LOC8285021 cDNA ORF clone, Ricinus communis(castor bean)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC8285021
Entrez Gene ID	8285021
Full Name	phosphoenolpyruvate carboxylase 2
Synonyms	PPC1
Gene Type	protein-coding
Organism	Ricinus communis(castor bean)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001323767.1	NP_001310696.1	phosphoenolpyruvate carboxylase 2

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Phosphorylation of bacterial-type phosphoenolpyruvate carboxylase at Ser425 provides a further tier of enzyme control in developing castor oil seeds.
O'Leary B, Rao SK, Plaxton WC
The Biochemical journal433(1)65-74(2011 Jan)

Bacterial- and plant-type phosphoenolpyruvate carboxylase polypeptides interact in the hetero-oligomeric Class-2 PEPC complex of developing castor oil seeds.
Gennidakis S, Rao S, Greenham K, Uhrig RG, O'Leary B, Snedden WA, Lu C, Plaxton WC
The Plant journal : for cell and molecular biology52(5)839-49(2007 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC8285021 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC8285021 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORb40850	NM_001323767.1 Latest version!	Ricinus communis phosphoenolpyruvate carboxylase 2 (LOC8285021), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC8285021 ( NM_001323767.1 ) cDNA ORF clone, Ricinus communis(castor bean) -> NP_001310696.1 Ricinus communis phosphoenolpyruvate carboxylase 2 (LOC8285021), mRNA.

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CloneID	ORb40850
Clone ID Related Accession (Same CDS sequence)	NM_001323767.1
Accession Version	NM_001323767.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2898bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556899200000
Organism	Ricinus communis(castor bean)
Product	phosphoenolpyruvate carboxylase 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from EF634319.1. On Apr 22, 2016 this sequence version replaced XM_002517991.2. ##Evidence-Data-START## Transcript exon combination :: EF634319.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1034164, SAMEA1034165 [ECO:0000348] ##Evidence-Data-END##

ATGGCTGGTA GAAATTTAGT CATTATGGCA TCAATAGATG CACAGTTGAG GTTATTGGCC 
CTAAGGAAAG TATCTGAGGA TGATAAGTTG GTCGAGTATG ACGCTCTGTT ATTGGACCGA 
TTTCTCGATA TCTTGCAGGA TTTGCATGGA GAGGATATTA GAGAAACTGT TCAAGACTGT 
TATGAACTTT CAGCTGAGTA TGAAGGGAAA CACAACCCTC AAAAGTTGGC GGAACTTGGG 
AAAGTGCTAA CAAGTTTGGA TCCTGGGGAT TCAATTGTTG TTACGAAGTC ATTCTCTCAC 
ATGCTTAACT TGGCCAATCT GGCTGAGGAA GTTCAGATCG CTTATAGACG AAGAATCAAA 
TTGAAGAAAG GAGATTTTGC TGATGAGAAC TCAGCAACAA CTGAATCAGA CATTGAAGAG 
ACCCTAAAAA GACTGGTGGT GCAATTGAAG AAATCCCCAG AAGAAGTTTT TGATGCTTTG 
AAGAACCAGA CTGTGGATTT AGTCTTAACT GCACATCCTA CTCAATCTGT TAGAAGATCT 
TTGCTTCAAA AGCACGCAAG GATACGGGAC TGTTTGACTC AGTTGTACGC TAAGGACATT 
ACTCCAGATG ATAAGCAGGA ACTTGATGAG GCCTTACAAA GAGAGATTCA AGCTGCATTC 
CGCACAGATG AAATCCGAAG GACTCCTCCG ACTCCACAAG ATGAGATGAG AGCAGGAATG 
AGCTACTTCC ATGAGACTAT ATGGAAGGGT GTCCCGAAGT TCTTGCGCCG TGTTGACACA 
GCTTTGAAGA ATATTGGGAT AAATGAGCGA GTTCCTTATA ATGCTCCTCT CATCCAATTC 
TCCTCTTGGA TGGGTGGAGA TCGTGATGGA AATCCTCGAG TAACTCCTGA AGTTACAAGG 
GATGTTTGCT TACTAGCTAG AATGATGGCT GCTAACTTGT ATTTCTCTCA AATAGAGGAT 
CTTATGTTTG AGTTGTCTAT GTGGCGGTGT AACGAAGAAC TCCGTGTTCG TGCAGATGAA 
CTTCATAGGA CGTCAAGGAA AGATGCCAAA CACTACATAG AATTTTGGAA ACAGATCCCC 
CCAAGTGAGC CTTATCGAGT TATTCTTGGT GATGTAAGAG ACAAACTTTA TAATACGCGC 
GAACGCTCTC GTCAATTATT AGCTAATGGG ATTTCTGACA TTCCTGAGGA AGCGACTTTT 
ACTAATGTGG AGCAGTTTCT GGAGCCTCTT GAACTCTGTT ACAGATCCCT TTGTGCTTGT 
GGTGATCGGC CAATTGCAGA TGGAAGCCTG CTTGATTTCC TACGGCAAGT TTCTACTTTT 
GGACTTTCAC TTGTGAGACT TGATATTCGA CAGGAATCTG AAAGGCATAC AGATGTACTT 
GATGCTATCA CAAAGCATTT GGGGATTGGC TTTTATCGTG AATGGTCTGA AGAGCATAGG 
CAAGAATGGC TTTTAACAGA GCTCAGAGGC AAACGTCCTC TTTTTGGCCC CGATCTTCCC 
AAAACAGATG AAATTGCTGA TGTACTAGAC ACATTCCATG TTATAGCAGA ACTTCCGGCA 
GACAACTTTG GTGCCTATAT AATCTCAATG GCTACAGCGC CATCTGATGT GCTAGCTGTT 
GAGCTCTTAC AACGTGAATG CCGTGTTAAA CAACCATTGA GGGTTGTTCC ATTGTTTGAA 
AAGCTTGCTG ATCTTGAAGC TGCTCCTGCT GCTGTGGCCC GTCTTTTCTC AATAGATTGG 
TATAGAAACC GGATCAATGG AAAGCAAGAA GTCATGATAG GGTACTCAGA TTCAGGAAAG 
GATGCTGGGC GTCTCTCTGC AGCTTGGCAG TTATATAAGG CTCAAGAAGA GCTTGTGAAG 
GTAGCAAAGC AGTATGGAGT GAAGCTAACA ATGTTCCATG GTCGAGGAGG GACAGTTGGA 
AGAGGAGGTG GGCCCACTCA TCTTGCTATC TTATCTCAAC CACCAGATAC CATTCATGGT 
TCACTCCGAG TGACGGTTCA AGGTGAAGTT ATTGAACAGT CATTTGGGGA GGAGCACTTA 
TGCTTCAGAA CCCTCCAACG TTTTACAGCA GCCACACTTG AGCATGGAAT GCATCCTCCA 
GTCTCACCAA AGCCAGAATG GCGGACACTC CTGGATGAGA TGGCAGTTAT TGCCACTAAA 
GAGTATCGTT CCATAGTCTT TCAAGAACCT CGATTTGTAG AGTACTTTCG CCTTGCAACA 
CCAGAGCTGG AGTATGGTCG CATGAATATT GGAAGTCGAC CATCTAAAAG GAAGCCAAGT 
GGCGGAATCG AATCACTTCG AGCAATCCCA TGGATCTTTG CATGGACTCA AACAAGGTTT 
CATCTACCAG TTTGGCTTGG CTTTGGGCCA GCATTTAAGC ATGTCATTGA AAAAGATGTA 
AGGAATCTTC ACATGCTCCA GGAAATGTAC AATCAATGGC CCTTCTTTAG GGTCACAATT 
GACTTGGTCG AAATGGTGTT CGCAAAGGGT GATCCAGGAA TAGCTGCTTT ATATGATAAA 
CTCCTAGTGT CAGAAGAATT GTGGCCATTT GGAGAGCGCC TGAGAGTGAA CTATGAAGAA 
ACTAAGCACC TTCTCCTACA GGTTGCGGGG CATAAAGATC TTCTTGAAGG AGACCCTTAC 
TTGAAGCAAA GGCTTCGACT TCGTGATGCC TATATCACAA CCCTTAATGT TTGCCAAGCT 
TACACTCTGA AGCGGATTCG TGATCCTGAC TACCATGTGA CGCTCCGGCC ACACTTGTCA 
AAAGAACATA TGGAATCGAG CAAACCAGCT GCAGAGCTTG TCAAACTTAA TCCAAGAAGT 
GAGTATGCTC CTGGTCTGGA AGACACTCTC ATTCTGACCA TGAAGGGCAT TGCTGCTGGT 
ATGCAGAACA CTGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001310696.1
CDS	12..2909
Translation	MAGRNLVIMASIDAQLRLLALRKVSEDDKLVEYDALLLDRFLDILQDLHGEDIRETVQDCYELSAEYEGKHNPQKLAELGKVLTSLDPGDSIVVTKSFSHMLNLANLAEEVQIAYRRRIKLKKGDFADENSATTESDIEETLKRLVVQLKKSPEEVFDALKNQTVDLVLTAHPTQSVRRSLLQKHARIRDCLTQLYAKDITPDDKQELDEALQREIQAAFRTDEIRRTPPTPQDEMRAGMSYFHETIWKGVPKFLRRVDTALKNIGINERVPYNAPLIQFSSWMGGDRDGNPRVTPEVTRDVCLLARMMAANLYFSQIEDLMFELSMWRCNEELRVRADELHRTSRKDAKHYIEFWKQIPPSEPYRVILGDVRDKLYNTRERSRQLLANGISDIPEEATFTNVEQFLEPLELCYRSLCACGDRPIADGSLLDFLRQVSTFGLSLVRLDIRQESERHTDVLDAITKHLGIGFYREWSEEHRQEWLLTELRGKRPLFGPDLPKTDEIADVLDTFHVIAELPADNFGAYIISMATAPSDVLAVELLQRECRVKQPLRVVPLFEKLADLEAAPAAVARLFSIDWYRNRINGKQEVMIGYSDSGKDAGRLSAAWQLYKAQEELVKVAKQYGVKLTMFHGRGGTVGRGGGPTHLAILSQPPDTIHGSLRVTVQGEVIEQSFGEEHLCFRTLQRFTAATLEHGMHPPVSPKPEWRTLLDEMAVIATKEYRSIVFQEPRFVEYFRLATPELEYGRMNIGSRPSKRKPSGGIESLRAIPWIFAWTQTRFHLPVWLGFGPAFKHVIEKDVRNLHMLQEMYNQWPFFRVTIDLVEMVFAKGDPGIAALYDKLLVSEELWPFGERLRVNYEETKHLLLQVAGHKDLLEGDPYLKQRLRLRDAYITTLNVCQAYTLKRIRDPDYHVTLRPHLSKEHMESSKPAAELVKLNPRSEYAPGLEDTLILTMKGIAAGMQNTG

Target ORF information:

RefSeq Version	NM_001323767.1
Organism	Ricinus communis(castor bean)
Definition	Ricinus communis phosphoenolpyruvate carboxylase 2 (LOC8285021), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001323767.1

ORF Insert Sequence:

ATGGCTGGTA GAAATTTAGT CATTATGGCA TCAATAGATG CACAGTTGAG GTTATTGGCC 
CTAAGGAAAG TATCTGAGGA TGATAAGTTG GTCGAGTATG ACGCTCTGTT ATTGGACCGA 
TTTCTCGATA TCTTGCAGGA TTTGCATGGA GAGGATATTA GAGAAACTGT TCAAGACTGT 
TATGAACTTT CAGCTGAGTA TGAAGGGAAA CACAACCCTC AAAAGTTGGC GGAACTTGGG 
AAAGTGCTAA CAAGTTTGGA TCCTGGGGAT TCAATTGTTG TTACGAAGTC ATTCTCTCAC 
ATGCTTAACT TGGCCAATCT GGCTGAGGAA GTTCAGATCG CTTATAGACG AAGAATCAAA 
TTGAAGAAAG GAGATTTTGC TGATGAGAAC TCAGCAACAA CTGAATCAGA CATTGAAGAG 
ACCCTAAAAA GACTGGTGGT GCAATTGAAG AAATCCCCAG AAGAAGTTTT TGATGCTTTG 
AAGAACCAGA CTGTGGATTT AGTCTTAACT GCACATCCTA CTCAATCTGT TAGAAGATCT 
TTGCTTCAAA AGCACGCAAG GATACGGGAC TGTTTGACTC AGTTGTACGC TAAGGACATT 
ACTCCAGATG ATAAGCAGGA ACTTGATGAG GCCTTACAAA GAGAGATTCA AGCTGCATTC 
CGCACAGATG AAATCCGAAG GACTCCTCCG ACTCCACAAG ATGAGATGAG AGCAGGAATG 
AGCTACTTCC ATGAGACTAT ATGGAAGGGT GTCCCGAAGT TCTTGCGCCG TGTTGACACA 
GCTTTGAAGA ATATTGGGAT AAATGAGCGA GTTCCTTATA ATGCTCCTCT CATCCAATTC 
TCCTCTTGGA TGGGTGGAGA TCGTGATGGA AATCCTCGAG TAACTCCTGA AGTTACAAGG 
GATGTTTGCT TACTAGCTAG AATGATGGCT GCTAACTTGT ATTTCTCTCA AATAGAGGAT 
CTTATGTTTG AGTTGTCTAT GTGGCGGTGT AACGAAGAAC TCCGTGTTCG TGCAGATGAA 
CTTCATAGGA CGTCAAGGAA AGATGCCAAA CACTACATAG AATTTTGGAA ACAGATCCCC 
CCAAGTGAGC CTTATCGAGT TATTCTTGGT GATGTAAGAG ACAAACTTTA TAATACGCGC 
GAACGCTCTC GTCAATTATT AGCTAATGGG ATTTCTGACA TTCCTGAGGA AGCGACTTTT 
ACTAATGTGG AGCAGTTTCT GGAGCCTCTT GAACTCTGTT ACAGATCCCT TTGTGCTTGT 
GGTGATCGGC CAATTGCAGA TGGAAGCCTG CTTGATTTCC TACGGCAAGT TTCTACTTTT 
GGACTTTCAC TTGTGAGACT TGATATTCGA CAGGAATCTG AAAGGCATAC AGATGTACTT 
GATGCTATCA CAAAGCATTT GGGGATTGGC TTTTATCGTG AATGGTCTGA AGAGCATAGG 
CAAGAATGGC TTTTAACAGA GCTCAGAGGC AAACGTCCTC TTTTTGGCCC CGATCTTCCC 
AAAACAGATG AAATTGCTGA TGTACTAGAC ACATTCCATG TTATAGCAGA ACTTCCGGCA 
GACAACTTTG GTGCCTATAT AATCTCAATG GCTACAGCGC CATCTGATGT GCTAGCTGTT 
GAGCTCTTAC AACGTGAATG CCGTGTTAAA CAACCATTGA GGGTTGTTCC ATTGTTTGAA 
AAGCTTGCTG ATCTTGAAGC TGCTCCTGCT GCTGTGGCCC GTCTTTTCTC AATAGATTGG 
TATAGAAACC GGATCAATGG AAAGCAAGAA GTCATGATAG GGTACTCAGA TTCAGGAAAG 
GATGCTGGGC GTCTCTCTGC AGCTTGGCAG TTATATAAGG CTCAAGAAGA GCTTGTGAAG 
GTAGCAAAGC AGTATGGAGT GAAGCTAACA ATGTTCCATG GTCGAGGAGG GACAGTTGGA 
AGAGGAGGTG GGCCCACTCA TCTTGCTATC TTATCTCAAC CACCAGATAC CATTCATGGT 
TCACTCCGAG TGACGGTTCA AGGTGAAGTT ATTGAACAGT CATTTGGGGA GGAGCACTTA 
TGCTTCAGAA CCCTCCAACG TTTTACAGCA GCCACACTTG AGCATGGAAT GCATCCTCCA 
GTCTCACCAA AGCCAGAATG GCGGACACTC CTGGATGAGA TGGCAGTTAT TGCCACTAAA 
GAGTATCGTT CCATAGTCTT TCAAGAACCT CGATTTGTAG AGTACTTTCG CCTTGCAACA 
CCAGAGCTGG AGTATGGTCG CATGAATATT GGAAGTCGAC CATCTAAAAG GAAGCCAAGT 
GGCGGAATCG AATCACTTCG AGCAATCCCA TGGATCTTTG CATGGACTCA AACAAGGTTT 
CATCTACCAG TTTGGCTTGG CTTTGGGCCA GCATTTAAGC ATGTCATTGA AAAAGATGTA 
AGGAATCTTC ACATGCTCCA GGAAATGTAC AATCAATGGC CCTTCTTTAG GGTCACAATT 
GACTTGGTCG AAATGGTGTT CGCAAAGGGT GATCCAGGAA TAGCTGCTTT ATATGATAAA 
CTCCTAGTGT CAGAAGAATT GTGGCCATTT GGAGAGCGCC TGAGAGTGAA CTATGAAGAA 
ACTAAGCACC TTCTCCTACA GGTTGCGGGG CATAAAGATC TTCTTGAAGG AGACCCTTAC 
TTGAAGCAAA GGCTTCGACT TCGTGATGCC TATATCACAA CCCTTAATGT TTGCCAAGCT 
TACACTCTGA AGCGGATTCG TGATCCTGAC TACCATGTGA CGCTCCGGCC ACACTTGTCA 
AAAGAACATA TGGAATCGAG CAAACCAGCT GCAGAGCTTG TCAAACTTAA TCCAAGAAGT 
GAGTATGCTC CTGGTCTGGA AGACACTCTC ATTCTGACCA TGAAGGGCAT TGCTGCTGGT 
ATGCAGAACA CTGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Phosphorylation of bacterial-type phosphoenolpyruvate carboxylase at Ser425 provides a further tier of enzyme control in developing castor oil seeds.
The Biochemical journal433(1)65-74(2011 Jan)
O'Leary B,Rao SK,Plaxton WC

Bacterial- and plant-type phosphoenolpyruvate carboxylase polypeptides interact in the hetero-oligomeric Class-2 PEPC complex of developing castor oil seeds.
The Plant journal : for cell and molecular biology52(5)839-49(2007 Dec)
Gennidakis S,Rao S,Greenham K,Uhrig RG,O'Leary B,Snedden WA,Lu C,Plaxton WC

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LOC8285021 cDNA ORF clone, Ricinus communis(castor bean)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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