SORBIDRAFT_05G002950 cDNA ORF clone, Sorghum bicolor(Sorghum)

Gene
Clones
gRNAs

Summary

Gene Symbol	SORBIDRAFT_05G002950
Entrez Gene ID	8069315
Full Name	putative disease resistance RPP13-like protein 3
Gene Type	protein-coding
Organism	Sorghum bicolor(Sorghum)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002448943.2	XP_002448988.2	putative disease resistance RPP13-like protein 3
XM_002448943.1	XP_002448988.1	hypothetical protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The Sorghum bicolor genome and the diversification of grasses.
Paterson AH, Bowers JE, Bruggmann R, Dubchak I, Grimwood J, Gundlach H, Haberer G, Hellsten U, Mitros T, Poliakov A, Schmutz J, Spannagl M, Tang H, Wang X, Wicker T, Bharti AK, Chapman J, Feltus FA, Gowik U, Grigoriev IV, Lyons E, Maher CA, Martis M, Narechania A, Otillar RP, Penning BW, Salamov AA, Wang Y, Zhang L, Carpita NC, Freeling M, Gingle AR, Hash CT, Keller B, Klein P, Kresovich S, McCann MC, Ming R, Peterson DG, Mehboob-ur-Rahman, Ware D, Westhoff P, Mayer KF, Messing J, Rokhsar DS
Nature457(7229)551-6(2009 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SORBIDRAFT_05G002950 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SORBIDRAFT_05G002950 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc17110	XM_002448943.1 Latest version!	Sorghum bicolor hypothetical protein, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OSc119438	XM_002448943.2 Latest version!	Sorghum bicolor putative disease resistance RPP13-like protein 3 (LOC8069315), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SORBIDRAFT_05G002950 ( XM_002448943.1 ) cDNA ORF clone, Sorghum bicolor(Sorghum) -> XP_002448988.1 Sorghum bicolor hypothetical protein, mRNA.

Price & Availability↑

CloneID	OSc17110
Clone ID Related Accession (Same CDS sequence)	XM_002448943.1
Accession Version	XM_002448943.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2781bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1247328000000
Organism	Sorghum bicolor(Sorghum)
Product	hypothetical protein
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_012874). COMPLETENESS: incomplete on both ends.

ATGGCGGAAG CGATCATCGG TCCCCTGGTG GTGAAGCTGC AGGAGCTCGC CGTCAGCGAG 
GCGCGGGCGA TGGTGGCGGT GAACGACGAC ATCCGGAGCC TCCGGGACAA GCTCATGTGG 
ATGCAGGCCT TCCTGCGTGA CGCCGACCCG CGGCGCCGCG TCGTCCCCGA CGAGGCCAGC 
CGGGTGTGGC TCCACCAGAC GCGCGACGCC GCCTTCGACG CCGAGGACGC CGTCGACCAG 
TATTTCCTCC TCGTCGACCT ATCCAGGTAC CCAAGCTGGA CACGCACCAT TATAAGGTTC 
ATTGCCGGAT TAACTACACA AATACGTGTG CGGCGCAATC TTTCTAGCAA GATCAATGCT 
ATCAACTCAA GACTTGAGAA TATAATTAAG AACAAGGAAG CATACAAGCA AATCGGGGAC 
ACCAACAAGA CAGTTGTTCC ATGGAGGGCT TCCACGGCCA TCTCTGTTGC TCCAACCAAA 
TTGGACAATT TGCTGCAGCC TCCTCTTGTT AGTCGTGAAG ACAAGCGCGG ACAGCTCATT 
TCTGCTCTGC TTGATAGCAC TCATCAAAAG GTTATCTCTG TGATAGGGCC AAGTGGAGTG 
GGCAAGTCAA CTCTAGTGAG AGATGTGTAT GAAACGTTGG AAATCAAGAA CCATTTTGTT 
GAGCAGGCTT TGGCGACTTT CCCACCTTAT TCCAGTGCTT CCGATATCCT GAAATTAATC 
CTTAGGGATC TCAAAGAAGA AGACTTCACA TTATCTAAGA TGGAGGTCAC CAAGGAGCTG 
CTTGATAAGA AATTAAAAGG GAAGCAGTAC TTGGTGGTGA TAGATGGGGA AGTGAGCTCC 
ACTGAATGGA AAAACATCCT AGGTGCCCTC CCTAATGTAG CAGGAAGTAA AGTTGTGAGA 
ATGTCAAAAG AGAACCTAGA AGATCCTCCA ACAAACTATG AACATGTTGT TATCTCCCTT 
AACCGTTTTG ATAAGATTGC TACAACAGAA CTGTTCCAGC AGAGAGTGTG CAAGAAAGAG 
AGCAATCCTG AGTACAACAA AGACATCGAA GATGGTGTTC GAAACAAGTA CCAGCAAGAT 
ATCTTTGACA CCACACAGGG GCTACCACTT GCAATTGTTC TTCTTTCAGG CCTTCTACGA 
ACAAAGGAGT TTCCAGTCGA GTGGGAAGCT GTGTTTGATC ATCTTAAATC CAAGCAATCA 
AAGAGGCTTG ACAGCATACT TTCCCTGTGC TTTGATGATC TTCCATATGA CATAAAATCT 
TGCTTCCTCT ACTTTGCTGC ATTGCCAACA AACATGCTGA TTGAAGCACA AGATTTGGTG 
TGCATGTGGA TGGCAGAGGG CTTCCTGAGG CCAAAGGAAG GGTTGACAAT GGAGAAGGTT 
GGTTACCGGT ACTTGAAGGA GTTGATTGCA AGGCATTTAA TCAACCTAGA GCCTATGGAT 
GAGAATACCC CCGAGGAGGA GCTTGTGACC ATCCAAAGCA AAGTCCACGC ATTTCTTCAG 
ATTGAGGCAC AAGAGGTAAA TTTTGTGGAG ATCCACAACA GTGATGACAT CCCACCTCTG 
TCAGCTGCTC GCCGCCTCAG CTTGCAAAAT CGCATGGAGA AGTATGCTGC ACTAGCCAAC 
CCTATGCCAA AGCTTCGATC CATCTTGTCA AATTTTGAGA AGGAAGAAAC AAGCAAGGAA 
GACGAAGTCG CCGACGATGA AGAGGAAGCC AAAGTGCAAC AATCTCCAAC CTGCCTACAT 
TGCTCACCAC ATGGAACCAC AACCAAGTGC AAGGAGGGCG ATGCCAAGTC TTATCTGCAG 
CAACTTCTGC AAACATCCAA GTTCCTACGT GTGATTAACC TGCAAGGCCT TGAAGTTGGT 
GACAAGTTGC CAGATGAAAT TGGTAATGTT GTGCACTTGC AGTACCTTGC TGTGACATCC 
TGTTCTTTGA AAGAGGTACC GCCATCAGTT GGAAGGCTCT CTAGTCTTCA GACACTTGAT 
GTCCGAGACA CAGAAGTTAA GGAGCTACCG GTGCCATTCT GGGAGATTAG AACCCTAAGG 
CATGTGTTTG GCCATCGCCT CATCTTGCCA AAACGGGTTG GCGTCTTGAA GAACCTACAG 
ACCCTTGATA CTGTGAAACC TGATGCTAAG TATGGTTGGG ATAGAAATAC CCTTTCAAAA 
ATGATCCAGC TCCGTTCCTT GTTTATTTGG GAACTCTCCA AAGGTCATGA GAAGGGGCTA 
GTTGCTGCTC TGAAAAAACT CAAGTACCTT GTCACACTGA CCATACACGG TGACAGTATT 
CCATCGGCTG TTTTCACTAC TAAAACCTCG CTTCAACGAC TTGAGGTGAT GGAACTGGAT 
GGGATGCTTA TTGACATGCC ACGTGTAGTG GACATGGACA TCAAATCATG CTTGCCCAAT 
CTACTTCTTT TGTCATTAGA GAACACAATG GTGTCACAGG ACTTCATCAA CAAGTTGGCT 
GAGTTGCCCT TCCTTGCTAG TCTCACTTTG GATGGTGGAT CATATAAGGA TGAACAACTT 
GTATTATCTG CTGGTGGATT TCATAGTTTG AGGAGGTTGA CAGTGGACTT AGAAGAATTG 
AAGAAATTGG AAATACATGA ATCAGCACTG CCCAAGCTTG CAGATTTGGA TATTTTGATC 
CACTCTAATG ATCTCAAAAT TGTGATACTG GGTAAGAACG ATGTCGTTGA GAAGCTCCTT 
CATGAGGATA AAATACTCTC CAAGAAAATC CAACGGACTA CGAGGAGTGA ACGAACTGCC 
CGAAGGATCG GAGTTGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002448988.1
CDS	1..2781
Translation	MAEAIIGPLVVKLQELAVSEARAMVAVNDDIRSLRDKLMWMQAFLRDADPRRRVVPDEASRVWLHQTRDAAFDAEDAVDQYFLLVDLSRYPSWTRTIIRFIAGLTTQIRVRRNLSSKINAINSRLENIIKNKEAYKQIGDTNKTVVPWRASTAISVAPTKLDNLLQPPLVSREDKRGQLISALLDSTHQKVISVIGPSGVGKSTLVRDVYETLEIKNHFVEQALATFPPYSSASDILKLILRDLKEEDFTLSKMEVTKELLDKKLKGKQYLVVIDGEVSSTEWKNILGALPNVAGSKVVRMSKENLEDPPTNYEHVVISLNRFDKIATTELFQQRVCKKESNPEYNKDIEDGVRNKYQQDIFDTTQGLPLAIVLLSGLLRTKEFPVEWEAVFDHLKSKQSKRLDSILSLCFDDLPYDIKSCFLYFAALPTNMLIEAQDLVCMWMAEGFLRPKEGLTMEKVGYRYLKELIARHLINLEPMDENTPEEELVTIQSKVHAFLQIEAQEVNFVEIHNSDDIPPLSAARRLSLQNRMEKYAALANPMPKLRSILSNFEKEETSKEDEVADDEEEAKVQQSPTCLHCSPHGTTTKCKEGDAKSYLQQLLQTSKFLRVINLQGLEVGDKLPDEIGNVVHLQYLAVTSCSLKEVPPSVGRLSSLQTLDVRDTEVKELPVPFWEIRTLRHVFGHRLILPKRVGVLKNLQTLDTVKPDAKYGWDRNTLSKMIQLRSLFIWELSKGHEKGLVAALKKLKYLVTLTIHGDSIPSAVFTTKTSLQRLEVMELDGMLIDMPRVVDMDIKSCLPNLLLLSLENTMVSQDFINKLAELPFLASLTLDGGSYKDEQLVLSAGGFHSLRRLTVDLEELKKLEIHESALPKLADLDILIHSNDLKIVILGKNDVVEKLLHEDKILSKKIQRTTRSERTARRIGVE

Target ORF information:

RefSeq Version	XM_002448943.1
Organism	Sorghum bicolor(Sorghum)
Definition	Sorghum bicolor hypothetical protein, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002448943.1

ORF Insert Sequence:

ATGGCGGAAG CGATCATCGG TCCCCTGGTG GTGAAGCTGC AGGAGCTCGC CGTCAGCGAG 
GCGCGGGCGA TGGTGGCGGT GAACGACGAC ATCCGGAGCC TCCGGGACAA GCTCATGTGG 
ATGCAGGCCT TCCTGCGTGA CGCCGACCCG CGGCGCCGCG TCGTCCCCGA CGAGGCCAGC 
CGGGTGTGGC TCCACCAGAC GCGCGACGCC GCCTTCGACG CCGAGGACGC CGTCGACCAG 
TATTTCCTCC TCGTCGACCT ATCCAGGTAC CCAAGCTGGA CACGCACCAT TATAAGGTTC 
ATTGCCGGAT TAACTACACA AATACGTGTG CGGCGCAATC TTTCTAGCAA GATCAATGCT 
ATCAACTCAA GACTTGAGAA TATAATTAAG AACAAGGAAG CATACAAGCA AATCGGGGAC 
ACCAACAAGA CAGTTGTTCC ATGGAGGGCT TCCACGGCCA TCTCTGTTGC TCCAACCAAA 
TTGGACAATT TGCTGCAGCC TCCTCTTGTT AGTCGTGAAG ACAAGCGCGG ACAGCTCATT 
TCTGCTCTGC TTGATAGCAC TCATCAAAAG GTTATCTCTG TGATAGGGCC AAGTGGAGTG 
GGCAAGTCAA CTCTAGTGAG AGATGTGTAT GAAACGTTGG AAATCAAGAA CCATTTTGTT 
GAGCAGGCTT TGGCGACTTT CCCACCTTAT TCCAGTGCTT CCGATATCCT GAAATTAATC 
CTTAGGGATC TCAAAGAAGA AGACTTCACA TTATCTAAGA TGGAGGTCAC CAAGGAGCTG 
CTTGATAAGA AATTAAAAGG GAAGCAGTAC TTGGTGGTGA TAGATGGGGA AGTGAGCTCC 
ACTGAATGGA AAAACATCCT AGGTGCCCTC CCTAATGTAG CAGGAAGTAA AGTTGTGAGA 
ATGTCAAAAG AGAACCTAGA AGATCCTCCA ACAAACTATG AACATGTTGT TATCTCCCTT 
AACCGTTTTG ATAAGATTGC TACAACAGAA CTGTTCCAGC AGAGAGTGTG CAAGAAAGAG 
AGCAATCCTG AGTACAACAA AGACATCGAA GATGGTGTTC GAAACAAGTA CCAGCAAGAT 
ATCTTTGACA CCACACAGGG GCTACCACTT GCAATTGTTC TTCTTTCAGG CCTTCTACGA 
ACAAAGGAGT TTCCAGTCGA GTGGGAAGCT GTGTTTGATC ATCTTAAATC CAAGCAATCA 
AAGAGGCTTG ACAGCATACT TTCCCTGTGC TTTGATGATC TTCCATATGA CATAAAATCT 
TGCTTCCTCT ACTTTGCTGC ATTGCCAACA AACATGCTGA TTGAAGCACA AGATTTGGTG 
TGCATGTGGA TGGCAGAGGG CTTCCTGAGG CCAAAGGAAG GGTTGACAAT GGAGAAGGTT 
GGTTACCGGT ACTTGAAGGA GTTGATTGCA AGGCATTTAA TCAACCTAGA GCCTATGGAT 
GAGAATACCC CCGAGGAGGA GCTTGTGACC ATCCAAAGCA AAGTCCACGC ATTTCTTCAG 
ATTGAGGCAC AAGAGGTAAA TTTTGTGGAG ATCCACAACA GTGATGACAT CCCACCTCTG 
TCAGCTGCTC GCCGCCTCAG CTTGCAAAAT CGCATGGAGA AGTATGCTGC ACTAGCCAAC 
CCTATGCCAA AGCTTCGATC CATCTTGTCA AATTTTGAGA AGGAAGAAAC AAGCAAGGAA 
GACGAAGTCG CCGACGATGA AGAGGAAGCC AAAGTGCAAC AATCTCCAAC CTGCCTACAT 
TGCTCACCAC ATGGAACCAC AACCAAGTGC AAGGAGGGCG ATGCCAAGTC TTATCTGCAG 
CAACTTCTGC AAACATCCAA GTTCCTACGT GTGATTAACC TGCAAGGCCT TGAAGTTGGT 
GACAAGTTGC CAGATGAAAT TGGTAATGTT GTGCACTTGC AGTACCTTGC TGTGACATCC 
TGTTCTTTGA AAGAGGTACC GCCATCAGTT GGAAGGCTCT CTAGTCTTCA GACACTTGAT 
GTCCGAGACA CAGAAGTTAA GGAGCTACCG GTGCCATTCT GGGAGATTAG AACCCTAAGG 
CATGTGTTTG GCCATCGCCT CATCTTGCCA AAACGGGTTG GCGTCTTGAA GAACCTACAG 
ACCCTTGATA CTGTGAAACC TGATGCTAAG TATGGTTGGG ATAGAAATAC CCTTTCAAAA 
ATGATCCAGC TCCGTTCCTT GTTTATTTGG GAACTCTCCA AAGGTCATGA GAAGGGGCTA 
GTTGCTGCTC TGAAAAAACT CAAGTACCTT GTCACACTGA CCATACACGG TGACAGTATT 
CCATCGGCTG TTTTCACTAC TAAAACCTCG CTTCAACGAC TTGAGGTGAT GGAACTGGAT 
GGGATGCTTA TTGACATGCC ACGTGTAGTG GACATGGACA TCAAATCATG CTTGCCCAAT 
CTACTTCTTT TGTCATTAGA GAACACAATG GTGTCACAGG ACTTCATCAA CAAGTTGGCT 
GAGTTGCCCT TCCTTGCTAG TCTCACTTTG GATGGTGGAT CATATAAGGA TGAACAACTT 
GTATTATCTG CTGGTGGATT TCATAGTTTG AGGAGGTTGA CAGTGGACTT AGAAGAATTG 
AAGAAATTGG AAATACATGA ATCAGCACTG CCCAAGCTTG CAGATTTGGA TATTTTGATC 
CACTCTAATG ATCTCAAAAT TGTGATACTG GGTAAGAACG ATGTCGTTGA GAAGCTCCTT 
CATGAGGATA AAATACTCTC CAAGAAAATC CAACGGACTA CGAGGAGTGA ACGAACTGCC 
CGAAGGATCG GAGTTGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SORBIDRAFT_05G002950 ( XM_002448943.2 ) cDNA ORF clone, Sorghum bicolor(Sorghum) -> XP_002448988.2 Sorghum bicolor putative disease resistance RPP13-like protein 3 (LOC8069315), mRNA.

Price & Availability↑

CloneID	OSc119438
Clone ID Related Accession (Same CDS sequence)	XM_002448943.2
Accession Version	XM_002448943.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3069bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497196800000
Organism	Sorghum bicolor(sorghum)
Product	putative disease resistance RPP13-like protein 3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_012874.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 13, 2017 this sequence version replaced XM_002448943.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sorghum bicolor Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGTCAG GCTGGCTTTT ACCTTTATAT GCCATGGCAG AATCTGTTCC TAACTTCCTA 
TACATCTCGC GGGGTAACCG ATCGATCACC GACCGCACGT ATTCTGAATT TCTGATTCCT 
CTTTTCCATC ACGTTATGTG CCATGCATGC TTAATTAGCT TCCAGATGCA AATTGCCAAC 
TCGATCAAAG GGGTCGTCTT TATTTATTTG GGTTGGCATC GATCATTGGC AATTTGCATC 
GTCGGCCGGT GGTGTGAGAC TGAGAAGCTG CTGCAGGCCG GTGGAAGCAT GGCGGAAGCG 
ATCATCGGTC CCCTGGTGGT GAAGCTGCAG GAGCTCGCCG TCAGCGAGGC GCGGGCGATG 
GTGGCGGTGA ACGACGACAT CCGGAGCCTC CGGGACAAGC TCATGTGGAT GCAGGCCTTC 
CTGCGTGACG CCGACCCGCG GCGCCGCGTC GTCCCCGACG AGGCCAGCCG GGTGTGGCTC 
CACCAGACGC GCGACGCCGC CTTCGACGCC GAGGACGCCG TCGACCAGTA TTTCCTCCTC 
GTCGACCTAT CCAGGTACCC AAGCTGGACA CGCACCATTA TAAGGTTCAT TGCCGGATTA 
ACTACACAAA TACGTGTGCG GCGCAATCTT TCTAGCAAGA TCAATGCTAT CAACTCAAGA 
CTTGAGAATA TAATTAAGAA CAAGGAAGCA TACAAGCAAA TCGGGGACAC CAACAAGACA 
GTTGTTCCAT GGAGGGCTTC CACGGCCATC TCTGTTGCTC CAACCAAATT GGACAATTTG 
CTGCAGCCTC CTCTTGTTAG TCGTGAAGAC AAGCGCGGAC AGCTCATTTC TGCTCTGCTT 
GATAGCACTC ATCAAAAGGT TATCTCTGTG ATAGGGCCAA GTGGAGTGGG CAAGTCAACT 
CTAGTGAGAG ATGTGTATGA AACGTTGGAA ATCAAGAACC ATTTTGTTGA GCAGGCTTTG 
GCGACTTTCC CACCTTATTC CAGTGCTTCC GATATCCTGA AATTAATCCT TAGGGATCTC 
AAAGAAGAAG ACTTCACATT ATCTAAGATG GAGGTCACCA AGGAGCTGCT TGATAAGAAA 
TTAAAAGGGA AGCAGTACTT GGTGGTGATA GATGGGGAAG TGAGCTCCAC TGAATGGAAA 
AACATCCTAG GTGCCCTCCC TAATGTAGCA GGAAGTAAAG TTGTGAGAAT GTCAAAAGAG 
AACCTAGAAG ATCCTCCAAC AAACTATGAA CATGTTGTTA TCTCCCTTAA CCGTTTTGAT 
AAGATTGCTA CAACAGAACT GTTCCAGCAG AGAGTGTGCA AGAAAGAGAG CAATCCTGAG 
TACAACAAAG ACATCGAAGA TGGTGTTCGA AACAAGTACC AGCAAGATAT CTTTGACACC 
ACACAGGGGC TACCACTTGC AATTGTTCTT CTTTCAGGCC TTCTACGAAC AAAGGAGTTT 
CCAGTCGAGT GGGAAGCTGT GTTTGATCAT CTTAAATCCA AGCAATCAAA GAGGCTTGAC 
AGCATACTTT CCCTGTGCTT TGATGATCTT CCATATGACA TAAAATCTTG CTTCCTCTAC 
TTTGCTGCAT TGCCAACAAA CATGCTGATT GAAGCACAAG ATTTGGTGTG CATGTGGATG 
GCAGAGGGCT TCCTGAGGCC AAAGGAAGGG TTGACAATGG AGAAGGTTGG TTACCGGTAC 
TTGAAGGAGT TGATTGCAAG GCATTTAATC AACCTAGAGC CTATGGATGA GAATACCCCC 
GAGGAGGAGC TTGTGACCAT CCAAAGCAAA GTCCACGCAT TTCTTCAGAT TGAGGCACAA 
GAGGTAAATT TTGTGGAGAT CCACAACAGT GATGACATCC CACCTCTGTC AGCTGCTCGC 
CGCCTCAGCT TGCAAAATCG CATGGAGAAG TATGCTGCAC TAGCCAACCC TATGCCAAAG 
CTTCGATCCA TCTTGTCAAA TTTTGAGAAG GAAGAAACAA GCAAGGAAGA CGAAGTCGCC 
GACGATGAAG AGGAAGCCAA AGTGCAACAA TCTCCAACCT GCCTACATTG CTCACCACAT 
GGAACCACAA CCAAGTGCAA GGAGGGCGAT GCCAAGTCTT ATCTGCAGCA ACTTCTGCAA 
ACATCCAAGT TCCTACGTGT GATTAACCTG CAAGGCCTTG AAGTTGGTGA CAAGTTGCCA 
GATGAAATTG GTAATGTTGT GCACTTGCAG TACCTTGCTG TGACATCCTG TTCTTTGAAA 
GAGGTACCGC CATCAGTTGG AAGGCTCTCT AGTCTTCAGA CACTTGATGT CCGAGACACA 
GAAGTTAAGG AGCTACCGGT GCCATTCTGG GAGATTAGAA CCCTAAGGCA TGTGTTTGGC 
CATCGCCTCA TCTTGCCAAA ACGGGTTGGC GTCTTGAAGA ACCTACAGAC CCTTGATACT 
GTGAAACCTG ATGCTAAGTA TGGTTGGGAT AGAAATACCC TTTCAAAAAT GATCCAGCTC 
CGTTCCTTGT TTATTTGGGA ACTCTCCAAA GGTCATGAGA AGGGGCTAGT TGCTGCTCTG 
AAAAAACTCA AGTACCTTGT CACACTGACC ATACACGGTG ACAGTATTCC ATCGGCTGTT 
TTCACTACTA AAACCTCGCT TCAACGACTT GAGGTGATGG AACTGGATGG GATGCTTATT 
GACATGCCAC GTGTAGTGGA CATGGACATC AAATCATGCT TGCCCAATCT ACTTCTTTTG 
TCATTAGAGA ACACAATGGT GTCACAGGAC TTCATCAACA AGTTGGCTGA GTTGCCCTTC 
CTTGCTAGTC TCACTTTGGA TGGTGGATCA TATAAGGATG AACAACTTGT ATTATCTGCT 
GGTGGATTTC ATAGTTTGAG GAGGTTGACA GTGGACTTAG AAGAATTGAA GAAATTGGAA 
ATACATGAAT CAGCACTGCC CAAGCTTGCA GATTTGGATA TTTTGATCCA CTCTAATGAT 
CTCAAAATTG TGATACTGGG TAAGAACGAT GTCGTTGAGA AGCTCCTTCA TGAGGATAAA 
ATACTCTCCA AGAAAATCCA ACGGACTACG AGGAGTGAAC GAACTGCCCG AAGGATCGGA 
GTTGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002448988.2
CDS	276..3344
Translation	MPSGWLLPLYAMAESVPNFLYISRGNRSITDRTYSEFLIPLFHHVMCHACLISFQMQIANSIKGVVFIYLGWHRSLAICIVGRWCETEKLLQAGGSMAEAIIGPLVVKLQELAVSEARAMVAVNDDIRSLRDKLMWMQAFLRDADPRRRVVPDEASRVWLHQTRDAAFDAEDAVDQYFLLVDLSRYPSWTRTIIRFIAGLTTQIRVRRNLSSKINAINSRLENIIKNKEAYKQIGDTNKTVVPWRASTAISVAPTKLDNLLQPPLVSREDKRGQLISALLDSTHQKVISVIGPSGVGKSTLVRDVYETLEIKNHFVEQALATFPPYSSASDILKLILRDLKEEDFTLSKMEVTKELLDKKLKGKQYLVVIDGEVSSTEWKNILGALPNVAGSKVVRMSKENLEDPPTNYEHVVISLNRFDKIATTELFQQRVCKKESNPEYNKDIEDGVRNKYQQDIFDTTQGLPLAIVLLSGLLRTKEFPVEWEAVFDHLKSKQSKRLDSILSLCFDDLPYDIKSCFLYFAALPTNMLIEAQDLVCMWMAEGFLRPKEGLTMEKVGYRYLKELIARHLINLEPMDENTPEEELVTIQSKVHAFLQIEAQEVNFVEIHNSDDIPPLSAARRLSLQNRMEKYAALANPMPKLRSILSNFEKEETSKEDEVADDEEEAKVQQSPTCLHCSPHGTTTKCKEGDAKSYLQQLLQTSKFLRVINLQGLEVGDKLPDEIGNVVHLQYLAVTSCSLKEVPPSVGRLSSLQTLDVRDTEVKELPVPFWEIRTLRHVFGHRLILPKRVGVLKNLQTLDTVKPDAKYGWDRNTLSKMIQLRSLFIWELSKGHEKGLVAALKKLKYLVTLTIHGDSIPSAVFTTKTSLQRLEVMELDGMLIDMPRVVDMDIKSCLPNLLLLSLENTMVSQDFINKLAELPFLASLTLDGGSYKDEQLVLSAGGFHSLRRLTVDLEELKKLEIHESALPKLADLDILIHSNDLKIVILGKNDVVEKLLHEDKILSKKIQRTTRSERTARRIGVE

Target ORF information:

RefSeq Version	XM_002448943.2
Organism	Sorghum bicolor(sorghum)
Definition	Sorghum bicolor putative disease resistance RPP13-like protein 3 (LOC8069315), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002448943.2

ORF Insert Sequence:

ATGCCGTCAG GCTGGCTTTT ACCTTTATAT GCCATGGCAG AATCTGTTCC TAACTTCCTA 
TACATCTCGC GGGGTAACCG ATCGATCACC GACCGCACGT ATTCTGAATT TCTGATTCCT 
CTTTTCCATC ACGTTATGTG CCATGCATGC TTAATTAGCT TCCAGATGCA AATTGCCAAC 
TCGATCAAAG GGGTCGTCTT TATTTATTTG GGTTGGCATC GATCATTGGC AATTTGCATC 
GTCGGCCGGT GGTGTGAGAC TGAGAAGCTG CTGCAGGCCG GTGGAAGCAT GGCGGAAGCG 
ATCATCGGTC CCCTGGTGGT GAAGCTGCAG GAGCTCGCCG TCAGCGAGGC GCGGGCGATG 
GTGGCGGTGA ACGACGACAT CCGGAGCCTC CGGGACAAGC TCATGTGGAT GCAGGCCTTC 
CTGCGTGACG CCGACCCGCG GCGCCGCGTC GTCCCCGACG AGGCCAGCCG GGTGTGGCTC 
CACCAGACGC GCGACGCCGC CTTCGACGCC GAGGACGCCG TCGACCAGTA TTTCCTCCTC 
GTCGACCTAT CCAGGTACCC AAGCTGGACA CGCACCATTA TAAGGTTCAT TGCCGGATTA 
ACTACACAAA TACGTGTGCG GCGCAATCTT TCTAGCAAGA TCAATGCTAT CAACTCAAGA 
CTTGAGAATA TAATTAAGAA CAAGGAAGCA TACAAGCAAA TCGGGGACAC CAACAAGACA 
GTTGTTCCAT GGAGGGCTTC CACGGCCATC TCTGTTGCTC CAACCAAATT GGACAATTTG 
CTGCAGCCTC CTCTTGTTAG TCGTGAAGAC AAGCGCGGAC AGCTCATTTC TGCTCTGCTT 
GATAGCACTC ATCAAAAGGT TATCTCTGTG ATAGGGCCAA GTGGAGTGGG CAAGTCAACT 
CTAGTGAGAG ATGTGTATGA AACGTTGGAA ATCAAGAACC ATTTTGTTGA GCAGGCTTTG 
GCGACTTTCC CACCTTATTC CAGTGCTTCC GATATCCTGA AATTAATCCT TAGGGATCTC 
AAAGAAGAAG ACTTCACATT ATCTAAGATG GAGGTCACCA AGGAGCTGCT TGATAAGAAA 
TTAAAAGGGA AGCAGTACTT GGTGGTGATA GATGGGGAAG TGAGCTCCAC TGAATGGAAA 
AACATCCTAG GTGCCCTCCC TAATGTAGCA GGAAGTAAAG TTGTGAGAAT GTCAAAAGAG 
AACCTAGAAG ATCCTCCAAC AAACTATGAA CATGTTGTTA TCTCCCTTAA CCGTTTTGAT 
AAGATTGCTA CAACAGAACT GTTCCAGCAG AGAGTGTGCA AGAAAGAGAG CAATCCTGAG 
TACAACAAAG ACATCGAAGA TGGTGTTCGA AACAAGTACC AGCAAGATAT CTTTGACACC 
ACACAGGGGC TACCACTTGC AATTGTTCTT CTTTCAGGCC TTCTACGAAC AAAGGAGTTT 
CCAGTCGAGT GGGAAGCTGT GTTTGATCAT CTTAAATCCA AGCAATCAAA GAGGCTTGAC 
AGCATACTTT CCCTGTGCTT TGATGATCTT CCATATGACA TAAAATCTTG CTTCCTCTAC 
TTTGCTGCAT TGCCAACAAA CATGCTGATT GAAGCACAAG ATTTGGTGTG CATGTGGATG 
GCAGAGGGCT TCCTGAGGCC AAAGGAAGGG TTGACAATGG AGAAGGTTGG TTACCGGTAC 
TTGAAGGAGT TGATTGCAAG GCATTTAATC AACCTAGAGC CTATGGATGA GAATACCCCC 
GAGGAGGAGC TTGTGACCAT CCAAAGCAAA GTCCACGCAT TTCTTCAGAT TGAGGCACAA 
GAGGTAAATT TTGTGGAGAT CCACAACAGT GATGACATCC CACCTCTGTC AGCTGCTCGC 
CGCCTCAGCT TGCAAAATCG CATGGAGAAG TATGCTGCAC TAGCCAACCC TATGCCAAAG 
CTTCGATCCA TCTTGTCAAA TTTTGAGAAG GAAGAAACAA GCAAGGAAGA CGAAGTCGCC 
GACGATGAAG AGGAAGCCAA AGTGCAACAA TCTCCAACCT GCCTACATTG CTCACCACAT 
GGAACCACAA CCAAGTGCAA GGAGGGCGAT GCCAAGTCTT ATCTGCAGCA ACTTCTGCAA 
ACATCCAAGT TCCTACGTGT GATTAACCTG CAAGGCCTTG AAGTTGGTGA CAAGTTGCCA 
GATGAAATTG GTAATGTTGT GCACTTGCAG TACCTTGCTG TGACATCCTG TTCTTTGAAA 
GAGGTACCGC CATCAGTTGG AAGGCTCTCT AGTCTTCAGA CACTTGATGT CCGAGACACA 
GAAGTTAAGG AGCTACCGGT GCCATTCTGG GAGATTAGAA CCCTAAGGCA TGTGTTTGGC 
CATCGCCTCA TCTTGCCAAA ACGGGTTGGC GTCTTGAAGA ACCTACAGAC CCTTGATACT 
GTGAAACCTG ATGCTAAGTA TGGTTGGGAT AGAAATACCC TTTCAAAAAT GATCCAGCTC 
CGTTCCTTGT TTATTTGGGA ACTCTCCAAA GGTCATGAGA AGGGGCTAGT TGCTGCTCTG 
AAAAAACTCA AGTACCTTGT CACACTGACC ATACACGGTG ACAGTATTCC ATCGGCTGTT 
TTCACTACTA AAACCTCGCT TCAACGACTT GAGGTGATGG AACTGGATGG GATGCTTATT 
GACATGCCAC GTGTAGTGGA CATGGACATC AAATCATGCT TGCCCAATCT ACTTCTTTTG 
TCATTAGAGA ACACAATGGT GTCACAGGAC TTCATCAACA AGTTGGCTGA GTTGCCCTTC 
CTTGCTAGTC TCACTTTGGA TGGTGGATCA TATAAGGATG AACAACTTGT ATTATCTGCT 
GGTGGATTTC ATAGTTTGAG GAGGTTGACA GTGGACTTAG AAGAATTGAA GAAATTGGAA 
ATACATGAAT CAGCACTGCC CAAGCTTGCA GATTTGGATA TTTTGATCCA CTCTAATGAT 
CTCAAAATTG TGATACTGGG TAAGAACGAT GTCGTTGAGA AGCTCCTTCA TGAGGATAAA 
ATACTCTCCA AGAAAATCCA ACGGACTACG AGGAGTGAAC GAACTGCCCG AAGGATCGGA 
GTTGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Sorghum bicolor genome and the diversification of grasses.
Nature457(7229)551-6(2009 Jan)
Paterson AH,Bowers JE,Bruggmann R,Dubchak I,Grimwood J,Gundlach H,Haberer G,Hellsten U,Mitros T,Poliakov A,Schmutz J,Spannagl M,Tang H,Wang X,Wicker T,Bharti AK,Chapman J,Feltus FA,Gowik U,Grigoriev IV,Lyons E,Maher CA,Martis M,Narechania A,Otillar RP,Penning BW,Salamov AA,Wang Y,Zhang L,Carpita NC,Freeling M,Gingle AR,Hash CT,Keller B,Klein P,Kresovich S,McCann MC,Ming R,Peterson DG,Mehboob-ur-Rahman,Ware D,Westhoff P,Mayer KF,Messing J,Rokhsar DS

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SORBIDRAFT_05G002950 cDNA ORF clone, Sorghum bicolor(Sorghum)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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