCTCCAA GATGAGGCTC GAAAGCCTGT GCTTCATGTA 
TATCCAGCAA TCGAAATTGC AGACCTTCCT GGATTCTATG TAAGAATAAT CCCAACTGCG 
GATTCTTTGT TCAACGTTTC AAAGTTGAAT GTATCAACGA GAAACAATGT CCGGGCATAT 
ACAAAAGATG GTGTAAACCT ACCCACACCA AAGTACAATT GTAGCATATT GGAAGATATG 
TTTTTGGAGG AAAATGCAGA CTTTATTAGT AGCACCTTTG CTAACTGGAA AGCTTTGCAG 
GAGGCCTTGG TGTTGGTAAA GGTTTGGGCA CGTCAAAGAA CTTCAATATA CACACATGAT 
TGTCTCAATG GATACTTAAT TTCTGCCATC CTTGTGTTTC TTACTGTGGA CTCTGGAGGA 
AGCATGATTA CTAGATCAAT GACCACAAGA CAAATTTTTC GTGTATTGAT GAATTTTTTG 
GCAACTTCCA AGGTGTGGGC AAAGGGATTG GTGATTCAAT CAATGAAGAA GCGTACAATC 
ACCAAAGAGG ACATTGCCAC TTGCCTGAAG ACATTTGATG TTACCGTATT TGATATATCT 
GGCCACGTAA ATTTGGCGTT TCGGATGACG AAATCGGCTT TTTTAGAGCT TCAAGATGAG 
GCAGCTTGCG CGCTTAGTTG CCTTGATAAA TGCAGAGATG GTGGACTTGA AGAGCTTTTT 
ATGACCAAAG TTGACTTTTG TGCTAAGTTT GACTCATGCT TGAGGATAAA CTTGAAAGGG 
AACTCTAAGG TTACTGAGTT AAACTATTGT GTGGATGACG AGTCTTGGAG AATTCTTGAG 
AAAGATGTTC AGTCTTTGCT GCAACGAGGA CTTACAGATA GAACAAAGAT GATCCGAGTC 
CTGTGGAGAA GCACACCTTC TGAATGGAAA ATAGTGGAAG GTTTCTCAGA ATTTGGCAGC 
AGTCCGCTTC TCGTTGGTAT GATGGTTAGC TCATTGGAAA AGAGTTTTCG CCTTGTTGAT 
ATTGGTCCAA ACCCTGAGAA CCGCGTTGAG GCTATTAAAT TTAGGAAATT TTGGGGAGAG 
AAAGCTGAGC TTAGAAGATT CAAGGATGGA AATATTGCTG AAAGCACAGT TTGGGAATGC 
CAATCTTGGG AGAAGCACAC AATTATTAAA AGGATAGCTG ATTATGTGCT TATGAAACAT 
CTGTCACTGC AGAAGGATGA TTTGATTCAT GTTGTTGATC AGCTGGACTT TTGCCTCTTA 
GTTGATGGCC AAGATCCGGT CTCGTCTTCT GGAGCTTTGC TTGAAGCTTT TGATACTATT 
TCTAAGCAGT TACGTATCTT AGATGATATT CCTCTTAAAA TATCTACCGT GCAACCCCTA 
GACTCAGCTT TTAGGCACAC TTCTGTGTTT CCTCCTGAAC CTCATCCACT AGCATATGGA 
AGGAATTCTC AGAGGCTACC GAAATTCGCA ACAACTTGCA TAAGATCTTT AGAAGTTATG 
ATTCAGCTAG AGGGATCTGG TAACTGGCCC TTGGATCCTG TGGCCATGGA GAAGACAAAG 
GCTGCATTTC TTTTAAAAAT TGGTGAAAGC TTGGAGGATC GAGGAATGTT TGTTTCAGCC 
AGTGAAGATG AGGTGAATGT CCTTACATCT GGATATTCAT TTTTACTCAA AATATTTCAT 
GAAAGAGGTT TAGCGCTGCA AAAGCCAGTT GGAGATGACA AGACACAAAG TGCTCTATCA 
GAAGATAAGA TGCTTTTTCA GCGGAGCCAA CACTCTAGTA TGATTAATGG TCTGCATGGC 
CGTTACCAGA TGTATGGACC AGTAGTTAGG CTAGCAAAAA GATGGATTTC TGCACATCTA 
TTTTCTTCAT TTATCTCAGA GGAGGCAGTT GAGTTGGTAG TTGCGTACAT ATTTTTGAAG 
CCATTCCCGT TCCATGCCCC TTCTTCTCGG GTTGCTGGGT TTCTCAGGTT TTTGCGGTTG 
TTATCAAGTT TTGACTGGAC CTTTTCACCC ATGGTTATTG ACATAAATAA TGACTTCAAC 
CTGAAGGATG AGAAGGAAAT CAATGATAAC TTTATGCTGA GCAGAAAATC TTATGAACAA 
AGCCCTCATG ATATAGAGCC TGCAATGTTT CTCGCTACGT CATATGATAA AGCATCTGAA 
GCATGGACAA AACAGTCACC AAGCAAGTCG GTCCTTAAAC GGATGGCTGC TTATGCTAAA 
AGCAGTGCAC AGCTTTTAAC AAATCTTATA CTTCATGGTC AATCTGGAGA ATATACATGG 
GAGTGCCTGT TCCGAACACC TATGAGCAAT TATGATGCTG TTATTCTCCT CCATCAAGAG 
AAGCTTTGTT GTCCCCATCA TGTACTTTTT CCTGCCGAAA CTCCAGAAGG TAAGTTGGTG 
GTCTGGGGCA AACCAAGTAA AGATTTCTGC CCCTATATGC CACTCAACAA AGGGGCAGTG 
AAAGGTTTTC ATGACGCAAG AGATAAGCTT TTGGTGAACT TTGACCCAAC TACATACTTC 
TTGCGAGACT TGAAGTGTGA ATTCTCCAAG ACTTTCAAGT TGTGGTATGG TTCAATTGGA 
GGAGACGCTG TGGGACTTAC TTGGGAGAAC CCAAAGAAGC GTGGCAGAGA GGAGGCTGAT 
GAAACTGAGC CAGAGCCCAC ATCTATCCTT AAGGAGGTTG GAGACGTTGG TAAAGGTTTA 
GTGAGGGGTG TGTATCTTGT CAAGGCACCA AAGCTTCAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021302077.1
CDS	81..3242
Translation	MATTSAASEYSVDRKLSALVEQARPSAAAMRAAAEAVDAVAELVKRVPQQQATPDAARGFVRDLGLGAEKLSFTFRPPEVVRLAGSHAAGAVARPDVAADLLVRLPKECFHEKDFLNHRYHAKRCLYLCVVEKNLRCSKLIHKVSWSTLQDEARKPVLHVYPAIEIADLPGFYVRIIPTADSLFNVSKLNVSTRNNVRAYTKDGVNLPTPKYNCSILEDMFLEENADFISSTFANWKALQEALVLVKVWARQRTSIYTHDCLNGYLISAILVFLTVDSGGSMITRSMTTRQIFRVLMNFLATSKVWAKGLVIQSMKKRTITKEDIATCLKTFDVTVFDISGHVNLAFRMTKSAFLELQDEAACALSCLDKCRDGGLEELFMTKVDFCAKFDSCLRINLKGNSKVTELNYCVDDESWRILEKDVQSLLQRGLTDRTKMIRVLWRSTPSEWKIVEGFSEFGSSPLLVGMMVSSLEKSFRLVDIGPNPENRVEAIKFRKFWGEKAELRRFKDGNIAESTVWECQSWEKHTIIKRIADYVLMKHLSLQKDDLIHVVDQLDFCLLVDGQDPVSSSGALLEAFDTISKQLRILDDIPLKISTVQPLDSAFRHTSVFPPEPHPLAYGRNSQRLPKFATTCIRSLEVMIQLEGSGNWPLDPVAMEKTKAAFLLKIGESLEDRGMFVSASEDEVNVLTSGYSFLLKIFHERGLALQKPVGDDKTQSALSEDKMLFQRSQHSSMINGLHGRYQMYGPVVRLAKRWISAHLFSSFISEEAVELVVAYIFLKPFPFHAPSSRVAGFLRFLRLLSSFDWTFSPMVIDINNDFNLKDEKEINDNFMLSRKSYEQSPHDIEPAMFLATSYDKASEAWTKQSPSKSVLKRMAAYAKSSAQLLTNLILHGQSGEYTWECLFRTPMSNYDAVILLHQEKLCCPHHVLFPAETPEGKLVVWGKPSKDFCPYMPLNKGAVKGFHDARDKLLVNFDPTTYFLRDLKCEFSKTFKLWYGSIGGDAVGLTWENPKKRGREEADETEPEPTSILKEVGDVGKGLVRGVYLVKAPKLQ

Target ORF information:

RefSeq Version	XM_021446402.1
Organism	Sorghum bicolor(sorghum)
Definition	Sorghum bicolor nucleolar protein 6 (LOC8073495), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021446402.1

ORF Insert Sequence:

ATGGCCACCA CCTCCGCCGC CTCCGAGTAC TCCGTCGACC GCAAGCTCTC CGCGCTCGTG 
GAGCAAGCTC GGCCGAGCGC GGCGGCCATG CGCGCCGCCG CCGAGGCCGT TGACGCCGTC 
GCGGAGCTCG TCAAGAGGGT GCCGCAGCAG CAAGCGACGC CGGATGCCGC GCGCGGGTTC 
GTGAGGGACC TGGGGCTGGG AGCCGAGAAG CTCTCGTTCA CCTTCCGGCC GCCGGAGGTG 
GTGCGGCTGG CCGGGAGCCA CGCGGCTGGC GCGGTCGCGA GGCCAGACGT CGCTGCTGAC 
CTTCTCGTGC GCTTGCCAAA GGAATGCTTC CACGAGAAGG ATTTCCTGAA TCACCGGTAC 
CATGCAAAGA GGTGTCTTTA CCTCTGTGTT GTCGAGAAAA ACTTGAGGTG CTCTAAATTA 
ATCCACAAAG TTTCATGGTC TACCCTCCAA GATGAGGCTC GAAAGCCTGT GCTTCATGTA 
TATCCAGCAA TCGAAATTGC AGACCTTCCT GGATTCTATG TAAGAATAAT CCCAACTGCG 
GATTCTTTGT TCAACGTTTC AAAGTTGAAT GTATCAACGA GAAACAATGT CCGGGCATAT 
ACAAAAGATG GTGTAAACCT ACCCACACCA AAGTACAATT GTAGCATATT GGAAGATATG 
TTTTTGGAGG AAAATGCAGA CTTTATTAGT AGCACCTTTG CTAACTGGAA AGCTTTGCAG 
GAGGCCTTGG TGTTGGTAAA GGTTTGGGCA CGTCAAAGAA CTTCAATATA CACACATGAT 
TGTCTCAATG GATACTTAAT TTCTGCCATC CTTGTGTTTC TTACTGTGGA CTCTGGAGGA 
AGCATGATTA CTAGATCAAT GACCACAAGA CAAATTTTTC GTGTATTGAT GAATTTTTTG 
GCAACTTCCA AGGTGTGGGC AAAGGGATTG GTGATTCAAT CAATGAAGAA GCGTACAATC 
ACCAAAGAGG ACATTGCCAC TTGCCTGAAG ACATTTGATG TTACCGTATT TGATATATCT 
GGCCACGTAA ATTTGGCGTT TCGGATGACG AAATCGGCTT TTTTAGAGCT TCAAGATGAG 
GCAGCTTGCG CGCTTAGTTG CCTTGATAAA TGCAGAGATG GTGGACTTGA AGAGCTTTTT 
ATGACCAAAG TTGACTTTTG TGCTAAGTTT GACTCATGCT TGAGGATAAA CTTGAAAGGG 
AACTCTAAGG TTACTGAGTT AAACTATTGT GTGGATGACG AGTCTTGGAG AATTCTTGAG 
AAAGATGTTC AGTCTTTGCT GCAACGAGGA CTTACAGATA GAACAAAGAT GATCCGAGTC 
CTGTGGAGAA GCACACCTTC TGAATGGAAA ATAGTGGAAG GTTTCTCAGA ATTTGGCAGC 
AGTCCGCTTC TCGTTGGTAT GATGGTTAGC TCATTGGAAA AGAGTTTTCG CCTTGTTGAT 
ATTGGTCCAA ACCCTGAGAA CCGCGTTGAG GCTATTAAAT TTAGGAAATT TTGGGGAGAG 
AAAGCTGAGC TTAGAAGATT CAAGGATGGA AATATTGCTG AAAGCACAGT TTGGGAATGC 
CAATCTTGGG AGAAGCACAC AATTATTAAA AGGATAGCTG ATTATGTGCT TATGAAACAT 
CTGTCACTGC AGAAGGATGA TTTGATTCAT GTTGTTGATC AGCTGGACTT TTGCCTCTTA 
GTTGATGGCC AAGATCCGGT CTCGTCTTCT GGAGCTTTGC TTGAAGCTTT TGATACTATT 
TCTAAGCAGT TACGTATCTT AGATGATATT CCTCTTAAAA TATCTACCGT GCAACCCCTA 
GACTCAGCTT TTAGGCACAC TTCTGTGTTT CCTCCTGAAC CTCATCCACT AGCATATGGA 
AGGAATTCTC AGAGGCTACC GAAATTCGCA ACAACTTGCA TAAGATCTTT AGAAGTTATG 
ATTCAGCTAG AGGGATCTGG TAACTGGCCC TTGGATCCTG TGGCCATGGA GAAGACAAAG 
GCTGCATTTC TTTTAAAAAT TGGTGAAAGC TTGGAGGATC GAGGAATGTT TGTTTCAGCC 
AGTGAAGATG AGGTGAATGT CCTTACATCT GGATATTCAT TTTTACTCAA AATATTTCAT 
GAAAGAGGTT TAGCGCTGCA AAAGCCAGTT GGAGATGACA AGACACAAAG TGCTCTATCA 
GAAGATAAGA TGCTTTTTCA GCGGAGCCAA CACTCTAGTA TGATTAATGG TCTGCATGGC 
CGTTACCAGA TGTATGGACC AGTAGTTAGG CTAGCAAAAA GATGGATTTC TGCACATCTA 
TTTTCTTCAT TTATCTCAGA GGAGGCAGTT GAGTTGGTAG TTGCGTACAT ATTTTTGAAG 
CCATTCCCGT TCCATGCCCC TTCTTCTCGG GTTGCTGGGT TTCTCAGGTT TTTGCGGTTG 
TTATCAAGTT TTGACTGGAC CTTTTCACCC ATGGTTATTG ACATAAATAA TGACTTCAAC 
CTGAAGGATG AGAAGGAAAT CAATGATAAC TTTATGCTGA GCAGAAAATC TTATGAACAA 
AGCCCTCATG ATATAGAGCC TGCAATGTTT CTCGCTACGT CATATGATAA AGCATCTGAA 
GCATGGACAA AACAGTCACC AAGCAAGTCG GTCCTTAAAC GGATGGCTGC TTATGCTAAA 
AGCAGTGCAC AGCTTTTAAC AAATCTTATA CTTCATGGTC AATCTGGAGA ATATACATGG 
GAGTGCCTGT TCCGAACACC TATGAGCAAT TATGATGCTG TTATTCTCCT CCATCAAGAG 
AAGCTTTGTT GTCCCCATCA TGTACTTTTT CCTGCCGAAA CTCCAGAAGG TAAGTTGGTG 
GTCTGGGGCA AACCAAGTAA AGATTTCTGC CCCTATATGC CACTCAACAA AGGGGCAGTG 
AAAGGTTTTC ATGACGCAAG AGATAAGCTT TTGGTGAACT TTGACCCAAC TACATACTTC 
TTGCGAGACT TGAAGTGTGA ATTCTCCAAG ACTTTCAAGT TGTGGTATGG TTCAATTGGA 
GGAGACGCTG TGGGACTTAC TTGGGAGAAC CCAAAGAAGC GTGGCAGAGA GGAGGCTGAT 
GAAACTGAGC CAGAGCCCAC ATCTATCCTT AAGGAGGTTG GAGACGTTGG TAAAGGTTTA 
GTGAGGGGTG TGTATCTTGT CAAGGCACCA AAGCTTCAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC8073495 ( XM_002441835.1 ) cDNA ORF clone, Sorghum bicolor(sorghum) -> XP_002441880.1 Sorghum bicolor hypothetical protein, mRNA.

Price & Availability↑

CloneID	OSc12027
Clone ID Related Accession (Same CDS sequence)	XM_002441835.1
Accession Version	XM_002441835.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3027bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1247328000000
Organism	Sorghum bicolor(Sorghum)
Product	hypothetical protein
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_012877). COMPLETENESS: incomplete on the 5' end.

ATGGCCACCA CCTCCGCCGC CTCCGAGTAC TCCGTCGACC GCAAGCTCTC CGCGCTCGTG 
GAGCAAGCTC GGCCGAGCGC GGCGGCCATG CGCGCCGCCG CCGAGGCCGT TGACGCCGTC 
GCGGAGCTCG TCAAGAGGGT GCCGCAGCAG CAAGCGACGC CGGATGCCGC GCGCGGGTTC 
GTGAGGGACC TGGGGCTGGG AGCCGAGAAG CTCTCGTTCA CCTTCCGGCC GCCGGAGGTG 
GTGCGGCTGG CCGGGAGCCA CGCGGCTGGC GCGGTCGCGA GGCCAGACGT CGCTGCTGAC 
CTTCTCGTGC GCTTGCCAAA GGAATGCTTC CACGAGAAGG ATTTCCTGAA TCACCGGTAC 
CATGCAAAGA GGTGTCTTTA CCTCTGTGTT GTCGAGAAAA ACTTGAGGTG CTCTAAATTA 
ATCCACAAAG TTTCATGGTC TACCCTCCAA GATGAGGCTC GAAAGCCTGT GCTTCATGTA 
TATCCAGCAA TCGAAATTGC AGACCTTCCT GGATTCTATG TAAGAATAAT CCCAACTGCG 
GATTCTTTGT TCAACGTTTC AAAGTTGAAT GTATCAACGA GAAACAATGT CCGGGCATAT 
ACAAAAGATG GTGTAAACCT ACCCACACCA AAGTACAATT GTAGCATATT GGAAGATATG 
TTTTTGGAGG AAAATGCAGA CTTTATTAGT AGCACCTTTG CTAACTGGAA AGCTTTGCAG 
GAGGCCTTGG TGTTGGTAAA GTTGAAGCAT ATCTGTATTC TTGTAGCAAC TTCCAAGGTG 
TGGGCAAAGG GATTGGTGAT TCAATCAATG AAGAAGCGTA CAATCACCAA AGAGGACATT 
GCCACTTGCC TGAAGACATT TGATGTTACC GTATTTGATA TATCTGGCCA CGTAAATTTG 
GCGTTTCGGA TGACGAAATC GGCTTTTTTA GAGCTTCAAG ATGAGGCAGC TTGCGCGCTT 
AGTTGCCTTG ATAAATGCAG AGATGGTGGA CTTGAAGAGC TTTTTATGAC CAAAGTTGAC 
TTTTGTGCTA AGTTTGACTC ATGCTTGAGG ATAAACTTGA AAGGGAACTC TAAGGTTACT 
GAGTTAAACT ATTGTGTGGA TGACGAGTCT TGGAGAATTC TTGAGAAAGA TGTTCAGTCT 
TTGCTGCAAC GAGGACTTAC AGATAGAACA AAGATGATCC GAGTCCTGTG GAGAAGCACA 
CCTTCTGAAT GGAAAATAGT GGAAGGTTTC TCAGAATTTG GCAGCAGTCC GCTTCTCGTT 
GGTATGATGG TTAGCTCATT GGAAAAGAGT TTTCGCCTTG TTGATATTGG TCCAAACCCT 
GAGAACCGCG TTGAGGCTAT TAAATTTAGG AAATTTTGGG GAGAGAAAGC TGAGCTTAGA 
AGATTCAAGG ATGGAAATAT TGCTGAAAGC ACAGTTTGGG AATGCCAATC TTGGGAGAAG 
CACACAATTA TTAAAAGGAT AGCTGATTAT GTGCTTATGA AACATCTGTC ACTGCAGAAG 
GATGATTTGA TTCATGTTGT TGATCAGCTG GACTTTTGCC TCTTAGTTGA TGGCCAAGAT 
CCGGTCTCGT CTTCTGGAGC TTTGCTTGAA GCTTTTGATA CTATTTCTAA GCAGTTACGT 
ATCTTAGATG ATATTCCTCT TAAAATATCT ACCGTGCAAC CCCTAGACTC AGCTTTTAGG 
CACACTTCTG TGTTTCCTCC TGAACCTCAT CCACTAGCAT ATGGAAGGAA TTCTCAGAGG 
CTACCGAAAT TCGCAACAAC TTGCATAAGA TCTTTAGAAG TTATGATTCA GCTAGAGGGA 
TCTGGTAACT GGCCCTTGGA TCCTGTGGCC ATGGAGAAGA CAAAGGCTGC ATTTCTTTTA 
AAAATTGGTG AAAGCTTGGA GGATCGAGGA ATGTTTGTTT CAGCCAGTGA AGATGAGGTG 
AATGTCCTTA CATCTGGATA TTCATTTTTA CTCAAAATAT TTCATGAAAG AGGTTTAGCG 
CTGCAAAAGC CAGTTGGAGA TGACAAGACA CAAAGTGCTC TATCAGAAGA TAAGATGCTT 
TTTCAGCGGA GCCAACACTC TAGTATGATT AATGGTCTGC ATGGCCGTTA CCAGATGTAT 
GGACCAGTAG TTAGGCTAGC AAAAAGATGG ATTTCTGCAC ATCTATTTTC TTCATTTATC 
TCAGAGGAGG CAGTTGAGTT GGTAGTTGCG TACATATTTT TGAAGCCATT CCCGTTCCAT 
GCCCCTTCTT CTCGGGTTGC TGGGTTTCTC AGGTTTTTGC GGTTGTTATC AAGTTTTGAC 
TGGACCTTTT CACCCATGGT TATTGACATA AATAATGACT TCAACCTGAA GGATGAGAAG 
GAAATCAATG ATAACTTTAT GCTGAGCAGA AAATCTTATG AACAAAGCCC TCATGATATA 
GAGCCTGCAA TGTTTCTCGC TACGTCATAT GATAAAGCAT CTGAAGCATG GACAAAACAG 
TCACCAAGCA AGTCGGTCCT TAAACGGATG GCTGCTTATG CTAAAAGCAG TGCACAGCTT 
TTAACAAATC TTATACTTCA TGGTCAATCT GGAGAATATA CATGGGAGTG CCTGTTCCGA 
ACACCTATGA GCAATTATGA TGCTGTTATT CTCCTCCATC AAGAGAAGCT TTGTTGTCCC 
CATCATGTAC TTTTTCCTGC CGAAACTCCA GAAGGTAAGT TGGTGGTCTG GGGCAAACCA 
AGTAAAGATT TCTGCCCCTA TATGCCACTC AACAAAGGGG CAGTGAAAGG TTTTCATGAC 
GCAAGAGATA AGCTTTTGGT GAACTTTGAC CCAACTACAT ACTTCTTGCG AGACTTGAAG 
TGTGAATTCT CCAAGACTTT CAAGTTGTGG TATGGTTCAA TTGGAGGAGA CGCTGTGGGA 
CTTACTTGGG AGAACCCAAA GAAGCGTGGC AGAGAGGAGG CTGATGAAAC TGAGCCAGAG 
CCCACATCTA TCCTTAAGGA GGTTGGAGAC GTTGGTAAAG GTTTAGTGAG GGGTGTGTAT 
CTTGTCAAGG CACCAAAGCT TCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002441880.1
CDS	1..3027
Translation	MATTSAASEYSVDRKLSALVEQARPSAAAMRAAAEAVDAVAELVKRVPQQQATPDAARGFVRDLGLGAEKLSFTFRPPEVVRLAGSHAAGAVARPDVAADLLVRLPKECFHEKDFLNHRYHAKRCLYLCVVEKNLRCSKLIHKVSWSTLQDEARKPVLHVYPAIEIADLPGFYVRIIPTADSLFNVSKLNVSTRNNVRAYTKDGVNLPTPKYNCSILEDMFLEENADFISSTFANWKALQEALVLVKLKHICILVATSKVWAKGLVIQSMKKRTITKEDIATCLKTFDVTVFDISGHVNLAFRMTKSAFLELQDEAACALSCLDKCRDGGLEELFMTKVDFCAKFDSCLRINLKGNSKVTELNYCVDDESWRILEKDVQSLLQRGLTDRTKMIRVLWRSTPSEWKIVEGFSEFGSSPLLVGMMVSSLEKSFRLVDIGPNPENRVEAIKFRKFWGEKAELRRFKDGNIAESTVWECQSWEKHTIIKRIADYVLMKHLSLQKDDLIHVVDQLDFCLLVDGQDPVSSSGALLEAFDTISKQLRILDDIPLKISTVQPLDSAFRHTSVFPPEPHPLAYGRNSQRLPKFATTCIRSLEVMIQLEGSGNWPLDPVAMEKTKAAFLLKIGESLEDRGMFVSASEDEVNVLTSGYSFLLKIFHERGLALQKPVGDDKTQSALSEDKMLFQRSQHSSMINGLHGRYQMYGPVVRLAKRWISAHLFSSFISEEAVELVVAYIFLKPFPFHAPSSRVAGFLRFLRLLSSFDWTFSPMVIDINNDFNLKDEKEINDNFMLSRKSYEQSPHDIEPAMFLATSYDKASEAWTKQSPSKSVLKRMAAYAKSSAQLLTNLILHGQSGEYTWECLFRTPMSNYDAVILLHQEKLCCPHHVLFPAETPEGKLVVWGKPSKDFCPYMPLNKGAVKGFHDARDKLLVNFDPTTYFLRDLKCEFSKTFKLWYGSIGGDAVGLTWENPKKRGREEADETEPEPTSILKEVGDVGKGLVRGVYLVKAPKLQ

Target ORF information:

RefSeq Version	XM_002441835.1
Organism	Sorghum bicolor(Sorghum)
Definition	Sorghum bicolor hypothetical protein, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002441835.1

ORF Insert Sequence:

ATGGCCACCA CCTCCGCCGC CTCCGAGTAC TCCGTCGACC GCAAGCTCTC CGCGCTCGTG 
GAGCAAGCTC GGCCGAGCGC GGCGGCCATG CGCGCCGCCG CCGAGGCCGT TGACGCCGTC 
GCGGAGCTCG TCAAGAGGGT GCCGCAGCAG CAAGCGACGC CGGATGCCGC GCGCGGGTTC 
GTGAGGGACC TGGGGCTGGG AGCCGAGAAG CTCTCGTTCA CCTTCCGGCC GCCGGAGGTG 
GTGCGGCTGG CCGGGAGCCA CGCGGCTGGC GCGGTCGCGA GGCCAGACGT CGCTGCTGAC 
CTTCTCGTGC GCTTGCCAAA GGAATGCTTC CACGAGAAGG ATTTCCTGAA TCACCGGTAC 
CATGCAAAGA GGTGTCTTTA CCTCTGTGTT GTCGAGAAAA ACTTGAGGTG CTCTAAATTA 
ATCCACAAAG TTTCATGGTC TACCCTCCAA GATGAGGCTC GAAAGCCTGT GCTTCATGTA 
TATCCAGCAA TCGAAATTGC AGACCTTCCT GGATTCTATG TAAGAATAAT CCCAACTGCG 
GATTCTTTGT TCAACGTTTC AAAGTTGAAT GTATCAACGA GAAACAATGT CCGGGCATAT 
ACAAAAGATG GTGTAAACCT ACCCACACCA AAGTACAATT GTAGCATATT GGAAGATATG 
TTTTTGGAGG AAAATGCAGA CTTTATTAGT AGCACCTTTG CTAACTGGAA AGCTTTGCAG 
GAGGCCTTGG TGTTGGTAAA GTTGAAGCAT ATCTGTATTC TTGTAGCAAC TTCCAAGGTG 
TGGGCAAAGG GATTGGTGAT TCAATCAATG AAGAAGCGTA CAATCACCAA AGAGGACATT 
GCCACTTGCC TGAAGACATT TGATGTTACC GTATTTGATA TATCTGGCCA CGTAAATTTG 
GCGTTTCGGA TGACGAAATC GGCTTTTTTA GAGCTTCAAG ATGAGGCAGC TTGCGCGCTT 
AGTTGCCTTG ATAAATGCAG AGATGGTGGA CTTGAAGAGC TTTTTATGAC CAAAGTTGAC 
TTTTGTGCTA AGTTTGACTC ATGCTTGAGG ATAAACTTGA AAGGGAACTC TAAGGTTACT 
GAGTTAAACT ATTGTGTGGA TGACGAGTCT TGGAGAATTC TTGAGAAAGA TGTTCAGTCT 
TTGCTGCAAC GAGGACTTAC AGATAGAACA AAGATGATCC GAGTCCTGTG GAGAAGCACA 
CCTTCTGAAT GGAAAATAGT GGAAGGTTTC TCAGAATTTG GCAGCAGTCC GCTTCTCGTT 
GGTATGATGG TTAGCTCATT GGAAAAGAGT TTTCGCCTTG TTGATATTGG TCCAAACCCT 
GAGAACCGCG TTGAGGCTAT TAAATTTAGG AAATTTTGGG GAGAGAAAGC TGAGCTTAGA 
AGATTCAAGG ATGGAAATAT TGCTGAAAGC ACAGTTTGGG AATGCCAATC TTGGGAGAAG 
CACACAATTA TTAAAAGGAT AGCTGATTAT GTGCTTATGA AACATCTGTC ACTGCAGAAG 
GATGATTTGA TTCATGTTGT TGATCAGCTG GACTTTTGCC TCTTAGTTGA TGGCCAAGAT 
CCGGTCTCGT CTTCTGGAGC TTTGCTTGAA GCTTTTGATA CTATTTCTAA GCAGTTACGT 
ATCTTAGATG ATATTCCTCT TAAAATATCT ACCGTGCAAC CCCTAGACTC AGCTTTTAGG 
CACACTTCTG TGTTTCCTCC TGAACCTCAT CCACTAGCAT ATGGAAGGAA TTCTCAGAGG 
CTACCGAAAT TCGCAACAAC TTGCATAAGA TCTTTAGAAG TTATGATTCA GCTAGAGGGA 
TCTGGTAACT GGCCCTTGGA TCCTGTGGCC ATGGAGAAGA CAAAGGCTGC ATTTCTTTTA 
AAAATTGGTG AAAGCTTGGA GGATCGAGGA ATGTTTGTTT CAGCCAGTGA AGATGAGGTG 
AATGTCCTTA CATCTGGATA TTCATTTTTA CTCAAAATAT TTCATGAAAG AGGTTTAGCG 
CTGCAAAAGC CAGTTGGAGA TGACAAGACA CAAAGTGCTC TATCAGAAGA TAAGATGCTT 
TTTCAGCGGA GCCAACACTC TAGTATGATT AATGGTCTGC ATGGCCGTTA CCAGATGTAT 
GGACCAGTAG TTAGGCTAGC AAAAAGATGG ATTTCTGCAC ATCTATTTTC TTCATTTATC 
TCAGAGGAGG CAGTTGAGTT GGTAGTTGCG TACATATTTT TGAAGCCATT CCCGTTCCAT 
GCCCCTTCTT CTCGGGTTGC TGGGTTTCTC AGGTTTTTGC GGTTGTTATC AAGTTTTGAC 
TGGACCTTTT CACCCATGGT TATTGACATA AATAATGACT TCAACCTGAA GGATGAGAAG 
GAAATCAATG ATAACTTTAT GCTGAGCAGA AAATCTTATG AACAAAGCCC TCATGATATA 
GAGCCTGCAA TGTTTCTCGC TACGTCATAT GATAAAGCAT CTGAAGCATG GACAAAACAG 
TCACCAAGCA AGTCGGTCCT TAAACGGATG GCTGCTTATG CTAAAAGCAG TGCACAGCTT 
TTAACAAATC TTATACTTCA TGGTCAATCT GGAGAATATA CATGGGAGTG CCTGTTCCGA 
ACACCTATGA GCAATTATGA TGCTGTTATT CTCCTCCATC AAGAGAAGCT TTGTTGTCCC 
CATCATGTAC TTTTTCCTGC CGAAACTCCA GAAGGTAAGT TGGTGGTCTG GGGCAAACCA 
AGTAAAGATT TCTGCCCCTA TATGCCACTC AACAAAGGGG CAGTGAAAGG TTTTCATGAC 
GCAAGAGATA AGCTTTTGGT GAACTTTGAC CCAACTACAT ACTTCTTGCG AGACTTGAAG 
TGTGAATTCT CCAAGACTTT CAAGTTGTGG TATGGTTCAA TTGGAGGAGA CGCTGTGGGA 
CTTACTTGGG AGAACCCAAA GAAGCGTGGC AGAGAGGAGG CTGATGAAAC TGAGCCAGAG 
CCCACATCTA TCCTTAAGGA GGTTGGAGAC GTTGGTAAAG GTTTAGTGAG GGGTGTGTAT 
CTTGTCAAGG CACCAAAGCT TCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Sorghum bicolor genome and the diversification of grasses.
Nature457(7229)551-6(2009 Jan)
Paterson AH,Bowers JE,Bruggmann R,Dubchak I,Grimwood J,Gundlach H,Haberer G,Hellsten U,Mitros T,Poliakov A,Schmutz J,Spannagl M,Tang H,Wang X,Wicker T,Bharti AK,Chapman J,Feltus FA,Gowik U,Grigoriev IV,Lyons E,Maher CA,Martis M,Narechania A,Otillar RP,Penning BW,Salamov AA,Wang Y,Zhang L,Carpita NC,Freeling M,Gingle AR,Hash CT,Keller B,Klein P,Kresovich S,McCann MC,Ming R,Peterson DG,Mehboob-ur-Rahman,Ware D,Westhoff P,Mayer KF,Messing J,Rokhsar DS

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LOC8073495 cDNA ORF clone, Sorghum bicolor(sorghum)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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