CLSPN cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene
Clones
gRNAs

Summary

Gene Symbol	CLSPN
Entrez Gene ID	100925340
Full Name	claspin
Gene Type	protein-coding
Organism	Sarcophilus harrisii(Tasmanian devil)

mRNA and Protein(s)

mRNA	Protein	Name
XM_023500157.1	XP_023355925.1	claspin
XM_012545871.1	XP_012401325.1	claspin
XM_031962132.1	XP_031817992.1	claspin

Pathways from BioSystems
Gene Ontology

The following CLSPN gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CLSPN cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSa127248	XM_012545871.1 Latest version!	Sarcophilus harrisii claspin (CLSPN), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OSa152549	XM_023500157.1 Latest version!	Sarcophilus harrisii claspin (CLSPN), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OSa231203	XM_031962132.1 Latest version!	Sarcophilus harrisii claspin (CLSPN), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CLSPN ( XM_012545871.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_012401325.1 Sarcophilus harrisii claspin (CLSPN), mRNA.

Price & Availability↑

CloneID	OSa127248
Clone ID Related Accession (Same CDS sequence)	XM_012545871.1
Accession Version	XM_012545871.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3606bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1431532800000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	claspin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003831792.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sarcophilus harrisii Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 2% of CDS bases ##RefSeq-Attributes-END##

ATGGCAGGAG AGGTGGTGAC TGAGTTCCTC GCTGTCACGG AAAAAGTGTG GCTACATCAG 
AGATTCCCAA GGACTGAGAA ACTAAGCGAA GATGAAACCA AACAACCTTT CAATGATAGT 
GGCTGTCTTC TTGGAGATAG TGACCTTTTT GAGACTGGTT TGGAGGAAGA TCTCCCACCA 
GAAGATGAAG AATCATTAGA ATCCATACGA GCTTCTGTGA AAGGCAAAGT CAAAAAGCAC 
AAGAAAAAGG AGCTGTCTTT GGAAAGTGGG GGCTGTTCAT TTGAAGATGA AAAGGAGCTG 
TCAAAAGGAA TGATAAGAAA GGAGAGAAAG GCGGCCAAGC TAAGTAAGGA AGCTATGAAA 
AGGCTACATA GTGAGAGCCA ACGCCTTATT CGAGAGTCTG CCCTCTCTCT TCCCTATCAC 
ATGCCTGAAA ACAAAACCGT TCATGACTTC TTCAGAAGCA AGCCTCGACC TTCCGGCCAG 
GGAAACGCCA TGGCGCTGCT GAAGTCCTCT AAGTATCAGG CAGCCTTCAG TAAAGAAATC 
AGCACCACTG CTAGCTCAAG TGACATCAGC AGCACCAGCC ACTCCAGAGG CTCTAATCAA 
ATGGCGACGG GCCCTGACCT GGAAACTGAG AACAGCCCAC TGCCCTTAGC TCCCCAGGCC 
CCTGCCAACT CTGCTGACCA GCCTGCTGGT GAGGCTGGCA CAAGAGAGGA GTCAGGACGC 
TGTGGGGGTC GGGGGCAGAA GGAACTCGTG GAGGCCCCTA CTAGCCCTTC ACCCAAGGAC 
AGCTCAGAGT GTCAGGAGGA ATCTGGCGTC TTCAGGAGTA AGGAGCCAGC ACAGATGGAA 
TTGAGGGCCC CTGTGGCCCT TGAACTCAAT GTTCTAGAGG AAGAAGAAGA GCCTCAAGAT 
CCTAACAAAA CAAAGAGCAA GGTAGATGAC AGCAGGCAAC AAGCTGTGGG AAAGGCCACT 
ATTACATCAG AAAAAGGAAG GAAGGTCAGC TTCCTGGATA AACTCCAGCG ACTGGGAGTA 
GATCTCTCCA TTAAGCCAAA GCTAGGGGCA GATGAAGATT CTTTTGTGAC TCTTGACGAG 
CCTGAACCCA ATAAAGAACT GGAAGCTTTA AAGGAACGTT TCTGGAAGCA CGCATGTCCC 
ACAGCCAAGG CCCAAACTGT GTCTGAACGA AAAATGAACC TGAACATTAT AGTGAAGGAG 
ACTGGTGCGG ATGGCAAGGA AGAGCTGAAG GCTGATGTGG TCCCCCTGAC TTTGGCTACA 
GAAAAATTAG ATGGAATGAG CTATGCAAAA CCAGGTGAGA AGCTACAGGT GCTGAAAGCA 
AAACTCCAGG AAGCAATGAA ACTCCGAAGA CTTGAGGAGC GCCAGAAGCG GCAGGCGTTG 
TTCAAGCTGG ATAATGAGGA TGGGTTTGAG GAAGAGGAAG AGGAGGAGGA GGAGGAGGAG 
ATGACAGATG AATCAGATAA GGAAGAGGAG GAGAAGGAAG AAGAAGAGGA GGAGGAGGAG 
GAGGAGGAAG GCAATCAGGA GACAGGAGAA TATTTTCTTG ATGATGAAGA GGTAGAAGAC 
AAGGATATGA AAGAGGAAGA TAAAGAAATG GATAAGGAGA ACATTATTGA CAGCACAGAA 
ATTTTCAAGG CTGTTGATTG TAGTTCTATT CCTAAGCCTC CCACTTTGGA ATCTACCTTG 
CTCCTGTTCA AAGACAGCTC TTCCAAAATG GGATACTATC CTGCTGAAGA AAAATCTGAG 
ACTGATGAAA GTATAGGCAA AAGACCTAGT AAACTAGAAG AAGATGATTC ATGCTCATTG 
CTAACAAAGG AGAGCAGCCA CAACAGCAGC TTTGAGATGA TCGGCTCCAT GATTGCTCCC 
TATCAGCCTT GCAACAAGCA GTCAGGTCGT GGGACTAGTT TCCCCCCTGG GCTGGGGGGG 
TTCCGATCCC CCTCCCCTGG GTTATTTCGA GCAAGTTTGA TGAGCTCGAC TTCCAAGAGT 
TCAGGGAAGC TGTCTGAGCC TTCCCTTCCC ATAGAGGATT CCCAGGAGCT GTATAACGCC 
TCCCCGGAGC CGAAAACTCT CTTCCTGGGA GCAGGAGACT TCCAGTTCTG TTTGGAAGAT 
GACACTCAGA GCCAACTGTT AGATGCAGAT GGGTTCTTAA ATGTTGGAAA TCATCGAAAT 
AAATACCAGG CTTTGAAATC ACGGGTGCCT TTGGCCAGTA TGGATGAGAA TGCCATGGAT 
GCCAACATGG ATGAGCTGTT GGATTTGTGT ACAGGAAAGT TTAAGGATGA AAAACCTTTG 
CCTGAGAAGG ATGACAGAAA GGAAGACATG GAGGAACTTT TGGGTCTTTG TTCAGGGAAA 
TTCACTTCTC AGGGTTCTTC AACTCCTGCT CCTTCAGAGT CCTCCAAACA GGAGAAGGAC 
ATGAACACTG ATGACCCAAT GGCAGAAGCC CTTGCTCTTT GTTCAGGGTC CTTCCCAACA 
GATAAGGAAC TGGAAGGTGA AGAAGAAGAG GAGGAGTTTG GTGATTTTCA GCTTGTCACT 
GATAGCAATG TGTTTGATAG TGAAGAAGAG GAGCATTCTG AATCTGACAA CGAAACTTCT 
GAAGCCCAGG AAGATGATGA AGCTTCTGAA GCTCAGGAAG ATGATGAAGC TTCTGAAGCC 
CAGGAAGATG AGGAAGCTTC TGAACTGGAA GCCCATGAGG AAGATGATGA TGAGGAAGAA 
CTCTTGAAGC AATCTGAAAG ACAAAAAAGA CAAATGAGGC TGAAGAAGTA CCTGGAGGAT 
GAGGCTGAGG TGTCAGGGAG TGAAGTAGGA AGCGAGGATG AGTATGATGG GGAGGAGATC 
GATGAATACG AAGAAGATGT AATTGATGAA GAGCTTCCCT CAGATGAAGA ACTGCAGGAT 
CAGGTCAACA AAATACACAG GAAAGCTATG TTGGATGATG ACAAGCGACA ATTACGCTTA 
TACCAGGAGA GGTACCTTGC TGATGGAGAC CTGCACAGTG ATGGCCCTGG ACGAATGAGG 
AAATTCCGTT GGAAAAATAT CGATGATGCT TCCCAGATGG ACATGTTTCA CAGAGACTCT 
GATGAGGATC AGAATGAAGA ACAGTTTGAT GAAACAGAAG CCAAATGGAG GAAAGAGCGG 
ATTGAGCGAG AACAGTGGCT TAGAGACCAG GCTCAGCAGG GGAAAATTAC AGCTGAAGAG 
GATGATGAAA TTGGAGAAGA GAGTCAGTTT ATGATGCTGG CTAAGAAAGC CACTGCCAAA 
GCACTTCAGA AGAAAGCCAA CCATGTCACT GGTGCTCAGG AAGCAAAAAC CTTGCCCAGA 
AGTCCCTTTG AAGCCATCAG ACCTGGAAGT GCCCACCAGC TGAAGACAGG CTCACTGCTG 
AATCAACCCA AAACTCTGCT GCAGAAGTTA TCTGCCCTCT CTGACCTCAA CCCTAGTGCT 
CCTCGAAACT CTAGGAATTT TGTCTTCCAC ACACTCTCCC CAGTCAAGAA TGAGATGGCA 
AAGGAGCCTG CTAAGCCTCA GGTGAAGAGA AAGGGCATAC CTTTGGCCTC CCCATCTCCC 
AAGCACTTCA AAGCAAATGG CACTTTAGGA TCAAACCGGA GCATCTTTAA GTACTTGGAA 
AGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012401325.1
CDS	1..3606
Translation	MAGEVVTEFLAVTEKVWLHQRFPRTEKLSEDETKQPFNDSGCLLGDSDLFETGLEEDLPPEDEESLESIRASVKGKVKKHKKKELSLESGGCSFEDEKELSKGMIRKERKAAKLSKEAMKRLHSESQRLIRESALSLPYHMPENKTVHDFFRSKPRPSGQGNAMALLKSSKYQAAFSKEISTTASSSDISSTSHSRGSNQMATGPDLETENSPLPLAPQAPANSADQPAGEAGTREESGRCGGRGQKELVEAPTSPSPKDSSECQEESGVFRSKEPAQMELRAPVALELNVLEEEEEPQDPNKTKSKVDDSRQQAVGKATITSEKGRKVSFLDKLQRLGVDLSIKPKLGADEDSFVTLDEPEPNKELEALKERFWKHACPTAKAQTVSERKMNLNIIVKETGADGKEELKADVVPLTLATEKLDGMSYAKPGEKLQVLKAKLQEAMKLRRLEERQKRQALFKLDNEDGFEEEEEEEEEEEMTDESDKEEEEKEEEEEEEEEEEGNQETGEYFLDDEEVEDKDMKEEDKEMDKENIIDSTEIFKAVDCSSIPKPPTLESTLLLFKDSSSKMGYYPAEEKSETDESIGKRPSKLEEDDSCSLLTKESSHNSSFEMIGSMIAPYQPCNKQSGRGTSFPPGLGGFRSPSPGLFRASLMSSTSKSSGKLSEPSLPIEDSQELYNASPEPKTLFLGAGDFQFCLEDDTQSQLLDADGFLNVGNHRNKYQALKSRVPLASMDENAMDANMDELLDLCTGKFKDEKPLPEKDDRKEDMEELLGLCSGKFTSQGSSTPAPSESSKQEKDMNTDDPMAEALALCSGSFPTDKELEGEEEEEEFGDFQLVTDSNVFDSEEEEHSESDNETSEAQEDDEASEAQEDDEASEAQEDEEASELEAHEEDDDEEELLKQSERQKRQMRLKKYLEDEAEVSGSEVGSEDEYDGEEIDEYEEDVIDEELPSDEELQDQVNKIHRKAMLDDDKRQLRLYQERYLADGDLHSDGPGRMRKFRWKNIDDASQMDMFHRDSDEDQNEEQFDETEAKWRKERIEREQWLRDQAQQGKITAEEDDEIGEESQFMMLAKKATAKALQKKANHVTGAQEAKTLPRSPFEAIRPGSAHQLKTGSLLNQPKTLLQKLSALSDLNPSAPRNSRNFVFHTLSPVKNEMAKEPAKPQVKRKGIPLASPSPKHFKANGTLGSNRSIFKYLES

Target ORF information:

RefSeq Version	XM_012545871.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii claspin (CLSPN), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012545871.1

ORF Insert Sequence:

ATGGCAGGAG AGGTGGTGAC TGAGTTCCTC GCTGTCACGG AAAAAGTGTG GCTACATCAG 
AGATTCCCAA GGACTGAGAA ACTAAGCGAA GATGAAACCA AACAACCTTT CAATGATAGT 
GGCTGTCTTC TTGGAGATAG TGACCTTTTT GAGACTGGTT TGGAGGAAGA TCTCCCACCA 
GAAGATGAAG AATCATTAGA ATCCATACGA GCTTCTGTGA AAGGCAAAGT CAAAAAGCAC 
AAGAAAAAGG AGCTGTCTTT GGAAAGTGGG GGCTGTTCAT TTGAAGATGA AAAGGAGCTG 
TCAAAAGGAA TGATAAGAAA GGAGAGAAAG GCGGCCAAGC TAAGTAAGGA AGCTATGAAA 
AGGCTACATA GTGAGAGCCA ACGCCTTATT CGAGAGTCTG CCCTCTCTCT TCCCTATCAC 
ATGCCTGAAA ACAAAACCGT TCATGACTTC TTCAGAAGCA AGCCTCGACC TTCCGGCCAG 
GGAAACGCCA TGGCGCTGCT GAAGTCCTCT AAGTATCAGG CAGCCTTCAG TAAAGAAATC 
AGCACCACTG CTAGCTCAAG TGACATCAGC AGCACCAGCC ACTCCAGAGG CTCTAATCAA 
ATGGCGACGG GCCCTGACCT GGAAACTGAG AACAGCCCAC TGCCCTTAGC TCCCCAGGCC 
CCTGCCAACT CTGCTGACCA GCCTGCTGGT GAGGCTGGCA CAAGAGAGGA GTCAGGACGC 
TGTGGGGGTC GGGGGCAGAA GGAACTCGTG GAGGCCCCTA CTAGCCCTTC ACCCAAGGAC 
AGCTCAGAGT GTCAGGAGGA ATCTGGCGTC TTCAGGAGTA AGGAGCCAGC ACAGATGGAA 
TTGAGGGCCC CTGTGGCCCT TGAACTCAAT GTTCTAGAGG AAGAAGAAGA GCCTCAAGAT 
CCTAACAAAA CAAAGAGCAA GGTAGATGAC AGCAGGCAAC AAGCTGTGGG AAAGGCCACT 
ATTACATCAG AAAAAGGAAG GAAGGTCAGC TTCCTGGATA AACTCCAGCG ACTGGGAGTA 
GATCTCTCCA TTAAGCCAAA GCTAGGGGCA GATGAAGATT CTTTTGTGAC TCTTGACGAG 
CCTGAACCCA ATAAAGAACT GGAAGCTTTA AAGGAACGTT TCTGGAAGCA CGCATGTCCC 
ACAGCCAAGG CCCAAACTGT GTCTGAACGA AAAATGAACC TGAACATTAT AGTGAAGGAG 
ACTGGTGCGG ATGGCAAGGA AGAGCTGAAG GCTGATGTGG TCCCCCTGAC TTTGGCTACA 
GAAAAATTAG ATGGAATGAG CTATGCAAAA CCAGGTGAGA AGCTACAGGT GCTGAAAGCA 
AAACTCCAGG AAGCAATGAA ACTCCGAAGA CTTGAGGAGC GCCAGAAGCG GCAGGCGTTG 
TTCAAGCTGG ATAATGAGGA TGGGTTTGAG GAAGAGGAAG AGGAGGAGGA GGAGGAGGAG 
ATGACAGATG AATCAGATAA GGAAGAGGAG GAGAAGGAAG AAGAAGAGGA GGAGGAGGAG 
GAGGAGGAAG GCAATCAGGA GACAGGAGAA TATTTTCTTG ATGATGAAGA GGTAGAAGAC 
AAGGATATGA AAGAGGAAGA TAAAGAAATG GATAAGGAGA ACATTATTGA CAGCACAGAA 
ATTTTCAAGG CTGTTGATTG TAGTTCTATT CCTAAGCCTC CCACTTTGGA ATCTACCTTG 
CTCCTGTTCA AAGACAGCTC TTCCAAAATG GGATACTATC CTGCTGAAGA AAAATCTGAG 
ACTGATGAAA GTATAGGCAA AAGACCTAGT AAACTAGAAG AAGATGATTC ATGCTCATTG 
CTAACAAAGG AGAGCAGCCA CAACAGCAGC TTTGAGATGA TCGGCTCCAT GATTGCTCCC 
TATCAGCCTT GCAACAAGCA GTCAGGTCGT GGGACTAGTT TCCCCCCTGG GCTGGGGGGG 
TTCCGATCCC CCTCCCCTGG GTTATTTCGA GCAAGTTTGA TGAGCTCGAC TTCCAAGAGT 
TCAGGGAAGC TGTCTGAGCC TTCCCTTCCC ATAGAGGATT CCCAGGAGCT GTATAACGCC 
TCCCCGGAGC CGAAAACTCT CTTCCTGGGA GCAGGAGACT TCCAGTTCTG TTTGGAAGAT 
GACACTCAGA GCCAACTGTT AGATGCAGAT GGGTTCTTAA ATGTTGGAAA TCATCGAAAT 
AAATACCAGG CTTTGAAATC ACGGGTGCCT TTGGCCAGTA TGGATGAGAA TGCCATGGAT 
GCCAACATGG ATGAGCTGTT GGATTTGTGT ACAGGAAAGT TTAAGGATGA AAAACCTTTG 
CCTGAGAAGG ATGACAGAAA GGAAGACATG GAGGAACTTT TGGGTCTTTG TTCAGGGAAA 
TTCACTTCTC AGGGTTCTTC AACTCCTGCT CCTTCAGAGT CCTCCAAACA GGAGAAGGAC 
ATGAACACTG ATGACCCAAT GGCAGAAGCC CTTGCTCTTT GTTCAGGGTC CTTCCCAACA 
GATAAGGAAC TGGAAGGTGA AGAAGAAGAG GAGGAGTTTG GTGATTTTCA GCTTGTCACT 
GATAGCAATG TGTTTGATAG TGAAGAAGAG GAGCATTCTG AATCTGACAA CGAAACTTCT 
GAAGCCCAGG AAGATGATGA AGCTTCTGAA GCTCAGGAAG ATGATGAAGC TTCTGAAGCC 
CAGGAAGATG AGGAAGCTTC TGAACTGGAA GCCCATGAGG AAGATGATGA TGAGGAAGAA 
CTCTTGAAGC AATCTGAAAG ACAAAAAAGA CAAATGAGGC TGAAGAAGTA CCTGGAGGAT 
GAGGCTGAGG TGTCAGGGAG TGAAGTAGGA AGCGAGGATG AGTATGATGG GGAGGAGATC 
GATGAATACG AAGAAGATGT AATTGATGAA GAGCTTCCCT CAGATGAAGA ACTGCAGGAT 
CAGGTCAACA AAATACACAG GAAAGCTATG TTGGATGATG ACAAGCGACA ATTACGCTTA 
TACCAGGAGA GGTACCTTGC TGATGGAGAC CTGCACAGTG ATGGCCCTGG ACGAATGAGG 
AAATTCCGTT GGAAAAATAT CGATGATGCT TCCCAGATGG ACATGTTTCA CAGAGACTCT 
GATGAGGATC AGAATGAAGA ACAGTTTGAT GAAACAGAAG CCAAATGGAG GAAAGAGCGG 
ATTGAGCGAG AACAGTGGCT TAGAGACCAG GCTCAGCAGG GGAAAATTAC AGCTGAAGAG 
GATGATGAAA TTGGAGAAGA GAGTCAGTTT ATGATGCTGG CTAAGAAAGC CACTGCCAAA 
GCACTTCAGA AGAAAGCCAA CCATGTCACT GGTGCTCAGG AAGCAAAAAC CTTGCCCAGA 
AGTCCCTTTG AAGCCATCAG ACCTGGAAGT GCCCACCAGC TGAAGACAGG CTCACTGCTG 
AATCAACCCA AAACTCTGCT GCAGAAGTTA TCTGCCCTCT CTGACCTCAA CCCTAGTGCT 
CCTCGAAACT CTAGGAATTT TGTCTTCCAC ACACTCTCCC CAGTCAAGAA TGAGATGGCA 
AAGGAGCCTG CTAAGCCTCA GGTGAAGAGA AAGGGCATAC CTTTGGCCTC CCCATCTCCC 
AAGCACTTCA AAGCAAATGG CACTTTAGGA TCAAACCGGA GCATCTTTAA GTACTTGGAA 
AGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CLSPN ( XM_023500157.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_023355925.1 Sarcophilus harrisii claspin (CLSPN), mRNA.

Price & Availability↑

CloneID	OSa152549
Clone ID Related Accession (Same CDS sequence)	XM_023500157.1
Accession Version	XM_023500157.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515513600000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	claspin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003831792.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGGCAGGAG AGGTGGTGAC TGAGGAAGAT GAAACCAAAC AACCTTTCAA TGATAGTGGC 
TGTCTTCTTG GAGATAGTGA CCTTTTTGAG ACTGGTTTGG AGGAAGATCT CCCACCAGAA 
GATGAAGAAT CATTAGAATC CATACGAGCT TCTGTGAAAG GCAAAGTCAA AAAGCACAAG 
AAAAAGGAGC TGTCTTTGGA AAGTGGGGGC TGTTCATTTG AAGATGAAAA GGAGCTGTCA 
AAAGGAATGA TAAGAAAGGA GAGAAAGGCG GCCAAGCTAA GTAAGGAAGC TATGAAAAGG 
CTACATAGTG AGAGCCAACG CCTTATTCGA GAGTCTGCCC TCTCTCTTCC CTATCACATG 
CCTGAAAACA AAACCGTTCA TGACTTCTTC AGAAGCAAGC CTCGACCTTC CGGCCAGGGA 
AACGCCATGG CGCTGCTGAA GTCCTCTAAG TATCAGGCAG CCTTCAGTAA AGAAATCAGC 
ACCACTGCTA GCTCAAGTGA CATCAGCAGC ACCAGCCACT CCAGAGGCTC TAATCAAATG 
GCGACGGGCC CTGACCTGGA AACTGAGAAC AGCCCACTGC CCTTAGCTCC CCAGGCCCCT 
GCCAACTCTG CTGACCAGCC TGCTGGTGAG GCTGGCACAA GAGAGGAGTC AGGACGCTGT 
GGGGGTCGGG GGCAGAAGGA ACTCGTGGAG GCCCCTACTA GCCCTTCACC CAAGGACAGC 
TCAGAGTGTC AGGAGGAATC TGGCGTCTTC AGGAGTAAGG AGCCAGCACA GATGGAATTG 
AGGGCCCCTG TGGCCCTTGA ACTCAATGTT CTAGAGGAAG AAGAAGAGCC TCAAGATCCT 
AACAAAACAA AGAGCAAGGT AGATGACAGC AGGCAACAAG CTGTGGGAAA GGCCACTATT 
ACATCAGAAA AAGGAAGGAA GGTCAGCTTC CTGGATAAAC TCCAGCGACT GGGAGTAGAT 
CTCTCCATTA AGCCAAAGCT AGGGGCAGAT GAAGATTCTT TTGTGACTCT TGACGAGCCT 
GAACCCAATA AAGAACTGGA AGCTTTAAAG GAACGTTTCT GGAAGCACGC ATGTCCCACA 
GCCAAGGCCC AAACTGTGTC TGAACGAAAA ATGAACCTGA ACATTATAGT GAAGGAGACT 
GGTGCGGATG GCAAGGAAGA GCTGAAGGCT GATGTGGTCC CCCTGACTTT GGCTACAGAA 
AAATTAGATG GAATGAGCTA TGCAAAACCA GGTGAGAAGC TACAGGTGCT GAAAGCAAAA 
CTCCAGGAAG CAATGAAACT CCGAAGACTT GAGGAGCGCC AGAAGCGGCA GGCGTTGTTC 
AAGCTGGATA ATGAGGATGG GTTTGAGGAA GAGGAAGAGG AGGAGGAGGA GGAGGAGATG 
ACAGATGAAT CAGATAAGGA AGAGGAGGAG AAGGAAGAAG AAGAGGAGGA GGAGGAGGAG 
GAGGAAGGCA ATCAGGAGAC AGGAGAATAT TTTCTTGATG ATGAAGAGGT AGAAGACAAG 
GATATGAAAG AGGAAGATAA AGAAATGGAT AAGGAGAACA TTATTGACAG CACAGAAATT 
TTCAAGGCTG TTGATTGTAG TTCTATTCCT AAGCCTCCCA CTTTGGAATC TACCTTGCTC 
CTGTTCAAAG ACAGCTCTTC CAAAATGGGA TACTATCCTG CTGAAGAAAA ATCTGAGACT 
GATGAAAGTA TAGGCAAAAG ACCTAGTAAA CTAGAAGAAG ATGATTCATG CTCATTGCTA 
ACAAAGGAGA GCAGCCACAA CAGCAGCTTT GAGATGATCG GCTCCATGAT TGCTCCCTAT 
CAGCCTTGCA ACAAGCAGTC AGGTCGTGGG ACTAGTTTCC CCCCTGGGCT GGGGGGGTTC 
CGATCCCCCT CCCCTGGGTT ATTTCGAGCA AGTTTGATGA GCTCGACTTC CAAGAGTTCA 
GGGAAGCTGT CTGAGCCTTC CCTTCCCATA GAGGATTCCC AGGAGCTGTA TAACGCCTCC 
CCGGAGCCGA AAACTCTCTT CCTGGGAGCA GGAGACTTCC AGTTCTGTTT GGAAGATGAC 
ACTCAGAGCC AACTGTTAGA TGCAGATGGG TTCTTAAATG TTGGAAATCA TCGAAATAAA 
TACCAGGCTT TGAAATCACG GGTGCCTTTG GCCAGTATGG ATGAGAATGC CATGGATGCC 
AACATGGATG AGCTGTTGGA TTTGTGTACA GGAAAGTTTA AGGATGAAAA ACCTTTGCCT 
GAGAAGGATG ACAGAAAGGA AGACATGGAG GAACTTTTGG GTCTTTGTTC AGGGAAATTC 
ACTTCTCAGG GTTCTTCAAC TCCTGCTCCT TCAGAGTCCT CCAAACAGGA GAAGGACATG 
AACACTGATG ACCCAATGGC AGAAGCCCTT GCTCTTTGTT CAGGGTCCTT CCCAACAGAT 
AAGGAACTGG AAGGTGAAGA AGAAGAGGAG GAGTTTGGTG ATTTTCAGCT TGTCACTGAT 
AGCAATGTGT TTGATAGTGA AGAAGAGGAG CATTCTGAAT CTGACAACGA AACTTCTGAA 
GCCCAGGAAG ATGATGAAGC TTCTGAAGCT CAGGAAGATG ATGAAGCTTC TGAAGCCCAG 
GAAGATGAGG AAGCTTCTGA ACTGGAAGCC CATGAGGAAG ATGATGATGA GGAAGAACTC 
TTGAAGCAAT CTGAAAGACA AAAAAGACAA ATGAGGCTGA AGAAGTACCT GGAGGATGAG 
GCTGAGGTGT CAGGGAGTGA AGTAGGAAGC GAGGATGAGT ATGATGGGGA GGAGATCGAT 
GAATACGAAG AAGATGTAAT TGATGAAGAG CTTCCCTCAG ATGAAGAACT GCAGGATCAG 
GTCAACAAAA TACACAGGAA AGCTATGTTG GATGATGACA AGCGACAATT ACGCTTATAC 
CAGGAGAGGT ACCTTGCTGA TGGAGACCTG CACAGTGATG GCCCTGGACG AATGAGGAAA 
TTCCGTTGGA AAAATATCGA TGATGCTTCC CAGATGGACA TGTTTCACAG AGACTCTGAT 
GAGGATCAGA ATGAAGAACA GTTTGATGAA ACAGAAGCCA AATGGAGGAA AGAGCGGATT 
GAGCGAGAAC AGTGGCTTAG AGACCAGGCT CAGCAGGGGA AAATTACAGC TGAAGAGGAT 
GATGAAATTG GAGAAGAGAG TCAGTTTATG ATGCTGGCTA AGAAAGCCAC TGCCAAAGCA 
CTTCAGAAGA AAGCCAACCA TGTCACTGGT GCTCAGGAAG CAAAAACCTT GCCCAGAAGT 
CCCTTTGAAG CCATCAGACC TGGAAGTGCC CACCAGCTGA AGACAGGCTC ACTGCTGAAT 
CAACCCAAAA CTCTGCTGCA GAAGTTATCT GCCCTCTCTG ACCTCAACCC TAGTGCTCCT 
CGAAACTCTA GGAATTTTGT CTTCCACACA CTCTCCCCAG TCAAGAATGA GATGGCAAAG 
GAGCCTGCTA AGCCTCAGGT GAAGAGAAAG GGCATACCTT TGGCCTCCCC ATCTCCCAAG 
CACTTCAAAG CAAATGGCAC TTTAGGATCA AACCGGAGCA TCTTTAAGTA CTTGGAAAGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023355925.1
CDS	1..3543
Translation	MAGEVVTEEDETKQPFNDSGCLLGDSDLFETGLEEDLPPEDEESLESIRASVKGKVKKHKKKELSLESGGCSFEDEKELSKGMIRKERKAAKLSKEAMKRLHSESQRLIRESALSLPYHMPENKTVHDFFRSKPRPSGQGNAMALLKSSKYQAAFSKEISTTASSSDISSTSHSRGSNQMATGPDLETENSPLPLAPQAPANSADQPAGEAGTREESGRCGGRGQKELVEAPTSPSPKDSSECQEESGVFRSKEPAQMELRAPVALELNVLEEEEEPQDPNKTKSKVDDSRQQAVGKATITSEKGRKVSFLDKLQRLGVDLSIKPKLGADEDSFVTLDEPEPNKELEALKERFWKHACPTAKAQTVSERKMNLNIIVKETGADGKEELKADVVPLTLATEKLDGMSYAKPGEKLQVLKAKLQEAMKLRRLEERQKRQALFKLDNEDGFEEEEEEEEEEEMTDESDKEEEEKEEEEEEEEEEEGNQETGEYFLDDEEVEDKDMKEEDKEMDKENIIDSTEIFKAVDCSSIPKPPTLESTLLLFKDSSSKMGYYPAEEKSETDESIGKRPSKLEEDDSCSLLTKESSHNSSFEMIGSMIAPYQPCNKQSGRGTSFPPGLGGFRSPSPGLFRASLMSSTSKSSGKLSEPSLPIEDSQELYNASPEPKTLFLGAGDFQFCLEDDTQSQLLDADGFLNVGNHRNKYQALKSRVPLASMDENAMDANMDELLDLCTGKFKDEKPLPEKDDRKEDMEELLGLCSGKFTSQGSSTPAPSESSKQEKDMNTDDPMAEALALCSGSFPTDKELEGEEEEEEFGDFQLVTDSNVFDSEEEEHSESDNETSEAQEDDEASEAQEDDEASEAQEDEEASELEAHEEDDDEEELLKQSERQKRQMRLKKYLEDEAEVSGSEVGSEDEYDGEEIDEYEEDVIDEELPSDEELQDQVNKIHRKAMLDDDKRQLRLYQERYLADGDLHSDGPGRMRKFRWKNIDDASQMDMFHRDSDEDQNEEQFDETEAKWRKERIEREQWLRDQAQQGKITAEEDDEIGEESQFMMLAKKATAKALQKKANHVTGAQEAKTLPRSPFEAIRPGSAHQLKTGSLLNQPKTLLQKLSALSDLNPSAPRNSRNFVFHTLSPVKNEMAKEPAKPQVKRKGIPLASPSPKHFKANGTLGSNRSIFKYLES

Target ORF information:

RefSeq Version	XM_023500157.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii claspin (CLSPN), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023500157.1

ORF Insert Sequence:

ATGGCAGGAG AGGTGGTGAC TGAGGAAGAT GAAACCAAAC AACCTTTCAA TGATAGTGGC 
TGTCTTCTTG GAGATAGTGA CCTTTTTGAG ACTGGTTTGG AGGAAGATCT CCCACCAGAA 
GATGAAGAAT CATTAGAATC CATACGAGCT TCTGTGAAAG GCAAAGTCAA AAAGCACAAG 
AAAAAGGAGC TGTCTTTGGA AAGTGGGGGC TGTTCATTTG AAGATGAAAA GGAGCTGTCA 
AAAGGAATGA TAAGAAAGGA GAGAAAGGCG GCCAAGCTAA GTAAGGAAGC TATGAAAAGG 
CTACATAGTG AGAGCCAACG CCTTATTCGA GAGTCTGCCC TCTCTCTTCC CTATCACATG 
CCTGAAAACA AAACCGTTCA TGACTTCTTC AGAAGCAAGC CTCGACCTTC CGGCCAGGGA 
AACGCCATGG CGCTGCTGAA GTCCTCTAAG TATCAGGCAG CCTTCAGTAA AGAAATCAGC 
ACCACTGCTA GCTCAAGTGA CATCAGCAGC ACCAGCCACT CCAGAGGCTC TAATCAAATG 
GCGACGGGCC CTGACCTGGA AACTGAGAAC AGCCCACTGC CCTTAGCTCC CCAGGCCCCT 
GCCAACTCTG CTGACCAGCC TGCTGGTGAG GCTGGCACAA GAGAGGAGTC AGGACGCTGT 
GGGGGTCGGG GGCAGAAGGA ACTCGTGGAG GCCCCTACTA GCCCTTCACC CAAGGACAGC 
TCAGAGTGTC AGGAGGAATC TGGCGTCTTC AGGAGTAAGG AGCCAGCACA GATGGAATTG 
AGGGCCCCTG TGGCCCTTGA ACTCAATGTT CTAGAGGAAG AAGAAGAGCC TCAAGATCCT 
AACAAAACAA AGAGCAAGGT AGATGACAGC AGGCAACAAG CTGTGGGAAA GGCCACTATT 
ACATCAGAAA AAGGAAGGAA GGTCAGCTTC CTGGATAAAC TCCAGCGACT GGGAGTAGAT 
CTCTCCATTA AGCCAAAGCT AGGGGCAGAT GAAGATTCTT TTGTGACTCT TGACGAGCCT 
GAACCCAATA AAGAACTGGA AGCTTTAAAG GAACGTTTCT GGAAGCACGC ATGTCCCACA 
GCCAAGGCCC AAACTGTGTC TGAACGAAAA ATGAACCTGA ACATTATAGT GAAGGAGACT 
GGTGCGGATG GCAAGGAAGA GCTGAAGGCT GATGTGGTCC CCCTGACTTT GGCTACAGAA 
AAATTAGATG GAATGAGCTA TGCAAAACCA GGTGAGAAGC TACAGGTGCT GAAAGCAAAA 
CTCCAGGAAG CAATGAAACT CCGAAGACTT GAGGAGCGCC AGAAGCGGCA GGCGTTGTTC 
AAGCTGGATA ATGAGGATGG GTTTGAGGAA GAGGAAGAGG AGGAGGAGGA GGAGGAGATG 
ACAGATGAAT CAGATAAGGA AGAGGAGGAG AAGGAAGAAG AAGAGGAGGA GGAGGAGGAG 
GAGGAAGGCA ATCAGGAGAC AGGAGAATAT TTTCTTGATG ATGAAGAGGT AGAAGACAAG 
GATATGAAAG AGGAAGATAA AGAAATGGAT AAGGAGAACA TTATTGACAG CACAGAAATT 
TTCAAGGCTG TTGATTGTAG TTCTATTCCT AAGCCTCCCA CTTTGGAATC TACCTTGCTC 
CTGTTCAAAG ACAGCTCTTC CAAAATGGGA TACTATCCTG CTGAAGAAAA ATCTGAGACT 
GATGAAAGTA TAGGCAAAAG ACCTAGTAAA CTAGAAGAAG ATGATTCATG CTCATTGCTA 
ACAAAGGAGA GCAGCCACAA CAGCAGCTTT GAGATGATCG GCTCCATGAT TGCTCCCTAT 
CAGCCTTGCA ACAAGCAGTC AGGTCGTGGG ACTAGTTTCC CCCCTGGGCT GGGGGGGTTC 
CGATCCCCCT CCCCTGGGTT ATTTCGAGCA AGTTTGATGA GCTCGACTTC CAAGAGTTCA 
GGGAAGCTGT CTGAGCCTTC CCTTCCCATA GAGGATTCCC AGGAGCTGTA TAACGCCTCC 
CCGGAGCCGA AAACTCTCTT CCTGGGAGCA GGAGACTTCC AGTTCTGTTT GGAAGATGAC 
ACTCAGAGCC AACTGTTAGA TGCAGATGGG TTCTTAAATG TTGGAAATCA TCGAAATAAA 
TACCAGGCTT TGAAATCACG GGTGCCTTTG GCCAGTATGG ATGAGAATGC CATGGATGCC 
AACATGGATG AGCTGTTGGA TTTGTGTACA GGAAAGTTTA AGGATGAAAA ACCTTTGCCT 
GAGAAGGATG ACAGAAAGGA AGACATGGAG GAACTTTTGG GTCTTTGTTC AGGGAAATTC 
ACTTCTCAGG GTTCTTCAAC TCCTGCTCCT TCAGAGTCCT CCAAACAGGA GAAGGACATG 
AACACTGATG ACCCAATGGC AGAAGCCCTT GCTCTTTGTT CAGGGTCCTT CCCAACAGAT 
AAGGAACTGG AAGGTGAAGA AGAAGAGGAG GAGTTTGGTG ATTTTCAGCT TGTCACTGAT 
AGCAATGTGT TTGATAGTGA AGAAGAGGAG CATTCTGAAT CTGACAACGA AACTTCTGAA 
GCCCAGGAAG ATGATGAAGC TTCTGAAGCT CAGGAAGATG ATGAAGCTTC TGAAGCCCAG 
GAAGATGAGG AAGCTTCTGA ACTGGAAGCC CATGAGGAAG ATGATGATGA GGAAGAACTC 
TTGAAGCAAT CTGAAAGACA AAAAAGACAA ATGAGGCTGA AGAAGTACCT GGAGGATGAG 
GCTGAGGTGT CAGGGAGTGA AGTAGGAAGC GAGGATGAGT ATGATGGGGA GGAGATCGAT 
GAATACGAAG AAGATGTAAT TGATGAAGAG CTTCCCTCAG ATGAAGAACT GCAGGATCAG 
GTCAACAAAA TACACAGGAA AGCTATGTTG GATGATGACA AGCGACAATT ACGCTTATAC 
CAGGAGAGGT ACCTTGCTGA TGGAGACCTG CACAGTGATG GCCCTGGACG AATGAGGAAA 
TTCCGTTGGA AAAATATCGA TGATGCTTCC CAGATGGACA TGTTTCACAG AGACTCTGAT 
GAGGATCAGA ATGAAGAACA GTTTGATGAA ACAGAAGCCA AATGGAGGAA AGAGCGGATT 
GAGCGAGAAC AGTGGCTTAG AGACCAGGCT CAGCAGGGGA AAATTACAGC TGAAGAGGAT 
GATGAAATTG GAGAAGAGAG TCAGTTTATG ATGCTGGCTA AGAAAGCCAC TGCCAAAGCA 
CTTCAGAAGA AAGCCAACCA TGTCACTGGT GCTCAGGAAG CAAAAACCTT GCCCAGAAGT 
CCCTTTGAAG CCATCAGACC TGGAAGTGCC CACCAGCTGA AGACAGGCTC ACTGCTGAAT 
CAACCCAAAA CTCTGCTGCA GAAGTTATCT GCCCTCTCTG ACCTCAACCC TAGTGCTCCT 
CGAAACTCTA GGAATTTTGT CTTCCACACA CTCTCCCCAG TCAAGAATGA GATGGCAAAG 
GAGCCTGCTA AGCCTCAGGT GAAGAGAAAG GGCATACCTT TGGCCTCCCC ATCTCCCAAG 
CACTTCAAAG CAAATGGCAC TTTAGGATCA AACCGGAGCA TCTTTAAGTA CTTGGAAAGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CLSPN ( XM_031962132.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031817992.1 Sarcophilus harrisii claspin (CLSPN), mRNA.

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CloneID	OSa231203
Clone ID Related Accession (Same CDS sequence)	XM_031962132.1
Accession Version	XM_031962132.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4152bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	claspin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045428.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGAG AGGTGGTGAC TGAGCTTCAC CTAAATGTTG AAGATCCCAA ATTGAACACA 
AAGGAAGAAA TTGATTCAGA CAGTGGGCAG AGCAGCTGTG AAACAGGGTC TCCAGGACAT 
TCTAACACAA AGAACACACC CCTGATTGAA AGAGACTCAG ACGAAGAGAT CTTTGTGAGT 
AAGAAATTGA AACGTAACAA GTTTCTCCTG CAGGACAGTG ACAGTGAAGA AGAGGACAGA 
GATGTCCTTC CTAAGAAGTC TGACTATGAC AATGCAGAAC TGGAAAGTGG GGAGAATAAA 
GAAAACATAT ATGCAGGGAA AAAAGGGAAA GCACGAAAGA TTTATCAGAC TCTGAAAGAC 
AGTGATGAGA GTGACACTGA GGAGCTACCA TATCAGAAGA ACCTTGACAG CCGGATGATG 
ATTCCTTTGG CTGAAAGTAG CTTGGATCTG GGTTTGCAAT CCCCAGAGTC TGTGACCTTG 
CCAACAGGTG CTAGGAAAAG GTTCAAAGAA TGTACTCGAG CTAAAGAAGA TACTGAAGGG 
AAAACAAAAG GGAGGTCACA GAGAAGGCTT GAGAAAGAAG CCAAAAGGAT GGAAGTGATT 
AGTCGGCTGA AAAAAAAGGA AAATAAAAAT GAGGAAGATG AAACCAAACA ACCTTTCAAT 
GATAGTGGCT GTCTTCTTGG AGATAGTGAC CTTTTTGAGA CTGGTTTGGA GGAAGATCTC 
CCACCAGAAG ATGAAGAATC ATTAGAATCC ATACGAGCTT CTGTGAAAGG CAAAGTCAAA 
AAGCACAAGA AAAAGGAGCT GTCTTTGGAA AGTGGGGGCT GTTCATTTGA AGATGAAAAG 
GAGCTGTCAA AAGGAATGAT AAGAAAGGAG AGAAAGGCGG CCAAGCTAAG TAAGGAAGCT 
ATGAAAAGGC TACATAGTGA GAGCCAACGC CTTATTCGAG AGTCTGCCCT CTCTCTTCCC 
TATCACATGC CTGAAAACAA AACCGTTCAT GACTTCTTCA GAAGCAAGCC TCGACCTTCC 
GGCCAGGGAA ACGCCATGGC GCTGCTGAAG TCCTCTAAGT ATCAGGCAGC CTTCAGTAAA 
GAAATCAGCA CCACTGCTAG CTCAAGTGAC ATCAGCAGCA CCAGCCACTC CAGAGGCTCT 
AATCAAATGG CGACGGGCCC TGACCTGGAA ACTGAGAACA GCCCACTGCC CTTAGCTCCC 
CAGGCCCCTG CCAACTCTGC TGACCAGCCT GCTGGTGAGG CTGGCACAAG AGAGGAGTCA 
GGACGCTGTG GGGGTCGGGG GCAGAAGGAA CTCGTGGAGG CCCCTACTAG CCCTTCACCC 
AAGGACAGCT CAGAGTGTCA GGAGGAATCT GGCGTCTTCA GGAGTAAGGA GCCAGCACAG 
ATGGAATTGA GGGCCCCTGT GGCCCTTGAA CTCAATGTTC TAGAGGAAGA AGAAGAGCCT 
CAAGATCCTA ACAAAACAAA GAGCAAGGTA GATGACAGCA GGCAACAAGC TGTGGGAAAG 
GCCACTATTA CATCAGAAAA AGGAAGGAAG GTCAGCTTCC TGGATAAACT CCAGCGACTG 
GGAGTAGATC TCTCCATTAA GCCAAAGCTA GGGGCAGATG AAGATTCTTT TGTGACTCTT 
GACGAGCCTG AACCCAATAA AGAACTGGAA GCTTTAAAGG AACGTTTCTG GAAGCACGCA 
TGTCCCACAG CCAAGGCCCA AACTGTGTCT GAACGAAAAA TGAACCTGAA CATTATAGTG 
AAGGAGACTG GTGCGGATGG CAAGGAAGAG CTGAAGGCTG ATGTGGTCCC CCTGACTTTG 
GCTACAGAAA AATTAGATGG AATGAGCTAT GCAAAACCAG GTGAGAAGCT ACAGGTGCTG 
AAAGCAAAAC TCCAGGAAGC AATGAAACTC CGAAGACTTG AGGAGCGCCA GAAGCGGCAG 
GCGTTGTTCA AGCTGGATAA TGAGGATGGG TTTGAGGAAG AGGAAGAGGA GGAGGAGGAG 
GAGGAGATGA CAGATGAATC AGATAAGGAA GAGGAGGAGA AGGAAGAAGA AGAGGAGGAG 
GAGGAGGAGG AGGAAGGCAA TCAGGAGACA GGAGAATATT TTCTTGATGA TGAAGAGGTA 
GAAGACAAGG ATATGAAAGA GGAAGATAAA GAAATGGATA AGGAGAACAT TATTGACAGC 
ACAGAAATTT TCAAGGCTGT TGATTGTAGT TCTATTCCTA AGCCTCCCAC TTTGGAATCT 
ACCTTGCTCC TGTTCAAAGA CAGCTCTTCC AAAATGGGAT ACTATCCTGC TGAAGAAAAA 
TCTGAGACTG ATGAAAGTAT AGGCAAAAGA CCTAGTAAAC TAGAAGAAGA TGATTCATGC 
TCATTGCTAA CAAAGGAGAG CAGCCACAAC AGCAGCTTTG AGATGATCGG CTCCATGATT 
GCTCCCTATC AGCCTTGCAA CAAGCAGTCA GGTCGTGGGA CTAGTTTCCC CCCTGGGCTG 
GGGGGGTTCC GATCCCCCTC CCCTGGGTTA TTTCGAGCAA GTTTGATGAG CTCGACTTCC 
AAGAGTTCAG GGAAGCTGTC TGAGCCTTCC CTTCCCATAG AGGATTCCCA GGAGCTGTAT 
AACGCCTCCC CGGAGCCGAA AACTCTCTTC CTGGGAGCAG GAGACTTCCA GTTCTGTTTG 
GAAGATGACA CTCAGAGCCA ACTGTTAGAT GCAGATGGGT TCTTAAATGT TGGAAATCAT 
CGAAATAAAT ACCAGGCTTT GAAATCACGG GTGCCTTTGG CCAGTATGGA TGAGAATGCC 
ATGGATGCCA ACATGGATGA GCTGTTGGAT TTGTGTACAG GAAAGTTTAA GGATGAAAAA 
CCTTTGCCTG AGAAGGATGA CAGAAAGGAA GACATGGAGG AACTTTTGGG TCTTTGTTCA 
GGGAAATTCA CTTCTCAGGG TTCTTCAACT CCTGCTCCTT CAGAGTCCTC CAAACAGGAG 
AAGGACATGA ACACTGATGA CCCAATGGCA GAAGCCCTTG CTCTTTGTTC AGGGTCCTTC 
CCAACAGATA AGGAACTGGA AGGTGAAGAA GAAGAGGAGG AGTTTGGTGA TTTTCAGCTT 
GTCACTGATA GCAATGTGTT TGATAGTGAA GAAGAGGAGC ATTCTGAATC TGACAACGAA 
ACTTCTGAAG CCCAGGAAGA TGATGAAGCT TCTGAAGCTC AGGAAGATGA TGAAGCTTCT 
GAAGCCCAGG AAGATGAGGA AGCTTCTGAA CTGGAAGCCC ATGAGGAAGA TGATGATGAG 
GAAGAACTCT TGAAGCAATC TGAAAGACAA AAAAGACAAA TGAGGCTGAA GAAGTACCTG 
GAGGATGAGG CTGAGGTGTC AGGGAGTGAA GTAGGAAGCG AGGATGAGTA TGATGGGGAG 
GAGATCGATG AATACGAAGA AGATGTAATT GATGAAGAGC TTCCCTCAGA TGAAGAACTG 
CAGGATCAGG TCAACAAAAT ACACAGGAAA GCTATGTTGG ATGATGACAA GCGACAATTA 
CGCTTATACC AGGAGAGGTA CCTTGCTGAT GGAGACCTGC ACAGTGATGG CCCTGGACGA 
ATGAGGAAAT TCCGTTGGAA AAATATCGAT GATGCTTCCC AGATGGACAT GTTTCACAGA 
GACTCTGATG AGGATCAGAA TGAAGAACAG TTTGATGAAA CAGAAGCCAA ATGGAGGAAA 
GAGCGGATTG AGCGAGAACA GTGGCTTAGA GACCAGGCTC AGCAGGGGAA AATTACAGCT 
GAAGAGGATG ATGAAATTGG AGAAGAGAGT CAGTTTATGA TGCTGGCTAA GAAAGCCACT 
GCCAAAGCAC TTCAGAAGAA AGCCAACCAT GTCACTGGTG CTCAGGAAGC AAAAACCTTG 
CCCAGAAGTC CCTTTGAAGC CATCAGACCT GGAAGTGCCC ACCAGCTGAA GACAGGCTCA 
CTGCTGAATC AACCCAAAAC TCTGCTGCAG AAGTTATCTG CCCTCTCTGA CCTCAACCCT 
AGTGCTCCTC GAAACTCTAG GAATTTTGTC TTCCACACAC TCTCCCCAGT CAAGAATGAG 
ATGGCAAAGG AGCCTGCTAA GCCTCAGGTG AAGAGAAAGG GCATACCTTT GGCCTCCCCA 
TCTCCCAAGC ACTTCAAAGC AAATGGCACT TTAGGATCAA ACCGGAGCAT CTTTAAGTAC 
TTGGAAAGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031817992.1
CDS	191..4342
Translation	MAGEVVTELHLNVEDPKLNTKEEIDSDSGQSSCETGSPGHSNTKNTPLIERDSDEEIFVSKKLKRNKFLLQDSDSEEEDRDVLPKKSDYDNAELESGENKENIYAGKKGKARKIYQTLKDSDESDTEELPYQKNLDSRMMIPLAESSLDLGLQSPESVTLPTGARKRFKECTRAKEDTEGKTKGRSQRRLEKEAKRMEVISRLKKKENKNEEDETKQPFNDSGCLLGDSDLFETGLEEDLPPEDEESLESIRASVKGKVKKHKKKELSLESGGCSFEDEKELSKGMIRKERKAAKLSKEAMKRLHSESQRLIRESALSLPYHMPENKTVHDFFRSKPRPSGQGNAMALLKSSKYQAAFSKEISTTASSSDISSTSHSRGSNQMATGPDLETENSPLPLAPQAPANSADQPAGEAGTREESGRCGGRGQKELVEAPTSPSPKDSSECQEESGVFRSKEPAQMELRAPVALELNVLEEEEEPQDPNKTKSKVDDSRQQAVGKATITSEKGRKVSFLDKLQRLGVDLSIKPKLGADEDSFVTLDEPEPNKELEALKERFWKHACPTAKAQTVSERKMNLNIIVKETGADGKEELKADVVPLTLATEKLDGMSYAKPGEKLQVLKAKLQEAMKLRRLEERQKRQALFKLDNEDGFEEEEEEEEEEEMTDESDKEEEEKEEEEEEEEEEEGNQETGEYFLDDEEVEDKDMKEEDKEMDKENIIDSTEIFKAVDCSSIPKPPTLESTLLLFKDSSSKMGYYPAEEKSETDESIGKRPSKLEEDDSCSLLTKESSHNSSFEMIGSMIAPYQPCNKQSGRGTSFPPGLGGFRSPSPGLFRASLMSSTSKSSGKLSEPSLPIEDSQELYNASPEPKTLFLGAGDFQFCLEDDTQSQLLDADGFLNVGNHRNKYQALKSRVPLASMDENAMDANMDELLDLCTGKFKDEKPLPEKDDRKEDMEELLGLCSGKFTSQGSSTPAPSESSKQEKDMNTDDPMAEALALCSGSFPTDKELEGEEEEEEFGDFQLVTDSNVFDSEEEEHSESDNETSEAQEDDEASEAQEDDEASEAQEDEEASELEAHEEDDDEEELLKQSERQKRQMRLKKYLEDEAEVSGSEVGSEDEYDGEEIDEYEEDVIDEELPSDEELQDQVNKIHRKAMLDDDKRQLRLYQERYLADGDLHSDGPGRMRKFRWKNIDDASQMDMFHRDSDEDQNEEQFDETEAKWRKERIEREQWLRDQAQQGKITAEEDDEIGEESQFMMLAKKATAKALQKKANHVTGAQEAKTLPRSPFEAIRPGSAHQLKTGSLLNQPKTLLQKLSALSDLNPSAPRNSRNFVFHTLSPVKNEMAKEPAKPQVKRKGIPLASPSPKHFKANGTLGSNRSIFKYLES

Target ORF information:

RefSeq Version	XM_031962132.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii claspin (CLSPN), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031962132.1

ORF Insert Sequence:

ATGGCAGGAG AGGTGGTGAC TGAGCTTCAC CTAAATGTTG AAGATCCCAA ATTGAACACA 
AAGGAAGAAA TTGATTCAGA CAGTGGGCAG AGCAGCTGTG AAACAGGGTC TCCAGGACAT 
TCTAACACAA AGAACACACC CCTGATTGAA AGAGACTCAG ACGAAGAGAT CTTTGTGAGT 
AAGAAATTGA AACGTAACAA GTTTCTCCTG CAGGACAGTG ACAGTGAAGA AGAGGACAGA 
GATGTCCTTC CTAAGAAGTC TGACTATGAC AATGCAGAAC TGGAAAGTGG GGAGAATAAA 
GAAAACATAT ATGCAGGGAA AAAAGGGAAA GCACGAAAGA TTTATCAGAC TCTGAAAGAC 
AGTGATGAGA GTGACACTGA GGAGCTACCA TATCAGAAGA ACCTTGACAG CCGGATGATG 
ATTCCTTTGG CTGAAAGTAG CTTGGATCTG GGTTTGCAAT CCCCAGAGTC TGTGACCTTG 
CCAACAGGTG CTAGGAAAAG GTTCAAAGAA TGTACTCGAG CTAAAGAAGA TACTGAAGGG 
AAAACAAAAG GGAGGTCACA GAGAAGGCTT GAGAAAGAAG CCAAAAGGAT GGAAGTGATT 
AGTCGGCTGA AAAAAAAGGA AAATAAAAAT GAGGAAGATG AAACCAAACA ACCTTTCAAT 
GATAGTGGCT GTCTTCTTGG AGATAGTGAC CTTTTTGAGA CTGGTTTGGA GGAAGATCTC 
CCACCAGAAG ATGAAGAATC ATTAGAATCC ATACGAGCTT CTGTGAAAGG CAAAGTCAAA 
AAGCACAAGA AAAAGGAGCT GTCTTTGGAA AGTGGGGGCT GTTCATTTGA AGATGAAAAG 
GAGCTGTCAA AAGGAATGAT AAGAAAGGAG AGAAAGGCGG CCAAGCTAAG TAAGGAAGCT 
ATGAAAAGGC TACATAGTGA GAGCCAACGC CTTATTCGAG AGTCTGCCCT CTCTCTTCCC 
TATCACATGC CTGAAAACAA AACCGTTCAT GACTTCTTCA GAAGCAAGCC TCGACCTTCC 
GGCCAGGGAA ACGCCATGGC GCTGCTGAAG TCCTCTAAGT ATCAGGCAGC CTTCAGTAAA 
GAAATCAGCA CCACTGCTAG CTCAAGTGAC ATCAGCAGCA CCAGCCACTC CAGAGGCTCT 
AATCAAATGG CGACGGGCCC TGACCTGGAA ACTGAGAACA GCCCACTGCC CTTAGCTCCC 
CAGGCCCCTG CCAACTCTGC TGACCAGCCT GCTGGTGAGG CTGGCACAAG AGAGGAGTCA 
GGACGCTGTG GGGGTCGGGG GCAGAAGGAA CTCGTGGAGG CCCCTACTAG CCCTTCACCC 
AAGGACAGCT CAGAGTGTCA GGAGGAATCT GGCGTCTTCA GGAGTAAGGA GCCAGCACAG 
ATGGAATTGA GGGCCCCTGT GGCCCTTGAA CTCAATGTTC TAGAGGAAGA AGAAGAGCCT 
CAAGATCCTA ACAAAACAAA GAGCAAGGTA GATGACAGCA GGCAACAAGC TGTGGGAAAG 
GCCACTATTA CATCAGAAAA AGGAAGGAAG GTCAGCTTCC TGGATAAACT CCAGCGACTG 
GGAGTAGATC TCTCCATTAA GCCAAAGCTA GGGGCAGATG AAGATTCTTT TGTGACTCTT 
GACGAGCCTG AACCCAATAA AGAACTGGAA GCTTTAAAGG AACGTTTCTG GAAGCACGCA 
TGTCCCACAG CCAAGGCCCA AACTGTGTCT GAACGAAAAA TGAACCTGAA CATTATAGTG 
AAGGAGACTG GTGCGGATGG CAAGGAAGAG CTGAAGGCTG ATGTGGTCCC CCTGACTTTG 
GCTACAGAAA AATTAGATGG AATGAGCTAT GCAAAACCAG GTGAGAAGCT ACAGGTGCTG 
AAAGCAAAAC TCCAGGAAGC AATGAAACTC CGAAGACTTG AGGAGCGCCA GAAGCGGCAG 
GCGTTGTTCA AGCTGGATAA TGAGGATGGG TTTGAGGAAG AGGAAGAGGA GGAGGAGGAG 
GAGGAGATGA CAGATGAATC AGATAAGGAA GAGGAGGAGA AGGAAGAAGA AGAGGAGGAG 
GAGGAGGAGG AGGAAGGCAA TCAGGAGACA GGAGAATATT TTCTTGATGA TGAAGAGGTA 
GAAGACAAGG ATATGAAAGA GGAAGATAAA GAAATGGATA AGGAGAACAT TATTGACAGC 
ACAGAAATTT TCAAGGCTGT TGATTGTAGT TCTATTCCTA AGCCTCCCAC TTTGGAATCT 
ACCTTGCTCC TGTTCAAAGA CAGCTCTTCC AAAATGGGAT ACTATCCTGC TGAAGAAAAA 
TCTGAGACTG ATGAAAGTAT AGGCAAAAGA CCTAGTAAAC TAGAAGAAGA TGATTCATGC 
TCATTGCTAA CAAAGGAGAG CAGCCACAAC AGCAGCTTTG AGATGATCGG CTCCATGATT 
GCTCCCTATC AGCCTTGCAA CAAGCAGTCA GGTCGTGGGA CTAGTTTCCC CCCTGGGCTG 
GGGGGGTTCC GATCCCCCTC CCCTGGGTTA TTTCGAGCAA GTTTGATGAG CTCGACTTCC 
AAGAGTTCAG GGAAGCTGTC TGAGCCTTCC CTTCCCATAG AGGATTCCCA GGAGCTGTAT 
AACGCCTCCC CGGAGCCGAA AACTCTCTTC CTGGGAGCAG GAGACTTCCA GTTCTGTTTG 
GAAGATGACA CTCAGAGCCA ACTGTTAGAT GCAGATGGGT TCTTAAATGT TGGAAATCAT 
CGAAATAAAT ACCAGGCTTT GAAATCACGG GTGCCTTTGG CCAGTATGGA TGAGAATGCC 
ATGGATGCCA ACATGGATGA GCTGTTGGAT TTGTGTACAG GAAAGTTTAA GGATGAAAAA 
CCTTTGCCTG AGAAGGATGA CAGAAAGGAA GACATGGAGG AACTTTTGGG TCTTTGTTCA 
GGGAAATTCA CTTCTCAGGG TTCTTCAACT CCTGCTCCTT CAGAGTCCTC CAAACAGGAG 
AAGGACATGA ACACTGATGA CCCAATGGCA GAAGCCCTTG CTCTTTGTTC AGGGTCCTTC 
CCAACAGATA AGGAACTGGA AGGTGAAGAA GAAGAGGAGG AGTTTGGTGA TTTTCAGCTT 
GTCACTGATA GCAATGTGTT TGATAGTGAA GAAGAGGAGC ATTCTGAATC TGACAACGAA 
ACTTCTGAAG CCCAGGAAGA TGATGAAGCT TCTGAAGCTC AGGAAGATGA TGAAGCTTCT 
GAAGCCCAGG AAGATGAGGA AGCTTCTGAA CTGGAAGCCC ATGAGGAAGA TGATGATGAG 
GAAGAACTCT TGAAGCAATC TGAAAGACAA AAAAGACAAA TGAGGCTGAA GAAGTACCTG 
GAGGATGAGG CTGAGGTGTC AGGGAGTGAA GTAGGAAGCG AGGATGAGTA TGATGGGGAG 
GAGATCGATG AATACGAAGA AGATGTAATT GATGAAGAGC TTCCCTCAGA TGAAGAACTG 
CAGGATCAGG TCAACAAAAT ACACAGGAAA GCTATGTTGG ATGATGACAA GCGACAATTA 
CGCTTATACC AGGAGAGGTA CCTTGCTGAT GGAGACCTGC ACAGTGATGG CCCTGGACGA 
ATGAGGAAAT TCCGTTGGAA AAATATCGAT GATGCTTCCC AGATGGACAT GTTTCACAGA 
GACTCTGATG AGGATCAGAA TGAAGAACAG TTTGATGAAA CAGAAGCCAA ATGGAGGAAA 
GAGCGGATTG AGCGAGAACA GTGGCTTAGA GACCAGGCTC AGCAGGGGAA AATTACAGCT 
GAAGAGGATG ATGAAATTGG AGAAGAGAGT CAGTTTATGA TGCTGGCTAA GAAAGCCACT 
GCCAAAGCAC TTCAGAAGAA AGCCAACCAT GTCACTGGTG CTCAGGAAGC AAAAACCTTG 
CCCAGAAGTC CCTTTGAAGC CATCAGACCT GGAAGTGCCC ACCAGCTGAA GACAGGCTCA 
CTGCTGAATC AACCCAAAAC TCTGCTGCAG AAGTTATCTG CCCTCTCTGA CCTCAACCCT 
AGTGCTCCTC GAAACTCTAG GAATTTTGTC TTCCACACAC TCTCCCCAGT CAAGAATGAG 
ATGGCAAAGG AGCCTGCTAA GCCTCAGGTG AAGAGAAAGG GCATACCTTT GGCCTCCCCA 
TCTCCCAAGC ACTTCAAAGC AAATGGCACT TTAGGATCAA ACCGGAGCAT CTTTAAGTAC 
TTGGAAAGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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CLSPN cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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