ROCK1 cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene
Clones
gRNAs

Summary

Gene Symbol	ROCK1
Entrez Gene ID	100916611
Full Name	Rho associated coiled-coil containing protein kinase 1
Gene Type	protein-coding
Organism	Sarcophilus harrisii(Tasmanian devil)

mRNA and Protein(s)

mRNA	Protein	Name
XM_031946551.1	XP_031802411.1	rho-associated protein kinase 1 isoform X1
XM_003760018.2	XP_003760066.1	rho-associated protein kinase 1
XM_023496213.1	XP_023351981.1	rho-associated protein kinase 1
XM_031946552.1	XP_031802412.1	rho-associated protein kinase 1 isoform X2

Pathways from BioSystems
Gene Ontology

The following ROCK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ROCK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSa121669	XM_003760018.2 Latest version!	Sarcophilus harrisii Rho-associated, coiled-coil containing protein kinase 1 (ROCK1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OSa158628	XM_023496213.1 Latest version!	Sarcophilus harrisii Rho associated coiled-coil containing protein kinase 1 (ROCK1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OSa158628	XM_031946551.1 Latest version!	Sarcophilus harrisii Rho associated coiled-coil containing protein kinase 1 (ROCK1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OSa221368	XM_031946552.1 Latest version!	Sarcophilus harrisii Rho associated coiled-coil containing protein kinase 1 (ROCK1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ROCK1 ( XM_003760018.2 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_003760066.1 Sarcophilus harrisii Rho-associated, coiled-coil containing protein kinase 1 (ROCK1), mRNA.

Price & Availability↑

CloneID	OSa121669
Clone ID Related Accession (Same CDS sequence)	XM_003760018.2
Accession Version	XM_003760018.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3507bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1431532800000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	rho-associated protein kinase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003823314.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 15, 2015 this sequence version replaced XM_003760018.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sarcophilus harrisii Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGTCGACTG GGGACAGTTT TGAGACTCGG TTTGAGAAAA TCGACAACCT GCTGCGGGAC 
CCGAAGTCGG AAGTGAATTC GGATTGTTTG CTGGATGGAT TGGATGCTTT GGTATATGAT 
TTGGATTTTC CTGCACTAAG AAAAAACAAA AATATTGACA ACTTTTTAAG CAAATATAAA 
GATACAATAA ATAAAATTAG AGATTTGCGC ATGAAAGCTG AAGATTATGA AGTGGTGAAA 
GTGATTGGTA GAGGTGCATT TGGAGAAGTG CAATTGGTAA GGCATAAATC TACCAGGAAG 
GTATATGCTA TGAAGCTGCT GAGCAAATTT GAGATGATTA AGAGATCAGA TTCTGCTTTC 
TTCTGGGAAG AACGGGACAT TATGGCTTTT GCTAATAGCC CTTGGGTCGT TCAGCTATAT 
TATGCATTCC AAGATGATCG GTATCTCTAC ATGGTAATGG AATATATGCC TGGTGGGGAT 
CTTGTTAATT TAATGAGCAA CTATGATGTG CCTGAAAAAT GGGCCCGTTT TTATACTGCG 
GAAGTAGTTC TTGCTTTGGA TGCAATCCAT TCAATGGGCT TTATTCATAG AGATGTGAAG 
CCTGATAACA TGCTGCTGGA TAAATCTGGT CACTTGAAGC TAGCTGATTT TGGTACTTGT 
ATGAAGATGA ATAAAGAAGG TATGGTACGA TGTGATACAG CAGTTGGAAC ACCTGACTAT 
ATTTCTCCTG AAGTATTAAA ATCCCAAGGT GGTGATGGCT ATTATGGGCG AGAATGTGAC 
TGGTGGTCTG TTGGTGTGTT TTTATATGAA ATGCTTGTAG GTGATACACC ATTTTATGCA 
GATTCACTGG TTGGAACATA TAGCAAAATT ATGAACCATA AGAATTCTCT TACCTTTCCT 
GATGATAATG ATATATCAAA GGAAGCAAAA AATCTTATTT GTGCCTTCCT TACAGACAGG 
GAAGTGAGAT TAGGACGAAA TGGTGTGGAA GAAATCAAAC GACATCTCTT CTTCAAAAAT 
GATCAATGGG CATGGGAAAC TCTTCGAGAT ACTGTTGCAC CTGTTGTACC TGATCTCAGT 
AGTGACATTG ATACTAGTAA TTTTGATGAC TTAGAAGAAG ACAAAGGAGA AGAAGAAACA 
TTTCCCATTC CAAAAGCTTT TGTTGGCAAT CAACTACCTT TTGTAGGATT TACATATTAT 
AGCAATCGTA GATATTTATC TGTATCTTCA GCCAATCCTA ATGATAATAG AACCACCTCC 
AATATAGATA AAAGCTTGCA GGAAAGTTTG CAAAATACAA TCTATAAGCT GGAAGAACAA 
TTGCATAATG AAATACAGCT AAAAGATGAA ATGGAGCAGA AGTGCAGAAC TTCAAACATA 
AAACTAGACA AGATAATGAA AGAACTGGAT GAAGAGGGAA ATCAAAGAAA AAATTTAGAA 
TCCACAGTGT CTCAGATTGA GAAAGAGAAA ATGTTGCTGC AGCACAGAGT TAATGAGTAC 
CAAAGAAAAA TAGAACAGGA GAATGAGAAG AGAAGGAATG TTGAAAATGA AGTTTCTACA 
TTAAAAGACC AATTGGATGA CCTGAAGAAA GTTAGTCAGA ATTCTCAGCT TACAAATGAG 
AAAATGGCAC AATTACAGAA GCAACTAAAA GAAGCGAATG ACTTACTGAG GACAGAATCA 
GACACAGCAG CAAGATTAAG AAAGAGCCAC ACAGAATTAA GCAAATCAGT AAGTCAGTTA 
GAGTCCCTAA ACAGAGAATT GCAAGAGCGA AACAGAATTT TAGAGACAAC CAAATCACAA 
GTGGACAAGG ATTATTACCA GCTTCAGGCT GCATTAGAAG CTGAAAGAAG AGACCGAAGT 
CATGGATCGG AGATGATAGG AGATCTTCAA GGTAAAATAT TCAAGAAAAG TAATTTGGTG 
AAACCAGGAG TACAATATTG TGGAAACGAA GGACAGATGC GGGAACTACA GGATCAGCTT 
GAAGCTGAAC AATATTTTTC AACACTTTAT AAAACTCAGG TAAAGGAACT TAAAGAGGAC 
ATTGAAGAAA AAAATCGAGA AAATCTAAAG AAAATACAAG AGCTTCAAAA TGAAAAAGAA 
ACACTTGCTG CACAGTTAGA CCTGGTAGAA ACAAAAGCTG AATCAGAGCA GTTGGCTCGA 
CGTCTACTGG AAGAACAATA TTTTGAATTG ACTCAAGAGT GTAAAAAAGC AGCTTCACGA 
AATAAACAAG AAATTACAGA TAAAGATCAC ACTGTCAATC GGCTGGAAGA AATAAACAGT 
ACATTAACCA AAGATATTGA ATTATTAAGA AAGGAAAATG AAGAGTTTAC TGAAAAAATG 
AGGAAGGAAC AAGAAGATTA TAAATTGAAG AAAGAAGAAG AGATCAATAA TCTTAAAGCT 
TCTTTTGAAA AGAATATTAA TACTGAAAGA ACTCTAAAAA CGCAGGCTGT TAACAAATTG 
GCTGAAATAA TGAATCGTAA AGATTTTAAA ATTGATAGAA AGAAGGCTAA TACACAGGAT 
TTAAGAAAGA AGGAAAAGGA AAACAGGAAG TTACAACTGG AACTCAACCA AGAAAGAGAG 
AAATTCAATC AAATGGTAGT GAAACACCAG AAAGAACTCA ATGAAATGCA AGCGCAATTG 
GTTGAAGAGT GCACACACAG GAATGAACTT CAGATGCAGC TGGCTAGCAA AGAGAGTGAC 
ATAGAGCAGT TGCGTGCAAA ACTTTTGGAC CTTTCAGATT CTACCAGTGT TACTAGTTTT 
CCTAGTGCCG ATGAAACTGA TGGAAACCTT CCAGAATCAA GAATTGAAGG TTGGCTATCG 
GTTCCAAACA GAGGAAATAT AAAACGATAT GGCTGGAAGA AACAGTATGT CGTAGTAAGC 
AGCAAAAAAA TTTTGTTCTA CAATGATGAA CAGGATAAGG AACAGTCAAA TCCATCTATG 
GTACTAGACA TAGACAAACT ATTCCATGTT CGACCTGTAA CCCAAGGTGA TGTGTACAGA 
GCAGAAACCG AAGAAATCCC TAAAATATTC CAGATATTGT ATGCAAATGA AGGTGAATGT 
AGGAAAGATT TGGAAATGGA GCCAGTACAA CAAGCTGAGA AGACCAATTT TCAGAATCAC 
AAAGGCCATG AGTTTATTCC TACACTCTAT CACTTTCCAG CCAACTGTGA GGCTTGTGCC 
AAACCTCTCT GGCATGTTTT TAAGCCACCC CCTGCCCTCG AATGTCGAAG ATGCCATGTC 
AAGTGCCATA GAGATCACTT AGACAAAAAA GAAGACTTAA TTTCTCCATG CAAAGTGAGT 
TATGATGTAA CATCAGCAAG AGATATGCTG CTGTTAGCAT GTTCTCAGGA TGAACAGAAG 
AAATGGGTAA CCCATTTAGT AAAGAAGATC CCTAAGAATC CACCATCCAG TTTTGTTCGT 
GCCTCCCCTA GAACACTTTC TACTAGATCT AGTGCAAATC AGTCTTTCCG GAAAGTTGTC 
AAAAATACAT CTGGGAAAAC TAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003760066.1
CDS	169..3675
Translation	MSTGDSFETRFEKIDNLLRDPKSEVNSDCLLDGLDALVYDLDFPALRKNKNIDNFLSKYKDTINKIRDLRMKAEDYEVVKVIGRGAFGEVQLVRHKSTRKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLYYAFQDDRYLYMVMEYMPGGDLVNLMSNYDVPEKWARFYTAEVVLALDAIHSMGFIHRDVKPDNMLLDKSGHLKLADFGTCMKMNKEGMVRCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLYEMLVGDTPFYADSLVGTYSKIMNHKNSLTFPDDNDISKEAKNLICAFLTDREVRLGRNGVEEIKRHLFFKNDQWAWETLRDTVAPVVPDLSSDIDTSNFDDLEEDKGEEETFPIPKAFVGNQLPFVGFTYYSNRRYLSVSSANPNDNRTTSNIDKSLQESLQNTIYKLEEQLHNEIQLKDEMEQKCRTSNIKLDKIMKELDEEGNQRKNLESTVSQIEKEKMLLQHRVNEYQRKIEQENEKRRNVENEVSTLKDQLDDLKKVSQNSQLTNEKMAQLQKQLKEANDLLRTESDTAARLRKSHTELSKSVSQLESLNRELQERNRILETTKSQVDKDYYQLQAALEAERRDRSHGSEMIGDLQGKIFKKSNLVKPGVQYCGNEGQMRELQDQLEAEQYFSTLYKTQVKELKEDIEEKNRENLKKIQELQNEKETLAAQLDLVETKAESEQLARRLLEEQYFELTQECKKAASRNKQEITDKDHTVNRLEEINSTLTKDIELLRKENEEFTEKMRKEQEDYKLKKEEEINNLKASFEKNINTERTLKTQAVNKLAEIMNRKDFKIDRKKANTQDLRKKEKENRKLQLELNQEREKFNQMVVKHQKELNEMQAQLVEECTHRNELQMQLASKESDIEQLRAKLLDLSDSTSVTSFPSADETDGNLPESRIEGWLSVPNRGNIKRYGWKKQYVVVSSKKILFYNDEQDKEQSNPSMVLDIDKLFHVRPVTQGDVYRAETEEIPKIFQILYANEGECRKDLEMEPVQQAEKTNFQNHKGHEFIPTLYHFPANCEACAKPLWHVFKPPPALECRRCHVKCHRDHLDKKEDLISPCKVSYDVTSARDMLLLACSQDEQKKWVTHLVKKIPKNPPSSFVRASPRTLSTRSSANQSFRKVVKNTSGKTS

Target ORF information:

RefSeq Version	XM_003760018.2
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii Rho-associated, coiled-coil containing protein kinase 1 (ROCK1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003760018.2

ORF Insert Sequence:

ATGTCGACTG GGGACAGTTT TGAGACTCGG TTTGAGAAAA TCGACAACCT GCTGCGGGAC 
CCGAAGTCGG AAGTGAATTC GGATTGTTTG CTGGATGGAT TGGATGCTTT GGTATATGAT 
TTGGATTTTC CTGCACTAAG AAAAAACAAA AATATTGACA ACTTTTTAAG CAAATATAAA 
GATACAATAA ATAAAATTAG AGATTTGCGC ATGAAAGCTG AAGATTATGA AGTGGTGAAA 
GTGATTGGTA GAGGTGCATT TGGAGAAGTG CAATTGGTAA GGCATAAATC TACCAGGAAG 
GTATATGCTA TGAAGCTGCT GAGCAAATTT GAGATGATTA AGAGATCAGA TTCTGCTTTC 
TTCTGGGAAG AACGGGACAT TATGGCTTTT GCTAATAGCC CTTGGGTCGT TCAGCTATAT 
TATGCATTCC AAGATGATCG GTATCTCTAC ATGGTAATGG AATATATGCC TGGTGGGGAT 
CTTGTTAATT TAATGAGCAA CTATGATGTG CCTGAAAAAT GGGCCCGTTT TTATACTGCG 
GAAGTAGTTC TTGCTTTGGA TGCAATCCAT TCAATGGGCT TTATTCATAG AGATGTGAAG 
CCTGATAACA TGCTGCTGGA TAAATCTGGT CACTTGAAGC TAGCTGATTT TGGTACTTGT 
ATGAAGATGA ATAAAGAAGG TATGGTACGA TGTGATACAG CAGTTGGAAC ACCTGACTAT 
ATTTCTCCTG AAGTATTAAA ATCCCAAGGT GGTGATGGCT ATTATGGGCG AGAATGTGAC 
TGGTGGTCTG TTGGTGTGTT TTTATATGAA ATGCTTGTAG GTGATACACC ATTTTATGCA 
GATTCACTGG TTGGAACATA TAGCAAAATT ATGAACCATA AGAATTCTCT TACCTTTCCT 
GATGATAATG ATATATCAAA GGAAGCAAAA AATCTTATTT GTGCCTTCCT TACAGACAGG 
GAAGTGAGAT TAGGACGAAA TGGTGTGGAA GAAATCAAAC GACATCTCTT CTTCAAAAAT 
GATCAATGGG CATGGGAAAC TCTTCGAGAT ACTGTTGCAC CTGTTGTACC TGATCTCAGT 
AGTGACATTG ATACTAGTAA TTTTGATGAC TTAGAAGAAG ACAAAGGAGA AGAAGAAACA 
TTTCCCATTC CAAAAGCTTT TGTTGGCAAT CAACTACCTT TTGTAGGATT TACATATTAT 
AGCAATCGTA GATATTTATC TGTATCTTCA GCCAATCCTA ATGATAATAG AACCACCTCC 
AATATAGATA AAAGCTTGCA GGAAAGTTTG CAAAATACAA TCTATAAGCT GGAAGAACAA 
TTGCATAATG AAATACAGCT AAAAGATGAA ATGGAGCAGA AGTGCAGAAC TTCAAACATA 
AAACTAGACA AGATAATGAA AGAACTGGAT GAAGAGGGAA ATCAAAGAAA AAATTTAGAA 
TCCACAGTGT CTCAGATTGA GAAAGAGAAA ATGTTGCTGC AGCACAGAGT TAATGAGTAC 
CAAAGAAAAA TAGAACAGGA GAATGAGAAG AGAAGGAATG TTGAAAATGA AGTTTCTACA 
TTAAAAGACC AATTGGATGA CCTGAAGAAA GTTAGTCAGA ATTCTCAGCT TACAAATGAG 
AAAATGGCAC AATTACAGAA GCAACTAAAA GAAGCGAATG ACTTACTGAG GACAGAATCA 
GACACAGCAG CAAGATTAAG AAAGAGCCAC ACAGAATTAA GCAAATCAGT AAGTCAGTTA 
GAGTCCCTAA ACAGAGAATT GCAAGAGCGA AACAGAATTT TAGAGACAAC CAAATCACAA 
GTGGACAAGG ATTATTACCA GCTTCAGGCT GCATTAGAAG CTGAAAGAAG AGACCGAAGT 
CATGGATCGG AGATGATAGG AGATCTTCAA GGTAAAATAT TCAAGAAAAG TAATTTGGTG 
AAACCAGGAG TACAATATTG TGGAAACGAA GGACAGATGC GGGAACTACA GGATCAGCTT 
GAAGCTGAAC AATATTTTTC AACACTTTAT AAAACTCAGG TAAAGGAACT TAAAGAGGAC 
ATTGAAGAAA AAAATCGAGA AAATCTAAAG AAAATACAAG AGCTTCAAAA TGAAAAAGAA 
ACACTTGCTG CACAGTTAGA CCTGGTAGAA ACAAAAGCTG AATCAGAGCA GTTGGCTCGA 
CGTCTACTGG AAGAACAATA TTTTGAATTG ACTCAAGAGT GTAAAAAAGC AGCTTCACGA 
AATAAACAAG AAATTACAGA TAAAGATCAC ACTGTCAATC GGCTGGAAGA AATAAACAGT 
ACATTAACCA AAGATATTGA ATTATTAAGA AAGGAAAATG AAGAGTTTAC TGAAAAAATG 
AGGAAGGAAC AAGAAGATTA TAAATTGAAG AAAGAAGAAG AGATCAATAA TCTTAAAGCT 
TCTTTTGAAA AGAATATTAA TACTGAAAGA ACTCTAAAAA CGCAGGCTGT TAACAAATTG 
GCTGAAATAA TGAATCGTAA AGATTTTAAA ATTGATAGAA AGAAGGCTAA TACACAGGAT 
TTAAGAAAGA AGGAAAAGGA AAACAGGAAG TTACAACTGG AACTCAACCA AGAAAGAGAG 
AAATTCAATC AAATGGTAGT GAAACACCAG AAAGAACTCA ATGAAATGCA AGCGCAATTG 
GTTGAAGAGT GCACACACAG GAATGAACTT CAGATGCAGC TGGCTAGCAA AGAGAGTGAC 
ATAGAGCAGT TGCGTGCAAA ACTTTTGGAC CTTTCAGATT CTACCAGTGT TACTAGTTTT 
CCTAGTGCCG ATGAAACTGA TGGAAACCTT CCAGAATCAA GAATTGAAGG TTGGCTATCG 
GTTCCAAACA GAGGAAATAT AAAACGATAT GGCTGGAAGA AACAGTATGT CGTAGTAAGC 
AGCAAAAAAA TTTTGTTCTA CAATGATGAA CAGGATAAGG AACAGTCAAA TCCATCTATG 
GTACTAGACA TAGACAAACT ATTCCATGTT CGACCTGTAA CCCAAGGTGA TGTGTACAGA 
GCAGAAACCG AAGAAATCCC TAAAATATTC CAGATATTGT ATGCAAATGA AGGTGAATGT 
AGGAAAGATT TGGAAATGGA GCCAGTACAA CAAGCTGAGA AGACCAATTT TCAGAATCAC 
AAAGGCCATG AGTTTATTCC TACACTCTAT CACTTTCCAG CCAACTGTGA GGCTTGTGCC 
AAACCTCTCT GGCATGTTTT TAAGCCACCC CCTGCCCTCG AATGTCGAAG ATGCCATGTC 
AAGTGCCATA GAGATCACTT AGACAAAAAA GAAGACTTAA TTTCTCCATG CAAAGTGAGT 
TATGATGTAA CATCAGCAAG AGATATGCTG CTGTTAGCAT GTTCTCAGGA TGAACAGAAG 
AAATGGGTAA CCCATTTAGT AAAGAAGATC CCTAAGAATC CACCATCCAG TTTTGTTCGT 
GCCTCCCCTA GAACACTTTC TACTAGATCT AGTGCAAATC AGTCTTTCCG GAAAGTTGTC 
AAAAATACAT CTGGGAAAAC TAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROCK1 ( XM_031946551.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031802411.1 Sarcophilus harrisii Rho associated coiled-coil containing protein kinase 1 (ROCK1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSa158628
Clone ID Related Accession (Same CDS sequence)	XM_031946551.1 , XM_023496213.1
Accession Version	XM_031946551.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4071bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	rho-associated protein kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045426.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGACTG GGGACAGTTT TGAGACTCGG TTTGAGAAAA TCGACAACCT GCTGCGGGAC 
CCGAAGTCGG AAGTGAATTC GGATTGTTTG CTGGATGGAT TGGATGCTTT GGTATATGAT 
TTGGATTTTC CTGCACTAAG AAAAAACAAA AATATTGACA ACTTTTTAAG CAAATATAAA 
GATACAATAA ATAAAATTAG AGATTTGCGC ATGAAAGCTG AAGATTATGA AGTGGTGAAA 
GTGATTGGTA GAGGTGCATT TGGAGAAGTG CAATTGGTAA GGCATAAATC TACCAGGAAG 
GTATATGCTA TGAAGCTGCT GAGCAAATTT GAGATGATTA AGAGATCAGA TTCTGCTTTC 
TTCTGGGAAG AACGGGACAT TATGGCTTTT GCTAATAGCC CTTGGGTCGT TCAGCTATAT 
TATGCATTCC AAGATGATCG GTATCTCTAC ATGGTAATGG AATATATGCC TGGTGGGGAT 
CTTGTTAATT TAATGAGCAA CTATGATGTG CCTGAAAAAT GGGCCCGTTT TTATACTGCG 
GAAGTAGTTC TTGCTTTGGA TGCAATCCAT TCAATGGGCT TTATTCATAG AGATGTGAAG 
CCTGATAACA TGCTGCTGGA TAAATCTGGT CACTTGAAGC TAGCTGATTT TGGTACTTGT 
ATGAAGATGA ATAAAGAAGG TATGGTACGA TGTGATACAG CAGTTGGAAC ACCTGACTAT 
ATTTCTCCTG AAGTATTAAA ATCCCAAGGT GGTGATGGCT ATTATGGGCG AGAATGTGAC 
TGGTGGTCTG TTGGTGTGTT TTTATATGAA ATGCTTGTAG GTGATACACC ATTTTATGCA 
GATTCACTGG TTGGAACATA TAGCAAAATT ATGAACCATA AGAATTCTCT TACCTTTCCT 
GATGATAATG ATATATCAAA GGAAGCAAAA AATCTTATTT GTGCCTTCCT TACAGACAGG 
GAAGTGAGAT TAGGACGAAA TGGTGTGGAA GAAATCAAAC GACATCTCTT CTTCAAAAAT 
GATCAATGGG CATGGGAAAC TCTTCGAGAT ACTGTTGCAC CTGTTGTACC TGATCTCAGT 
AGTGACATTG ATACTAGTAA TTTTGATGAC TTAGAAGAAG ACAAAGGAGA AGAAGAAACA 
TTTCCCATTC CAAAAGCTTT TGTTGGCAAT CAACTACCTT TTGTAGGATT TACATATTAT 
AGCAATCGTA GATATTTATC TGTATCTTCA GCCAATCCTA ATGATAATAG AACCACCTCC 
AATATAGATA AAAGCTTGCA GGAAAGTTTG CAAAATACAA TCTATAAGCT GGAAGAACAA 
TTGCATAATG AAATACAGCT AAAAGATGAA ATGGAGCAGA AGTGCAGAAC TTCAAACATA 
AAACTAGACA AGATAATGAA AGAACTGGAT GAAGAGGGAA ATCAAAGAAA AAATTTAGAA 
TCCACAGTGT CTCAGATTGA GAAAGAGAAA ATGTTGCTGC AGCACAGAGT TAATGAGTAC 
CAAAGAAAAA TAGAACAGGA GAATGAGAAG AGAAGGAATG TTGAAAATGA AGTTTCTACA 
TTAAAAGACC AATTGGATGA CCTGAAGAAA GTTAGTCAGA ATTCTCAGCT TACAAATGAG 
AAAATGGCAC AATTACAGAA GCAACTAAAA GAAGCGAATG ACTTACTGAG GACAGAATCA 
GACACAGCAG CAAGATTAAG AAAGAGCCAC ACAGAATTAA GCAAATCAGT AAGTCAGTTA 
GAGTCCCTAA ACAGAGAATT GCAAGAGCGA AACAGAATTT TAGAGACAAC CAAATCACAA 
GTGGACAAGG ATTATTACCA GCTTCAGGCT GCATTAGAAG CTGAAAGAAG AGACCGAAGT 
CATGGATCGG AGATGATAGG AGATCTTCAA GCTCGAATTA CATCTTTACA AGAAGAAGTG 
AAACACCTTA AACAAAATCT TGAAAGAGTG GAAGGGGAAA GAAAAGAAGC TCAAGACATG 
CTTAATCACT CAGAAAAGGA GAAGAATAAT TTGGAAATAG ATTTAAACTA CAAGCTAAAA 
TCATTACAAC AAAGATTAGA ACAGGAAGTA AATGAGCATA AAGTGACTAA AGCCCGTTTG 
ACTGACAAAC ATCAATCTAT TGAAGAAGCA AAGTCAGTTG CAATGTGTGA AATGGAAAAG 
AAACTGAAGG AAGAGAGAGC AGCCCGAGAG AAAGCTGAAA ATAGAGTTGT TCATATTGAA 
AAGCAGTGTT CTATGCTAGA TGTTGACTTG AAACAGTCTC AACAGAAACT GGAACATTTA 
GCAGAACATA AAGAAAGGAT GGAAGATGAA GTTAAGAATC TAACACTGCA GTTGGAGCAA 
GAATCAAATA AACGGTTGCT GATACAGAAT GAATTAAAAA CTCAAGCTTT TGAGAAGGAT 
AATTTGAAAG GGTTAGAAAA GCAAATGAAA CAAGAAATCA ATACATTATT GGAAGCAAAG 
AGATTACTGG AATTTGAACT AGCCCAGCTT ACAAGACAAT ATTGTGGAAA CGAAGGACAG 
ATGCGGGAAC TACAGGATCA GCTTGAAGCT GAACAATATT TTTCAACACT TTATAAAACT 
CAGGTAAAGG AACTTAAAGA GGACATTGAA GAAAAAAATC GAGAAAATCT AAAGAAAATA 
CAAGAGCTTC AAAATGAAAA AGAAACACTT GCTGCACAGT TAGACCTGGT AGAAACAAAA 
GCTGAATCAG AGCAGTTGGC TCGACGTCTA CTGGAAGAAC AATATTTTGA ATTGACTCAA 
GAGTGTAAAA AAGCAGCTTC ACGAAATAAA CAAGAAATTA CAGATAAAGA TCACACTGTC 
AATCGGCTGG AAGAAATAAA CAGTACATTA ACCAAAGATA TTGAATTATT AAGAAAGGAA 
AATGAAGAGT TTACTGAAAA AATGAGGAAG GAACAAGAAG ATTATAAATT GAAGAAAGAA 
GAAGAGATCA ATAATCTTAA AGCTTCTTTT GAAAAGAATA TTAATACTGA AAGAACTCTA 
AAAACGCAGG CTGTTAACAA ATTGGCTGAA ATAATGAATC GTAAAGATTT TAAAATTGAT 
AGAAAGAAGG CTAATACACA GGATTTAAGA AAGAAGGAAA AGGAAAACAG GAAGTTACAA 
CTGGAACTCA ACCAAGAAAG AGAGAAATTC AATCAAATGG TAGTGAAACA CCAGAAAGAA 
CTCAATGAAA TGCAAGCGCA ATTGGTTGAA GAGTGCACAC ACAGGAATGA ACTTCAGATG 
CAGCTGGCTA GCAAAGAGAG TGACATAGAG CAGTTGCGTG CAAAACTTTT GGACCTTTCA 
GATTCTACCA GTGTTACTAG TTTTCCTAGT GCCGATGAAA CTGATGGAAA CCTTCCAGAA 
TCAAGAATTG AAGGTTGGCT ATCGGTTCCA AACAGAGGAA ATATAAAACG ATATGGCTGG 
AAGAAACAGT ATGTCGTAGT AAGCAGCAAA AAAATTTTGT TCTACAATGA TGAACAGGAT 
AAGGAACAGT CAAATCCATC TATGGTACTA GACATAGACA AACTATTCCA TGTTCGACCT 
GTAACCCAAG GTGATGTGTA CAGAGCAGAA ACCGAAGAAA TCCCTAAAAT ATTCCAGATA 
TTGTATGCAA ATGAAGGTGA ATGTAGGAAA GATTTGGAAA TGGAGCCAGT ACAACAAGCT 
GAGAAGACCA ATTTTCAGAA TCACAAAGGC CATGAGTTTA TTCCTACACT CTATCACTTT 
CCAGCCAACT GTGAGGCTTG TGCCAAACCT CTCTGGCATG TTTTTAAGCC ACCCCCTGCC 
CTCGAATGTC GAAGATGCCA TGTCAAGTGC CATAGAGATC ACTTAGACAA AAAAGAAGAC 
TTAATTTCTC CATGCAAAGT GAGTTATGAT GTAACATCAG CAAGAGATAT GCTGCTGTTA 
GCATGTTCTC AGGATGAACA GAAGAAATGG GTAACCCATT TAGTAAAGAA GATCCCTAAG 
AATCCACCAT CCAGTTTTGT TCGTGCCTCC CCTAGAACAC TTTCTACTAG ATCTAGTGCA 
AATCAGTCTT TCCGGAAAGT TGTCAAAAAT ACATCTGGGA AAACTAGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031802411.1
CDS	1..4071
Translation	MSTGDSFETRFEKIDNLLRDPKSEVNSDCLLDGLDALVYDLDFPALRKNKNIDNFLSKYKDTINKIRDLRMKAEDYEVVKVIGRGAFGEVQLVRHKSTRKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLYYAFQDDRYLYMVMEYMPGGDLVNLMSNYDVPEKWARFYTAEVVLALDAIHSMGFIHRDVKPDNMLLDKSGHLKLADFGTCMKMNKEGMVRCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLYEMLVGDTPFYADSLVGTYSKIMNHKNSLTFPDDNDISKEAKNLICAFLTDREVRLGRNGVEEIKRHLFFKNDQWAWETLRDTVAPVVPDLSSDIDTSNFDDLEEDKGEEETFPIPKAFVGNQLPFVGFTYYSNRRYLSVSSANPNDNRTTSNIDKSLQESLQNTIYKLEEQLHNEIQLKDEMEQKCRTSNIKLDKIMKELDEEGNQRKNLESTVSQIEKEKMLLQHRVNEYQRKIEQENEKRRNVENEVSTLKDQLDDLKKVSQNSQLTNEKMAQLQKQLKEANDLLRTESDTAARLRKSHTELSKSVSQLESLNRELQERNRILETTKSQVDKDYYQLQAALEAERRDRSHGSEMIGDLQARITSLQEEVKHLKQNLERVEGERKEAQDMLNHSEKEKNNLEIDLNYKLKSLQQRLEQEVNEHKVTKARLTDKHQSIEEAKSVAMCEMEKKLKEERAAREKAENRVVHIEKQCSMLDVDLKQSQQKLEHLAEHKERMEDEVKNLTLQLEQESNKRLLIQNELKTQAFEKDNLKGLEKQMKQEINTLLEAKRLLEFELAQLTRQYCGNEGQMRELQDQLEAEQYFSTLYKTQVKELKEDIEEKNRENLKKIQELQNEKETLAAQLDLVETKAESEQLARRLLEEQYFELTQECKKAASRNKQEITDKDHTVNRLEEINSTLTKDIELLRKENEEFTEKMRKEQEDYKLKKEEEINNLKASFEKNINTERTLKTQAVNKLAEIMNRKDFKIDRKKANTQDLRKKEKENRKLQLELNQEREKFNQMVVKHQKELNEMQAQLVEECTHRNELQMQLASKESDIEQLRAKLLDLSDSTSVTSFPSADETDGNLPESRIEGWLSVPNRGNIKRYGWKKQYVVVSSKKILFYNDEQDKEQSNPSMVLDIDKLFHVRPVTQGDVYRAETEEIPKIFQILYANEGECRKDLEMEPVQQAEKTNFQNHKGHEFIPTLYHFPANCEACAKPLWHVFKPPPALECRRCHVKCHRDHLDKKEDLISPCKVSYDVTSARDMLLLACSQDEQKKWVTHLVKKIPKNPPSSFVRASPRTLSTRSSANQSFRKVVKNTSGKTS

Target ORF information:

RefSeq Version	XM_031946551.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii Rho associated coiled-coil containing protein kinase 1 (ROCK1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031946551.1

ORF Insert Sequence:

ATGTCGACTG GGGACAGTTT TGAGACTCGG TTTGAGAAAA TCGACAACCT GCTGCGGGAC 
CCGAAGTCGG AAGTGAATTC GGATTGTTTG CTGGATGGAT TGGATGCTTT GGTATATGAT 
TTGGATTTTC CTGCACTAAG AAAAAACAAA AATATTGACA ACTTTTTAAG CAAATATAAA 
GATACAATAA ATAAAATTAG AGATTTGCGC ATGAAAGCTG AAGATTATGA AGTGGTGAAA 
GTGATTGGTA GAGGTGCATT TGGAGAAGTG CAATTGGTAA GGCATAAATC TACCAGGAAG 
GTATATGCTA TGAAGCTGCT GAGCAAATTT GAGATGATTA AGAGATCAGA TTCTGCTTTC 
TTCTGGGAAG AACGGGACAT TATGGCTTTT GCTAATAGCC CTTGGGTCGT TCAGCTATAT 
TATGCATTCC AAGATGATCG GTATCTCTAC ATGGTAATGG AATATATGCC TGGTGGGGAT 
CTTGTTAATT TAATGAGCAA CTATGATGTG CCTGAAAAAT GGGCCCGTTT TTATACTGCG 
GAAGTAGTTC TTGCTTTGGA TGCAATCCAT TCAATGGGCT TTATTCATAG AGATGTGAAG 
CCTGATAACA TGCTGCTGGA TAAATCTGGT CACTTGAAGC TAGCTGATTT TGGTACTTGT 
ATGAAGATGA ATAAAGAAGG TATGGTACGA TGTGATACAG CAGTTGGAAC ACCTGACTAT 
ATTTCTCCTG AAGTATTAAA ATCCCAAGGT GGTGATGGCT ATTATGGGCG AGAATGTGAC 
TGGTGGTCTG TTGGTGTGTT TTTATATGAA ATGCTTGTAG GTGATACACC ATTTTATGCA 
GATTCACTGG TTGGAACATA TAGCAAAATT ATGAACCATA AGAATTCTCT TACCTTTCCT 
GATGATAATG ATATATCAAA GGAAGCAAAA AATCTTATTT GTGCCTTCCT TACAGACAGG 
GAAGTGAGAT TAGGACGAAA TGGTGTGGAA GAAATCAAAC GACATCTCTT CTTCAAAAAT 
GATCAATGGG CATGGGAAAC TCTTCGAGAT ACTGTTGCAC CTGTTGTACC TGATCTCAGT 
AGTGACATTG ATACTAGTAA TTTTGATGAC TTAGAAGAAG ACAAAGGAGA AGAAGAAACA 
TTTCCCATTC CAAAAGCTTT TGTTGGCAAT CAACTACCTT TTGTAGGATT TACATATTAT 
AGCAATCGTA GATATTTATC TGTATCTTCA GCCAATCCTA ATGATAATAG AACCACCTCC 
AATATAGATA AAAGCTTGCA GGAAAGTTTG CAAAATACAA TCTATAAGCT GGAAGAACAA 
TTGCATAATG AAATACAGCT AAAAGATGAA ATGGAGCAGA AGTGCAGAAC TTCAAACATA 
AAACTAGACA AGATAATGAA AGAACTGGAT GAAGAGGGAA ATCAAAGAAA AAATTTAGAA 
TCCACAGTGT CTCAGATTGA GAAAGAGAAA ATGTTGCTGC AGCACAGAGT TAATGAGTAC 
CAAAGAAAAA TAGAACAGGA GAATGAGAAG AGAAGGAATG TTGAAAATGA AGTTTCTACA 
TTAAAAGACC AATTGGATGA CCTGAAGAAA GTTAGTCAGA ATTCTCAGCT TACAAATGAG 
AAAATGGCAC AATTACAGAA GCAACTAAAA GAAGCGAATG ACTTACTGAG GACAGAATCA 
GACACAGCAG CAAGATTAAG AAAGAGCCAC ACAGAATTAA GCAAATCAGT AAGTCAGTTA 
GAGTCCCTAA ACAGAGAATT GCAAGAGCGA AACAGAATTT TAGAGACAAC CAAATCACAA 
GTGGACAAGG ATTATTACCA GCTTCAGGCT GCATTAGAAG CTGAAAGAAG AGACCGAAGT 
CATGGATCGG AGATGATAGG AGATCTTCAA GCTCGAATTA CATCTTTACA AGAAGAAGTG 
AAACACCTTA AACAAAATCT TGAAAGAGTG GAAGGGGAAA GAAAAGAAGC TCAAGACATG 
CTTAATCACT CAGAAAAGGA GAAGAATAAT TTGGAAATAG ATTTAAACTA CAAGCTAAAA 
TCATTACAAC AAAGATTAGA ACAGGAAGTA AATGAGCATA AAGTGACTAA AGCCCGTTTG 
ACTGACAAAC ATCAATCTAT TGAAGAAGCA AAGTCAGTTG CAATGTGTGA AATGGAAAAG 
AAACTGAAGG AAGAGAGAGC AGCCCGAGAG AAAGCTGAAA ATAGAGTTGT TCATATTGAA 
AAGCAGTGTT CTATGCTAGA TGTTGACTTG AAACAGTCTC AACAGAAACT GGAACATTTA 
GCAGAACATA AAGAAAGGAT GGAAGATGAA GTTAAGAATC TAACACTGCA GTTGGAGCAA 
GAATCAAATA AACGGTTGCT GATACAGAAT GAATTAAAAA CTCAAGCTTT TGAGAAGGAT 
AATTTGAAAG GGTTAGAAAA GCAAATGAAA CAAGAAATCA ATACATTATT GGAAGCAAAG 
AGATTACTGG AATTTGAACT AGCCCAGCTT ACAAGACAAT ATTGTGGAAA CGAAGGACAG 
ATGCGGGAAC TACAGGATCA GCTTGAAGCT GAACAATATT TTTCAACACT TTATAAAACT 
CAGGTAAAGG AACTTAAAGA GGACATTGAA GAAAAAAATC GAGAAAATCT AAAGAAAATA 
CAAGAGCTTC AAAATGAAAA AGAAACACTT GCTGCACAGT TAGACCTGGT AGAAACAAAA 
GCTGAATCAG AGCAGTTGGC TCGACGTCTA CTGGAAGAAC AATATTTTGA ATTGACTCAA 
GAGTGTAAAA AAGCAGCTTC ACGAAATAAA CAAGAAATTA CAGATAAAGA TCACACTGTC 
AATCGGCTGG AAGAAATAAA CAGTACATTA ACCAAAGATA TTGAATTATT AAGAAAGGAA 
AATGAAGAGT TTACTGAAAA AATGAGGAAG GAACAAGAAG ATTATAAATT GAAGAAAGAA 
GAAGAGATCA ATAATCTTAA AGCTTCTTTT GAAAAGAATA TTAATACTGA AAGAACTCTA 
AAAACGCAGG CTGTTAACAA ATTGGCTGAA ATAATGAATC GTAAAGATTT TAAAATTGAT 
AGAAAGAAGG CTAATACACA GGATTTAAGA AAGAAGGAAA AGGAAAACAG GAAGTTACAA 
CTGGAACTCA ACCAAGAAAG AGAGAAATTC AATCAAATGG TAGTGAAACA CCAGAAAGAA 
CTCAATGAAA TGCAAGCGCA ATTGGTTGAA GAGTGCACAC ACAGGAATGA ACTTCAGATG 
CAGCTGGCTA GCAAAGAGAG TGACATAGAG CAGTTGCGTG CAAAACTTTT GGACCTTTCA 
GATTCTACCA GTGTTACTAG TTTTCCTAGT GCCGATGAAA CTGATGGAAA CCTTCCAGAA 
TCAAGAATTG AAGGTTGGCT ATCGGTTCCA AACAGAGGAA ATATAAAACG ATATGGCTGG 
AAGAAACAGT ATGTCGTAGT AAGCAGCAAA AAAATTTTGT TCTACAATGA TGAACAGGAT 
AAGGAACAGT CAAATCCATC TATGGTACTA GACATAGACA AACTATTCCA TGTTCGACCT 
GTAACCCAAG GTGATGTGTA CAGAGCAGAA ACCGAAGAAA TCCCTAAAAT ATTCCAGATA 
TTGTATGCAA ATGAAGGTGA ATGTAGGAAA GATTTGGAAA TGGAGCCAGT ACAACAAGCT 
GAGAAGACCA ATTTTCAGAA TCACAAAGGC CATGAGTTTA TTCCTACACT CTATCACTTT 
CCAGCCAACT GTGAGGCTTG TGCCAAACCT CTCTGGCATG TTTTTAAGCC ACCCCCTGCC 
CTCGAATGTC GAAGATGCCA TGTCAAGTGC CATAGAGATC ACTTAGACAA AAAAGAAGAC 
TTAATTTCTC CATGCAAAGT GAGTTATGAT GTAACATCAG CAAGAGATAT GCTGCTGTTA 
GCATGTTCTC AGGATGAACA GAAGAAATGG GTAACCCATT TAGTAAAGAA GATCCCTAAG 
AATCCACCAT CCAGTTTTGT TCGTGCCTCC CCTAGAACAC TTTCTACTAG ATCTAGTGCA 
AATCAGTCTT TCCGGAAAGT TGTCAAAAAT ACATCTGGGA AAACTAGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROCK1 ( XM_023496213.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_023351981.1 Sarcophilus harrisii Rho associated coiled-coil containing protein kinase 1 (ROCK1), mRNA.

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CloneID	OSa158628
Clone ID Related Accession (Same CDS sequence)	XM_031946551.1 , XM_023496213.1
Accession Version	XM_023496213.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4071bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515513600000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	rho-associated protein kinase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003823314.1) and transcript sequences (GEDN01115839.1 and GEDN01115840.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 1130 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGTCGACTG GGGACAGTTT TGAGACTCGG TTTGAGAAAA TCGACAACCT GCTGCGGGAC 
CCGAAGTCGG AAGTGAATTC GGATTGTTTG CTGGATGGAT TGGATGCTTT GGTATATGAT 
TTGGATTTTC CTGCACTAAG AAAAAACAAA AATATTGACA ACTTTTTAAG CAAATATAAA 
GATACAATAA ATAAAATTAG AGATTTGCGC ATGAAAGCTG AAGATTATGA AGTGGTGAAA 
GTGATTGGTA GAGGTGCATT TGGAGAAGTG CAATTGGTAA GGCATAAATC TACCAGGAAG 
GTATATGCTA TGAAGCTGCT GAGCAAATTT GAGATGATTA AGAGATCAGA TTCTGCTTTC 
TTCTGGGAAG AACGGGACAT TATGGCTTTT GCTAATAGCC CTTGGGTCGT TCAGCTATAT 
TATGCATTCC AAGATGATCG GTATCTCTAC ATGGTAATGG AATATATGCC TGGTGGGGAT 
CTTGTTAATT TAATGAGCAA CTATGATGTG CCTGAAAAAT GGGCCCGTTT TTATACTGCG 
GAAGTAGTTC TTGCTTTGGA TGCAATCCAT TCAATGGGCT TTATTCATAG AGATGTGAAG 
CCTGATAACA TGCTGCTGGA TAAATCTGGT CACTTGAAGC TAGCTGATTT TGGTACTTGT 
ATGAAGATGA ATAAAGAAGG TATGGTACGA TGTGATACAG CAGTTGGAAC ACCTGACTAT 
ATTTCTCCTG AAGTATTAAA ATCCCAAGGT GGTGATGGCT ATTATGGGCG AGAATGTGAC 
TGGTGGTCTG TTGGTGTGTT TTTATATGAA ATGCTTGTAG GTGATACACC ATTTTATGCA 
GATTCACTGG TTGGAACATA TAGCAAAATT ATGAACCATA AGAATTCTCT TACCTTTCCT 
GATGATAATG ATATATCAAA GGAAGCAAAA AATCTTATTT GTGCCTTCCT TACAGACAGG 
GAAGTGAGAT TAGGACGAAA TGGTGTGGAA GAAATCAAAC GACATCTCTT CTTCAAAAAT 
GATCAATGGG CATGGGAAAC TCTTCGAGAT ACTGTTGCAC CTGTTGTACC TGATCTCAGT 
AGTGACATTG ATACTAGTAA TTTTGATGAC TTAGAAGAAG ACAAAGGAGA AGAAGAAACA 
TTTCCCATTC CAAAAGCTTT TGTTGGCAAT CAACTACCTT TTGTAGGATT TACATATTAT 
AGCAATCGTA GATATTTATC TGTATCTTCA GCCAATCCTA ATGATAATAG AACCACCTCC 
AATATAGATA AAAGCTTGCA GGAAAGTTTG CAAAATACAA TCTATAAGCT GGAAGAACAA 
TTGCATAATG AAATACAGCT AAAAGATGAA ATGGAGCAGA AGTGCAGAAC TTCAAACATA 
AAACTAGACA AGATAATGAA AGAACTGGAT GAAGAGGGAA ATCAAAGAAA AAATTTAGAA 
TCCACAGTGT CTCAGATTGA GAAAGAGAAA ATGTTGCTGC AGCACAGAGT TAATGAGTAC 
CAAAGAAAAA TAGAACAGGA GAATGAGAAG AGAAGGAATG TTGAAAATGA AGTTTCTACA 
TTAAAAGACC AATTGGATGA CCTGAAGAAA GTTAGTCAGA ATTCTCAGCT TACAAATGAG 
AAAATGGCAC AATTACAGAA GCAACTAAAA GAAGCGAATG ACTTACTGAG GACAGAATCA 
GACACAGCAG CAAGATTAAG AAAGAGCCAC ACAGAATTAA GCAAATCAGT AAGTCAGTTA 
GAGTCCCTAA ACAGAGAATT GCAAGAGCGA AACAGAATTT TAGAGACAAC CAAATCACAA 
GTGGACAAGG ATTATTACCA GCTTCAGGCT GCATTAGAAG CTGAAAGAAG AGACCGAAGT 
CATGGATCGG AGATGATAGG AGATCTTCAA GCTCGAATTA CATCTTTACA AGAAGAAGTG 
AAACACCTTA AACAAAATCT TGAAAGAGTG GAAGGGGAAA GAAAAGAAGC TCAAGACATG 
CTTAATCACT CAGAAAAGGA GAAGAATAAT TTGGAAATAG ATTTAAACTA CAAGCTAAAA 
TCATTACAAC AAAGATTAGA ACAGGAAGTA AATGAGCATA AAGTGACTAA AGCCCGTTTG 
ACTGACAAAC ATCAATCTAT TGAAGAAGCA AAGTCAGTTG CAATGTGTGA AATGGAAAAG 
AAACTGAAGG AAGAGAGAGC AGCCCGAGAG AAAGCTGAAA ATAGAGTTGT TCATATTGAA 
AAGCAGTGTT CTATGCTAGA TGTTGACTTG AAACAGTCTC AACAGAAACT GGAACATTTA 
GCAGAACATA AAGAAAGGAT GGAAGATGAA GTTAAGAATC TAACACTGCA GTTGGAGCAA 
GAATCAAATA AACGGTTGCT GATACAGAAT GAATTAAAAA CTCAAGCTTT TGAGAAGGAT 
AATTTGAAAG GGTTAGAAAA GCAAATGAAA CAAGAAATCA ATACATTATT GGAAGCAAAG 
AGATTACTGG AATTTGAACT AGCCCAGCTT ACAAGACAAT ATTGTGGAAA CGAAGGACAG 
ATGCGGGAAC TACAGGATCA GCTTGAAGCT GAACAATATT TTTCAACACT TTATAAAACT 
CAGGTAAAGG AACTTAAAGA GGACATTGAA GAAAAAAATC GAGAAAATCT AAAGAAAATA 
CAAGAGCTTC AAAATGAAAA AGAAACACTT GCTGCACAGT TAGACCTGGT AGAAACAAAA 
GCTGAATCAG AGCAGTTGGC TCGACGTCTA CTGGAAGAAC AATATTTTGA ATTGACTCAA 
GAGTGTAAAA AAGCAGCTTC ACGAAATAAA CAAGAAATTA CAGATAAAGA TCACACTGTC 
AATCGGCTGG AAGAAATAAA CAGTACATTA ACCAAAGATA TTGAATTATT AAGAAAGGAA 
AATGAAGAGT TTACTGAAAA AATGAGGAAG GAACAAGAAG ATTATAAATT GAAGAAAGAA 
GAAGAGATCA ATAATCTTAA AGCTTCTTTT GAAAAGAATA TTAATACTGA AAGAACTCTA 
AAAACGCAGG CTGTTAACAA ATTGGCTGAA ATAATGAATC GTAAAGATTT TAAAATTGAT 
AGAAAGAAGG CTAATACACA GGATTTAAGA AAGAAGGAAA AGGAAAACAG GAAGTTACAA 
CTGGAACTCA ACCAAGAAAG AGAGAAATTC AATCAAATGG TAGTGAAACA CCAGAAAGAA 
CTCAATGAAA TGCAAGCGCA ATTGGTTGAA GAGTGCACAC ACAGGAATGA ACTTCAGATG 
CAGCTGGCTA GCAAAGAGAG TGACATAGAG CAGTTGCGTG CAAAACTTTT GGACCTTTCA 
GATTCTACCA GTGTTACTAG TTTTCCTAGT GCCGATGAAA CTGATGGAAA CCTTCCAGAA 
TCAAGAATTG AAGGTTGGCT ATCGGTTCCA AACAGAGGAA ATATAAAACG ATATGGCTGG 
AAGAAACAGT ATGTCGTAGT AAGCAGCAAA AAAATTTTGT TCTACAATGA TGAACAGGAT 
AAGGAACAGT CAAATCCATC TATGGTACTA GACATAGACA AACTATTCCA TGTTCGACCT 
GTAACCCAAG GTGATGTGTA CAGAGCAGAA ACCGAAGAAA TCCCTAAAAT ATTCCAGATA 
TTGTATGCAA ATGAAGGTGA ATGTAGGAAA GATTTGGAAA TGGAGCCAGT ACAACAAGCT 
GAGAAGACCA ATTTTCAGAA TCACAAAGGC CATGAGTTTA TTCCTACACT CTATCACTTT 
CCAGCCAACT GTGAGGCTTG TGCCAAACCT CTCTGGCATG TTTTTAAGCC ACCCCCTGCC 
CTCGAATGTC GAAGATGCCA TGTCAAGTGC CATAGAGATC ACTTAGACAA AAAAGAAGAC 
TTAATTTCTC CATGCAAAGT GAGTTATGAT GTAACATCAG CAAGAGATAT GCTGCTGTTA 
GCATGTTCTC AGGATGAACA GAAGAAATGG GTAACCCATT TAGTAAAGAA GATCCCTAAG 
AATCCACCAT CCAGTTTTGT TCGTGCCTCC CCTAGAACAC TTTCTACTAG ATCTAGTGCA 
AATCAGTCTT TCCGGAAAGT TGTCAAAAAT ACATCTGGGA AAACTAGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023351981.1
CDS	722..4792
Translation	MSTGDSFETRFEKIDNLLRDPKSEVNSDCLLDGLDALVYDLDFPALRKNKNIDNFLSKYKDTINKIRDLRMKAEDYEVVKVIGRGAFGEVQLVRHKSTRKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLYYAFQDDRYLYMVMEYMPGGDLVNLMSNYDVPEKWARFYTAEVVLALDAIHSMGFIHRDVKPDNMLLDKSGHLKLADFGTCMKMNKEGMVRCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLYEMLVGDTPFYADSLVGTYSKIMNHKNSLTFPDDNDISKEAKNLICAFLTDREVRLGRNGVEEIKRHLFFKNDQWAWETLRDTVAPVVPDLSSDIDTSNFDDLEEDKGEEETFPIPKAFVGNQLPFVGFTYYSNRRYLSVSSANPNDNRTTSNIDKSLQESLQNTIYKLEEQLHNEIQLKDEMEQKCRTSNIKLDKIMKELDEEGNQRKNLESTVSQIEKEKMLLQHRVNEYQRKIEQENEKRRNVENEVSTLKDQLDDLKKVSQNSQLTNEKMAQLQKQLKEANDLLRTESDTAARLRKSHTELSKSVSQLESLNRELQERNRILETTKSQVDKDYYQLQAALEAERRDRSHGSEMIGDLQARITSLQEEVKHLKQNLERVEGERKEAQDMLNHSEKEKNNLEIDLNYKLKSLQQRLEQEVNEHKVTKARLTDKHQSIEEAKSVAMCEMEKKLKEERAAREKAENRVVHIEKQCSMLDVDLKQSQQKLEHLAEHKERMEDEVKNLTLQLEQESNKRLLIQNELKTQAFEKDNLKGLEKQMKQEINTLLEAKRLLEFELAQLTRQYCGNEGQMRELQDQLEAEQYFSTLYKTQVKELKEDIEEKNRENLKKIQELQNEKETLAAQLDLVETKAESEQLARRLLEEQYFELTQECKKAASRNKQEITDKDHTVNRLEEINSTLTKDIELLRKENEEFTEKMRKEQEDYKLKKEEEINNLKASFEKNINTERTLKTQAVNKLAEIMNRKDFKIDRKKANTQDLRKKEKENRKLQLELNQEREKFNQMVVKHQKELNEMQAQLVEECTHRNELQMQLASKESDIEQLRAKLLDLSDSTSVTSFPSADETDGNLPESRIEGWLSVPNRGNIKRYGWKKQYVVVSSKKILFYNDEQDKEQSNPSMVLDIDKLFHVRPVTQGDVYRAETEEIPKIFQILYANEGECRKDLEMEPVQQAEKTNFQNHKGHEFIPTLYHFPANCEACAKPLWHVFKPPPALECRRCHVKCHRDHLDKKEDLISPCKVSYDVTSARDMLLLACSQDEQKKWVTHLVKKIPKNPPSSFVRASPRTLSTRSSANQSFRKVVKNTSGKTS

Target ORF information:

RefSeq Version	XM_023496213.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii Rho associated coiled-coil containing protein kinase 1 (ROCK1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023496213.1

ORF Insert Sequence:

ATGTCGACTG GGGACAGTTT TGAGACTCGG TTTGAGAAAA TCGACAACCT GCTGCGGGAC 
CCGAAGTCGG AAGTGAATTC GGATTGTTTG CTGGATGGAT TGGATGCTTT GGTATATGAT 
TTGGATTTTC CTGCACTAAG AAAAAACAAA AATATTGACA ACTTTTTAAG CAAATATAAA 
GATACAATAA ATAAAATTAG AGATTTGCGC ATGAAAGCTG AAGATTATGA AGTGGTGAAA 
GTGATTGGTA GAGGTGCATT TGGAGAAGTG CAATTGGTAA GGCATAAATC TACCAGGAAG 
GTATATGCTA TGAAGCTGCT GAGCAAATTT GAGATGATTA AGAGATCAGA TTCTGCTTTC 
TTCTGGGAAG AACGGGACAT TATGGCTTTT GCTAATAGCC CTTGGGTCGT TCAGCTATAT 
TATGCATTCC AAGATGATCG GTATCTCTAC ATGGTAATGG AATATATGCC TGGTGGGGAT 
CTTGTTAATT TAATGAGCAA CTATGATGTG CCTGAAAAAT GGGCCCGTTT TTATACTGCG 
GAAGTAGTTC TTGCTTTGGA TGCAATCCAT TCAATGGGCT TTATTCATAG AGATGTGAAG 
CCTGATAACA TGCTGCTGGA TAAATCTGGT CACTTGAAGC TAGCTGATTT TGGTACTTGT 
ATGAAGATGA ATAAAGAAGG TATGGTACGA TGTGATACAG CAGTTGGAAC ACCTGACTAT 
ATTTCTCCTG AAGTATTAAA ATCCCAAGGT GGTGATGGCT ATTATGGGCG AGAATGTGAC 
TGGTGGTCTG TTGGTGTGTT TTTATATGAA ATGCTTGTAG GTGATACACC ATTTTATGCA 
GATTCACTGG TTGGAACATA TAGCAAAATT ATGAACCATA AGAATTCTCT TACCTTTCCT 
GATGATAATG ATATATCAAA GGAAGCAAAA AATCTTATTT GTGCCTTCCT TACAGACAGG 
GAAGTGAGAT TAGGACGAAA TGGTGTGGAA GAAATCAAAC GACATCTCTT CTTCAAAAAT 
GATCAATGGG CATGGGAAAC TCTTCGAGAT ACTGTTGCAC CTGTTGTACC TGATCTCAGT 
AGTGACATTG ATACTAGTAA TTTTGATGAC TTAGAAGAAG ACAAAGGAGA AGAAGAAACA 
TTTCCCATTC CAAAAGCTTT TGTTGGCAAT CAACTACCTT TTGTAGGATT TACATATTAT 
AGCAATCGTA GATATTTATC TGTATCTTCA GCCAATCCTA ATGATAATAG AACCACCTCC 
AATATAGATA AAAGCTTGCA GGAAAGTTTG CAAAATACAA TCTATAAGCT GGAAGAACAA 
TTGCATAATG AAATACAGCT AAAAGATGAA ATGGAGCAGA AGTGCAGAAC TTCAAACATA 
AAACTAGACA AGATAATGAA AGAACTGGAT GAAGAGGGAA ATCAAAGAAA AAATTTAGAA 
TCCACAGTGT CTCAGATTGA GAAAGAGAAA ATGTTGCTGC AGCACAGAGT TAATGAGTAC 
CAAAGAAAAA TAGAACAGGA GAATGAGAAG AGAAGGAATG TTGAAAATGA AGTTTCTACA 
TTAAAAGACC AATTGGATGA CCTGAAGAAA GTTAGTCAGA ATTCTCAGCT TACAAATGAG 
AAAATGGCAC AATTACAGAA GCAACTAAAA GAAGCGAATG ACTTACTGAG GACAGAATCA 
GACACAGCAG CAAGATTAAG AAAGAGCCAC ACAGAATTAA GCAAATCAGT AAGTCAGTTA 
GAGTCCCTAA ACAGAGAATT GCAAGAGCGA AACAGAATTT TAGAGACAAC CAAATCACAA 
GTGGACAAGG ATTATTACCA GCTTCAGGCT GCATTAGAAG CTGAAAGAAG AGACCGAAGT 
CATGGATCGG AGATGATAGG AGATCTTCAA GCTCGAATTA CATCTTTACA AGAAGAAGTG 
AAACACCTTA AACAAAATCT TGAAAGAGTG GAAGGGGAAA GAAAAGAAGC TCAAGACATG 
CTTAATCACT CAGAAAAGGA GAAGAATAAT TTGGAAATAG ATTTAAACTA CAAGCTAAAA 
TCATTACAAC AAAGATTAGA ACAGGAAGTA AATGAGCATA AAGTGACTAA AGCCCGTTTG 
ACTGACAAAC ATCAATCTAT TGAAGAAGCA AAGTCAGTTG CAATGTGTGA AATGGAAAAG 
AAACTGAAGG AAGAGAGAGC AGCCCGAGAG AAAGCTGAAA ATAGAGTTGT TCATATTGAA 
AAGCAGTGTT CTATGCTAGA TGTTGACTTG AAACAGTCTC AACAGAAACT GGAACATTTA 
GCAGAACATA AAGAAAGGAT GGAAGATGAA GTTAAGAATC TAACACTGCA GTTGGAGCAA 
GAATCAAATA AACGGTTGCT GATACAGAAT GAATTAAAAA CTCAAGCTTT TGAGAAGGAT 
AATTTGAAAG GGTTAGAAAA GCAAATGAAA CAAGAAATCA ATACATTATT GGAAGCAAAG 
AGATTACTGG AATTTGAACT AGCCCAGCTT ACAAGACAAT ATTGTGGAAA CGAAGGACAG 
ATGCGGGAAC TACAGGATCA GCTTGAAGCT GAACAATATT TTTCAACACT TTATAAAACT 
CAGGTAAAGG AACTTAAAGA GGACATTGAA GAAAAAAATC GAGAAAATCT AAAGAAAATA 
CAAGAGCTTC AAAATGAAAA AGAAACACTT GCTGCACAGT TAGACCTGGT AGAAACAAAA 
GCTGAATCAG AGCAGTTGGC TCGACGTCTA CTGGAAGAAC AATATTTTGA ATTGACTCAA 
GAGTGTAAAA AAGCAGCTTC ACGAAATAAA CAAGAAATTA CAGATAAAGA TCACACTGTC 
AATCGGCTGG AAGAAATAAA CAGTACATTA ACCAAAGATA TTGAATTATT AAGAAAGGAA 
AATGAAGAGT TTACTGAAAA AATGAGGAAG GAACAAGAAG ATTATAAATT GAAGAAAGAA 
GAAGAGATCA ATAATCTTAA AGCTTCTTTT GAAAAGAATA TTAATACTGA AAGAACTCTA 
AAAACGCAGG CTGTTAACAA ATTGGCTGAA ATAATGAATC GTAAAGATTT TAAAATTGAT 
AGAAAGAAGG CTAATACACA GGATTTAAGA AAGAAGGAAA AGGAAAACAG GAAGTTACAA 
CTGGAACTCA ACCAAGAAAG AGAGAAATTC AATCAAATGG TAGTGAAACA CCAGAAAGAA 
CTCAATGAAA TGCAAGCGCA ATTGGTTGAA GAGTGCACAC ACAGGAATGA ACTTCAGATG 
CAGCTGGCTA GCAAAGAGAG TGACATAGAG CAGTTGCGTG CAAAACTTTT GGACCTTTCA 
GATTCTACCA GTGTTACTAG TTTTCCTAGT GCCGATGAAA CTGATGGAAA CCTTCCAGAA 
TCAAGAATTG AAGGTTGGCT ATCGGTTCCA AACAGAGGAA ATATAAAACG ATATGGCTGG 
AAGAAACAGT ATGTCGTAGT AAGCAGCAAA AAAATTTTGT TCTACAATGA TGAACAGGAT 
AAGGAACAGT CAAATCCATC TATGGTACTA GACATAGACA AACTATTCCA TGTTCGACCT 
GTAACCCAAG GTGATGTGTA CAGAGCAGAA ACCGAAGAAA TCCCTAAAAT ATTCCAGATA 
TTGTATGCAA ATGAAGGTGA ATGTAGGAAA GATTTGGAAA TGGAGCCAGT ACAACAAGCT 
GAGAAGACCA ATTTTCAGAA TCACAAAGGC CATGAGTTTA TTCCTACACT CTATCACTTT 
CCAGCCAACT GTGAGGCTTG TGCCAAACCT CTCTGGCATG TTTTTAAGCC ACCCCCTGCC 
CTCGAATGTC GAAGATGCCA TGTCAAGTGC CATAGAGATC ACTTAGACAA AAAAGAAGAC 
TTAATTTCTC CATGCAAAGT GAGTTATGAT GTAACATCAG CAAGAGATAT GCTGCTGTTA 
GCATGTTCTC AGGATGAACA GAAGAAATGG GTAACCCATT TAGTAAAGAA GATCCCTAAG 
AATCCACCAT CCAGTTTTGT TCGTGCCTCC CCTAGAACAC TTTCTACTAG ATCTAGTGCA 
AATCAGTCTT TCCGGAAAGT TGTCAAAAAT ACATCTGGGA AAACTAGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROCK1 ( XM_031946552.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031802412.1 Sarcophilus harrisii Rho associated coiled-coil containing protein kinase 1 (ROCK1), transcript variant X2, mRNA.

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CloneID	OSa221368
Clone ID Related Accession (Same CDS sequence)	XM_031946552.1
Accession Version	XM_031946552.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4068bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	rho-associated protein kinase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045426.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGACTG GGGACAGTTT TGAGACTCGG TTTGAGAAAA TCGACAACCT GCTGCGGGAC 
CCGAAGTCGG AAGTGAATTC GGATTGTTTG CTGGATGGAT TGGATGCTTT GGTATATGAT 
TTGGATTTTC CTGCACTAAG AAAAAACAAA AATATTGACA ACTTTTTAAG CAAATATAAA 
GATACAATAA ATAAAATTAG AGATTTGCGC ATGAAAGCTG AAGATTATGA AGTGGTGAAA 
GTGATTGGTA GAGGTGCATT TGGAGAAGTG CAATTGGTAA GGCATAAATC TACCAGGAAG 
GTATATGCTA TGAAGCTGCT GAGCAAATTT GAGATGATTA AGAGATCAGA TTCTGCTTTC 
TTCTGGGAAG AACGGGACAT TATGGCTTTT GCTAATAGCC CTTGGGTCGT TCAGCTATAT 
TATGCATTCC AAGATGATCG GTATCTCTAC ATGGTAATGG AATATATGCC TGGTGGGGAT 
CTTGTTAATT TAATGAGCAA CTATGATGTG CCTGAAAAAT GGGCCCGTTT TTATACTGCG 
GAAGTAGTTC TTGCTTTGGA TGCAATCCAT TCAATGGGCT TTATTCATAG AGATGTGAAG 
CCTGATAACA TGCTGCTGGA TAAATCTGGT CACTTGAAGC TAGCTGATTT TGGTACTTGT 
ATGAAGATGA ATAAAGAAGG TATGGTACGA TGTGATACAG CAGTTGGAAC ACCTGACTAT 
ATTTCTCCTG AAGTATTAAA ATCCCAAGGT GGTGATGGCT ATTATGGGCG AGAATGTGAC 
TGGTGGTCTG TTGGTGTGTT TTTATATGAA ATGCTTGTAG GTGATACACC ATTTTATGCA 
GATTCACTGG TTGGAACATA TAGCAAAATT ATGAACCATA AGAATTCTCT TACCTTTCCT 
GATGATAATG ATATATCAAA GGAAGCAAAA AATCTTATTT GTGCCTTCCT TACAGACAGG 
GAAGTGAGAT TAGGACGAAA TGGTGTGGAA GAAATCAAAC GACATCTCTT CTTCAAAAAT 
GATCAATGGG CATGGGAAAC TCTTCGAGAT ACTGTTGCAC CTGTTGTACC TGATCTCAGT 
AGTGACATTG ATACTAGTAA TTTTGATGAC TTAGAAGAAG ACAAAGGAGA AGAAGAAACA 
TTTCCCATTC CAAAAGCTTT TGTTGGCAAT CAACTACCTT TTGTAGGATT TACATATTAT 
AGCAATCGTA GATATTTATC TGTATCTTCA GCCAATCCTA ATGATAATAG AACCACCTCC 
AATATAGATA AAAGCTTGGA AAGTTTGCAA AATACAATCT ATAAGCTGGA AGAACAATTG 
CATAATGAAA TACAGCTAAA AGATGAAATG GAGCAGAAGT GCAGAACTTC AAACATAAAA 
CTAGACAAGA TAATGAAAGA ACTGGATGAA GAGGGAAATC AAAGAAAAAA TTTAGAATCC 
ACAGTGTCTC AGATTGAGAA AGAGAAAATG TTGCTGCAGC ACAGAGTTAA TGAGTACCAA 
AGAAAAATAG AACAGGAGAA TGAGAAGAGA AGGAATGTTG AAAATGAAGT TTCTACATTA 
AAAGACCAAT TGGATGACCT GAAGAAAGTT AGTCAGAATT CTCAGCTTAC AAATGAGAAA 
ATGGCACAAT TACAGAAGCA ACTAAAAGAA GCGAATGACT TACTGAGGAC AGAATCAGAC 
ACAGCAGCAA GATTAAGAAA GAGCCACACA GAATTAAGCA AATCAGTAAG TCAGTTAGAG 
TCCCTAAACA GAGAATTGCA AGAGCGAAAC AGAATTTTAG AGACAACCAA ATCACAAGTG 
GACAAGGATT ATTACCAGCT TCAGGCTGCA TTAGAAGCTG AAAGAAGAGA CCGAAGTCAT 
GGATCGGAGA TGATAGGAGA TCTTCAAGCT CGAATTACAT CTTTACAAGA AGAAGTGAAA 
CACCTTAAAC AAAATCTTGA AAGAGTGGAA GGGGAAAGAA AAGAAGCTCA AGACATGCTT 
AATCACTCAG AAAAGGAGAA GAATAATTTG GAAATAGATT TAAACTACAA GCTAAAATCA 
TTACAACAAA GATTAGAACA GGAAGTAAAT GAGCATAAAG TGACTAAAGC CCGTTTGACT 
GACAAACATC AATCTATTGA AGAAGCAAAG TCAGTTGCAA TGTGTGAAAT GGAAAAGAAA 
CTGAAGGAAG AGAGAGCAGC CCGAGAGAAA GCTGAAAATA GAGTTGTTCA TATTGAAAAG 
CAGTGTTCTA TGCTAGATGT TGACTTGAAA CAGTCTCAAC AGAAACTGGA ACATTTAGCA 
GAACATAAAG AAAGGATGGA AGATGAAGTT AAGAATCTAA CACTGCAGTT GGAGCAAGAA 
TCAAATAAAC GGTTGCTGAT ACAGAATGAA TTAAAAACTC AAGCTTTTGA GAAGGATAAT 
TTGAAAGGGT TAGAAAAGCA AATGAAACAA GAAATCAATA CATTATTGGA AGCAAAGAGA 
TTACTGGAAT TTGAACTAGC CCAGCTTACA AGACAATATT GTGGAAACGA AGGACAGATG 
CGGGAACTAC AGGATCAGCT TGAAGCTGAA CAATATTTTT CAACACTTTA TAAAACTCAG 
GTAAAGGAAC TTAAAGAGGA CATTGAAGAA AAAAATCGAG AAAATCTAAA GAAAATACAA 
GAGCTTCAAA ATGAAAAAGA AACACTTGCT GCACAGTTAG ACCTGGTAGA AACAAAAGCT 
GAATCAGAGC AGTTGGCTCG ACGTCTACTG GAAGAACAAT ATTTTGAATT GACTCAAGAG 
TGTAAAAAAG CAGCTTCACG AAATAAACAA GAAATTACAG ATAAAGATCA CACTGTCAAT 
CGGCTGGAAG AAATAAACAG TACATTAACC AAAGATATTG AATTATTAAG AAAGGAAAAT 
GAAGAGTTTA CTGAAAAAAT GAGGAAGGAA CAAGAAGATT ATAAATTGAA GAAAGAAGAA 
GAGATCAATA ATCTTAAAGC TTCTTTTGAA AAGAATATTA ATACTGAAAG AACTCTAAAA 
ACGCAGGCTG TTAACAAATT GGCTGAAATA ATGAATCGTA AAGATTTTAA AATTGATAGA 
AAGAAGGCTA ATACACAGGA TTTAAGAAAG AAGGAAAAGG AAAACAGGAA GTTACAACTG 
GAACTCAACC AAGAAAGAGA GAAATTCAAT CAAATGGTAG TGAAACACCA GAAAGAACTC 
AATGAAATGC AAGCGCAATT GGTTGAAGAG TGCACACACA GGAATGAACT TCAGATGCAG 
CTGGCTAGCA AAGAGAGTGA CATAGAGCAG TTGCGTGCAA AACTTTTGGA CCTTTCAGAT 
TCTACCAGTG TTACTAGTTT TCCTAGTGCC GATGAAACTG ATGGAAACCT TCCAGAATCA 
AGAATTGAAG GTTGGCTATC GGTTCCAAAC AGAGGAAATA TAAAACGATA TGGCTGGAAG 
AAACAGTATG TCGTAGTAAG CAGCAAAAAA ATTTTGTTCT ACAATGATGA ACAGGATAAG 
GAACAGTCAA ATCCATCTAT GGTACTAGAC ATAGACAAAC TATTCCATGT TCGACCTGTA 
ACCCAAGGTG ATGTGTACAG AGCAGAAACC GAAGAAATCC CTAAAATATT CCAGATATTG 
TATGCAAATG AAGGTGAATG TAGGAAAGAT TTGGAAATGG AGCCAGTACA ACAAGCTGAG 
AAGACCAATT TTCAGAATCA CAAAGGCCAT GAGTTTATTC CTACACTCTA TCACTTTCCA 
GCCAACTGTG AGGCTTGTGC CAAACCTCTC TGGCATGTTT TTAAGCCACC CCCTGCCCTC 
GAATGTCGAA GATGCCATGT CAAGTGCCAT AGAGATCACT TAGACAAAAA AGAAGACTTA 
ATTTCTCCAT GCAAAGTGAG TTATGATGTA ACATCAGCAA GAGATATGCT GCTGTTAGCA 
TGTTCTCAGG ATGAACAGAA GAAATGGGTA ACCCATTTAG TAAAGAAGAT CCCTAAGAAT 
CCACCATCCA GTTTTGTTCG TGCCTCCCCT AGAACACTTT CTACTAGATC TAGTGCAAAT 
CAGTCTTTCC GGAAAGTTGT CAAAAATACA TCTGGGAAAA CTAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031802412.1
CDS	229..4296
Translation	MSTGDSFETRFEKIDNLLRDPKSEVNSDCLLDGLDALVYDLDFPALRKNKNIDNFLSKYKDTINKIRDLRMKAEDYEVVKVIGRGAFGEVQLVRHKSTRKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLYYAFQDDRYLYMVMEYMPGGDLVNLMSNYDVPEKWARFYTAEVVLALDAIHSMGFIHRDVKPDNMLLDKSGHLKLADFGTCMKMNKEGMVRCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLYEMLVGDTPFYADSLVGTYSKIMNHKNSLTFPDDNDISKEAKNLICAFLTDREVRLGRNGVEEIKRHLFFKNDQWAWETLRDTVAPVVPDLSSDIDTSNFDDLEEDKGEEETFPIPKAFVGNQLPFVGFTYYSNRRYLSVSSANPNDNRTTSNIDKSLESLQNTIYKLEEQLHNEIQLKDEMEQKCRTSNIKLDKIMKELDEEGNQRKNLESTVSQIEKEKMLLQHRVNEYQRKIEQENEKRRNVENEVSTLKDQLDDLKKVSQNSQLTNEKMAQLQKQLKEANDLLRTESDTAARLRKSHTELSKSVSQLESLNRELQERNRILETTKSQVDKDYYQLQAALEAERRDRSHGSEMIGDLQARITSLQEEVKHLKQNLERVEGERKEAQDMLNHSEKEKNNLEIDLNYKLKSLQQRLEQEVNEHKVTKARLTDKHQSIEEAKSVAMCEMEKKLKEERAAREKAENRVVHIEKQCSMLDVDLKQSQQKLEHLAEHKERMEDEVKNLTLQLEQESNKRLLIQNELKTQAFEKDNLKGLEKQMKQEINTLLEAKRLLEFELAQLTRQYCGNEGQMRELQDQLEAEQYFSTLYKTQVKELKEDIEEKNRENLKKIQELQNEKETLAAQLDLVETKAESEQLARRLLEEQYFELTQECKKAASRNKQEITDKDHTVNRLEEINSTLTKDIELLRKENEEFTEKMRKEQEDYKLKKEEEINNLKASFEKNINTERTLKTQAVNKLAEIMNRKDFKIDRKKANTQDLRKKEKENRKLQLELNQEREKFNQMVVKHQKELNEMQAQLVEECTHRNELQMQLASKESDIEQLRAKLLDLSDSTSVTSFPSADETDGNLPESRIEGWLSVPNRGNIKRYGWKKQYVVVSSKKILFYNDEQDKEQSNPSMVLDIDKLFHVRPVTQGDVYRAETEEIPKIFQILYANEGECRKDLEMEPVQQAEKTNFQNHKGHEFIPTLYHFPANCEACAKPLWHVFKPPPALECRRCHVKCHRDHLDKKEDLISPCKVSYDVTSARDMLLLACSQDEQKKWVTHLVKKIPKNPPSSFVRASPRTLSTRSSANQSFRKVVKNTSGKTS

Target ORF information:

RefSeq Version	XM_031946552.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii Rho associated coiled-coil containing protein kinase 1 (ROCK1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031946552.1

ORF Insert Sequence:

ATGTCGACTG GGGACAGTTT TGAGACTCGG TTTGAGAAAA TCGACAACCT GCTGCGGGAC 
CCGAAGTCGG AAGTGAATTC GGATTGTTTG CTGGATGGAT TGGATGCTTT GGTATATGAT 
TTGGATTTTC CTGCACTAAG AAAAAACAAA AATATTGACA ACTTTTTAAG CAAATATAAA 
GATACAATAA ATAAAATTAG AGATTTGCGC ATGAAAGCTG AAGATTATGA AGTGGTGAAA 
GTGATTGGTA GAGGTGCATT TGGAGAAGTG CAATTGGTAA GGCATAAATC TACCAGGAAG 
GTATATGCTA TGAAGCTGCT GAGCAAATTT GAGATGATTA AGAGATCAGA TTCTGCTTTC 
TTCTGGGAAG AACGGGACAT TATGGCTTTT GCTAATAGCC CTTGGGTCGT TCAGCTATAT 
TATGCATTCC AAGATGATCG GTATCTCTAC ATGGTAATGG AATATATGCC TGGTGGGGAT 
CTTGTTAATT TAATGAGCAA CTATGATGTG CCTGAAAAAT GGGCCCGTTT TTATACTGCG 
GAAGTAGTTC TTGCTTTGGA TGCAATCCAT TCAATGGGCT TTATTCATAG AGATGTGAAG 
CCTGATAACA TGCTGCTGGA TAAATCTGGT CACTTGAAGC TAGCTGATTT TGGTACTTGT 
ATGAAGATGA ATAAAGAAGG TATGGTACGA TGTGATACAG CAGTTGGAAC ACCTGACTAT 
ATTTCTCCTG AAGTATTAAA ATCCCAAGGT GGTGATGGCT ATTATGGGCG AGAATGTGAC 
TGGTGGTCTG TTGGTGTGTT TTTATATGAA ATGCTTGTAG GTGATACACC ATTTTATGCA 
GATTCACTGG TTGGAACATA TAGCAAAATT ATGAACCATA AGAATTCTCT TACCTTTCCT 
GATGATAATG ATATATCAAA GGAAGCAAAA AATCTTATTT GTGCCTTCCT TACAGACAGG 
GAAGTGAGAT TAGGACGAAA TGGTGTGGAA GAAATCAAAC GACATCTCTT CTTCAAAAAT 
GATCAATGGG CATGGGAAAC TCTTCGAGAT ACTGTTGCAC CTGTTGTACC TGATCTCAGT 
AGTGACATTG ATACTAGTAA TTTTGATGAC TTAGAAGAAG ACAAAGGAGA AGAAGAAACA 
TTTCCCATTC CAAAAGCTTT TGTTGGCAAT CAACTACCTT TTGTAGGATT TACATATTAT 
AGCAATCGTA GATATTTATC TGTATCTTCA GCCAATCCTA ATGATAATAG AACCACCTCC 
AATATAGATA AAAGCTTGGA AAGTTTGCAA AATACAATCT ATAAGCTGGA AGAACAATTG 
CATAATGAAA TACAGCTAAA AGATGAAATG GAGCAGAAGT GCAGAACTTC AAACATAAAA 
CTAGACAAGA TAATGAAAGA ACTGGATGAA GAGGGAAATC AAAGAAAAAA TTTAGAATCC 
ACAGTGTCTC AGATTGAGAA AGAGAAAATG TTGCTGCAGC ACAGAGTTAA TGAGTACCAA 
AGAAAAATAG AACAGGAGAA TGAGAAGAGA AGGAATGTTG AAAATGAAGT TTCTACATTA 
AAAGACCAAT TGGATGACCT GAAGAAAGTT AGTCAGAATT CTCAGCTTAC AAATGAGAAA 
ATGGCACAAT TACAGAAGCA ACTAAAAGAA GCGAATGACT TACTGAGGAC AGAATCAGAC 
ACAGCAGCAA GATTAAGAAA GAGCCACACA GAATTAAGCA AATCAGTAAG TCAGTTAGAG 
TCCCTAAACA GAGAATTGCA AGAGCGAAAC AGAATTTTAG AGACAACCAA ATCACAAGTG 
GACAAGGATT ATTACCAGCT TCAGGCTGCA TTAGAAGCTG AAAGAAGAGA CCGAAGTCAT 
GGATCGGAGA TGATAGGAGA TCTTCAAGCT CGAATTACAT CTTTACAAGA AGAAGTGAAA 
CACCTTAAAC AAAATCTTGA AAGAGTGGAA GGGGAAAGAA AAGAAGCTCA AGACATGCTT 
AATCACTCAG AAAAGGAGAA GAATAATTTG GAAATAGATT TAAACTACAA GCTAAAATCA 
TTACAACAAA GATTAGAACA GGAAGTAAAT GAGCATAAAG TGACTAAAGC CCGTTTGACT 
GACAAACATC AATCTATTGA AGAAGCAAAG TCAGTTGCAA TGTGTGAAAT GGAAAAGAAA 
CTGAAGGAAG AGAGAGCAGC CCGAGAGAAA GCTGAAAATA GAGTTGTTCA TATTGAAAAG 
CAGTGTTCTA TGCTAGATGT TGACTTGAAA CAGTCTCAAC AGAAACTGGA ACATTTAGCA 
GAACATAAAG AAAGGATGGA AGATGAAGTT AAGAATCTAA CACTGCAGTT GGAGCAAGAA 
TCAAATAAAC GGTTGCTGAT ACAGAATGAA TTAAAAACTC AAGCTTTTGA GAAGGATAAT 
TTGAAAGGGT TAGAAAAGCA AATGAAACAA GAAATCAATA CATTATTGGA AGCAAAGAGA 
TTACTGGAAT TTGAACTAGC CCAGCTTACA AGACAATATT GTGGAAACGA AGGACAGATG 
CGGGAACTAC AGGATCAGCT TGAAGCTGAA CAATATTTTT CAACACTTTA TAAAACTCAG 
GTAAAGGAAC TTAAAGAGGA CATTGAAGAA AAAAATCGAG AAAATCTAAA GAAAATACAA 
GAGCTTCAAA ATGAAAAAGA AACACTTGCT GCACAGTTAG ACCTGGTAGA AACAAAAGCT 
GAATCAGAGC AGTTGGCTCG ACGTCTACTG GAAGAACAAT ATTTTGAATT GACTCAAGAG 
TGTAAAAAAG CAGCTTCACG AAATAAACAA GAAATTACAG ATAAAGATCA CACTGTCAAT 
CGGCTGGAAG AAATAAACAG TACATTAACC AAAGATATTG AATTATTAAG AAAGGAAAAT 
GAAGAGTTTA CTGAAAAAAT GAGGAAGGAA CAAGAAGATT ATAAATTGAA GAAAGAAGAA 
GAGATCAATA ATCTTAAAGC TTCTTTTGAA AAGAATATTA ATACTGAAAG AACTCTAAAA 
ACGCAGGCTG TTAACAAATT GGCTGAAATA ATGAATCGTA AAGATTTTAA AATTGATAGA 
AAGAAGGCTA ATACACAGGA TTTAAGAAAG AAGGAAAAGG AAAACAGGAA GTTACAACTG 
GAACTCAACC AAGAAAGAGA GAAATTCAAT CAAATGGTAG TGAAACACCA GAAAGAACTC 
AATGAAATGC AAGCGCAATT GGTTGAAGAG TGCACACACA GGAATGAACT TCAGATGCAG 
CTGGCTAGCA AAGAGAGTGA CATAGAGCAG TTGCGTGCAA AACTTTTGGA CCTTTCAGAT 
TCTACCAGTG TTACTAGTTT TCCTAGTGCC GATGAAACTG ATGGAAACCT TCCAGAATCA 
AGAATTGAAG GTTGGCTATC GGTTCCAAAC AGAGGAAATA TAAAACGATA TGGCTGGAAG 
AAACAGTATG TCGTAGTAAG CAGCAAAAAA ATTTTGTTCT ACAATGATGA ACAGGATAAG 
GAACAGTCAA ATCCATCTAT GGTACTAGAC ATAGACAAAC TATTCCATGT TCGACCTGTA 
ACCCAAGGTG ATGTGTACAG AGCAGAAACC GAAGAAATCC CTAAAATATT CCAGATATTG 
TATGCAAATG AAGGTGAATG TAGGAAAGAT TTGGAAATGG AGCCAGTACA ACAAGCTGAG 
AAGACCAATT TTCAGAATCA CAAAGGCCAT GAGTTTATTC CTACACTCTA TCACTTTCCA 
GCCAACTGTG AGGCTTGTGC CAAACCTCTC TGGCATGTTT TTAAGCCACC CCCTGCCCTC 
GAATGTCGAA GATGCCATGT CAAGTGCCAT AGAGATCACT TAGACAAAAA AGAAGACTTA 
ATTTCTCCAT GCAAAGTGAG TTATGATGTA ACATCAGCAA GAGATATGCT GCTGTTAGCA 
TGTTCTCAGG ATGAACAGAA GAAATGGGTA ACCCATTTAG TAAAGAAGAT CCCTAAGAAT 
CCACCATCCA GTTTTGTTCG TGCCTCCCCT AGAACACTTT CTACTAGATC TAGTGCAAAT 
CAGTCTTTCC GGAAAGTTGT CAAAAATACA TCTGGGAAAA CTAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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ROCK1 cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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