RAPGEF1 gene cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

mRNA	Protein	Name
XM_012553967.2	XP_012409421.2	rap guanine nucleotide exchange factor 1 isoform X1
XM_012553968.2	XP_012409422.2	rap guanine nucleotide exchange factor 1 isoform X2
XM_031954874.1	XP_031810734.1	rap guanine nucleotide exchange factor 1 isoform X1
XM_012553967.3	XP_012409421.3	rap guanine nucleotide exchange factor 1 isoform X5
XM_012553968.1	XP_012409422.1	rap guanine nucleotide exchange factor 1 isoform X2
XM_031954875.1	XP_031810735.1	rap guanine nucleotide exchange factor 1 isoform X2
XM_031954877.1	XP_031810737.1	rap guanine nucleotide exchange factor 1 isoform X4
XM_031954876.1	XP_031810736.1	rap guanine nucleotide exchange factor 1 isoform X3
XM_012553967.1	XP_012409421.1	rap guanine nucleotide exchange factor 1 isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSa119027	XM_012553967.1 Latest version!	Sarcophilus harrisii Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OSa119028	XM_012553968.1 Latest version!	Sarcophilus harrisii Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OSa150375	XM_012553967.2 Latest version!	Sarcophilus harrisii Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OSa150376	XM_012553968.2 Latest version!	Sarcophilus harrisii Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OSa226538	XM_031954874.1 Latest version!	Sarcophilus harrisii Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OSa226539	XM_031954875.1 Latest version!	Sarcophilus harrisii Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OSa226540	XM_031954876.1 Latest version!	Sarcophilus harrisii Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OSa226541	XM_031954877.1 Latest version!	Sarcophilus harrisii Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OSa226542	XM_012553967.3 Latest version!	Sarcophilus harrisii Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

RAPGEF1 ( XM_012553967.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_012409421.1 Sarcophilus harrisii Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSa119027
Clone ID Related Accession (Same CDS sequence)	XM_012553967.1
Accession Version	XM_012553967.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3177bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1431532800000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	rap guanine nucleotide exchange factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003816662.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sarcophilus harrisii Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA TGGACAAATT TCACTCGCCC AAAATCAAGA GAACACCATC AAAGAAAGGA 
AAACCAGCCG ATGTGCCTGT GAAAACTTTT GAGAAACCTG TGAACAAAGA GGCCACAGAC 
AATTTTCTAC CAGAGGGCTA CCCTCCTCCC TTGGATCTGG ATCACCAAGC AGTACAATTT 
ATGTCCACCA GTGCTGTGGT TCCCAGGTCT CAGAGGCAGA AGAATCTCAG CTGGCTGGAA 
GAGAAAGAAA AAGAGGTGGT CAGTGCCTTG CGCTACTTTA AGACCATTGT GGACAAAATG 
GCAATTGACA AGAAGGTACT GGAAATGCTT CCAGGTTCGG CCAGCAAGGT CCTGGAGGCC 
ATCCTGCCCC TAGTGCAGAC AGACCCTCGC ATTCAGCAGA GCTCAGCCAT CTCCTCTTGC 
TACAGCCGGG TGTATCAGAG TCTAGCCAAT CTCATTCGTT GGTCAGATCA AGTGATGCTG 
GAAGGTGTGA ACTCGGAAGA TAAAGAGATG GTGACAACTG TGAAAGAAGT CATCAAAGCT 
GTTTTGGATG GAGTGAAGGA GCTTGTCAGG CTCACAATAG AGAAGCAGGA GCATCCATCT 
CCCACAAGTC CAGTTAAGCC CAGCTCACCA GCCTGCAAAA CTGACAGCCA ACCTGAGCTC 
CCATTGACTG ACCGGGAAAT GGAGATTCTC AACAAGACAA CTAGTATGTC CCAATCAACG 
GAGCTGCTGC ATCCTGACTC CATGGATGAA GAGGTTGCTC CTCCCAAGCC CCCCCTCCCT 
GGCATCCGAG TGGTGGATAA TAGTCCTCCA CCAGCTTTAC CACCCAAAAA GAGGCAGTCG 
GCACCATCTC CCACCAGAGT GGCTGTGGTT GCACCCATGA GCCGGGCCAC CAGTGGCTCA 
AGTTTACCTG TTGGAATCAA TAGACAGGAC TTTGATGTGG ACTGTTATGC CCAAAGGAGG 
CTGTCTGGGG GCAGCCATTC ATATGGCGGG GAATCCCCTC GCCTCTCCCC GTGCAGCAGC 
ATCGGCAAGC TGAGCAAGTC CGACGAACAG CTGTCCTCCT TGGACCGGGA CAGTGGCCAG 
TGCTCCAGGA ATACAAGCTG TGAAACACTA GATCACTATG ACCCTGATTA TGAATTTCTA 
CAGCAAGACC TCTCCACTGC TGACCAGATA CCTCAGCAAG TAGCATGTAA CCTTAGCCCA 
TTGCCAGAAT CTGTTGGTGA ATCTGGCTCT CCTTTCCATG GACGTCCTTT CCAGCTGCCA 
CCCAGCAACT CCCCACAGCC AGAGCTTGGC AATCCCTTGC CCATGGGGCA TCCTACGGAC 
ACACCTCCTG CCCTGCCTGA GAAGAAGCGG AGGAGTGCAG CCTCTCAGAC CACGGACAGC 
TCCAGCTCCA GCTGTAGAGT CTCCTATGAG AGGCACCCTT CCCAATATGA CAATATCATG 
GAGGATGATC TCCAGAACCC GGCCTCCCTC CAGCCAGGAT CATTCACGCC ATTCGCTGCT 
GTCCTCCCCT TCCAGCAAGG AGGCTCTTCA GCCAATTTTG TGTCTGATTT TGCTGCTCCC 
GATTCAATGG GTGACCCCGA GAAACCACCT CCTCTACCTG AAAAGAAGAA CAAACACATG 
CTGGCCTACA TGCAGCTCCT TGAGGACTAC TCAGAGCCCC AGCCTTCCAT GTTCTACCAG 
ACGCCCCAGA ACGAGCACAT CTACCAGCAG AAGAACAAGC TCCTCATGGA GGTCTACGGC 
TTTAATGACT CCTTCACCCA GGAGCTGGCC CCGCCCCCTG CCCTGCCCCC CAAGCAGAGG 
CAGCTGGAAT CACCAGCTAC AAAAGATGGA CATCCCAAAG ATTCCTCTTC ACATGGTGGG 
AAAGAAAACA GAGAAGGTGG TGAAAGGCAG CTGAAGTCCC CCGATGGTTT GGAATCGACC 
CAGTCAGAGG AGGAAGTGGA TGAACTCTCT CTGATTGACC ACAATGAAAT TATGGCTAGG 
TTGACGTTAA AACAAGAGGG TGATGATGGG CCAGATGTCC GTGGGGGATC TGGGGATATA 
TTGCTGGTCC ATGCAACTGA GACCGATAGA AAAGACTTGG TTTTGTACTG TGAAGCCTTT 
TTGACAACCT ACAGGACATT TATCACCCCT GAGGAACTCA TTAAGAAGCT ACAGTATAGA 
TATGAGAAGT TCTGCCCCTT CCCAGACACA TTCAAGAAGC GAGTCAGCAA GAATACTTTC 
TTCGTGCTGG TGCGGGTAGT GGATGAACTG TGCCTGGTGG AATTAACTGA AGAGATCCTA 
AAGCTCTTGA TGGAGCTGGT GTTCCGGTTA GTGTGCAGTG GGGAGCTGAG CCTTGCCCGG 
GTCCTCCGGA AGAATATCTT GGACAAAGTG AACCAAAAGA AGATGCTAAA GTGTGCCAAC 
TCGGACCAAC CCCTGGCTGC TCGAGGGGTG GCTGCCAGGC CTGGGACTCT GCATGACTTC 
CACAGCCATG AGATTGCTGA GCAGCTGACA CTGTTGGATG CTGAGCTCTT CTACAAGATA 
GAGATCCCAG AAGTTTTGCT TTGGGCAAAA GAGCAGAATG AAGAGAAGAG CCCTAACTTG 
ACACAGTTTA CTGAGCACTT TAATAACATG TCCTACTGGG TTCGTTCAAT AATCATGTTG 
CAAGAAAAAG CCCAGGACAG AGAACGGCTG CTTCTGAAAT TTATCAAGAT CATGAAGCAC 
TTGAGGAAGC TAAATAACTT CAACTCGTAT CTGGCTATCC TTTCAGCACT GGACTCAGCA 
CCCATCCGGA GGCTGGAGTG GCAAAAACAG ACCTCAGAGG GCCTGGCCGA GTACTGCACT 
TTGATTGACA GCTCTTCCTC CTTCCGGGCC TATCGGGCGG CTCTTTCTGA GGTGGAACCT 
CCATGCATCC CATACCTGGG TCTGATCCTG CAGGACCTCA CCTTTGTTCA CCTCGGAAAC 
CCAGACTACA TTGACGGGAA AGTGAATTTT TCCAAGCGCT GGCAGCAGTT CAACATCCTG 
GACAGTATGA GATGTTTCCA GCAGGCACAT TATGAAATCC GCAGAAATGA GGACATCGTG 
AACTTCTTCA ATGATTTCAG TGACCACCTA GCCGAGGAGG CCTTATGGGA ACTCTCTCTC 
AAAATCAAAC CCCGGAACAT AACTCGGCGA AAAACAGATC GAGAAGAGAA GACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012409421.1
CDS	30..3206
Translation	MKLMDKFHSPKIKRTPSKKGKPADVPVKTFEKPVNKEATDNFLPEGYPPPLDLDHQAVQFMSTSAVVPRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQTDPRIQQSSAISSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKEVIKAVLDGVKELVRLTIEKQEHPSPTSPVKPSSPACKTDSQPELPLTDREMEILNKTTSMSQSTELLHPDSMDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSTADQIPQQVACNLSPLPESVGESGSPFHGRPFQLPPSNSPQPELGNPLPMGHPTDTPPALPEKKRRSAASQTTDSSSSSCRVSYERHPSQYDNIMEDDLQNPASLQPGSFTPFAAVLPFQQGGSSANFVSDFAAPDSMGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFNDSFTQELAPPPALPPKQRQLESPATKDGHPKDSSSHGGKENREGGERQLKSPDGLESTQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIKKLQYRYEKFCPFPDTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCSGELSLARVLRKNILDKVNQKKMLKCANSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYEIRRNEDIVNFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_012553967.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012553967.1

ORF Insert Sequence:

ATGAAGCTGA TGGACAAATT TCACTCGCCC AAAATCAAGA GAACACCATC AAAGAAAGGA 
AAACCAGCCG ATGTGCCTGT GAAAACTTTT GAGAAACCTG TGAACAAAGA GGCCACAGAC 
AATTTTCTAC CAGAGGGCTA CCCTCCTCCC TTGGATCTGG ATCACCAAGC AGTACAATTT 
ATGTCCACCA GTGCTGTGGT TCCCAGGTCT CAGAGGCAGA AGAATCTCAG CTGGCTGGAA 
GAGAAAGAAA AAGAGGTGGT CAGTGCCTTG CGCTACTTTA AGACCATTGT GGACAAAATG 
GCAATTGACA AGAAGGTACT GGAAATGCTT CCAGGTTCGG CCAGCAAGGT CCTGGAGGCC 
ATCCTGCCCC TAGTGCAGAC AGACCCTCGC ATTCAGCAGA GCTCAGCCAT CTCCTCTTGC 
TACAGCCGGG TGTATCAGAG TCTAGCCAAT CTCATTCGTT GGTCAGATCA AGTGATGCTG 
GAAGGTGTGA ACTCGGAAGA TAAAGAGATG GTGACAACTG TGAAAGAAGT CATCAAAGCT 
GTTTTGGATG GAGTGAAGGA GCTTGTCAGG CTCACAATAG AGAAGCAGGA GCATCCATCT 
CCCACAAGTC CAGTTAAGCC CAGCTCACCA GCCTGCAAAA CTGACAGCCA ACCTGAGCTC 
CCATTGACTG ACCGGGAAAT GGAGATTCTC AACAAGACAA CTAGTATGTC CCAATCAACG 
GAGCTGCTGC ATCCTGACTC CATGGATGAA GAGGTTGCTC CTCCCAAGCC CCCCCTCCCT 
GGCATCCGAG TGGTGGATAA TAGTCCTCCA CCAGCTTTAC CACCCAAAAA GAGGCAGTCG 
GCACCATCTC CCACCAGAGT GGCTGTGGTT GCACCCATGA GCCGGGCCAC CAGTGGCTCA 
AGTTTACCTG TTGGAATCAA TAGACAGGAC TTTGATGTGG ACTGTTATGC CCAAAGGAGG 
CTGTCTGGGG GCAGCCATTC ATATGGCGGG GAATCCCCTC GCCTCTCCCC GTGCAGCAGC 
ATCGGCAAGC TGAGCAAGTC CGACGAACAG CTGTCCTCCT TGGACCGGGA CAGTGGCCAG 
TGCTCCAGGA ATACAAGCTG TGAAACACTA GATCACTATG ACCCTGATTA TGAATTTCTA 
CAGCAAGACC TCTCCACTGC TGACCAGATA CCTCAGCAAG TAGCATGTAA CCTTAGCCCA 
TTGCCAGAAT CTGTTGGTGA ATCTGGCTCT CCTTTCCATG GACGTCCTTT CCAGCTGCCA 
CCCAGCAACT CCCCACAGCC AGAGCTTGGC AATCCCTTGC CCATGGGGCA TCCTACGGAC 
ACACCTCCTG CCCTGCCTGA GAAGAAGCGG AGGAGTGCAG CCTCTCAGAC CACGGACAGC 
TCCAGCTCCA GCTGTAGAGT CTCCTATGAG AGGCACCCTT CCCAATATGA CAATATCATG 
GAGGATGATC TCCAGAACCC GGCCTCCCTC CAGCCAGGAT CATTCACGCC ATTCGCTGCT 
GTCCTCCCCT TCCAGCAAGG AGGCTCTTCA GCCAATTTTG TGTCTGATTT TGCTGCTCCC 
GATTCAATGG GTGACCCCGA GAAACCACCT CCTCTACCTG AAAAGAAGAA CAAACACATG 
CTGGCCTACA TGCAGCTCCT TGAGGACTAC TCAGAGCCCC AGCCTTCCAT GTTCTACCAG 
ACGCCCCAGA ACGAGCACAT CTACCAGCAG AAGAACAAGC TCCTCATGGA GGTCTACGGC 
TTTAATGACT CCTTCACCCA GGAGCTGGCC CCGCCCCCTG CCCTGCCCCC CAAGCAGAGG 
CAGCTGGAAT CACCAGCTAC AAAAGATGGA CATCCCAAAG ATTCCTCTTC ACATGGTGGG 
AAAGAAAACA GAGAAGGTGG TGAAAGGCAG CTGAAGTCCC CCGATGGTTT GGAATCGACC 
CAGTCAGAGG AGGAAGTGGA TGAACTCTCT CTGATTGACC ACAATGAAAT TATGGCTAGG 
TTGACGTTAA AACAAGAGGG TGATGATGGG CCAGATGTCC GTGGGGGATC TGGGGATATA 
TTGCTGGTCC ATGCAACTGA GACCGATAGA AAAGACTTGG TTTTGTACTG TGAAGCCTTT 
TTGACAACCT ACAGGACATT TATCACCCCT GAGGAACTCA TTAAGAAGCT ACAGTATAGA 
TATGAGAAGT TCTGCCCCTT CCCAGACACA TTCAAGAAGC GAGTCAGCAA GAATACTTTC 
TTCGTGCTGG TGCGGGTAGT GGATGAACTG TGCCTGGTGG AATTAACTGA AGAGATCCTA 
AAGCTCTTGA TGGAGCTGGT GTTCCGGTTA GTGTGCAGTG GGGAGCTGAG CCTTGCCCGG 
GTCCTCCGGA AGAATATCTT GGACAAAGTG AACCAAAAGA AGATGCTAAA GTGTGCCAAC 
TCGGACCAAC CCCTGGCTGC TCGAGGGGTG GCTGCCAGGC CTGGGACTCT GCATGACTTC 
CACAGCCATG AGATTGCTGA GCAGCTGACA CTGTTGGATG CTGAGCTCTT CTACAAGATA 
GAGATCCCAG AAGTTTTGCT TTGGGCAAAA GAGCAGAATG AAGAGAAGAG CCCTAACTTG 
ACACAGTTTA CTGAGCACTT TAATAACATG TCCTACTGGG TTCGTTCAAT AATCATGTTG 
CAAGAAAAAG CCCAGGACAG AGAACGGCTG CTTCTGAAAT TTATCAAGAT CATGAAGCAC 
TTGAGGAAGC TAAATAACTT CAACTCGTAT CTGGCTATCC TTTCAGCACT GGACTCAGCA 
CCCATCCGGA GGCTGGAGTG GCAAAAACAG ACCTCAGAGG GCCTGGCCGA GTACTGCACT 
TTGATTGACA GCTCTTCCTC CTTCCGGGCC TATCGGGCGG CTCTTTCTGA GGTGGAACCT 
CCATGCATCC CATACCTGGG TCTGATCCTG CAGGACCTCA CCTTTGTTCA CCTCGGAAAC 
CCAGACTACA TTGACGGGAA AGTGAATTTT TCCAAGCGCT GGCAGCAGTT CAACATCCTG 
GACAGTATGA GATGTTTCCA GCAGGCACAT TATGAAATCC GCAGAAATGA GGACATCGTG 
AACTTCTTCA ATGATTTCAG TGACCACCTA GCCGAGGAGG CCTTATGGGA ACTCTCTCTC 
AAAATCAAAC CCCGGAACAT AACTCGGCGA AAAACAGATC GAGAAGAGAA GACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_012553968.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_012409422.1 Sarcophilus harrisii Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSa119028
Clone ID Related Accession (Same CDS sequence)	XM_012553968.1
Accession Version	XM_012553968.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3063bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1431532800000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	rap guanine nucleotide exchange factor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003816662.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sarcophilus harrisii Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA TGGACAAATT TCACTCGCCC AAAATCAAGA GAACACCATC AAAGAAAGGA 
AAACCAGCCG ATGTGCCTGT GAAAACTTTT GAGAAACCTG TGAACAAAAA TCTCAGCTGG 
CTGGAAGAGA AAGAAAAAGA GGTGGTCAGT GCCTTGCGCT ACTTTAAGAC CATTGTGGAC 
AAAATGGCAA TTGACAAGAA GGTACTGGAA ATGCTTCCAG GTTCGGCCAG CAAGGTCCTG 
GAGGCCATCC TGCCCCTAGT GCAGACAGAC CCTCGCATTC AGCAGAGCTC AGCCATCTCC 
TCTTGCTACA GCCGGGTGTA TCAGAGTCTA GCCAATCTCA TTCGTTGGTC AGATCAAGTG 
ATGCTGGAAG GTGTGAACTC GGAAGATAAA GAGATGGTGA CAACTGTGAA AGAAGTCATC 
AAAGCTGTTT TGGATGGAGT GAAGGAGCTT GTCAGGCTCA CAATAGAGAA GCAGGAGCAT 
CCATCTCCCA CAAGTCCAGT TAAGCCCAGC TCACCAGCCT GCAAAACTGA CAGCCAACCT 
GAGCTCCCAT TGACTGACCG GGAAATGGAG ATTCTCAACA AGACAACTAG TATGTCCCAA 
TCAACGGAGC TGCTGCATCC TGACTCCATG GATGAAGAGG TTGCTCCTCC CAAGCCCCCC 
CTCCCTGGCA TCCGAGTGGT GGATAATAGT CCTCCACCAG CTTTACCACC CAAAAAGAGG 
CAGTCGGCAC CATCTCCCAC CAGAGTGGCT GTGGTTGCAC CCATGAGCCG GGCCACCAGT 
GGCTCAAGTT TACCTGTTGG AATCAATAGA CAGGACTTTG ATGTGGACTG TTATGCCCAA 
AGGAGGCTGT CTGGGGGCAG CCATTCATAT GGCGGGGAAT CCCCTCGCCT CTCCCCGTGC 
AGCAGCATCG GCAAGCTGAG CAAGTCCGAC GAACAGCTGT CCTCCTTGGA CCGGGACAGT 
GGCCAGTGCT CCAGGAATAC AAGCTGTGAA ACACTAGATC ACTATGACCC TGATTATGAA 
TTTCTACAGC AAGACCTCTC CACTGCTGAC CAGATACCTC AGCAAGTAGC ATGTAACCTT 
AGCCCATTGC CAGAATCTGT TGGTGAATCT GGCTCTCCTT TCCATGGACG TCCTTTCCAG 
CTGCCACCCA GCAACTCCCC ACAGCCAGAG CTTGGCAATC CCTTGCCCAT GGGGCATCCT 
ACGGACACAC CTCCTGCCCT GCCTGAGAAG AAGCGGAGGA GTGCAGCCTC TCAGACCACG 
GACAGCTCCA GCTCCAGCTG TAGAGTCTCC TATGAGAGGC ACCCTTCCCA ATATGACAAT 
ATCATGGAGG ATGATCTCCA GAACCCGGCC TCCCTCCAGC CAGGATCATT CACGCCATTC 
GCTGCTGTCC TCCCCTTCCA GCAAGGAGGC TCTTCAGCCA ATTTTGTGTC TGATTTTGCT 
GCTCCCGATT CAATGGGTGA CCCCGAGAAA CCACCTCCTC TACCTGAAAA GAAGAACAAA 
CACATGCTGG CCTACATGCA GCTCCTTGAG GACTACTCAG AGCCCCAGCC TTCCATGTTC 
TACCAGACGC CCCAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCTCCT CATGGAGGTC 
TACGGCTTTA ATGACTCCTT CACCCAGGAG CTGGCCCCGC CCCCTGCCCT GCCCCCCAAG 
CAGAGGCAGC TGGAATCACC AGCTACAAAA GATGGACATC CCAAAGATTC CTCTTCACAT 
GGTGGGAAAG AAAACAGAGA AGGTGGTGAA AGGCAGCTGA AGTCCCCCGA TGGTTTGGAA 
TCGACCCAGT CAGAGGAGGA AGTGGATGAA CTCTCTCTGA TTGACCACAA TGAAATTATG 
GCTAGGTTGA CGTTAAAACA AGAGGGTGAT GATGGGCCAG ATGTCCGTGG GGGATCTGGG 
GATATATTGC TGGTCCATGC AACTGAGACC GATAGAAAAG ACTTGGTTTT GTACTGTGAA 
GCCTTTTTGA CAACCTACAG GACATTTATC ACCCCTGAGG AACTCATTAA GAAGCTACAG 
TATAGATATG AGAAGTTCTG CCCCTTCCCA GACACATTCA AGAAGCGAGT CAGCAAGAAT 
ACTTTCTTCG TGCTGGTGCG GGTAGTGGAT GAACTGTGCC TGGTGGAATT AACTGAAGAG 
ATCCTAAAGC TCTTGATGGA GCTGGTGTTC CGGTTAGTGT GCAGTGGGGA GCTGAGCCTT 
GCCCGGGTCC TCCGGAAGAA TATCTTGGAC AAAGTGAACC AAAAGAAGAT GCTAAAGTGT 
GCCAACTCGG ACCAACCCCT GGCTGCTCGA GGGGTGGCTG CCAGGCCTGG GACTCTGCAT 
GACTTCCACA GCCATGAGAT TGCTGAGCAG CTGACACTGT TGGATGCTGA GCTCTTCTAC 
AAGATAGAGA TCCCAGAAGT TTTGCTTTGG GCAAAAGAGC AGAATGAAGA GAAGAGCCCT 
AACTTGACAC AGTTTACTGA GCACTTTAAT AACATGTCCT ACTGGGTTCG TTCAATAATC 
ATGTTGCAAG AAAAAGCCCA GGACAGAGAA CGGCTGCTTC TGAAATTTAT CAAGATCATG 
AAGCACTTGA GGAAGCTAAA TAACTTCAAC TCGTATCTGG CTATCCTTTC AGCACTGGAC 
TCAGCACCCA TCCGGAGGCT GGAGTGGCAA AAACAGACCT CAGAGGGCCT GGCCGAGTAC 
TGCACTTTGA TTGACAGCTC TTCCTCCTTC CGGGCCTATC GGGCGGCTCT TTCTGAGGTG 
GAACCTCCAT GCATCCCATA CCTGGGTCTG ATCCTGCAGG ACCTCACCTT TGTTCACCTC 
GGAAACCCAG ACTACATTGA CGGGAAAGTG AATTTTTCCA AGCGCTGGCA GCAGTTCAAC 
ATCCTGGACA GTATGAGATG TTTCCAGCAG GCACATTATG AAATCCGCAG AAATGAGGAC 
ATCGTGAACT TCTTCAATGA TTTCAGTGAC CACCTAGCCG AGGAGGCCTT ATGGGAACTC 
TCTCTCAAAA TCAAACCCCG GAACATAACT CGGCGAAAAA CAGATCGAGA AGAGAAGACC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012409422.1
CDS	30..3092
Translation	MKLMDKFHSPKIKRTPSKKGKPADVPVKTFEKPVNKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQTDPRIQQSSAISSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKEVIKAVLDGVKELVRLTIEKQEHPSPTSPVKPSSPACKTDSQPELPLTDREMEILNKTTSMSQSTELLHPDSMDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSTADQIPQQVACNLSPLPESVGESGSPFHGRPFQLPPSNSPQPELGNPLPMGHPTDTPPALPEKKRRSAASQTTDSSSSSCRVSYERHPSQYDNIMEDDLQNPASLQPGSFTPFAAVLPFQQGGSSANFVSDFAAPDSMGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFNDSFTQELAPPPALPPKQRQLESPATKDGHPKDSSSHGGKENREGGERQLKSPDGLESTQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIKKLQYRYEKFCPFPDTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCSGELSLARVLRKNILDKVNQKKMLKCANSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYEIRRNEDIVNFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_012553968.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012553968.1

ORF Insert Sequence:

ATGAAGCTGA TGGACAAATT TCACTCGCCC AAAATCAAGA GAACACCATC AAAGAAAGGA 
AAACCAGCCG ATGTGCCTGT GAAAACTTTT GAGAAACCTG TGAACAAAAA TCTCAGCTGG 
CTGGAAGAGA AAGAAAAAGA GGTGGTCAGT GCCTTGCGCT ACTTTAAGAC CATTGTGGAC 
AAAATGGCAA TTGACAAGAA GGTACTGGAA ATGCTTCCAG GTTCGGCCAG CAAGGTCCTG 
GAGGCCATCC TGCCCCTAGT GCAGACAGAC CCTCGCATTC AGCAGAGCTC AGCCATCTCC 
TCTTGCTACA GCCGGGTGTA TCAGAGTCTA GCCAATCTCA TTCGTTGGTC AGATCAAGTG 
ATGCTGGAAG GTGTGAACTC GGAAGATAAA GAGATGGTGA CAACTGTGAA AGAAGTCATC 
AAAGCTGTTT TGGATGGAGT GAAGGAGCTT GTCAGGCTCA CAATAGAGAA GCAGGAGCAT 
CCATCTCCCA CAAGTCCAGT TAAGCCCAGC TCACCAGCCT GCAAAACTGA CAGCCAACCT 
GAGCTCCCAT TGACTGACCG GGAAATGGAG ATTCTCAACA AGACAACTAG TATGTCCCAA 
TCAACGGAGC TGCTGCATCC TGACTCCATG GATGAAGAGG TTGCTCCTCC CAAGCCCCCC 
CTCCCTGGCA TCCGAGTGGT GGATAATAGT CCTCCACCAG CTTTACCACC CAAAAAGAGG 
CAGTCGGCAC CATCTCCCAC CAGAGTGGCT GTGGTTGCAC CCATGAGCCG GGCCACCAGT 
GGCTCAAGTT TACCTGTTGG AATCAATAGA CAGGACTTTG ATGTGGACTG TTATGCCCAA 
AGGAGGCTGT CTGGGGGCAG CCATTCATAT GGCGGGGAAT CCCCTCGCCT CTCCCCGTGC 
AGCAGCATCG GCAAGCTGAG CAAGTCCGAC GAACAGCTGT CCTCCTTGGA CCGGGACAGT 
GGCCAGTGCT CCAGGAATAC AAGCTGTGAA ACACTAGATC ACTATGACCC TGATTATGAA 
TTTCTACAGC AAGACCTCTC CACTGCTGAC CAGATACCTC AGCAAGTAGC ATGTAACCTT 
AGCCCATTGC CAGAATCTGT TGGTGAATCT GGCTCTCCTT TCCATGGACG TCCTTTCCAG 
CTGCCACCCA GCAACTCCCC ACAGCCAGAG CTTGGCAATC CCTTGCCCAT GGGGCATCCT 
ACGGACACAC CTCCTGCCCT GCCTGAGAAG AAGCGGAGGA GTGCAGCCTC TCAGACCACG 
GACAGCTCCA GCTCCAGCTG TAGAGTCTCC TATGAGAGGC ACCCTTCCCA ATATGACAAT 
ATCATGGAGG ATGATCTCCA GAACCCGGCC TCCCTCCAGC CAGGATCATT CACGCCATTC 
GCTGCTGTCC TCCCCTTCCA GCAAGGAGGC TCTTCAGCCA ATTTTGTGTC TGATTTTGCT 
GCTCCCGATT CAATGGGTGA CCCCGAGAAA CCACCTCCTC TACCTGAAAA GAAGAACAAA 
CACATGCTGG CCTACATGCA GCTCCTTGAG GACTACTCAG AGCCCCAGCC TTCCATGTTC 
TACCAGACGC CCCAGAACGA GCACATCTAC CAGCAGAAGA ACAAGCTCCT CATGGAGGTC 
TACGGCTTTA ATGACTCCTT CACCCAGGAG CTGGCCCCGC CCCCTGCCCT GCCCCCCAAG 
CAGAGGCAGC TGGAATCACC AGCTACAAAA GATGGACATC CCAAAGATTC CTCTTCACAT 
GGTGGGAAAG AAAACAGAGA AGGTGGTGAA AGGCAGCTGA AGTCCCCCGA TGGTTTGGAA 
TCGACCCAGT CAGAGGAGGA AGTGGATGAA CTCTCTCTGA TTGACCACAA TGAAATTATG 
GCTAGGTTGA CGTTAAAACA AGAGGGTGAT GATGGGCCAG ATGTCCGTGG GGGATCTGGG 
GATATATTGC TGGTCCATGC AACTGAGACC GATAGAAAAG ACTTGGTTTT GTACTGTGAA 
GCCTTTTTGA CAACCTACAG GACATTTATC ACCCCTGAGG AACTCATTAA GAAGCTACAG 
TATAGATATG AGAAGTTCTG CCCCTTCCCA GACACATTCA AGAAGCGAGT CAGCAAGAAT 
ACTTTCTTCG TGCTGGTGCG GGTAGTGGAT GAACTGTGCC TGGTGGAATT AACTGAAGAG 
ATCCTAAAGC TCTTGATGGA GCTGGTGTTC CGGTTAGTGT GCAGTGGGGA GCTGAGCCTT 
GCCCGGGTCC TCCGGAAGAA TATCTTGGAC AAAGTGAACC AAAAGAAGAT GCTAAAGTGT 
GCCAACTCGG ACCAACCCCT GGCTGCTCGA GGGGTGGCTG CCAGGCCTGG GACTCTGCAT 
GACTTCCACA GCCATGAGAT TGCTGAGCAG CTGACACTGT TGGATGCTGA GCTCTTCTAC 
AAGATAGAGA TCCCAGAAGT TTTGCTTTGG GCAAAAGAGC AGAATGAAGA GAAGAGCCCT 
AACTTGACAC AGTTTACTGA GCACTTTAAT AACATGTCCT ACTGGGTTCG TTCAATAATC 
ATGTTGCAAG AAAAAGCCCA GGACAGAGAA CGGCTGCTTC TGAAATTTAT CAAGATCATG 
AAGCACTTGA GGAAGCTAAA TAACTTCAAC TCGTATCTGG CTATCCTTTC AGCACTGGAC 
TCAGCACCCA TCCGGAGGCT GGAGTGGCAA AAACAGACCT CAGAGGGCCT GGCCGAGTAC 
TGCACTTTGA TTGACAGCTC TTCCTCCTTC CGGGCCTATC GGGCGGCTCT TTCTGAGGTG 
GAACCTCCAT GCATCCCATA CCTGGGTCTG ATCCTGCAGG ACCTCACCTT TGTTCACCTC 
GGAAACCCAG ACTACATTGA CGGGAAAGTG AATTTTTCCA AGCGCTGGCA GCAGTTCAAC 
ATCCTGGACA GTATGAGATG TTTCCAGCAG GCACATTATG AAATCCGCAG AAATGAGGAC 
ATCGTGAACT TCTTCAATGA TTTCAGTGAC CACCTAGCCG AGGAGGCCTT ATGGGAACTC 
TCTCTCAAAA TCAAACCCCG GAACATAACT CGGCGAAAAA CAGATCGAGA AGAGAAGACC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_012553967.2 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_012409421.2 Sarcophilus harrisii Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSa150375
Clone ID Related Accession (Same CDS sequence)	XM_012553967.2
Accession Version	XM_012553967.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3234bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515513600000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	rap guanine nucleotide exchange factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003816662.1) and transcript sequence (GEDN01112064.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jan 11, 2018 this sequence version replaced XM_012553967.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 453 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGTCTGGCA AGATCGAGAA AGCAGATTCC CAGCGCTCTC ATCTCTCCTC CTTCACCATG 
AAGCTGATGG ACAAATTTCA CTCGCCCAAA ATCAAGAGAA CACCATCAAA GAAAGGAAAA 
CCAGCCGATG TGCCTGTGAA AACTTTTGAG AAACCTGTGA ACAAAGAGGC CACAGACAAT 
TTTCTACCAG AGGGCTACCC TCCTCCCTTG GATCTGGATC ACCAAGCAGT ACAATTTATG 
TCCACCAGTG CTGTGGTTCC CAGGTCTCAG AGGCAGAAGA ATCTCAGCTG GCTGGAAGAG 
AAAGAAAAAG AGGTGGTCAG TGCCTTGCGC TACTTTAAGA CCATTGTGGA CAAAATGGCA 
ATTGACAAGA AGGTACTGGA AATGCTTCCA GGTTCGGCCA GCAAGGTCCT GGAGGCCATC 
CTGCCCCTAG TGCAGACAGA CCCTCGCATT CAGCAGAGCT CAGCCATCTC CTCTTGCTAC 
AGCCGGGTGT ATCAGAGTCT AGCCAATCTC ATTCGTTGGT CAGATCAAGT GATGCTGGAA 
GGTGTGAACT CGGAAGATAA AGAGATGGTG ACAACTGTGA AAGAAGTCAT CAAAGCTGTT 
TTGGATGGAG TGAAGGAGCT TGTCAGGCTC ACAATAGAGA AGCAGGAGCA TCCATCTCCC 
ACAAGTCCAG TTAAGCCCAG CTCACCAGCC TGCAAAACTG ACAGCCAACC TGAGCTCCCA 
TTGACTGACC GGGAAATGGA GATTCTCAAC AAGACAACTA GTATGTCCCA ATCAACGGAG 
CTGCTGCATC CTGACTCCAT GGATGAAGAG GTTGCTCCTC CCAAGCCCCC CCTCCCTGGC 
ATCCGAGTGG TGGATAATAG TCCTCCACCA GCTTTACCAC CCAAAAAGAG GCAGTCGGCA 
CCATCTCCCA CCAGAGTGGC TGTGGTTGCA CCCATGAGCC GGGCCACCAG TGGCTCAAGT 
TTACCTGTTG GAATCAATAG ACAGGACTTT GATGTGGACT GTTATGCCCA AAGGAGGCTG 
TCTGGGGGCA GCCATTCATA TGGCGGGGAA TCCCCTCGCC TCTCCCCGTG CAGCAGCATC 
GGCAAGCTGA GCAAGTCCGA CGAACAGCTG TCCTCCTTGG ACCGGGACAG TGGCCAGTGC 
TCCAGGAATA CAAGCTGTGA AACACTAGAT CACTATGACC CTGATTATGA ATTTCTACAG 
CAAGACCTCT CCACTGCTGA CCAGATACCT CAGCAAGTAG CATGTAACCT TAGCCCATTG 
CCAGAATCTG TTGGTGAATC TGGCTCTCCT TTCCATGGAC GTCCTTTCCA GCTGCCACCC 
AGCAACTCCC CACAGCCAGA GCTTGGCAAT CCCTTGCCCA TGGGGCATCC TACGGACACA 
CCTCCTGCCC TGCCTGAGAA GAAGCGGAGG AGTGCAGCCT CTCAGACCAC GGACAGCTCC 
AGCTCCAGCT GTAGAGTCTC CTATGAGAGG CACCCTTCCC AATATGACAA TATCATGGAG 
GATGATCTCC AGAACCCGGC CTCCCTCCAG CCAGGATCAT TCACGCCATT CGCTGCTGTC 
CTCCCCTTCC AGCAAGGAGG CTCTTCAGCC AATTTTGTGT CTGATTTTGC TGCTCCCGAT 
TCAATGGGTG ACCCCGAGAA ACCACCTCCT CTACCTGAAA AGAAGAACAA ACACATGCTG 
GCCTACATGC AGCTCCTTGA GGACTACTCA GAGCCCCAGC CTTCCATGTT CTACCAGACG 
CCCCAGAACG AGCACATCTA CCAGCAGAAG AACAAGCTCC TCATGGAGGT CTACGGCTTT 
AATGACTCCT TCACCCAGGA GCTGGCCCCG CCCCCTGCCC TGCCCCCCAA GCAGAGGCAG 
CTGGAATCAC CAGCTACAAA AGATGGACAT CCCAAAGATT CCTCTTCACA TGGTGGGAAA 
GAAAACAGAG AAGGTGGTGA AAGGCAGCTG AAGTCCCCCG ATGGTTTGGA ATCGACCCAG 
TCAGAGGAGG AAGTGGATGA ACTCTCTCTG ATTGACCACA ATGAAATTAT GGCTAGGTTG 
ACGTTAAAAC AAGAGGGTGA TGATGGGCCA GATGTCCGTG GGGGATCTGG GGATATATTG 
CTGGTCCATG CAACTGAGAC CGATAGAAAA GACTTGGTTT TGTACTGTGA AGCCTTTTTG 
ACAACCTACA GGACATTTAT CACCCCTGAG GAACTCATTA AGAAGCTACA GTATAGATAT 
GAGAAGTTCT GCCCCTTCCC AGACACATTC AAGAAGCGAG TCAGCAAGAA TACTTTCTTC 
GTGCTGGTGC GGGTAGTGGA TGAACTGTGC CTGGTGGAAT TAACTGAAGA GATCCTAAAG 
CTCTTGATGG AGCTGGTGTT CCGGTTAGTG TGCAGTGGGG AGCTGAGCCT TGCCCGGGTC 
CTCCGGAAGA ATATCTTGGA CAAAGTGAAC CAAAAGAAGA TGCTAAAGTG TGCCAACTCG 
GACCAACCCC TGGCTGCTCG AGGGGTGGCT GCCAGGCCTG GGACTCTGCA TGACTTCCAC 
AGCCATGAGA TTGCTGAGCA GCTGACACTG TTGGATGCTG AGCTCTTCTA CAAGATAGAG 
ATCCCAGAAG TTTTGCTTTG GGCAAAAGAG CAGAATGAAG AGAAGAGCCC TAACTTGACA 
CAGTTTACTG AGCACTTTAA TAACATGTCC TACTGGGTTC GTTCAATAAT CATGTTGCAA 
GAAAAAGCCC AGGACAGAGA ACGGCTGCTT CTGAAATTTA TCAAGATCAT GAAGCACTTG 
AGGAAGCTAA ATAACTTCAA CTCGTATCTG GCTATCCTTT CAGCACTGGA CTCAGCACCC 
ATCCGGAGGC TGGAGTGGCA AAAACAGACC TCAGAGGGCC TGGCCGAGTA CTGCACTTTG 
ATTGACAGCT CTTCCTCCTT CCGGGCCTAT CGGGCGGCTC TTTCTGAGGT GGAACCTCCA 
TGCATCCCAT ACCTGGGTCT GATCCTGCAG GACCTCACCT TTGTTCACCT CGGAAACCCA 
GACTACATTG ACGGGAAAGT GAATTTTTCC AAGCGCTGGC AGCAGTTCAA CATCCTGGAC 
AGTATGAGAT GTTTCCAGCA GGCACATTAT GAAATCCGCA GAAATGAGGA CATCGTGAAC 
TTCTTCAATG ATTTCAGTGA CCACCTAGCC GAGGAGGCCT TATGGGAACT CTCTCTCAAA 
ATCAAACCCC GGAACATAAC TCGGCGAAAA ACAGATCGAG AAGAGAAGAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012409421.2
CDS	429..3662
Translation	MSGKIEKADSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPADVPVKTFEKPVNKEATDNFLPEGYPPPLDLDHQAVQFMSTSAVVPRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQTDPRIQQSSAISSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKEVIKAVLDGVKELVRLTIEKQEHPSPTSPVKPSSPACKTDSQPELPLTDREMEILNKTTSMSQSTELLHPDSMDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSTADQIPQQVACNLSPLPESVGESGSPFHGRPFQLPPSNSPQPELGNPLPMGHPTDTPPALPEKKRRSAASQTTDSSSSSCRVSYERHPSQYDNIMEDDLQNPASLQPGSFTPFAAVLPFQQGGSSANFVSDFAAPDSMGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFNDSFTQELAPPPALPPKQRQLESPATKDGHPKDSSSHGGKENREGGERQLKSPDGLESTQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIKKLQYRYEKFCPFPDTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCSGELSLARVLRKNILDKVNQKKMLKCANSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYEIRRNEDIVNFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_012553967.2
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012553967.2

ORF Insert Sequence:

ATGTCTGGCA AGATCGAGAA AGCAGATTCC CAGCGCTCTC ATCTCTCCTC CTTCACCATG 
AAGCTGATGG ACAAATTTCA CTCGCCCAAA ATCAAGAGAA CACCATCAAA GAAAGGAAAA 
CCAGCCGATG TGCCTGTGAA AACTTTTGAG AAACCTGTGA ACAAAGAGGC CACAGACAAT 
TTTCTACCAG AGGGCTACCC TCCTCCCTTG GATCTGGATC ACCAAGCAGT ACAATTTATG 
TCCACCAGTG CTGTGGTTCC CAGGTCTCAG AGGCAGAAGA ATCTCAGCTG GCTGGAAGAG 
AAAGAAAAAG AGGTGGTCAG TGCCTTGCGC TACTTTAAGA CCATTGTGGA CAAAATGGCA 
ATTGACAAGA AGGTACTGGA AATGCTTCCA GGTTCGGCCA GCAAGGTCCT GGAGGCCATC 
CTGCCCCTAG TGCAGACAGA CCCTCGCATT CAGCAGAGCT CAGCCATCTC CTCTTGCTAC 
AGCCGGGTGT ATCAGAGTCT AGCCAATCTC ATTCGTTGGT CAGATCAAGT GATGCTGGAA 
GGTGTGAACT CGGAAGATAA AGAGATGGTG ACAACTGTGA AAGAAGTCAT CAAAGCTGTT 
TTGGATGGAG TGAAGGAGCT TGTCAGGCTC ACAATAGAGA AGCAGGAGCA TCCATCTCCC 
ACAAGTCCAG TTAAGCCCAG CTCACCAGCC TGCAAAACTG ACAGCCAACC TGAGCTCCCA 
TTGACTGACC GGGAAATGGA GATTCTCAAC AAGACAACTA GTATGTCCCA ATCAACGGAG 
CTGCTGCATC CTGACTCCAT GGATGAAGAG GTTGCTCCTC CCAAGCCCCC CCTCCCTGGC 
ATCCGAGTGG TGGATAATAG TCCTCCACCA GCTTTACCAC CCAAAAAGAG GCAGTCGGCA 
CCATCTCCCA CCAGAGTGGC TGTGGTTGCA CCCATGAGCC GGGCCACCAG TGGCTCAAGT 
TTACCTGTTG GAATCAATAG ACAGGACTTT GATGTGGACT GTTATGCCCA AAGGAGGCTG 
TCTGGGGGCA GCCATTCATA TGGCGGGGAA TCCCCTCGCC TCTCCCCGTG CAGCAGCATC 
GGCAAGCTGA GCAAGTCCGA CGAACAGCTG TCCTCCTTGG ACCGGGACAG TGGCCAGTGC 
TCCAGGAATA CAAGCTGTGA AACACTAGAT CACTATGACC CTGATTATGA ATTTCTACAG 
CAAGACCTCT CCACTGCTGA CCAGATACCT CAGCAAGTAG CATGTAACCT TAGCCCATTG 
CCAGAATCTG TTGGTGAATC TGGCTCTCCT TTCCATGGAC GTCCTTTCCA GCTGCCACCC 
AGCAACTCCC CACAGCCAGA GCTTGGCAAT CCCTTGCCCA TGGGGCATCC TACGGACACA 
CCTCCTGCCC TGCCTGAGAA GAAGCGGAGG AGTGCAGCCT CTCAGACCAC GGACAGCTCC 
AGCTCCAGCT GTAGAGTCTC CTATGAGAGG CACCCTTCCC AATATGACAA TATCATGGAG 
GATGATCTCC AGAACCCGGC CTCCCTCCAG CCAGGATCAT TCACGCCATT CGCTGCTGTC 
CTCCCCTTCC AGCAAGGAGG CTCTTCAGCC AATTTTGTGT CTGATTTTGC TGCTCCCGAT 
TCAATGGGTG ACCCCGAGAA ACCACCTCCT CTACCTGAAA AGAAGAACAA ACACATGCTG 
GCCTACATGC AGCTCCTTGA GGACTACTCA GAGCCCCAGC CTTCCATGTT CTACCAGACG 
CCCCAGAACG AGCACATCTA CCAGCAGAAG AACAAGCTCC TCATGGAGGT CTACGGCTTT 
AATGACTCCT TCACCCAGGA GCTGGCCCCG CCCCCTGCCC TGCCCCCCAA GCAGAGGCAG 
CTGGAATCAC CAGCTACAAA AGATGGACAT CCCAAAGATT CCTCTTCACA TGGTGGGAAA 
GAAAACAGAG AAGGTGGTGA AAGGCAGCTG AAGTCCCCCG ATGGTTTGGA ATCGACCCAG 
TCAGAGGAGG AAGTGGATGA ACTCTCTCTG ATTGACCACA ATGAAATTAT GGCTAGGTTG 
ACGTTAAAAC AAGAGGGTGA TGATGGGCCA GATGTCCGTG GGGGATCTGG GGATATATTG 
CTGGTCCATG CAACTGAGAC CGATAGAAAA GACTTGGTTT TGTACTGTGA AGCCTTTTTG 
ACAACCTACA GGACATTTAT CACCCCTGAG GAACTCATTA AGAAGCTACA GTATAGATAT 
GAGAAGTTCT GCCCCTTCCC AGACACATTC AAGAAGCGAG TCAGCAAGAA TACTTTCTTC 
GTGCTGGTGC GGGTAGTGGA TGAACTGTGC CTGGTGGAAT TAACTGAAGA GATCCTAAAG 
CTCTTGATGG AGCTGGTGTT CCGGTTAGTG TGCAGTGGGG AGCTGAGCCT TGCCCGGGTC 
CTCCGGAAGA ATATCTTGGA CAAAGTGAAC CAAAAGAAGA TGCTAAAGTG TGCCAACTCG 
GACCAACCCC TGGCTGCTCG AGGGGTGGCT GCCAGGCCTG GGACTCTGCA TGACTTCCAC 
AGCCATGAGA TTGCTGAGCA GCTGACACTG TTGGATGCTG AGCTCTTCTA CAAGATAGAG 
ATCCCAGAAG TTTTGCTTTG GGCAAAAGAG CAGAATGAAG AGAAGAGCCC TAACTTGACA 
CAGTTTACTG AGCACTTTAA TAACATGTCC TACTGGGTTC GTTCAATAAT CATGTTGCAA 
GAAAAAGCCC AGGACAGAGA ACGGCTGCTT CTGAAATTTA TCAAGATCAT GAAGCACTTG 
AGGAAGCTAA ATAACTTCAA CTCGTATCTG GCTATCCTTT CAGCACTGGA CTCAGCACCC 
ATCCGGAGGC TGGAGTGGCA AAAACAGACC TCAGAGGGCC TGGCCGAGTA CTGCACTTTG 
ATTGACAGCT CTTCCTCCTT CCGGGCCTAT CGGGCGGCTC TTTCTGAGGT GGAACCTCCA 
TGCATCCCAT ACCTGGGTCT GATCCTGCAG GACCTCACCT TTGTTCACCT CGGAAACCCA 
GACTACATTG ACGGGAAAGT GAATTTTTCC AAGCGCTGGC AGCAGTTCAA CATCCTGGAC 
AGTATGAGAT GTTTCCAGCA GGCACATTAT GAAATCCGCA GAAATGAGGA CATCGTGAAC 
TTCTTCAATG ATTTCAGTGA CCACCTAGCC GAGGAGGCCT TATGGGAACT CTCTCTCAAA 
ATCAAACCCC GGAACATAAC TCGGCGAAAA ACAGATCGAG AAGAGAAGAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_012553968.2 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_012409422.2 Sarcophilus harrisii Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSa150376
Clone ID Related Accession (Same CDS sequence)	XM_012553968.2
Accession Version	XM_012553968.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3120bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515513600000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	rap guanine nucleotide exchange factor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003816662.1) and transcript sequence (GEDN01112064.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jan 11, 2018 this sequence version replaced XM_012553968.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 453 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGTCTGGCA AGATCGAGAA AGCAGATTCC CAGCGCTCTC ATCTCTCCTC CTTCACCATG 
AAGCTGATGG ACAAATTTCA CTCGCCCAAA ATCAAGAGAA CACCATCAAA GAAAGGAAAA 
CCAGCCGATG TGCCTGTGAA AACTTTTGAG AAACCTGTGA ACAAAAATCT CAGCTGGCTG 
GAAGAGAAAG AAAAAGAGGT GGTCAGTGCC TTGCGCTACT TTAAGACCAT TGTGGACAAA 
ATGGCAATTG ACAAGAAGGT ACTGGAAATG CTTCCAGGTT CGGCCAGCAA GGTCCTGGAG 
GCCATCCTGC CCCTAGTGCA GACAGACCCT CGCATTCAGC AGAGCTCAGC CATCTCCTCT 
TGCTACAGCC GGGTGTATCA GAGTCTAGCC AATCTCATTC GTTGGTCAGA TCAAGTGATG 
CTGGAAGGTG TGAACTCGGA AGATAAAGAG ATGGTGACAA CTGTGAAAGA AGTCATCAAA 
GCTGTTTTGG ATGGAGTGAA GGAGCTTGTC AGGCTCACAA TAGAGAAGCA GGAGCATCCA 
TCTCCCACAA GTCCAGTTAA GCCCAGCTCA CCAGCCTGCA AAACTGACAG CCAACCTGAG 
CTCCCATTGA CTGACCGGGA AATGGAGATT CTCAACAAGA CAACTAGTAT GTCCCAATCA 
ACGGAGCTGC TGCATCCTGA CTCCATGGAT GAAGAGGTTG CTCCTCCCAA GCCCCCCCTC 
CCTGGCATCC GAGTGGTGGA TAATAGTCCT CCACCAGCTT TACCACCCAA AAAGAGGCAG 
TCGGCACCAT CTCCCACCAG AGTGGCTGTG GTTGCACCCA TGAGCCGGGC CACCAGTGGC 
TCAAGTTTAC CTGTTGGAAT CAATAGACAG GACTTTGATG TGGACTGTTA TGCCCAAAGG 
AGGCTGTCTG GGGGCAGCCA TTCATATGGC GGGGAATCCC CTCGCCTCTC CCCGTGCAGC 
AGCATCGGCA AGCTGAGCAA GTCCGACGAA CAGCTGTCCT CCTTGGACCG GGACAGTGGC 
CAGTGCTCCA GGAATACAAG CTGTGAAACA CTAGATCACT ATGACCCTGA TTATGAATTT 
CTACAGCAAG ACCTCTCCAC TGCTGACCAG ATACCTCAGC AAGTAGCATG TAACCTTAGC 
CCATTGCCAG AATCTGTTGG TGAATCTGGC TCTCCTTTCC ATGGACGTCC TTTCCAGCTG 
CCACCCAGCA ACTCCCCACA GCCAGAGCTT GGCAATCCCT TGCCCATGGG GCATCCTACG 
GACACACCTC CTGCCCTGCC TGAGAAGAAG CGGAGGAGTG CAGCCTCTCA GACCACGGAC 
AGCTCCAGCT CCAGCTGTAG AGTCTCCTAT GAGAGGCACC CTTCCCAATA TGACAATATC 
ATGGAGGATG ATCTCCAGAA CCCGGCCTCC CTCCAGCCAG GATCATTCAC GCCATTCGCT 
GCTGTCCTCC CCTTCCAGCA AGGAGGCTCT TCAGCCAATT TTGTGTCTGA TTTTGCTGCT 
CCCGATTCAA TGGGTGACCC CGAGAAACCA CCTCCTCTAC CTGAAAAGAA GAACAAACAC 
ATGCTGGCCT ACATGCAGCT CCTTGAGGAC TACTCAGAGC CCCAGCCTTC CATGTTCTAC 
CAGACGCCCC AGAACGAGCA CATCTACCAG CAGAAGAACA AGCTCCTCAT GGAGGTCTAC 
GGCTTTAATG ACTCCTTCAC CCAGGAGCTG GCCCCGCCCC CTGCCCTGCC CCCCAAGCAG 
AGGCAGCTGG AATCACCAGC TACAAAAGAT GGACATCCCA AAGATTCCTC TTCACATGGT 
GGGAAAGAAA ACAGAGAAGG TGGTGAAAGG CAGCTGAAGT CCCCCGATGG TTTGGAATCG 
ACCCAGTCAG AGGAGGAAGT GGATGAACTC TCTCTGATTG ACCACAATGA AATTATGGCT 
AGGTTGACGT TAAAACAAGA GGGTGATGAT GGGCCAGATG TCCGTGGGGG ATCTGGGGAT 
ATATTGCTGG TCCATGCAAC TGAGACCGAT AGAAAAGACT TGGTTTTGTA CTGTGAAGCC 
TTTTTGACAA CCTACAGGAC ATTTATCACC CCTGAGGAAC TCATTAAGAA GCTACAGTAT 
AGATATGAGA AGTTCTGCCC CTTCCCAGAC ACATTCAAGA AGCGAGTCAG CAAGAATACT 
TTCTTCGTGC TGGTGCGGGT AGTGGATGAA CTGTGCCTGG TGGAATTAAC TGAAGAGATC 
CTAAAGCTCT TGATGGAGCT GGTGTTCCGG TTAGTGTGCA GTGGGGAGCT GAGCCTTGCC 
CGGGTCCTCC GGAAGAATAT CTTGGACAAA GTGAACCAAA AGAAGATGCT AAAGTGTGCC 
AACTCGGACC AACCCCTGGC TGCTCGAGGG GTGGCTGCCA GGCCTGGGAC TCTGCATGAC 
TTCCACAGCC ATGAGATTGC TGAGCAGCTG ACACTGTTGG ATGCTGAGCT CTTCTACAAG 
ATAGAGATCC CAGAAGTTTT GCTTTGGGCA AAAGAGCAGA ATGAAGAGAA GAGCCCTAAC 
TTGACACAGT TTACTGAGCA CTTTAATAAC ATGTCCTACT GGGTTCGTTC AATAATCATG 
TTGCAAGAAA AAGCCCAGGA CAGAGAACGG CTGCTTCTGA AATTTATCAA GATCATGAAG 
CACTTGAGGA AGCTAAATAA CTTCAACTCG TATCTGGCTA TCCTTTCAGC ACTGGACTCA 
GCACCCATCC GGAGGCTGGA GTGGCAAAAA CAGACCTCAG AGGGCCTGGC CGAGTACTGC 
ACTTTGATTG ACAGCTCTTC CTCCTTCCGG GCCTATCGGG CGGCTCTTTC TGAGGTGGAA 
CCTCCATGCA TCCCATACCT GGGTCTGATC CTGCAGGACC TCACCTTTGT TCACCTCGGA 
AACCCAGACT ACATTGACGG GAAAGTGAAT TTTTCCAAGC GCTGGCAGCA GTTCAACATC 
CTGGACAGTA TGAGATGTTT CCAGCAGGCA CATTATGAAA TCCGCAGAAA TGAGGACATC 
GTGAACTTCT TCAATGATTT CAGTGACCAC CTAGCCGAGG AGGCCTTATG GGAACTCTCT 
CTCAAAATCA AACCCCGGAA CATAACTCGG CGAAAAACAG ATCGAGAAGA GAAGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012409422.2
CDS	429..3548
Translation	MSGKIEKADSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPADVPVKTFEKPVNKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQTDPRIQQSSAISSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKEVIKAVLDGVKELVRLTIEKQEHPSPTSPVKPSSPACKTDSQPELPLTDREMEILNKTTSMSQSTELLHPDSMDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSTADQIPQQVACNLSPLPESVGESGSPFHGRPFQLPPSNSPQPELGNPLPMGHPTDTPPALPEKKRRSAASQTTDSSSSSCRVSYERHPSQYDNIMEDDLQNPASLQPGSFTPFAAVLPFQQGGSSANFVSDFAAPDSMGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFNDSFTQELAPPPALPPKQRQLESPATKDGHPKDSSSHGGKENREGGERQLKSPDGLESTQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIKKLQYRYEKFCPFPDTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCSGELSLARVLRKNILDKVNQKKMLKCANSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYEIRRNEDIVNFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_012553968.2
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012553968.2

ORF Insert Sequence:

ATGTCTGGCA AGATCGAGAA AGCAGATTCC CAGCGCTCTC ATCTCTCCTC CTTCACCATG 
AAGCTGATGG ACAAATTTCA CTCGCCCAAA ATCAAGAGAA CACCATCAAA GAAAGGAAAA 
CCAGCCGATG TGCCTGTGAA AACTTTTGAG AAACCTGTGA ACAAAAATCT CAGCTGGCTG 
GAAGAGAAAG AAAAAGAGGT GGTCAGTGCC TTGCGCTACT TTAAGACCAT TGTGGACAAA 
ATGGCAATTG ACAAGAAGGT ACTGGAAATG CTTCCAGGTT CGGCCAGCAA GGTCCTGGAG 
GCCATCCTGC CCCTAGTGCA GACAGACCCT CGCATTCAGC AGAGCTCAGC CATCTCCTCT 
TGCTACAGCC GGGTGTATCA GAGTCTAGCC AATCTCATTC GTTGGTCAGA TCAAGTGATG 
CTGGAAGGTG TGAACTCGGA AGATAAAGAG ATGGTGACAA CTGTGAAAGA AGTCATCAAA 
GCTGTTTTGG ATGGAGTGAA GGAGCTTGTC AGGCTCACAA TAGAGAAGCA GGAGCATCCA 
TCTCCCACAA GTCCAGTTAA GCCCAGCTCA CCAGCCTGCA AAACTGACAG CCAACCTGAG 
CTCCCATTGA CTGACCGGGA AATGGAGATT CTCAACAAGA CAACTAGTAT GTCCCAATCA 
ACGGAGCTGC TGCATCCTGA CTCCATGGAT GAAGAGGTTG CTCCTCCCAA GCCCCCCCTC 
CCTGGCATCC GAGTGGTGGA TAATAGTCCT CCACCAGCTT TACCACCCAA AAAGAGGCAG 
TCGGCACCAT CTCCCACCAG AGTGGCTGTG GTTGCACCCA TGAGCCGGGC CACCAGTGGC 
TCAAGTTTAC CTGTTGGAAT CAATAGACAG GACTTTGATG TGGACTGTTA TGCCCAAAGG 
AGGCTGTCTG GGGGCAGCCA TTCATATGGC GGGGAATCCC CTCGCCTCTC CCCGTGCAGC 
AGCATCGGCA AGCTGAGCAA GTCCGACGAA CAGCTGTCCT CCTTGGACCG GGACAGTGGC 
CAGTGCTCCA GGAATACAAG CTGTGAAACA CTAGATCACT ATGACCCTGA TTATGAATTT 
CTACAGCAAG ACCTCTCCAC TGCTGACCAG ATACCTCAGC AAGTAGCATG TAACCTTAGC 
CCATTGCCAG AATCTGTTGG TGAATCTGGC TCTCCTTTCC ATGGACGTCC TTTCCAGCTG 
CCACCCAGCA ACTCCCCACA GCCAGAGCTT GGCAATCCCT TGCCCATGGG GCATCCTACG 
GACACACCTC CTGCCCTGCC TGAGAAGAAG CGGAGGAGTG CAGCCTCTCA GACCACGGAC 
AGCTCCAGCT CCAGCTGTAG AGTCTCCTAT GAGAGGCACC CTTCCCAATA TGACAATATC 
ATGGAGGATG ATCTCCAGAA CCCGGCCTCC CTCCAGCCAG GATCATTCAC GCCATTCGCT 
GCTGTCCTCC CCTTCCAGCA AGGAGGCTCT TCAGCCAATT TTGTGTCTGA TTTTGCTGCT 
CCCGATTCAA TGGGTGACCC CGAGAAACCA CCTCCTCTAC CTGAAAAGAA GAACAAACAC 
ATGCTGGCCT ACATGCAGCT CCTTGAGGAC TACTCAGAGC CCCAGCCTTC CATGTTCTAC 
CAGACGCCCC AGAACGAGCA CATCTACCAG CAGAAGAACA AGCTCCTCAT GGAGGTCTAC 
GGCTTTAATG ACTCCTTCAC CCAGGAGCTG GCCCCGCCCC CTGCCCTGCC CCCCAAGCAG 
AGGCAGCTGG AATCACCAGC TACAAAAGAT GGACATCCCA AAGATTCCTC TTCACATGGT 
GGGAAAGAAA ACAGAGAAGG TGGTGAAAGG CAGCTGAAGT CCCCCGATGG TTTGGAATCG 
ACCCAGTCAG AGGAGGAAGT GGATGAACTC TCTCTGATTG ACCACAATGA AATTATGGCT 
AGGTTGACGT TAAAACAAGA GGGTGATGAT GGGCCAGATG TCCGTGGGGG ATCTGGGGAT 
ATATTGCTGG TCCATGCAAC TGAGACCGAT AGAAAAGACT TGGTTTTGTA CTGTGAAGCC 
TTTTTGACAA CCTACAGGAC ATTTATCACC CCTGAGGAAC TCATTAAGAA GCTACAGTAT 
AGATATGAGA AGTTCTGCCC CTTCCCAGAC ACATTCAAGA AGCGAGTCAG CAAGAATACT 
TTCTTCGTGC TGGTGCGGGT AGTGGATGAA CTGTGCCTGG TGGAATTAAC TGAAGAGATC 
CTAAAGCTCT TGATGGAGCT GGTGTTCCGG TTAGTGTGCA GTGGGGAGCT GAGCCTTGCC 
CGGGTCCTCC GGAAGAATAT CTTGGACAAA GTGAACCAAA AGAAGATGCT AAAGTGTGCC 
AACTCGGACC AACCCCTGGC TGCTCGAGGG GTGGCTGCCA GGCCTGGGAC TCTGCATGAC 
TTCCACAGCC ATGAGATTGC TGAGCAGCTG ACACTGTTGG ATGCTGAGCT CTTCTACAAG 
ATAGAGATCC CAGAAGTTTT GCTTTGGGCA AAAGAGCAGA ATGAAGAGAA GAGCCCTAAC 
TTGACACAGT TTACTGAGCA CTTTAATAAC ATGTCCTACT GGGTTCGTTC AATAATCATG 
TTGCAAGAAA AAGCCCAGGA CAGAGAACGG CTGCTTCTGA AATTTATCAA GATCATGAAG 
CACTTGAGGA AGCTAAATAA CTTCAACTCG TATCTGGCTA TCCTTTCAGC ACTGGACTCA 
GCACCCATCC GGAGGCTGGA GTGGCAAAAA CAGACCTCAG AGGGCCTGGC CGAGTACTGC 
ACTTTGATTG ACAGCTCTTC CTCCTTCCGG GCCTATCGGG CGGCTCTTTC TGAGGTGGAA 
CCTCCATGCA TCCCATACCT GGGTCTGATC CTGCAGGACC TCACCTTTGT TCACCTCGGA 
AACCCAGACT ACATTGACGG GAAAGTGAAT TTTTCCAAGC GCTGGCAGCA GTTCAACATC 
CTGGACAGTA TGAGATGTTT CCAGCAGGCA CATTATGAAA TCCGCAGAAA TGAGGACATC 
GTGAACTTCT TCAATGATTT CAGTGACCAC CTAGCCGAGG AGGCCTTATG GGAACTCTCT 
CTCAAAATCA AACCCCGGAA CATAACTCGG CGAAAAACAG ATCGAGAAGA GAAGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_031954874.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031810734.1 Sarcophilus harrisii Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X1, mRNA.

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CloneID	OSa226538
Clone ID Related Accession (Same CDS sequence)	XM_031954874.1
Accession Version	XM_031954874.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3705bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	rap guanine nucleotide exchange factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045427.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAATG TTAATGAAAA ACAAAAAGCT TGGAAGCAGA GCAATTTATA CCTCTGGAAA 
CAAGATTCCC AGCGCTCTCA TCTCTCCTCC TTCACCATGA AGCTGATGGA CAAATTTCAC 
TCGCCCAAAA TCAAGAGAAC ACCATCAAAG AAAGGAAAAC CAGCCGATGT GCCTGTGAAA 
ACTTTTGAGA AACCTGTGAA CAAAGAGGCC ACAGACAATT TTCTACCAGA GGGCTACCCT 
CCTCCCTTGG ATCTGGATCA CCAAGCAGTA CAATTTATGT CCACCAGTGC TGTGGTTCCC 
AGGTCTCAGA GGCAGAAGAA TCTCAGCTGG CTGGAAGAGA AAGAAAAAGA GGTGGTCAGT 
GCCTTGCGCT ACTTTAAGAC CATTGTGGAC AAAATGGCAA TTGACAAGAA GGTACTGGAA 
ATGCTTCCAG GTTCGGCCAG CAAGGTCCTG GAGGCCATCC TGCCCCTAGT GCAGACAGAC 
CCTCGCATTC AGCAGAGCTC AGCCATCTCC TCTTGCTACA GCCGGGTGTA TCAGAGTCTA 
GCCAATCTCA TTCGTTGGTC AGATCAAGTG ATGCTGGAAG GTGTGAACTC GGAAGATAAA 
GAGATGGTGA CAACTGTGAA AGAAGTCATC AAAGCTGTTT TGGATGGAGT GAAGGAGCTT 
GTCAGGCTCA CAATAGAGAA GCAGGAGCAT CCATCTCCCA CAAGTCCAGT TAAGCCCAGC 
TCACCAGCCT GCAAAACTGA CAGCCAACCT GAGCTCCCAT TGACTGACCG GGAAATGGAG 
ATTCTCAACA AGACAACTAG TATGTCCCAA TCAACGGAGC TGCTGCATCC TGACTCCATG 
GATGAAGAGG TTGCTCCTCC CAAGCCCCCC CTCCCTGGCA TCCGAGTGGT GGATAATAGT 
CCTCCACCAG CTTTACCACC CAAAAAGAGG CAGTCGGCAC CATCTCCCAC CAGAGTGGCT 
GTGGTTGCAC CCATGAGCCG GGCCACCAGT GGCTCAAGTT TACCTGTTGG AATCAATAGA 
CAGGACTTTG ATGTGGACTG TTATGCCCAA AGGAGGCTGT CTGGGGGCAG CCATTCATAT 
GGCGGGGAAT CCCCTCGCCT CTCCCCGTGC AGCAGCATCG GCAAGCTGAG CAAGTCCGAC 
GAACAGCTGT CCTCCTTGGA CCGGGACAGT GGCCAGTGCT CCAGGAATAC AAGCTGTGAA 
ACACTAGATC ACTATGACCC TGATTATGAA TTTCTACAGC AAGACCTCTC CACTGCTGAC 
CAGATACCTC AGCAAGTAGC ATGTAACCTT AGCCCATTGC CAGAATCTGT TGGTGAATCT 
GGCTCTCCTT TCCATGGACG TCCTTTCCAG CTGCCACCCA GCAACTCCCC ACAGCCAGAG 
CTTGGCAATC CCTTGCCCAT GGGGCATCCT ACGGACACAC CTCCTGCCCT GCCTGAGAAG 
AAGCGGAGGA GTGCAGCCTC TCAGACCACG GACAGCTCCA GCTCCAGCTG TAGAGTCTCC 
TATGAGAGGC ACCCTTCCCA ATATGACAAT ATCATGGAGG ATGATCTCCA GAACCCGGCC 
TCCCTCCAGC CAGGATCATT CACGCCATTC GCTGCTGTCC TCCCCTTCCA GCAAGGAGGC 
TCTTCAGCCA ATTTTGTGTC TGATTTTGCT GCTCCCGATT CAATGGGTGA CCCCGAGAAA 
CCACCTCCTC TACCTGAAAA GAAGAACAAA CACATGCTGG CCTACATGCA GCTCCTTGAG 
GACTACTCAG AGCCCCAGCC TTCCATGTTC TACCAGACGC CCCAGAACGA GCACATCTAC 
CAGCAGAAGA ACAAGCTCCT CATGGAGGTC TACGGCTTTA ATGACTCCTT CACCCAGGAG 
CTGGCCCCGC CCCCTGCCCT GCCCCCCAAG CAGAGGCAGC TGCAGGCCTC CTATGCTGCC 
TCTTCCTTCT CTTCTGTCTC CTACTGTGTC CAGCCAACTA AAGTGCCCTT CACGCCTGAG 
GACGCCAGTG CCACTCAGGG TCTCGGTATG TCCATCTCTA ACTCCTTTCT CAGCCGGCAC 
AGCTCCTTCC CTGTGCACTC GTATAAATCT GTGTTTAGGT CCTACTCCCA GGACTTCATG 
CCTCACAACC AAGCTTCCGT TCCACCTTTC CTCTCGTCTA CCTCCTCTTC CTCTCCACAT 
TTCCCACCTG TCCACCAGTC TCAGAGCTCT GACCTAGCAG TGCCAACCAC AGCCAGCCTG 
CCTCCCAGCA CTGTGGATGG GCCCAATTCC TGTTCTCAGG AGAGCAACTT GAATGGGAAT 
TCTGTCTACC TTCCTTCTGA AACCTCTTTC ACTGATTCTC TCCAGACGCC TTCGGAATCA 
CCAGCTACAA AAGATGGACA TCCCAAAGAT TCCTCTTCAC ATGGTGGGAA AGAAAACAGA 
GAAGGTGGTG AAAGGCAGCT GAAGTCCCCC GATGGTTTGG AATCGACCCA GTCAGAGGAG 
GAAGTGGATG AACTCTCTCT GATTGACCAC AATGAAATTA TGGCTAGGTT GACGTTAAAA 
CAAGAGGGTG ATGATGGGCC AGATGTCCGT GGGGGATCTG GGGATATATT GCTGGTCCAT 
GCAACTGAGA CCGATAGAAA AGACTTGGTT TTGTACTGTG AAGCCTTTTT GACAACCTAC 
AGGACATTTA TCACCCCTGA GGAACTCATT AAGAAGCTAC AGTATAGATA TGAGAAGTTC 
TGCCCCTTCC CAGACACATT CAAGAAGCGA GTCAGCAAGA ATACTTTCTT CGTGCTGGTG 
CGGGTAGTGG ATGAACTGTG CCTGGTGGAA TTAACTGAAG AGATCCTAAA GCTCTTGATG 
GAGCTGGTGT TCCGGTTAGT GTGCAGTGGG GAGCTGAGCC TTGCCCGGGT CCTCCGGAAG 
AATATCTTGG ACAAAGTGAA CCAAAAGAAG ATGCTAAAGT GTGCCAACTC GGACCAACCC 
CTGGCTGCTC GAGGGGTGGC TGCCAGGCCT GGGACTCTGC ATGACTTCCA CAGCCATGAG 
ATTGCTGAGC AGCTGACACT GTTGGATGCT GAGCTCTTCT ACAAGATAGA GATCCCAGAA 
GTTTTGCTTT GGGCAAAAGA GCAGAATGAA GAGAAGAGCC CTAACTTGAC ACAGTTTACT 
GAGCACTTTA ATAACATGTC CTACTGGGTT CGTTCAATAA TCATGTTGCA AGAAAAAGCC 
CAGGACAGAG AACGGCTGCT TCTGAAATTT ATCAAGATCA TGAAGCACTT GAGGAAGCTA 
AATAACTTCA ACTCGTATCT GGCTATCCTT TCAGCACTGG ACTCAGCACC CATCCGGAGG 
CTGGAGTGGC AAAAACAGAC CTCAGAGGGC CTGGCCGAGT ACTGCACTTT GATTGACAGC 
TCTTCCTCCT TCCGGGCCTA TCGGGCGGCT CTTTCTGAGG TGGAACCTCC ATGCATCCCA 
TACCTGGGTC TGATCCTGCA GGACCTCACC TTTGTTCACC TCGGAAACCC AGACTACATT 
GACGGGAAAG TGAATTTTTC CAAGCGCTGG CAGCAGTTCA ACATCCTGGA CAGTATGAGA 
TGTTTCCAGC AGGCACATTA TGAAATCCGC AGAAATGAGG ACATCGTGAA CTTCTTCAAT 
GATTTCAGTG ACCACCTAGC CGAGGAGGCC TTATGGGAAC TCTCTCTCAA AATCAAACCC 
CGGAACATAA CTCGGCGAAA AACAGATCGA GAAGAGAAGA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031810734.1
CDS	605..4309
Translation	MGNVNEKQKAWKQSNLYLWKQDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPADVPVKTFEKPVNKEATDNFLPEGYPPPLDLDHQAVQFMSTSAVVPRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQTDPRIQQSSAISSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKEVIKAVLDGVKELVRLTIEKQEHPSPTSPVKPSSPACKTDSQPELPLTDREMEILNKTTSMSQSTELLHPDSMDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSTADQIPQQVACNLSPLPESVGESGSPFHGRPFQLPPSNSPQPELGNPLPMGHPTDTPPALPEKKRRSAASQTTDSSSSSCRVSYERHPSQYDNIMEDDLQNPASLQPGSFTPFAAVLPFQQGGSSANFVSDFAAPDSMGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFNDSFTQELAPPPALPPKQRQLQASYAASSFSSVSYCVQPTKVPFTPEDASATQGLGMSISNSFLSRHSSFPVHSYKSVFRSYSQDFMPHNQASVPPFLSSTSSSSPHFPPVHQSQSSDLAVPTTASLPPSTVDGPNSCSQESNLNGNSVYLPSETSFTDSLQTPSESPATKDGHPKDSSSHGGKENREGGERQLKSPDGLESTQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIKKLQYRYEKFCPFPDTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCSGELSLARVLRKNILDKVNQKKMLKCANSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYEIRRNEDIVNFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_031954874.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031954874.1

ORF Insert Sequence:

ATGGGGAATG TTAATGAAAA ACAAAAAGCT TGGAAGCAGA GCAATTTATA CCTCTGGAAA 
CAAGATTCCC AGCGCTCTCA TCTCTCCTCC TTCACCATGA AGCTGATGGA CAAATTTCAC 
TCGCCCAAAA TCAAGAGAAC ACCATCAAAG AAAGGAAAAC CAGCCGATGT GCCTGTGAAA 
ACTTTTGAGA AACCTGTGAA CAAAGAGGCC ACAGACAATT TTCTACCAGA GGGCTACCCT 
CCTCCCTTGG ATCTGGATCA CCAAGCAGTA CAATTTATGT CCACCAGTGC TGTGGTTCCC 
AGGTCTCAGA GGCAGAAGAA TCTCAGCTGG CTGGAAGAGA AAGAAAAAGA GGTGGTCAGT 
GCCTTGCGCT ACTTTAAGAC CATTGTGGAC AAAATGGCAA TTGACAAGAA GGTACTGGAA 
ATGCTTCCAG GTTCGGCCAG CAAGGTCCTG GAGGCCATCC TGCCCCTAGT GCAGACAGAC 
CCTCGCATTC AGCAGAGCTC AGCCATCTCC TCTTGCTACA GCCGGGTGTA TCAGAGTCTA 
GCCAATCTCA TTCGTTGGTC AGATCAAGTG ATGCTGGAAG GTGTGAACTC GGAAGATAAA 
GAGATGGTGA CAACTGTGAA AGAAGTCATC AAAGCTGTTT TGGATGGAGT GAAGGAGCTT 
GTCAGGCTCA CAATAGAGAA GCAGGAGCAT CCATCTCCCA CAAGTCCAGT TAAGCCCAGC 
TCACCAGCCT GCAAAACTGA CAGCCAACCT GAGCTCCCAT TGACTGACCG GGAAATGGAG 
ATTCTCAACA AGACAACTAG TATGTCCCAA TCAACGGAGC TGCTGCATCC TGACTCCATG 
GATGAAGAGG TTGCTCCTCC CAAGCCCCCC CTCCCTGGCA TCCGAGTGGT GGATAATAGT 
CCTCCACCAG CTTTACCACC CAAAAAGAGG CAGTCGGCAC CATCTCCCAC CAGAGTGGCT 
GTGGTTGCAC CCATGAGCCG GGCCACCAGT GGCTCAAGTT TACCTGTTGG AATCAATAGA 
CAGGACTTTG ATGTGGACTG TTATGCCCAA AGGAGGCTGT CTGGGGGCAG CCATTCATAT 
GGCGGGGAAT CCCCTCGCCT CTCCCCGTGC AGCAGCATCG GCAAGCTGAG CAAGTCCGAC 
GAACAGCTGT CCTCCTTGGA CCGGGACAGT GGCCAGTGCT CCAGGAATAC AAGCTGTGAA 
ACACTAGATC ACTATGACCC TGATTATGAA TTTCTACAGC AAGACCTCTC CACTGCTGAC 
CAGATACCTC AGCAAGTAGC ATGTAACCTT AGCCCATTGC CAGAATCTGT TGGTGAATCT 
GGCTCTCCTT TCCATGGACG TCCTTTCCAG CTGCCACCCA GCAACTCCCC ACAGCCAGAG 
CTTGGCAATC CCTTGCCCAT GGGGCATCCT ACGGACACAC CTCCTGCCCT GCCTGAGAAG 
AAGCGGAGGA GTGCAGCCTC TCAGACCACG GACAGCTCCA GCTCCAGCTG TAGAGTCTCC 
TATGAGAGGC ACCCTTCCCA ATATGACAAT ATCATGGAGG ATGATCTCCA GAACCCGGCC 
TCCCTCCAGC CAGGATCATT CACGCCATTC GCTGCTGTCC TCCCCTTCCA GCAAGGAGGC 
TCTTCAGCCA ATTTTGTGTC TGATTTTGCT GCTCCCGATT CAATGGGTGA CCCCGAGAAA 
CCACCTCCTC TACCTGAAAA GAAGAACAAA CACATGCTGG CCTACATGCA GCTCCTTGAG 
GACTACTCAG AGCCCCAGCC TTCCATGTTC TACCAGACGC CCCAGAACGA GCACATCTAC 
CAGCAGAAGA ACAAGCTCCT CATGGAGGTC TACGGCTTTA ATGACTCCTT CACCCAGGAG 
CTGGCCCCGC CCCCTGCCCT GCCCCCCAAG CAGAGGCAGC TGCAGGCCTC CTATGCTGCC 
TCTTCCTTCT CTTCTGTCTC CTACTGTGTC CAGCCAACTA AAGTGCCCTT CACGCCTGAG 
GACGCCAGTG CCACTCAGGG TCTCGGTATG TCCATCTCTA ACTCCTTTCT CAGCCGGCAC 
AGCTCCTTCC CTGTGCACTC GTATAAATCT GTGTTTAGGT CCTACTCCCA GGACTTCATG 
CCTCACAACC AAGCTTCCGT TCCACCTTTC CTCTCGTCTA CCTCCTCTTC CTCTCCACAT 
TTCCCACCTG TCCACCAGTC TCAGAGCTCT GACCTAGCAG TGCCAACCAC AGCCAGCCTG 
CCTCCCAGCA CTGTGGATGG GCCCAATTCC TGTTCTCAGG AGAGCAACTT GAATGGGAAT 
TCTGTCTACC TTCCTTCTGA AACCTCTTTC ACTGATTCTC TCCAGACGCC TTCGGAATCA 
CCAGCTACAA AAGATGGACA TCCCAAAGAT TCCTCTTCAC ATGGTGGGAA AGAAAACAGA 
GAAGGTGGTG AAAGGCAGCT GAAGTCCCCC GATGGTTTGG AATCGACCCA GTCAGAGGAG 
GAAGTGGATG AACTCTCTCT GATTGACCAC AATGAAATTA TGGCTAGGTT GACGTTAAAA 
CAAGAGGGTG ATGATGGGCC AGATGTCCGT GGGGGATCTG GGGATATATT GCTGGTCCAT 
GCAACTGAGA CCGATAGAAA AGACTTGGTT TTGTACTGTG AAGCCTTTTT GACAACCTAC 
AGGACATTTA TCACCCCTGA GGAACTCATT AAGAAGCTAC AGTATAGATA TGAGAAGTTC 
TGCCCCTTCC CAGACACATT CAAGAAGCGA GTCAGCAAGA ATACTTTCTT CGTGCTGGTG 
CGGGTAGTGG ATGAACTGTG CCTGGTGGAA TTAACTGAAG AGATCCTAAA GCTCTTGATG 
GAGCTGGTGT TCCGGTTAGT GTGCAGTGGG GAGCTGAGCC TTGCCCGGGT CCTCCGGAAG 
AATATCTTGG ACAAAGTGAA CCAAAAGAAG ATGCTAAAGT GTGCCAACTC GGACCAACCC 
CTGGCTGCTC GAGGGGTGGC TGCCAGGCCT GGGACTCTGC ATGACTTCCA CAGCCATGAG 
ATTGCTGAGC AGCTGACACT GTTGGATGCT GAGCTCTTCT ACAAGATAGA GATCCCAGAA 
GTTTTGCTTT GGGCAAAAGA GCAGAATGAA GAGAAGAGCC CTAACTTGAC ACAGTTTACT 
GAGCACTTTA ATAACATGTC CTACTGGGTT CGTTCAATAA TCATGTTGCA AGAAAAAGCC 
CAGGACAGAG AACGGCTGCT TCTGAAATTT ATCAAGATCA TGAAGCACTT GAGGAAGCTA 
AATAACTTCA ACTCGTATCT GGCTATCCTT TCAGCACTGG ACTCAGCACC CATCCGGAGG 
CTGGAGTGGC AAAAACAGAC CTCAGAGGGC CTGGCCGAGT ACTGCACTTT GATTGACAGC 
TCTTCCTCCT TCCGGGCCTA TCGGGCGGCT CTTTCTGAGG TGGAACCTCC ATGCATCCCA 
TACCTGGGTC TGATCCTGCA GGACCTCACC TTTGTTCACC TCGGAAACCC AGACTACATT 
GACGGGAAAG TGAATTTTTC CAAGCGCTGG CAGCAGTTCA ACATCCTGGA CAGTATGAGA 
TGTTTCCAGC AGGCACATTA TGAAATCCGC AGAAATGAGG ACATCGTGAA CTTCTTCAAT 
GATTTCAGTG ACCACCTAGC CGAGGAGGCC TTATGGGAAC TCTCTCTCAA AATCAAACCC 
CGGAACATAA CTCGGCGAAA AACAGATCGA GAAGAGAAGA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_031954875.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031810735.1 Sarcophilus harrisii Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSa226539
Clone ID Related Accession (Same CDS sequence)	XM_031954875.1
Accession Version	XM_031954875.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3666bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	rap guanine nucleotide exchange factor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045427.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCA AGATCGAGAA AGCAGATTCC CAGCGCTCTC ATCTCTCCTC CTTCACCATG 
AAGCTGATGG ACAAATTTCA CTCGCCCAAA ATCAAGAGAA CACCATCAAA GAAAGGAAAA 
CCAGCCGATG TGCCTGTGAA AACTTTTGAG AAACCTGTGA ACAAAGAGGC CACAGACAAT 
TTTCTACCAG AGGGCTACCC TCCTCCCTTG GATCTGGATC ACCAAGCAGT ACAATTTATG 
TCCACCAGTG CTGTGGTTCC CAGGTCTCAG AGGCAGAAGA ATCTCAGCTG GCTGGAAGAG 
AAAGAAAAAG AGGTGGTCAG TGCCTTGCGC TACTTTAAGA CCATTGTGGA CAAAATGGCA 
ATTGACAAGA AGGTACTGGA AATGCTTCCA GGTTCGGCCA GCAAGGTCCT GGAGGCCATC 
CTGCCCCTAG TGCAGACAGA CCCTCGCATT CAGCAGAGCT CAGCCATCTC CTCTTGCTAC 
AGCCGGGTGT ATCAGAGTCT AGCCAATCTC ATTCGTTGGT CAGATCAAGT GATGCTGGAA 
GGTGTGAACT CGGAAGATAA AGAGATGGTG ACAACTGTGA AAGAAGTCAT CAAAGCTGTT 
TTGGATGGAG TGAAGGAGCT TGTCAGGCTC ACAATAGAGA AGCAGGAGCA TCCATCTCCC 
ACAAGTCCAG TTAAGCCCAG CTCACCAGCC TGCAAAACTG ACAGCCAACC TGAGCTCCCA 
TTGACTGACC GGGAAATGGA GATTCTCAAC AAGACAACTA GTATGTCCCA ATCAACGGAG 
CTGCTGCATC CTGACTCCAT GGATGAAGAG GTTGCTCCTC CCAAGCCCCC CCTCCCTGGC 
ATCCGAGTGG TGGATAATAG TCCTCCACCA GCTTTACCAC CCAAAAAGAG GCAGTCGGCA 
CCATCTCCCA CCAGAGTGGC TGTGGTTGCA CCCATGAGCC GGGCCACCAG TGGCTCAAGT 
TTACCTGTTG GAATCAATAG ACAGGACTTT GATGTGGACT GTTATGCCCA AAGGAGGCTG 
TCTGGGGGCA GCCATTCATA TGGCGGGGAA TCCCCTCGCC TCTCCCCGTG CAGCAGCATC 
GGCAAGCTGA GCAAGTCCGA CGAACAGCTG TCCTCCTTGG ACCGGGACAG TGGCCAGTGC 
TCCAGGAATA CAAGCTGTGA AACACTAGAT CACTATGACC CTGATTATGA ATTTCTACAG 
CAAGACCTCT CCACTGCTGA CCAGATACCT CAGCAAGTAG CATGTAACCT TAGCCCATTG 
CCAGAATCTG TTGGTGAATC TGGCTCTCCT TTCCATGGAC GTCCTTTCCA GCTGCCACCC 
AGCAACTCCC CACAGCCAGA GCTTGGCAAT CCCTTGCCCA TGGGGCATCC TACGGACACA 
CCTCCTGCCC TGCCTGAGAA GAAGCGGAGG AGTGCAGCCT CTCAGACCAC GGACAGCTCC 
AGCTCCAGCT GTAGAGTCTC CTATGAGAGG CACCCTTCCC AATATGACAA TATCATGGAG 
GATGATCTCC AGAACCCGGC CTCCCTCCAG CCAGGATCAT TCACGCCATT CGCTGCTGTC 
CTCCCCTTCC AGCAAGGAGG CTCTTCAGCC AATTTTGTGT CTGATTTTGC TGCTCCCGAT 
TCAATGGGTG ACCCCGAGAA ACCACCTCCT CTACCTGAAA AGAAGAACAA ACACATGCTG 
GCCTACATGC AGCTCCTTGA GGACTACTCA GAGCCCCAGC CTTCCATGTT CTACCAGACG 
CCCCAGAACG AGCACATCTA CCAGCAGAAG AACAAGCTCC TCATGGAGGT CTACGGCTTT 
AATGACTCCT TCACCCAGGA GCTGGCCCCG CCCCCTGCCC TGCCCCCCAA GCAGAGGCAG 
CTGCAGGCCT CCTATGCTGC CTCTTCCTTC TCTTCTGTCT CCTACTGTGT CCAGCCAACT 
AAAGTGCCCT TCACGCCTGA GGACGCCAGT GCCACTCAGG GTCTCGGTAT GTCCATCTCT 
AACTCCTTTC TCAGCCGGCA CAGCTCCTTC CCTGTGCACT CGTATAAATC TGTGTTTAGG 
TCCTACTCCC AGGACTTCAT GCCTCACAAC CAAGCTTCCG TTCCACCTTT CCTCTCGTCT 
ACCTCCTCTT CCTCTCCACA TTTCCCACCT GTCCACCAGT CTCAGAGCTC TGACCTAGCA 
GTGCCAACCA CAGCCAGCCT GCCTCCCAGC ACTGTGGATG GGCCCAATTC CTGTTCTCAG 
GAGAGCAACT TGAATGGGAA TTCTGTCTAC CTTCCTTCTG AAACCTCTTT CACTGATTCT 
CTCCAGACGC CTTCGGAATC ACCAGCTACA AAAGATGGAC ATCCCAAAGA TTCCTCTTCA 
CATGGTGGGA AAGAAAACAG AGAAGGTGGT GAAAGGCAGC TGAAGTCCCC CGATGGTTTG 
GAATCGACCC AGTCAGAGGA GGAAGTGGAT GAACTCTCTC TGATTGACCA CAATGAAATT 
ATGGCTAGGT TGACGTTAAA ACAAGAGGGT GATGATGGGC CAGATGTCCG TGGGGGATCT 
GGGGATATAT TGCTGGTCCA TGCAACTGAG ACCGATAGAA AAGACTTGGT TTTGTACTGT 
GAAGCCTTTT TGACAACCTA CAGGACATTT ATCACCCCTG AGGAACTCAT TAAGAAGCTA 
CAGTATAGAT ATGAGAAGTT CTGCCCCTTC CCAGACACAT TCAAGAAGCG AGTCAGCAAG 
AATACTTTCT TCGTGCTGGT GCGGGTAGTG GATGAACTGT GCCTGGTGGA ATTAACTGAA 
GAGATCCTAA AGCTCTTGAT GGAGCTGGTG TTCCGGTTAG TGTGCAGTGG GGAGCTGAGC 
CTTGCCCGGG TCCTCCGGAA GAATATCTTG GACAAAGTGA ACCAAAAGAA GATGCTAAAG 
TGTGCCAACT CGGACCAACC CCTGGCTGCT CGAGGGGTGG CTGCCAGGCC TGGGACTCTG 
CATGACTTCC ACAGCCATGA GATTGCTGAG CAGCTGACAC TGTTGGATGC TGAGCTCTTC 
TACAAGATAG AGATCCCAGA AGTTTTGCTT TGGGCAAAAG AGCAGAATGA AGAGAAGAGC 
CCTAACTTGA CACAGTTTAC TGAGCACTTT AATAACATGT CCTACTGGGT TCGTTCAATA 
ATCATGTTGC AAGAAAAAGC CCAGGACAGA GAACGGCTGC TTCTGAAATT TATCAAGATC 
ATGAAGCACT TGAGGAAGCT AAATAACTTC AACTCGTATC TGGCTATCCT TTCAGCACTG 
GACTCAGCAC CCATCCGGAG GCTGGAGTGG CAAAAACAGA CCTCAGAGGG CCTGGCCGAG 
TACTGCACTT TGATTGACAG CTCTTCCTCC TTCCGGGCCT ATCGGGCGGC TCTTTCTGAG 
GTGGAACCTC CATGCATCCC ATACCTGGGT CTGATCCTGC AGGACCTCAC CTTTGTTCAC 
CTCGGAAACC CAGACTACAT TGACGGGAAA GTGAATTTTT CCAAGCGCTG GCAGCAGTTC 
AACATCCTGG ACAGTATGAG ATGTTTCCAG CAGGCACATT ATGAAATCCG CAGAAATGAG 
GACATCGTGA ACTTCTTCAA TGATTTCAGT GACCACCTAG CCGAGGAGGC CTTATGGGAA 
CTCTCTCTCA AAATCAAACC CCGGAACATA ACTCGGCGAA AAACAGATCG AGAAGAGAAG 
ACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031810735.1
CDS	269..3934
Translation	MSGKIEKADSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPADVPVKTFEKPVNKEATDNFLPEGYPPPLDLDHQAVQFMSTSAVVPRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQTDPRIQQSSAISSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKEVIKAVLDGVKELVRLTIEKQEHPSPTSPVKPSSPACKTDSQPELPLTDREMEILNKTTSMSQSTELLHPDSMDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSTADQIPQQVACNLSPLPESVGESGSPFHGRPFQLPPSNSPQPELGNPLPMGHPTDTPPALPEKKRRSAASQTTDSSSSSCRVSYERHPSQYDNIMEDDLQNPASLQPGSFTPFAAVLPFQQGGSSANFVSDFAAPDSMGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFNDSFTQELAPPPALPPKQRQLQASYAASSFSSVSYCVQPTKVPFTPEDASATQGLGMSISNSFLSRHSSFPVHSYKSVFRSYSQDFMPHNQASVPPFLSSTSSSSPHFPPVHQSQSSDLAVPTTASLPPSTVDGPNSCSQESNLNGNSVYLPSETSFTDSLQTPSESPATKDGHPKDSSSHGGKENREGGERQLKSPDGLESTQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIKKLQYRYEKFCPFPDTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCSGELSLARVLRKNILDKVNQKKMLKCANSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYEIRRNEDIVNFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_031954875.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031954875.1

ORF Insert Sequence:

ATGTCTGGCA AGATCGAGAA AGCAGATTCC CAGCGCTCTC ATCTCTCCTC CTTCACCATG 
AAGCTGATGG ACAAATTTCA CTCGCCCAAA ATCAAGAGAA CACCATCAAA GAAAGGAAAA 
CCAGCCGATG TGCCTGTGAA AACTTTTGAG AAACCTGTGA ACAAAGAGGC CACAGACAAT 
TTTCTACCAG AGGGCTACCC TCCTCCCTTG GATCTGGATC ACCAAGCAGT ACAATTTATG 
TCCACCAGTG CTGTGGTTCC CAGGTCTCAG AGGCAGAAGA ATCTCAGCTG GCTGGAAGAG 
AAAGAAAAAG AGGTGGTCAG TGCCTTGCGC TACTTTAAGA CCATTGTGGA CAAAATGGCA 
ATTGACAAGA AGGTACTGGA AATGCTTCCA GGTTCGGCCA GCAAGGTCCT GGAGGCCATC 
CTGCCCCTAG TGCAGACAGA CCCTCGCATT CAGCAGAGCT CAGCCATCTC CTCTTGCTAC 
AGCCGGGTGT ATCAGAGTCT AGCCAATCTC ATTCGTTGGT CAGATCAAGT GATGCTGGAA 
GGTGTGAACT CGGAAGATAA AGAGATGGTG ACAACTGTGA AAGAAGTCAT CAAAGCTGTT 
TTGGATGGAG TGAAGGAGCT TGTCAGGCTC ACAATAGAGA AGCAGGAGCA TCCATCTCCC 
ACAAGTCCAG TTAAGCCCAG CTCACCAGCC TGCAAAACTG ACAGCCAACC TGAGCTCCCA 
TTGACTGACC GGGAAATGGA GATTCTCAAC AAGACAACTA GTATGTCCCA ATCAACGGAG 
CTGCTGCATC CTGACTCCAT GGATGAAGAG GTTGCTCCTC CCAAGCCCCC CCTCCCTGGC 
ATCCGAGTGG TGGATAATAG TCCTCCACCA GCTTTACCAC CCAAAAAGAG GCAGTCGGCA 
CCATCTCCCA CCAGAGTGGC TGTGGTTGCA CCCATGAGCC GGGCCACCAG TGGCTCAAGT 
TTACCTGTTG GAATCAATAG ACAGGACTTT GATGTGGACT GTTATGCCCA AAGGAGGCTG 
TCTGGGGGCA GCCATTCATA TGGCGGGGAA TCCCCTCGCC TCTCCCCGTG CAGCAGCATC 
GGCAAGCTGA GCAAGTCCGA CGAACAGCTG TCCTCCTTGG ACCGGGACAG TGGCCAGTGC 
TCCAGGAATA CAAGCTGTGA AACACTAGAT CACTATGACC CTGATTATGA ATTTCTACAG 
CAAGACCTCT CCACTGCTGA CCAGATACCT CAGCAAGTAG CATGTAACCT TAGCCCATTG 
CCAGAATCTG TTGGTGAATC TGGCTCTCCT TTCCATGGAC GTCCTTTCCA GCTGCCACCC 
AGCAACTCCC CACAGCCAGA GCTTGGCAAT CCCTTGCCCA TGGGGCATCC TACGGACACA 
CCTCCTGCCC TGCCTGAGAA GAAGCGGAGG AGTGCAGCCT CTCAGACCAC GGACAGCTCC 
AGCTCCAGCT GTAGAGTCTC CTATGAGAGG CACCCTTCCC AATATGACAA TATCATGGAG 
GATGATCTCC AGAACCCGGC CTCCCTCCAG CCAGGATCAT TCACGCCATT CGCTGCTGTC 
CTCCCCTTCC AGCAAGGAGG CTCTTCAGCC AATTTTGTGT CTGATTTTGC TGCTCCCGAT 
TCAATGGGTG ACCCCGAGAA ACCACCTCCT CTACCTGAAA AGAAGAACAA ACACATGCTG 
GCCTACATGC AGCTCCTTGA GGACTACTCA GAGCCCCAGC CTTCCATGTT CTACCAGACG 
CCCCAGAACG AGCACATCTA CCAGCAGAAG AACAAGCTCC TCATGGAGGT CTACGGCTTT 
AATGACTCCT TCACCCAGGA GCTGGCCCCG CCCCCTGCCC TGCCCCCCAA GCAGAGGCAG 
CTGCAGGCCT CCTATGCTGC CTCTTCCTTC TCTTCTGTCT CCTACTGTGT CCAGCCAACT 
AAAGTGCCCT TCACGCCTGA GGACGCCAGT GCCACTCAGG GTCTCGGTAT GTCCATCTCT 
AACTCCTTTC TCAGCCGGCA CAGCTCCTTC CCTGTGCACT CGTATAAATC TGTGTTTAGG 
TCCTACTCCC AGGACTTCAT GCCTCACAAC CAAGCTTCCG TTCCACCTTT CCTCTCGTCT 
ACCTCCTCTT CCTCTCCACA TTTCCCACCT GTCCACCAGT CTCAGAGCTC TGACCTAGCA 
GTGCCAACCA CAGCCAGCCT GCCTCCCAGC ACTGTGGATG GGCCCAATTC CTGTTCTCAG 
GAGAGCAACT TGAATGGGAA TTCTGTCTAC CTTCCTTCTG AAACCTCTTT CACTGATTCT 
CTCCAGACGC CTTCGGAATC ACCAGCTACA AAAGATGGAC ATCCCAAAGA TTCCTCTTCA 
CATGGTGGGA AAGAAAACAG AGAAGGTGGT GAAAGGCAGC TGAAGTCCCC CGATGGTTTG 
GAATCGACCC AGTCAGAGGA GGAAGTGGAT GAACTCTCTC TGATTGACCA CAATGAAATT 
ATGGCTAGGT TGACGTTAAA ACAAGAGGGT GATGATGGGC CAGATGTCCG TGGGGGATCT 
GGGGATATAT TGCTGGTCCA TGCAACTGAG ACCGATAGAA AAGACTTGGT TTTGTACTGT 
GAAGCCTTTT TGACAACCTA CAGGACATTT ATCACCCCTG AGGAACTCAT TAAGAAGCTA 
CAGTATAGAT ATGAGAAGTT CTGCCCCTTC CCAGACACAT TCAAGAAGCG AGTCAGCAAG 
AATACTTTCT TCGTGCTGGT GCGGGTAGTG GATGAACTGT GCCTGGTGGA ATTAACTGAA 
GAGATCCTAA AGCTCTTGAT GGAGCTGGTG TTCCGGTTAG TGTGCAGTGG GGAGCTGAGC 
CTTGCCCGGG TCCTCCGGAA GAATATCTTG GACAAAGTGA ACCAAAAGAA GATGCTAAAG 
TGTGCCAACT CGGACCAACC CCTGGCTGCT CGAGGGGTGG CTGCCAGGCC TGGGACTCTG 
CATGACTTCC ACAGCCATGA GATTGCTGAG CAGCTGACAC TGTTGGATGC TGAGCTCTTC 
TACAAGATAG AGATCCCAGA AGTTTTGCTT TGGGCAAAAG AGCAGAATGA AGAGAAGAGC 
CCTAACTTGA CACAGTTTAC TGAGCACTTT AATAACATGT CCTACTGGGT TCGTTCAATA 
ATCATGTTGC AAGAAAAAGC CCAGGACAGA GAACGGCTGC TTCTGAAATT TATCAAGATC 
ATGAAGCACT TGAGGAAGCT AAATAACTTC AACTCGTATC TGGCTATCCT TTCAGCACTG 
GACTCAGCAC CCATCCGGAG GCTGGAGTGG CAAAAACAGA CCTCAGAGGG CCTGGCCGAG 
TACTGCACTT TGATTGACAG CTCTTCCTCC TTCCGGGCCT ATCGGGCGGC TCTTTCTGAG 
GTGGAACCTC CATGCATCCC ATACCTGGGT CTGATCCTGC AGGACCTCAC CTTTGTTCAC 
CTCGGAAACC CAGACTACAT TGACGGGAAA GTGAATTTTT CCAAGCGCTG GCAGCAGTTC 
AACATCCTGG ACAGTATGAG ATGTTTCCAG CAGGCACATT ATGAAATCCG CAGAAATGAG 
GACATCGTGA ACTTCTTCAA TGATTTCAGT GACCACCTAG CCGAGGAGGC CTTATGGGAA 
CTCTCTCTCA AAATCAAACC CCGGAACATA ACTCGGCGAA AAACAGATCG AGAAGAGAAG 
ACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_031954876.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031810736.1 Sarcophilus harrisii Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OSa226540
Clone ID Related Accession (Same CDS sequence)	XM_031954876.1
Accession Version	XM_031954876.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3591bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	rap guanine nucleotide exchange factor 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045427.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAATG TTAATGAAAA ACAAAAAGCT TGGAAGCAGA GCAATTTATA CCTCTGGAAA 
CAAGATTCCC AGCGCTCTCA TCTCTCCTCC TTCACCATGA AGCTGATGGA CAAATTTCAC 
TCGCCCAAAA TCAAGAGAAC ACCATCAAAG AAAGGAAAAC CAGCCGATGT GCCTGTGAAA 
ACTTTTGAGA AACCTGTGAA CAAAAATCTC AGCTGGCTGG AAGAGAAAGA AAAAGAGGTG 
GTCAGTGCCT TGCGCTACTT TAAGACCATT GTGGACAAAA TGGCAATTGA CAAGAAGGTA 
CTGGAAATGC TTCCAGGTTC GGCCAGCAAG GTCCTGGAGG CCATCCTGCC CCTAGTGCAG 
ACAGACCCTC GCATTCAGCA GAGCTCAGCC ATCTCCTCTT GCTACAGCCG GGTGTATCAG 
AGTCTAGCCA ATCTCATTCG TTGGTCAGAT CAAGTGATGC TGGAAGGTGT GAACTCGGAA 
GATAAAGAGA TGGTGACAAC TGTGAAAGAA GTCATCAAAG CTGTTTTGGA TGGAGTGAAG 
GAGCTTGTCA GGCTCACAAT AGAGAAGCAG GAGCATCCAT CTCCCACAAG TCCAGTTAAG 
CCCAGCTCAC CAGCCTGCAA AACTGACAGC CAACCTGAGC TCCCATTGAC TGACCGGGAA 
ATGGAGATTC TCAACAAGAC AACTAGTATG TCCCAATCAA CGGAGCTGCT GCATCCTGAC 
TCCATGGATG AAGAGGTTGC TCCTCCCAAG CCCCCCCTCC CTGGCATCCG AGTGGTGGAT 
AATAGTCCTC CACCAGCTTT ACCACCCAAA AAGAGGCAGT CGGCACCATC TCCCACCAGA 
GTGGCTGTGG TTGCACCCAT GAGCCGGGCC ACCAGTGGCT CAAGTTTACC TGTTGGAATC 
AATAGACAGG ACTTTGATGT GGACTGTTAT GCCCAAAGGA GGCTGTCTGG GGGCAGCCAT 
TCATATGGCG GGGAATCCCC TCGCCTCTCC CCGTGCAGCA GCATCGGCAA GCTGAGCAAG 
TCCGACGAAC AGCTGTCCTC CTTGGACCGG GACAGTGGCC AGTGCTCCAG GAATACAAGC 
TGTGAAACAC TAGATCACTA TGACCCTGAT TATGAATTTC TACAGCAAGA CCTCTCCACT 
GCTGACCAGA TACCTCAGCA AGTAGCATGT AACCTTAGCC CATTGCCAGA ATCTGTTGGT 
GAATCTGGCT CTCCTTTCCA TGGACGTCCT TTCCAGCTGC CACCCAGCAA CTCCCCACAG 
CCAGAGCTTG GCAATCCCTT GCCCATGGGG CATCCTACGG ACACACCTCC TGCCCTGCCT 
GAGAAGAAGC GGAGGAGTGC AGCCTCTCAG ACCACGGACA GCTCCAGCTC CAGCTGTAGA 
GTCTCCTATG AGAGGCACCC TTCCCAATAT GACAATATCA TGGAGGATGA TCTCCAGAAC 
CCGGCCTCCC TCCAGCCAGG ATCATTCACG CCATTCGCTG CTGTCCTCCC CTTCCAGCAA 
GGAGGCTCTT CAGCCAATTT TGTGTCTGAT TTTGCTGCTC CCGATTCAAT GGGTGACCCC 
GAGAAACCAC CTCCTCTACC TGAAAAGAAG AACAAACACA TGCTGGCCTA CATGCAGCTC 
CTTGAGGACT ACTCAGAGCC CCAGCCTTCC ATGTTCTACC AGACGCCCCA GAACGAGCAC 
ATCTACCAGC AGAAGAACAA GCTCCTCATG GAGGTCTACG GCTTTAATGA CTCCTTCACC 
CAGGAGCTGG CCCCGCCCCC TGCCCTGCCC CCCAAGCAGA GGCAGCTGCA GGCCTCCTAT 
GCTGCCTCTT CCTTCTCTTC TGTCTCCTAC TGTGTCCAGC CAACTAAAGT GCCCTTCACG 
CCTGAGGACG CCAGTGCCAC TCAGGGTCTC GGTATGTCCA TCTCTAACTC CTTTCTCAGC 
CGGCACAGCT CCTTCCCTGT GCACTCGTAT AAATCTGTGT TTAGGTCCTA CTCCCAGGAC 
TTCATGCCTC ACAACCAAGC TTCCGTTCCA CCTTTCCTCT CGTCTACCTC CTCTTCCTCT 
CCACATTTCC CACCTGTCCA CCAGTCTCAG AGCTCTGACC TAGCAGTGCC AACCACAGCC 
AGCCTGCCTC CCAGCACTGT GGATGGGCCC AATTCCTGTT CTCAGGAGAG CAACTTGAAT 
GGGAATTCTG TCTACCTTCC TTCTGAAACC TCTTTCACTG ATTCTCTCCA GACGCCTTCG 
GAATCACCAG CTACAAAAGA TGGACATCCC AAAGATTCCT CTTCACATGG TGGGAAAGAA 
AACAGAGAAG GTGGTGAAAG GCAGCTGAAG TCCCCCGATG GTTTGGAATC GACCCAGTCA 
GAGGAGGAAG TGGATGAACT CTCTCTGATT GACCACAATG AAATTATGGC TAGGTTGACG 
TTAAAACAAG AGGGTGATGA TGGGCCAGAT GTCCGTGGGG GATCTGGGGA TATATTGCTG 
GTCCATGCAA CTGAGACCGA TAGAAAAGAC TTGGTTTTGT ACTGTGAAGC CTTTTTGACA 
ACCTACAGGA CATTTATCAC CCCTGAGGAA CTCATTAAGA AGCTACAGTA TAGATATGAG 
AAGTTCTGCC CCTTCCCAGA CACATTCAAG AAGCGAGTCA GCAAGAATAC TTTCTTCGTG 
CTGGTGCGGG TAGTGGATGA ACTGTGCCTG GTGGAATTAA CTGAAGAGAT CCTAAAGCTC 
TTGATGGAGC TGGTGTTCCG GTTAGTGTGC AGTGGGGAGC TGAGCCTTGC CCGGGTCCTC 
CGGAAGAATA TCTTGGACAA AGTGAACCAA AAGAAGATGC TAAAGTGTGC CAACTCGGAC 
CAACCCCTGG CTGCTCGAGG GGTGGCTGCC AGGCCTGGGA CTCTGCATGA CTTCCACAGC 
CATGAGATTG CTGAGCAGCT GACACTGTTG GATGCTGAGC TCTTCTACAA GATAGAGATC 
CCAGAAGTTT TGCTTTGGGC AAAAGAGCAG AATGAAGAGA AGAGCCCTAA CTTGACACAG 
TTTACTGAGC ACTTTAATAA CATGTCCTAC TGGGTTCGTT CAATAATCAT GTTGCAAGAA 
AAAGCCCAGG ACAGAGAACG GCTGCTTCTG AAATTTATCA AGATCATGAA GCACTTGAGG 
AAGCTAAATA ACTTCAACTC GTATCTGGCT ATCCTTTCAG CACTGGACTC AGCACCCATC 
CGGAGGCTGG AGTGGCAAAA ACAGACCTCA GAGGGCCTGG CCGAGTACTG CACTTTGATT 
GACAGCTCTT CCTCCTTCCG GGCCTATCGG GCGGCTCTTT CTGAGGTGGA ACCTCCATGC 
ATCCCATACC TGGGTCTGAT CCTGCAGGAC CTCACCTTTG TTCACCTCGG AAACCCAGAC 
TACATTGACG GGAAAGTGAA TTTTTCCAAG CGCTGGCAGC AGTTCAACAT CCTGGACAGT 
ATGAGATGTT TCCAGCAGGC ACATTATGAA ATCCGCAGAA ATGAGGACAT CGTGAACTTC 
TTCAATGATT TCAGTGACCA CCTAGCCGAG GAGGCCTTAT GGGAACTCTC TCTCAAAATC 
AAACCCCGGA ACATAACTCG GCGAAAAACA GATCGAGAAG AGAAGACCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031810736.1
CDS	605..4195
Translation	MGNVNEKQKAWKQSNLYLWKQDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPADVPVKTFEKPVNKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQTDPRIQQSSAISSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKEVIKAVLDGVKELVRLTIEKQEHPSPTSPVKPSSPACKTDSQPELPLTDREMEILNKTTSMSQSTELLHPDSMDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSTADQIPQQVACNLSPLPESVGESGSPFHGRPFQLPPSNSPQPELGNPLPMGHPTDTPPALPEKKRRSAASQTTDSSSSSCRVSYERHPSQYDNIMEDDLQNPASLQPGSFTPFAAVLPFQQGGSSANFVSDFAAPDSMGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFNDSFTQELAPPPALPPKQRQLQASYAASSFSSVSYCVQPTKVPFTPEDASATQGLGMSISNSFLSRHSSFPVHSYKSVFRSYSQDFMPHNQASVPPFLSSTSSSSPHFPPVHQSQSSDLAVPTTASLPPSTVDGPNSCSQESNLNGNSVYLPSETSFTDSLQTPSESPATKDGHPKDSSSHGGKENREGGERQLKSPDGLESTQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIKKLQYRYEKFCPFPDTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCSGELSLARVLRKNILDKVNQKKMLKCANSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYEIRRNEDIVNFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_031954876.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031954876.1

ORF Insert Sequence:

ATGGGGAATG TTAATGAAAA ACAAAAAGCT TGGAAGCAGA GCAATTTATA CCTCTGGAAA 
CAAGATTCCC AGCGCTCTCA TCTCTCCTCC TTCACCATGA AGCTGATGGA CAAATTTCAC 
TCGCCCAAAA TCAAGAGAAC ACCATCAAAG AAAGGAAAAC CAGCCGATGT GCCTGTGAAA 
ACTTTTGAGA AACCTGTGAA CAAAAATCTC AGCTGGCTGG AAGAGAAAGA AAAAGAGGTG 
GTCAGTGCCT TGCGCTACTT TAAGACCATT GTGGACAAAA TGGCAATTGA CAAGAAGGTA 
CTGGAAATGC TTCCAGGTTC GGCCAGCAAG GTCCTGGAGG CCATCCTGCC CCTAGTGCAG 
ACAGACCCTC GCATTCAGCA GAGCTCAGCC ATCTCCTCTT GCTACAGCCG GGTGTATCAG 
AGTCTAGCCA ATCTCATTCG TTGGTCAGAT CAAGTGATGC TGGAAGGTGT GAACTCGGAA 
GATAAAGAGA TGGTGACAAC TGTGAAAGAA GTCATCAAAG CTGTTTTGGA TGGAGTGAAG 
GAGCTTGTCA GGCTCACAAT AGAGAAGCAG GAGCATCCAT CTCCCACAAG TCCAGTTAAG 
CCCAGCTCAC CAGCCTGCAA AACTGACAGC CAACCTGAGC TCCCATTGAC TGACCGGGAA 
ATGGAGATTC TCAACAAGAC AACTAGTATG TCCCAATCAA CGGAGCTGCT GCATCCTGAC 
TCCATGGATG AAGAGGTTGC TCCTCCCAAG CCCCCCCTCC CTGGCATCCG AGTGGTGGAT 
AATAGTCCTC CACCAGCTTT ACCACCCAAA AAGAGGCAGT CGGCACCATC TCCCACCAGA 
GTGGCTGTGG TTGCACCCAT GAGCCGGGCC ACCAGTGGCT CAAGTTTACC TGTTGGAATC 
AATAGACAGG ACTTTGATGT GGACTGTTAT GCCCAAAGGA GGCTGTCTGG GGGCAGCCAT 
TCATATGGCG GGGAATCCCC TCGCCTCTCC CCGTGCAGCA GCATCGGCAA GCTGAGCAAG 
TCCGACGAAC AGCTGTCCTC CTTGGACCGG GACAGTGGCC AGTGCTCCAG GAATACAAGC 
TGTGAAACAC TAGATCACTA TGACCCTGAT TATGAATTTC TACAGCAAGA CCTCTCCACT 
GCTGACCAGA TACCTCAGCA AGTAGCATGT AACCTTAGCC CATTGCCAGA ATCTGTTGGT 
GAATCTGGCT CTCCTTTCCA TGGACGTCCT TTCCAGCTGC CACCCAGCAA CTCCCCACAG 
CCAGAGCTTG GCAATCCCTT GCCCATGGGG CATCCTACGG ACACACCTCC TGCCCTGCCT 
GAGAAGAAGC GGAGGAGTGC AGCCTCTCAG ACCACGGACA GCTCCAGCTC CAGCTGTAGA 
GTCTCCTATG AGAGGCACCC TTCCCAATAT GACAATATCA TGGAGGATGA TCTCCAGAAC 
CCGGCCTCCC TCCAGCCAGG ATCATTCACG CCATTCGCTG CTGTCCTCCC CTTCCAGCAA 
GGAGGCTCTT CAGCCAATTT TGTGTCTGAT TTTGCTGCTC CCGATTCAAT GGGTGACCCC 
GAGAAACCAC CTCCTCTACC TGAAAAGAAG AACAAACACA TGCTGGCCTA CATGCAGCTC 
CTTGAGGACT ACTCAGAGCC CCAGCCTTCC ATGTTCTACC AGACGCCCCA GAACGAGCAC 
ATCTACCAGC AGAAGAACAA GCTCCTCATG GAGGTCTACG GCTTTAATGA CTCCTTCACC 
CAGGAGCTGG CCCCGCCCCC TGCCCTGCCC CCCAAGCAGA GGCAGCTGCA GGCCTCCTAT 
GCTGCCTCTT CCTTCTCTTC TGTCTCCTAC TGTGTCCAGC CAACTAAAGT GCCCTTCACG 
CCTGAGGACG CCAGTGCCAC TCAGGGTCTC GGTATGTCCA TCTCTAACTC CTTTCTCAGC 
CGGCACAGCT CCTTCCCTGT GCACTCGTAT AAATCTGTGT TTAGGTCCTA CTCCCAGGAC 
TTCATGCCTC ACAACCAAGC TTCCGTTCCA CCTTTCCTCT CGTCTACCTC CTCTTCCTCT 
CCACATTTCC CACCTGTCCA CCAGTCTCAG AGCTCTGACC TAGCAGTGCC AACCACAGCC 
AGCCTGCCTC CCAGCACTGT GGATGGGCCC AATTCCTGTT CTCAGGAGAG CAACTTGAAT 
GGGAATTCTG TCTACCTTCC TTCTGAAACC TCTTTCACTG ATTCTCTCCA GACGCCTTCG 
GAATCACCAG CTACAAAAGA TGGACATCCC AAAGATTCCT CTTCACATGG TGGGAAAGAA 
AACAGAGAAG GTGGTGAAAG GCAGCTGAAG TCCCCCGATG GTTTGGAATC GACCCAGTCA 
GAGGAGGAAG TGGATGAACT CTCTCTGATT GACCACAATG AAATTATGGC TAGGTTGACG 
TTAAAACAAG AGGGTGATGA TGGGCCAGAT GTCCGTGGGG GATCTGGGGA TATATTGCTG 
GTCCATGCAA CTGAGACCGA TAGAAAAGAC TTGGTTTTGT ACTGTGAAGC CTTTTTGACA 
ACCTACAGGA CATTTATCAC CCCTGAGGAA CTCATTAAGA AGCTACAGTA TAGATATGAG 
AAGTTCTGCC CCTTCCCAGA CACATTCAAG AAGCGAGTCA GCAAGAATAC TTTCTTCGTG 
CTGGTGCGGG TAGTGGATGA ACTGTGCCTG GTGGAATTAA CTGAAGAGAT CCTAAAGCTC 
TTGATGGAGC TGGTGTTCCG GTTAGTGTGC AGTGGGGAGC TGAGCCTTGC CCGGGTCCTC 
CGGAAGAATA TCTTGGACAA AGTGAACCAA AAGAAGATGC TAAAGTGTGC CAACTCGGAC 
CAACCCCTGG CTGCTCGAGG GGTGGCTGCC AGGCCTGGGA CTCTGCATGA CTTCCACAGC 
CATGAGATTG CTGAGCAGCT GACACTGTTG GATGCTGAGC TCTTCTACAA GATAGAGATC 
CCAGAAGTTT TGCTTTGGGC AAAAGAGCAG AATGAAGAGA AGAGCCCTAA CTTGACACAG 
TTTACTGAGC ACTTTAATAA CATGTCCTAC TGGGTTCGTT CAATAATCAT GTTGCAAGAA 
AAAGCCCAGG ACAGAGAACG GCTGCTTCTG AAATTTATCA AGATCATGAA GCACTTGAGG 
AAGCTAAATA ACTTCAACTC GTATCTGGCT ATCCTTTCAG CACTGGACTC AGCACCCATC 
CGGAGGCTGG AGTGGCAAAA ACAGACCTCA GAGGGCCTGG CCGAGTACTG CACTTTGATT 
GACAGCTCTT CCTCCTTCCG GGCCTATCGG GCGGCTCTTT CTGAGGTGGA ACCTCCATGC 
ATCCCATACC TGGGTCTGAT CCTGCAGGAC CTCACCTTTG TTCACCTCGG AAACCCAGAC 
TACATTGACG GGAAAGTGAA TTTTTCCAAG CGCTGGCAGC AGTTCAACAT CCTGGACAGT 
ATGAGATGTT TCCAGCAGGC ACATTATGAA ATCCGCAGAA ATGAGGACAT CGTGAACTTC 
TTCAATGATT TCAGTGACCA CCTAGCCGAG GAGGCCTTAT GGGAACTCTC TCTCAAAATC 
AAACCCCGGA ACATAACTCG GCGAAAAACA GATCGAGAAG AGAAGACCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_031954877.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031810737.1 Sarcophilus harrisii Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OSa226541
Clone ID Related Accession (Same CDS sequence)	XM_031954877.1
Accession Version	XM_031954877.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3432bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	rap guanine nucleotide exchange factor 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045427.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAATG TTAATGAAAA ACAAAAAGCT TGGAAGCAGA GCAATTTATA CCTCTGGAAA 
CAAGATTCCC AGCGCTCTCA TCTCTCCTCC TTCACCATGA AGCTGATGGA CAAATTTCAC 
TCGCCCAAAA TCAAGAGAAC ACCATCAAAG AAAGGAAAAC CAGCCGATGT GCCTGTGAAA 
ACTTTTGAGA AACCTGTGAA CAAAGAGGCC ACAGACAATT TTCTACCAGA GGGCTACCCT 
CCTCCCTTGG ATCTGGATCA CCAAGCAGTA CAATTTATGT CCACCAGTGC TGTGGTTCCC 
AGGTCTCAGA GGCAGAAGAA TCTCAGCTGG CTGGAAGAGA AAGAAAAAGA GGTGGTCAGT 
GCCTTGCGCT ACTTTAAGAC CATTGTGGAC AAAATGGCAA TTGACAAGAA GGTACTGGAA 
ATGCTTCCAG GTTCGGCCAG CAAGGTCCTG GAGGCCATCC TGCCCCTAGT GCAGACAGAC 
CCTCGCATTC AGCAGAGCTC AGCCATCTCC TCTTGCTACA GCCGGGTGTA TCAGAGTCTA 
GCCAATCTCA TTCGTTGGTC AGATCAAGTG ATGCTGGAAG GTGTGAACTC GGAAGATAAA 
GAGATGGTGA CAACTGTGAA AGAAGTCATC AAAGCTGTTT TGGATGGAGT GAAGGAGCTT 
GTCAGGCTCA CAATAGAGAA GCAGGAGCAT CCATCTCCCA CAAGTCCAGT TAAGCCCAGC 
TCACCAGCCT GCAAAACTGA CAGCCAACCT GAGCTCCCAT TGACTGACCG GGAAATGGAG 
ATTCTCAACA AGACAACTAG TATGTCCCAA TCAACGGAGC TGCTGCATCC TGACTCCATG 
GATGAAGAGG TTGCTCCTCC CAAGCCCCCC CTCCCTGGCA TCCGAGTGGT GGATAATAGT 
CCTCCACCAG CTTTACCACC CAAAAAGAGG CAGTCGGCAC CATCTCCCAC CAGAGTGGCT 
GTGGTTGCAC CCATGAGCCG GGCCACCAGT GGCTCAAGTT TACCTGTTGG AATCAATAGA 
CAGGACTTTG ATGTGGACTG TTATGCCCAA AGGAGGCTGT CTGGGGGCAG CCATTCATAT 
GGCGGGGAAT CCCCTCGCCT CTCCCCGTGC AGCAGCATCG GCAAGCTGAG CAAGTCCGAC 
GAACAGCTGT CCTCCTTGGA CCGGGACAGT GGCCAGTGCT CCAGGAATAC AAGCTGTGAA 
ACACTAGATC ACTATGACCC TGATTATGAA TTTCTACAGC AAGACCTCTC CACTGCTGAC 
CAGATACCTC AGCAAGTAGC ATGTAACCTT AGCCCATTGC CAGAATCTGT TGGTGAATCT 
GGCTCTCCTT TCCATGGACG TCCTTTCCAG CTGCCACCCA GCAACTCCCC ACAGCCAGAG 
CTTGGCAATC CCTTGCCCAT GGGGCATCCT ACGGACACAC CTCCTGCCCT GCCTGAGAAG 
AAGCGGAGGA GTGCAGCCTC TCAGACCACG GACAGCTCCA GCTCCAGCTG TAGAGTCTCC 
TATGAGAGGC ACCCTTCCCA ATATGACAAT ATCATGGAGG ATGATCTCCA GAACCCGGCC 
TCCCTCCAGC CAGGATCATT CACGCCATTC GCTGCTGTCC TCCCCTTCCA GCAAGGAGGC 
TCTTCAGCCA ATTTTGTGTC TGATTTTGCT GCTCCCGATT CAATGGGTGA CCCCGAGAAA 
CCACCTCCTC TACCTGAAAA GAAGAACAAA CACATGCTGG CCTACATGCA GCTCCTTGAG 
GACTACTCAG AGCCCCAGCC TTCCATGTTC TACCAGACGC CCCAGAACGA GCACATCTAC 
CAGCAGAAGA ACAAGCTCCT CATGGAGGTC TACGGCTTTA ATGACTCCTT CACCCAGGAG 
CTGGCCCCGC CCCCTGCCCT GCCCCCCAAG CAGAGGCAGC TGCAGGCCTC CTATGCTGCC 
TCTTCCTTCT CTTCTGTCTC CTACTGTGTC CAGCCAACTA AAGTGCCCTT CACGCCTGAG 
GACGCCAGTG CCACTCAGGG TCTCGGTATG TCCATCTCTA ACTCCTTTCT CAGCCGGCAC 
AGCTCCTTCC CTGTGCACTC GGAATCACCA GCTACAAAAG ATGGACATCC CAAAGATTCC 
TCTTCACATG GTGGGAAAGA AAACAGAGAA GGTGGTGAAA GGCAGCTGAA GTCCCCCGAT 
GGTTTGGAAT CGACCCAGTC AGAGGAGGAA GTGGATGAAC TCTCTCTGAT TGACCACAAT 
GAAATTATGG CTAGGTTGAC GTTAAAACAA GAGGGTGATG ATGGGCCAGA TGTCCGTGGG 
GGATCTGGGG ATATATTGCT GGTCCATGCA ACTGAGACCG ATAGAAAAGA CTTGGTTTTG 
TACTGTGAAG CCTTTTTGAC AACCTACAGG ACATTTATCA CCCCTGAGGA ACTCATTAAG 
AAGCTACAGT ATAGATATGA GAAGTTCTGC CCCTTCCCAG ACACATTCAA GAAGCGAGTC 
AGCAAGAATA CTTTCTTCGT GCTGGTGCGG GTAGTGGATG AACTGTGCCT GGTGGAATTA 
ACTGAAGAGA TCCTAAAGCT CTTGATGGAG CTGGTGTTCC GGTTAGTGTG CAGTGGGGAG 
CTGAGCCTTG CCCGGGTCCT CCGGAAGAAT ATCTTGGACA AAGTGAACCA AAAGAAGATG 
CTAAAGTGTG CCAACTCGGA CCAACCCCTG GCTGCTCGAG GGGTGGCTGC CAGGCCTGGG 
ACTCTGCATG ACTTCCACAG CCATGAGATT GCTGAGCAGC TGACACTGTT GGATGCTGAG 
CTCTTCTACA AGATAGAGAT CCCAGAAGTT TTGCTTTGGG CAAAAGAGCA GAATGAAGAG 
AAGAGCCCTA ACTTGACACA GTTTACTGAG CACTTTAATA ACATGTCCTA CTGGGTTCGT 
TCAATAATCA TGTTGCAAGA AAAAGCCCAG GACAGAGAAC GGCTGCTTCT GAAATTTATC 
AAGATCATGA AGCACTTGAG GAAGCTAAAT AACTTCAACT CGTATCTGGC TATCCTTTCA 
GCACTGGACT CAGCACCCAT CCGGAGGCTG GAGTGGCAAA AACAGACCTC AGAGGGCCTG 
GCCGAGTACT GCACTTTGAT TGACAGCTCT TCCTCCTTCC GGGCCTATCG GGCGGCTCTT 
TCTGAGGTGG AACCTCCATG CATCCCATAC CTGGGTCTGA TCCTGCAGGA CCTCACCTTT 
GTTCACCTCG GAAACCCAGA CTACATTGAC GGGAAAGTGA ATTTTTCCAA GCGCTGGCAG 
CAGTTCAACA TCCTGGACAG TATGAGATGT TTCCAGCAGG CACATTATGA AATCCGCAGA 
AATGAGGACA TCGTGAACTT CTTCAATGAT TTCAGTGACC ACCTAGCCGA GGAGGCCTTA 
TGGGAACTCT CTCTCAAAAT CAAACCCCGG AACATAACTC GGCGAAAAAC AGATCGAGAA 
GAGAAGACCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031810737.1
CDS	604..4035
Translation	MGNVNEKQKAWKQSNLYLWKQDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPADVPVKTFEKPVNKEATDNFLPEGYPPPLDLDHQAVQFMSTSAVVPRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQTDPRIQQSSAISSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKEVIKAVLDGVKELVRLTIEKQEHPSPTSPVKPSSPACKTDSQPELPLTDREMEILNKTTSMSQSTELLHPDSMDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSTADQIPQQVACNLSPLPESVGESGSPFHGRPFQLPPSNSPQPELGNPLPMGHPTDTPPALPEKKRRSAASQTTDSSSSSCRVSYERHPSQYDNIMEDDLQNPASLQPGSFTPFAAVLPFQQGGSSANFVSDFAAPDSMGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFNDSFTQELAPPPALPPKQRQLQASYAASSFSSVSYCVQPTKVPFTPEDASATQGLGMSISNSFLSRHSSFPVHSESPATKDGHPKDSSSHGGKENREGGERQLKSPDGLESTQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIKKLQYRYEKFCPFPDTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCSGELSLARVLRKNILDKVNQKKMLKCANSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYEIRRNEDIVNFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_031954877.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031954877.1

ORF Insert Sequence:

ATGGGGAATG TTAATGAAAA ACAAAAAGCT TGGAAGCAGA GCAATTTATA CCTCTGGAAA 
CAAGATTCCC AGCGCTCTCA TCTCTCCTCC TTCACCATGA AGCTGATGGA CAAATTTCAC 
TCGCCCAAAA TCAAGAGAAC ACCATCAAAG AAAGGAAAAC CAGCCGATGT GCCTGTGAAA 
ACTTTTGAGA AACCTGTGAA CAAAGAGGCC ACAGACAATT TTCTACCAGA GGGCTACCCT 
CCTCCCTTGG ATCTGGATCA CCAAGCAGTA CAATTTATGT CCACCAGTGC TGTGGTTCCC 
AGGTCTCAGA GGCAGAAGAA TCTCAGCTGG CTGGAAGAGA AAGAAAAAGA GGTGGTCAGT 
GCCTTGCGCT ACTTTAAGAC CATTGTGGAC AAAATGGCAA TTGACAAGAA GGTACTGGAA 
ATGCTTCCAG GTTCGGCCAG CAAGGTCCTG GAGGCCATCC TGCCCCTAGT GCAGACAGAC 
CCTCGCATTC AGCAGAGCTC AGCCATCTCC TCTTGCTACA GCCGGGTGTA TCAGAGTCTA 
GCCAATCTCA TTCGTTGGTC AGATCAAGTG ATGCTGGAAG GTGTGAACTC GGAAGATAAA 
GAGATGGTGA CAACTGTGAA AGAAGTCATC AAAGCTGTTT TGGATGGAGT GAAGGAGCTT 
GTCAGGCTCA CAATAGAGAA GCAGGAGCAT CCATCTCCCA CAAGTCCAGT TAAGCCCAGC 
TCACCAGCCT GCAAAACTGA CAGCCAACCT GAGCTCCCAT TGACTGACCG GGAAATGGAG 
ATTCTCAACA AGACAACTAG TATGTCCCAA TCAACGGAGC TGCTGCATCC TGACTCCATG 
GATGAAGAGG TTGCTCCTCC CAAGCCCCCC CTCCCTGGCA TCCGAGTGGT GGATAATAGT 
CCTCCACCAG CTTTACCACC CAAAAAGAGG CAGTCGGCAC CATCTCCCAC CAGAGTGGCT 
GTGGTTGCAC CCATGAGCCG GGCCACCAGT GGCTCAAGTT TACCTGTTGG AATCAATAGA 
CAGGACTTTG ATGTGGACTG TTATGCCCAA AGGAGGCTGT CTGGGGGCAG CCATTCATAT 
GGCGGGGAAT CCCCTCGCCT CTCCCCGTGC AGCAGCATCG GCAAGCTGAG CAAGTCCGAC 
GAACAGCTGT CCTCCTTGGA CCGGGACAGT GGCCAGTGCT CCAGGAATAC AAGCTGTGAA 
ACACTAGATC ACTATGACCC TGATTATGAA TTTCTACAGC AAGACCTCTC CACTGCTGAC 
CAGATACCTC AGCAAGTAGC ATGTAACCTT AGCCCATTGC CAGAATCTGT TGGTGAATCT 
GGCTCTCCTT TCCATGGACG TCCTTTCCAG CTGCCACCCA GCAACTCCCC ACAGCCAGAG 
CTTGGCAATC CCTTGCCCAT GGGGCATCCT ACGGACACAC CTCCTGCCCT GCCTGAGAAG 
AAGCGGAGGA GTGCAGCCTC TCAGACCACG GACAGCTCCA GCTCCAGCTG TAGAGTCTCC 
TATGAGAGGC ACCCTTCCCA ATATGACAAT ATCATGGAGG ATGATCTCCA GAACCCGGCC 
TCCCTCCAGC CAGGATCATT CACGCCATTC GCTGCTGTCC TCCCCTTCCA GCAAGGAGGC 
TCTTCAGCCA ATTTTGTGTC TGATTTTGCT GCTCCCGATT CAATGGGTGA CCCCGAGAAA 
CCACCTCCTC TACCTGAAAA GAAGAACAAA CACATGCTGG CCTACATGCA GCTCCTTGAG 
GACTACTCAG AGCCCCAGCC TTCCATGTTC TACCAGACGC CCCAGAACGA GCACATCTAC 
CAGCAGAAGA ACAAGCTCCT CATGGAGGTC TACGGCTTTA ATGACTCCTT CACCCAGGAG 
CTGGCCCCGC CCCCTGCCCT GCCCCCCAAG CAGAGGCAGC TGCAGGCCTC CTATGCTGCC 
TCTTCCTTCT CTTCTGTCTC CTACTGTGTC CAGCCAACTA AAGTGCCCTT CACGCCTGAG 
GACGCCAGTG CCACTCAGGG TCTCGGTATG TCCATCTCTA ACTCCTTTCT CAGCCGGCAC 
AGCTCCTTCC CTGTGCACTC GGAATCACCA GCTACAAAAG ATGGACATCC CAAAGATTCC 
TCTTCACATG GTGGGAAAGA AAACAGAGAA GGTGGTGAAA GGCAGCTGAA GTCCCCCGAT 
GGTTTGGAAT CGACCCAGTC AGAGGAGGAA GTGGATGAAC TCTCTCTGAT TGACCACAAT 
GAAATTATGG CTAGGTTGAC GTTAAAACAA GAGGGTGATG ATGGGCCAGA TGTCCGTGGG 
GGATCTGGGG ATATATTGCT GGTCCATGCA ACTGAGACCG ATAGAAAAGA CTTGGTTTTG 
TACTGTGAAG CCTTTTTGAC AACCTACAGG ACATTTATCA CCCCTGAGGA ACTCATTAAG 
AAGCTACAGT ATAGATATGA GAAGTTCTGC CCCTTCCCAG ACACATTCAA GAAGCGAGTC 
AGCAAGAATA CTTTCTTCGT GCTGGTGCGG GTAGTGGATG AACTGTGCCT GGTGGAATTA 
ACTGAAGAGA TCCTAAAGCT CTTGATGGAG CTGGTGTTCC GGTTAGTGTG CAGTGGGGAG 
CTGAGCCTTG CCCGGGTCCT CCGGAAGAAT ATCTTGGACA AAGTGAACCA AAAGAAGATG 
CTAAAGTGTG CCAACTCGGA CCAACCCCTG GCTGCTCGAG GGGTGGCTGC CAGGCCTGGG 
ACTCTGCATG ACTTCCACAG CCATGAGATT GCTGAGCAGC TGACACTGTT GGATGCTGAG 
CTCTTCTACA AGATAGAGAT CCCAGAAGTT TTGCTTTGGG CAAAAGAGCA GAATGAAGAG 
AAGAGCCCTA ACTTGACACA GTTTACTGAG CACTTTAATA ACATGTCCTA CTGGGTTCGT 
TCAATAATCA TGTTGCAAGA AAAAGCCCAG GACAGAGAAC GGCTGCTTCT GAAATTTATC 
AAGATCATGA AGCACTTGAG GAAGCTAAAT AACTTCAACT CGTATCTGGC TATCCTTTCA 
GCACTGGACT CAGCACCCAT CCGGAGGCTG GAGTGGCAAA AACAGACCTC AGAGGGCCTG 
GCCGAGTACT GCACTTTGAT TGACAGCTCT TCCTCCTTCC GGGCCTATCG GGCGGCTCTT 
TCTGAGGTGG AACCTCCATG CATCCCATAC CTGGGTCTGA TCCTGCAGGA CCTCACCTTT 
GTTCACCTCG GAAACCCAGA CTACATTGAC GGGAAAGTGA ATTTTTCCAA GCGCTGGCAG 
CAGTTCAACA TCCTGGACAG TATGAGATGT TTCCAGCAGG CACATTATGA AATCCGCAGA 
AATGAGGACA TCGTGAACTT CTTCAATGAT TTCAGTGACC ACCTAGCCGA GGAGGCCTTA 
TGGGAACTCT CTCTCAAAAT CAAACCCCGG AACATAACTC GGCGAAAAAC AGATCGAGAA 
GAGAAGACCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_012553967.3 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_012409421.3 Sarcophilus harrisii Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OSa226542
Clone ID Related Accession (Same CDS sequence)	XM_012553967.3
Accession Version	XM_012553967.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3273bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	rap guanine nucleotide exchange factor 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045427.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 31, 2019 this sequence version replaced XM_012553967.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAATG TTAATGAAAA ACAAAAAGCT TGGAAGCAGA GCAATTTATA CCTCTGGAAA 
CAAGATTCCC AGCGCTCTCA TCTCTCCTCC TTCACCATGA AGCTGATGGA CAAATTTCAC 
TCGCCCAAAA TCAAGAGAAC ACCATCAAAG AAAGGAAAAC CAGCCGATGT GCCTGTGAAA 
ACTTTTGAGA AACCTGTGAA CAAAGAGGCC ACAGACAATT TTCTACCAGA GGGCTACCCT 
CCTCCCTTGG ATCTGGATCA CCAAGCAGTA CAATTTATGT CCACCAGTGC TGTGGTTCCC 
AGGTCTCAGA GGCAGAAGAA TCTCAGCTGG CTGGAAGAGA AAGAAAAAGA GGTGGTCAGT 
GCCTTGCGCT ACTTTAAGAC CATTGTGGAC AAAATGGCAA TTGACAAGAA GGTACTGGAA 
ATGCTTCCAG GTTCGGCCAG CAAGGTCCTG GAGGCCATCC TGCCCCTAGT GCAGACAGAC 
CCTCGCATTC AGCAGAGCTC AGCCATCTCC TCTTGCTACA GCCGGGTGTA TCAGAGTCTA 
GCCAATCTCA TTCGTTGGTC AGATCAAGTG ATGCTGGAAG GTGTGAACTC GGAAGATAAA 
GAGATGGTGA CAACTGTGAA AGAAGTCATC AAAGCTGTTT TGGATGGAGT GAAGGAGCTT 
GTCAGGCTCA CAATAGAGAA GCAGGAGCAT CCATCTCCCA CAAGTCCAGT TAAGCCCAGC 
TCACCAGCCT GCAAAACTGA CAGCCAACCT GAGCTCCCAT TGACTGACCG GGAAATGGAG 
ATTCTCAACA AGACAACTAG TATGTCCCAA TCAACGGAGC TGCTGCATCC TGACTCCATG 
GATGAAGAGG TTGCTCCTCC CAAGCCCCCC CTCCCTGGCA TCCGAGTGGT GGATAATAGT 
CCTCCACCAG CTTTACCACC CAAAAAGAGG CAGTCGGCAC CATCTCCCAC CAGAGTGGCT 
GTGGTTGCAC CCATGAGCCG GGCCACCAGT GGCTCAAGTT TACCTGTTGG AATCAATAGA 
CAGGACTTTG ATGTGGACTG TTATGCCCAA AGGAGGCTGT CTGGGGGCAG CCATTCATAT 
GGCGGGGAAT CCCCTCGCCT CTCCCCGTGC AGCAGCATCG GCAAGCTGAG CAAGTCCGAC 
GAACAGCTGT CCTCCTTGGA CCGGGACAGT GGCCAGTGCT CCAGGAATAC AAGCTGTGAA 
ACACTAGATC ACTATGACCC TGATTATGAA TTTCTACAGC AAGACCTCTC CACTGCTGAC 
CAGATACCTC AGCAAGTAGC ATGTAACCTT AGCCCATTGC CAGAATCTGT TGGTGAATCT 
GGCTCTCCTT TCCATGGACG TCCTTTCCAG CTGCCACCCA GCAACTCCCC ACAGCCAGAG 
CTTGGCAATC CCTTGCCCAT GGGGCATCCT ACGGACACAC CTCCTGCCCT GCCTGAGAAG 
AAGCGGAGGA GTGCAGCCTC TCAGACCACG GACAGCTCCA GCTCCAGCTG TAGAGTCTCC 
TATGAGAGGC ACCCTTCCCA ATATGACAAT ATCATGGAGG ATGATCTCCA GAACCCGGCC 
TCCCTCCAGC CAGGATCATT CACGCCATTC GCTGCTGTCC TCCCCTTCCA GCAAGGAGGC 
TCTTCAGCCA ATTTTGTGTC TGATTTTGCT GCTCCCGATT CAATGGGTGA CCCCGAGAAA 
CCACCTCCTC TACCTGAAAA GAAGAACAAA CACATGCTGG CCTACATGCA GCTCCTTGAG 
GACTACTCAG AGCCCCAGCC TTCCATGTTC TACCAGACGC CCCAGAACGA GCACATCTAC 
CAGCAGAAGA ACAAGCTCCT CATGGAGGTC TACGGCTTTA ATGACTCCTT CACCCAGGAG 
CTGGCCCCGC CCCCTGCCCT GCCCCCCAAG CAGAGGCAGC TGGAATCACC AGCTACAAAA 
GATGGACATC CCAAAGATTC CTCTTCACAT GGTGGGAAAG AAAACAGAGA AGGTGGTGAA 
AGGCAGCTGA AGTCCCCCGA TGGTTTGGAA TCGACCCAGT CAGAGGAGGA AGTGGATGAA 
CTCTCTCTGA TTGACCACAA TGAAATTATG GCTAGGTTGA CGTTAAAACA AGAGGGTGAT 
GATGGGCCAG ATGTCCGTGG GGGATCTGGG GATATATTGC TGGTCCATGC AACTGAGACC 
GATAGAAAAG ACTTGGTTTT GTACTGTGAA GCCTTTTTGA CAACCTACAG GACATTTATC 
ACCCCTGAGG AACTCATTAA GAAGCTACAG TATAGATATG AGAAGTTCTG CCCCTTCCCA 
GACACATTCA AGAAGCGAGT CAGCAAGAAT ACTTTCTTCG TGCTGGTGCG GGTAGTGGAT 
GAACTGTGCC TGGTGGAATT AACTGAAGAG ATCCTAAAGC TCTTGATGGA GCTGGTGTTC 
CGGTTAGTGT GCAGTGGGGA GCTGAGCCTT GCCCGGGTCC TCCGGAAGAA TATCTTGGAC 
AAAGTGAACC AAAAGAAGAT GCTAAAGTGT GCCAACTCGG ACCAACCCCT GGCTGCTCGA 
GGGGTGGCTG CCAGGCCTGG GACTCTGCAT GACTTCCACA GCCATGAGAT TGCTGAGCAG 
CTGACACTGT TGGATGCTGA GCTCTTCTAC AAGATAGAGA TCCCAGAAGT TTTGCTTTGG 
GCAAAAGAGC AGAATGAAGA GAAGAGCCCT AACTTGACAC AGTTTACTGA GCACTTTAAT 
AACATGTCCT ACTGGGTTCG TTCAATAATC ATGTTGCAAG AAAAAGCCCA GGACAGAGAA 
CGGCTGCTTC TGAAATTTAT CAAGATCATG AAGCACTTGA GGAAGCTAAA TAACTTCAAC 
TCGTATCTGG CTATCCTTTC AGCACTGGAC TCAGCACCCA TCCGGAGGCT GGAGTGGCAA 
AAACAGACCT CAGAGGGCCT GGCCGAGTAC TGCACTTTGA TTGACAGCTC TTCCTCCTTC 
CGGGCCTATC GGGCGGCTCT TTCTGAGGTG GAACCTCCAT GCATCCCATA CCTGGGTCTG 
ATCCTGCAGG ACCTCACCTT TGTTCACCTC GGAAACCCAG ACTACATTGA CGGGAAAGTG 
AATTTTTCCA AGCGCTGGCA GCAGTTCAAC ATCCTGGACA GTATGAGATG TTTCCAGCAG 
GCACATTATG AAATCCGCAG AAATGAGGAC ATCGTGAACT TCTTCAATGA TTTCAGTGAC 
CACCTAGCCG AGGAGGCCTT ATGGGAACTC TCTCTCAAAA TCAAACCCCG GAACATAACT 
CGGCGAAAAA CAGATCGAGA AGAGAAGACC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012409421.3
CDS	603..3875
Translation	MGNVNEKQKAWKQSNLYLWKQDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPADVPVKTFEKPVNKEATDNFLPEGYPPPLDLDHQAVQFMSTSAVVPRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQTDPRIQQSSAISSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKEVIKAVLDGVKELVRLTIEKQEHPSPTSPVKPSSPACKTDSQPELPLTDREMEILNKTTSMSQSTELLHPDSMDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSTADQIPQQVACNLSPLPESVGESGSPFHGRPFQLPPSNSPQPELGNPLPMGHPTDTPPALPEKKRRSAASQTTDSSSSSCRVSYERHPSQYDNIMEDDLQNPASLQPGSFTPFAAVLPFQQGGSSANFVSDFAAPDSMGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFNDSFTQELAPPPALPPKQRQLESPATKDGHPKDSSSHGGKENREGGERQLKSPDGLESTQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEELIKKLQYRYEKFCPFPDTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCSGELSLARVLRKNILDKVNQKKMLKCANSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYEIRRNEDIVNFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_012553967.3
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012553967.3

ORF Insert Sequence:

ATGGGGAATG TTAATGAAAA ACAAAAAGCT TGGAAGCAGA GCAATTTATA CCTCTGGAAA 
CAAGATTCCC AGCGCTCTCA TCTCTCCTCC TTCACCATGA AGCTGATGGA CAAATTTCAC 
TCGCCCAAAA TCAAGAGAAC ACCATCAAAG AAAGGAAAAC CAGCCGATGT GCCTGTGAAA 
ACTTTTGAGA AACCTGTGAA CAAAGAGGCC ACAGACAATT TTCTACCAGA GGGCTACCCT 
CCTCCCTTGG ATCTGGATCA CCAAGCAGTA CAATTTATGT CCACCAGTGC TGTGGTTCCC 
AGGTCTCAGA GGCAGAAGAA TCTCAGCTGG CTGGAAGAGA AAGAAAAAGA GGTGGTCAGT 
GCCTTGCGCT ACTTTAAGAC CATTGTGGAC AAAATGGCAA TTGACAAGAA GGTACTGGAA 
ATGCTTCCAG GTTCGGCCAG CAAGGTCCTG GAGGCCATCC TGCCCCTAGT GCAGACAGAC 
CCTCGCATTC AGCAGAGCTC AGCCATCTCC TCTTGCTACA GCCGGGTGTA TCAGAGTCTA 
GCCAATCTCA TTCGTTGGTC AGATCAAGTG ATGCTGGAAG GTGTGAACTC GGAAGATAAA 
GAGATGGTGA CAACTGTGAA AGAAGTCATC AAAGCTGTTT TGGATGGAGT GAAGGAGCTT 
GTCAGGCTCA CAATAGAGAA GCAGGAGCAT CCATCTCCCA CAAGTCCAGT TAAGCCCAGC 
TCACCAGCCT GCAAAACTGA CAGCCAACCT GAGCTCCCAT TGACTGACCG GGAAATGGAG 
ATTCTCAACA AGACAACTAG TATGTCCCAA TCAACGGAGC TGCTGCATCC TGACTCCATG 
GATGAAGAGG TTGCTCCTCC CAAGCCCCCC CTCCCTGGCA TCCGAGTGGT GGATAATAGT 
CCTCCACCAG CTTTACCACC CAAAAAGAGG CAGTCGGCAC CATCTCCCAC CAGAGTGGCT 
GTGGTTGCAC CCATGAGCCG GGCCACCAGT GGCTCAAGTT TACCTGTTGG AATCAATAGA 
CAGGACTTTG ATGTGGACTG TTATGCCCAA AGGAGGCTGT CTGGGGGCAG CCATTCATAT 
GGCGGGGAAT CCCCTCGCCT CTCCCCGTGC AGCAGCATCG GCAAGCTGAG CAAGTCCGAC 
GAACAGCTGT CCTCCTTGGA CCGGGACAGT GGCCAGTGCT CCAGGAATAC AAGCTGTGAA 
ACACTAGATC ACTATGACCC TGATTATGAA TTTCTACAGC AAGACCTCTC CACTGCTGAC 
CAGATACCTC AGCAAGTAGC ATGTAACCTT AGCCCATTGC CAGAATCTGT TGGTGAATCT 
GGCTCTCCTT TCCATGGACG TCCTTTCCAG CTGCCACCCA GCAACTCCCC ACAGCCAGAG 
CTTGGCAATC CCTTGCCCAT GGGGCATCCT ACGGACACAC CTCCTGCCCT GCCTGAGAAG 
AAGCGGAGGA GTGCAGCCTC TCAGACCACG GACAGCTCCA GCTCCAGCTG TAGAGTCTCC 
TATGAGAGGC ACCCTTCCCA ATATGACAAT ATCATGGAGG ATGATCTCCA GAACCCGGCC 
TCCCTCCAGC CAGGATCATT CACGCCATTC GCTGCTGTCC TCCCCTTCCA GCAAGGAGGC 
TCTTCAGCCA ATTTTGTGTC TGATTTTGCT GCTCCCGATT CAATGGGTGA CCCCGAGAAA 
CCACCTCCTC TACCTGAAAA GAAGAACAAA CACATGCTGG CCTACATGCA GCTCCTTGAG 
GACTACTCAG AGCCCCAGCC TTCCATGTTC TACCAGACGC CCCAGAACGA GCACATCTAC 
CAGCAGAAGA ACAAGCTCCT CATGGAGGTC TACGGCTTTA ATGACTCCTT CACCCAGGAG 
CTGGCCCCGC CCCCTGCCCT GCCCCCCAAG CAGAGGCAGC TGGAATCACC AGCTACAAAA 
GATGGACATC CCAAAGATTC CTCTTCACAT GGTGGGAAAG AAAACAGAGA AGGTGGTGAA 
AGGCAGCTGA AGTCCCCCGA TGGTTTGGAA TCGACCCAGT CAGAGGAGGA AGTGGATGAA 
CTCTCTCTGA TTGACCACAA TGAAATTATG GCTAGGTTGA CGTTAAAACA AGAGGGTGAT 
GATGGGCCAG ATGTCCGTGG GGGATCTGGG GATATATTGC TGGTCCATGC AACTGAGACC 
GATAGAAAAG ACTTGGTTTT GTACTGTGAA GCCTTTTTGA CAACCTACAG GACATTTATC 
ACCCCTGAGG AACTCATTAA GAAGCTACAG TATAGATATG AGAAGTTCTG CCCCTTCCCA 
GACACATTCA AGAAGCGAGT CAGCAAGAAT ACTTTCTTCG TGCTGGTGCG GGTAGTGGAT 
GAACTGTGCC TGGTGGAATT AACTGAAGAG ATCCTAAAGC TCTTGATGGA GCTGGTGTTC 
CGGTTAGTGT GCAGTGGGGA GCTGAGCCTT GCCCGGGTCC TCCGGAAGAA TATCTTGGAC 
AAAGTGAACC AAAAGAAGAT GCTAAAGTGT GCCAACTCGG ACCAACCCCT GGCTGCTCGA 
GGGGTGGCTG CCAGGCCTGG GACTCTGCAT GACTTCCACA GCCATGAGAT TGCTGAGCAG 
CTGACACTGT TGGATGCTGA GCTCTTCTAC AAGATAGAGA TCCCAGAAGT TTTGCTTTGG 
GCAAAAGAGC AGAATGAAGA GAAGAGCCCT AACTTGACAC AGTTTACTGA GCACTTTAAT 
AACATGTCCT ACTGGGTTCG TTCAATAATC ATGTTGCAAG AAAAAGCCCA GGACAGAGAA 
CGGCTGCTTC TGAAATTTAT CAAGATCATG AAGCACTTGA GGAAGCTAAA TAACTTCAAC 
TCGTATCTGG CTATCCTTTC AGCACTGGAC TCAGCACCCA TCCGGAGGCT GGAGTGGCAA 
AAACAGACCT CAGAGGGCCT GGCCGAGTAC TGCACTTTGA TTGACAGCTC TTCCTCCTTC 
CGGGCCTATC GGGCGGCTCT TTCTGAGGTG GAACCTCCAT GCATCCCATA CCTGGGTCTG 
ATCCTGCAGG ACCTCACCTT TGTTCACCTC GGAAACCCAG ACTACATTGA CGGGAAAGTG 
AATTTTTCCA AGCGCTGGCA GCAGTTCAAC ATCCTGGACA GTATGAGATG TTTCCAGCAG 
GCACATTATG AAATCCGCAG AAATGAGGAC ATCGTGAACT TCTTCAATGA TTTCAGTGAC 
CACCTAGCCG AGGAGGCCTT ATGGGAACTC TCTCTCAAAA TCAAACCCCG GAACATAACT 
CGGCGAAAAA CAGATCGAGA AGAGAAGACC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	RAPGEF1
Entrez Gene ID	100914252
Full Name	Rap guanine nucleotide exchange factor 1
Gene Type	protein-coding
Organism	Sarcophilus harrisii(Tasmanian devil)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

RAPGEF1 cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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