ZNF654 cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene
Clones
gRNAs

Summary

Gene Symbol	ZNF654
Entrez Gene ID	100929570
Full Name	zinc finger protein 654
Gene Type	protein-coding
Organism	Sarcophilus harrisii(Tasmanian devil)

mRNA and Protein(s)

mRNA	Protein	Name
XM_023500297.2	XP_023356065.2	zinc finger protein 654 isoform X1
XM_031958847.1	XP_031814707.1	zinc finger protein 654 isoform X2
XM_023500297.1	XP_023356065.1	zinc finger protein 654 isoform X2
XM_012546042.1	XP_012401496.1	zinc finger protein 654
XM_023500296.1	XP_023356064.1	zinc finger protein 654 isoform X1

Pathways from BioSystems
Gene Ontology

The following ZNF654 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ZNF654 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSa127449	XM_012546042.1 Latest version!	Sarcophilus harrisii zinc finger protein 654 (ZNF654), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OSa127449	XM_023500296.1 Latest version!	Sarcophilus harrisii zinc finger protein 654 (ZNF654), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OSa152660	XM_023500297.1 Latest version!	Sarcophilus harrisii zinc finger protein 654 (ZNF654), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OSa229056	XM_023500297.2 Latest version!	Sarcophilus harrisii zinc finger protein 654 (ZNF654), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OSa229057	XM_031958847.1 Latest version!	Sarcophilus harrisii zinc finger protein 654 (ZNF654), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ZNF654 ( XM_012546042.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_012401496.1 Sarcophilus harrisii zinc finger protein 654 (ZNF654), mRNA.

Price & Availability↑

CloneID	OSa127449
Clone ID Related Accession (Same CDS sequence)	XM_012546042.1 , XM_023500296.1
Accession Version	XM_012546042.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2757bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1431532800000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	zinc finger protein 654
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003831808.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sarcophilus harrisii Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTTA TCAAATGTTG CATAAATCAC CCAGATATCA GTAAAGACTT GTACTTCCAT 
CAAGCATTCT TCACATGTCT GTATATGTCA CCATTAGAAG ATCACCTATT CCAGGAGCAT 
GTATTGAGAA CTGATTGTAA GAATGGAATT GATATCATCT GTAACACCGA AAAAGAAGGC 
AAGACTACAT TAGCCTTGCA GCTCTGTGAA TCATTCCTAA TTCCACAGCT CCAGAATGGG 
GACATGTATT GTATTTGGGA CTTGATTTTC ATATGGAGTA AACTGCAACT TAAATCTAAT 
CCTTCAAAGC AAGTTTTTGT AGACCAATGC TACCAGCTTT TAAGAACAGC AACTAATGTG 
AGAGTCATTT TTCCTTTCAT GAAAATAATA AAAGATGAGG TTGAAGTAGA AGGCTTACAA 
ATTTGTGTTG AAATATGTGG ATGTGCTCTA CAACTGGACC TTCAGGATGA TCCAAACACT 
AAATGTCTAA TTTATAAAAC AATTGCTCAT TTTTTGCCAA ATGATTTGGA GATTCTCAGA 
ATTTGTGCAC TCTCGGTATT TTTTCTTGAG CGCTCCTTGG AATCCTATTA TACTGTTGAA 
CGACTCTATA AACATCCAGA TGAAGAGTAC AATGAATGCA CTAGTTCTGT TCAGAATCGT 
GTTCGTTTTG AATTGCTTCC AATTTTGAAA AAGGGGTTGT TTTTTGATCC TGAATTTTGG 
AATTTCATAA TGATAAAGCA AAATTGTGCA GCATTATTGA GAGATAAATT AGCAGTTGGC 
TTTTTAAATG AAAATACACT GGAAAATACT ACCGGTCATC CTGGAAAGAC GTTAGAAACA 
CAGGTTTTAG ATGAAGATCT AGTCTTTCTT ATGGACCTGA ACAATGGGGA ATATGATCCA 
GAAGATGTCT CTGGAACCCA ATTCAAAGGT CATAGTAATG ACAAGAAAAA TCTCACAACT 
TTTAATGCTT CTAAGGTAGA CCGTAATGTT CCAAGGCATA AATGTGTGTT GTGTAACAGA 
GAATTTCTAG GTGGCCACAT TGTACGCCAT GCACAGGCTC ATCAGAAAAA GGGCAGCTTT 
TCATGTGTAA TTTGTGGTAG AAAATTTAGA AATAGAGGAC TTATGCTGAA GCATTTAAAG 
AATCATGTTA AGAAAATACA AAGACAGCAT TTTGCTGTAA TTCAACAGGA AGATCAGGGA 
ATTTATACAA CAGAACAAAT AAATTGCTCT GATGCTTCCA TTTCATTTGA AAATGGAAAT 
TCAAATATTA AAGATAGTGA AATAGAAACT CCTGTTACAT ATAACAGTGA AAATAAGAAA 
GAATCTTTTG AGCAAGTAGC TAAAATGACT AAAAATTTTA TGTATGGACC TCATGATGTT 
ATTAAAAATG GCAGTCCTGA TAATTCTTTA AATAATGTCC CAGAGCCTCT CACCACTGAA 
AGTTTACCCA CATGTATTGA TGATGATGAT GATGATGACG ATGATGATGA TGATGATGAG 
TTAAATCGTG AGACTCCAGT ACTTCATAAA ATAAATGGAA CTGTTTGCCC TCAAAAAGAT 
CTGGATATTG TAGATCAACA AGAAAATTTC AAGTGCCCTG CTAATGGATG TGTCAGAATA 
TTTAAAAAAA TAAGATTTCT GAATAAACAT GCAAGGAAAG CTCATCCAAC TGATTTGAAA 
GTACAACAAC ATATAATGAA GTGGAACAAA GGAAAGTGCC GATTTTGTCA GCGGAAATTT 
GAGGATTCTC AGCATTTTAT AGATCATTTG AAGAGACATA TTTATCCAAA TGTGTACTTT 
TGTTTACATT TTACTTGTAA TCAGAGATTT AAACTGTCAA CCGAGCTTGC AGAGCATACA 
AAAAGTCACG GTGTTTTTCA GGCTCAGTGT AGTTTTCCAG AATGCCATGA GCTTTTTGAA 
GAGCTTCCTT TGCTGTATGA ACATGAAGCT CAGCATTACT TAAATCCAAC ACCAGAACCT 
TTTGCTGAAA CAAGTGGAAC TGTTTTGGAT ACTCTTACAA ATCTTCAGTG TGAGAATCAG 
GAAAAAGACC TCTCTGGTAG TGAAAAAGAA ACTGTTGATT TGCCAGTTCC CACTTGGAAG 
TCAAGGAAAG ATTCTACAGA ACCAAAGACA TATATACAAA GTGTTGAAAA GAAGACGAAT 
ATTGTAGTTC AGAATGGGAA TGACCATTCT TCTGATGTCA CTGCTTCAGA TATAAGCTTC 
ATAGACCAAA AGATGACTGT TGTAGAGCCA AATTCTGAAA AAAGTAGTGT TATTAATGAC 
CAACTGTTAG TCAATGGACA TCGTGAATCA GAGCAGACAT CTTTGGTTTC ATCAGATGCT 
GCCCTGAAAA GTGAGACAAA TGGAACAAGG ACAGAAAATG GTTCCATTTC ACAGAATGTT 
GAAGTAGTAT CACAAGAACA CAGTCCCCTA ATAGTAGTCT CTCAATCACC TTCCAAACCA 
AATCCATCAA CTGAAAATAC TTCATATGGT TTAATTTTAA CGAAGCCATA TGTGAGACCA 
TTGCCTCCCA GTTATCTTGA TGAACGGTAC ATCAGTATGC CAAAACGTAG AAAAACTCTG 
ACTGATAAAG TAGATGCCCA TTCTGATCAA GATAGTGTTT ATAGCACATC ATCAGAGAGA 
TTTAGATGTG GCAACTGCTT GACCATCTAC TGTAATTCAG AAGCACTTGA GGCTCATCTT 
GCACAAAAGA AATGTCAGAC GCTGTTTGGA TTCGACTCAG ATGATGAAAG TGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012401496.1
CDS	525..3281
Translation	MALIKCCINHPDISKDLYFHQAFFTCLYMSPLEDHLFQEHVLRTDCKNGIDIICNTEKEGKTTLALQLCESFLIPQLQNGDMYCIWDLIFIWSKLQLKSNPSKQVFVDQCYQLLRTATNVRVIFPFMKIIKDEVEVEGLQICVEICGCALQLDLQDDPNTKCLIYKTIAHFLPNDLEILRICALSVFFLERSLESYYTVERLYKHPDEEYNECTSSVQNRVRFELLPILKKGLFFDPEFWNFIMIKQNCAALLRDKLAVGFLNENTLENTTGHPGKTLETQVLDEDLVFLMDLNNGEYDPEDVSGTQFKGHSNDKKNLTTFNASKVDRNVPRHKCVLCNREFLGGHIVRHAQAHQKKGSFSCVICGRKFRNRGLMLKHLKNHVKKIQRQHFAVIQQEDQGIYTTEQINCSDASISFENGNSNIKDSEIETPVTYNSENKKESFEQVAKMTKNFMYGPHDVIKNGSPDNSLNNVPEPLTTESLPTCIDDDDDDDDDDDDDELNRETPVLHKINGTVCPQKDLDIVDQQENFKCPANGCVRIFKKIRFLNKHARKAHPTDLKVQQHIMKWNKGKCRFCQRKFEDSQHFIDHLKRHIYPNVYFCLHFTCNQRFKLSTELAEHTKSHGVFQAQCSFPECHELFEELPLLYEHEAQHYLNPTPEPFAETSGTVLDTLTNLQCENQEKDLSGSEKETVDLPVPTWKSRKDSTEPKTYIQSVEKKTNIVVQNGNDHSSDVTASDISFIDQKMTVVEPNSEKSSVINDQLLVNGHRESEQTSLVSSDAALKSETNGTRTENGSISQNVEVVSQEHSPLIVVSQSPSKPNPSTENTSYGLILTKPYVRPLPPSYLDERYISMPKRRKTLTDKVDAHSDQDSVYSTSSERFRCGNCLTIYCNSEALEAHLAQKKCQTLFGFDSDDESA

Target ORF information:

RefSeq Version	XM_012546042.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii zinc finger protein 654 (ZNF654), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012546042.1

ORF Insert Sequence:

ATGGCTCTTA TCAAATGTTG CATAAATCAC CCAGATATCA GTAAAGACTT GTACTTCCAT 
CAAGCATTCT TCACATGTCT GTATATGTCA CCATTAGAAG ATCACCTATT CCAGGAGCAT 
GTATTGAGAA CTGATTGTAA GAATGGAATT GATATCATCT GTAACACCGA AAAAGAAGGC 
AAGACTACAT TAGCCTTGCA GCTCTGTGAA TCATTCCTAA TTCCACAGCT CCAGAATGGG 
GACATGTATT GTATTTGGGA CTTGATTTTC ATATGGAGTA AACTGCAACT TAAATCTAAT 
CCTTCAAAGC AAGTTTTTGT AGACCAATGC TACCAGCTTT TAAGAACAGC AACTAATGTG 
AGAGTCATTT TTCCTTTCAT GAAAATAATA AAAGATGAGG TTGAAGTAGA AGGCTTACAA 
ATTTGTGTTG AAATATGTGG ATGTGCTCTA CAACTGGACC TTCAGGATGA TCCAAACACT 
AAATGTCTAA TTTATAAAAC AATTGCTCAT TTTTTGCCAA ATGATTTGGA GATTCTCAGA 
ATTTGTGCAC TCTCGGTATT TTTTCTTGAG CGCTCCTTGG AATCCTATTA TACTGTTGAA 
CGACTCTATA AACATCCAGA TGAAGAGTAC AATGAATGCA CTAGTTCTGT TCAGAATCGT 
GTTCGTTTTG AATTGCTTCC AATTTTGAAA AAGGGGTTGT TTTTTGATCC TGAATTTTGG 
AATTTCATAA TGATAAAGCA AAATTGTGCA GCATTATTGA GAGATAAATT AGCAGTTGGC 
TTTTTAAATG AAAATACACT GGAAAATACT ACCGGTCATC CTGGAAAGAC GTTAGAAACA 
CAGGTTTTAG ATGAAGATCT AGTCTTTCTT ATGGACCTGA ACAATGGGGA ATATGATCCA 
GAAGATGTCT CTGGAACCCA ATTCAAAGGT CATAGTAATG ACAAGAAAAA TCTCACAACT 
TTTAATGCTT CTAAGGTAGA CCGTAATGTT CCAAGGCATA AATGTGTGTT GTGTAACAGA 
GAATTTCTAG GTGGCCACAT TGTACGCCAT GCACAGGCTC ATCAGAAAAA GGGCAGCTTT 
TCATGTGTAA TTTGTGGTAG AAAATTTAGA AATAGAGGAC TTATGCTGAA GCATTTAAAG 
AATCATGTTA AGAAAATACA AAGACAGCAT TTTGCTGTAA TTCAACAGGA AGATCAGGGA 
ATTTATACAA CAGAACAAAT AAATTGCTCT GATGCTTCCA TTTCATTTGA AAATGGAAAT 
TCAAATATTA AAGATAGTGA AATAGAAACT CCTGTTACAT ATAACAGTGA AAATAAGAAA 
GAATCTTTTG AGCAAGTAGC TAAAATGACT AAAAATTTTA TGTATGGACC TCATGATGTT 
ATTAAAAATG GCAGTCCTGA TAATTCTTTA AATAATGTCC CAGAGCCTCT CACCACTGAA 
AGTTTACCCA CATGTATTGA TGATGATGAT GATGATGACG ATGATGATGA TGATGATGAG 
TTAAATCGTG AGACTCCAGT ACTTCATAAA ATAAATGGAA CTGTTTGCCC TCAAAAAGAT 
CTGGATATTG TAGATCAACA AGAAAATTTC AAGTGCCCTG CTAATGGATG TGTCAGAATA 
TTTAAAAAAA TAAGATTTCT GAATAAACAT GCAAGGAAAG CTCATCCAAC TGATTTGAAA 
GTACAACAAC ATATAATGAA GTGGAACAAA GGAAAGTGCC GATTTTGTCA GCGGAAATTT 
GAGGATTCTC AGCATTTTAT AGATCATTTG AAGAGACATA TTTATCCAAA TGTGTACTTT 
TGTTTACATT TTACTTGTAA TCAGAGATTT AAACTGTCAA CCGAGCTTGC AGAGCATACA 
AAAAGTCACG GTGTTTTTCA GGCTCAGTGT AGTTTTCCAG AATGCCATGA GCTTTTTGAA 
GAGCTTCCTT TGCTGTATGA ACATGAAGCT CAGCATTACT TAAATCCAAC ACCAGAACCT 
TTTGCTGAAA CAAGTGGAAC TGTTTTGGAT ACTCTTACAA ATCTTCAGTG TGAGAATCAG 
GAAAAAGACC TCTCTGGTAG TGAAAAAGAA ACTGTTGATT TGCCAGTTCC CACTTGGAAG 
TCAAGGAAAG ATTCTACAGA ACCAAAGACA TATATACAAA GTGTTGAAAA GAAGACGAAT 
ATTGTAGTTC AGAATGGGAA TGACCATTCT TCTGATGTCA CTGCTTCAGA TATAAGCTTC 
ATAGACCAAA AGATGACTGT TGTAGAGCCA AATTCTGAAA AAAGTAGTGT TATTAATGAC 
CAACTGTTAG TCAATGGACA TCGTGAATCA GAGCAGACAT CTTTGGTTTC ATCAGATGCT 
GCCCTGAAAA GTGAGACAAA TGGAACAAGG ACAGAAAATG GTTCCATTTC ACAGAATGTT 
GAAGTAGTAT CACAAGAACA CAGTCCCCTA ATAGTAGTCT CTCAATCACC TTCCAAACCA 
AATCCATCAA CTGAAAATAC TTCATATGGT TTAATTTTAA CGAAGCCATA TGTGAGACCA 
TTGCCTCCCA GTTATCTTGA TGAACGGTAC ATCAGTATGC CAAAACGTAG AAAAACTCTG 
ACTGATAAAG TAGATGCCCA TTCTGATCAA GATAGTGTTT ATAGCACATC ATCAGAGAGA 
TTTAGATGTG GCAACTGCTT GACCATCTAC TGTAATTCAG AAGCACTTGA GGCTCATCTT 
GCACAAAAGA AATGTCAGAC GCTGTTTGGA TTCGACTCAG ATGATGAAAG TGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZNF654 ( XM_023500296.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_023356064.1 Sarcophilus harrisii zinc finger protein 654 (ZNF654), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSa127449
Clone ID Related Accession (Same CDS sequence)	XM_012546042.1 , XM_023500296.1
Accession Version	XM_023500296.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2757bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515513600000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	zinc finger protein 654 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003831808.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTTA TCAAATGTTG CATAAATCAC CCAGATATCA GTAAAGACTT GTACTTCCAT 
CAAGCATTCT TCACATGTCT GTATATGTCA CCATTAGAAG ATCACCTATT CCAGGAGCAT 
GTATTGAGAA CTGATTGTAA GAATGGAATT GATATCATCT GTAACACCGA AAAAGAAGGC 
AAGACTACAT TAGCCTTGCA GCTCTGTGAA TCATTCCTAA TTCCACAGCT CCAGAATGGG 
GACATGTATT GTATTTGGGA CTTGATTTTC ATATGGAGTA AACTGCAACT TAAATCTAAT 
CCTTCAAAGC AAGTTTTTGT AGACCAATGC TACCAGCTTT TAAGAACAGC AACTAATGTG 
AGAGTCATTT TTCCTTTCAT GAAAATAATA AAAGATGAGG TTGAAGTAGA AGGCTTACAA 
ATTTGTGTTG AAATATGTGG ATGTGCTCTA CAACTGGACC TTCAGGATGA TCCAAACACT 
AAATGTCTAA TTTATAAAAC AATTGCTCAT TTTTTGCCAA ATGATTTGGA GATTCTCAGA 
ATTTGTGCAC TCTCGGTATT TTTTCTTGAG CGCTCCTTGG AATCCTATTA TACTGTTGAA 
CGACTCTATA AACATCCAGA TGAAGAGTAC AATGAATGCA CTAGTTCTGT TCAGAATCGT 
GTTCGTTTTG AATTGCTTCC AATTTTGAAA AAGGGGTTGT TTTTTGATCC TGAATTTTGG 
AATTTCATAA TGATAAAGCA AAATTGTGCA GCATTATTGA GAGATAAATT AGCAGTTGGC 
TTTTTAAATG AAAATACACT GGAAAATACT ACCGGTCATC CTGGAAAGAC GTTAGAAACA 
CAGGTTTTAG ATGAAGATCT AGTCTTTCTT ATGGACCTGA ACAATGGGGA ATATGATCCA 
GAAGATGTCT CTGGAACCCA ATTCAAAGGT CATAGTAATG ACAAGAAAAA TCTCACAACT 
TTTAATGCTT CTAAGGTAGA CCGTAATGTT CCAAGGCATA AATGTGTGTT GTGTAACAGA 
GAATTTCTAG GTGGCCACAT TGTACGCCAT GCACAGGCTC ATCAGAAAAA GGGCAGCTTT 
TCATGTGTAA TTTGTGGTAG AAAATTTAGA AATAGAGGAC TTATGCTGAA GCATTTAAAG 
AATCATGTTA AGAAAATACA AAGACAGCAT TTTGCTGTAA TTCAACAGGA AGATCAGGGA 
ATTTATACAA CAGAACAAAT AAATTGCTCT GATGCTTCCA TTTCATTTGA AAATGGAAAT 
TCAAATATTA AAGATAGTGA AATAGAAACT CCTGTTACAT ATAACAGTGA AAATAAGAAA 
GAATCTTTTG AGCAAGTAGC TAAAATGACT AAAAATTTTA TGTATGGACC TCATGATGTT 
ATTAAAAATG GCAGTCCTGA TAATTCTTTA AATAATGTCC CAGAGCCTCT CACCACTGAA 
AGTTTACCCA CATGTATTGA TGATGATGAT GATGATGACG ATGATGATGA TGATGATGAG 
TTAAATCGTG AGACTCCAGT ACTTCATAAA ATAAATGGAA CTGTTTGCCC TCAAAAAGAT 
CTGGATATTG TAGATCAACA AGAAAATTTC AAGTGCCCTG CTAATGGATG TGTCAGAATA 
TTTAAAAAAA TAAGATTTCT GAATAAACAT GCAAGGAAAG CTCATCCAAC TGATTTGAAA 
GTACAACAAC ATATAATGAA GTGGAACAAA GGAAAGTGCC GATTTTGTCA GCGGAAATTT 
GAGGATTCTC AGCATTTTAT AGATCATTTG AAGAGACATA TTTATCCAAA TGTGTACTTT 
TGTTTACATT TTACTTGTAA TCAGAGATTT AAACTGTCAA CCGAGCTTGC AGAGCATACA 
AAAAGTCACG GTGTTTTTCA GGCTCAGTGT AGTTTTCCAG AATGCCATGA GCTTTTTGAA 
GAGCTTCCTT TGCTGTATGA ACATGAAGCT CAGCATTACT TAAATCCAAC ACCAGAACCT 
TTTGCTGAAA CAAGTGGAAC TGTTTTGGAT ACTCTTACAA ATCTTCAGTG TGAGAATCAG 
GAAAAAGACC TCTCTGGTAG TGAAAAAGAA ACTGTTGATT TGCCAGTTCC CACTTGGAAG 
TCAAGGAAAG ATTCTACAGA ACCAAAGACA TATATACAAA GTGTTGAAAA GAAGACGAAT 
ATTGTAGTTC AGAATGGGAA TGACCATTCT TCTGATGTCA CTGCTTCAGA TATAAGCTTC 
ATAGACCAAA AGATGACTGT TGTAGAGCCA AATTCTGAAA AAAGTAGTGT TATTAATGAC 
CAACTGTTAG TCAATGGACA TCGTGAATCA GAGCAGACAT CTTTGGTTTC ATCAGATGCT 
GCCCTGAAAA GTGAGACAAA TGGAACAAGG ACAGAAAATG GTTCCATTTC ACAGAATGTT 
GAAGTAGTAT CACAAGAACA CAGTCCCCTA ATAGTAGTCT CTCAATCACC TTCCAAACCA 
AATCCATCAA CTGAAAATAC TTCATATGGT TTAATTTTAA CGAAGCCATA TGTGAGACCA 
TTGCCTCCCA GTTATCTTGA TGAACGGTAC ATCAGTATGC CAAAACGTAG AAAAACTCTG 
ACTGATAAAG TAGATGCCCA TTCTGATCAA GATAGTGTTT ATAGCACATC ATCAGAGAGA 
TTTAGATGTG GCAACTGCTT GACCATCTAC TGTAATTCAG AAGCACTTGA GGCTCATCTT 
GCACAAAAGA AATGTCAGAC GCTGTTTGGA TTCGACTCAG ATGATGAAAG TGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023356064.1
CDS	167..2923
Translation	MALIKCCINHPDISKDLYFHQAFFTCLYMSPLEDHLFQEHVLRTDCKNGIDIICNTEKEGKTTLALQLCESFLIPQLQNGDMYCIWDLIFIWSKLQLKSNPSKQVFVDQCYQLLRTATNVRVIFPFMKIIKDEVEVEGLQICVEICGCALQLDLQDDPNTKCLIYKTIAHFLPNDLEILRICALSVFFLERSLESYYTVERLYKHPDEEYNECTSSVQNRVRFELLPILKKGLFFDPEFWNFIMIKQNCAALLRDKLAVGFLNENTLENTTGHPGKTLETQVLDEDLVFLMDLNNGEYDPEDVSGTQFKGHSNDKKNLTTFNASKVDRNVPRHKCVLCNREFLGGHIVRHAQAHQKKGSFSCVICGRKFRNRGLMLKHLKNHVKKIQRQHFAVIQQEDQGIYTTEQINCSDASISFENGNSNIKDSEIETPVTYNSENKKESFEQVAKMTKNFMYGPHDVIKNGSPDNSLNNVPEPLTTESLPTCIDDDDDDDDDDDDDELNRETPVLHKINGTVCPQKDLDIVDQQENFKCPANGCVRIFKKIRFLNKHARKAHPTDLKVQQHIMKWNKGKCRFCQRKFEDSQHFIDHLKRHIYPNVYFCLHFTCNQRFKLSTELAEHTKSHGVFQAQCSFPECHELFEELPLLYEHEAQHYLNPTPEPFAETSGTVLDTLTNLQCENQEKDLSGSEKETVDLPVPTWKSRKDSTEPKTYIQSVEKKTNIVVQNGNDHSSDVTASDISFIDQKMTVVEPNSEKSSVINDQLLVNGHRESEQTSLVSSDAALKSETNGTRTENGSISQNVEVVSQEHSPLIVVSQSPSKPNPSTENTSYGLILTKPYVRPLPPSYLDERYISMPKRRKTLTDKVDAHSDQDSVYSTSSERFRCGNCLTIYCNSEALEAHLAQKKCQTLFGFDSDDESA

Target ORF information:

RefSeq Version	XM_023500296.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii zinc finger protein 654 (ZNF654), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023500296.1

ORF Insert Sequence:

ATGGCTCTTA TCAAATGTTG CATAAATCAC CCAGATATCA GTAAAGACTT GTACTTCCAT 
CAAGCATTCT TCACATGTCT GTATATGTCA CCATTAGAAG ATCACCTATT CCAGGAGCAT 
GTATTGAGAA CTGATTGTAA GAATGGAATT GATATCATCT GTAACACCGA AAAAGAAGGC 
AAGACTACAT TAGCCTTGCA GCTCTGTGAA TCATTCCTAA TTCCACAGCT CCAGAATGGG 
GACATGTATT GTATTTGGGA CTTGATTTTC ATATGGAGTA AACTGCAACT TAAATCTAAT 
CCTTCAAAGC AAGTTTTTGT AGACCAATGC TACCAGCTTT TAAGAACAGC AACTAATGTG 
AGAGTCATTT TTCCTTTCAT GAAAATAATA AAAGATGAGG TTGAAGTAGA AGGCTTACAA 
ATTTGTGTTG AAATATGTGG ATGTGCTCTA CAACTGGACC TTCAGGATGA TCCAAACACT 
AAATGTCTAA TTTATAAAAC AATTGCTCAT TTTTTGCCAA ATGATTTGGA GATTCTCAGA 
ATTTGTGCAC TCTCGGTATT TTTTCTTGAG CGCTCCTTGG AATCCTATTA TACTGTTGAA 
CGACTCTATA AACATCCAGA TGAAGAGTAC AATGAATGCA CTAGTTCTGT TCAGAATCGT 
GTTCGTTTTG AATTGCTTCC AATTTTGAAA AAGGGGTTGT TTTTTGATCC TGAATTTTGG 
AATTTCATAA TGATAAAGCA AAATTGTGCA GCATTATTGA GAGATAAATT AGCAGTTGGC 
TTTTTAAATG AAAATACACT GGAAAATACT ACCGGTCATC CTGGAAAGAC GTTAGAAACA 
CAGGTTTTAG ATGAAGATCT AGTCTTTCTT ATGGACCTGA ACAATGGGGA ATATGATCCA 
GAAGATGTCT CTGGAACCCA ATTCAAAGGT CATAGTAATG ACAAGAAAAA TCTCACAACT 
TTTAATGCTT CTAAGGTAGA CCGTAATGTT CCAAGGCATA AATGTGTGTT GTGTAACAGA 
GAATTTCTAG GTGGCCACAT TGTACGCCAT GCACAGGCTC ATCAGAAAAA GGGCAGCTTT 
TCATGTGTAA TTTGTGGTAG AAAATTTAGA AATAGAGGAC TTATGCTGAA GCATTTAAAG 
AATCATGTTA AGAAAATACA AAGACAGCAT TTTGCTGTAA TTCAACAGGA AGATCAGGGA 
ATTTATACAA CAGAACAAAT AAATTGCTCT GATGCTTCCA TTTCATTTGA AAATGGAAAT 
TCAAATATTA AAGATAGTGA AATAGAAACT CCTGTTACAT ATAACAGTGA AAATAAGAAA 
GAATCTTTTG AGCAAGTAGC TAAAATGACT AAAAATTTTA TGTATGGACC TCATGATGTT 
ATTAAAAATG GCAGTCCTGA TAATTCTTTA AATAATGTCC CAGAGCCTCT CACCACTGAA 
AGTTTACCCA CATGTATTGA TGATGATGAT GATGATGACG ATGATGATGA TGATGATGAG 
TTAAATCGTG AGACTCCAGT ACTTCATAAA ATAAATGGAA CTGTTTGCCC TCAAAAAGAT 
CTGGATATTG TAGATCAACA AGAAAATTTC AAGTGCCCTG CTAATGGATG TGTCAGAATA 
TTTAAAAAAA TAAGATTTCT GAATAAACAT GCAAGGAAAG CTCATCCAAC TGATTTGAAA 
GTACAACAAC ATATAATGAA GTGGAACAAA GGAAAGTGCC GATTTTGTCA GCGGAAATTT 
GAGGATTCTC AGCATTTTAT AGATCATTTG AAGAGACATA TTTATCCAAA TGTGTACTTT 
TGTTTACATT TTACTTGTAA TCAGAGATTT AAACTGTCAA CCGAGCTTGC AGAGCATACA 
AAAAGTCACG GTGTTTTTCA GGCTCAGTGT AGTTTTCCAG AATGCCATGA GCTTTTTGAA 
GAGCTTCCTT TGCTGTATGA ACATGAAGCT CAGCATTACT TAAATCCAAC ACCAGAACCT 
TTTGCTGAAA CAAGTGGAAC TGTTTTGGAT ACTCTTACAA ATCTTCAGTG TGAGAATCAG 
GAAAAAGACC TCTCTGGTAG TGAAAAAGAA ACTGTTGATT TGCCAGTTCC CACTTGGAAG 
TCAAGGAAAG ATTCTACAGA ACCAAAGACA TATATACAAA GTGTTGAAAA GAAGACGAAT 
ATTGTAGTTC AGAATGGGAA TGACCATTCT TCTGATGTCA CTGCTTCAGA TATAAGCTTC 
ATAGACCAAA AGATGACTGT TGTAGAGCCA AATTCTGAAA AAAGTAGTGT TATTAATGAC 
CAACTGTTAG TCAATGGACA TCGTGAATCA GAGCAGACAT CTTTGGTTTC ATCAGATGCT 
GCCCTGAAAA GTGAGACAAA TGGAACAAGG ACAGAAAATG GTTCCATTTC ACAGAATGTT 
GAAGTAGTAT CACAAGAACA CAGTCCCCTA ATAGTAGTCT CTCAATCACC TTCCAAACCA 
AATCCATCAA CTGAAAATAC TTCATATGGT TTAATTTTAA CGAAGCCATA TGTGAGACCA 
TTGCCTCCCA GTTATCTTGA TGAACGGTAC ATCAGTATGC CAAAACGTAG AAAAACTCTG 
ACTGATAAAG TAGATGCCCA TTCTGATCAA GATAGTGTTT ATAGCACATC ATCAGAGAGA 
TTTAGATGTG GCAACTGCTT GACCATCTAC TGTAATTCAG AAGCACTTGA GGCTCATCTT 
GCACAAAAGA AATGTCAGAC GCTGTTTGGA TTCGACTCAG ATGATGAAAG TGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZNF654 ( XM_023500297.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_023356065.1 Sarcophilus harrisii zinc finger protein 654 (ZNF654), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSa152660
Clone ID Related Accession (Same CDS sequence)	XM_023500297.1
Accession Version	XM_023500297.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2799bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515513600000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	zinc finger protein 654 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003831808.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTTA TCAAATGTTG CATAAATCAC CCAGATATCA GTAAAGACTT GTACTTCCAT 
CAAGCATTCT TCACATGTCT GTATATGTCA CCATTAGAAG ATCACCTATT CCAGGAGCAT 
GTATTGAGAA CTGATTGTAA GAATGGAATT GATATCATCT GTAACACCGA AAAAGAAGGC 
AAGACTACAT TAGCCTTGCA GCTCTGTGAA TCATTCCTAA TTCCACAGCT CCAGAATGGG 
GACATGTATT GTATTTGGGA CTTGATTTTC ATATGGAGTA AACTGCAACT TAAATCTAAT 
CCTTCAAAGC AAGTTTTTGT AGACCAATGC TACCAGCTTT TAAGAACAGC AACTAATGTG 
AGAGTCATTT TTCCTTTCAT GAAAATAATA AAAGATGAGG TTGAAGTAGA AGGCTTACAA 
ATTTGTGTTG AAATATGTGG ATGTGCTCTA CAACTGGACC TTCAGGATGA TCCAAACACT 
AAATGTCTAA TTTATAAAAC AATTGCTCAT TTTTTGCCAA ATGATTTGGA GATTCTCAGA 
ATTTGTGCAC TCTCGGTATT TTTTCTTGAG CGCTCCTTGG AATCCTATTA TACTGTTGAA 
CGACTCTATA AACATCCAGA TGAAGAGTAC AATGAATGCA CTAGTTCTGT TCAGAATCGT 
GTTCGTTTTG AATTGCTTCC AATTTTGAAA AAGGGGTTGT TTTTTGATCC TGAATTTTGG 
AATTTCATAA TGATAAAGCA AAATTGTGCA GCATTATTGA GAGATAAATT AGCAGTTGGC 
TTTTTAAATG AAAATACACT GGAAAATACT ACCGGTCATC CTGGAAAGAC GTTAGAAACA 
CAGGTTTTAG ATGAAGATCT AGTCTTTCTT ATGGACCTGA ACAATGGGGA ATATGATCCA 
GAAGATGTCT CTGGAACCCA ATTCAAAGGT CATAGTAATG ACAAGAAAAA TCTCACAACT 
TTTAATGCTT CTAAGGTAGA CCGTAATGTT CCAAGGCATA AATGTGTGTT GTGTAACAGA 
GAATTTCTAG GTGGCCACAT TGTACGCCAT GCACAGGCTC ATCAGAAAAA GGGCAGCTTT 
TCATGTGTAA TTTGTGGTAG AAAATTTAGA AATAGAGGAC TTATGCTGAA GCATTTAAAG 
AATCATGTTA AGAAAATACA AAGACAGCAT TTTGCTGTAA TTCAACAGGA AGATCAGGGA 
ATTTATACAA CAGAACAAAT AAATTGCTCT GATGCTTCCA TTTCATTTGA AAATGGAAAT 
TCAAATATTA AAGATAGTGA AATAGAAACT CCTGTTACAT ATAACAGTGA AAATAAGAAA 
GAATCTTTTG AGCAAGTAGC TAAAATGACT AAAAATTTTA TGTATGGACC TCATGATGTT 
ATTAAAAATG GCAGTCCTGA TAATTCTTTA AATAATGTCC CAGAGCCTCT CACCACTGAA 
AGTTTACCCA CATGTATTGA TGATGATGAT GATGATGACG ATGATGATGA TGATGATGAG 
TTAAATCGTG AGACTCCAGT ACTTCATAAA ATAAATGGAA CTGTTTGCCC TCAAAAAGAT 
CTGGATATTG TAGATCAACA AGAAAATTTC AAGTGCCCTG CTAATGGATG TGTCAGAATA 
TTTAAAAAAA TAAGATTTCT GAATAAACAT GCAAGGAAAG CTCATCCAAC TGATTTGAAA 
GTACAACAAC ATATAATGAA GTGGAACAAA GGAAAGTGCC GATTTTGTCA GCGGAAATTT 
GAGGATTCTC AGCATTTTAT AGATCATTTG AAGAGACATA TTTATCCAAA TGTGTACTTT 
TGTTTACATT TTACTTGTAA TCAGAGATTT AAACTGTCAA CCGAGCTTGC AGAGCATACA 
AAAAGTCACG GTGTTTTTCA GGCTCAGTGT AGTTTTCCAG AATGCCATGA GCTTTTTGAA 
GAGCTTCCTT TGCTGTATGA ACATGAAGCT CAGCATTACT TAAATCCAAC ACCAGAACCT 
TTTGCTGAAA CAAGTGGAAC TGTTTTGGAT ACTCTTACAA ATCTTCAGTG TGAGAATCAG 
GAAAAAGACC TCTCTGGTAG TGAAAAAGAA ACTGTTGATT TGCCAGTTCC CACTTGGAAG 
TCAAGGAAAG ATTCTACAGA ACCAAAGACA TATATACAAA GTGTTGAAAA GAAGACGAAT 
ATTGTAGTTC AGAATGGGAA TGACCATTCT TCTGATGTCA CTGCTTCAGA TATAAGCTTC 
ATAGACCAAA AGATGACTGT TGTAGAGCCA AATTCTGAAA AAAGTAGTGT TATTAATGAC 
CAACTGTTAG TCAATGGACA TCGTGAATCA GAGCAGACAT CTTTGGTTTC ATCAGATGCT 
GCCCTGAAAA GTGAGACAAA TGGAACAAGG ACAGAAAATG GTTCCATTTC ACAGAATGTT 
GAAGTAGTAT CACAAGAACA CAGTCCCCTA ATAGTAGTCT CTCAATCACC TTCCAAACCA 
AATCCATCAA CTGAAAATAC TTCATATGGT TTAATTTTAA CGAAGCCATA TGTGAGACCA 
TTGCCTCCCA GTTATCTTGA TGAACGGTAC ATCAGTATGC CAAAACGTAG AAAAACTCTG 
ACTGATAAAG TAGATGCCCA TTCTGATCAA GATAGTGTTT ATAGCACATC ATCAGAGAGA 
TTTAGATGTG GCAACTGCTT GACCATCTAC TGTAATTCAG AAGCACTTGA GGCTCATCTT 
GCACAAAAGA AATGTCAGAC GCTGTTTGGA TTCGACTCAG ATGATGAAAG TAAGTCTTAT 
AAAATATGGT GGGAGTCAAG GGATTACATA AGTAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023356065.1
CDS	525..3323
Translation	MALIKCCINHPDISKDLYFHQAFFTCLYMSPLEDHLFQEHVLRTDCKNGIDIICNTEKEGKTTLALQLCESFLIPQLQNGDMYCIWDLIFIWSKLQLKSNPSKQVFVDQCYQLLRTATNVRVIFPFMKIIKDEVEVEGLQICVEICGCALQLDLQDDPNTKCLIYKTIAHFLPNDLEILRICALSVFFLERSLESYYTVERLYKHPDEEYNECTSSVQNRVRFELLPILKKGLFFDPEFWNFIMIKQNCAALLRDKLAVGFLNENTLENTTGHPGKTLETQVLDEDLVFLMDLNNGEYDPEDVSGTQFKGHSNDKKNLTTFNASKVDRNVPRHKCVLCNREFLGGHIVRHAQAHQKKGSFSCVICGRKFRNRGLMLKHLKNHVKKIQRQHFAVIQQEDQGIYTTEQINCSDASISFENGNSNIKDSEIETPVTYNSENKKESFEQVAKMTKNFMYGPHDVIKNGSPDNSLNNVPEPLTTESLPTCIDDDDDDDDDDDDDELNRETPVLHKINGTVCPQKDLDIVDQQENFKCPANGCVRIFKKIRFLNKHARKAHPTDLKVQQHIMKWNKGKCRFCQRKFEDSQHFIDHLKRHIYPNVYFCLHFTCNQRFKLSTELAEHTKSHGVFQAQCSFPECHELFEELPLLYEHEAQHYLNPTPEPFAETSGTVLDTLTNLQCENQEKDLSGSEKETVDLPVPTWKSRKDSTEPKTYIQSVEKKTNIVVQNGNDHSSDVTASDISFIDQKMTVVEPNSEKSSVINDQLLVNGHRESEQTSLVSSDAALKSETNGTRTENGSISQNVEVVSQEHSPLIVVSQSPSKPNPSTENTSYGLILTKPYVRPLPPSYLDERYISMPKRRKTLTDKVDAHSDQDSVYSTSSERFRCGNCLTIYCNSEALEAHLAQKKCQTLFGFDSDDESKSYKIWWESRDYISK

Target ORF information:

RefSeq Version	XM_023500297.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii zinc finger protein 654 (ZNF654), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023500297.1

ORF Insert Sequence:

ATGGCTCTTA TCAAATGTTG CATAAATCAC CCAGATATCA GTAAAGACTT GTACTTCCAT 
CAAGCATTCT TCACATGTCT GTATATGTCA CCATTAGAAG ATCACCTATT CCAGGAGCAT 
GTATTGAGAA CTGATTGTAA GAATGGAATT GATATCATCT GTAACACCGA AAAAGAAGGC 
AAGACTACAT TAGCCTTGCA GCTCTGTGAA TCATTCCTAA TTCCACAGCT CCAGAATGGG 
GACATGTATT GTATTTGGGA CTTGATTTTC ATATGGAGTA AACTGCAACT TAAATCTAAT 
CCTTCAAAGC AAGTTTTTGT AGACCAATGC TACCAGCTTT TAAGAACAGC AACTAATGTG 
AGAGTCATTT TTCCTTTCAT GAAAATAATA AAAGATGAGG TTGAAGTAGA AGGCTTACAA 
ATTTGTGTTG AAATATGTGG ATGTGCTCTA CAACTGGACC TTCAGGATGA TCCAAACACT 
AAATGTCTAA TTTATAAAAC AATTGCTCAT TTTTTGCCAA ATGATTTGGA GATTCTCAGA 
ATTTGTGCAC TCTCGGTATT TTTTCTTGAG CGCTCCTTGG AATCCTATTA TACTGTTGAA 
CGACTCTATA AACATCCAGA TGAAGAGTAC AATGAATGCA CTAGTTCTGT TCAGAATCGT 
GTTCGTTTTG AATTGCTTCC AATTTTGAAA AAGGGGTTGT TTTTTGATCC TGAATTTTGG 
AATTTCATAA TGATAAAGCA AAATTGTGCA GCATTATTGA GAGATAAATT AGCAGTTGGC 
TTTTTAAATG AAAATACACT GGAAAATACT ACCGGTCATC CTGGAAAGAC GTTAGAAACA 
CAGGTTTTAG ATGAAGATCT AGTCTTTCTT ATGGACCTGA ACAATGGGGA ATATGATCCA 
GAAGATGTCT CTGGAACCCA ATTCAAAGGT CATAGTAATG ACAAGAAAAA TCTCACAACT 
TTTAATGCTT CTAAGGTAGA CCGTAATGTT CCAAGGCATA AATGTGTGTT GTGTAACAGA 
GAATTTCTAG GTGGCCACAT TGTACGCCAT GCACAGGCTC ATCAGAAAAA GGGCAGCTTT 
TCATGTGTAA TTTGTGGTAG AAAATTTAGA AATAGAGGAC TTATGCTGAA GCATTTAAAG 
AATCATGTTA AGAAAATACA AAGACAGCAT TTTGCTGTAA TTCAACAGGA AGATCAGGGA 
ATTTATACAA CAGAACAAAT AAATTGCTCT GATGCTTCCA TTTCATTTGA AAATGGAAAT 
TCAAATATTA AAGATAGTGA AATAGAAACT CCTGTTACAT ATAACAGTGA AAATAAGAAA 
GAATCTTTTG AGCAAGTAGC TAAAATGACT AAAAATTTTA TGTATGGACC TCATGATGTT 
ATTAAAAATG GCAGTCCTGA TAATTCTTTA AATAATGTCC CAGAGCCTCT CACCACTGAA 
AGTTTACCCA CATGTATTGA TGATGATGAT GATGATGACG ATGATGATGA TGATGATGAG 
TTAAATCGTG AGACTCCAGT ACTTCATAAA ATAAATGGAA CTGTTTGCCC TCAAAAAGAT 
CTGGATATTG TAGATCAACA AGAAAATTTC AAGTGCCCTG CTAATGGATG TGTCAGAATA 
TTTAAAAAAA TAAGATTTCT GAATAAACAT GCAAGGAAAG CTCATCCAAC TGATTTGAAA 
GTACAACAAC ATATAATGAA GTGGAACAAA GGAAAGTGCC GATTTTGTCA GCGGAAATTT 
GAGGATTCTC AGCATTTTAT AGATCATTTG AAGAGACATA TTTATCCAAA TGTGTACTTT 
TGTTTACATT TTACTTGTAA TCAGAGATTT AAACTGTCAA CCGAGCTTGC AGAGCATACA 
AAAAGTCACG GTGTTTTTCA GGCTCAGTGT AGTTTTCCAG AATGCCATGA GCTTTTTGAA 
GAGCTTCCTT TGCTGTATGA ACATGAAGCT CAGCATTACT TAAATCCAAC ACCAGAACCT 
TTTGCTGAAA CAAGTGGAAC TGTTTTGGAT ACTCTTACAA ATCTTCAGTG TGAGAATCAG 
GAAAAAGACC TCTCTGGTAG TGAAAAAGAA ACTGTTGATT TGCCAGTTCC CACTTGGAAG 
TCAAGGAAAG ATTCTACAGA ACCAAAGACA TATATACAAA GTGTTGAAAA GAAGACGAAT 
ATTGTAGTTC AGAATGGGAA TGACCATTCT TCTGATGTCA CTGCTTCAGA TATAAGCTTC 
ATAGACCAAA AGATGACTGT TGTAGAGCCA AATTCTGAAA AAAGTAGTGT TATTAATGAC 
CAACTGTTAG TCAATGGACA TCGTGAATCA GAGCAGACAT CTTTGGTTTC ATCAGATGCT 
GCCCTGAAAA GTGAGACAAA TGGAACAAGG ACAGAAAATG GTTCCATTTC ACAGAATGTT 
GAAGTAGTAT CACAAGAACA CAGTCCCCTA ATAGTAGTCT CTCAATCACC TTCCAAACCA 
AATCCATCAA CTGAAAATAC TTCATATGGT TTAATTTTAA CGAAGCCATA TGTGAGACCA 
TTGCCTCCCA GTTATCTTGA TGAACGGTAC ATCAGTATGC CAAAACGTAG AAAAACTCTG 
ACTGATAAAG TAGATGCCCA TTCTGATCAA GATAGTGTTT ATAGCACATC ATCAGAGAGA 
TTTAGATGTG GCAACTGCTT GACCATCTAC TGTAATTCAG AAGCACTTGA GGCTCATCTT 
GCACAAAAGA AATGTCAGAC GCTGTTTGGA TTCGACTCAG ATGATGAAAG TAAGTCTTAT 
AAAATATGGT GGGAGTCAAG GGATTACATA AGTAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZNF654 ( XM_023500297.2 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_023356065.2 Sarcophilus harrisii zinc finger protein 654 (ZNF654), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSa229056
Clone ID Related Accession (Same CDS sequence)	XM_023500297.2
Accession Version	XM_023500297.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3435bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	zinc finger protein 654 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045428.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 31, 2019 this sequence version replaced XM_023500297.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGG AAGAGAGCGA CCAGGAGGCC GAGCGCCTCG GGGAAGAGCT TGAAGCGATC 
GTGGAATCCC CTCCGGGCTC CGTCGCGTTC GGAGCCGGGG CCGGGGGCGA TGGAGGAGGC 
AGCTGCGGCA CTGGAAGCAG CAGCAGCAGG ATCAGCAGTC GGGATTATTG CCGTCGCTTC 
TGTCAGGTGG TTGAAGATTA TGCTGGAAGA TGGCAAGTTC CTTTGCCACA GCTTCAGGTT 
CTTCAGACAG CACTTTGTTG TTTCACAACA GCTAGTGTAT CGTTCCCAAC TGAATGTGAG 
CACGTACAAT ATGTTTTGAG TAGCCTAGCT TTGAGTTTCT TTGAGTTGCT GCTGTTCTTT 
GGAAAAGATG AATTTTATGA AGAGCCATTA AAGAATATTC TTGGTTCAAT CCAGGAATGC 
CAGAACCACC TCAGTAGATA TGGAAATGTG AATCTCCAAC TGGTAACTCG AATTATTAGA 
GATGGTGGAC CATGGGAAGA TCCAGTCTTG CAAGCAGTTC TTAAAGCCAA GCCAACATCT 
CAGGATATAG TTAACAAATA TTTGAGCTCT GAAAATCCAC TGTTCTTTGA ACTGCGTGCC 
AGATACCTTA TTGCTTGTGA ACGTATACCA GAGGCAATGG CTCTTATCAA ATGTTGCATA 
AATCACCCAG ATATCAGTAA AGACTTGTAC TTCCATCAAG CATTCTTCAC ATGTCTGTAT 
ATGTCACCAT TAGAAGATCA CCTATTCCAG GAGCATGTAT TGAGAACTGA TTGTAAGAAT 
GGAATTGATA TCATCTGTAA CACCGAAAAA GAAGGCAAGA CTACATTAGC CTTGCAGCTC 
TGTGAATCAT TCCTAATTCC ACAGCTCCAG AATGGGGACA TGTATTGTAT TTGGGACTTG 
ATTTTCATAT GGAGTAAACT GCAACTTAAA TCTAATCCTT CAAAGCAAGT TTTTGTAGAC 
CAATGCTACC AGCTTTTAAG AACAGCAACT AATGTGAGAG TCATTTTTCC TTTCATGAAA 
ATAATAAAAG ATGAGGTTGA AGTAGAAGGC TTACAAATTT GTGTTGAAAT ATGTGGATGT 
GCTCTACAAC TGGACCTTCA GGATGATCCA AACACTAAAT GTCTAATTTA TAAAACAATT 
GCTCATTTTT TGCCAAATGA TTTGGAGATT CTCAGAATTT GTGCACTCTC TGTATTTTTT 
CTTGAGCGCT CCTTGGAATC CTATTATACT GTTGAACGAC TCTATAAACA TCCAGATGAA 
GAGTACAATG AATGCACTAG TTCTGTTCAG AATCGTGTTC GTTTTGAATT GCTTCCAATT 
TTGAAAAAGG GGTTGTTTTT TGATCCTGAA TTTTGGAATT TCATAATGAT AAAGCAAAAT 
TGTGCAGCAT TATTGAGAGA TAAATTAGCA GTTGGCTTTT TAAATGAAAA TACACTGGAA 
AATACTACCG GTCATCCTGG AAAGACGTTA GAAACACAGG TTTTAGATGA AGATCTAGTC 
TTTCTTATGG ACCTGAACAA TGGGGAATAT GATCCAGAAG ATGTCTCTGG AACCCAATTC 
AAAGGTCATA GTAATGACAA GAAAAATCTC ACAACTTTTA ATGCTTCTAA GGTAGACCGT 
AATGTTCCAA GGCATAAATG TGTGTTGTGT AACAGAGAAT TTCTAGGTGG CCACATTGTA 
CGCCATGCAC AGGCTCATCA GAAAAAGGGC AGCTTTTCAT GTGTAATTTG TGGTAGAAAA 
TTTAGAAATA GAGGACTTAT GCTGAAGCAT TTAAAGAATC ATGTTAAGAA AATACAAAGA 
CAGCATTTTG CTGTAATTCA ACAGGAAGAT CAGGGAATTT ATACAACAGA ACAAATAAAT 
TGCTCTGATG CTTCCATTTC ATTTGAAAAT GGAAATTCAA ATATTAAAGA TAGTGAAATA 
GAAACTCCTG TTACATATAA CAGTGAAAAT AAGAAAGAAT CTTTTGAGCA AGTAGCTAAA 
ATGACTAAAA ATTTTATGTA TGGACCTCAT GATGTTATTA AAAATGGCAG TCCTGATAAT 
TCTTTAAATA ATGTCCCAGA GCCTCTCACC ACTGAAAGTT TACCCACATG TATTGATGAT 
GATGATGATG ATGACGATGA TGATGATGAT GATGAGTTAA ATCGTGAGAC TCCAGTACTT 
CATAAAATAA ATGGAACTGT TTGCCCTCAA AAAGATCTGG ATATTGTAGA TCAACAAGAA 
AATTTCAAGT GCCCTGCTAA TGGATGTGTC AGAATATTTA AAAAAATAAG ATTTCTGAAT 
AAACATGCAA GGAAAGCTCA TCCAACTGAT TTGAAAGTAC AACAACATAT AATGAAGTGG 
AACAAAGGAA AGTGCCGATT TTGTCAGCGG AAATTTGAGG ATTCTCAGCA TTTTATAGAT 
CATTTGAAGA GACATATTTA TCCAAATGTG TACTTTTGTT TACATTTTAC TTGTAATCAG 
AGATTTAAAC TGTCAACCGA GCTTGCAGAG CATACAAAAA GTCACGGTGT TTTTCAGGCT 
CAGTGTAGTT TTCCAGAATG CCATGAGCTT TTTGAAGAGC TTCCTTTGCT GTATGAACAT 
GAAGCTCAGC ATTACTTAAA TCCAACACCA GAACCTTTTG CTGAAACAAG TGGAACTGTT 
TTGGATACTC TTACAAATCT TCAGTGTGAG AATCAGGAAA AAGACCTCTC TGGTAGTGAA 
AAAGAAACTG TTGATTTGCC AGTTCCCACT TGGAAGTCAA GGAAAGATTC TACAGAACCA 
AAGACATATA TACAAAGTGT TGAAAAGAAG ACGAATATTG TAGTTCAGAA TGGGAATGAC 
CATTCTTCTG ATGTCACTGC TTCAGATATA AGCTTCATAG ACCAAAAGAT GACTGTTGTA 
GAGCCAAATT CTGAAAAAAG TAGTGTTATT AATGACCAAC TGTTAGTCAA TGGACATCGT 
GAATCAGAGC AGACATCTTT GGTTTCATCA GATGCTGCCC TGAAAAGTGA GACAAATGGA 
ACAAGGACAG AAAATGGTTC CATTTCACAG AATGTTGAAG TAGTATCACA AGAACACAGT 
CCCCTAATAG TAGTCTCTCA ATCACCTTCC AAACCAAATC CATCAACTGA AAATACTTCA 
TATGGTTTAA TTTTAACGAA GCCATATGTG AGACCATTGC CTCCCAGTTA TCTTGATGAA 
CGGTACATCA GTATGCCAAA ACGTAGAAAA ACTCTGACTG ATAAAGTAGA TGCCCATTCT 
GATCAAGATA GTGTTTATAG CACATCATCA GAGAGATTTA GATGTGGCAA CTGCTTGACC 
ATCTACTGTA ATTCAGAAGC ACTTGAGGCT CATCTTGCAC AAAAGAAATG TCAGACGCTG 
TTTGGATTCG ACTCAGATGA TGAAAGTAAG TCTTATAAAA TATGGTGGGA GTCAAGGGAT 
TACATAAGTA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023356065.2
CDS	69..3503
Translation	MAEEESDQEAERLGEELEAIVESPPGSVAFGAGAGGDGGGSCGTGSSSSRISSRDYCRRFCQVVEDYAGRWQVPLPQLQVLQTALCCFTTASVSFPTECEHVQYVLSSLALSFFELLLFFGKDEFYEEPLKNILGSIQECQNHLSRYGNVNLQLVTRIIRDGGPWEDPVLQAVLKAKPTSQDIVNKYLSSENPLFFELRARYLIACERIPEAMALIKCCINHPDISKDLYFHQAFFTCLYMSPLEDHLFQEHVLRTDCKNGIDIICNTEKEGKTTLALQLCESFLIPQLQNGDMYCIWDLIFIWSKLQLKSNPSKQVFVDQCYQLLRTATNVRVIFPFMKIIKDEVEVEGLQICVEICGCALQLDLQDDPNTKCLIYKTIAHFLPNDLEILRICALSVFFLERSLESYYTVERLYKHPDEEYNECTSSVQNRVRFELLPILKKGLFFDPEFWNFIMIKQNCAALLRDKLAVGFLNENTLENTTGHPGKTLETQVLDEDLVFLMDLNNGEYDPEDVSGTQFKGHSNDKKNLTTFNASKVDRNVPRHKCVLCNREFLGGHIVRHAQAHQKKGSFSCVICGRKFRNRGLMLKHLKNHVKKIQRQHFAVIQQEDQGIYTTEQINCSDASISFENGNSNIKDSEIETPVTYNSENKKESFEQVAKMTKNFMYGPHDVIKNGSPDNSLNNVPEPLTTESLPTCIDDDDDDDDDDDDDELNRETPVLHKINGTVCPQKDLDIVDQQENFKCPANGCVRIFKKIRFLNKHARKAHPTDLKVQQHIMKWNKGKCRFCQRKFEDSQHFIDHLKRHIYPNVYFCLHFTCNQRFKLSTELAEHTKSHGVFQAQCSFPECHELFEELPLLYEHEAQHYLNPTPEPFAETSGTVLDTLTNLQCENQEKDLSGSEKETVDLPVPTWKSRKDSTEPKTYIQSVEKKTNIVVQNGNDHSSDVTASDISFIDQKMTVVEPNSEKSSVINDQLLVNGHRESEQTSLVSSDAALKSETNGTRTENGSISQNVEVVSQEHSPLIVVSQSPSKPNPSTENTSYGLILTKPYVRPLPPSYLDERYISMPKRRKTLTDKVDAHSDQDSVYSTSSERFRCGNCLTIYCNSEALEAHLAQKKCQTLFGFDSDDESKSYKIWWESRDYISK

Target ORF information:

RefSeq Version	XM_023500297.2
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii zinc finger protein 654 (ZNF654), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023500297.2

ORF Insert Sequence:

ATGGCGGAGG AAGAGAGCGA CCAGGAGGCC GAGCGCCTCG GGGAAGAGCT TGAAGCGATC 
GTGGAATCCC CTCCGGGCTC CGTCGCGTTC GGAGCCGGGG CCGGGGGCGA TGGAGGAGGC 
AGCTGCGGCA CTGGAAGCAG CAGCAGCAGG ATCAGCAGTC GGGATTATTG CCGTCGCTTC 
TGTCAGGTGG TTGAAGATTA TGCTGGAAGA TGGCAAGTTC CTTTGCCACA GCTTCAGGTT 
CTTCAGACAG CACTTTGTTG TTTCACAACA GCTAGTGTAT CGTTCCCAAC TGAATGTGAG 
CACGTACAAT ATGTTTTGAG TAGCCTAGCT TTGAGTTTCT TTGAGTTGCT GCTGTTCTTT 
GGAAAAGATG AATTTTATGA AGAGCCATTA AAGAATATTC TTGGTTCAAT CCAGGAATGC 
CAGAACCACC TCAGTAGATA TGGAAATGTG AATCTCCAAC TGGTAACTCG AATTATTAGA 
GATGGTGGAC CATGGGAAGA TCCAGTCTTG CAAGCAGTTC TTAAAGCCAA GCCAACATCT 
CAGGATATAG TTAACAAATA TTTGAGCTCT GAAAATCCAC TGTTCTTTGA ACTGCGTGCC 
AGATACCTTA TTGCTTGTGA ACGTATACCA GAGGCAATGG CTCTTATCAA ATGTTGCATA 
AATCACCCAG ATATCAGTAA AGACTTGTAC TTCCATCAAG CATTCTTCAC ATGTCTGTAT 
ATGTCACCAT TAGAAGATCA CCTATTCCAG GAGCATGTAT TGAGAACTGA TTGTAAGAAT 
GGAATTGATA TCATCTGTAA CACCGAAAAA GAAGGCAAGA CTACATTAGC CTTGCAGCTC 
TGTGAATCAT TCCTAATTCC ACAGCTCCAG AATGGGGACA TGTATTGTAT TTGGGACTTG 
ATTTTCATAT GGAGTAAACT GCAACTTAAA TCTAATCCTT CAAAGCAAGT TTTTGTAGAC 
CAATGCTACC AGCTTTTAAG AACAGCAACT AATGTGAGAG TCATTTTTCC TTTCATGAAA 
ATAATAAAAG ATGAGGTTGA AGTAGAAGGC TTACAAATTT GTGTTGAAAT ATGTGGATGT 
GCTCTACAAC TGGACCTTCA GGATGATCCA AACACTAAAT GTCTAATTTA TAAAACAATT 
GCTCATTTTT TGCCAAATGA TTTGGAGATT CTCAGAATTT GTGCACTCTC TGTATTTTTT 
CTTGAGCGCT CCTTGGAATC CTATTATACT GTTGAACGAC TCTATAAACA TCCAGATGAA 
GAGTACAATG AATGCACTAG TTCTGTTCAG AATCGTGTTC GTTTTGAATT GCTTCCAATT 
TTGAAAAAGG GGTTGTTTTT TGATCCTGAA TTTTGGAATT TCATAATGAT AAAGCAAAAT 
TGTGCAGCAT TATTGAGAGA TAAATTAGCA GTTGGCTTTT TAAATGAAAA TACACTGGAA 
AATACTACCG GTCATCCTGG AAAGACGTTA GAAACACAGG TTTTAGATGA AGATCTAGTC 
TTTCTTATGG ACCTGAACAA TGGGGAATAT GATCCAGAAG ATGTCTCTGG AACCCAATTC 
AAAGGTCATA GTAATGACAA GAAAAATCTC ACAACTTTTA ATGCTTCTAA GGTAGACCGT 
AATGTTCCAA GGCATAAATG TGTGTTGTGT AACAGAGAAT TTCTAGGTGG CCACATTGTA 
CGCCATGCAC AGGCTCATCA GAAAAAGGGC AGCTTTTCAT GTGTAATTTG TGGTAGAAAA 
TTTAGAAATA GAGGACTTAT GCTGAAGCAT TTAAAGAATC ATGTTAAGAA AATACAAAGA 
CAGCATTTTG CTGTAATTCA ACAGGAAGAT CAGGGAATTT ATACAACAGA ACAAATAAAT 
TGCTCTGATG CTTCCATTTC ATTTGAAAAT GGAAATTCAA ATATTAAAGA TAGTGAAATA 
GAAACTCCTG TTACATATAA CAGTGAAAAT AAGAAAGAAT CTTTTGAGCA AGTAGCTAAA 
ATGACTAAAA ATTTTATGTA TGGACCTCAT GATGTTATTA AAAATGGCAG TCCTGATAAT 
TCTTTAAATA ATGTCCCAGA GCCTCTCACC ACTGAAAGTT TACCCACATG TATTGATGAT 
GATGATGATG ATGACGATGA TGATGATGAT GATGAGTTAA ATCGTGAGAC TCCAGTACTT 
CATAAAATAA ATGGAACTGT TTGCCCTCAA AAAGATCTGG ATATTGTAGA TCAACAAGAA 
AATTTCAAGT GCCCTGCTAA TGGATGTGTC AGAATATTTA AAAAAATAAG ATTTCTGAAT 
AAACATGCAA GGAAAGCTCA TCCAACTGAT TTGAAAGTAC AACAACATAT AATGAAGTGG 
AACAAAGGAA AGTGCCGATT TTGTCAGCGG AAATTTGAGG ATTCTCAGCA TTTTATAGAT 
CATTTGAAGA GACATATTTA TCCAAATGTG TACTTTTGTT TACATTTTAC TTGTAATCAG 
AGATTTAAAC TGTCAACCGA GCTTGCAGAG CATACAAAAA GTCACGGTGT TTTTCAGGCT 
CAGTGTAGTT TTCCAGAATG CCATGAGCTT TTTGAAGAGC TTCCTTTGCT GTATGAACAT 
GAAGCTCAGC ATTACTTAAA TCCAACACCA GAACCTTTTG CTGAAACAAG TGGAACTGTT 
TTGGATACTC TTACAAATCT TCAGTGTGAG AATCAGGAAA AAGACCTCTC TGGTAGTGAA 
AAAGAAACTG TTGATTTGCC AGTTCCCACT TGGAAGTCAA GGAAAGATTC TACAGAACCA 
AAGACATATA TACAAAGTGT TGAAAAGAAG ACGAATATTG TAGTTCAGAA TGGGAATGAC 
CATTCTTCTG ATGTCACTGC TTCAGATATA AGCTTCATAG ACCAAAAGAT GACTGTTGTA 
GAGCCAAATT CTGAAAAAAG TAGTGTTATT AATGACCAAC TGTTAGTCAA TGGACATCGT 
GAATCAGAGC AGACATCTTT GGTTTCATCA GATGCTGCCC TGAAAAGTGA GACAAATGGA 
ACAAGGACAG AAAATGGTTC CATTTCACAG AATGTTGAAG TAGTATCACA AGAACACAGT 
CCCCTAATAG TAGTCTCTCA ATCACCTTCC AAACCAAATC CATCAACTGA AAATACTTCA 
TATGGTTTAA TTTTAACGAA GCCATATGTG AGACCATTGC CTCCCAGTTA TCTTGATGAA 
CGGTACATCA GTATGCCAAA ACGTAGAAAA ACTCTGACTG ATAAAGTAGA TGCCCATTCT 
GATCAAGATA GTGTTTATAG CACATCATCA GAGAGATTTA GATGTGGCAA CTGCTTGACC 
ATCTACTGTA ATTCAGAAGC ACTTGAGGCT CATCTTGCAC AAAAGAAATG TCAGACGCTG 
TTTGGATTCG ACTCAGATGA TGAAAGTAAG TCTTATAAAA TATGGTGGGA GTCAAGGGAT 
TACATAAGTA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZNF654 ( XM_031958847.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031814707.1 Sarcophilus harrisii zinc finger protein 654 (ZNF654), transcript variant X2, mRNA.

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CloneID	OSa229057
Clone ID Related Accession (Same CDS sequence)	XM_031958847.1
Accession Version	XM_031958847.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3393bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	zinc finger protein 654 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045428.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGG AAGAGAGCGA CCAGGAGGCC GAGCGCCTCG GGGAAGAGCT TGAAGCGATC 
GTGGAATCCC CTCCGGGCTC CGTCGCGTTC GGAGCCGGGG CCGGGGGCGA TGGAGGAGGC 
AGCTGCGGCA CTGGAAGCAG CAGCAGCAGG ATCAGCAGTC GGGATTATTG CCGTCGCTTC 
TGTCAGGTGG TTGAAGATTA TGCTGGAAGA TGGCAAGTTC CTTTGCCACA GCTTCAGGTT 
CTTCAGACAG CACTTTGTTG TTTCACAACA GCTAGTGTAT CGTTCCCAAC TGAATGTGAG 
CACGTACAAT ATGTTTTGAG TAGCCTAGCT TTGAGTTTCT TTGAGTTGCT GCTGTTCTTT 
GGAAAAGATG AATTTTATGA AGAGCCATTA AAGAATATTC TTGGTTCAAT CCAGGAATGC 
CAGAACCACC TCAGTAGATA TGGAAATGTG AATCTCCAAC TGGTAACTCG AATTATTAGA 
GATGGTGGAC CATGGGAAGA TCCAGTCTTG CAAGCAGTTC TTAAAGCCAA GCCAACATCT 
CAGGATATAG TTAACAAATA TTTGAGCTCT GAAAATCCAC TGTTCTTTGA ACTGCGTGCC 
AGATACCTTA TTGCTTGTGA ACGTATACCA GAGGCAATGG CTCTTATCAA ATGTTGCATA 
AATCACCCAG ATATCAGTAA AGACTTGTAC TTCCATCAAG CATTCTTCAC ATGTCTGTAT 
ATGTCACCAT TAGAAGATCA CCTATTCCAG GAGCATGTAT TGAGAACTGA TTGTAAGAAT 
GGAATTGATA TCATCTGTAA CACCGAAAAA GAAGGCAAGA CTACATTAGC CTTGCAGCTC 
TGTGAATCAT TCCTAATTCC ACAGCTCCAG AATGGGGACA TGTATTGTAT TTGGGACTTG 
ATTTTCATAT GGAGTAAACT GCAACTTAAA TCTAATCCTT CAAAGCAAGT TTTTGTAGAC 
CAATGCTACC AGCTTTTAAG AACAGCAACT AATGTGAGAG TCATTTTTCC TTTCATGAAA 
ATAATAAAAG ATGAGGTTGA AGTAGAAGGC TTACAAATTT GTGTTGAAAT ATGTGGATGT 
GCTCTACAAC TGGACCTTCA GGATGATCCA AACACTAAAT GTCTAATTTA TAAAACAATT 
GCTCATTTTT TGCCAAATGA TTTGGAGATT CTCAGAATTT GTGCACTCTC TGTATTTTTT 
CTTGAGCGCT CCTTGGAATC CTATTATACT GTTGAACGAC TCTATAAACA TCCAGATGAA 
GAGTACAATG AATGCACTAG TTCTGTTCAG AATCGTGTTC GTTTTGAATT GCTTCCAATT 
TTGAAAAAGG GGTTGTTTTT TGATCCTGAA TTTTGGAATT TCATAATGAT AAAGCAAAAT 
TGTGCAGCAT TATTGAGAGA TAAATTAGCA GTTGGCTTTT TAAATGAAAA TACACTGGAA 
AATACTACCG GTCATCCTGG AAAGACGTTA GAAACACAGG TTTTAGATGA AGATCTAGTC 
TTTCTTATGG ACCTGAACAA TGGGGAATAT GATCCAGAAG ATGTCTCTGG AACCCAATTC 
AAAGGTCATA GTAATGACAA GAAAAATCTC ACAACTTTTA ATGCTTCTAA GGTAGACCGT 
AATGTTCCAA GGCATAAATG TGTGTTGTGT AACAGAGAAT TTCTAGGTGG CCACATTGTA 
CGCCATGCAC AGGCTCATCA GAAAAAGGGC AGCTTTTCAT GTGTAATTTG TGGTAGAAAA 
TTTAGAAATA GAGGACTTAT GCTGAAGCAT TTAAAGAATC ATGTTAAGAA AATACAAAGA 
CAGCATTTTG CTGTAATTCA ACAGGAAGAT CAGGGAATTT ATACAACAGA ACAAATAAAT 
TGCTCTGATG CTTCCATTTC ATTTGAAAAT GGAAATTCAA ATATTAAAGA TAGTGAAATA 
GAAACTCCTG TTACATATAA CAGTGAAAAT AAGAAAGAAT CTTTTGAGCA AGTAGCTAAA 
ATGACTAAAA ATTTTATGTA TGGACCTCAT GATGTTATTA AAAATGGCAG TCCTGATAAT 
TCTTTAAATA ATGTCCCAGA GCCTCTCACC ACTGAAAGTT TACCCACATG TATTGATGAT 
GATGATGATG ATGACGATGA TGATGATGAT GATGAGTTAA ATCGTGAGAC TCCAGTACTT 
CATAAAATAA ATGGAACTGT TTGCCCTCAA AAAGATCTGG ATATTGTAGA TCAACAAGAA 
AATTTCAAGT GCCCTGCTAA TGGATGTGTC AGAATATTTA AAAAAATAAG ATTTCTGAAT 
AAACATGCAA GGAAAGCTCA TCCAACTGAT TTGAAAGTAC AACAACATAT AATGAAGTGG 
AACAAAGGAA AGTGCCGATT TTGTCAGCGG AAATTTGAGG ATTCTCAGCA TTTTATAGAT 
CATTTGAAGA GACATATTTA TCCAAATGTG TACTTTTGTT TACATTTTAC TTGTAATCAG 
AGATTTAAAC TGTCAACCGA GCTTGCAGAG CATACAAAAA GTCACGGTGT TTTTCAGGCT 
CAGTGTAGTT TTCCAGAATG CCATGAGCTT TTTGAAGAGC TTCCTTTGCT GTATGAACAT 
GAAGCTCAGC ATTACTTAAA TCCAACACCA GAACCTTTTG CTGAAACAAG TGGAACTGTT 
TTGGATACTC TTACAAATCT TCAGTGTGAG AATCAGGAAA AAGACCTCTC TGGTAGTGAA 
AAAGAAACTG TTGATTTGCC AGTTCCCACT TGGAAGTCAA GGAAAGATTC TACAGAACCA 
AAGACATATA TACAAAGTGT TGAAAAGAAG ACGAATATTG TAGTTCAGAA TGGGAATGAC 
CATTCTTCTG ATGTCACTGC TTCAGATATA AGCTTCATAG ACCAAAAGAT GACTGTTGTA 
GAGCCAAATT CTGAAAAAAG TAGTGTTATT AATGACCAAC TGTTAGTCAA TGGACATCGT 
GAATCAGAGC AGACATCTTT GGTTTCATCA GATGCTGCCC TGAAAAGTGA GACAAATGGA 
ACAAGGACAG AAAATGGTTC CATTTCACAG AATGTTGAAG TAGTATCACA AGAACACAGT 
CCCCTAATAG TAGTCTCTCA ATCACCTTCC AAACCAAATC CATCAACTGA AAATACTTCA 
TATGGTTTAA TTTTAACGAA GCCATATGTG AGACCATTGC CTCCCAGTTA TCTTGATGAA 
CGGTACATCA GTATGCCAAA ACGTAGAAAA ACTCTGACTG ATAAAGTAGA TGCCCATTCT 
GATCAAGATA GTGTTTATAG CACATCATCA GAGAGATTTA GATGTGGCAA CTGCTTGACC 
ATCTACTGTA ATTCAGAAGC ACTTGAGGCT CATCTTGCAC AAAAGAAATG TCAGACGCTG 
TTTGGATTCG ACTCAGATGA TGAAAGTGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031814707.1
CDS	69..3461
Translation	MAEEESDQEAERLGEELEAIVESPPGSVAFGAGAGGDGGGSCGTGSSSSRISSRDYCRRFCQVVEDYAGRWQVPLPQLQVLQTALCCFTTASVSFPTECEHVQYVLSSLALSFFELLLFFGKDEFYEEPLKNILGSIQECQNHLSRYGNVNLQLVTRIIRDGGPWEDPVLQAVLKAKPTSQDIVNKYLSSENPLFFELRARYLIACERIPEAMALIKCCINHPDISKDLYFHQAFFTCLYMSPLEDHLFQEHVLRTDCKNGIDIICNTEKEGKTTLALQLCESFLIPQLQNGDMYCIWDLIFIWSKLQLKSNPSKQVFVDQCYQLLRTATNVRVIFPFMKIIKDEVEVEGLQICVEICGCALQLDLQDDPNTKCLIYKTIAHFLPNDLEILRICALSVFFLERSLESYYTVERLYKHPDEEYNECTSSVQNRVRFELLPILKKGLFFDPEFWNFIMIKQNCAALLRDKLAVGFLNENTLENTTGHPGKTLETQVLDEDLVFLMDLNNGEYDPEDVSGTQFKGHSNDKKNLTTFNASKVDRNVPRHKCVLCNREFLGGHIVRHAQAHQKKGSFSCVICGRKFRNRGLMLKHLKNHVKKIQRQHFAVIQQEDQGIYTTEQINCSDASISFENGNSNIKDSEIETPVTYNSENKKESFEQVAKMTKNFMYGPHDVIKNGSPDNSLNNVPEPLTTESLPTCIDDDDDDDDDDDDDELNRETPVLHKINGTVCPQKDLDIVDQQENFKCPANGCVRIFKKIRFLNKHARKAHPTDLKVQQHIMKWNKGKCRFCQRKFEDSQHFIDHLKRHIYPNVYFCLHFTCNQRFKLSTELAEHTKSHGVFQAQCSFPECHELFEELPLLYEHEAQHYLNPTPEPFAETSGTVLDTLTNLQCENQEKDLSGSEKETVDLPVPTWKSRKDSTEPKTYIQSVEKKTNIVVQNGNDHSSDVTASDISFIDQKMTVVEPNSEKSSVINDQLLVNGHRESEQTSLVSSDAALKSETNGTRTENGSISQNVEVVSQEHSPLIVVSQSPSKPNPSTENTSYGLILTKPYVRPLPPSYLDERYISMPKRRKTLTDKVDAHSDQDSVYSTSSERFRCGNCLTIYCNSEALEAHLAQKKCQTLFGFDSDDESA

Target ORF information:

RefSeq Version	XM_031958847.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii zinc finger protein 654 (ZNF654), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031958847.1

ORF Insert Sequence:

ATGGCGGAGG AAGAGAGCGA CCAGGAGGCC GAGCGCCTCG GGGAAGAGCT TGAAGCGATC 
GTGGAATCCC CTCCGGGCTC CGTCGCGTTC GGAGCCGGGG CCGGGGGCGA TGGAGGAGGC 
AGCTGCGGCA CTGGAAGCAG CAGCAGCAGG ATCAGCAGTC GGGATTATTG CCGTCGCTTC 
TGTCAGGTGG TTGAAGATTA TGCTGGAAGA TGGCAAGTTC CTTTGCCACA GCTTCAGGTT 
CTTCAGACAG CACTTTGTTG TTTCACAACA GCTAGTGTAT CGTTCCCAAC TGAATGTGAG 
CACGTACAAT ATGTTTTGAG TAGCCTAGCT TTGAGTTTCT TTGAGTTGCT GCTGTTCTTT 
GGAAAAGATG AATTTTATGA AGAGCCATTA AAGAATATTC TTGGTTCAAT CCAGGAATGC 
CAGAACCACC TCAGTAGATA TGGAAATGTG AATCTCCAAC TGGTAACTCG AATTATTAGA 
GATGGTGGAC CATGGGAAGA TCCAGTCTTG CAAGCAGTTC TTAAAGCCAA GCCAACATCT 
CAGGATATAG TTAACAAATA TTTGAGCTCT GAAAATCCAC TGTTCTTTGA ACTGCGTGCC 
AGATACCTTA TTGCTTGTGA ACGTATACCA GAGGCAATGG CTCTTATCAA ATGTTGCATA 
AATCACCCAG ATATCAGTAA AGACTTGTAC TTCCATCAAG CATTCTTCAC ATGTCTGTAT 
ATGTCACCAT TAGAAGATCA CCTATTCCAG GAGCATGTAT TGAGAACTGA TTGTAAGAAT 
GGAATTGATA TCATCTGTAA CACCGAAAAA GAAGGCAAGA CTACATTAGC CTTGCAGCTC 
TGTGAATCAT TCCTAATTCC ACAGCTCCAG AATGGGGACA TGTATTGTAT TTGGGACTTG 
ATTTTCATAT GGAGTAAACT GCAACTTAAA TCTAATCCTT CAAAGCAAGT TTTTGTAGAC 
CAATGCTACC AGCTTTTAAG AACAGCAACT AATGTGAGAG TCATTTTTCC TTTCATGAAA 
ATAATAAAAG ATGAGGTTGA AGTAGAAGGC TTACAAATTT GTGTTGAAAT ATGTGGATGT 
GCTCTACAAC TGGACCTTCA GGATGATCCA AACACTAAAT GTCTAATTTA TAAAACAATT 
GCTCATTTTT TGCCAAATGA TTTGGAGATT CTCAGAATTT GTGCACTCTC TGTATTTTTT 
CTTGAGCGCT CCTTGGAATC CTATTATACT GTTGAACGAC TCTATAAACA TCCAGATGAA 
GAGTACAATG AATGCACTAG TTCTGTTCAG AATCGTGTTC GTTTTGAATT GCTTCCAATT 
TTGAAAAAGG GGTTGTTTTT TGATCCTGAA TTTTGGAATT TCATAATGAT AAAGCAAAAT 
TGTGCAGCAT TATTGAGAGA TAAATTAGCA GTTGGCTTTT TAAATGAAAA TACACTGGAA 
AATACTACCG GTCATCCTGG AAAGACGTTA GAAACACAGG TTTTAGATGA AGATCTAGTC 
TTTCTTATGG ACCTGAACAA TGGGGAATAT GATCCAGAAG ATGTCTCTGG AACCCAATTC 
AAAGGTCATA GTAATGACAA GAAAAATCTC ACAACTTTTA ATGCTTCTAA GGTAGACCGT 
AATGTTCCAA GGCATAAATG TGTGTTGTGT AACAGAGAAT TTCTAGGTGG CCACATTGTA 
CGCCATGCAC AGGCTCATCA GAAAAAGGGC AGCTTTTCAT GTGTAATTTG TGGTAGAAAA 
TTTAGAAATA GAGGACTTAT GCTGAAGCAT TTAAAGAATC ATGTTAAGAA AATACAAAGA 
CAGCATTTTG CTGTAATTCA ACAGGAAGAT CAGGGAATTT ATACAACAGA ACAAATAAAT 
TGCTCTGATG CTTCCATTTC ATTTGAAAAT GGAAATTCAA ATATTAAAGA TAGTGAAATA 
GAAACTCCTG TTACATATAA CAGTGAAAAT AAGAAAGAAT CTTTTGAGCA AGTAGCTAAA 
ATGACTAAAA ATTTTATGTA TGGACCTCAT GATGTTATTA AAAATGGCAG TCCTGATAAT 
TCTTTAAATA ATGTCCCAGA GCCTCTCACC ACTGAAAGTT TACCCACATG TATTGATGAT 
GATGATGATG ATGACGATGA TGATGATGAT GATGAGTTAA ATCGTGAGAC TCCAGTACTT 
CATAAAATAA ATGGAACTGT TTGCCCTCAA AAAGATCTGG ATATTGTAGA TCAACAAGAA 
AATTTCAAGT GCCCTGCTAA TGGATGTGTC AGAATATTTA AAAAAATAAG ATTTCTGAAT 
AAACATGCAA GGAAAGCTCA TCCAACTGAT TTGAAAGTAC AACAACATAT AATGAAGTGG 
AACAAAGGAA AGTGCCGATT TTGTCAGCGG AAATTTGAGG ATTCTCAGCA TTTTATAGAT 
CATTTGAAGA GACATATTTA TCCAAATGTG TACTTTTGTT TACATTTTAC TTGTAATCAG 
AGATTTAAAC TGTCAACCGA GCTTGCAGAG CATACAAAAA GTCACGGTGT TTTTCAGGCT 
CAGTGTAGTT TTCCAGAATG CCATGAGCTT TTTGAAGAGC TTCCTTTGCT GTATGAACAT 
GAAGCTCAGC ATTACTTAAA TCCAACACCA GAACCTTTTG CTGAAACAAG TGGAACTGTT 
TTGGATACTC TTACAAATCT TCAGTGTGAG AATCAGGAAA AAGACCTCTC TGGTAGTGAA 
AAAGAAACTG TTGATTTGCC AGTTCCCACT TGGAAGTCAA GGAAAGATTC TACAGAACCA 
AAGACATATA TACAAAGTGT TGAAAAGAAG ACGAATATTG TAGTTCAGAA TGGGAATGAC 
CATTCTTCTG ATGTCACTGC TTCAGATATA AGCTTCATAG ACCAAAAGAT GACTGTTGTA 
GAGCCAAATT CTGAAAAAAG TAGTGTTATT AATGACCAAC TGTTAGTCAA TGGACATCGT 
GAATCAGAGC AGACATCTTT GGTTTCATCA GATGCTGCCC TGAAAAGTGA GACAAATGGA 
ACAAGGACAG AAAATGGTTC CATTTCACAG AATGTTGAAG TAGTATCACA AGAACACAGT 
CCCCTAATAG TAGTCTCTCA ATCACCTTCC AAACCAAATC CATCAACTGA AAATACTTCA 
TATGGTTTAA TTTTAACGAA GCCATATGTG AGACCATTGC CTCCCAGTTA TCTTGATGAA 
CGGTACATCA GTATGCCAAA ACGTAGAAAA ACTCTGACTG ATAAAGTAGA TGCCCATTCT 
GATCAAGATA GTGTTTATAG CACATCATCA GAGAGATTTA GATGTGGCAA CTGCTTGACC 
ATCTACTGTA ATTCAGAAGC ACTTGAGGCT CATCTTGCAC AAAAGAAATG TCAGACGCTG 
TTTGGATTCG ACTCAGATGA TGAAAGTGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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ZNF654 cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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