COL27A1 cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene
Clones
gRNAs

Summary

Gene Symbol	COL27A1
Entrez Gene ID	100915207
Full Name	collagen type XXVII alpha 1 chain
Gene Type	protein-coding
Organism	Sarcophilus harrisii(Tasmanian devil)

mRNA and Protein(s)

mRNA	Protein	Name
XM_023494251.1	XP_023350019.1	collagen alpha-1(XXVII) chain isoform X2
XM_031954623.1	XP_031810483.1	collagen alpha-1(XXVII) chain isoform X2
XM_003757352.1	XP_003757400.1	collagen alpha-1(XXVII) chain
XM_023494250.1	XP_023350018.1	collagen alpha-1(XXVII) chain isoform X1
XM_023494252.1	XP_023350020.1	collagen alpha-1(XXVII) chain isoform X3
XM_023494250.2	XP_023350018.1	collagen alpha-1(XXVII) chain isoform X1

Pathways from BioSystems
Gene Ontology

The following COL27A1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL27A1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSa118885	XM_003757352.1 Latest version!	Sarcophilus harrisii collagen, type XXVII, alpha 1 (COL27A1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSa150306	XM_023494250.2 Latest version!	Sarcophilus harrisii collagen type XXVII alpha 1 chain (COL27A1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSa150307	XM_023494251.1 Latest version!	Sarcophilus harrisii collagen type XXVII alpha 1 chain (COL27A1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSa150308	XM_023494252.1 Latest version!	Sarcophilus harrisii collagen type XXVII alpha 1 chain (COL27A1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSa226381	XM_031954623.1 Latest version!	Sarcophilus harrisii collagen type XXVII alpha 1 chain (COL27A1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
OSa150306	XM_023494250.1	Sarcophilus harrisii collagen type XXVII alpha 1 chain (COL27A1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

COL27A1 ( XM_003757352.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_003757400.1 Sarcophilus harrisii collagen, type XXVII, alpha 1 (COL27A1), mRNA.

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CloneID	OSa118885
Clone ID Related Accession (Same CDS sequence)	XM_003757352.1
Accession Version	XM_003757352.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5751bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1431532800000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	collagen alpha-1(XXVII) chain
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003816661.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sarcophilus harrisii Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 2% of CDS bases ##RefSeq-Attributes-END##

ATGATACTGA AGAAGGACCC ATGGTGTCAA TGGGCAGGTG GCAACACTTT GCTGCCACCA 
CCAATCACTT ATCAATGGTT ATCAGTGATC AGGGGATTCC TCCTAGCCTG GATTGCCTTC 
TGCTCTACCT GTGGCCTGGC CATCACCCAA GGAGATCCTG AAGATGTGGA TGTCCTGCAA 
CGGCTGGGCC TCGGGGGGAA GAAGTTGCCC GGCGGCCGGG CCCCAGGGCT GGCCCCCATC 
CCCCCAGGAG TCATCCCGTT CCAGTCGGGG TTCATCTTCA CCCAGCGGGC CCGGATTGAA 
GCCCTTACAG GCGCCATCAT TCCCACCACC TTCGGCACTG ACCTGACCCT GGTGCTGAGC 
CTGTGCTCCC ACCGAGTCAA CAACGCCTTC CTCTTTGCTG TCCGGAGCCG CAGGAAGAAG 
CTGCAGCTGG GGCTCCAGTT CATCCCTGGC AAGATCGTGC TGTTCCTGGG CCAGCGGCGC 
TCCCTGGCCT TCAGCTACGA CGTGCATGAC GGGCGATGGC ACCACTTGGC CATCAGTCTC 
CGAGGCCAGG GGGCCACCCT CCTCACCTCC TGCGGGAAGC ACCGCGTGCC CATGGATCTG 
CATTTTAAGA AAGAGGACTC TCTTGATCCC GAGGGGTCTT TCCTCTTTGG GAAGATGAAT 
CAGCACTCAG TCCAATTTGA GGGCGCCGTA TGCCAGTTCA ATATTTATCC TTCTGCCAAG 
GCAGCTCACA ATTATTGTAA ATATCTGAGG AAGCAGTGCA GACAGGCCGA CACCTACCGG 
CCACTGCTGC CCCCTCTTCT TCCCCTCCTT CCCCAGGACT CCGAGGCCCA TACCCATCTT 
GGCCTCCAGC CCAGCACCTC TCTGCAGAGC CTGAGGAATC TGACCACTGC GATGCCTTCG 
CTGGCACCCC TTAGAATGGC CAAGACCACC CCGGCCTTGC CCAGAGCCCC CGGCCAAGGT 
GCCAGGGCTA CATGGGTGCC CTCAGCACCG GCCGGGCTTG GGGCACCCCC CCACCCGCCT 
GTGAGGACTA GTGCCGCGGA TCCCCGGGCG TCCCGATCCC CCACAGTGGG TACTTTGGTC 
TGGACCCTAC CAGCAACTGC AAAGCCATTG CCTTTCTCTT CAGGCAAAGC TTCCTCACCT 
GGGCTCAGGC CTGCACTGAT CACCCCTCAG AGCAACTTGA CCAAATCTCC CGTTCGCGCC 
ATGAAGCCCA TGCCCCCATC CAAGGCCAGT GTGGTTAAAA CTCCCCTTGT TCCCAGTAAG 
CCAGTAACTC CTTCAGTCAC TGTGGTCCCA GCTAAGAGGC CCCATCCCCT GGTCAAGCCG 
GTGCTGACCT CCCCTGCTAA GAAGCCAGTG CCCTCTGGCT CTCAGCCTGG CTCTGCCAAA 
GCCCCCCTTC CTCCCACAGC GAAACCAGCC CGTCAGAACC CTGGAACAGC CAAAGCTCCT 
CCTTCCACTG CCAAGCCAGT GCTTCCCGCC GGGCGGTCCT CCCTTCCCGT TACCCCCATG 
ACAGCGTCCC AGGCTGCTCG GGCCAACGTG GCCAAAAACC TCCCCTTTAC CAGCAAGCCG 
GCCAAGCTGA CCCACCCTTC CATTAAGTCA TCCTTGCCAG CCTCAGGTCC GAGCCTAACC 
CAGACCCCTC GGCCCCCAGC AAAGCCGGTG CTTCCTACCG CGGGGATCAA ATTGTCCAGA 
ACAATCCCTC CCACCTTCTC CCCAGGCTCT CCTCCTGGGC TCAACTCCAC TGGTGACAGA 
AAAGTGGTTG AGCCCGCTGT GAAGAAGGAA TTGACTCCAA AGAGCACCCC CCAGCCACCC 
AGCCATCCCA GCCCTAGGAG GACTGGAGCA GTCCCTAGTA CCGTCTCCTT CCCTTCAGGC 
CTGAAGAACC TGACCACGTG GCCCAGCCCC AAACAGGCTC AGCTCAGCCG TACAGCTGCC 
CCGGTTCTCC CACAGCACCC AGCCTCCAGC TCCCGTTTTG TGGGCGCCGT CACTCTCACT 
CACACAGATG GATACCTGTT CTTTGATCCA ATTGGGCCCA CCTTGTTCCC ACTGCTGTTG 
GGCCCTCCAG GGCAGAAGGG AGACCCTGGC CTCCCGGGCC CCCCTGGCTT GCCTGGGTTG 
CCTGGAATAT CAGGCAGAAG AGGACCCCGG GGTCCCCCTG GGCCTCATGG AAACCCTGGG 
CCTCCTGGCT CTCCTGGTGC AAAGGGACAA AAAGGAGATC CTGGGCTTTC CCCTGGAAAG 
GCCTATGATG GTGACAAGGG AGACGTAGGC TTACCAGGAC TGACTGGGAT TCCGGGACCA 
ACTGGCCGGA AGGGGCTGAA AGGCTATCCT GGACCACCTG GGCATCCTGG AGACCAGGGA 
GAGCCAGGAC CAGAAGGGAG TCTGGGAGCC AAAGGTTATC CTGGCAGGCA GGGATTACCT 
GGGCCAATAG GAGAACCTGG ACCCAAAGGC AGTCGGGGGT ATATTGGTCT CCCCGGGCTC 
TTTGGCCTGC CAGGACTTGA TGGAGAAAGG GGGCCTCCTG GAGCTCATGG CAAGAGGGGC 
AAGATGGGTA GGCCGGGATT TCCTGGTGAC TTTGGGGAAA GAGGGCCCCC CGGATTGGAT 
GGCAACCCCG GAGAGCTGGG TGCCCCAGGC CCACCTGGAG TTCATGGACT TATCGGTGAC 
ATGGGAGCTA TTGGTCCAAT TGGCTACCCA GGGCCCAAGG GAATGAAGGG GCTTATGGGA 
AACATCGGAG AACACGGACT GAAAGGTGAT AAGGGCGAGC AAGGGCTGCC TGGTGTGCCT 
GGAGATATCG GATTCCAAGG AGATAAGGGG AGTCAGGGAA TATCAGGGTT ACCGGGTGCT 
CGAGGAAAGC CAGGTCCTAC AGGCAAGGTT GGTGACAAAG GACCAGTTGG GTTTCCTGGG 
CCTCCAGGTC CTGAGGGATT CCCAGGGGAC ATAGGACCAC CAGGCCAGAA TGGTCCAGAA 
GGTGCGAAGG GCAAGCCAGG AGCCCGAGGC TTACCAGGAC CGAGAGGACA GCTTGGGCAG 
GAGGGAGATG AGGGACCAAC GGGACCACCA GGATCCCCTG GCTTAGAGGG TCAACCTGGC 
CGGAAAGGAT TTCCAGGAAG GCCCGGCCCT GATGGCCCCA AGGGTGAGCC AGGAAACTCG 
GGTCGGCCTG GAAGAATGGG TGAGCAGGGC CTGATGGGAT TTATCGGTCT GGTCGGGGAG 
CCAGGAATCG TTGGAGAGAA GGGTAGCCGA GGTGCTATGG GACCACCAGG TGCTCCTGGA 
GCCAAGGGGT CGATGGGTCA TCCTGGTTCA GCAGGTGGAG TTGGAGATCC TGGAGACCCC 
GGGCCCCCAG GACCTCCTGG ACCTCGTGGG CCACTGGGGA TGCGAGGAAC AAAAGGACGT 
CGGGGGCCCC GAGGACCAGA TGGACCAGCA GGGGAGCCAG GGTCAAAAGG TGTCAAGGGC 
CCTCCAGGAA CACAGGGCAA ACAGGGCCTG GATGGACAGC AGGGCCAGGA AGGCGAGAGA 
GGGGAGCTGG GGCCTCGTGG CTTCCCGGGA ATCCCGGGTC CTTCCGGCCC CCCGGGGGCT 
AAGGGCGTTC CAGGAGAAGC AGGCCCTCAA GGACCACAGG GACTGATTGG CCCATTGGGA 
GAGATTGGAC CTAAGGGACC CCCTGGTGCT TTGGGAGAGC CTGGCCTTCC AGGTGAAGCT 
GGGATGAAGG GCGATTTTGG ACCTTTGGGC ATCCCTGGAG AGCAAGGTCT GGTTGGCCAG 
CGGGGAGAGA CAGGGCTGGA AGGAGATAGT GGCCCAGCTG GACCTGATGG TTTGAAGGGA 
GACAAGGGTG AGCCAGGCCC AGATGGCGAG CGAGGAGAGA AAGGCAAAGA AGGTCTGAAG 
GGCAAAGAAG GTCCCCCTGG CTATCCTGGG ATCACAGGTG TCCGGGGTCC AGAAGGAAAA 
CCAGGCAAAC AAGGCGAGAA GGGACGAACC GGACCTAAGG GTGCCAAGGG CTACCAAGGG 
CATCTTGGTG AGATGGGTGT TCCAGGAGAG GCCGGGCCAG CTGGCAACCA AGGCCCAAAA 
GGATCCCGAG GGACCCTGGG ACCAACGGGT GCTCCAGGAA GAATGGGAGC TCAAGGAGAA 
CCTGGATTGC CTGGTTATGA TGGACATAAA GGACTATCAG GACCCATTGG ACCTCCCGGG 
CCAAAAGGCG AGAAGGGAGA ACAGGGTGAT GATGGGAAGA TTGAGGGCCC TCCAGGGCCT 
CCTGGAGATC GGGGTCCAGT GGGTGATCGT GGAGACCGAG GGGAGCCTGG AGATCCTGGA 
TACCCTGGCC AGGAGGGTTT TGAAGGAAGC AGAGGAAAAG CTGGTCAGCA AGGCTTACCG 
GGACATCCTG GACCCCGGGG ACGACCAGGA CCCAAAGGAT CAAAGGGTGA AGAGGGTCAG 
AAAGGGAAAC AAGGAAAGCG TGGAGAAGCT GGCAGTCGAG GAATGCAGGG CCTGGTGGGG 
CAGCCAGGGC CTCGAGGTGT GGTAGGAAGA CAAGGCCAAG AAGGACCCCC AGGTCAGGAT 
GGAATTCCTG GGAAGGATGG TCGAGCTGGA CATCAGGGGG AACAAGGAGA TGATGGAGAA 
GTTGGACCCA TTGGCCCTGC AGGAAGGAGA GGAAATCCAG GTGTGGCTGG TTTGCCTGGA 
GCTCAGGGAC CCCCAGGATT CAAGGGTGAA AGTGGGTTGC CTGGACAACT GGGCCCTACT 
GGCAAGCGAG GGACAGAGGG AGGAACAGGG TTCCCTGGGA GCCAAGGGGA GCCAGGATCC 
AAAGGCCAGC CGGGTGACTT TGGAGAAATG GGCTTTCCTG GGATTGCTGG CCTCTTTGGA 
CCCAAGGGCC CTCCAGGAGA TATTGGTTTC AAAGGAATCC AGGGGCCCCG GGGACCACCA 
GGTTTAATGG GAAAAGAAGG TGTCATTGGC CCCTTGGGGA TCCTAGGACC GTCTGGAATT 
CCGGGTCCAA AAGGGAACAA AGGAAGCCGA GGAGATTTGG GATTGCAAGG CCCAAGGGGT 
CCTCCTGGCC CCAGAGGACG TCCTGGCCCA CCGGGCCCTC CTGGGATCCC CATCTCATTT 
CAGCAAGATG ACCTCGGAGC AGCTTTTCAG ACGTGGATGA ACTCCAACAG TGCACTTAGG 
ACAGAGGGCT ACTCCCATTC AGACCTCCTA GTGTTGGACC AGGGTGGGGA GATCTTTAAG 
ACCTTACACT ACCTCAGCAG CCTCATCCAG AGCATCAAGA AGCCTCTAGG AACCAAGGAG 
ACCCCAGCCC GTATCTGTCG GGACCTTATG AACTGTGAGC AGAAGATGGC CGATGGTACA 
TATTGGATCG ACCCAAATCT TGGCTGTGCT TCAGACACCA TCGAAGTCAC CTGCAATTTT 
ACTAAAGGTG GACAGACATG TCTCAGGCCC ATCACTGCTT CAAAGGTGGA CTTTGCCATT 
GGCCGGGTCC AGATGAATTT TCTTCACCTG CTGAGCTCGG AGGTGATCCA GCACATCACT 
ATCCACTGCC TCAACATGTC TGTATGGCAG GAGGGGACAG GCAAGGTGTC CCCCAAACAA 
GCCGTGCGCT TCCGAGCCTG GAATGGGCAG ATCTTTGAAG CGGGCGGCCA GTTTGAGCCA 
GAGGTGTCCT CAGATGATTG CAAGGTTCAG GATGGCCGCT GGCACCAGAC CCTGTTCATT 
TTCCGGACCC AAGACCCTCA CCAGCTGCCC ATCGTCAGCG TCTACAACCT CCCTCCAGCC 
ACCCCTGGGA GGCAATACCG CCTGGAAGTT GGACCTGCGT GCTTCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003757400.1
CDS	1..5751
Translation	MILKKDPWCQWAGGNTLLPPPITYQWLSVIRGFLLAWIAFCSTCGLAITQGDPEDVDVLQRLGLGGKKLPGGRAPGLAPIPPGVIPFQSGFIFTQRARIEALTGAIIPTTFGTDLTLVLSLCSHRVNNAFLFAVRSRRKKLQLGLQFIPGKIVLFLGQRRSLAFSYDVHDGRWHHLAISLRGQGATLLTSCGKHRVPMDLHFKKEDSLDPEGSFLFGKMNQHSVQFEGAVCQFNIYPSAKAAHNYCKYLRKQCRQADTYRPLLPPLLPLLPQDSEAHTHLGLQPSTSLQSLRNLTTAMPSLAPLRMAKTTPALPRAPGQGARATWVPSAPAGLGAPPHPPVRTSAADPRASRSPTVGTLVWTLPATAKPLPFSSGKASSPGLRPALITPQSNLTKSPVRAMKPMPPSKASVVKTPLVPSKPVTPSVTVVPAKRPHPLVKPVLTSPAKKPVPSGSQPGSAKAPLPPTAKPARQNPGTAKAPPSTAKPVLPAGRSSLPVTPMTASQAARANVAKNLPFTSKPAKLTHPSIKSSLPASGPSLTQTPRPPAKPVLPTAGIKLSRTIPPTFSPGSPPGLNSTGDRKVVEPAVKKELTPKSTPQPPSHPSPRRTGAVPSTVSFPSGLKNLTTWPSPKQAQLSRTAAPVLPQHPASSSRFVGAVTLTHTDGYLFFDPIGPTLFPLLLGPPGQKGDPGLPGPPGLPGLPGISGRRGPRGPPGPHGNPGPPGSPGAKGQKGDPGLSPGKAYDGDKGDVGLPGLTGIPGPTGRKGLKGYPGPPGHPGDQGEPGPEGSLGAKGYPGRQGLPGPIGEPGPKGSRGYIGLPGLFGLPGLDGERGPPGAHGKRGKMGRPGFPGDFGERGPPGLDGNPGELGAPGPPGVHGLIGDMGAIGPIGYPGPKGMKGLMGNIGEHGLKGDKGEQGLPGVPGDIGFQGDKGSQGISGLPGARGKPGPTGKVGDKGPVGFPGPPGPEGFPGDIGPPGQNGPEGAKGKPGARGLPGPRGQLGQEGDEGPTGPPGSPGLEGQPGRKGFPGRPGPDGPKGEPGNSGRPGRMGEQGLMGFIGLVGEPGIVGEKGSRGAMGPPGAPGAKGSMGHPGSAGGVGDPGDPGPPGPPGPRGPLGMRGTKGRRGPRGPDGPAGEPGSKGVKGPPGTQGKQGLDGQQGQEGERGELGPRGFPGIPGPSGPPGAKGVPGEAGPQGPQGLIGPLGEIGPKGPPGALGEPGLPGEAGMKGDFGPLGIPGEQGLVGQRGETGLEGDSGPAGPDGLKGDKGEPGPDGERGEKGKEGLKGKEGPPGYPGITGVRGPEGKPGKQGEKGRTGPKGAKGYQGHLGEMGVPGEAGPAGNQGPKGSRGTLGPTGAPGRMGAQGEPGLPGYDGHKGLSGPIGPPGPKGEKGEQGDDGKIEGPPGPPGDRGPVGDRGDRGEPGDPGYPGQEGFEGSRGKAGQQGLPGHPGPRGRPGPKGSKGEEGQKGKQGKRGEAGSRGMQGLVGQPGPRGVVGRQGQEGPPGQDGIPGKDGRAGHQGEQGDDGEVGPIGPAGRRGNPGVAGLPGAQGPPGFKGESGLPGQLGPTGKRGTEGGTGFPGSQGEPGSKGQPGDFGEMGFPGIAGLFGPKGPPGDIGFKGIQGPRGPPGLMGKEGVIGPLGILGPSGIPGPKGNKGSRGDLGLQGPRGPPGPRGRPGPPGPPGIPISFQQDDLGAAFQTWMNSNSALRTEGYSHSDLLVLDQGGEIFKTLHYLSSLIQSIKKPLGTKETPARICRDLMNCEQKMADGTYWIDPNLGCASDTIEVTCNFTKGGQTCLRPITASKVDFAIGRVQMNFLHLLSSEVIQHITIHCLNMSVWQEGTGKVSPKQAVRFRAWNGQIFEAGGQFEPEVSSDDCKVQDGRWHQTLFIFRTQDPHQLPIVSVYNLPPATPGRQYRLEVGPACFL

Target ORF information:

RefSeq Version	XM_003757352.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii collagen, type XXVII, alpha 1 (COL27A1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003757352.1

ORF Insert Sequence:

ATGATACTGA AGAAGGACCC ATGGTGTCAA TGGGCAGGTG GCAACACTTT GCTGCCACCA 
CCAATCACTT ATCAATGGTT ATCAGTGATC AGGGGATTCC TCCTAGCCTG GATTGCCTTC 
TGCTCTACCT GTGGCCTGGC CATCACCCAA GGAGATCCTG AAGATGTGGA TGTCCTGCAA 
CGGCTGGGCC TCGGGGGGAA GAAGTTGCCC GGCGGCCGGG CCCCAGGGCT GGCCCCCATC 
CCCCCAGGAG TCATCCCGTT CCAGTCGGGG TTCATCTTCA CCCAGCGGGC CCGGATTGAA 
GCCCTTACAG GCGCCATCAT TCCCACCACC TTCGGCACTG ACCTGACCCT GGTGCTGAGC 
CTGTGCTCCC ACCGAGTCAA CAACGCCTTC CTCTTTGCTG TCCGGAGCCG CAGGAAGAAG 
CTGCAGCTGG GGCTCCAGTT CATCCCTGGC AAGATCGTGC TGTTCCTGGG CCAGCGGCGC 
TCCCTGGCCT TCAGCTACGA CGTGCATGAC GGGCGATGGC ACCACTTGGC CATCAGTCTC 
CGAGGCCAGG GGGCCACCCT CCTCACCTCC TGCGGGAAGC ACCGCGTGCC CATGGATCTG 
CATTTTAAGA AAGAGGACTC TCTTGATCCC GAGGGGTCTT TCCTCTTTGG GAAGATGAAT 
CAGCACTCAG TCCAATTTGA GGGCGCCGTA TGCCAGTTCA ATATTTATCC TTCTGCCAAG 
GCAGCTCACA ATTATTGTAA ATATCTGAGG AAGCAGTGCA GACAGGCCGA CACCTACCGG 
CCACTGCTGC CCCCTCTTCT TCCCCTCCTT CCCCAGGACT CCGAGGCCCA TACCCATCTT 
GGCCTCCAGC CCAGCACCTC TCTGCAGAGC CTGAGGAATC TGACCACTGC GATGCCTTCG 
CTGGCACCCC TTAGAATGGC CAAGACCACC CCGGCCTTGC CCAGAGCCCC CGGCCAAGGT 
GCCAGGGCTA CATGGGTGCC CTCAGCACCG GCCGGGCTTG GGGCACCCCC CCACCCGCCT 
GTGAGGACTA GTGCCGCGGA TCCCCGGGCG TCCCGATCCC CCACAGTGGG TACTTTGGTC 
TGGACCCTAC CAGCAACTGC AAAGCCATTG CCTTTCTCTT CAGGCAAAGC TTCCTCACCT 
GGGCTCAGGC CTGCACTGAT CACCCCTCAG AGCAACTTGA CCAAATCTCC CGTTCGCGCC 
ATGAAGCCCA TGCCCCCATC CAAGGCCAGT GTGGTTAAAA CTCCCCTTGT TCCCAGTAAG 
CCAGTAACTC CTTCAGTCAC TGTGGTCCCA GCTAAGAGGC CCCATCCCCT GGTCAAGCCG 
GTGCTGACCT CCCCTGCTAA GAAGCCAGTG CCCTCTGGCT CTCAGCCTGG CTCTGCCAAA 
GCCCCCCTTC CTCCCACAGC GAAACCAGCC CGTCAGAACC CTGGAACAGC CAAAGCTCCT 
CCTTCCACTG CCAAGCCAGT GCTTCCCGCC GGGCGGTCCT CCCTTCCCGT TACCCCCATG 
ACAGCGTCCC AGGCTGCTCG GGCCAACGTG GCCAAAAACC TCCCCTTTAC CAGCAAGCCG 
GCCAAGCTGA CCCACCCTTC CATTAAGTCA TCCTTGCCAG CCTCAGGTCC GAGCCTAACC 
CAGACCCCTC GGCCCCCAGC AAAGCCGGTG CTTCCTACCG CGGGGATCAA ATTGTCCAGA 
ACAATCCCTC CCACCTTCTC CCCAGGCTCT CCTCCTGGGC TCAACTCCAC TGGTGACAGA 
AAAGTGGTTG AGCCCGCTGT GAAGAAGGAA TTGACTCCAA AGAGCACCCC CCAGCCACCC 
AGCCATCCCA GCCCTAGGAG GACTGGAGCA GTCCCTAGTA CCGTCTCCTT CCCTTCAGGC 
CTGAAGAACC TGACCACGTG GCCCAGCCCC AAACAGGCTC AGCTCAGCCG TACAGCTGCC 
CCGGTTCTCC CACAGCACCC AGCCTCCAGC TCCCGTTTTG TGGGCGCCGT CACTCTCACT 
CACACAGATG GATACCTGTT CTTTGATCCA ATTGGGCCCA CCTTGTTCCC ACTGCTGTTG 
GGCCCTCCAG GGCAGAAGGG AGACCCTGGC CTCCCGGGCC CCCCTGGCTT GCCTGGGTTG 
CCTGGAATAT CAGGCAGAAG AGGACCCCGG GGTCCCCCTG GGCCTCATGG AAACCCTGGG 
CCTCCTGGCT CTCCTGGTGC AAAGGGACAA AAAGGAGATC CTGGGCTTTC CCCTGGAAAG 
GCCTATGATG GTGACAAGGG AGACGTAGGC TTACCAGGAC TGACTGGGAT TCCGGGACCA 
ACTGGCCGGA AGGGGCTGAA AGGCTATCCT GGACCACCTG GGCATCCTGG AGACCAGGGA 
GAGCCAGGAC CAGAAGGGAG TCTGGGAGCC AAAGGTTATC CTGGCAGGCA GGGATTACCT 
GGGCCAATAG GAGAACCTGG ACCCAAAGGC AGTCGGGGGT ATATTGGTCT CCCCGGGCTC 
TTTGGCCTGC CAGGACTTGA TGGAGAAAGG GGGCCTCCTG GAGCTCATGG CAAGAGGGGC 
AAGATGGGTA GGCCGGGATT TCCTGGTGAC TTTGGGGAAA GAGGGCCCCC CGGATTGGAT 
GGCAACCCCG GAGAGCTGGG TGCCCCAGGC CCACCTGGAG TTCATGGACT TATCGGTGAC 
ATGGGAGCTA TTGGTCCAAT TGGCTACCCA GGGCCCAAGG GAATGAAGGG GCTTATGGGA 
AACATCGGAG AACACGGACT GAAAGGTGAT AAGGGCGAGC AAGGGCTGCC TGGTGTGCCT 
GGAGATATCG GATTCCAAGG AGATAAGGGG AGTCAGGGAA TATCAGGGTT ACCGGGTGCT 
CGAGGAAAGC CAGGTCCTAC AGGCAAGGTT GGTGACAAAG GACCAGTTGG GTTTCCTGGG 
CCTCCAGGTC CTGAGGGATT CCCAGGGGAC ATAGGACCAC CAGGCCAGAA TGGTCCAGAA 
GGTGCGAAGG GCAAGCCAGG AGCCCGAGGC TTACCAGGAC CGAGAGGACA GCTTGGGCAG 
GAGGGAGATG AGGGACCAAC GGGACCACCA GGATCCCCTG GCTTAGAGGG TCAACCTGGC 
CGGAAAGGAT TTCCAGGAAG GCCCGGCCCT GATGGCCCCA AGGGTGAGCC AGGAAACTCG 
GGTCGGCCTG GAAGAATGGG TGAGCAGGGC CTGATGGGAT TTATCGGTCT GGTCGGGGAG 
CCAGGAATCG TTGGAGAGAA GGGTAGCCGA GGTGCTATGG GACCACCAGG TGCTCCTGGA 
GCCAAGGGGT CGATGGGTCA TCCTGGTTCA GCAGGTGGAG TTGGAGATCC TGGAGACCCC 
GGGCCCCCAG GACCTCCTGG ACCTCGTGGG CCACTGGGGA TGCGAGGAAC AAAAGGACGT 
CGGGGGCCCC GAGGACCAGA TGGACCAGCA GGGGAGCCAG GGTCAAAAGG TGTCAAGGGC 
CCTCCAGGAA CACAGGGCAA ACAGGGCCTG GATGGACAGC AGGGCCAGGA AGGCGAGAGA 
GGGGAGCTGG GGCCTCGTGG CTTCCCGGGA ATCCCGGGTC CTTCCGGCCC CCCGGGGGCT 
AAGGGCGTTC CAGGAGAAGC AGGCCCTCAA GGACCACAGG GACTGATTGG CCCATTGGGA 
GAGATTGGAC CTAAGGGACC CCCTGGTGCT TTGGGAGAGC CTGGCCTTCC AGGTGAAGCT 
GGGATGAAGG GCGATTTTGG ACCTTTGGGC ATCCCTGGAG AGCAAGGTCT GGTTGGCCAG 
CGGGGAGAGA CAGGGCTGGA AGGAGATAGT GGCCCAGCTG GACCTGATGG TTTGAAGGGA 
GACAAGGGTG AGCCAGGCCC AGATGGCGAG CGAGGAGAGA AAGGCAAAGA AGGTCTGAAG 
GGCAAAGAAG GTCCCCCTGG CTATCCTGGG ATCACAGGTG TCCGGGGTCC AGAAGGAAAA 
CCAGGCAAAC AAGGCGAGAA GGGACGAACC GGACCTAAGG GTGCCAAGGG CTACCAAGGG 
CATCTTGGTG AGATGGGTGT TCCAGGAGAG GCCGGGCCAG CTGGCAACCA AGGCCCAAAA 
GGATCCCGAG GGACCCTGGG ACCAACGGGT GCTCCAGGAA GAATGGGAGC TCAAGGAGAA 
CCTGGATTGC CTGGTTATGA TGGACATAAA GGACTATCAG GACCCATTGG ACCTCCCGGG 
CCAAAAGGCG AGAAGGGAGA ACAGGGTGAT GATGGGAAGA TTGAGGGCCC TCCAGGGCCT 
CCTGGAGATC GGGGTCCAGT GGGTGATCGT GGAGACCGAG GGGAGCCTGG AGATCCTGGA 
TACCCTGGCC AGGAGGGTTT TGAAGGAAGC AGAGGAAAAG CTGGTCAGCA AGGCTTACCG 
GGACATCCTG GACCCCGGGG ACGACCAGGA CCCAAAGGAT CAAAGGGTGA AGAGGGTCAG 
AAAGGGAAAC AAGGAAAGCG TGGAGAAGCT GGCAGTCGAG GAATGCAGGG CCTGGTGGGG 
CAGCCAGGGC CTCGAGGTGT GGTAGGAAGA CAAGGCCAAG AAGGACCCCC AGGTCAGGAT 
GGAATTCCTG GGAAGGATGG TCGAGCTGGA CATCAGGGGG AACAAGGAGA TGATGGAGAA 
GTTGGACCCA TTGGCCCTGC AGGAAGGAGA GGAAATCCAG GTGTGGCTGG TTTGCCTGGA 
GCTCAGGGAC CCCCAGGATT CAAGGGTGAA AGTGGGTTGC CTGGACAACT GGGCCCTACT 
GGCAAGCGAG GGACAGAGGG AGGAACAGGG TTCCCTGGGA GCCAAGGGGA GCCAGGATCC 
AAAGGCCAGC CGGGTGACTT TGGAGAAATG GGCTTTCCTG GGATTGCTGG CCTCTTTGGA 
CCCAAGGGCC CTCCAGGAGA TATTGGTTTC AAAGGAATCC AGGGGCCCCG GGGACCACCA 
GGTTTAATGG GAAAAGAAGG TGTCATTGGC CCCTTGGGGA TCCTAGGACC GTCTGGAATT 
CCGGGTCCAA AAGGGAACAA AGGAAGCCGA GGAGATTTGG GATTGCAAGG CCCAAGGGGT 
CCTCCTGGCC CCAGAGGACG TCCTGGCCCA CCGGGCCCTC CTGGGATCCC CATCTCATTT 
CAGCAAGATG ACCTCGGAGC AGCTTTTCAG ACGTGGATGA ACTCCAACAG TGCACTTAGG 
ACAGAGGGCT ACTCCCATTC AGACCTCCTA GTGTTGGACC AGGGTGGGGA GATCTTTAAG 
ACCTTACACT ACCTCAGCAG CCTCATCCAG AGCATCAAGA AGCCTCTAGG AACCAAGGAG 
ACCCCAGCCC GTATCTGTCG GGACCTTATG AACTGTGAGC AGAAGATGGC CGATGGTACA 
TATTGGATCG ACCCAAATCT TGGCTGTGCT TCAGACACCA TCGAAGTCAC CTGCAATTTT 
ACTAAAGGTG GACAGACATG TCTCAGGCCC ATCACTGCTT CAAAGGTGGA CTTTGCCATT 
GGCCGGGTCC AGATGAATTT TCTTCACCTG CTGAGCTCGG AGGTGATCCA GCACATCACT 
ATCCACTGCC TCAACATGTC TGTATGGCAG GAGGGGACAG GCAAGGTGTC CCCCAAACAA 
GCCGTGCGCT TCCGAGCCTG GAATGGGCAG ATCTTTGAAG CGGGCGGCCA GTTTGAGCCA 
GAGGTGTCCT CAGATGATTG CAAGGTTCAG GATGGCCGCT GGCACCAGAC CCTGTTCATT 
TTCCGGACCC AAGACCCTCA CCAGCTGCCC ATCGTCAGCG TCTACAACCT CCCTCCAGCC 
ACCCCTGGGA GGCAATACCG CCTGGAAGTT GGACCTGCGT GCTTCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL27A1 ( XM_023494250.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_023350018.1 Sarcophilus harrisii collagen type XXVII alpha 1 chain (COL27A1), transcript variant X1, mRNA.

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CloneID	OSa150306
Clone ID Related Accession (Same CDS sequence)	XM_023494250.1 , XM_023494250.2
Accession Version	XM_023494250.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5712bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515513600000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	collagen alpha-1(XXVII) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003816661.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCGG AATCCGACCG GGTCTCCAGA GTCAGAGCAG CTGCGTCCAG GGGGGGATTC 
CTCCTAGCCT GGATTGCCTT CTGCTCTACC TGTGGCCTGG CCATCACCCA AGGAGATCCT 
GAAGATGTGG ATGTCCTGCA ACGGCTGGGC CTCGGGGGGA AGAAGTTGCC CGGCGGCCGG 
GCCCCAGGGC TGGCCCCCAT CCCCCCAGGA GTCATCCCGT TCCAGTCGGG GTTCATCTTC 
ACCCAGCGGG CCCGGATTGA AGCCCTTACA GGCGCCATCA TTCCCACCAC CTTCGGCACT 
GACCTGACCC TGGTGCTGAG CCTGTGCTCC CACCGAGTCA ACAACGCCTT CCTCTTTGCT 
GTCCGGAGCC GCAGGAAGAA GCTGCAGCTG GGGCTCCAGT TCATCCCTGG CAAGATCGTG 
CTGTTCCTGG GCCAGCGGCG CTCCCTGGCC TTCAGCTACG ACGTGCATGA CGGGCGATGG 
CACCACTTGG CCATCAGTCT CCGAGGCCAG GGGGCCACCC TCCTCACCTC CTGCGGGAAG 
CACCGCGTGC CCATGGATCT GCATTTTAAG AAAGAGGACT CTCTTGATCC CGAGGGGTCT 
TTCCTCTTTG GGAAGATGAA TCAGCACTCA GTCCAATTTG AGGGCGCCGT ATGCCAGTTC 
AATATTTATC CTTCTGCCAA GGCAGCTCAC AATTATTGTA AATATCTGAG GAAGCAGTGC 
AGACAGGCCG ACACCTACCG GCCACTGCTG CCCCCTCTTC TTCCCCTCCT TCCCCAGGAC 
TCCGAGGCCC ATACCCATCT TGGCCTCCAG CCCAGCACCT CTCTGCAGAG CCTGAGGAAT 
CTGACCACTG CGATGCCTTC GCTGGCACCC CTTAGAATGG CCAAGACCAC CCCGGCCTTG 
CCCAGAGCCC CCGGCCAAGG TGCCAGGGCT ACATGGGTGC CCTCAGCACC GGCCGGGCTT 
GGGGCACCCC CCCACCCGCC TGTGAGGACT AGTGCCGCGG ATCCCCGGGC GTCCCGATCC 
CCCACAGTGG GTACTTTGGT CTGGACCCTA CCAGCAACTG CAAAGCCATT GCCTTTCTCT 
TCAGGCAAAG CTTCCTCACC TGGGCTCAGG CCTGCACTGA TCACCCCTCA GAGCAACTTG 
ACCAAATCTC CCGTTCGCGC CATGAAGCCC ATGCCCCCAT CCAAGGCCAG TGTGGTTAAA 
ACTCCCCTTG TTCCCAGTAA GCCAGTAACT CCTTCAGTCA CTGTGGTCCC AGCTAAGAGG 
CCCCATCCCC TGGTCAAGCC GGTGCTGACC TCCCCTGCTA AGAAGCCAGT GCCCTCTGGC 
TCTCAGCCTG GCTCTGCCAA AGCCCCCCTT CCTCCCACAG CGAAACCAGC CCGTCAGAAC 
CCTGGAACAG CCAAAGCTCC TCCTTCCACT GCCAAGCCAG TGCTTCCCGC CGGGCGGTCC 
TCCCTTCCCG TTACCCCCAT GACAGCGTCC CAGGCTGCTC GGGCCAACGT GGCCAAAAAC 
CTCCCCTTTA CCAGCAAGCC GGCCAAGCTG ACCCACCCTT CCATTAAGTC ATCCTTGCCA 
GCCTCAGGTC CGAGCCTAAC CCAGACCCCT CGGCCCCCAG CAAAGCCGGT GCTTCCTACC 
GCGGGGATCA AATTGTCCAG AACAATCCCT CCCACCTTCT CCCCAGGCTC TCCTCCTGGG 
CTCAACTCCA CTGGTGACAG AAAAGTGGTT GAGCCCGCTG TGAAGAAGGA ATTGACTCCA 
AAGAGCACCC CCCAGCCACC CAGCCATCCC AGCCCTAGGA GGACTGGAGC AGTCCCTAGT 
ACCGTCTCCT TCCCTTCAGG CCTGAAGAAC CTGACCACGT GGCCCAGCCC CAAACAGGCT 
CAGCTCAGCC GTACAGCTGC CCCGGTTCTC CCACAGCACC CAGCCTCCAG CTCCCGTTTT 
GTGGGCGCCG TCACTCTCAC TCACACAGAT GGATACCTGT TCTTTGATCC AATTGGGCCC 
ACCTTGTTCC CACTGCTGTT GGGCCCTCCA GGGCAGAAGG GAGACCCTGG CCTCCCGGGC 
CCCCCTGGCT TGCCTGGGTT GCCTGGAATA TCAGGCAGAA GAGGACCCCG GGGTCCCCCT 
GGGCCTCATG GAAACCCTGG GCCTCCTGGC TCTCCTGGTG CAAAGGGACA AAAAGGAGAT 
CCTGGGCTTT CCCCTGGAAA GGCCTATGAT GGTGACAAGG GAGACGTAGG CTTACCAGGA 
CTGACTGGGA TTCCGGGACC AACTGGCCGG AAGGGGCTGA AAGGCTATCC TGGACCACCT 
GGGCATCCTG GAGACCAGGG AGAGCCAGGA CCAGAAGGGA GTCTGGGAGC CAAAGGTTAT 
CCTGGCAGGC AGGGATTACC TGGGCCAATA GGAGAACCTG GACCCAAAGG CAGTCGGGGG 
TATATTGGTC TCCCCGGGCT CTTTGGCCTG CCAGGACTTG ATGGAGAAAG GGGGCCTCCT 
GGAGCTCATG GCAAGAGGGG CAAGATGGGT AGGCCGGGAT TTCCTGGTGA CTTTGGGGAA 
AGAGGGCCCC CCGGATTGGA TGGCAACCCC GGAGAGCTGG GTGCCCCAGG CCCACCTGGA 
GTTCATGGAC TTATCGGTGA CATGGGAGCT ATTGGTCCAA TTGGCTACCC AGGGCCCAAG 
GGAATGAAGG GGCTTATGGG AAACATCGGA GAACACGGAC TGAAAGGTGA TAAGGGCGAG 
CAAGGGCTGC CTGGTGTGCC TGGAGATATC GGATTCCAAG GAGATAAGGG GAGTCAGGGA 
ATATCAGGGT TACCGGGTGC TCGAGGAAAG CCAGGTCCTA CAGGCAAGGT TGGTGACAAA 
GGACCAGTTG GGTTTCCTGG GCCTCCAGGT CCTGAGGGAT TCCCAGGGGA CATAGGACCA 
CCAGGCCAGA ATGGTCCAGA AGGTGCGAAG GGCAAGCCAG GAGCCCGAGG CTTACCAGGA 
CCGAGAGGAC AGCTTGGGCA GGAGGGAGAT GAGGGACCAA CGGGACCACC AGGATCCCCT 
GGCTTAGAGG GTCAACCTGG CCGGAAAGGA TTTCCAGGAA GGCCCGGCCC TGATGGCCCC 
AAGGGTGAGC CAGGAAACTC GGGTCGGCCT GGAAGAATGG GTGAGCAGGG CCTGATGGGA 
TTTATCGGTC TGGTCGGGGA GCCAGGAATC GTTGGAGAGA AGGGTAGCCG AGGTGCTATG 
GGACCACCAG GTGCTCCTGG AGCCAAGGGG TCGATGGGTC ATCCTGGTTC AGCAGGTGGA 
GTTGGAGATC CTGGAGACCC CGGGCCCCCA GGACCTCCTG GACCTCGTGG GCCACTGGGG 
ATGCGAGGAA CAAAAGGACG TCGGGGGCCC CGAGGACCAG ATGGACCAGC AGGGGAGCCA 
GGGTCAAAAG GTGTCAAGGG CCCTCCAGGA ACACAGGGCA AACAGGGCCT GGATGGACAG 
CAGGGCCAGG AAGGCGAGAG AGGGGAGCTG GGGCCTCGTG GCTTCCCGGG AATCCCGGGT 
CCTTCCGGCC CCCCGGGGGC TAAGGGCGTT CCAGGAGAAG CAGGCCCTCA AGGACCACAG 
GGACTGATTG GCCCATTGGG AGAGATTGGA CCTAAGGGAC CCCCTGGTGC TTTGGGAGAG 
CCTGGCCTTC CAGGTGAAGC TGGGATGAAG GGCGATTTTG GACCTTTGGG CATCCCTGGA 
GAGCAAGGTC TGGTTGGCCA GCGGGGAGAG ACAGGGCTGG AAGGAGATAG TGGCCCAGCT 
GGACCTGATG GTTTGAAGGG AGACAAGGGT GAGCCAGGCC CAGATGGCGA GCGAGGAGAG 
AAAGGCAAAG AAGGTCTGAA GGGCAAAGAA GGTCCCCCTG GCTATCCTGG GATCACAGGT 
GTCCGGGGTC CAGAAGGAAA ACCAGGCAAA CAAGGCGAGA AGGGACGAAC CGGACCTAAG 
GGTGCCAAGG GCTACCAAGG GCATCTTGGT GAGATGGGTG TTCCAGGAGA GGCCGGGCCA 
GCTGGCAACC AAGGCCCAAA AGGATCCCGA GGGACCCTGG GACCAACGGG TGCTCCAGGA 
AGAATGGGAG CTCAAGGAGA ACCTGGATTG CCTGGTTATG ATGGACATAA AGGACTATCA 
GGACCCATTG GACCTCCCGG GCCAAAAGGC GAGAAGGGAG AACAGGGTGA TGATGGGAAG 
ATTGAGGGCC CTCCAGGGCC TCCTGGAGAT CGGGGTCCAG TGGGTGATCG TGGAGACCGA 
GGGGAGCCTG GAGATCCTGG ATACCCTGGC CAGGAGGGTT TTGAAGGAAG CAGAGGAAAA 
GCTGGTCAGC AAGGCTTACC GGGACATCCT GGACCCCGGG GACGACCAGG ACCCAAAGGA 
TCAAAGGGTG AAGAGGGTCA GAAAGGGAAA CAAGGAAAGC GTGGAGAAGC TGGCAGTCGA 
GGAATGCAGG GCCTGGTGGG GCAGCCAGGG CCTCGAGGTG TGGTAGGAAG ACAAGGCCAA 
GAAGGACCCC CAGGTCAGGA TGGAATTCCT GGGAAGGATG GTCGAGCTGG ACATCAGGGG 
GAACAAGGAG ATGATGGAGA AGTTGGACCC ATTGGCCCTG CAGGAAGGAG AGGAAATCCA 
GGTGTGGCTG GTTTGCCTGG AGCTCAGGGA CCCCCAGGAT TCAAGGGTGA AAGTGGGTTG 
CCTGGACAAC TGGGCCCTAC TGGCAAGCGA GGGACAGAGG GAGGAACAGG GTTCCCTGGG 
AGCCAAGGGG AGCCAGGATC CAAAGGCCAG CCGGGTGACT TTGGAGAAAT GGGCTTTCCT 
GGGATTGCTG GCCTCTTTGG ACCCAAGGGC CCTCCAGGAG ATATTGGTTT CAAAGGAATC 
CAGGGGCCCC GGGGACCACC AGGTTTAATG GGAAAAGAAG GTGTCATTGG CCCCTTGGGG 
ATCCTAGGAC CGTCTGGAAT TCCGGGTCCA AAAGGGAACA AAGGAAGCCG AGGAGATTTG 
GGATTGCAAG GCCCAAGGGG TCCTCCTGGC CCCAGAGGAC GTCCTGGCCC ACCGGGCCCT 
CCTGGGATCC CCATCTCATT TCAGCAAGAT GACCTCGGAG CAGCTTTTCA GACGTGGATG 
AACTCCAACA GTGCACTTAG GACAGAGGGC TACTCCCATT CAGACCTCCT AGTGTTGGAC 
CAGGGTGGGG AGATCTTTAA GACCTTACAC TACCTCAGCA GCCTCATCCA GAGCATCAAG 
AAGCCTCTAG GAACCAAGGA GACCCCAGCC CGTATCTGTC GGGACCTTAT GAACTGTGAG 
CAGAAGATGG CCGATGGTAC ATATTGGATC GACCCAAATC TTGGCTGTGC TTCAGACACC 
ATCGAAGTCA CCTGCAATTT TACTAAAGGT GGACAGACAT GTCTCAGGCC CATCACTGCT 
TCAAAGGTGG ACTTTGCCAT TGGCCGGGTC CAGATGAATT TTCTTCACCT GCTGAGCTCG 
GAGGTGATCC AGCACATCAC TATCCACTGC CTCAACATGT CTGTATGGCA GGAGGGGACA 
GGCAAGGTGT CCCCCAAACA AGCCGTGCGC TTCCGAGCCT GGAATGGGCA GATCTTTGAA 
GCGGGCGGCC AGTTTGAGCC AGAGGTGTCC TCAGATGATT GCAAGGTTCA GGATGGCCGC 
TGGCACCAGA CCCTGTTCAT TTTCCGGACC CAAGACCCTC ACCAGCTGCC CATCGTCAGC 
GTCTACAACC TCCCTCCAGC CACCCCTGGG AGGCAATACC GCCTGGAAGT TGGACCTGCG 
TGCTTCCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023350018.1
CDS	47..5758
Translation	MEPESDRVSRVRAAASRGGFLLAWIAFCSTCGLAITQGDPEDVDVLQRLGLGGKKLPGGRAPGLAPIPPGVIPFQSGFIFTQRARIEALTGAIIPTTFGTDLTLVLSLCSHRVNNAFLFAVRSRRKKLQLGLQFIPGKIVLFLGQRRSLAFSYDVHDGRWHHLAISLRGQGATLLTSCGKHRVPMDLHFKKEDSLDPEGSFLFGKMNQHSVQFEGAVCQFNIYPSAKAAHNYCKYLRKQCRQADTYRPLLPPLLPLLPQDSEAHTHLGLQPSTSLQSLRNLTTAMPSLAPLRMAKTTPALPRAPGQGARATWVPSAPAGLGAPPHPPVRTSAADPRASRSPTVGTLVWTLPATAKPLPFSSGKASSPGLRPALITPQSNLTKSPVRAMKPMPPSKASVVKTPLVPSKPVTPSVTVVPAKRPHPLVKPVLTSPAKKPVPSGSQPGSAKAPLPPTAKPARQNPGTAKAPPSTAKPVLPAGRSSLPVTPMTASQAARANVAKNLPFTSKPAKLTHPSIKSSLPASGPSLTQTPRPPAKPVLPTAGIKLSRTIPPTFSPGSPPGLNSTGDRKVVEPAVKKELTPKSTPQPPSHPSPRRTGAVPSTVSFPSGLKNLTTWPSPKQAQLSRTAAPVLPQHPASSSRFVGAVTLTHTDGYLFFDPIGPTLFPLLLGPPGQKGDPGLPGPPGLPGLPGISGRRGPRGPPGPHGNPGPPGSPGAKGQKGDPGLSPGKAYDGDKGDVGLPGLTGIPGPTGRKGLKGYPGPPGHPGDQGEPGPEGSLGAKGYPGRQGLPGPIGEPGPKGSRGYIGLPGLFGLPGLDGERGPPGAHGKRGKMGRPGFPGDFGERGPPGLDGNPGELGAPGPPGVHGLIGDMGAIGPIGYPGPKGMKGLMGNIGEHGLKGDKGEQGLPGVPGDIGFQGDKGSQGISGLPGARGKPGPTGKVGDKGPVGFPGPPGPEGFPGDIGPPGQNGPEGAKGKPGARGLPGPRGQLGQEGDEGPTGPPGSPGLEGQPGRKGFPGRPGPDGPKGEPGNSGRPGRMGEQGLMGFIGLVGEPGIVGEKGSRGAMGPPGAPGAKGSMGHPGSAGGVGDPGDPGPPGPPGPRGPLGMRGTKGRRGPRGPDGPAGEPGSKGVKGPPGTQGKQGLDGQQGQEGERGELGPRGFPGIPGPSGPPGAKGVPGEAGPQGPQGLIGPLGEIGPKGPPGALGEPGLPGEAGMKGDFGPLGIPGEQGLVGQRGETGLEGDSGPAGPDGLKGDKGEPGPDGERGEKGKEGLKGKEGPPGYPGITGVRGPEGKPGKQGEKGRTGPKGAKGYQGHLGEMGVPGEAGPAGNQGPKGSRGTLGPTGAPGRMGAQGEPGLPGYDGHKGLSGPIGPPGPKGEKGEQGDDGKIEGPPGPPGDRGPVGDRGDRGEPGDPGYPGQEGFEGSRGKAGQQGLPGHPGPRGRPGPKGSKGEEGQKGKQGKRGEAGSRGMQGLVGQPGPRGVVGRQGQEGPPGQDGIPGKDGRAGHQGEQGDDGEVGPIGPAGRRGNPGVAGLPGAQGPPGFKGESGLPGQLGPTGKRGTEGGTGFPGSQGEPGSKGQPGDFGEMGFPGIAGLFGPKGPPGDIGFKGIQGPRGPPGLMGKEGVIGPLGILGPSGIPGPKGNKGSRGDLGLQGPRGPPGPRGRPGPPGPPGIPISFQQDDLGAAFQTWMNSNSALRTEGYSHSDLLVLDQGGEIFKTLHYLSSLIQSIKKPLGTKETPARICRDLMNCEQKMADGTYWIDPNLGCASDTIEVTCNFTKGGQTCLRPITASKVDFAIGRVQMNFLHLLSSEVIQHITIHCLNMSVWQEGTGKVSPKQAVRFRAWNGQIFEAGGQFEPEVSSDDCKVQDGRWHQTLFIFRTQDPHQLPIVSVYNLPPATPGRQYRLEVGPACFL

Target ORF information:

RefSeq Version	XM_023494250.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii collagen type XXVII alpha 1 chain (COL27A1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023494250.1

ORF Insert Sequence:

ATGGAGCCGG AATCCGACCG GGTCTCCAGA GTCAGAGCAG CTGCGTCCAG GGGGGGATTC 
CTCCTAGCCT GGATTGCCTT CTGCTCTACC TGTGGCCTGG CCATCACCCA AGGAGATCCT 
GAAGATGTGG ATGTCCTGCA ACGGCTGGGC CTCGGGGGGA AGAAGTTGCC CGGCGGCCGG 
GCCCCAGGGC TGGCCCCCAT CCCCCCAGGA GTCATCCCGT TCCAGTCGGG GTTCATCTTC 
ACCCAGCGGG CCCGGATTGA AGCCCTTACA GGCGCCATCA TTCCCACCAC CTTCGGCACT 
GACCTGACCC TGGTGCTGAG CCTGTGCTCC CACCGAGTCA ACAACGCCTT CCTCTTTGCT 
GTCCGGAGCC GCAGGAAGAA GCTGCAGCTG GGGCTCCAGT TCATCCCTGG CAAGATCGTG 
CTGTTCCTGG GCCAGCGGCG CTCCCTGGCC TTCAGCTACG ACGTGCATGA CGGGCGATGG 
CACCACTTGG CCATCAGTCT CCGAGGCCAG GGGGCCACCC TCCTCACCTC CTGCGGGAAG 
CACCGCGTGC CCATGGATCT GCATTTTAAG AAAGAGGACT CTCTTGATCC CGAGGGGTCT 
TTCCTCTTTG GGAAGATGAA TCAGCACTCA GTCCAATTTG AGGGCGCCGT ATGCCAGTTC 
AATATTTATC CTTCTGCCAA GGCAGCTCAC AATTATTGTA AATATCTGAG GAAGCAGTGC 
AGACAGGCCG ACACCTACCG GCCACTGCTG CCCCCTCTTC TTCCCCTCCT TCCCCAGGAC 
TCCGAGGCCC ATACCCATCT TGGCCTCCAG CCCAGCACCT CTCTGCAGAG CCTGAGGAAT 
CTGACCACTG CGATGCCTTC GCTGGCACCC CTTAGAATGG CCAAGACCAC CCCGGCCTTG 
CCCAGAGCCC CCGGCCAAGG TGCCAGGGCT ACATGGGTGC CCTCAGCACC GGCCGGGCTT 
GGGGCACCCC CCCACCCGCC TGTGAGGACT AGTGCCGCGG ATCCCCGGGC GTCCCGATCC 
CCCACAGTGG GTACTTTGGT CTGGACCCTA CCAGCAACTG CAAAGCCATT GCCTTTCTCT 
TCAGGCAAAG CTTCCTCACC TGGGCTCAGG CCTGCACTGA TCACCCCTCA GAGCAACTTG 
ACCAAATCTC CCGTTCGCGC CATGAAGCCC ATGCCCCCAT CCAAGGCCAG TGTGGTTAAA 
ACTCCCCTTG TTCCCAGTAA GCCAGTAACT CCTTCAGTCA CTGTGGTCCC AGCTAAGAGG 
CCCCATCCCC TGGTCAAGCC GGTGCTGACC TCCCCTGCTA AGAAGCCAGT GCCCTCTGGC 
TCTCAGCCTG GCTCTGCCAA AGCCCCCCTT CCTCCCACAG CGAAACCAGC CCGTCAGAAC 
CCTGGAACAG CCAAAGCTCC TCCTTCCACT GCCAAGCCAG TGCTTCCCGC CGGGCGGTCC 
TCCCTTCCCG TTACCCCCAT GACAGCGTCC CAGGCTGCTC GGGCCAACGT GGCCAAAAAC 
CTCCCCTTTA CCAGCAAGCC GGCCAAGCTG ACCCACCCTT CCATTAAGTC ATCCTTGCCA 
GCCTCAGGTC CGAGCCTAAC CCAGACCCCT CGGCCCCCAG CAAAGCCGGT GCTTCCTACC 
GCGGGGATCA AATTGTCCAG AACAATCCCT CCCACCTTCT CCCCAGGCTC TCCTCCTGGG 
CTCAACTCCA CTGGTGACAG AAAAGTGGTT GAGCCCGCTG TGAAGAAGGA ATTGACTCCA 
AAGAGCACCC CCCAGCCACC CAGCCATCCC AGCCCTAGGA GGACTGGAGC AGTCCCTAGT 
ACCGTCTCCT TCCCTTCAGG CCTGAAGAAC CTGACCACGT GGCCCAGCCC CAAACAGGCT 
CAGCTCAGCC GTACAGCTGC CCCGGTTCTC CCACAGCACC CAGCCTCCAG CTCCCGTTTT 
GTGGGCGCCG TCACTCTCAC TCACACAGAT GGATACCTGT TCTTTGATCC AATTGGGCCC 
ACCTTGTTCC CACTGCTGTT GGGCCCTCCA GGGCAGAAGG GAGACCCTGG CCTCCCGGGC 
CCCCCTGGCT TGCCTGGGTT GCCTGGAATA TCAGGCAGAA GAGGACCCCG GGGTCCCCCT 
GGGCCTCATG GAAACCCTGG GCCTCCTGGC TCTCCTGGTG CAAAGGGACA AAAAGGAGAT 
CCTGGGCTTT CCCCTGGAAA GGCCTATGAT GGTGACAAGG GAGACGTAGG CTTACCAGGA 
CTGACTGGGA TTCCGGGACC AACTGGCCGG AAGGGGCTGA AAGGCTATCC TGGACCACCT 
GGGCATCCTG GAGACCAGGG AGAGCCAGGA CCAGAAGGGA GTCTGGGAGC CAAAGGTTAT 
CCTGGCAGGC AGGGATTACC TGGGCCAATA GGAGAACCTG GACCCAAAGG CAGTCGGGGG 
TATATTGGTC TCCCCGGGCT CTTTGGCCTG CCAGGACTTG ATGGAGAAAG GGGGCCTCCT 
GGAGCTCATG GCAAGAGGGG CAAGATGGGT AGGCCGGGAT TTCCTGGTGA CTTTGGGGAA 
AGAGGGCCCC CCGGATTGGA TGGCAACCCC GGAGAGCTGG GTGCCCCAGG CCCACCTGGA 
GTTCATGGAC TTATCGGTGA CATGGGAGCT ATTGGTCCAA TTGGCTACCC AGGGCCCAAG 
GGAATGAAGG GGCTTATGGG AAACATCGGA GAACACGGAC TGAAAGGTGA TAAGGGCGAG 
CAAGGGCTGC CTGGTGTGCC TGGAGATATC GGATTCCAAG GAGATAAGGG GAGTCAGGGA 
ATATCAGGGT TACCGGGTGC TCGAGGAAAG CCAGGTCCTA CAGGCAAGGT TGGTGACAAA 
GGACCAGTTG GGTTTCCTGG GCCTCCAGGT CCTGAGGGAT TCCCAGGGGA CATAGGACCA 
CCAGGCCAGA ATGGTCCAGA AGGTGCGAAG GGCAAGCCAG GAGCCCGAGG CTTACCAGGA 
CCGAGAGGAC AGCTTGGGCA GGAGGGAGAT GAGGGACCAA CGGGACCACC AGGATCCCCT 
GGCTTAGAGG GTCAACCTGG CCGGAAAGGA TTTCCAGGAA GGCCCGGCCC TGATGGCCCC 
AAGGGTGAGC CAGGAAACTC GGGTCGGCCT GGAAGAATGG GTGAGCAGGG CCTGATGGGA 
TTTATCGGTC TGGTCGGGGA GCCAGGAATC GTTGGAGAGA AGGGTAGCCG AGGTGCTATG 
GGACCACCAG GTGCTCCTGG AGCCAAGGGG TCGATGGGTC ATCCTGGTTC AGCAGGTGGA 
GTTGGAGATC CTGGAGACCC CGGGCCCCCA GGACCTCCTG GACCTCGTGG GCCACTGGGG 
ATGCGAGGAA CAAAAGGACG TCGGGGGCCC CGAGGACCAG ATGGACCAGC AGGGGAGCCA 
GGGTCAAAAG GTGTCAAGGG CCCTCCAGGA ACACAGGGCA AACAGGGCCT GGATGGACAG 
CAGGGCCAGG AAGGCGAGAG AGGGGAGCTG GGGCCTCGTG GCTTCCCGGG AATCCCGGGT 
CCTTCCGGCC CCCCGGGGGC TAAGGGCGTT CCAGGAGAAG CAGGCCCTCA AGGACCACAG 
GGACTGATTG GCCCATTGGG AGAGATTGGA CCTAAGGGAC CCCCTGGTGC TTTGGGAGAG 
CCTGGCCTTC CAGGTGAAGC TGGGATGAAG GGCGATTTTG GACCTTTGGG CATCCCTGGA 
GAGCAAGGTC TGGTTGGCCA GCGGGGAGAG ACAGGGCTGG AAGGAGATAG TGGCCCAGCT 
GGACCTGATG GTTTGAAGGG AGACAAGGGT GAGCCAGGCC CAGATGGCGA GCGAGGAGAG 
AAAGGCAAAG AAGGTCTGAA GGGCAAAGAA GGTCCCCCTG GCTATCCTGG GATCACAGGT 
GTCCGGGGTC CAGAAGGAAA ACCAGGCAAA CAAGGCGAGA AGGGACGAAC CGGACCTAAG 
GGTGCCAAGG GCTACCAAGG GCATCTTGGT GAGATGGGTG TTCCAGGAGA GGCCGGGCCA 
GCTGGCAACC AAGGCCCAAA AGGATCCCGA GGGACCCTGG GACCAACGGG TGCTCCAGGA 
AGAATGGGAG CTCAAGGAGA ACCTGGATTG CCTGGTTATG ATGGACATAA AGGACTATCA 
GGACCCATTG GACCTCCCGG GCCAAAAGGC GAGAAGGGAG AACAGGGTGA TGATGGGAAG 
ATTGAGGGCC CTCCAGGGCC TCCTGGAGAT CGGGGTCCAG TGGGTGATCG TGGAGACCGA 
GGGGAGCCTG GAGATCCTGG ATACCCTGGC CAGGAGGGTT TTGAAGGAAG CAGAGGAAAA 
GCTGGTCAGC AAGGCTTACC GGGACATCCT GGACCCCGGG GACGACCAGG ACCCAAAGGA 
TCAAAGGGTG AAGAGGGTCA GAAAGGGAAA CAAGGAAAGC GTGGAGAAGC TGGCAGTCGA 
GGAATGCAGG GCCTGGTGGG GCAGCCAGGG CCTCGAGGTG TGGTAGGAAG ACAAGGCCAA 
GAAGGACCCC CAGGTCAGGA TGGAATTCCT GGGAAGGATG GTCGAGCTGG ACATCAGGGG 
GAACAAGGAG ATGATGGAGA AGTTGGACCC ATTGGCCCTG CAGGAAGGAG AGGAAATCCA 
GGTGTGGCTG GTTTGCCTGG AGCTCAGGGA CCCCCAGGAT TCAAGGGTGA AAGTGGGTTG 
CCTGGACAAC TGGGCCCTAC TGGCAAGCGA GGGACAGAGG GAGGAACAGG GTTCCCTGGG 
AGCCAAGGGG AGCCAGGATC CAAAGGCCAG CCGGGTGACT TTGGAGAAAT GGGCTTTCCT 
GGGATTGCTG GCCTCTTTGG ACCCAAGGGC CCTCCAGGAG ATATTGGTTT CAAAGGAATC 
CAGGGGCCCC GGGGACCACC AGGTTTAATG GGAAAAGAAG GTGTCATTGG CCCCTTGGGG 
ATCCTAGGAC CGTCTGGAAT TCCGGGTCCA AAAGGGAACA AAGGAAGCCG AGGAGATTTG 
GGATTGCAAG GCCCAAGGGG TCCTCCTGGC CCCAGAGGAC GTCCTGGCCC ACCGGGCCCT 
CCTGGGATCC CCATCTCATT TCAGCAAGAT GACCTCGGAG CAGCTTTTCA GACGTGGATG 
AACTCCAACA GTGCACTTAG GACAGAGGGC TACTCCCATT CAGACCTCCT AGTGTTGGAC 
CAGGGTGGGG AGATCTTTAA GACCTTACAC TACCTCAGCA GCCTCATCCA GAGCATCAAG 
AAGCCTCTAG GAACCAAGGA GACCCCAGCC CGTATCTGTC GGGACCTTAT GAACTGTGAG 
CAGAAGATGG CCGATGGTAC ATATTGGATC GACCCAAATC TTGGCTGTGC TTCAGACACC 
ATCGAAGTCA CCTGCAATTT TACTAAAGGT GGACAGACAT GTCTCAGGCC CATCACTGCT 
TCAAAGGTGG ACTTTGCCAT TGGCCGGGTC CAGATGAATT TTCTTCACCT GCTGAGCTCG 
GAGGTGATCC AGCACATCAC TATCCACTGC CTCAACATGT CTGTATGGCA GGAGGGGACA 
GGCAAGGTGT CCCCCAAACA AGCCGTGCGC TTCCGAGCCT GGAATGGGCA GATCTTTGAA 
GCGGGCGGCC AGTTTGAGCC AGAGGTGTCC TCAGATGATT GCAAGGTTCA GGATGGCCGC 
TGGCACCAGA CCCTGTTCAT TTTCCGGACC CAAGACCCTC ACCAGCTGCC CATCGTCAGC 
GTCTACAACC TCCCTCCAGC CACCCCTGGG AGGCAATACC GCCTGGAAGT TGGACCTGCG 
TGCTTCCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL27A1 ( XM_023494250.2 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_023350018.1 Sarcophilus harrisii collagen type XXVII alpha 1 chain (COL27A1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSa150306
Clone ID Related Accession (Same CDS sequence)	XM_023494250.1 , XM_023494250.2
Accession Version	XM_023494250.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5712bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	collagen alpha-1(XXVII) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045427.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 31, 2019 this sequence version replaced XM_023494250.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCGG AATCCGACCG GGTCTCCAGA GTCAGAGCAG CTGCGTCCAG GGGGGGATTC 
CTCCTAGCCT GGATTGCCTT CTGCTCTACC TGTGGCCTGG CCATCACCCA AGGAGATCCT 
GAAGATGTGG ATGTCCTGCA ACGGCTGGGC CTCGGGGGGA AGAAGTTGCC CGGCGGCCGG 
GCCCCAGGGC TGGCCCCCAT CCCCCCAGGA GTCATCCCGT TCCAGTCGGG GTTCATCTTC 
ACCCAGCGGG CCCGGATTGA AGCCCTTACA GGCGCCATCA TTCCCACCAC CTTCGGCACT 
GACCTGACCC TGGTGCTGAG CCTGTGCTCC CACCGAGTCA ACAACGCCTT CCTCTTTGCT 
GTCCGGAGCC GCAGGAAGAA GCTGCAGCTG GGGCTCCAGT TCATCCCTGG CAAGATCGTG 
CTGTTCCTGG GCCAGCGGCG CTCCCTGGCC TTCAGCTACG ACGTGCATGA CGGGCGATGG 
CACCACTTGG CCATCAGTCT CCGAGGCCAG GGGGCCACCC TCCTCACCTC CTGCGGGAAG 
CACCGCGTGC CCATGGATCT GCATTTTAAG AAAGAGGACT CTCTTGATCC CGAGGGGTCT 
TTCCTCTTTG GGAAGATGAA TCAGCACTCA GTCCAATTTG AGGGCGCCGT ATGCCAGTTC 
AATATTTATC CTTCTGCCAA GGCAGCTCAC AATTATTGTA AATATCTGAG GAAGCAGTGC 
AGACAGGCCG ACACCTACCG GCCACTGCTG CCCCCTCTTC TTCCCCTCCT TCCCCAGGAC 
TCCGAGGCCC ATACCCATCT TGGCCTCCAG CCCAGCACCT CTCTGCAGAG CCTGAGGAAT 
CTGACCACTG CGATGCCTTC GCTGGCACCC CTTAGAATGG CCAAGACCAC CCCGGCCTTG 
CCCAGAGCCC CCGGCCAAGG TGCCAGGGCT ACATGGGTGC CCTCAGCACC GGCCGGGCTT 
GGGGCACCCC CCCACCCGCC TGTGAGGACT AGTGCCGCGG ATCCCCGGGC GTCCCGATCC 
CCCACAGTGG GTACTTTGGT CTGGACCCTA CCAGCAACTG CAAAGCCATT GCCTTTCTCT 
TCAGGCAAAG CTTCCTCACC TGGGCTCAGG CCTGCACTGA TCACCCCTCA GAGCAACTTG 
ACCAAATCTC CCGTTCGCGC CATGAAGCCC ATGCCCCCAT CCAAGGCCAG TGTGGTTAAA 
ACTCCCCTTG TTCCCAGTAA GCCAGTAACT CCTTCAGTCA CTGTGGTCCC AGCTAAGAGG 
CCCCATCCCC TGGTCAAGCC GGTGCTGACC TCCCCTGCTA AGAAGCCAGT GCCCTCTGGC 
TCTCAGCCTG GCTCTGCCAA AGCCCCCCTT CCTCCCACAG CGAAACCAGC CCGTCAGAAC 
CCTGGAACAG CCAAAGCTCC TCCTTCCACT GCCAAGCCAG TGCTTCCCGC CGGGCGGTCC 
TCCCTTCCCG TTACCCCCAT GACAGCGTCC CAGGCTGCTC GGGCCAACGT GGCCAAAAAC 
CTCCCCTTTA CCAGCAAGCC GGCCAAGCTG ACCCACCCTT CCATTAAGTC ATCCTTGCCA 
GCCTCAGGTC CGAGCCTAAC CCAGACCCCT CGGCCCCCAG CAAAGCCGGT GCTTCCTACC 
GCGGGGATCA AATTGTCCAG AACAATCCCT CCCACCTTCT CCCCAGGCTC TCCTCCTGGG 
CTCAACTCCA CTGGTGACAG AAAAGTGGTT GAGCCCGCTG TGAAGAAGGA ATTGACTCCA 
AAGAGCACCC CCCAGCCACC CAGCCATCCC AGCCCTAGGA GGACTGGAGC AGTCCCTAGT 
ACCGTCTCCT TCCCTTCAGG CCTGAAGAAC CTGACCACGT GGCCCAGCCC CAAACAGGCT 
CAGCTCAGCC GTACAGCTGC CCCGGTTCTC CCACAGCACC CAGCCTCCAG CTCCCGTTTT 
GTGGGCGCCG TCACTCTCAC TCACACAGAT GGATACCTGT TCTTTGATCC AATTGGGCCC 
ACCTTGTTCC CACTGCTGTT GGGCCCTCCA GGGCAGAAGG GAGACCCTGG CCTCCCGGGC 
CCCCCTGGCT TGCCTGGGTT GCCTGGAATA TCAGGCAGAA GAGGACCCCG GGGTCCCCCT 
GGGCCTCATG GAAACCCTGG GCCTCCTGGC TCTCCTGGTG CAAAGGGACA AAAAGGAGAT 
CCTGGGCTTT CCCCTGGAAA GGCCTATGAT GGTGACAAGG GAGACGTAGG CTTACCAGGA 
CTGACTGGGA TTCCGGGACC AACTGGCCGG AAGGGGCTGA AAGGCTATCC TGGACCACCT 
GGGCATCCTG GAGACCAGGG AGAGCCAGGA CCAGAAGGGA GTCTGGGAGC CAAAGGTTAT 
CCTGGCAGGC AGGGATTACC TGGGCCAATA GGAGAACCTG GACCCAAAGG CAGTCGGGGG 
TATATTGGTC TCCCCGGGCT CTTTGGCCTG CCAGGACTTG ATGGAGAAAG GGGGCCTCCT 
GGAGCTCATG GCAAGAGGGG CAAGATGGGT AGGCCGGGAT TTCCTGGTGA CTTTGGGGAA 
AGAGGGCCCC CCGGATTGGA TGGCAACCCC GGAGAGCTGG GTGCCCCAGG CCCACCTGGA 
GTTCATGGAC TTATCGGTGA CATGGGAGCT ATTGGTCCAA TTGGCTACCC AGGGCCCAAG 
GGAATGAAGG GGCTTATGGG AAACATCGGA GAACACGGAC TGAAAGGTGA TAAGGGCGAG 
CAAGGGCTGC CTGGTGTGCC TGGAGATATC GGATTCCAAG GAGATAAGGG GAGTCAGGGA 
ATATCAGGGT TACCGGGTGC TCGAGGAAAG CCAGGTCCTA CAGGCAAGGT TGGTGACAAA 
GGACCAGTTG GGTTTCCTGG GCCTCCAGGT CCTGAGGGAT TCCCAGGGGA CATAGGACCA 
CCAGGCCAGA ATGGTCCAGA AGGTGCGAAG GGCAAGCCAG GAGCCCGAGG CTTACCAGGA 
CCGAGAGGAC AGCTTGGGCA GGAGGGAGAT GAGGGACCAA CGGGACCACC AGGATCCCCT 
GGCTTAGAGG GTCAACCTGG CCGGAAAGGA TTTCCAGGAA GGCCCGGCCC TGATGGCCCC 
AAGGGTGAGC CAGGAAACTC GGGTCGGCCT GGAAGAATGG GTGAGCAGGG CCTGATGGGA 
TTTATCGGTC TGGTCGGGGA GCCAGGAATC GTTGGAGAGA AGGGTAGCCG AGGTGCTATG 
GGACCACCAG GTGCTCCTGG AGCCAAGGGG TCGATGGGTC ATCCTGGTTC AGCAGGTGGA 
GTTGGAGATC CTGGAGACCC CGGGCCCCCA GGACCTCCTG GACCTCGTGG GCCACTGGGG 
ATGCGAGGAA CAAAAGGACG TCGGGGGCCC CGAGGACCAG ATGGACCAGC AGGGGAGCCA 
GGGTCAAAAG GTGTCAAGGG CCCTCCAGGA ACACAGGGCA AACAGGGCCT GGATGGACAG 
CAGGGCCAGG AAGGCGAGAG AGGGGAGCTG GGGCCTCGTG GCTTCCCGGG AATCCCGGGT 
CCTTCCGGCC CCCCGGGGGC TAAGGGCGTT CCAGGAGAAG CAGGCCCTCA AGGACCACAG 
GGACTGATTG GCCCATTGGG AGAGATTGGA CCTAAGGGAC CCCCTGGTGC TTTGGGAGAG 
CCTGGCCTTC CAGGTGAAGC TGGGATGAAG GGCGATTTTG GACCTTTGGG CATCCCTGGA 
GAGCAAGGTC TGGTTGGCCA GCGGGGAGAG ACAGGGCTGG AAGGAGATAG TGGCCCAGCT 
GGACCTGATG GTTTGAAGGG AGACAAGGGT GAGCCAGGCC CAGATGGCGA GCGAGGAGAG 
AAAGGCAAAG AAGGTCTGAA GGGCAAAGAA GGTCCCCCTG GCTATCCTGG GATCACAGGT 
GTCCGGGGTC CAGAAGGAAA ACCAGGCAAA CAAGGCGAGA AGGGACGAAC CGGACCTAAG 
GGTGCCAAGG GCTACCAAGG GCATCTTGGT GAGATGGGTG TTCCAGGAGA GGCCGGGCCA 
GCTGGCAACC AAGGCCCAAA AGGATCCCGA GGGACCCTGG GACCAACGGG TGCTCCAGGA 
AGAATGGGAG CTCAAGGAGA ACCTGGATTG CCTGGTTATG ATGGACATAA AGGACTATCA 
GGACCCATTG GACCTCCCGG GCCAAAAGGC GAGAAGGGAG AACAGGGTGA TGATGGGAAG 
ATTGAGGGCC CTCCAGGGCC TCCTGGAGAT CGGGGTCCAG TGGGTGATCG TGGAGACCGA 
GGGGAGCCTG GAGATCCTGG ATACCCTGGC CAGGAGGGTT TTGAAGGAAG CAGAGGAAAA 
GCTGGTCAGC AAGGCTTACC GGGACATCCT GGACCCCGGG GACGACCAGG ACCCAAAGGA 
TCAAAGGGTG AAGAGGGTCA GAAAGGGAAA CAAGGAAAGC GTGGAGAAGC TGGCAGTCGA 
GGAATGCAGG GCCTGGTGGG GCAGCCAGGG CCTCGAGGTG TGGTAGGAAG ACAAGGCCAA 
GAAGGACCCC CAGGTCAGGA TGGAATTCCT GGGAAGGATG GTCGAGCTGG ACATCAGGGG 
GAACAAGGAG ATGATGGAGA AGTTGGACCC ATTGGCCCTG CAGGAAGGAG AGGAAATCCA 
GGTGTGGCTG GTTTGCCTGG AGCTCAGGGA CCCCCAGGAT TCAAGGGTGA AAGTGGGTTG 
CCTGGACAAC TGGGCCCTAC TGGCAAGCGA GGGACAGAGG GAGGAACAGG GTTCCCTGGG 
AGCCAAGGGG AGCCAGGATC CAAAGGCCAG CCGGGTGACT TTGGAGAAAT GGGCTTTCCT 
GGGATTGCTG GCCTCTTTGG ACCCAAGGGC CCTCCAGGAG ATATTGGTTT CAAAGGAATC 
CAGGGGCCCC GGGGACCACC AGGTTTAATG GGAAAAGAAG GTGTCATTGG CCCCTTGGGG 
ATCCTAGGAC CGTCTGGAAT TCCGGGTCCA AAAGGGAACA AAGGAAGCCG AGGAGATTTG 
GGATTGCAAG GCCCAAGGGG TCCTCCTGGC CCCAGAGGAC GTCCTGGCCC ACCGGGCCCT 
CCTGGGATCC CCATCTCATT TCAGCAAGAT GACCTCGGAG CAGCTTTTCA GACGTGGATG 
AACTCCAACA GTGCACTTAG GACAGAGGGC TACTCCCATT CAGACCTCCT AGTGTTGGAC 
CAGGGTGGGG AGATCTTTAA GACCTTACAC TACCTCAGCA GCCTCATCCA GAGCATCAAG 
AAGCCTCTAG GAACCAAGGA GACCCCAGCC CGTATCTGTC GGGACCTTAT GAACTGTGAG 
CAGAAGATGG CCGATGGTAC ATATTGGATC GACCCAAATC TTGGCTGTGC TTCAGACACC 
ATCGAAGTCA CCTGCAATTT TACTAAAGGT GGACAGACAT GTCTCAGGCC CATCACTGCT 
TCAAAGGTGG ACTTTGCCAT TGGCCGGGTC CAGATGAATT TTCTTCACCT GCTGAGCTCG 
GAGGTGATCC AGCACATCAC TATCCACTGC CTCAACATGT CTGTATGGCA GGAGGGGACA 
GGCAAGGTGT CCCCCAAACA AGCCGTGCGC TTCCGAGCCT GGAATGGGCA GATCTTTGAA 
GCGGGCGGCC AGTTTGAGCC AGAGGTGTCC TCAGATGATT GCAAGGTTCA GGATGGCCGC 
TGGCACCAGA CCCTGTTCAT TTTCCGGACC CAAGACCCTC ACCAGCTGCC CATCGTCAGC 
GTCTACAACC TCCCTCCAGC CACCCCTGGG AGGCAATACC GCCTGGAAGT TGGACCTGCG 
TGCTTCCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023350018.1
CDS	1237..6948
Translation	MEPESDRVSRVRAAASRGGFLLAWIAFCSTCGLAITQGDPEDVDVLQRLGLGGKKLPGGRAPGLAPIPPGVIPFQSGFIFTQRARIEALTGAIIPTTFGTDLTLVLSLCSHRVNNAFLFAVRSRRKKLQLGLQFIPGKIVLFLGQRRSLAFSYDVHDGRWHHLAISLRGQGATLLTSCGKHRVPMDLHFKKEDSLDPEGSFLFGKMNQHSVQFEGAVCQFNIYPSAKAAHNYCKYLRKQCRQADTYRPLLPPLLPLLPQDSEAHTHLGLQPSTSLQSLRNLTTAMPSLAPLRMAKTTPALPRAPGQGARATWVPSAPAGLGAPPHPPVRTSAADPRASRSPTVGTLVWTLPATAKPLPFSSGKASSPGLRPALITPQSNLTKSPVRAMKPMPPSKASVVKTPLVPSKPVTPSVTVVPAKRPHPLVKPVLTSPAKKPVPSGSQPGSAKAPLPPTAKPARQNPGTAKAPPSTAKPVLPAGRSSLPVTPMTASQAARANVAKNLPFTSKPAKLTHPSIKSSLPASGPSLTQTPRPPAKPVLPTAGIKLSRTIPPTFSPGSPPGLNSTGDRKVVEPAVKKELTPKSTPQPPSHPSPRRTGAVPSTVSFPSGLKNLTTWPSPKQAQLSRTAAPVLPQHPASSSRFVGAVTLTHTDGYLFFDPIGPTLFPLLLGPPGQKGDPGLPGPPGLPGLPGISGRRGPRGPPGPHGNPGPPGSPGAKGQKGDPGLSPGKAYDGDKGDVGLPGLTGIPGPTGRKGLKGYPGPPGHPGDQGEPGPEGSLGAKGYPGRQGLPGPIGEPGPKGSRGYIGLPGLFGLPGLDGERGPPGAHGKRGKMGRPGFPGDFGERGPPGLDGNPGELGAPGPPGVHGLIGDMGAIGPIGYPGPKGMKGLMGNIGEHGLKGDKGEQGLPGVPGDIGFQGDKGSQGISGLPGARGKPGPTGKVGDKGPVGFPGPPGPEGFPGDIGPPGQNGPEGAKGKPGARGLPGPRGQLGQEGDEGPTGPPGSPGLEGQPGRKGFPGRPGPDGPKGEPGNSGRPGRMGEQGLMGFIGLVGEPGIVGEKGSRGAMGPPGAPGAKGSMGHPGSAGGVGDPGDPGPPGPPGPRGPLGMRGTKGRRGPRGPDGPAGEPGSKGVKGPPGTQGKQGLDGQQGQEGERGELGPRGFPGIPGPSGPPGAKGVPGEAGPQGPQGLIGPLGEIGPKGPPGALGEPGLPGEAGMKGDFGPLGIPGEQGLVGQRGETGLEGDSGPAGPDGLKGDKGEPGPDGERGEKGKEGLKGKEGPPGYPGITGVRGPEGKPGKQGEKGRTGPKGAKGYQGHLGEMGVPGEAGPAGNQGPKGSRGTLGPTGAPGRMGAQGEPGLPGYDGHKGLSGPIGPPGPKGEKGEQGDDGKIEGPPGPPGDRGPVGDRGDRGEPGDPGYPGQEGFEGSRGKAGQQGLPGHPGPRGRPGPKGSKGEEGQKGKQGKRGEAGSRGMQGLVGQPGPRGVVGRQGQEGPPGQDGIPGKDGRAGHQGEQGDDGEVGPIGPAGRRGNPGVAGLPGAQGPPGFKGESGLPGQLGPTGKRGTEGGTGFPGSQGEPGSKGQPGDFGEMGFPGIAGLFGPKGPPGDIGFKGIQGPRGPPGLMGKEGVIGPLGILGPSGIPGPKGNKGSRGDLGLQGPRGPPGPRGRPGPPGPPGIPISFQQDDLGAAFQTWMNSNSALRTEGYSHSDLLVLDQGGEIFKTLHYLSSLIQSIKKPLGTKETPARICRDLMNCEQKMADGTYWIDPNLGCASDTIEVTCNFTKGGQTCLRPITASKVDFAIGRVQMNFLHLLSSEVIQHITIHCLNMSVWQEGTGKVSPKQAVRFRAWNGQIFEAGGQFEPEVSSDDCKVQDGRWHQTLFIFRTQDPHQLPIVSVYNLPPATPGRQYRLEVGPACFL

Target ORF information:

RefSeq Version	XM_023494250.2
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii collagen type XXVII alpha 1 chain (COL27A1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023494250.2

ORF Insert Sequence:

ATGGAGCCGG AATCCGACCG GGTCTCCAGA GTCAGAGCAG CTGCGTCCAG GGGGGGATTC 
CTCCTAGCCT GGATTGCCTT CTGCTCTACC TGTGGCCTGG CCATCACCCA AGGAGATCCT 
GAAGATGTGG ATGTCCTGCA ACGGCTGGGC CTCGGGGGGA AGAAGTTGCC CGGCGGCCGG 
GCCCCAGGGC TGGCCCCCAT CCCCCCAGGA GTCATCCCGT TCCAGTCGGG GTTCATCTTC 
ACCCAGCGGG CCCGGATTGA AGCCCTTACA GGCGCCATCA TTCCCACCAC CTTCGGCACT 
GACCTGACCC TGGTGCTGAG CCTGTGCTCC CACCGAGTCA ACAACGCCTT CCTCTTTGCT 
GTCCGGAGCC GCAGGAAGAA GCTGCAGCTG GGGCTCCAGT TCATCCCTGG CAAGATCGTG 
CTGTTCCTGG GCCAGCGGCG CTCCCTGGCC TTCAGCTACG ACGTGCATGA CGGGCGATGG 
CACCACTTGG CCATCAGTCT CCGAGGCCAG GGGGCCACCC TCCTCACCTC CTGCGGGAAG 
CACCGCGTGC CCATGGATCT GCATTTTAAG AAAGAGGACT CTCTTGATCC CGAGGGGTCT 
TTCCTCTTTG GGAAGATGAA TCAGCACTCA GTCCAATTTG AGGGCGCCGT ATGCCAGTTC 
AATATTTATC CTTCTGCCAA GGCAGCTCAC AATTATTGTA AATATCTGAG GAAGCAGTGC 
AGACAGGCCG ACACCTACCG GCCACTGCTG CCCCCTCTTC TTCCCCTCCT TCCCCAGGAC 
TCCGAGGCCC ATACCCATCT TGGCCTCCAG CCCAGCACCT CTCTGCAGAG CCTGAGGAAT 
CTGACCACTG CGATGCCTTC GCTGGCACCC CTTAGAATGG CCAAGACCAC CCCGGCCTTG 
CCCAGAGCCC CCGGCCAAGG TGCCAGGGCT ACATGGGTGC CCTCAGCACC GGCCGGGCTT 
GGGGCACCCC CCCACCCGCC TGTGAGGACT AGTGCCGCGG ATCCCCGGGC GTCCCGATCC 
CCCACAGTGG GTACTTTGGT CTGGACCCTA CCAGCAACTG CAAAGCCATT GCCTTTCTCT 
TCAGGCAAAG CTTCCTCACC TGGGCTCAGG CCTGCACTGA TCACCCCTCA GAGCAACTTG 
ACCAAATCTC CCGTTCGCGC CATGAAGCCC ATGCCCCCAT CCAAGGCCAG TGTGGTTAAA 
ACTCCCCTTG TTCCCAGTAA GCCAGTAACT CCTTCAGTCA CTGTGGTCCC AGCTAAGAGG 
CCCCATCCCC TGGTCAAGCC GGTGCTGACC TCCCCTGCTA AGAAGCCAGT GCCCTCTGGC 
TCTCAGCCTG GCTCTGCCAA AGCCCCCCTT CCTCCCACAG CGAAACCAGC CCGTCAGAAC 
CCTGGAACAG CCAAAGCTCC TCCTTCCACT GCCAAGCCAG TGCTTCCCGC CGGGCGGTCC 
TCCCTTCCCG TTACCCCCAT GACAGCGTCC CAGGCTGCTC GGGCCAACGT GGCCAAAAAC 
CTCCCCTTTA CCAGCAAGCC GGCCAAGCTG ACCCACCCTT CCATTAAGTC ATCCTTGCCA 
GCCTCAGGTC CGAGCCTAAC CCAGACCCCT CGGCCCCCAG CAAAGCCGGT GCTTCCTACC 
GCGGGGATCA AATTGTCCAG AACAATCCCT CCCACCTTCT CCCCAGGCTC TCCTCCTGGG 
CTCAACTCCA CTGGTGACAG AAAAGTGGTT GAGCCCGCTG TGAAGAAGGA ATTGACTCCA 
AAGAGCACCC CCCAGCCACC CAGCCATCCC AGCCCTAGGA GGACTGGAGC AGTCCCTAGT 
ACCGTCTCCT TCCCTTCAGG CCTGAAGAAC CTGACCACGT GGCCCAGCCC CAAACAGGCT 
CAGCTCAGCC GTACAGCTGC CCCGGTTCTC CCACAGCACC CAGCCTCCAG CTCCCGTTTT 
GTGGGCGCCG TCACTCTCAC TCACACAGAT GGATACCTGT TCTTTGATCC AATTGGGCCC 
ACCTTGTTCC CACTGCTGTT GGGCCCTCCA GGGCAGAAGG GAGACCCTGG CCTCCCGGGC 
CCCCCTGGCT TGCCTGGGTT GCCTGGAATA TCAGGCAGAA GAGGACCCCG GGGTCCCCCT 
GGGCCTCATG GAAACCCTGG GCCTCCTGGC TCTCCTGGTG CAAAGGGACA AAAAGGAGAT 
CCTGGGCTTT CCCCTGGAAA GGCCTATGAT GGTGACAAGG GAGACGTAGG CTTACCAGGA 
CTGACTGGGA TTCCGGGACC AACTGGCCGG AAGGGGCTGA AAGGCTATCC TGGACCACCT 
GGGCATCCTG GAGACCAGGG AGAGCCAGGA CCAGAAGGGA GTCTGGGAGC CAAAGGTTAT 
CCTGGCAGGC AGGGATTACC TGGGCCAATA GGAGAACCTG GACCCAAAGG CAGTCGGGGG 
TATATTGGTC TCCCCGGGCT CTTTGGCCTG CCAGGACTTG ATGGAGAAAG GGGGCCTCCT 
GGAGCTCATG GCAAGAGGGG CAAGATGGGT AGGCCGGGAT TTCCTGGTGA CTTTGGGGAA 
AGAGGGCCCC CCGGATTGGA TGGCAACCCC GGAGAGCTGG GTGCCCCAGG CCCACCTGGA 
GTTCATGGAC TTATCGGTGA CATGGGAGCT ATTGGTCCAA TTGGCTACCC AGGGCCCAAG 
GGAATGAAGG GGCTTATGGG AAACATCGGA GAACACGGAC TGAAAGGTGA TAAGGGCGAG 
CAAGGGCTGC CTGGTGTGCC TGGAGATATC GGATTCCAAG GAGATAAGGG GAGTCAGGGA 
ATATCAGGGT TACCGGGTGC TCGAGGAAAG CCAGGTCCTA CAGGCAAGGT TGGTGACAAA 
GGACCAGTTG GGTTTCCTGG GCCTCCAGGT CCTGAGGGAT TCCCAGGGGA CATAGGACCA 
CCAGGCCAGA ATGGTCCAGA AGGTGCGAAG GGCAAGCCAG GAGCCCGAGG CTTACCAGGA 
CCGAGAGGAC AGCTTGGGCA GGAGGGAGAT GAGGGACCAA CGGGACCACC AGGATCCCCT 
GGCTTAGAGG GTCAACCTGG CCGGAAAGGA TTTCCAGGAA GGCCCGGCCC TGATGGCCCC 
AAGGGTGAGC CAGGAAACTC GGGTCGGCCT GGAAGAATGG GTGAGCAGGG CCTGATGGGA 
TTTATCGGTC TGGTCGGGGA GCCAGGAATC GTTGGAGAGA AGGGTAGCCG AGGTGCTATG 
GGACCACCAG GTGCTCCTGG AGCCAAGGGG TCGATGGGTC ATCCTGGTTC AGCAGGTGGA 
GTTGGAGATC CTGGAGACCC CGGGCCCCCA GGACCTCCTG GACCTCGTGG GCCACTGGGG 
ATGCGAGGAA CAAAAGGACG TCGGGGGCCC CGAGGACCAG ATGGACCAGC AGGGGAGCCA 
GGGTCAAAAG GTGTCAAGGG CCCTCCAGGA ACACAGGGCA AACAGGGCCT GGATGGACAG 
CAGGGCCAGG AAGGCGAGAG AGGGGAGCTG GGGCCTCGTG GCTTCCCGGG AATCCCGGGT 
CCTTCCGGCC CCCCGGGGGC TAAGGGCGTT CCAGGAGAAG CAGGCCCTCA AGGACCACAG 
GGACTGATTG GCCCATTGGG AGAGATTGGA CCTAAGGGAC CCCCTGGTGC TTTGGGAGAG 
CCTGGCCTTC CAGGTGAAGC TGGGATGAAG GGCGATTTTG GACCTTTGGG CATCCCTGGA 
GAGCAAGGTC TGGTTGGCCA GCGGGGAGAG ACAGGGCTGG AAGGAGATAG TGGCCCAGCT 
GGACCTGATG GTTTGAAGGG AGACAAGGGT GAGCCAGGCC CAGATGGCGA GCGAGGAGAG 
AAAGGCAAAG AAGGTCTGAA GGGCAAAGAA GGTCCCCCTG GCTATCCTGG GATCACAGGT 
GTCCGGGGTC CAGAAGGAAA ACCAGGCAAA CAAGGCGAGA AGGGACGAAC CGGACCTAAG 
GGTGCCAAGG GCTACCAAGG GCATCTTGGT GAGATGGGTG TTCCAGGAGA GGCCGGGCCA 
GCTGGCAACC AAGGCCCAAA AGGATCCCGA GGGACCCTGG GACCAACGGG TGCTCCAGGA 
AGAATGGGAG CTCAAGGAGA ACCTGGATTG CCTGGTTATG ATGGACATAA AGGACTATCA 
GGACCCATTG GACCTCCCGG GCCAAAAGGC GAGAAGGGAG AACAGGGTGA TGATGGGAAG 
ATTGAGGGCC CTCCAGGGCC TCCTGGAGAT CGGGGTCCAG TGGGTGATCG TGGAGACCGA 
GGGGAGCCTG GAGATCCTGG ATACCCTGGC CAGGAGGGTT TTGAAGGAAG CAGAGGAAAA 
GCTGGTCAGC AAGGCTTACC GGGACATCCT GGACCCCGGG GACGACCAGG ACCCAAAGGA 
TCAAAGGGTG AAGAGGGTCA GAAAGGGAAA CAAGGAAAGC GTGGAGAAGC TGGCAGTCGA 
GGAATGCAGG GCCTGGTGGG GCAGCCAGGG CCTCGAGGTG TGGTAGGAAG ACAAGGCCAA 
GAAGGACCCC CAGGTCAGGA TGGAATTCCT GGGAAGGATG GTCGAGCTGG ACATCAGGGG 
GAACAAGGAG ATGATGGAGA AGTTGGACCC ATTGGCCCTG CAGGAAGGAG AGGAAATCCA 
GGTGTGGCTG GTTTGCCTGG AGCTCAGGGA CCCCCAGGAT TCAAGGGTGA AAGTGGGTTG 
CCTGGACAAC TGGGCCCTAC TGGCAAGCGA GGGACAGAGG GAGGAACAGG GTTCCCTGGG 
AGCCAAGGGG AGCCAGGATC CAAAGGCCAG CCGGGTGACT TTGGAGAAAT GGGCTTTCCT 
GGGATTGCTG GCCTCTTTGG ACCCAAGGGC CCTCCAGGAG ATATTGGTTT CAAAGGAATC 
CAGGGGCCCC GGGGACCACC AGGTTTAATG GGAAAAGAAG GTGTCATTGG CCCCTTGGGG 
ATCCTAGGAC CGTCTGGAAT TCCGGGTCCA AAAGGGAACA AAGGAAGCCG AGGAGATTTG 
GGATTGCAAG GCCCAAGGGG TCCTCCTGGC CCCAGAGGAC GTCCTGGCCC ACCGGGCCCT 
CCTGGGATCC CCATCTCATT TCAGCAAGAT GACCTCGGAG CAGCTTTTCA GACGTGGATG 
AACTCCAACA GTGCACTTAG GACAGAGGGC TACTCCCATT CAGACCTCCT AGTGTTGGAC 
CAGGGTGGGG AGATCTTTAA GACCTTACAC TACCTCAGCA GCCTCATCCA GAGCATCAAG 
AAGCCTCTAG GAACCAAGGA GACCCCAGCC CGTATCTGTC GGGACCTTAT GAACTGTGAG 
CAGAAGATGG CCGATGGTAC ATATTGGATC GACCCAAATC TTGGCTGTGC TTCAGACACC 
ATCGAAGTCA CCTGCAATTT TACTAAAGGT GGACAGACAT GTCTCAGGCC CATCACTGCT 
TCAAAGGTGG ACTTTGCCAT TGGCCGGGTC CAGATGAATT TTCTTCACCT GCTGAGCTCG 
GAGGTGATCC AGCACATCAC TATCCACTGC CTCAACATGT CTGTATGGCA GGAGGGGACA 
GGCAAGGTGT CCCCCAAACA AGCCGTGCGC TTCCGAGCCT GGAATGGGCA GATCTTTGAA 
GCGGGCGGCC AGTTTGAGCC AGAGGTGTCC TCAGATGATT GCAAGGTTCA GGATGGCCGC 
TGGCACCAGA CCCTGTTCAT TTTCCGGACC CAAGACCCTC ACCAGCTGCC CATCGTCAGC 
GTCTACAACC TCCCTCCAGC CACCCCTGGG AGGCAATACC GCCTGGAAGT TGGACCTGCG 
TGCTTCCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL27A1 ( XM_023494251.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_023350019.1 Sarcophilus harrisii collagen type XXVII alpha 1 chain (COL27A1), transcript variant X2, mRNA.

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CloneID	OSa150307
Clone ID Related Accession (Same CDS sequence)	XM_023494251.1
Accession Version	XM_023494251.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5658bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515513600000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	collagen alpha-1(XXVII) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003816661.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCGG AATCCGACCG GGTCTCCAGA GTCAGAGCAG CTGCGTCCAG GGGGGGATTC 
CTCCTAGCCT GGATTGCCTT CTGCTCTACC TGTGGCCTGG CCATCACCCA AGGAGATCCT 
GAAGATGTGG ATGTCCTGCA ACGGCTGGGC CTCGGGGGGA AGAAGTTGCC CGGCGGCCGG 
GCCCCAGGGC TGGCCCCCAT CCCCCCAGGA GTCATCCCGT TCCAGTCGGG GTTCATCTTC 
ACCCAGCGGG CCCGGATTGA AGCCCTTACA GGCGCCATCA TTCCCACCAC CTTCGGCACT 
GACCTGACCC TGGTGCTGAG CCTGTGCTCC CACCGAGTCA ACAACGCCTT CCTCTTTGCT 
GTCCGGAGCC GCAGGAAGAA GCTGCAGCTG GGGCTCCAGT TCATCCCTGG CAAGATCGTG 
CTGTTCCTGG GCCAGCGGCG CTCCCTGGCC TTCAGCTACG ACGTGCATGA CGGGCGATGG 
CACCACTTGG CCATCAGTCT CCGAGGCCAG GGGGCCACCC TCCTCACCTC CTGCGGGAAG 
CACCGCGTGC CCATGGATCT GCATTTTAAG AAAGAGGACT CTCTTGATCC CGAGGGGTCT 
TTCCTCTTTG GGAAGATGAA TCAGCACTCA GTCCAATTTG AGGGCGCCGT ATGCCAGTTC 
AATATTTATC CTTCTGCCAA GGCAGCTCAC AATTATTGTA AATATCTGAG GAAGCAGTGC 
AGACAGGCCG ACACCTACCG GCCACTGCTG CCCCCTCTTC TTCCCCTCCT TCCCCAGGAC 
TCCGAGGCCC ATACCCATCT TGGCCTCCAG CCCAGCACCT CTCTGCAGAG CCTGAGGAAT 
CTGACCACTG CGATGCCTTC GCTGGCACCC CTTAGAATGG CCAAGACCAC CCCGGCCTTG 
CCCAGAGCCC CCGGCCAAGG TGCCAGGGCT ACATGGGTGC CCTCAGCACC GGCCGGGCTT 
GGGGCACCCC CCCACCCGCC TGTGAGGACT AGTGCCGCGG ATCCCCGGGC GTCCCGATCC 
CCCACAGTGG GTACTTTGGT CTGGACCCTA CCAGCAACTG CAAAGCCATT GCCTTTCTCT 
TCAGGCAAAG CTTCCTCACC TGGGCTCAGG CCTGCACTGA TCACCCCTCA GAGCAACTTG 
ACCAAATCTC CCGTTCGCGC CATGAAGCCC ATGCCCCCAT CCAAGGCCAG TGTGGTTAAA 
ACTCCCCTTG TTCCCAGTAA GCCAGTAACT CCTTCAGTCA CTGTGGTCCC AGCTAAGAGG 
CCCCATCCCC TGGTCAAGCC GGTGCTGACC TCCCCTGCTA AGAAGCCAGT GCCCTCTGGC 
TCTCAGCCTG GCTCTGCCAA AGCCCCCCTT CCTCCCACAG CGAAACCAGC CCGTCAGAAC 
CCTGGAACAG CCAAAGCTCC TCCTTCCACT GCCAAGCCAG TGCTTCCCGC CGGGCGGTCC 
TCCCTTCCCG TTACCCCCAT GACAGCGTCC CAGGCTGCTC GGGCCAACGT GGCCAAAAAC 
CTCCCCTTTA CCAGCAAGCC GGCCAAGCTG ACCCACCCTT CCATTAAGTC ATCCTTGCCA 
GCCTCAGGTC CGAGCCTAAC CCAGACCCCT CGGCCCCCAG CAAAGCCGGT GCTTCCTACC 
GCGGGGATCA AATTGTCCAG AACAATCCCT CCCACCTTCT CCCCAGGCTC TCCTCCTGGG 
CTCAACTCCA CTGGTGACAG AAAAGTGGTT GAGCCCGCTG TGAAGAAGGA ATTGACTCCA 
AAGAGCACCC CCCAGCCACC CAGCCATCCC AGCCCTAGGA GGACTGGAGC AGTCCCTAGT 
ACCGTCTCCT TCCCTTCAGG CCTGAAGAAC CTGACCACGT GGCCCAGCCC CAAACAGGCT 
CAGCTCAGCC GTACAGCTGC CCCGGTTCTC CCACAGCACC CAGCCTCCAG CTCCCGTTTT 
GTGGGCGCCG TCACTCTCAC TCACACAGAT GGATACCTGT TCTTTGATCC AATTGGGCCC 
ACCTTGTTCC CACTGCTGTT GGGCCCTCCA GGGCAGAAGG GAGACCCTGG CCTCCCGGGC 
CCCCCTGGCT TGCCTGGGTT GCCTGGAATA TCAGGCAGAA GAGGACCCCG GGGACAAAAA 
GGAGATCCTG GGCTTTCCCC TGGAAAGGCC TATGATGGTG ACAAGGGAGA CGTAGGCTTA 
CCAGGACTGA CTGGGATTCC GGGACCAACT GGCCGGAAGG GGCTGAAAGG CTATCCTGGA 
CCACCTGGGC ATCCTGGAGA CCAGGGAGAG CCAGGACCAG AAGGGAGTCT GGGAGCCAAA 
GGTTATCCTG GCAGGCAGGG ATTACCTGGG CCAATAGGAG AACCTGGACC CAAAGGCAGT 
CGGGGGTATA TTGGTCTCCC CGGGCTCTTT GGCCTGCCAG GACTTGATGG AGAAAGGGGG 
CCTCCTGGAG CTCATGGCAA GAGGGGCAAG ATGGGTAGGC CGGGATTTCC TGGTGACTTT 
GGGGAAAGAG GGCCCCCCGG ATTGGATGGC AACCCCGGAG AGCTGGGTGC CCCAGGCCCA 
CCTGGAGTTC ATGGACTTAT CGGTGACATG GGAGCTATTG GTCCAATTGG CTACCCAGGG 
CCCAAGGGAA TGAAGGGGCT TATGGGAAAC ATCGGAGAAC ACGGACTGAA AGGTGATAAG 
GGCGAGCAAG GGCTGCCTGG TGTGCCTGGA GATATCGGAT TCCAAGGAGA TAAGGGGAGT 
CAGGGAATAT CAGGGTTACC GGGTGCTCGA GGAAAGCCAG GTCCTACAGG CAAGGTTGGT 
GACAAAGGAC CAGTTGGGTT TCCTGGGCCT CCAGGTCCTG AGGGATTCCC AGGGGACATA 
GGACCACCAG GCCAGAATGG TCCAGAAGGT GCGAAGGGCA AGCCAGGAGC CCGAGGCTTA 
CCAGGACCGA GAGGACAGCT TGGGCAGGAG GGAGATGAGG GACCAACGGG ACCACCAGGA 
TCCCCTGGCT TAGAGGGTCA ACCTGGCCGG AAAGGATTTC CAGGAAGGCC CGGCCCTGAT 
GGCCCCAAGG GTGAGCCAGG AAACTCGGGT CGGCCTGGAA GAATGGGTGA GCAGGGCCTG 
ATGGGATTTA TCGGTCTGGT CGGGGAGCCA GGAATCGTTG GAGAGAAGGG TAGCCGAGGT 
GCTATGGGAC CACCAGGTGC TCCTGGAGCC AAGGGGTCGA TGGGTCATCC TGGTTCAGCA 
GGTGGAGTTG GAGATCCTGG AGACCCCGGG CCCCCAGGAC CTCCTGGACC TCGTGGGCCA 
CTGGGGATGC GAGGAACAAA AGGACGTCGG GGGCCCCGAG GACCAGATGG ACCAGCAGGG 
GAGCCAGGGT CAAAAGGTGT CAAGGGCCCT CCAGGAACAC AGGGCAAACA GGGCCTGGAT 
GGACAGCAGG GCCAGGAAGG CGAGAGAGGG GAGCTGGGGC CTCGTGGCTT CCCGGGAATC 
CCGGGTCCTT CCGGCCCCCC GGGGGCTAAG GGCGTTCCAG GAGAAGCAGG CCCTCAAGGA 
CCACAGGGAC TGATTGGCCC ATTGGGAGAG ATTGGACCTA AGGGACCCCC TGGTGCTTTG 
GGAGAGCCTG GCCTTCCAGG TGAAGCTGGG ATGAAGGGCG ATTTTGGACC TTTGGGCATC 
CCTGGAGAGC AAGGTCTGGT TGGCCAGCGG GGAGAGACAG GGCTGGAAGG AGATAGTGGC 
CCAGCTGGAC CTGATGGTTT GAAGGGAGAC AAGGGTGAGC CAGGCCCAGA TGGCGAGCGA 
GGAGAGAAAG GCAAAGAAGG TCTGAAGGGC AAAGAAGGTC CCCCTGGCTA TCCTGGGATC 
ACAGGTGTCC GGGGTCCAGA AGGAAAACCA GGCAAACAAG GCGAGAAGGG ACGAACCGGA 
CCTAAGGGTG CCAAGGGCTA CCAAGGGCAT CTTGGTGAGA TGGGTGTTCC AGGAGAGGCC 
GGGCCAGCTG GCAACCAAGG CCCAAAAGGA TCCCGAGGGA CCCTGGGACC AACGGGTGCT 
CCAGGAAGAA TGGGAGCTCA AGGAGAACCT GGATTGCCTG GTTATGATGG ACATAAAGGA 
CTATCAGGAC CCATTGGACC TCCCGGGCCA AAAGGCGAGA AGGGAGAACA GGGTGATGAT 
GGGAAGATTG AGGGCCCTCC AGGGCCTCCT GGAGATCGGG GTCCAGTGGG TGATCGTGGA 
GACCGAGGGG AGCCTGGAGA TCCTGGATAC CCTGGCCAGG AGGGTTTTGA AGGAAGCAGA 
GGAAAAGCTG GTCAGCAAGG CTTACCGGGA CATCCTGGAC CCCGGGGACG ACCAGGACCC 
AAAGGATCAA AGGGTGAAGA GGGTCAGAAA GGGAAACAAG GAAAGCGTGG AGAAGCTGGC 
AGTCGAGGAA TGCAGGGCCT GGTGGGGCAG CCAGGGCCTC GAGGTGTGGT AGGAAGACAA 
GGCCAAGAAG GACCCCCAGG TCAGGATGGA ATTCCTGGGA AGGATGGTCG AGCTGGACAT 
CAGGGGGAAC AAGGAGATGA TGGAGAAGTT GGACCCATTG GCCCTGCAGG AAGGAGAGGA 
AATCCAGGTG TGGCTGGTTT GCCTGGAGCT CAGGGACCCC CAGGATTCAA GGGTGAAAGT 
GGGTTGCCTG GACAACTGGG CCCTACTGGC AAGCGAGGGA CAGAGGGAGG AACAGGGTTC 
CCTGGGAGCC AAGGGGAGCC AGGATCCAAA GGCCAGCCGG GTGACTTTGG AGAAATGGGC 
TTTCCTGGGA TTGCTGGCCT CTTTGGACCC AAGGGCCCTC CAGGAGATAT TGGTTTCAAA 
GGAATCCAGG GGCCCCGGGG ACCACCAGGT TTAATGGGAA AAGAAGGTGT CATTGGCCCC 
TTGGGGATCC TAGGACCGTC TGGAATTCCG GGTCCAAAAG GGAACAAAGG AAGCCGAGGA 
GATTTGGGAT TGCAAGGCCC AAGGGGTCCT CCTGGCCCCA GAGGACGTCC TGGCCCACCG 
GGCCCTCCTG GGATCCCCAT CTCATTTCAG CAAGATGACC TCGGAGCAGC TTTTCAGACG 
TGGATGAACT CCAACAGTGC ACTTAGGACA GAGGGCTACT CCCATTCAGA CCTCCTAGTG 
TTGGACCAGG GTGGGGAGAT CTTTAAGACC TTACACTACC TCAGCAGCCT CATCCAGAGC 
ATCAAGAAGC CTCTAGGAAC CAAGGAGACC CCAGCCCGTA TCTGTCGGGA CCTTATGAAC 
TGTGAGCAGA AGATGGCCGA TGGTACATAT TGGATCGACC CAAATCTTGG CTGTGCTTCA 
GACACCATCG AAGTCACCTG CAATTTTACT AAAGGTGGAC AGACATGTCT CAGGCCCATC 
ACTGCTTCAA AGGTGGACTT TGCCATTGGC CGGGTCCAGA TGAATTTTCT TCACCTGCTG 
AGCTCGGAGG TGATCCAGCA CATCACTATC CACTGCCTCA ACATGTCTGT ATGGCAGGAG 
GGGACAGGCA AGGTGTCCCC CAAACAAGCC GTGCGCTTCC GAGCCTGGAA TGGGCAGATC 
TTTGAAGCGG GCGGCCAGTT TGAGCCAGAG GTGTCCTCAG ATGATTGCAA GGTTCAGGAT 
GGCCGCTGGC ACCAGACCCT GTTCATTTTC CGGACCCAAG ACCCTCACCA GCTGCCCATC 
GTCAGCGTCT ACAACCTCCC TCCAGCCACC CCTGGGAGGC AATACCGCCT GGAAGTTGGA 
CCTGCGTGCT TCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023350019.1
CDS	47..5704
Translation	MEPESDRVSRVRAAASRGGFLLAWIAFCSTCGLAITQGDPEDVDVLQRLGLGGKKLPGGRAPGLAPIPPGVIPFQSGFIFTQRARIEALTGAIIPTTFGTDLTLVLSLCSHRVNNAFLFAVRSRRKKLQLGLQFIPGKIVLFLGQRRSLAFSYDVHDGRWHHLAISLRGQGATLLTSCGKHRVPMDLHFKKEDSLDPEGSFLFGKMNQHSVQFEGAVCQFNIYPSAKAAHNYCKYLRKQCRQADTYRPLLPPLLPLLPQDSEAHTHLGLQPSTSLQSLRNLTTAMPSLAPLRMAKTTPALPRAPGQGARATWVPSAPAGLGAPPHPPVRTSAADPRASRSPTVGTLVWTLPATAKPLPFSSGKASSPGLRPALITPQSNLTKSPVRAMKPMPPSKASVVKTPLVPSKPVTPSVTVVPAKRPHPLVKPVLTSPAKKPVPSGSQPGSAKAPLPPTAKPARQNPGTAKAPPSTAKPVLPAGRSSLPVTPMTASQAARANVAKNLPFTSKPAKLTHPSIKSSLPASGPSLTQTPRPPAKPVLPTAGIKLSRTIPPTFSPGSPPGLNSTGDRKVVEPAVKKELTPKSTPQPPSHPSPRRTGAVPSTVSFPSGLKNLTTWPSPKQAQLSRTAAPVLPQHPASSSRFVGAVTLTHTDGYLFFDPIGPTLFPLLLGPPGQKGDPGLPGPPGLPGLPGISGRRGPRGQKGDPGLSPGKAYDGDKGDVGLPGLTGIPGPTGRKGLKGYPGPPGHPGDQGEPGPEGSLGAKGYPGRQGLPGPIGEPGPKGSRGYIGLPGLFGLPGLDGERGPPGAHGKRGKMGRPGFPGDFGERGPPGLDGNPGELGAPGPPGVHGLIGDMGAIGPIGYPGPKGMKGLMGNIGEHGLKGDKGEQGLPGVPGDIGFQGDKGSQGISGLPGARGKPGPTGKVGDKGPVGFPGPPGPEGFPGDIGPPGQNGPEGAKGKPGARGLPGPRGQLGQEGDEGPTGPPGSPGLEGQPGRKGFPGRPGPDGPKGEPGNSGRPGRMGEQGLMGFIGLVGEPGIVGEKGSRGAMGPPGAPGAKGSMGHPGSAGGVGDPGDPGPPGPPGPRGPLGMRGTKGRRGPRGPDGPAGEPGSKGVKGPPGTQGKQGLDGQQGQEGERGELGPRGFPGIPGPSGPPGAKGVPGEAGPQGPQGLIGPLGEIGPKGPPGALGEPGLPGEAGMKGDFGPLGIPGEQGLVGQRGETGLEGDSGPAGPDGLKGDKGEPGPDGERGEKGKEGLKGKEGPPGYPGITGVRGPEGKPGKQGEKGRTGPKGAKGYQGHLGEMGVPGEAGPAGNQGPKGSRGTLGPTGAPGRMGAQGEPGLPGYDGHKGLSGPIGPPGPKGEKGEQGDDGKIEGPPGPPGDRGPVGDRGDRGEPGDPGYPGQEGFEGSRGKAGQQGLPGHPGPRGRPGPKGSKGEEGQKGKQGKRGEAGSRGMQGLVGQPGPRGVVGRQGQEGPPGQDGIPGKDGRAGHQGEQGDDGEVGPIGPAGRRGNPGVAGLPGAQGPPGFKGESGLPGQLGPTGKRGTEGGTGFPGSQGEPGSKGQPGDFGEMGFPGIAGLFGPKGPPGDIGFKGIQGPRGPPGLMGKEGVIGPLGILGPSGIPGPKGNKGSRGDLGLQGPRGPPGPRGRPGPPGPPGIPISFQQDDLGAAFQTWMNSNSALRTEGYSHSDLLVLDQGGEIFKTLHYLSSLIQSIKKPLGTKETPARICRDLMNCEQKMADGTYWIDPNLGCASDTIEVTCNFTKGGQTCLRPITASKVDFAIGRVQMNFLHLLSSEVIQHITIHCLNMSVWQEGTGKVSPKQAVRFRAWNGQIFEAGGQFEPEVSSDDCKVQDGRWHQTLFIFRTQDPHQLPIVSVYNLPPATPGRQYRLEVGPACFL

Target ORF information:

RefSeq Version	XM_023494251.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii collagen type XXVII alpha 1 chain (COL27A1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023494251.1

ORF Insert Sequence:

ATGGAGCCGG AATCCGACCG GGTCTCCAGA GTCAGAGCAG CTGCGTCCAG GGGGGGATTC 
CTCCTAGCCT GGATTGCCTT CTGCTCTACC TGTGGCCTGG CCATCACCCA AGGAGATCCT 
GAAGATGTGG ATGTCCTGCA ACGGCTGGGC CTCGGGGGGA AGAAGTTGCC CGGCGGCCGG 
GCCCCAGGGC TGGCCCCCAT CCCCCCAGGA GTCATCCCGT TCCAGTCGGG GTTCATCTTC 
ACCCAGCGGG CCCGGATTGA AGCCCTTACA GGCGCCATCA TTCCCACCAC CTTCGGCACT 
GACCTGACCC TGGTGCTGAG CCTGTGCTCC CACCGAGTCA ACAACGCCTT CCTCTTTGCT 
GTCCGGAGCC GCAGGAAGAA GCTGCAGCTG GGGCTCCAGT TCATCCCTGG CAAGATCGTG 
CTGTTCCTGG GCCAGCGGCG CTCCCTGGCC TTCAGCTACG ACGTGCATGA CGGGCGATGG 
CACCACTTGG CCATCAGTCT CCGAGGCCAG GGGGCCACCC TCCTCACCTC CTGCGGGAAG 
CACCGCGTGC CCATGGATCT GCATTTTAAG AAAGAGGACT CTCTTGATCC CGAGGGGTCT 
TTCCTCTTTG GGAAGATGAA TCAGCACTCA GTCCAATTTG AGGGCGCCGT ATGCCAGTTC 
AATATTTATC CTTCTGCCAA GGCAGCTCAC AATTATTGTA AATATCTGAG GAAGCAGTGC 
AGACAGGCCG ACACCTACCG GCCACTGCTG CCCCCTCTTC TTCCCCTCCT TCCCCAGGAC 
TCCGAGGCCC ATACCCATCT TGGCCTCCAG CCCAGCACCT CTCTGCAGAG CCTGAGGAAT 
CTGACCACTG CGATGCCTTC GCTGGCACCC CTTAGAATGG CCAAGACCAC CCCGGCCTTG 
CCCAGAGCCC CCGGCCAAGG TGCCAGGGCT ACATGGGTGC CCTCAGCACC GGCCGGGCTT 
GGGGCACCCC CCCACCCGCC TGTGAGGACT AGTGCCGCGG ATCCCCGGGC GTCCCGATCC 
CCCACAGTGG GTACTTTGGT CTGGACCCTA CCAGCAACTG CAAAGCCATT GCCTTTCTCT 
TCAGGCAAAG CTTCCTCACC TGGGCTCAGG CCTGCACTGA TCACCCCTCA GAGCAACTTG 
ACCAAATCTC CCGTTCGCGC CATGAAGCCC ATGCCCCCAT CCAAGGCCAG TGTGGTTAAA 
ACTCCCCTTG TTCCCAGTAA GCCAGTAACT CCTTCAGTCA CTGTGGTCCC AGCTAAGAGG 
CCCCATCCCC TGGTCAAGCC GGTGCTGACC TCCCCTGCTA AGAAGCCAGT GCCCTCTGGC 
TCTCAGCCTG GCTCTGCCAA AGCCCCCCTT CCTCCCACAG CGAAACCAGC CCGTCAGAAC 
CCTGGAACAG CCAAAGCTCC TCCTTCCACT GCCAAGCCAG TGCTTCCCGC CGGGCGGTCC 
TCCCTTCCCG TTACCCCCAT GACAGCGTCC CAGGCTGCTC GGGCCAACGT GGCCAAAAAC 
CTCCCCTTTA CCAGCAAGCC GGCCAAGCTG ACCCACCCTT CCATTAAGTC ATCCTTGCCA 
GCCTCAGGTC CGAGCCTAAC CCAGACCCCT CGGCCCCCAG CAAAGCCGGT GCTTCCTACC 
GCGGGGATCA AATTGTCCAG AACAATCCCT CCCACCTTCT CCCCAGGCTC TCCTCCTGGG 
CTCAACTCCA CTGGTGACAG AAAAGTGGTT GAGCCCGCTG TGAAGAAGGA ATTGACTCCA 
AAGAGCACCC CCCAGCCACC CAGCCATCCC AGCCCTAGGA GGACTGGAGC AGTCCCTAGT 
ACCGTCTCCT TCCCTTCAGG CCTGAAGAAC CTGACCACGT GGCCCAGCCC CAAACAGGCT 
CAGCTCAGCC GTACAGCTGC CCCGGTTCTC CCACAGCACC CAGCCTCCAG CTCCCGTTTT 
GTGGGCGCCG TCACTCTCAC TCACACAGAT GGATACCTGT TCTTTGATCC AATTGGGCCC 
ACCTTGTTCC CACTGCTGTT GGGCCCTCCA GGGCAGAAGG GAGACCCTGG CCTCCCGGGC 
CCCCCTGGCT TGCCTGGGTT GCCTGGAATA TCAGGCAGAA GAGGACCCCG GGGACAAAAA 
GGAGATCCTG GGCTTTCCCC TGGAAAGGCC TATGATGGTG ACAAGGGAGA CGTAGGCTTA 
CCAGGACTGA CTGGGATTCC GGGACCAACT GGCCGGAAGG GGCTGAAAGG CTATCCTGGA 
CCACCTGGGC ATCCTGGAGA CCAGGGAGAG CCAGGACCAG AAGGGAGTCT GGGAGCCAAA 
GGTTATCCTG GCAGGCAGGG ATTACCTGGG CCAATAGGAG AACCTGGACC CAAAGGCAGT 
CGGGGGTATA TTGGTCTCCC CGGGCTCTTT GGCCTGCCAG GACTTGATGG AGAAAGGGGG 
CCTCCTGGAG CTCATGGCAA GAGGGGCAAG ATGGGTAGGC CGGGATTTCC TGGTGACTTT 
GGGGAAAGAG GGCCCCCCGG ATTGGATGGC AACCCCGGAG AGCTGGGTGC CCCAGGCCCA 
CCTGGAGTTC ATGGACTTAT CGGTGACATG GGAGCTATTG GTCCAATTGG CTACCCAGGG 
CCCAAGGGAA TGAAGGGGCT TATGGGAAAC ATCGGAGAAC ACGGACTGAA AGGTGATAAG 
GGCGAGCAAG GGCTGCCTGG TGTGCCTGGA GATATCGGAT TCCAAGGAGA TAAGGGGAGT 
CAGGGAATAT CAGGGTTACC GGGTGCTCGA GGAAAGCCAG GTCCTACAGG CAAGGTTGGT 
GACAAAGGAC CAGTTGGGTT TCCTGGGCCT CCAGGTCCTG AGGGATTCCC AGGGGACATA 
GGACCACCAG GCCAGAATGG TCCAGAAGGT GCGAAGGGCA AGCCAGGAGC CCGAGGCTTA 
CCAGGACCGA GAGGACAGCT TGGGCAGGAG GGAGATGAGG GACCAACGGG ACCACCAGGA 
TCCCCTGGCT TAGAGGGTCA ACCTGGCCGG AAAGGATTTC CAGGAAGGCC CGGCCCTGAT 
GGCCCCAAGG GTGAGCCAGG AAACTCGGGT CGGCCTGGAA GAATGGGTGA GCAGGGCCTG 
ATGGGATTTA TCGGTCTGGT CGGGGAGCCA GGAATCGTTG GAGAGAAGGG TAGCCGAGGT 
GCTATGGGAC CACCAGGTGC TCCTGGAGCC AAGGGGTCGA TGGGTCATCC TGGTTCAGCA 
GGTGGAGTTG GAGATCCTGG AGACCCCGGG CCCCCAGGAC CTCCTGGACC TCGTGGGCCA 
CTGGGGATGC GAGGAACAAA AGGACGTCGG GGGCCCCGAG GACCAGATGG ACCAGCAGGG 
GAGCCAGGGT CAAAAGGTGT CAAGGGCCCT CCAGGAACAC AGGGCAAACA GGGCCTGGAT 
GGACAGCAGG GCCAGGAAGG CGAGAGAGGG GAGCTGGGGC CTCGTGGCTT CCCGGGAATC 
CCGGGTCCTT CCGGCCCCCC GGGGGCTAAG GGCGTTCCAG GAGAAGCAGG CCCTCAAGGA 
CCACAGGGAC TGATTGGCCC ATTGGGAGAG ATTGGACCTA AGGGACCCCC TGGTGCTTTG 
GGAGAGCCTG GCCTTCCAGG TGAAGCTGGG ATGAAGGGCG ATTTTGGACC TTTGGGCATC 
CCTGGAGAGC AAGGTCTGGT TGGCCAGCGG GGAGAGACAG GGCTGGAAGG AGATAGTGGC 
CCAGCTGGAC CTGATGGTTT GAAGGGAGAC AAGGGTGAGC CAGGCCCAGA TGGCGAGCGA 
GGAGAGAAAG GCAAAGAAGG TCTGAAGGGC AAAGAAGGTC CCCCTGGCTA TCCTGGGATC 
ACAGGTGTCC GGGGTCCAGA AGGAAAACCA GGCAAACAAG GCGAGAAGGG ACGAACCGGA 
CCTAAGGGTG CCAAGGGCTA CCAAGGGCAT CTTGGTGAGA TGGGTGTTCC AGGAGAGGCC 
GGGCCAGCTG GCAACCAAGG CCCAAAAGGA TCCCGAGGGA CCCTGGGACC AACGGGTGCT 
CCAGGAAGAA TGGGAGCTCA AGGAGAACCT GGATTGCCTG GTTATGATGG ACATAAAGGA 
CTATCAGGAC CCATTGGACC TCCCGGGCCA AAAGGCGAGA AGGGAGAACA GGGTGATGAT 
GGGAAGATTG AGGGCCCTCC AGGGCCTCCT GGAGATCGGG GTCCAGTGGG TGATCGTGGA 
GACCGAGGGG AGCCTGGAGA TCCTGGATAC CCTGGCCAGG AGGGTTTTGA AGGAAGCAGA 
GGAAAAGCTG GTCAGCAAGG CTTACCGGGA CATCCTGGAC CCCGGGGACG ACCAGGACCC 
AAAGGATCAA AGGGTGAAGA GGGTCAGAAA GGGAAACAAG GAAAGCGTGG AGAAGCTGGC 
AGTCGAGGAA TGCAGGGCCT GGTGGGGCAG CCAGGGCCTC GAGGTGTGGT AGGAAGACAA 
GGCCAAGAAG GACCCCCAGG TCAGGATGGA ATTCCTGGGA AGGATGGTCG AGCTGGACAT 
CAGGGGGAAC AAGGAGATGA TGGAGAAGTT GGACCCATTG GCCCTGCAGG AAGGAGAGGA 
AATCCAGGTG TGGCTGGTTT GCCTGGAGCT CAGGGACCCC CAGGATTCAA GGGTGAAAGT 
GGGTTGCCTG GACAACTGGG CCCTACTGGC AAGCGAGGGA CAGAGGGAGG AACAGGGTTC 
CCTGGGAGCC AAGGGGAGCC AGGATCCAAA GGCCAGCCGG GTGACTTTGG AGAAATGGGC 
TTTCCTGGGA TTGCTGGCCT CTTTGGACCC AAGGGCCCTC CAGGAGATAT TGGTTTCAAA 
GGAATCCAGG GGCCCCGGGG ACCACCAGGT TTAATGGGAA AAGAAGGTGT CATTGGCCCC 
TTGGGGATCC TAGGACCGTC TGGAATTCCG GGTCCAAAAG GGAACAAAGG AAGCCGAGGA 
GATTTGGGAT TGCAAGGCCC AAGGGGTCCT CCTGGCCCCA GAGGACGTCC TGGCCCACCG 
GGCCCTCCTG GGATCCCCAT CTCATTTCAG CAAGATGACC TCGGAGCAGC TTTTCAGACG 
TGGATGAACT CCAACAGTGC ACTTAGGACA GAGGGCTACT CCCATTCAGA CCTCCTAGTG 
TTGGACCAGG GTGGGGAGAT CTTTAAGACC TTACACTACC TCAGCAGCCT CATCCAGAGC 
ATCAAGAAGC CTCTAGGAAC CAAGGAGACC CCAGCCCGTA TCTGTCGGGA CCTTATGAAC 
TGTGAGCAGA AGATGGCCGA TGGTACATAT TGGATCGACC CAAATCTTGG CTGTGCTTCA 
GACACCATCG AAGTCACCTG CAATTTTACT AAAGGTGGAC AGACATGTCT CAGGCCCATC 
ACTGCTTCAA AGGTGGACTT TGCCATTGGC CGGGTCCAGA TGAATTTTCT TCACCTGCTG 
AGCTCGGAGG TGATCCAGCA CATCACTATC CACTGCCTCA ACATGTCTGT ATGGCAGGAG 
GGGACAGGCA AGGTGTCCCC CAAACAAGCC GTGCGCTTCC GAGCCTGGAA TGGGCAGATC 
TTTGAAGCGG GCGGCCAGTT TGAGCCAGAG GTGTCCTCAG ATGATTGCAA GGTTCAGGAT 
GGCCGCTGGC ACCAGACCCT GTTCATTTTC CGGACCCAAG ACCCTCACCA GCTGCCCATC 
GTCAGCGTCT ACAACCTCCC TCCAGCCACC CCTGGGAGGC AATACCGCCT GGAAGTTGGA 
CCTGCGTGCT TCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL27A1 ( XM_023494252.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_023350020.1 Sarcophilus harrisii collagen type XXVII alpha 1 chain (COL27A1), transcript variant X3, mRNA.

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CloneID	OSa150308
Clone ID Related Accession (Same CDS sequence)	XM_023494252.1
Accession Version	XM_023494252.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5403bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515513600000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	collagen alpha-1(XXVII) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003816661.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCGG AATCCGACCG GGTCTCCAGA GTCAGAGCAG CTGCGTCCAG GGGGGGATTC 
CTCCTAGCCT GGATTGCCTT CTGCTCTACC TGTGGCCTGG CCATCACCCA AGGAGATCCT 
GAAGATGTGG ATGTCCTGCA ACGGCTGGGC CTCGGGGGGA AGAAGTTGCC CGGCGGCCGG 
GCCCCAGGGC TGGCCCCCAT CCCCCCAGGA GTCATCCCGT TCCAGTCGGG GTTCATCTTC 
ACCCAGCGGG CCCGGATTGA AGCCCTTACA GGCGCCATCA TTCCCACCAC CTTCGGCACT 
GACCTGACCC TGGTGCTGAG CCTGTGCTCC CACCGAGTCA ACAACGCCTT CCTCTTTGCT 
GTCCGGAGCC GCAGGAAGAA GCTGCAGCTG GGGCTCCAGT TCATCCCTGG CAAGATCGTG 
CTGTTCCTGG GCCAGCGGCG CTCCCTGGCC TTCAGCTACG ACGTGCATGA CGGGCGATGG 
CACCACTTGG CCATCAGTCT CCGAGGCCAG GGGGCCACCC TCCTCACCTC CTGCGGGAAG 
CACCGCGTGC CCATGGATCT GCATTTTAAG AAAGAGGACT CTCTTGATCC CGAGGGGTCT 
TTCCTCTTTG GGAAGATGAA TCAGCACTCA GTCCAATTTG AGGGCGCCGT ATGCCAGTTC 
AATATTTATC CTTCTGCCAA GGCAGCTCAC AATTATTGTA AATATCTGAG GAAGCAGTGC 
AGACAGGCCG ACACCTACCG GCCACTGCTG CCCCCTCTTC TTCCCCTCCT TCCCCAGGAC 
TCCGAGGCCC ATACCCATCT TGGCCTCCAG CCCAGCACCT CTCTGCAGAG CCTGAGGAAT 
CTGACCACTG CGATGCCTTC GCTGGCACCC CTTAGAATGG CCAAGACCAC CCCGGCCTTG 
CCCAGAGCCC CCGGCCAAGG TGCCAGGGCT ACATGGGTGC CCTCAGCACC GGCCGGGCTT 
GGGGCACCCC CCCACCCGCC TGTGAGGACT AGTGCCGCGG ATCCCCGGGC GTCCCGATCC 
CCCACAGTGG GTACTTTGGT CTGGACCCTA CCAGCAACTG CAAAGCCATT GCCTTTCTCT 
TCAGGCAAAG CTTCCTCACC TGGGCTCAGG CCTGCACTGA TCACCCCTCA GAGCAACTTG 
ACCAAATCTC CCGTTCGCGC CATGAAGCCC ATGCCCCCAT CCAAGGCCAG TGTGGTTAAA 
ACTCCCCTTG TTCCCAGTAA GCCAGTAACT CCTTCAGTCA CTGTGGTCCC AGCTAAGAGG 
CCCCATCCCC TGGTCAAGCC GGTGCTGACC TCCCCTGCTA AGAAGCCAGT GCCCTCTGGC 
TCTCAGCCTG GCTCTGCCAA AGCCCCCCTT CCTCCCACAG CGAAACCAGC CCGTCAGAAC 
CCTGGAACAG CCAAAGCTCC TCCTTCCACT GCCAAGCCAG TGCTTCCCGC CGGGCGGTCC 
TCCCTTCCCG TTACCCCCAT GACAGCGTCC CAGGCTGCTC GGGCCAACGT GGCCAAAAAC 
CTCCCCTTTA CCAGCAAGCC GGCCAAGCTG ACCCACCCTT CCATTAAGTC ATCCTTGCCA 
GCCTCAGGTC CGAGCCTAAC CCAGACCCCT CGGCCCCCAG CAAAGCCGGT GCTTCCTACC 
GCGGGGATCA AATTGTCCAG AACAATCCCT CCCACCTTCT CCCCAGGCTC TCCTCCTGGG 
CTCAACTCCA CTGGTGACAG AAAAGTGGTT GAGCCCGCTG TGAAGAAGGA ATTGACTCCA 
AAGAGCACCC CCCAGCCACC CAGCCATCCC AGCCCTAGGA GGACTGGAGC AGTCCCTAGT 
ACCGTCTCCT TCCCTTCAGG CCTGAAGAAC CTGACCACGT GGCCCAGCCC CAAACAGGCT 
CAGCTCAGCC GTACAGCTGC CCCGGTTCTC CCACAGCACC CAGCCTCCAG CTCCCGTTTT 
GTGGGCGCCG TCACTCTCAC TCACACAGAT GGATACCTGT TCTTTGATCC AATTGGGCCC 
ACCTTGTTCC CACTGCTGTT GGGCCCTCCA GGGCAGAAGG GAGACCCTGG CCTCCCGGGC 
CCCCCTGGCT TGCCTGGGTT GCCTGGAATA TCAGGCAGAA GAGGACCCCG GGGTCCCCCT 
GGGCCTCATG GAAACCCTGG GCCTCCTGGC TCTCCTGGTG CAAAGGGACA AAAAGGAGAT 
CCTGGGCTTT CCCCTGGAAA GGCCTATGAT GGTGACAAGG GAGACGTAGG CTTACCAGGA 
CTGACTGGGA TTCCGGGACC AACTGGCCGG AAGGGGCTGA AAGGCTATCC TGGACCACCT 
GGGCATCCTG GAGACCAGGG AGAGCCAGGA CCAGAAGGGA GTCTGGGAGC CAAAGGTTAT 
CCTGGCAGGC AGGGATTACC TGGGCCAATA GGAGAACCTG GACCCAAAGG CAGTCGGGGG 
TATATTGGTC TCCCCGGGCT CTTTGGCCTG CCAGGACTTG ATGGAGAAAG GGGGCCTCCT 
GGAGCTCATG GCAAGAGGGG CAAGATGGGT AGGCCGGGAT TTCCTGGTGA CTTTGGGGAA 
AGAGGGCCCC CCGGATTGGA TGGCAACCCC GGAGAGCTGG GTGCCCCAGG CCCACCTGGA 
GTTCATGGAC TTATCGGTGA CATGGGAGCT ATTGGTCCAA TTGGCTACCC AGGGCCCAAG 
GGAATGAAGG GGCTTATGGG AAACATCGGA GAACACGGAC TGAAAGGTGA TAAGGGCGAG 
CAAGGGCTGC CTGGTGTGCC TGGAGATATC GGATTCCAAG GAGATAAGGG GAGTCAGGGA 
ATATCAGGGT TACCGGGTGC TCGAGGAAAG CCAGGTCCTA CAGGCAAGGT TGGTGACAAA 
GGACCAGTTG GGTTTCCTGG GCCTCCAGGT CCTGAGGGAT TCCCAGGGGA CATAGGACCA 
CCAGGCCAGA ATGGTCCAGA AGGTGCGAAG GGCAAGCCAG GAGCCCGAGG CTTACCAGGA 
CCGAGAGGAC AGCTTGGGCA GGAGGGAGAT GAGGGACCAA CGGGACCACC AGGATCCCCT 
GGCTTAGAGG GTCAACCTGG CCGGAAAGGA TTTCCAGGAA GGCCCGGCCC TGATGGCCCC 
AAGGGTGAGC CAGGAAACTC GGGTCGGCCT GGAAGAATGG GTGAGCAGGG CCTGATGGGA 
TTTATCGGTC TGGTCGGGGA GCCAGGAATC GTTGGAGAGA AGGGTAGCCG AGGTGCTATG 
GGACCACCAG GTGCTCCTGG AGCCAAGGGG TCGATGGGTC ATCCTGGTTC AGCAGGTGGA 
GTTGGAGATC CTGGAGACCC CGGGCCCCCA GGACCTCCTG GACCTCGTGG GCCACTGGGG 
ATGCGAGGAA CAAAAGGACG TCGGGGGCCC CGAGGACCAG ATGGACCAGC AGGGGAGCCA 
GGGTCAAAAG GTGTCAAGGG CCCTCCAGGA ACACAGGGCA AACAGGGCCT GGATGGACAG 
CAGGGCCAGG AAGGCGAGAG AGGGGAGCTG GGGCCTCGTG GCTTCCCGGG AATCCCGGGT 
CCTTCCGGCC CCCCGGGGGC TAAGGGCGTT CCAGGAGAAG CAGGCCCTCA AGGACCACAG 
GGACTGATTG GCCCATTGGG AGAGATTGGA CCTAAGGGAC CCCCTGGTGC TTTGGGAGAG 
CCTGGCCTTC CAGGTGAAGC TGGGATGAAG GGCGATTTTG GACCTTTGGG CATCCCTGGA 
GAGCAAGGTC TGGTTGGCCA GCGGGGAGAG ACAGGGCTGG AAGGAGATAG TGGCCCAGCT 
GGACCTGATG GTTTGAAGGG AGACAAGGGT GAGCCAGGCC CAGATGGCGA GCGAGGAGAG 
AAAGGCAAAG AAGGTCTGAA GGGCAAAGAA GGTCCCCCTG GCTATCCTGG GATCACAGGT 
GTCCGGGGTC CAGAAGGAAA ACCAGGCAAA CAAGGCGAGA AGGGACGAAC CGGACCTAAG 
GGTGCCAAGG GCTACCAAGG GCATCTTGGT GAGATGGGTG TTCCAGGAGA GGCCGGGCCA 
GCTGGCAACC AAGGCCCAAA AGGATCCCGA GGGACCCTGG GACCAACGGG TGCTCCAGGA 
AGAATGGGAG CTCAAGGAGA ACCTGGATTG CCTGGTTATG ATGGACATAA AGGACTATCA 
GGACCCATTG GACCTCCCGG GCCAAAAGGC GAGAAGGGAG AACAGGGTGA TGATGGGAAG 
ATTGAGGGCC CTCCAGGGCC TCCTGGAGAT CGGGGTCCAG TGGGTGATCG TGGAGACCGA 
GGGGAGCCTG GAGATCCTGG ATACCCTGGC CAGGAGGGTT TTGAAGGAAG CAGAGGAAAA 
GCTGGTCAGC AAGGCTTACC GGGACATCCT GGACCCCGGG GACGACCAGG ACCCAAAGGA 
TCAAAGGGTG AAGAGGGTCA GAAAGGGAAA CAAGGAAAGC GTGGAGAAGC TGGCAGTCGA 
GGAATGCAGG GCCTGGTGGG GCAGCCAGGG CCTCGAGGTG TGGTAGGAAG ACAAGGCCAA 
GAAGGACCCC CAGGTCAGGA TGGAATTCCT GGGAAGGATG GTCGAGCTGG ACATCAGGGG 
GAACAAGGAG ATGATGGAGA AGTTGGACCC ATTGGCCCTG CAGGAAGGAG AGGAAATCCA 
GGTGTGGCTG GTTTGCCTGG AGCTCAGGGA CCCCCAGGAT TCAAGGGTGA AAGTGGGTTG 
CCTGGACAAC TGGGCCCTAC TGGCAAGCGA GGGACAGAGG GAGGAACAGG GTTCCCTGGG 
AGCCAAGGGG AGCCAGGATC CAAAGGCCAG CCGGGTGACT TTGGAGAAAT GGGCTTTCCT 
GGGATTGCTG GCCTCTTTGG ACCCAAGGGC CCTCCAGGAG ATATTGGTTT CAAAGGAATC 
CAGGGGCCCC GGGGACCACC AGGTTTAATG GGAAAAGAAG GTGTCATTGG CCCCTTGGGG 
ATCCTAGGAC CGTCTGGAAT TCCGGGTCCA AAAGGGAACA AAGGAAGCCG AGGAGATTTG 
GGATTGCAAG GCCCAAGGGG TCCTCCTGGC CCCAGAGGAC GTCCTGGCCC ACCGGGCCCT 
CCTGGGATCC CCATCTCATT TCAGCAAGAT GACCTCGGAG CAGCTTTTCA GACGTGGATG 
AACTCCAACA GTGCACTTAG GACAGAGGGC TACTCCCATT CAGACCTCCT AGTGTTGGAC 
CAGGGTGGGG AGATCTTTAA GACCTTACAC TACCTCAGCA GCCTCATCCA GAGCATCAAG 
AAGCCTCTAG GAACCAAGGA GACCCCAGCC CGTATCTGTC GGGACCTTAT GAACTGTGAG 
CAGAAGATGG CCGATGGTAC ATATTGGATC GACCCAAATC TTGGCTGTGC TTCAGACACC 
ATCGAAGTCA CCTGCAATTT TACTAAAGGT GGACAGACAT GTCTCAGGCC CATCACTGCT 
TCAAAGAGCT CATTACTCTC GGGTGCTTCC AGGTGGACTT TGCCATTGGC CGGGTCCAGA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023350020.1
CDS	47..5449
Translation	MEPESDRVSRVRAAASRGGFLLAWIAFCSTCGLAITQGDPEDVDVLQRLGLGGKKLPGGRAPGLAPIPPGVIPFQSGFIFTQRARIEALTGAIIPTTFGTDLTLVLSLCSHRVNNAFLFAVRSRRKKLQLGLQFIPGKIVLFLGQRRSLAFSYDVHDGRWHHLAISLRGQGATLLTSCGKHRVPMDLHFKKEDSLDPEGSFLFGKMNQHSVQFEGAVCQFNIYPSAKAAHNYCKYLRKQCRQADTYRPLLPPLLPLLPQDSEAHTHLGLQPSTSLQSLRNLTTAMPSLAPLRMAKTTPALPRAPGQGARATWVPSAPAGLGAPPHPPVRTSAADPRASRSPTVGTLVWTLPATAKPLPFSSGKASSPGLRPALITPQSNLTKSPVRAMKPMPPSKASVVKTPLVPSKPVTPSVTVVPAKRPHPLVKPVLTSPAKKPVPSGSQPGSAKAPLPPTAKPARQNPGTAKAPPSTAKPVLPAGRSSLPVTPMTASQAARANVAKNLPFTSKPAKLTHPSIKSSLPASGPSLTQTPRPPAKPVLPTAGIKLSRTIPPTFSPGSPPGLNSTGDRKVVEPAVKKELTPKSTPQPPSHPSPRRTGAVPSTVSFPSGLKNLTTWPSPKQAQLSRTAAPVLPQHPASSSRFVGAVTLTHTDGYLFFDPIGPTLFPLLLGPPGQKGDPGLPGPPGLPGLPGISGRRGPRGPPGPHGNPGPPGSPGAKGQKGDPGLSPGKAYDGDKGDVGLPGLTGIPGPTGRKGLKGYPGPPGHPGDQGEPGPEGSLGAKGYPGRQGLPGPIGEPGPKGSRGYIGLPGLFGLPGLDGERGPPGAHGKRGKMGRPGFPGDFGERGPPGLDGNPGELGAPGPPGVHGLIGDMGAIGPIGYPGPKGMKGLMGNIGEHGLKGDKGEQGLPGVPGDIGFQGDKGSQGISGLPGARGKPGPTGKVGDKGPVGFPGPPGPEGFPGDIGPPGQNGPEGAKGKPGARGLPGPRGQLGQEGDEGPTGPPGSPGLEGQPGRKGFPGRPGPDGPKGEPGNSGRPGRMGEQGLMGFIGLVGEPGIVGEKGSRGAMGPPGAPGAKGSMGHPGSAGGVGDPGDPGPPGPPGPRGPLGMRGTKGRRGPRGPDGPAGEPGSKGVKGPPGTQGKQGLDGQQGQEGERGELGPRGFPGIPGPSGPPGAKGVPGEAGPQGPQGLIGPLGEIGPKGPPGALGEPGLPGEAGMKGDFGPLGIPGEQGLVGQRGETGLEGDSGPAGPDGLKGDKGEPGPDGERGEKGKEGLKGKEGPPGYPGITGVRGPEGKPGKQGEKGRTGPKGAKGYQGHLGEMGVPGEAGPAGNQGPKGSRGTLGPTGAPGRMGAQGEPGLPGYDGHKGLSGPIGPPGPKGEKGEQGDDGKIEGPPGPPGDRGPVGDRGDRGEPGDPGYPGQEGFEGSRGKAGQQGLPGHPGPRGRPGPKGSKGEEGQKGKQGKRGEAGSRGMQGLVGQPGPRGVVGRQGQEGPPGQDGIPGKDGRAGHQGEQGDDGEVGPIGPAGRRGNPGVAGLPGAQGPPGFKGESGLPGQLGPTGKRGTEGGTGFPGSQGEPGSKGQPGDFGEMGFPGIAGLFGPKGPPGDIGFKGIQGPRGPPGLMGKEGVIGPLGILGPSGIPGPKGNKGSRGDLGLQGPRGPPGPRGRPGPPGPPGIPISFQQDDLGAAFQTWMNSNSALRTEGYSHSDLLVLDQGGEIFKTLHYLSSLIQSIKKPLGTKETPARICRDLMNCEQKMADGTYWIDPNLGCASDTIEVTCNFTKGGQTCLRPITASKSSLLSGASRWTLPLAGSR

Target ORF information:

RefSeq Version	XM_023494252.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii collagen type XXVII alpha 1 chain (COL27A1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023494252.1

ORF Insert Sequence:

ATGGAGCCGG AATCCGACCG GGTCTCCAGA GTCAGAGCAG CTGCGTCCAG GGGGGGATTC 
CTCCTAGCCT GGATTGCCTT CTGCTCTACC TGTGGCCTGG CCATCACCCA AGGAGATCCT 
GAAGATGTGG ATGTCCTGCA ACGGCTGGGC CTCGGGGGGA AGAAGTTGCC CGGCGGCCGG 
GCCCCAGGGC TGGCCCCCAT CCCCCCAGGA GTCATCCCGT TCCAGTCGGG GTTCATCTTC 
ACCCAGCGGG CCCGGATTGA AGCCCTTACA GGCGCCATCA TTCCCACCAC CTTCGGCACT 
GACCTGACCC TGGTGCTGAG CCTGTGCTCC CACCGAGTCA ACAACGCCTT CCTCTTTGCT 
GTCCGGAGCC GCAGGAAGAA GCTGCAGCTG GGGCTCCAGT TCATCCCTGG CAAGATCGTG 
CTGTTCCTGG GCCAGCGGCG CTCCCTGGCC TTCAGCTACG ACGTGCATGA CGGGCGATGG 
CACCACTTGG CCATCAGTCT CCGAGGCCAG GGGGCCACCC TCCTCACCTC CTGCGGGAAG 
CACCGCGTGC CCATGGATCT GCATTTTAAG AAAGAGGACT CTCTTGATCC CGAGGGGTCT 
TTCCTCTTTG GGAAGATGAA TCAGCACTCA GTCCAATTTG AGGGCGCCGT ATGCCAGTTC 
AATATTTATC CTTCTGCCAA GGCAGCTCAC AATTATTGTA AATATCTGAG GAAGCAGTGC 
AGACAGGCCG ACACCTACCG GCCACTGCTG CCCCCTCTTC TTCCCCTCCT TCCCCAGGAC 
TCCGAGGCCC ATACCCATCT TGGCCTCCAG CCCAGCACCT CTCTGCAGAG CCTGAGGAAT 
CTGACCACTG CGATGCCTTC GCTGGCACCC CTTAGAATGG CCAAGACCAC CCCGGCCTTG 
CCCAGAGCCC CCGGCCAAGG TGCCAGGGCT ACATGGGTGC CCTCAGCACC GGCCGGGCTT 
GGGGCACCCC CCCACCCGCC TGTGAGGACT AGTGCCGCGG ATCCCCGGGC GTCCCGATCC 
CCCACAGTGG GTACTTTGGT CTGGACCCTA CCAGCAACTG CAAAGCCATT GCCTTTCTCT 
TCAGGCAAAG CTTCCTCACC TGGGCTCAGG CCTGCACTGA TCACCCCTCA GAGCAACTTG 
ACCAAATCTC CCGTTCGCGC CATGAAGCCC ATGCCCCCAT CCAAGGCCAG TGTGGTTAAA 
ACTCCCCTTG TTCCCAGTAA GCCAGTAACT CCTTCAGTCA CTGTGGTCCC AGCTAAGAGG 
CCCCATCCCC TGGTCAAGCC GGTGCTGACC TCCCCTGCTA AGAAGCCAGT GCCCTCTGGC 
TCTCAGCCTG GCTCTGCCAA AGCCCCCCTT CCTCCCACAG CGAAACCAGC CCGTCAGAAC 
CCTGGAACAG CCAAAGCTCC TCCTTCCACT GCCAAGCCAG TGCTTCCCGC CGGGCGGTCC 
TCCCTTCCCG TTACCCCCAT GACAGCGTCC CAGGCTGCTC GGGCCAACGT GGCCAAAAAC 
CTCCCCTTTA CCAGCAAGCC GGCCAAGCTG ACCCACCCTT CCATTAAGTC ATCCTTGCCA 
GCCTCAGGTC CGAGCCTAAC CCAGACCCCT CGGCCCCCAG CAAAGCCGGT GCTTCCTACC 
GCGGGGATCA AATTGTCCAG AACAATCCCT CCCACCTTCT CCCCAGGCTC TCCTCCTGGG 
CTCAACTCCA CTGGTGACAG AAAAGTGGTT GAGCCCGCTG TGAAGAAGGA ATTGACTCCA 
AAGAGCACCC CCCAGCCACC CAGCCATCCC AGCCCTAGGA GGACTGGAGC AGTCCCTAGT 
ACCGTCTCCT TCCCTTCAGG CCTGAAGAAC CTGACCACGT GGCCCAGCCC CAAACAGGCT 
CAGCTCAGCC GTACAGCTGC CCCGGTTCTC CCACAGCACC CAGCCTCCAG CTCCCGTTTT 
GTGGGCGCCG TCACTCTCAC TCACACAGAT GGATACCTGT TCTTTGATCC AATTGGGCCC 
ACCTTGTTCC CACTGCTGTT GGGCCCTCCA GGGCAGAAGG GAGACCCTGG CCTCCCGGGC 
CCCCCTGGCT TGCCTGGGTT GCCTGGAATA TCAGGCAGAA GAGGACCCCG GGGTCCCCCT 
GGGCCTCATG GAAACCCTGG GCCTCCTGGC TCTCCTGGTG CAAAGGGACA AAAAGGAGAT 
CCTGGGCTTT CCCCTGGAAA GGCCTATGAT GGTGACAAGG GAGACGTAGG CTTACCAGGA 
CTGACTGGGA TTCCGGGACC AACTGGCCGG AAGGGGCTGA AAGGCTATCC TGGACCACCT 
GGGCATCCTG GAGACCAGGG AGAGCCAGGA CCAGAAGGGA GTCTGGGAGC CAAAGGTTAT 
CCTGGCAGGC AGGGATTACC TGGGCCAATA GGAGAACCTG GACCCAAAGG CAGTCGGGGG 
TATATTGGTC TCCCCGGGCT CTTTGGCCTG CCAGGACTTG ATGGAGAAAG GGGGCCTCCT 
GGAGCTCATG GCAAGAGGGG CAAGATGGGT AGGCCGGGAT TTCCTGGTGA CTTTGGGGAA 
AGAGGGCCCC CCGGATTGGA TGGCAACCCC GGAGAGCTGG GTGCCCCAGG CCCACCTGGA 
GTTCATGGAC TTATCGGTGA CATGGGAGCT ATTGGTCCAA TTGGCTACCC AGGGCCCAAG 
GGAATGAAGG GGCTTATGGG AAACATCGGA GAACACGGAC TGAAAGGTGA TAAGGGCGAG 
CAAGGGCTGC CTGGTGTGCC TGGAGATATC GGATTCCAAG GAGATAAGGG GAGTCAGGGA 
ATATCAGGGT TACCGGGTGC TCGAGGAAAG CCAGGTCCTA CAGGCAAGGT TGGTGACAAA 
GGACCAGTTG GGTTTCCTGG GCCTCCAGGT CCTGAGGGAT TCCCAGGGGA CATAGGACCA 
CCAGGCCAGA ATGGTCCAGA AGGTGCGAAG GGCAAGCCAG GAGCCCGAGG CTTACCAGGA 
CCGAGAGGAC AGCTTGGGCA GGAGGGAGAT GAGGGACCAA CGGGACCACC AGGATCCCCT 
GGCTTAGAGG GTCAACCTGG CCGGAAAGGA TTTCCAGGAA GGCCCGGCCC TGATGGCCCC 
AAGGGTGAGC CAGGAAACTC GGGTCGGCCT GGAAGAATGG GTGAGCAGGG CCTGATGGGA 
TTTATCGGTC TGGTCGGGGA GCCAGGAATC GTTGGAGAGA AGGGTAGCCG AGGTGCTATG 
GGACCACCAG GTGCTCCTGG AGCCAAGGGG TCGATGGGTC ATCCTGGTTC AGCAGGTGGA 
GTTGGAGATC CTGGAGACCC CGGGCCCCCA GGACCTCCTG GACCTCGTGG GCCACTGGGG 
ATGCGAGGAA CAAAAGGACG TCGGGGGCCC CGAGGACCAG ATGGACCAGC AGGGGAGCCA 
GGGTCAAAAG GTGTCAAGGG CCCTCCAGGA ACACAGGGCA AACAGGGCCT GGATGGACAG 
CAGGGCCAGG AAGGCGAGAG AGGGGAGCTG GGGCCTCGTG GCTTCCCGGG AATCCCGGGT 
CCTTCCGGCC CCCCGGGGGC TAAGGGCGTT CCAGGAGAAG CAGGCCCTCA AGGACCACAG 
GGACTGATTG GCCCATTGGG AGAGATTGGA CCTAAGGGAC CCCCTGGTGC TTTGGGAGAG 
CCTGGCCTTC CAGGTGAAGC TGGGATGAAG GGCGATTTTG GACCTTTGGG CATCCCTGGA 
GAGCAAGGTC TGGTTGGCCA GCGGGGAGAG ACAGGGCTGG AAGGAGATAG TGGCCCAGCT 
GGACCTGATG GTTTGAAGGG AGACAAGGGT GAGCCAGGCC CAGATGGCGA GCGAGGAGAG 
AAAGGCAAAG AAGGTCTGAA GGGCAAAGAA GGTCCCCCTG GCTATCCTGG GATCACAGGT 
GTCCGGGGTC CAGAAGGAAA ACCAGGCAAA CAAGGCGAGA AGGGACGAAC CGGACCTAAG 
GGTGCCAAGG GCTACCAAGG GCATCTTGGT GAGATGGGTG TTCCAGGAGA GGCCGGGCCA 
GCTGGCAACC AAGGCCCAAA AGGATCCCGA GGGACCCTGG GACCAACGGG TGCTCCAGGA 
AGAATGGGAG CTCAAGGAGA ACCTGGATTG CCTGGTTATG ATGGACATAA AGGACTATCA 
GGACCCATTG GACCTCCCGG GCCAAAAGGC GAGAAGGGAG AACAGGGTGA TGATGGGAAG 
ATTGAGGGCC CTCCAGGGCC TCCTGGAGAT CGGGGTCCAG TGGGTGATCG TGGAGACCGA 
GGGGAGCCTG GAGATCCTGG ATACCCTGGC CAGGAGGGTT TTGAAGGAAG CAGAGGAAAA 
GCTGGTCAGC AAGGCTTACC GGGACATCCT GGACCCCGGG GACGACCAGG ACCCAAAGGA 
TCAAAGGGTG AAGAGGGTCA GAAAGGGAAA CAAGGAAAGC GTGGAGAAGC TGGCAGTCGA 
GGAATGCAGG GCCTGGTGGG GCAGCCAGGG CCTCGAGGTG TGGTAGGAAG ACAAGGCCAA 
GAAGGACCCC CAGGTCAGGA TGGAATTCCT GGGAAGGATG GTCGAGCTGG ACATCAGGGG 
GAACAAGGAG ATGATGGAGA AGTTGGACCC ATTGGCCCTG CAGGAAGGAG AGGAAATCCA 
GGTGTGGCTG GTTTGCCTGG AGCTCAGGGA CCCCCAGGAT TCAAGGGTGA AAGTGGGTTG 
CCTGGACAAC TGGGCCCTAC TGGCAAGCGA GGGACAGAGG GAGGAACAGG GTTCCCTGGG 
AGCCAAGGGG AGCCAGGATC CAAAGGCCAG CCGGGTGACT TTGGAGAAAT GGGCTTTCCT 
GGGATTGCTG GCCTCTTTGG ACCCAAGGGC CCTCCAGGAG ATATTGGTTT CAAAGGAATC 
CAGGGGCCCC GGGGACCACC AGGTTTAATG GGAAAAGAAG GTGTCATTGG CCCCTTGGGG 
ATCCTAGGAC CGTCTGGAAT TCCGGGTCCA AAAGGGAACA AAGGAAGCCG AGGAGATTTG 
GGATTGCAAG GCCCAAGGGG TCCTCCTGGC CCCAGAGGAC GTCCTGGCCC ACCGGGCCCT 
CCTGGGATCC CCATCTCATT TCAGCAAGAT GACCTCGGAG CAGCTTTTCA GACGTGGATG 
AACTCCAACA GTGCACTTAG GACAGAGGGC TACTCCCATT CAGACCTCCT AGTGTTGGAC 
CAGGGTGGGG AGATCTTTAA GACCTTACAC TACCTCAGCA GCCTCATCCA GAGCATCAAG 
AAGCCTCTAG GAACCAAGGA GACCCCAGCC CGTATCTGTC GGGACCTTAT GAACTGTGAG 
CAGAAGATGG CCGATGGTAC ATATTGGATC GACCCAAATC TTGGCTGTGC TTCAGACACC 
ATCGAAGTCA CCTGCAATTT TACTAAAGGT GGACAGACAT GTCTCAGGCC CATCACTGCT 
TCAAAGAGCT CATTACTCTC GGGTGCTTCC AGGTGGACTT TGCCATTGGC CGGGTCCAGA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL27A1 ( XM_031954623.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031810483.1 Sarcophilus harrisii collagen type XXVII alpha 1 chain (COL27A1), transcript variant X2, mRNA.

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CloneID	OSa226381
Clone ID Related Accession (Same CDS sequence)	XM_031954623.1
Accession Version	XM_031954623.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5712bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	collagen alpha-1(XXVII) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045427.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTTAG GGGCCGGAAG GAGAGACTCC TCAACCTCTC GCCTTCGCAC GAAGGGATTC 
CTCCTAGCCT GGATTGCCTT CTGCTCTACC TGTGGCCTGG CCATCACCCA AGGAGATCCT 
GAAGATGTGG ATGTCCTGCA ACGGCTGGGC CTCGGGGGGA AGAAGTTGCC CGGCGGCCGG 
GCCCCAGGGC TGGCCCCCAT CCCCCCAGGA GTCATCCCGT TCCAGTCGGG GTTCATCTTC 
ACCCAGCGGG CCCGGATTGA AGCCCTTACA GGCGCCATCA TTCCCACCAC CTTCGGCACT 
GACCTGACCC TGGTGCTGAG CCTGTGCTCC CACCGAGTCA ACAACGCCTT CCTCTTTGCT 
GTCCGGAGCC GCAGGAAGAA GCTGCAGCTG GGGCTCCAGT TCATCCCTGG CAAGATCGTG 
CTGTTCCTGG GCCAGCGGCG CTCCCTGGCC TTCAGCTACG ACGTGCATGA CGGGCGATGG 
CACCACTTGG CCATCAGTCT CCGAGGCCAG GGGGCCACCC TCCTCACCTC CTGCGGGAAG 
CACCGCGTGC CCATGGATCT GCATTTTAAG AAAGAGGACT CTCTTGATCC CGAGGGGTCT 
TTCCTCTTTG GGAAGATGAA TCAGCACTCA GTCCAATTTG AGGGCGCCGT ATGCCAGTTC 
AATATTTATC CTTCTGCCAA GGCAGCTCAC AATTATTGTA AATATCTGAG GAAGCAGTGC 
AGACAGGCCG ACACCTACCG GCCACTGCTG CCCCCTCTTC TTCCCCTCCT TCCCCAGGAC 
TCCGAGGCCC ATACCCATCT TGGCCTCCAG CCCAGCACCT CTCTGCAGAG CCTGAGGAAT 
CTGACCACTG CGATGCCTTC GCTGGCACCC CTTAGAATGG CCAAGACCAC CCCGGCCTTG 
CCCAGAGCCC CCGGCCAAGG TGCCAGGGCT ACATGGGTGC CCTCAGCACC GGCCGGGCTT 
GGGGCACCCC CCCACCCGCC TGTGAGGACT AGTGCCGCGG ATCCCCGGGC GTCCCGATCC 
CCCACAGTGG GTACTTTGGT CTGGACCCTA CCAGCAACTG CAAAGCCATT GCCTTTCTCT 
TCAGGCAAAG CTTCCTCACC TGGGCTCAGG CCTGCACTGA TCACCCCTCA GAGCAACTTG 
ACCAAATCTC CCGTTCGCGC CATGAAGCCC ATGCCCCCAT CCAAGGCCAG TGTGGTTAAA 
ACTCCCCTTG TTCCCAGTAA GCCAGTAACT CCTTCAGTCA CTGTGGTCCC AGCTAAGAGG 
CCCCATCCCC TGGTCAAGCC GGTGCTGACC TCCCCTGCTA AGAAGCCAGT GCCCTCTGGC 
TCTCAGCCTG GCTCTGCCAA AGCCCCCCTT CCTCCCACAG CGAAACCAGC CCGTCAGAAC 
CCTGGAACAG CCAAAGCTCC TCCTTCCACT GCCAAGCCAG TGCTTCCCGC CGGGCGGTCC 
TCCCTTCCCG TTACCCCCAT GACAGCGTCC CAGGCTGCTC GGGCCAACGT GGCCAAAAAC 
CTCCCCTTTA CCAGCAAGCC GGCCAAGCTG ACCCACCCTT CCATTAAGTC ATCCTTGCCA 
GCCTCAGGTC CGAGCCTAAC CCAGACCCCT CGGCCCCCAG CAAAGCCGGT GCTTCCTACC 
GCGGGGATCA AATTGTCCAG AACAATCCCT CCCACCTTCT CCCCAGGCTC TCCTCCTGGG 
CTCAACTCCA CTGGTGACAG AAAAGTGGTT GAGCCCGCTG TGAAGAAGGA ATTGACTCCA 
AAGAGCACCC CCCAGCCACC CAGCCATCCC AGCCCTAGGA GGACTGGAGC AGTCCCTAGT 
ACCGTCTCCT TCCCTTCAGG CCTGAAGAAC CTGACCACGT GGCCCAGCCC CAAACAGGCT 
CAGCTCAGCC GTACAGCTGC CCCGGTTCTC CCACAGCACC CAGCCTCCAG CTCCCGTTTT 
GTGGGCGCCG TCACTCTCAC TCACACAGAT GGATACCTGT TCTTTGATCC AATTGGGCCC 
ACCTTGTTCC CACTGCTGTT GGGCCCTCCA GGGCAGAAGG GAGACCCTGG CCTCCCGGGC 
CCCCCTGGCT TGCCTGGGTT GCCTGGAATA TCAGGCAGAA GAGGACCCCG GGGTCCCCCT 
GGGCCTCATG GAAACCCTGG GCCTCCTGGC TCTCCTGGTG CAAAGGGACA AAAAGGAGAT 
CCTGGGCTTT CCCCTGGAAA GGCCTATGAT GGTGACAAGG GAGACGTAGG CTTACCAGGA 
CTGACTGGGA TTCCGGGACC AACTGGCCGG AAGGGGCTGA AAGGCTATCC TGGACCACCT 
GGGCATCCTG GAGACCAGGG AGAGCCAGGA CCAGAAGGGA GTCTGGGAGC CAAAGGTTAT 
CCTGGCAGGC AGGGATTACC TGGGCCAATA GGAGAACCTG GACCCAAAGG CAGTCGGGGG 
TATATTGGTC TCCCCGGGCT CTTTGGCCTG CCAGGACTTG ATGGAGAAAG GGGGCCTCCT 
GGAGCTCATG GCAAGAGGGG CAAGATGGGT AGGCCGGGAT TTCCTGGTGA CTTTGGGGAA 
AGAGGGCCCC CCGGATTGGA TGGCAACCCC GGAGAGCTGG GTGCCCCAGG CCCACCTGGA 
GTTCATGGAC TTATCGGTGA CATGGGAGCT ATTGGTCCAA TTGGCTACCC AGGGCCCAAG 
GGAATGAAGG GGCTTATGGG AAACATCGGA GAACACGGAC TGAAAGGTGA TAAGGGCGAG 
CAAGGGCTGC CTGGTGTGCC TGGAGATATC GGATTCCAAG GAGATAAGGG GAGTCAGGGA 
ATATCAGGGT TACCGGGTGC TCGAGGAAAG CCAGGTCCTA CAGGCAAGGT TGGTGACAAA 
GGACCAGTTG GGTTTCCTGG GCCTCCAGGT CCTGAGGGAT TCCCAGGGGA CATAGGACCA 
CCAGGCCAGA ATGGTCCAGA AGGTGCGAAG GGCAAGCCAG GAGCCCGAGG CTTACCAGGA 
CCGAGAGGAC AGCTTGGGCA GGAGGGAGAT GAGGGACCAA CGGGACCACC AGGATCCCCT 
GGCTTAGAGG GTCAACCTGG CCGGAAAGGA TTTCCAGGAA GGCCCGGCCC TGATGGCCCC 
AAGGGTGAGC CAGGAAACTC GGGTCGGCCT GGAAGAATGG GTGAGCAGGG CCTGATGGGA 
TTTATCGGTC TGGTCGGGGA GCCAGGAATC GTTGGAGAGA AGGGTAGCCG AGGTGCTATG 
GGACCACCAG GTGCTCCTGG AGCCAAGGGG TCGATGGGTC ATCCTGGTTC AGCAGGTGGA 
GTTGGAGATC CTGGAGACCC CGGGCCCCCA GGACCTCCTG GACCTCGTGG GCCACTGGGG 
ATGCGAGGAA CAAAAGGACG TCGGGGGCCC CGAGGACCAG ATGGACCAGC AGGGGAGCCA 
GGGTCAAAAG GTGTCAAGGG CCCTCCAGGA ACACAGGGCA AACAGGGCCT GGATGGACAG 
CAGGGCCAGG AAGGCGAGAG AGGGGAGCTG GGGCCTCGTG GCTTCCCGGG AATCCCGGGT 
CCTTCCGGCC CCCCGGGGGC TAAGGGCGTT CCAGGAGAAG CAGGCCCTCA AGGACCACAG 
GGACTGATTG GCCCATTGGG AGAGATTGGA CCTAAGGGAC CCCCTGGTGC TTTGGGAGAG 
CCTGGCCTTC CAGGTGAAGC TGGGATGAAG GGCGATTTTG GACCTTTGGG CATCCCTGGA 
GAGCAAGGTC TGGTTGGCCA GCGGGGAGAG ACAGGGCTGG AAGGAGATAG TGGCCCAGCT 
GGACCTGATG GTTTGAAGGG AGACAAGGGT GAGCCAGGCC CAGATGGCGA GCGAGGAGAG 
AAAGGCAAAG AAGGTCTGAA GGGCAAAGAA GGTCCCCCTG GCTATCCTGG GATCACAGGT 
GTCCGGGGTC CAGAAGGAAA ACCAGGCAAA CAAGGCGAGA AGGGACGAAC CGGACCTAAG 
GGTGCCAAGG GCTACCAAGG GCATCTTGGT GAGATGGGTG TTCCAGGAGA GGCCGGGCCA 
GCTGGCAACC AAGGCCCAAA AGGATCCCGA GGGACCCTGG GACCAACGGG TGCTCCAGGA 
AGAATGGGAG CTCAAGGAGA ACCTGGATTG CCTGGTTATG ATGGACATAA AGGACTATCA 
GGACCCATTG GACCTCCCGG GCCAAAAGGC GAGAAGGGAG AACAGGGTGA TGATGGGAAG 
ATTGAGGGCC CTCCAGGGCC TCCTGGAGAT CGGGGTCCAG TGGGTGATCG TGGAGACCGA 
GGGGAGCCTG GAGATCCTGG ATACCCTGGC CAGGAGGGTT TTGAAGGAAG CAGAGGAAAA 
GCTGGTCAGC AAGGCTTACC GGGACATCCT GGACCCCGGG GACGACCAGG ACCCAAAGGA 
TCAAAGGGTG AAGAGGGTCA GAAAGGGAAA CAAGGAAAGC GTGGAGAAGC TGGCAGTCGA 
GGAATGCAGG GCCTGGTGGG GCAGCCAGGG CCTCGAGGTG TGGTAGGAAG ACAAGGCCAA 
GAAGGACCCC CAGGTCAGGA TGGAATTCCT GGGAAGGATG GTCGAGCTGG ACATCAGGGG 
GAACAAGGAG ATGATGGAGA AGTTGGACCC ATTGGCCCTG CAGGAAGGAG AGGAAATCCA 
GGTGTGGCTG GTTTGCCTGG AGCTCAGGGA CCCCCAGGAT TCAAGGGTGA AAGTGGGTTG 
CCTGGACAAC TGGGCCCTAC TGGCAAGCGA GGGACAGAGG GAGGAACAGG GTTCCCTGGG 
AGCCAAGGGG AGCCAGGATC CAAAGGCCAG CCGGGTGACT TTGGAGAAAT GGGCTTTCCT 
GGGATTGCTG GCCTCTTTGG ACCCAAGGGC CCTCCAGGAG ATATTGGTTT CAAAGGAATC 
CAGGGGCCCC GGGGACCACC AGGTTTAATG GGAAAAGAAG GTGTCATTGG CCCCTTGGGG 
ATCCTAGGAC CGTCTGGAAT TCCGGGTCCA AAAGGGAACA AAGGAAGCCG AGGAGATTTG 
GGATTGCAAG GCCCAAGGGG TCCTCCTGGC CCCAGAGGAC GTCCTGGCCC ACCGGGCCCT 
CCTGGGATCC CCATCTCATT TCAGCAAGAT GACCTCGGAG CAGCTTTTCA GACGTGGATG 
AACTCCAACA GTGCACTTAG GACAGAGGGC TACTCCCATT CAGACCTCCT AGTGTTGGAC 
CAGGGTGGGG AGATCTTTAA GACCTTACAC TACCTCAGCA GCCTCATCCA GAGCATCAAG 
AAGCCTCTAG GAACCAAGGA GACCCCAGCC CGTATCTGTC GGGACCTTAT GAACTGTGAG 
CAGAAGATGG CCGATGGTAC ATATTGGATC GACCCAAATC TTGGCTGTGC TTCAGACACC 
ATCGAAGTCA CCTGCAATTT TACTAAAGGT GGACAGACAT GTCTCAGGCC CATCACTGCT 
TCAAAGGTGG ACTTTGCCAT TGGCCGGGTC CAGATGAATT TTCTTCACCT GCTGAGCTCG 
GAGGTGATCC AGCACATCAC TATCCACTGC CTCAACATGT CTGTATGGCA GGAGGGGACA 
GGCAAGGTGT CCCCCAAACA AGCCGTGCGC TTCCGAGCCT GGAATGGGCA GATCTTTGAA 
GCGGGCGGCC AGTTTGAGCC AGAGGTGTCC TCAGATGATT GCAAGGTTCA GGATGGCCGC 
TGGCACCAGA CCCTGTTCAT TTTCCGGACC CAAGACCCTC ACCAGCTGCC CATCGTCAGC 
GTCTACAACC TCCCTCCAGC CACCCCTGGG AGGCAATACC GCCTGGAAGT TGGACCTGCG 
TGCTTCCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031810483.1
CDS	200..5911
Translation	MNLGAGRRDSSTSRLRTKGFLLAWIAFCSTCGLAITQGDPEDVDVLQRLGLGGKKLPGGRAPGLAPIPPGVIPFQSGFIFTQRARIEALTGAIIPTTFGTDLTLVLSLCSHRVNNAFLFAVRSRRKKLQLGLQFIPGKIVLFLGQRRSLAFSYDVHDGRWHHLAISLRGQGATLLTSCGKHRVPMDLHFKKEDSLDPEGSFLFGKMNQHSVQFEGAVCQFNIYPSAKAAHNYCKYLRKQCRQADTYRPLLPPLLPLLPQDSEAHTHLGLQPSTSLQSLRNLTTAMPSLAPLRMAKTTPALPRAPGQGARATWVPSAPAGLGAPPHPPVRTSAADPRASRSPTVGTLVWTLPATAKPLPFSSGKASSPGLRPALITPQSNLTKSPVRAMKPMPPSKASVVKTPLVPSKPVTPSVTVVPAKRPHPLVKPVLTSPAKKPVPSGSQPGSAKAPLPPTAKPARQNPGTAKAPPSTAKPVLPAGRSSLPVTPMTASQAARANVAKNLPFTSKPAKLTHPSIKSSLPASGPSLTQTPRPPAKPVLPTAGIKLSRTIPPTFSPGSPPGLNSTGDRKVVEPAVKKELTPKSTPQPPSHPSPRRTGAVPSTVSFPSGLKNLTTWPSPKQAQLSRTAAPVLPQHPASSSRFVGAVTLTHTDGYLFFDPIGPTLFPLLLGPPGQKGDPGLPGPPGLPGLPGISGRRGPRGPPGPHGNPGPPGSPGAKGQKGDPGLSPGKAYDGDKGDVGLPGLTGIPGPTGRKGLKGYPGPPGHPGDQGEPGPEGSLGAKGYPGRQGLPGPIGEPGPKGSRGYIGLPGLFGLPGLDGERGPPGAHGKRGKMGRPGFPGDFGERGPPGLDGNPGELGAPGPPGVHGLIGDMGAIGPIGYPGPKGMKGLMGNIGEHGLKGDKGEQGLPGVPGDIGFQGDKGSQGISGLPGARGKPGPTGKVGDKGPVGFPGPPGPEGFPGDIGPPGQNGPEGAKGKPGARGLPGPRGQLGQEGDEGPTGPPGSPGLEGQPGRKGFPGRPGPDGPKGEPGNSGRPGRMGEQGLMGFIGLVGEPGIVGEKGSRGAMGPPGAPGAKGSMGHPGSAGGVGDPGDPGPPGPPGPRGPLGMRGTKGRRGPRGPDGPAGEPGSKGVKGPPGTQGKQGLDGQQGQEGERGELGPRGFPGIPGPSGPPGAKGVPGEAGPQGPQGLIGPLGEIGPKGPPGALGEPGLPGEAGMKGDFGPLGIPGEQGLVGQRGETGLEGDSGPAGPDGLKGDKGEPGPDGERGEKGKEGLKGKEGPPGYPGITGVRGPEGKPGKQGEKGRTGPKGAKGYQGHLGEMGVPGEAGPAGNQGPKGSRGTLGPTGAPGRMGAQGEPGLPGYDGHKGLSGPIGPPGPKGEKGEQGDDGKIEGPPGPPGDRGPVGDRGDRGEPGDPGYPGQEGFEGSRGKAGQQGLPGHPGPRGRPGPKGSKGEEGQKGKQGKRGEAGSRGMQGLVGQPGPRGVVGRQGQEGPPGQDGIPGKDGRAGHQGEQGDDGEVGPIGPAGRRGNPGVAGLPGAQGPPGFKGESGLPGQLGPTGKRGTEGGTGFPGSQGEPGSKGQPGDFGEMGFPGIAGLFGPKGPPGDIGFKGIQGPRGPPGLMGKEGVIGPLGILGPSGIPGPKGNKGSRGDLGLQGPRGPPGPRGRPGPPGPPGIPISFQQDDLGAAFQTWMNSNSALRTEGYSHSDLLVLDQGGEIFKTLHYLSSLIQSIKKPLGTKETPARICRDLMNCEQKMADGTYWIDPNLGCASDTIEVTCNFTKGGQTCLRPITASKVDFAIGRVQMNFLHLLSSEVIQHITIHCLNMSVWQEGTGKVSPKQAVRFRAWNGQIFEAGGQFEPEVSSDDCKVQDGRWHQTLFIFRTQDPHQLPIVSVYNLPPATPGRQYRLEVGPACFL

Target ORF information:

RefSeq Version	XM_031954623.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii collagen type XXVII alpha 1 chain (COL27A1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031954623.1

ORF Insert Sequence:

ATGAATTTAG GGGCCGGAAG GAGAGACTCC TCAACCTCTC GCCTTCGCAC GAAGGGATTC 
CTCCTAGCCT GGATTGCCTT CTGCTCTACC TGTGGCCTGG CCATCACCCA AGGAGATCCT 
GAAGATGTGG ATGTCCTGCA ACGGCTGGGC CTCGGGGGGA AGAAGTTGCC CGGCGGCCGG 
GCCCCAGGGC TGGCCCCCAT CCCCCCAGGA GTCATCCCGT TCCAGTCGGG GTTCATCTTC 
ACCCAGCGGG CCCGGATTGA AGCCCTTACA GGCGCCATCA TTCCCACCAC CTTCGGCACT 
GACCTGACCC TGGTGCTGAG CCTGTGCTCC CACCGAGTCA ACAACGCCTT CCTCTTTGCT 
GTCCGGAGCC GCAGGAAGAA GCTGCAGCTG GGGCTCCAGT TCATCCCTGG CAAGATCGTG 
CTGTTCCTGG GCCAGCGGCG CTCCCTGGCC TTCAGCTACG ACGTGCATGA CGGGCGATGG 
CACCACTTGG CCATCAGTCT CCGAGGCCAG GGGGCCACCC TCCTCACCTC CTGCGGGAAG 
CACCGCGTGC CCATGGATCT GCATTTTAAG AAAGAGGACT CTCTTGATCC CGAGGGGTCT 
TTCCTCTTTG GGAAGATGAA TCAGCACTCA GTCCAATTTG AGGGCGCCGT ATGCCAGTTC 
AATATTTATC CTTCTGCCAA GGCAGCTCAC AATTATTGTA AATATCTGAG GAAGCAGTGC 
AGACAGGCCG ACACCTACCG GCCACTGCTG CCCCCTCTTC TTCCCCTCCT TCCCCAGGAC 
TCCGAGGCCC ATACCCATCT TGGCCTCCAG CCCAGCACCT CTCTGCAGAG CCTGAGGAAT 
CTGACCACTG CGATGCCTTC GCTGGCACCC CTTAGAATGG CCAAGACCAC CCCGGCCTTG 
CCCAGAGCCC CCGGCCAAGG TGCCAGGGCT ACATGGGTGC CCTCAGCACC GGCCGGGCTT 
GGGGCACCCC CCCACCCGCC TGTGAGGACT AGTGCCGCGG ATCCCCGGGC GTCCCGATCC 
CCCACAGTGG GTACTTTGGT CTGGACCCTA CCAGCAACTG CAAAGCCATT GCCTTTCTCT 
TCAGGCAAAG CTTCCTCACC TGGGCTCAGG CCTGCACTGA TCACCCCTCA GAGCAACTTG 
ACCAAATCTC CCGTTCGCGC CATGAAGCCC ATGCCCCCAT CCAAGGCCAG TGTGGTTAAA 
ACTCCCCTTG TTCCCAGTAA GCCAGTAACT CCTTCAGTCA CTGTGGTCCC AGCTAAGAGG 
CCCCATCCCC TGGTCAAGCC GGTGCTGACC TCCCCTGCTA AGAAGCCAGT GCCCTCTGGC 
TCTCAGCCTG GCTCTGCCAA AGCCCCCCTT CCTCCCACAG CGAAACCAGC CCGTCAGAAC 
CCTGGAACAG CCAAAGCTCC TCCTTCCACT GCCAAGCCAG TGCTTCCCGC CGGGCGGTCC 
TCCCTTCCCG TTACCCCCAT GACAGCGTCC CAGGCTGCTC GGGCCAACGT GGCCAAAAAC 
CTCCCCTTTA CCAGCAAGCC GGCCAAGCTG ACCCACCCTT CCATTAAGTC ATCCTTGCCA 
GCCTCAGGTC CGAGCCTAAC CCAGACCCCT CGGCCCCCAG CAAAGCCGGT GCTTCCTACC 
GCGGGGATCA AATTGTCCAG AACAATCCCT CCCACCTTCT CCCCAGGCTC TCCTCCTGGG 
CTCAACTCCA CTGGTGACAG AAAAGTGGTT GAGCCCGCTG TGAAGAAGGA ATTGACTCCA 
AAGAGCACCC CCCAGCCACC CAGCCATCCC AGCCCTAGGA GGACTGGAGC AGTCCCTAGT 
ACCGTCTCCT TCCCTTCAGG CCTGAAGAAC CTGACCACGT GGCCCAGCCC CAAACAGGCT 
CAGCTCAGCC GTACAGCTGC CCCGGTTCTC CCACAGCACC CAGCCTCCAG CTCCCGTTTT 
GTGGGCGCCG TCACTCTCAC TCACACAGAT GGATACCTGT TCTTTGATCC AATTGGGCCC 
ACCTTGTTCC CACTGCTGTT GGGCCCTCCA GGGCAGAAGG GAGACCCTGG CCTCCCGGGC 
CCCCCTGGCT TGCCTGGGTT GCCTGGAATA TCAGGCAGAA GAGGACCCCG GGGTCCCCCT 
GGGCCTCATG GAAACCCTGG GCCTCCTGGC TCTCCTGGTG CAAAGGGACA AAAAGGAGAT 
CCTGGGCTTT CCCCTGGAAA GGCCTATGAT GGTGACAAGG GAGACGTAGG CTTACCAGGA 
CTGACTGGGA TTCCGGGACC AACTGGCCGG AAGGGGCTGA AAGGCTATCC TGGACCACCT 
GGGCATCCTG GAGACCAGGG AGAGCCAGGA CCAGAAGGGA GTCTGGGAGC CAAAGGTTAT 
CCTGGCAGGC AGGGATTACC TGGGCCAATA GGAGAACCTG GACCCAAAGG CAGTCGGGGG 
TATATTGGTC TCCCCGGGCT CTTTGGCCTG CCAGGACTTG ATGGAGAAAG GGGGCCTCCT 
GGAGCTCATG GCAAGAGGGG CAAGATGGGT AGGCCGGGAT TTCCTGGTGA CTTTGGGGAA 
AGAGGGCCCC CCGGATTGGA TGGCAACCCC GGAGAGCTGG GTGCCCCAGG CCCACCTGGA 
GTTCATGGAC TTATCGGTGA CATGGGAGCT ATTGGTCCAA TTGGCTACCC AGGGCCCAAG 
GGAATGAAGG GGCTTATGGG AAACATCGGA GAACACGGAC TGAAAGGTGA TAAGGGCGAG 
CAAGGGCTGC CTGGTGTGCC TGGAGATATC GGATTCCAAG GAGATAAGGG GAGTCAGGGA 
ATATCAGGGT TACCGGGTGC TCGAGGAAAG CCAGGTCCTA CAGGCAAGGT TGGTGACAAA 
GGACCAGTTG GGTTTCCTGG GCCTCCAGGT CCTGAGGGAT TCCCAGGGGA CATAGGACCA 
CCAGGCCAGA ATGGTCCAGA AGGTGCGAAG GGCAAGCCAG GAGCCCGAGG CTTACCAGGA 
CCGAGAGGAC AGCTTGGGCA GGAGGGAGAT GAGGGACCAA CGGGACCACC AGGATCCCCT 
GGCTTAGAGG GTCAACCTGG CCGGAAAGGA TTTCCAGGAA GGCCCGGCCC TGATGGCCCC 
AAGGGTGAGC CAGGAAACTC GGGTCGGCCT GGAAGAATGG GTGAGCAGGG CCTGATGGGA 
TTTATCGGTC TGGTCGGGGA GCCAGGAATC GTTGGAGAGA AGGGTAGCCG AGGTGCTATG 
GGACCACCAG GTGCTCCTGG AGCCAAGGGG TCGATGGGTC ATCCTGGTTC AGCAGGTGGA 
GTTGGAGATC CTGGAGACCC CGGGCCCCCA GGACCTCCTG GACCTCGTGG GCCACTGGGG 
ATGCGAGGAA CAAAAGGACG TCGGGGGCCC CGAGGACCAG ATGGACCAGC AGGGGAGCCA 
GGGTCAAAAG GTGTCAAGGG CCCTCCAGGA ACACAGGGCA AACAGGGCCT GGATGGACAG 
CAGGGCCAGG AAGGCGAGAG AGGGGAGCTG GGGCCTCGTG GCTTCCCGGG AATCCCGGGT 
CCTTCCGGCC CCCCGGGGGC TAAGGGCGTT CCAGGAGAAG CAGGCCCTCA AGGACCACAG 
GGACTGATTG GCCCATTGGG AGAGATTGGA CCTAAGGGAC CCCCTGGTGC TTTGGGAGAG 
CCTGGCCTTC CAGGTGAAGC TGGGATGAAG GGCGATTTTG GACCTTTGGG CATCCCTGGA 
GAGCAAGGTC TGGTTGGCCA GCGGGGAGAG ACAGGGCTGG AAGGAGATAG TGGCCCAGCT 
GGACCTGATG GTTTGAAGGG AGACAAGGGT GAGCCAGGCC CAGATGGCGA GCGAGGAGAG 
AAAGGCAAAG AAGGTCTGAA GGGCAAAGAA GGTCCCCCTG GCTATCCTGG GATCACAGGT 
GTCCGGGGTC CAGAAGGAAA ACCAGGCAAA CAAGGCGAGA AGGGACGAAC CGGACCTAAG 
GGTGCCAAGG GCTACCAAGG GCATCTTGGT GAGATGGGTG TTCCAGGAGA GGCCGGGCCA 
GCTGGCAACC AAGGCCCAAA AGGATCCCGA GGGACCCTGG GACCAACGGG TGCTCCAGGA 
AGAATGGGAG CTCAAGGAGA ACCTGGATTG CCTGGTTATG ATGGACATAA AGGACTATCA 
GGACCCATTG GACCTCCCGG GCCAAAAGGC GAGAAGGGAG AACAGGGTGA TGATGGGAAG 
ATTGAGGGCC CTCCAGGGCC TCCTGGAGAT CGGGGTCCAG TGGGTGATCG TGGAGACCGA 
GGGGAGCCTG GAGATCCTGG ATACCCTGGC CAGGAGGGTT TTGAAGGAAG CAGAGGAAAA 
GCTGGTCAGC AAGGCTTACC GGGACATCCT GGACCCCGGG GACGACCAGG ACCCAAAGGA 
TCAAAGGGTG AAGAGGGTCA GAAAGGGAAA CAAGGAAAGC GTGGAGAAGC TGGCAGTCGA 
GGAATGCAGG GCCTGGTGGG GCAGCCAGGG CCTCGAGGTG TGGTAGGAAG ACAAGGCCAA 
GAAGGACCCC CAGGTCAGGA TGGAATTCCT GGGAAGGATG GTCGAGCTGG ACATCAGGGG 
GAACAAGGAG ATGATGGAGA AGTTGGACCC ATTGGCCCTG CAGGAAGGAG AGGAAATCCA 
GGTGTGGCTG GTTTGCCTGG AGCTCAGGGA CCCCCAGGAT TCAAGGGTGA AAGTGGGTTG 
CCTGGACAAC TGGGCCCTAC TGGCAAGCGA GGGACAGAGG GAGGAACAGG GTTCCCTGGG 
AGCCAAGGGG AGCCAGGATC CAAAGGCCAG CCGGGTGACT TTGGAGAAAT GGGCTTTCCT 
GGGATTGCTG GCCTCTTTGG ACCCAAGGGC CCTCCAGGAG ATATTGGTTT CAAAGGAATC 
CAGGGGCCCC GGGGACCACC AGGTTTAATG GGAAAAGAAG GTGTCATTGG CCCCTTGGGG 
ATCCTAGGAC CGTCTGGAAT TCCGGGTCCA AAAGGGAACA AAGGAAGCCG AGGAGATTTG 
GGATTGCAAG GCCCAAGGGG TCCTCCTGGC CCCAGAGGAC GTCCTGGCCC ACCGGGCCCT 
CCTGGGATCC CCATCTCATT TCAGCAAGAT GACCTCGGAG CAGCTTTTCA GACGTGGATG 
AACTCCAACA GTGCACTTAG GACAGAGGGC TACTCCCATT CAGACCTCCT AGTGTTGGAC 
CAGGGTGGGG AGATCTTTAA GACCTTACAC TACCTCAGCA GCCTCATCCA GAGCATCAAG 
AAGCCTCTAG GAACCAAGGA GACCCCAGCC CGTATCTGTC GGGACCTTAT GAACTGTGAG 
CAGAAGATGG CCGATGGTAC ATATTGGATC GACCCAAATC TTGGCTGTGC TTCAGACACC 
ATCGAAGTCA CCTGCAATTT TACTAAAGGT GGACAGACAT GTCTCAGGCC CATCACTGCT 
TCAAAGGTGG ACTTTGCCAT TGGCCGGGTC CAGATGAATT TTCTTCACCT GCTGAGCTCG 
GAGGTGATCC AGCACATCAC TATCCACTGC CTCAACATGT CTGTATGGCA GGAGGGGACA 
GGCAAGGTGT CCCCCAAACA AGCCGTGCGC TTCCGAGCCT GGAATGGGCA GATCTTTGAA 
GCGGGCGGCC AGTTTGAGCC AGAGGTGTCC TCAGATGATT GCAAGGTTCA GGATGGCCGC 
TGGCACCAGA CCCTGTTCAT TTTCCGGACC CAAGACCCTC ACCAGCTGCC CATCGTCAGC 
GTCTACAACC TCCCTCCAGC CACCCCTGGG AGGCAATACC GCCTGGAAGT TGGACCTGCG 
TGCTTCCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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1-877-436-7274

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COL27A1 cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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