LOC8276899 cDNA ORF clone, Ricinus communis(castor bean)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC8276899
Entrez Gene ID	8276899
Full Name	methionine S-methyltransferase
Gene Type	protein-coding
Organism	Ricinus communis(castor bean)

mRNA and Protein(s)

mRNA	Protein	Name
XM_025158568.1	XP_025014336.1	methionine S-methyltransferase
XM_002525821.2	XP_002525867.1	methionine S-methyltransferase

Pathways from BioSystems
Gene Ontology

The following LOC8276899 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC8276899 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORb16703	XM_002525821.2 Latest version!	Ricinus communis methionine S-methyltransferase (LOC8276899), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORb53478	XM_025158568.1 Latest version!	Ricinus communis methionine S-methyltransferase (LOC8276899), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC8276899 ( XM_002525821.2 ) cDNA ORF clone, Ricinus communis(castor bean) -> XP_002525867.1 Ricinus communis methionine S-methyltransferase (LOC8276899), mRNA.

Price & Availability↑

CloneID	ORb16703
Clone ID Related Accession (Same CDS sequence)	XM_002525821.2
Accession Version	XM_002525821.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3006bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1456243200000
Organism	Ricinus communis(castor bean)
Product	methionine S-methyltransferase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_002994479.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 25, 2016 this sequence version replaced XM_002525821.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ricinus communis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGTGA TTCCTAGCAT TTTTATCCCT GAAGACTGGT CGTTCACTTT TTATGAGGGA 
CTGAATAGGC ATCCCGATTC AATTTTCAAG GACAAGACGG TTGCTGAACT TGGTTGTGGA 
AATGGATGGA TATCAATTGC TATTGCTGAT AAGTGGTTGC CTTCTAAGGT GTATGGTCTT 
GATATTAATC CTAGAGCAGT CAAGGTTTCT TGGATAAACT TATACTTAAA TGCTTTGGAT 
GAAAATGGTC AACCCATCTA TGATGCGGAA AAGAAAACTT TACTGGACAG GGTGCAGTTT 
CATGAGTCTG ATCTACTTTC TTATTGTAGA GATCATGACA TTCAACTTGA ACGAATTGTT 
GGATGCATAC CTCAGATTCT TAACCCAAAT CCAGATGCAA TGTCTAAAAT GATTACAGAA 
AATGCAAGTG AAGAGTTTCT GTATTCATTG AGTAACTACT GTGCACTTCA GGGGTTTGTT 
GAGGATCAAT TTGGCCTTGG ACTAATTGCC AGGGCTGTTG AGGAAGGAAT ATCTGTCATC 
AAACCTATGG GGATTATGAT CTTTAATATG GGCGGCCGTC CAGGACAAGC TGTTTGTAAA 
CGCTTGTTTG AAAGGCGTGG ATTTCATGTA AACAAGCTTT GGCAGACTAA AGTTATTCAG 
GCTGCTGACA CAGACATTTC AGCCTTAGTT GAAATTGAGA AAAACAGTCC ACACCGGTTT 
GAGTTTTTTA TGGGTCTTTC TGGAGATCAG CCTATTTGTG CTCGAACAGC ATGGGCCTAT 
GGGAAGGCTG GTGGCCGAAT TGCTCATGCT TTGTCAGTGT ACAGTTGTCA ACTTCGTCAA 
CCAAACCAGG TTAAGAAAAT TTTCGAGTTC CTTAAAAATG GCTTCCATGA AGTAAGCAGT 
TCATTGGATT TATCTTTTGA GGATGATTCC GTTGCTGATG AGAAGATTCC ATTCCTAGCT 
TTTCTTGCTA GTGAATTGAA AGAAAAATCA TGTTTCCCCT ATGAATCACC AGCAGGAAGC 
ATATATTTCC GTAATCTAAT TGCAGGCTTT TTGAAGATAT ACCACCATAT TCCACTGAAA 
TCCAATAATG TCGTCATCTT TCCTTCAAGG GCTGTCGCAA TTGAACACAC TCTTCGATTG 
TTCTCACCTC GCGTTGCAAT TGTTGATGAA CATCTGACTC GACACCTACC CCGACAGTGG 
TTGACATCTT TAGCAATTGA GGGTACGGAA AATTATGACC CTTCAAAGGA TGTAATTACA 
GTCATTGATG CACCAAGGCA ATCTGATTTA ATGGTAGAGC TGATAAAGAA GCTCAAGCCA 
CAGGTGGTGA TTACTGGGAT GGCTCAATTT GAGGCTGTAA CCAGTTCAGC TTTTGTTCAG 
CTCTTGGATG TTACTAGGGA AATTGGATCT CGTCTTTTCT TAGACATATC TGATCACCTG 
GAACTCTCCA GCCTTCCCAG TCCCAATGGA GTTCTAAAAT ATCTTGCAGG AACTCGTCTA 
CCTTCACATG CTGCAATCCT ATGTGGTTTT GTAAAGAACA AGGTTTATTC AGATTTGGAA 
GTGGCTTTTG TCATTTCAGA AGAGGAGGCA GTTTTTAAGG CCTTGTCCAA GACTGTAGAA 
GTGCTAGAAG GAAATACTGC ACCAATCAGG CAGTTGTATT ATGGTTGCTT GTTTCATGAG 
CTTCTAGCTT TTCAGCTTGT TGACCGACAT CCGCTTCCAG AGAGGGATTT TGAAAAAGTC 
AAATCAGTTG AGGCGATTGG CTTTGCTAGT TCTGCAATTC CAGTTCTCAA TGATTCAGAG 
TTGTCAATAA GTGAGGAAGA AAAGTCTTCC TTAATCCACA TGGATATAGA TCAAAGTTTT 
ATGCCCATTC CATCTCCTGT GAAGGCTGCA ATATTTGAAA GTTTTGCAAG ACAGAACATG 
GCTGAGTCAG AAATTGATGT CACCCCAAGC ATCAAACAGT TCATCAAGAA CAATTATGGG 
TTTCCTATGG ACAATAAAAC AGAGTTTGTA TATGCTGACT TCTCACAAGC CCTGTTTAAT 
AGACTGATTC TTTGCTGCAT CCAAGAAGGC GGAACATTTT GTTTCCCTGC TGGCTCAAAC 
GGCAATTATG TCTCTGCCGC CAAGTTCTTA AAAGCAAATG TCATGAGTAT CCCTACTGAC 
TCTGGATCTG GCTTCAAACT GACAGACAAG CTACTTGATG GAGTACTTGA TACCGTGAAT 
AAACCATGGG TGTATATTTC TGGTCCAACA ATTACCCCTA CAGGCTTGCT TTACAGCAAT 
AAAGAGATGG AGAACATACT AACTACCTGT GCAAGATTTG GGGCCAGAGT AATCATTGAT 
ACTTCCTTTT CAGGATTGGA GTTTGAAGGT TGGGGTGGTT GGAATTTGGA GACAACATCA 
TCGAAGTTGA ATTCCTCTAA CAATCCATCT TTTTGTGTCT CTCTGATTGG AGGACTGTCT 
CTGAAGCTCT CAAGCGGAGT ACTCAAATTT GGTTATTTGG TTTTAAACGA TCCATTTTTG 
GTTGATGCAT TTTATAGCTT TCCAGGACTA AGCAAACCCC ATAGCACTGT TAAGTATGCT 
ATAAAGAAGC TGTTGAGTCT GAATGAGCAG AAAGCAAGAG ACCTGACAGA TGCTGTTGCA 
GAACAAACAA GAAATTTGAA ATCCAGATCC CAGCGCATGA AAGAGACACT AGAGAAATGC 
GGGTGGGATG TGTTGGAACC TCGTGGCGGG GTGTCCATGA TTGCAAAGCC TTCTGCCTAT 
CTAAACAAGG TTGTTAAGAT CAAGCATTCA CCTGAAAATG ATGAGGAAAA TTCCACTGCT 
TATGAAGTCA AGCTGGATGA CTCAAATATC AGGGAAGCCA TTGTCAGGTC CACAGGTTTG 
TGTATCAACA GTGGTGTATG GACTGGAATT CCTGGTTACT GCCGCTTCAC AATTGCTCTG 
GAAGAACGAG ACTTCGAGCG AGCATTAAAT TGCATAATCA AGTTTAAGGA TCTCATTGGC 
AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002525867.1
CDS	339..3344
Translation	MMVIPSIFIPEDWSFTFYEGLNRHPDSIFKDKTVAELGCGNGWISIAIADKWLPSKVYGLDINPRAVKVSWINLYLNALDENGQPIYDAEKKTLLDRVQFHESDLLSYCRDHDIQLERIVGCIPQILNPNPDAMSKMITENASEEFLYSLSNYCALQGFVEDQFGLGLIARAVEEGISVIKPMGIMIFNMGGRPGQAVCKRLFERRGFHVNKLWQTKVIQAADTDISALVEIEKNSPHRFEFFMGLSGDQPICARTAWAYGKAGGRIAHALSVYSCQLRQPNQVKKIFEFLKNGFHEVSSSLDLSFEDDSVADEKIPFLAFLASELKEKSCFPYESPAGSIYFRNLIAGFLKIYHHIPLKSNNVVIFPSRAVAIEHTLRLFSPRVAIVDEHLTRHLPRQWLTSLAIEGTENYDPSKDVITVIDAPRQSDLMVELIKKLKPQVVITGMAQFEAVTSSAFVQLLDVTREIGSRLFLDISDHLELSSLPSPNGVLKYLAGTRLPSHAAILCGFVKNKVYSDLEVAFVISEEEAVFKALSKTVEVLEGNTAPIRQLYYGCLFHELLAFQLVDRHPLPERDFEKVKSVEAIGFASSAIPVLNDSELSISEEEKSSLIHMDIDQSFMPIPSPVKAAIFESFARQNMAESEIDVTPSIKQFIKNNYGFPMDNKTEFVYADFSQALFNRLILCCIQEGGTFCFPAGSNGNYVSAAKFLKANVMSIPTDSGSGFKLTDKLLDGVLDTVNKPWVYISGPTITPTGLLYSNKEMENILTTCARFGARVIIDTSFSGLEFEGWGGWNLETTSSKLNSSNNPSFCVSLIGGLSLKLSSGVLKFGYLVLNDPFLVDAFYSFPGLSKPHSTVKYAIKKLLSLNEQKARDLTDAVAEQTRNLKSRSQRMKETLEKCGWDVLEPRGGVSMIAKPSAYLNKVVKIKHSPENDEENSTAYEVKLDDSNIREAIVRSTGLCINSGVWTGIPGYCRFTIALEERDFERALNCIIKFKDLIGN

Target ORF information:

RefSeq Version	XM_002525821.2
Organism	Ricinus communis(castor bean)
Definition	Ricinus communis methionine S-methyltransferase (LOC8276899), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002525821.2

ORF Insert Sequence:

ATGATGGTGA TTCCTAGCAT TTTTATCCCT GAAGACTGGT CGTTCACTTT TTATGAGGGA 
CTGAATAGGC ATCCCGATTC AATTTTCAAG GACAAGACGG TTGCTGAACT TGGTTGTGGA 
AATGGATGGA TATCAATTGC TATTGCTGAT AAGTGGTTGC CTTCTAAGGT GTATGGTCTT 
GATATTAATC CTAGAGCAGT CAAGGTTTCT TGGATAAACT TATACTTAAA TGCTTTGGAT 
GAAAATGGTC AACCCATCTA TGATGCGGAA AAGAAAACTT TACTGGACAG GGTGCAGTTT 
CATGAGTCTG ATCTACTTTC TTATTGTAGA GATCATGACA TTCAACTTGA ACGAATTGTT 
GGATGCATAC CTCAGATTCT TAACCCAAAT CCAGATGCAA TGTCTAAAAT GATTACAGAA 
AATGCAAGTG AAGAGTTTCT GTATTCATTG AGTAACTACT GTGCACTTCA GGGGTTTGTT 
GAGGATCAAT TTGGCCTTGG ACTAATTGCC AGGGCTGTTG AGGAAGGAAT ATCTGTCATC 
AAACCTATGG GGATTATGAT CTTTAATATG GGCGGCCGTC CAGGACAAGC TGTTTGTAAA 
CGCTTGTTTG AAAGGCGTGG ATTTCATGTA AACAAGCTTT GGCAGACTAA AGTTATTCAG 
GCTGCTGACA CAGACATTTC AGCCTTAGTT GAAATTGAGA AAAACAGTCC ACACCGGTTT 
GAGTTTTTTA TGGGTCTTTC TGGAGATCAG CCTATTTGTG CTCGAACAGC ATGGGCCTAT 
GGGAAGGCTG GTGGCCGAAT TGCTCATGCT TTGTCAGTGT ACAGTTGTCA ACTTCGTCAA 
CCAAACCAGG TTAAGAAAAT TTTCGAGTTC CTTAAAAATG GCTTCCATGA AGTAAGCAGT 
TCATTGGATT TATCTTTTGA GGATGATTCC GTTGCTGATG AGAAGATTCC ATTCCTAGCT 
TTTCTTGCTA GTGAATTGAA AGAAAAATCA TGTTTCCCCT ATGAATCACC AGCAGGAAGC 
ATATATTTCC GTAATCTAAT TGCAGGCTTT TTGAAGATAT ACCACCATAT TCCACTGAAA 
TCCAATAATG TCGTCATCTT TCCTTCAAGG GCTGTCGCAA TTGAACACAC TCTTCGATTG 
TTCTCACCTC GCGTTGCAAT TGTTGATGAA CATCTGACTC GACACCTACC CCGACAGTGG 
TTGACATCTT TAGCAATTGA GGGTACGGAA AATTATGACC CTTCAAAGGA TGTAATTACA 
GTCATTGATG CACCAAGGCA ATCTGATTTA ATGGTAGAGC TGATAAAGAA GCTCAAGCCA 
CAGGTGGTGA TTACTGGGAT GGCTCAATTT GAGGCTGTAA CCAGTTCAGC TTTTGTTCAG 
CTCTTGGATG TTACTAGGGA AATTGGATCT CGTCTTTTCT TAGACATATC TGATCACCTG 
GAACTCTCCA GCCTTCCCAG TCCCAATGGA GTTCTAAAAT ATCTTGCAGG AACTCGTCTA 
CCTTCACATG CTGCAATCCT ATGTGGTTTT GTAAAGAACA AGGTTTATTC AGATTTGGAA 
GTGGCTTTTG TCATTTCAGA AGAGGAGGCA GTTTTTAAGG CCTTGTCCAA GACTGTAGAA 
GTGCTAGAAG GAAATACTGC ACCAATCAGG CAGTTGTATT ATGGTTGCTT GTTTCATGAG 
CTTCTAGCTT TTCAGCTTGT TGACCGACAT CCGCTTCCAG AGAGGGATTT TGAAAAAGTC 
AAATCAGTTG AGGCGATTGG CTTTGCTAGT TCTGCAATTC CAGTTCTCAA TGATTCAGAG 
TTGTCAATAA GTGAGGAAGA AAAGTCTTCC TTAATCCACA TGGATATAGA TCAAAGTTTT 
ATGCCCATTC CATCTCCTGT GAAGGCTGCA ATATTTGAAA GTTTTGCAAG ACAGAACATG 
GCTGAGTCAG AAATTGATGT CACCCCAAGC ATCAAACAGT TCATCAAGAA CAATTATGGG 
TTTCCTATGG ACAATAAAAC AGAGTTTGTA TATGCTGACT TCTCACAAGC CCTGTTTAAT 
AGACTGATTC TTTGCTGCAT CCAAGAAGGC GGAACATTTT GTTTCCCTGC TGGCTCAAAC 
GGCAATTATG TCTCTGCCGC CAAGTTCTTA AAAGCAAATG TCATGAGTAT CCCTACTGAC 
TCTGGATCTG GCTTCAAACT GACAGACAAG CTACTTGATG GAGTACTTGA TACCGTGAAT 
AAACCATGGG TGTATATTTC TGGTCCAACA ATTACCCCTA CAGGCTTGCT TTACAGCAAT 
AAAGAGATGG AGAACATACT AACTACCTGT GCAAGATTTG GGGCCAGAGT AATCATTGAT 
ACTTCCTTTT CAGGATTGGA GTTTGAAGGT TGGGGTGGTT GGAATTTGGA GACAACATCA 
TCGAAGTTGA ATTCCTCTAA CAATCCATCT TTTTGTGTCT CTCTGATTGG AGGACTGTCT 
CTGAAGCTCT CAAGCGGAGT ACTCAAATTT GGTTATTTGG TTTTAAACGA TCCATTTTTG 
GTTGATGCAT TTTATAGCTT TCCAGGACTA AGCAAACCCC ATAGCACTGT TAAGTATGCT 
ATAAAGAAGC TGTTGAGTCT GAATGAGCAG AAAGCAAGAG ACCTGACAGA TGCTGTTGCA 
GAACAAACAA GAAATTTGAA ATCCAGATCC CAGCGCATGA AAGAGACACT AGAGAAATGC 
GGGTGGGATG TGTTGGAACC TCGTGGCGGG GTGTCCATGA TTGCAAAGCC TTCTGCCTAT 
CTAAACAAGG TTGTTAAGAT CAAGCATTCA CCTGAAAATG ATGAGGAAAA TTCCACTGCT 
TATGAAGTCA AGCTGGATGA CTCAAATATC AGGGAAGCCA TTGTCAGGTC CACAGGTTTG 
TGTATCAACA GTGGTGTATG GACTGGAATT CCTGGTTACT GCCGCTTCAC AATTGCTCTG 
GAAGAACGAG ACTTCGAGCG AGCATTAAAT TGCATAATCA AGTTTAAGGA TCTCATTGGC 
AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC8276899 ( XM_025158568.1 ) cDNA ORF clone, Ricinus communis(castor bean) -> XP_025014336.1 Ricinus communis methionine S-methyltransferase (LOC8276899), mRNA.

Price & Availability↑

CloneID	ORb53478
Clone ID Related Accession (Same CDS sequence)	XM_025158568.1
Accession Version	XM_025158568.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3141bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526918400000
Organism	Ricinus communis(castor bean)
Product	methionine S-methyltransferase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_002994479.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ricinus communis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 2% of CDS bases ##RefSeq-Attributes-END##

ATGATTCTTC TTAGCTCATT ATATTCGGGC AAAACCCCGA CATATTGGTC TCAATCATTA 
CGGAATCTCA ATAATGGTTG TCTATTTGAA ACTTATCTGT CTAGTGCAGG TTATCAGGGG 
AGAAAGAAAT TGACAATGAT GGTGATTCCT AGCATTTTTA TCCCTGAAGA CTGGTCGTTC 
ACTTTTTATG AGGGACTGAA TAGGCATCCC GATTCAATTT TCAAGGACAA GACGGTTGCT 
GAACTTGGTT GTGGAAATGG ATGGATATCA ATTGCTATTG CTGATAAGTG GTTGCCTTCT 
AAGGTGTATG GTCTTGATAT TAATCCTAGA GCAGTCAAGG TTTCTTGGAT AAACTTATAC 
TTAAATGCTT TGGATGAAAA TGGTCAACCC ATCTATGATG CGGAAAAGAA AACTTTACTG 
GACAGGGTGC AGTTTCATGA GTCTGATCTA CTTTCTTATT GTAGAGATCA TGACATTCAA 
CTTGAACGAA TTGTTGGATG CATACCTCAG ATTCTTAACC CAAATCCAGA TGCAATGTCT 
AAAATGATTA CAGAAAATGC AAGTGAAGAG TTTCTGTATT CATTGAGTAA CTACTGTGCA 
CTTCAGGGGT TTGTTGAGGA TCAATTTGGC CTTGGACTAA TTGCCAGGGC TGTTGAGGAA 
GGAATATCTG TCATCAAACC TATGGGGATT ATGATCTTTA ATATGGGCGG CCGTCCAGGA 
CAAGCTGTTT GTAAACGCTT GTTTGAAAGG CGTGGATTTC ATGTAAACAA GCTTTGGCAG 
ACTAAAGTTA TTCAGGCTGC TGACACAGAC ATTTCAGCCT TAGTTGAAAT TGAGAAAAAC 
AGTCCACACC GGTTTGAGTT TTTTATGGGT CTTTCTGGAG ATCAGCCTAT TTGTGCTCGA 
ACAGCATGGG CCTATGGGAA GGCTGGTGGC CGAATTGCTC ATGCTTTGTC AGTGTACAGT 
TGTCAACTTC GTCAACCAAA CCAGGTTAAG AAAATTTTCG AGTTCCTTAA AAATGGCTTC 
CATGAAGTAA GCAGTTCATT GGATTTATCT TTTGAGGATG ATTCCGTTGC TGATGAGAAG 
ATTCCATTCC TAGCTTTTCT TGCTAGTGAA TTGAAAGAAA AATCATGTTT CCCCTATGAA 
TCACCAGCAG GAAGCATATA TTTCCGTAAT CTAATTGCAG GCTTTTTGAA GATATACCAC 
CATATTCCAC TGAAATCCAA TAATGTCGTC ATCTTTCCTT CAAGGGCTGT CGCAATTGAA 
CACACTCTTC GATTGTTCTC ACCTCGCGTT GCAATTGTTG ATGAACATCT GACTCGACAC 
CTACCCCGAC AGTGGTTGAC ATCTTTAGCA ATTGAGGGTA CGGAAAATTA TGACCCTTCA 
AAGGATGTAA TTACAGTCAT TGATGCACCA AGGCAATCTG ATTTAATGGT AGAGCTGATA 
AAGAAGCTCA AGCCACAGGT GGTGATTACT GGGATGGCTC AATTTGAGGC TGTAACCAGT 
TCAGCTTTTG TTCAGCTCTT GGATGTTACT AGGGAAATTG GATCTCGTCT TTTCTTAGAC 
ATATCTGATC ACCTGGAACT CTCCAGCCTT CCCAGTCCCA ATGGAGTTCT AAAATATCTT 
GCAGGAACTC GTCTACCTTC ACATGCTGCA ATCCTATGTG GTTTTGTAAA GAACAAGGTT 
TATTCAGATT TGGAAGTGGC TTTTGTCATT TCAGAAGAGG AGGCAGTTTT TAAGGCCTTG 
TCCAAGACTG TAGAAGTGCT AGAAGGAAAT ACTGCACCAA TCAGGCAGTT GTATTATGGT 
TGCTTGTTTC ATGAGCTTCT AGCTTTTCAG CTTGTTGACC GACATCCGCT TCCAGAGAGG 
GATTTTGAAA AAGTCAAATC AGTTGAGGCG ATTGGCTTTG CTAGTTCTGC AATTCCAGTT 
CTCAATGATT CAGAGTTGTC AATAAGTGAG GAAGAAAAGT CTTCCTTAAT CCACATGGAT 
ATAGATCAAA GTTTTATGCC CATTCCATCT CCTGTGAAGG CTGCAATATT TGAAAGTTTT 
GCAAGACAGA ACATGGCTGA GTCAGAAATT GATGTCACCC CAAGCATCAA ACAGTTCATC 
AAGAACAATT ATGGGTTTCC TATGGACAAT AAAACAGAGT TTGTATATGC TGACTTCTCA 
CAAGCCCTGT TTAATAGACT GATTCTTTGC TGCATCCAAG AAGGCGGAAC ATTTTGTTTC 
CCTGCTGGCT CAAACGGCAA TTATGTCTCT GCCGCCAAGT TCTTAAAAGC AAATGTCATG 
AGTATCCCTA CTGACTCTGG ATCTGGCTTC AAACTGACAG ACAAGCTACT TGATGGAGTA 
CTTGATACCG TGAATAAACC ATGGGTGTAT ATTTCTGGTC CAACAATTAC CCCTACAGGC 
TTGCTTTACA GCAATAAAGA GATGGAGAAC ATACTAACTA CCTGTGCAAG ATTTGGGGCC 
AGAGTAATCA TTGATACTTC CTTTTCAGGA TTGGAGTTTG AAGGTTGGGG TGGTTGGAAT 
TTGGAGACAA CATCATCGAA GTTGAATTCC TCTAACAATC CATCTTTTTG TGTCTCTCTG 
ATTGGAGGAC TGTCTCTGAA GCTCTCAAGC GGAGTACTCA AATTTGGTTA TTTGGTTTTA 
AACGATCCAT TTTTGGTTGA TGCATTTTAT AGCTTTCCAG GACTAAGCAA ACCCCATAGC 
ACTGTTAAGT ATGCTATAAA GAAGCTGTTG AGTCTGAATG AGCAGAAAGC AAGAGACCTG 
ACAGATGCTG TTGCAGAACA AACAAGAAAT TTGAAATCCA GATCCCAGCG CATGAAAGAG 
ACACTAGAGA AATGCGGGTG GGATGTGTTG GAACCTCGTG GCGGGGTGTC CATGATTGCA 
AAGCCTTCTG CCTATCTAAA CAAGGTTGTT AAGATCAAGC ATTCACCTGA AAATGATGAG 
GAAAATTCCA CTGCTTATGA AGTCAAGCTG GATGACTCAA ATATCAGGGA AGCCATTGTC 
AGGTCCACAG GTTTGTGTAT CAACAGTGGT GTATGGACTG GAATTCCTGG TTACTGCCGC 
TTCACAATTG CTCTGGAAGA ACGAGACTTC GAGCGAGCAT TAAATTGCAT AATCAAGTTT 
AAGGATCTCA TTGGCAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025014336.1
CDS	1..3141
Translation	MILLSSLYSGKTPTYWSQSLRNLNNGCLFETYLSSAGYQGRKKLTMMVIPSIFIPEDWSFTFYEGLNRHPDSIFKDKTVAELGCGNGWISIAIADKWLPSKVYGLDINPRAVKVSWINLYLNALDENGQPIYDAEKKTLLDRVQFHESDLLSYCRDHDIQLERIVGCIPQILNPNPDAMSKMITENASEEFLYSLSNYCALQGFVEDQFGLGLIARAVEEGISVIKPMGIMIFNMGGRPGQAVCKRLFERRGFHVNKLWQTKVIQAADTDISALVEIEKNSPHRFEFFMGLSGDQPICARTAWAYGKAGGRIAHALSVYSCQLRQPNQVKKIFEFLKNGFHEVSSSLDLSFEDDSVADEKIPFLAFLASELKEKSCFPYESPAGSIYFRNLIAGFLKIYHHIPLKSNNVVIFPSRAVAIEHTLRLFSPRVAIVDEHLTRHLPRQWLTSLAIEGTENYDPSKDVITVIDAPRQSDLMVELIKKLKPQVVITGMAQFEAVTSSAFVQLLDVTREIGSRLFLDISDHLELSSLPSPNGVLKYLAGTRLPSHAAILCGFVKNKVYSDLEVAFVISEEEAVFKALSKTVEVLEGNTAPIRQLYYGCLFHELLAFQLVDRHPLPERDFEKVKSVEAIGFASSAIPVLNDSELSISEEEKSSLIHMDIDQSFMPIPSPVKAAIFESFARQNMAESEIDVTPSIKQFIKNNYGFPMDNKTEFVYADFSQALFNRLILCCIQEGGTFCFPAGSNGNYVSAAKFLKANVMSIPTDSGSGFKLTDKLLDGVLDTVNKPWVYISGPTITPTGLLYSNKEMENILTTCARFGARVIIDTSFSGLEFEGWGGWNLETTSSKLNSSNNPSFCVSLIGGLSLKLSSGVLKFGYLVLNDPFLVDAFYSFPGLSKPHSTVKYAIKKLLSLNEQKARDLTDAVAEQTRNLKSRSQRMKETLEKCGWDVLEPRGGVSMIAKPSAYLNKVVKIKHSPENDEENSTAYEVKLDDSNIREAIVRSTGLCINSGVWTGIPGYCRFTIALEERDFERALNCIIKFKDLIGN

Target ORF information:

RefSeq Version	XM_025158568.1
Organism	Ricinus communis(castor bean)
Definition	Ricinus communis methionine S-methyltransferase (LOC8276899), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025158568.1

ORF Insert Sequence:

ATGATTCTTC TTAGCTCATT ATATTCGGGC AAAACCCCGA CATATTGGTC TCAATCATTA 
CGGAATCTCA ATAATGGTTG TCTATTTGAA ACTTATCTGT CTAGTGCAGG TTATCAGGGG 
AGAAAGAAAT TGACAATGAT GGTGATTCCT AGCATTTTTA TCCCTGAAGA CTGGTCGTTC 
ACTTTTTATG AGGGACTGAA TAGGCATCCC GATTCAATTT TCAAGGACAA GACGGTTGCT 
GAACTTGGTT GTGGAAATGG ATGGATATCA ATTGCTATTG CTGATAAGTG GTTGCCTTCT 
AAGGTGTATG GTCTTGATAT TAATCCTAGA GCAGTCAAGG TTTCTTGGAT AAACTTATAC 
TTAAATGCTT TGGATGAAAA TGGTCAACCC ATCTATGATG CGGAAAAGAA AACTTTACTG 
GACAGGGTGC AGTTTCATGA GTCTGATCTA CTTTCTTATT GTAGAGATCA TGACATTCAA 
CTTGAACGAA TTGTTGGATG CATACCTCAG ATTCTTAACC CAAATCCAGA TGCAATGTCT 
AAAATGATTA CAGAAAATGC AAGTGAAGAG TTTCTGTATT CATTGAGTAA CTACTGTGCA 
CTTCAGGGGT TTGTTGAGGA TCAATTTGGC CTTGGACTAA TTGCCAGGGC TGTTGAGGAA 
GGAATATCTG TCATCAAACC TATGGGGATT ATGATCTTTA ATATGGGCGG CCGTCCAGGA 
CAAGCTGTTT GTAAACGCTT GTTTGAAAGG CGTGGATTTC ATGTAAACAA GCTTTGGCAG 
ACTAAAGTTA TTCAGGCTGC TGACACAGAC ATTTCAGCCT TAGTTGAAAT TGAGAAAAAC 
AGTCCACACC GGTTTGAGTT TTTTATGGGT CTTTCTGGAG ATCAGCCTAT TTGTGCTCGA 
ACAGCATGGG CCTATGGGAA GGCTGGTGGC CGAATTGCTC ATGCTTTGTC AGTGTACAGT 
TGTCAACTTC GTCAACCAAA CCAGGTTAAG AAAATTTTCG AGTTCCTTAA AAATGGCTTC 
CATGAAGTAA GCAGTTCATT GGATTTATCT TTTGAGGATG ATTCCGTTGC TGATGAGAAG 
ATTCCATTCC TAGCTTTTCT TGCTAGTGAA TTGAAAGAAA AATCATGTTT CCCCTATGAA 
TCACCAGCAG GAAGCATATA TTTCCGTAAT CTAATTGCAG GCTTTTTGAA GATATACCAC 
CATATTCCAC TGAAATCCAA TAATGTCGTC ATCTTTCCTT CAAGGGCTGT CGCAATTGAA 
CACACTCTTC GATTGTTCTC ACCTCGCGTT GCAATTGTTG ATGAACATCT GACTCGACAC 
CTACCCCGAC AGTGGTTGAC ATCTTTAGCA ATTGAGGGTA CGGAAAATTA TGACCCTTCA 
AAGGATGTAA TTACAGTCAT TGATGCACCA AGGCAATCTG ATTTAATGGT AGAGCTGATA 
AAGAAGCTCA AGCCACAGGT GGTGATTACT GGGATGGCTC AATTTGAGGC TGTAACCAGT 
TCAGCTTTTG TTCAGCTCTT GGATGTTACT AGGGAAATTG GATCTCGTCT TTTCTTAGAC 
ATATCTGATC ACCTGGAACT CTCCAGCCTT CCCAGTCCCA ATGGAGTTCT AAAATATCTT 
GCAGGAACTC GTCTACCTTC ACATGCTGCA ATCCTATGTG GTTTTGTAAA GAACAAGGTT 
TATTCAGATT TGGAAGTGGC TTTTGTCATT TCAGAAGAGG AGGCAGTTTT TAAGGCCTTG 
TCCAAGACTG TAGAAGTGCT AGAAGGAAAT ACTGCACCAA TCAGGCAGTT GTATTATGGT 
TGCTTGTTTC ATGAGCTTCT AGCTTTTCAG CTTGTTGACC GACATCCGCT TCCAGAGAGG 
GATTTTGAAA AAGTCAAATC AGTTGAGGCG ATTGGCTTTG CTAGTTCTGC AATTCCAGTT 
CTCAATGATT CAGAGTTGTC AATAAGTGAG GAAGAAAAGT CTTCCTTAAT CCACATGGAT 
ATAGATCAAA GTTTTATGCC CATTCCATCT CCTGTGAAGG CTGCAATATT TGAAAGTTTT 
GCAAGACAGA ACATGGCTGA GTCAGAAATT GATGTCACCC CAAGCATCAA ACAGTTCATC 
AAGAACAATT ATGGGTTTCC TATGGACAAT AAAACAGAGT TTGTATATGC TGACTTCTCA 
CAAGCCCTGT TTAATAGACT GATTCTTTGC TGCATCCAAG AAGGCGGAAC ATTTTGTTTC 
CCTGCTGGCT CAAACGGCAA TTATGTCTCT GCCGCCAAGT TCTTAAAAGC AAATGTCATG 
AGTATCCCTA CTGACTCTGG ATCTGGCTTC AAACTGACAG ACAAGCTACT TGATGGAGTA 
CTTGATACCG TGAATAAACC ATGGGTGTAT ATTTCTGGTC CAACAATTAC CCCTACAGGC 
TTGCTTTACA GCAATAAAGA GATGGAGAAC ATACTAACTA CCTGTGCAAG ATTTGGGGCC 
AGAGTAATCA TTGATACTTC CTTTTCAGGA TTGGAGTTTG AAGGTTGGGG TGGTTGGAAT 
TTGGAGACAA CATCATCGAA GTTGAATTCC TCTAACAATC CATCTTTTTG TGTCTCTCTG 
ATTGGAGGAC TGTCTCTGAA GCTCTCAAGC GGAGTACTCA AATTTGGTTA TTTGGTTTTA 
AACGATCCAT TTTTGGTTGA TGCATTTTAT AGCTTTCCAG GACTAAGCAA ACCCCATAGC 
ACTGTTAAGT ATGCTATAAA GAAGCTGTTG AGTCTGAATG AGCAGAAAGC AAGAGACCTG 
ACAGATGCTG TTGCAGAACA AACAAGAAAT TTGAAATCCA GATCCCAGCG CATGAAAGAG 
ACACTAGAGA AATGCGGGTG GGATGTGTTG GAACCTCGTG GCGGGGTGTC CATGATTGCA 
AAGCCTTCTG CCTATCTAAA CAAGGTTGTT AAGATCAAGC ATTCACCTGA AAATGATGAG 
GAAAATTCCA CTGCTTATGA AGTCAAGCTG GATGACTCAA ATATCAGGGA AGCCATTGTC 
AGGTCCACAG GTTTGTGTAT CAACAGTGGT GTATGGACTG GAATTCCTGG TTACTGCCGC 
TTCACAATTG CTCTGGAAGA ACGAGACTTC GAGCGAGCAT TAAATTGCAT AATCAAGTTT 
AAGGATCTCA TTGGCAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC8276899 cDNA ORF clone, Ricinus communis(castor bean)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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