Pde2a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pde2a
Entrez Gene ID	81743
Full Name	phosphodiesterase 2A
Synonyms	CGS-PDE,Pde2,Pde2a2
General protein information	Preferred Names phosphodiesterase 2A Names cGMP-dependent 3',5'-cyclic phosphodiesterase cyclic GMP-stimulated phosphodiesterase phosphodiesterase 2A, cGMP-stimulated
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q32
Summary	activation induces decreased cAMP accumulation; involved in nitric oxide mediated signaling in cardiac fibroblasts [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_031079.1	NP_112341.1	cGMP-dependent 3',5'-cyclic phosphodiesterase isoform PDE2A2 precursor
NM_001143847.1	NP_001137319.1	cGMP-dependent 3',5'-cyclic phosphodiesterase isoform PDE2A3
NM_001270604.2	NP_001257533.1	cGMP-dependent 3',5'-cyclic phosphodiesterase isoform PDE2A1
NM_001143847.2	NP_001137319.1	cGMP-dependent 3',5'-cyclic phosphodiesterase isoform PDE2A3
NM_031079.2	NP_112341.1	cGMP-dependent 3',5'-cyclic phosphodiesterase isoform PDE2A2 precursor
NM_001270604.1	NP_001257533.1	cGMP-dependent 3',5'-cyclic phosphodiesterase isoform PDE2A1

Pathways from BioSystems

Homologies

Homo sapiens (human)	PDE2A	NP_001139681.1
Macaca mulatta (Rhesus monkey)	PDE2A	NP_001124298.1
Xenopus tropicalis (tropical clawed frog)	pde2a	NP_001072607.1
Pan troglodytes (chimpanzee)	PDE2A	XP_001174746.1
Bos taurus (cattle)	PDE2A	NP_001137318.1
Mus musculus (house mouse)	Pde2a	NP_001137320.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008304	XP_317162.4
Rattus norvegicus (Norway rat)	Pde2a	NP_001137319.1
Canis lupus familiaris (dog)	PDE2A	XP_851951.2

Gene Ontology
Bibliography

Related articles in PubMed

PDE2A2 regulates mitochondria morphology and apoptotic cell death via local modulation of cAMP/PKA signalling.
Monterisi S, Lobo MJ, Livie C, Castle JC, Weinberger M, Baillie G, Surdo NC, Musheshe N, Stangherlin A, Gottlieb E, Maizels R, Bortolozzi M, Micaroni M, Zaccolo M
eLife6(2017 05)

PDE2 activity differs in right and left rat ventricular myocardium and differentially regulates β2 adrenoceptor-mediated effects.
Soler F, Fernández-Belda F, Pérez-Schindler J, Handschin C, Fuente T, Hernandez-Cascales J
Experimental biology and medicine (Maywood, N.J.)240(9)1205-13(2015 Sep)

Cardiac Hypertrophy Is Inhibited by a Local Pool of cAMP Regulated by Phosphodiesterase 2.
Zoccarato A, Surdo NC, Aronsen JM, Fields LA, Mancuso L, Dodoni G, Stangherlin A, Livie C, Jiang H, Sin YY, Gesellchen F, Terrin A, Baillie GS, Nicklin SA, Graham D, Szabo-Fresnais N, Krall J, Vandeput F, Movsesian M, Furlan L, Corsetti V, Hamilton G, Lefkimmiatis K, Sjaastad I, Zaccolo M
Circulation research117(8)707-19(2015 Sep)

Efficacy of B-Type Natriuretic Peptide Is Coupled to Phosphodiesterase 2A in Cardiac Sympathetic Neurons.
Li D, Lu CJ, Hao G, Wright H, Woodward L, Liu K, Vergari E, Surdo NC, Herring N, Zaccolo M, Paterson DJ
Hypertension (Dallas, Tex. : 1979)66(1)190-8(2015 Jul)

Global genetic analysis in mice unveils central role for cilia in congenital heart disease.
Li Y, Klena NT, Gabriel GC, Liu X, Kim AJ, Lemke K, Chen Y, Chatterjee B, Devine W, Damerla RR, Chang C, Yagi H, San Agustin JT, Thahir M, Anderton S, Lawhead C, Vescovi A, Pratt H, Morgan J, Haynes L, Smith CL, Eppig JT, Reinholdt L, Francis R, Leatherbury L, Ganapathiraju MK, Tobita K, Pazour GJ, Lo CW
Nature521(7553)520-4(2015 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Pde2a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pde2a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa04372	NM_001143847.1 Latest version!	Rattus norvegicus phosphodiesterase 2A (Pde2a), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ORa07535	NM_031079.1 Latest version!	Rattus norvegicus phosphodiesterase 2A (Pde2a), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ORa09386	NM_001270604.2 Latest version!	Rattus norvegicus phosphodiesterase 2A (Pde2a), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ORa63305	NM_001143847.2 Latest version!	Rattus norvegicus phosphodiesterase 2A (Pde2a), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ORa63312	NM_031079.2 Latest version!	Rattus norvegicus phosphodiesterase 2A (Pde2a), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
ORa09386	NM_001270604.1	Rattus norvegicus phosphodiesterase 2A (Pde2a), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pde2a ( NM_001143847.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001137319.1 Rattus norvegicus phosphodiesterase 2A (Pde2a), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ORa04372
Clone ID Related Accession (Same CDS sequence)	NM_001143847.1
Accession Version	NM_001143847.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2808bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1506787200000
Organism	Rattus norvegicus(Norway rat)
Product	cGMP-dependent 3',5'-cyclic phosphodiesterase isoform PDE2A3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CB764775.1 and U21101.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (PDE2A3). This variant is assembled from partial rat transcripts. The full-length exon combination is based on the mouse ortholog, NM_001008548.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGGGCAGG CATGCGGCCA CTCCATCCTC TGCAGGAGCC AGCAGTACCC GGCAGCGCGA 
CCGGCTGAGC CGCGGGGCCA GCAGGTCTTC CTCAAGCCGG ACGAACCGCC GCCGCAGCCA 
TGCGCCGACA GCCTGCAGGA TGCTTTGCTG AGCCTGGGCG CCGTTATCGA CATCGCGGGC 
CTTCGACAGG CTGCCAAGGA TGCCCTCTCG GCCGTGCTCC CCAAAGTGGA GACTGTCTAC 
ACCTACCTGG TAGATGGGGA GTCCAGGCTG GTGTGTGAGG ACCCCCCTCA TGAGCTGCCA 
CAGGAAGGAA AAATTCGAGA GGCTGTGATT TCTCGGAAGC GGCTGAGCTG CGATGGGCTG 
GGACCTTCAG ACCTACTAGG AAAGCCCTTG GCCAGGCTGG TGGCTCCACT GGCCCCTGAC 
ACGCAAGTGC TGGTCATACC CCTGCTGGAC AAGGAGACTG GAACTGTGGC AGCCGTCATC 
TTGGTGCACT GTGGCCAGCT GAGTGACAGT GAGGAGCAGA GCCTGCAGGT GGTGGAGAAG 
CATGCTCTGG TGGCCCTGCA GAGGGTGCAG GCCCTGCAGC AGCGCAGGCC TGAAGCTGTT 
CAGAACACCT CAGCGGATCC CTCTGAAGAT CAAAAGGATG AGAAGGGATA CACCGCCCAT 
GACCGAAAGA TCCTGCAACT GTGTGGAGAA CTCTATGACT TGGATGCCAC TTCTCTGCAG 
CTCAAAGTCC TTCGATATCT TCAGCAGGAG ACACAGGCCA CTCACTGCTG CCTCCTGCTG 
GTGTCAGAGG ACAACCTGCA GCTTTCCTGC AAGGTCATTG GAGAGAAAGT GCTGGGAGAA 
GAGGTCAGCT TTCCATTGAC CATGGGACGC CTGGGCCAGG TGGTGGAGGA CAAACAGTGT 
ATCCAGTTGA AGGACCTAAC CTCTGACGAT GTGCAACAGC TACAAAACAT GTTGGGCTGT 
GAGCTTCGGG CTATGCTATG TGTCCCTGTC ATCAGTCGAG CCACTGACCA GGTGGTGGCC 
CTGGCTTGCG CCTTCAACAA GCTTGGAGGA GACTTCTTCA CAGATGAGGA TGAACGTGCG 
ATCCAACACT GCTTCCACTA CACAGGCACG GTGCTCACCA GTACCTTGGC CTTCCAGAAG 
GAGCAGAAGC TCAAGTGTGA GTGCCAGGCT CTTCTCCAAG TGGCAAAGAA CCTCTTCACC 
CACCTGGATG ACGTCTCTGT CCTGCTACAG GAGATCATCA CAGAGGCCAG AAACCTCAGC 
AACGCAGAGA TCTGCTCCGT GTTCCTGCTG GATCAGAATG AGCTGGTTGC CAAGGTGTTC 
GATGGCGGTG TAGTGGACGA TGAGAGTTAT GAGATCCGCA TCCCTGCGGA CCAAGGCATC 
GCGGGCCACG TGGCGACCAC GGGCCAGATC CTGAACATCC CAGATGCATA CGCCCATCCG 
CTTTTCTATC GCGGCGTAGA CGACAGCACT GGCTTCCGCA CGCGCAACAT TCTCTGCTTC 
CCTATCAAGA ACGAGAACCA AGAGGTCATC GGTGTGGCTG AGCTAGTGAA CAAGATCAAT 
GGGCCATGGT TCAGCAAATT TGATGAGGAC CTGGCCACAG CCTTCTCCAT CTACTGTGGC 
ATTAGCATCG CTCACTCTCT CCTATACAAA AAGGTGAATG AAGCCCAGTA CCGCAGCCAC 
CTGGCCAACG AGATGATGAT GTACCATATG AAGGTCTCTG ACGATGAATA CACCAAACTT 
CTCCATGATG GCATCCAACC TGTGGCCGCC ATTGACTCCA ACTTTGCCAA CTTCACCTAT 
ACTCCTCGGT CTCTGCCTGA GGACGATACT TCTATGGCCA TCCTGAGCAT GCTGCAAGAC 
ATGAACTTCA TCAATAACTA CAAAATTGAC TGCCCAACTC TGGCCCGGTT CTGCCTGATG 
GTGAAGAAAG GCTACCGGGA TCCGCCCTAC CACAACTGGA TGCACGCCTT CTCTGTCTCT 
CACTTTTGCT ACTTGCTCTA CAAGAATCTG GAGCTCTCCA ACTACCTCGA GGACATCGAG 
ATCTTTGCGT TGTTTATTTC CTGCATGTGT CACGACCTGG ACCACAGAGG CACAAACAAC 
TCCTTCCAGG TGGCCTCGAA ATCTGTGCTG GCTGCACTCT ACAGCTCAGA GGGCTCTGTC 
ATGGAGAGGC ACCACTTTGC TCAGGCCATT GCTATCCTCA ACACCCATGG CTGTAATATC 
TTTGACCACT TCTCTCGGAA GGACTATCAG CGAATGCTGG ACCTGATGAG GGACATCATC 
TTGGCCACGG ACCTGGCACA CCACCTCCGC ATCTTCAAGG ACCTGCAGAA GATGGCTGAA 
GTGGGTTATG ACCGAAACAA CAAGCAACAC CACAGGCTTC TTCTATGCCT CCTTATGACC 
TCCTGTGACC TCTCTGACCA GACAAAGGGC TGGAAGACCA CCAGAAAGAT TGCGGAGCTG 
ATCTACAAAG AGTTCTTCTC CCAAGGAGAC TTGGAGAAGG CCATGGGCAA CCGACCAATG 
GAGATGATGG ATCGGGAGAA GGCCTACATC CCTGAACTTC AGATCAGCTT TATGGAGCAC 
ATCGCCATGC CCATCTACAA GCTGTTACAA GACCTGTTCC CCAAGGCAGC AGAGCTGTAT 
GAACGCGTGG CCTCCAATCG TGAGCACTGG ACCAAGGTGT CCCACAAGTT CACCATCCGA 
GGCCTCCCCA GTAACAACTC GCTGGATTTC CTGGATGAGG AATATGAGGT CCCTGATTTG 
GACGTCACCA GGGCTCCTGT CAATGGCTGC TGCAGCCTCG AGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137319.1
CDS	192..2999
Translation	MGQACGHSILCRSQQYPAARPAEPRGQQVFLKPDEPPPQPCADSLQDALLSLGAVIDIAGLRQAAKDALSAVLPKVETVYTYLVDGESRLVCEDPPHELPQEGKIREAVISRKRLSCDGLGPSDLLGKPLARLVAPLAPDTQVLVIPLLDKETGTVAAVILVHCGQLSDSEEQSLQVVEKHALVALQRVQALQQRRPEAVQNTSADPSEDQKDEKGYTAHDRKILQLCGELYDLDATSLQLKVLRYLQQETQATHCCLLLVSEDNLQLSCKVIGEKVLGEEVSFPLTMGRLGQVVEDKQCIQLKDLTSDDVQQLQNMLGCELRAMLCVPVISRATDQVVALACAFNKLGGDFFTDEDERAIQHCFHYTGTVLTSTLAFQKEQKLKCECQALLQVAKNLFTHLDDVSVLLQEIITEARNLSNAEICSVFLLDQNELVAKVFDGGVVDDESYEIRIPADQGIAGHVATTGQILNIPDAYAHPLFYRGVDDSTGFRTRNILCFPIKNENQEVIGVAELVNKINGPWFSKFDEDLATAFSIYCGISIAHSLLYKKVNEAQYRSHLANEMMMYHMKVSDDEYTKLLHDGIQPVAAIDSNFANFTYTPRSLPEDDTSMAILSMLQDMNFINNYKIDCPTLARFCLMVKKGYRDPPYHNWMHAFSVSHFCYLLYKNLELSNYLEDIEIFALFISCMCHDLDHRGTNNSFQVASKSVLAALYSSEGSVMERHHFAQAIAILNTHGCNIFDHFSRKDYQRMLDLMRDIILATDLAHHLRIFKDLQKMAEVGYDRNNKQHHRLLLCLLMTSCDLSDQTKGWKTTRKIAELIYKEFFSQGDLEKAMGNRPMEMMDREKAYIPELQISFMEHIAMPIYKLLQDLFPKAAELYERVASNREHWTKVSHKFTIRGLPSNNSLDFLDEEYEVPDLDVTRAPVNGCCSLEG

Target ORF information:

RefSeq Version	NM_001143847.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosphodiesterase 2A (Pde2a), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001143847.1

ORF Insert Sequence:

ATGGGGCAGG CATGCGGCCA CTCCATCCTC TGCAGGAGCC AGCAGTACCC GGCAGCGCGA 
CCGGCTGAGC CGCGGGGCCA GCAGGTCTTC CTCAAGCCGG ACGAACCGCC GCCGCAGCCA 
TGCGCCGACA GCCTGCAGGA TGCTTTGCTG AGCCTGGGCG CCGTTATCGA CATCGCGGGC 
CTTCGACAGG CTGCCAAGGA TGCCCTCTCG GCCGTGCTCC CCAAAGTGGA GACTGTCTAC 
ACCTACCTGG TAGATGGGGA GTCCAGGCTG GTGTGTGAGG ACCCCCCTCA TGAGCTGCCA 
CAGGAAGGAA AAATTCGAGA GGCTGTGATT TCTCGGAAGC GGCTGAGCTG CGATGGGCTG 
GGACCTTCAG ACCTACTAGG AAAGCCCTTG GCCAGGCTGG TGGCTCCACT GGCCCCTGAC 
ACGCAAGTGC TGGTCATACC CCTGCTGGAC AAGGAGACTG GAACTGTGGC AGCCGTCATC 
TTGGTGCACT GTGGCCAGCT GAGTGACAGT GAGGAGCAGA GCCTGCAGGT GGTGGAGAAG 
CATGCTCTGG TGGCCCTGCA GAGGGTGCAG GCCCTGCAGC AGCGCAGGCC TGAAGCTGTT 
CAGAACACCT CAGCGGATCC CTCTGAAGAT CAAAAGGATG AGAAGGGATA CACCGCCCAT 
GACCGAAAGA TCCTGCAACT GTGTGGAGAA CTCTATGACT TGGATGCCAC TTCTCTGCAG 
CTCAAAGTCC TTCGATATCT TCAGCAGGAG ACACAGGCCA CTCACTGCTG CCTCCTGCTG 
GTGTCAGAGG ACAACCTGCA GCTTTCCTGC AAGGTCATTG GAGAGAAAGT GCTGGGAGAA 
GAGGTCAGCT TTCCATTGAC CATGGGACGC CTGGGCCAGG TGGTGGAGGA CAAACAGTGT 
ATCCAGTTGA AGGACCTAAC CTCTGACGAT GTGCAACAGC TACAAAACAT GTTGGGCTGT 
GAGCTTCGGG CTATGCTATG TGTCCCTGTC ATCAGTCGAG CCACTGACCA GGTGGTGGCC 
CTGGCTTGCG CCTTCAACAA GCTTGGAGGA GACTTCTTCA CAGATGAGGA TGAACGTGCG 
ATCCAACACT GCTTCCACTA CACAGGCACG GTGCTCACCA GTACCTTGGC CTTCCAGAAG 
GAGCAGAAGC TCAAGTGTGA GTGCCAGGCT CTTCTCCAAG TGGCAAAGAA CCTCTTCACC 
CACCTGGATG ACGTCTCTGT CCTGCTACAG GAGATCATCA CAGAGGCCAG AAACCTCAGC 
AACGCAGAGA TCTGCTCCGT GTTCCTGCTG GATCAGAATG AGCTGGTTGC CAAGGTGTTC 
GATGGCGGTG TAGTGGACGA TGAGAGTTAT GAGATCCGCA TCCCTGCGGA CCAAGGCATC 
GCGGGCCACG TGGCGACCAC GGGCCAGATC CTGAACATCC CAGATGCATA CGCCCATCCG 
CTTTTCTATC GCGGCGTAGA CGACAGCACT GGCTTCCGCA CGCGCAACAT TCTCTGCTTC 
CCTATCAAGA ACGAGAACCA AGAGGTCATC GGTGTGGCTG AGCTAGTGAA CAAGATCAAT 
GGGCCATGGT TCAGCAAATT TGATGAGGAC CTGGCCACAG CCTTCTCCAT CTACTGTGGC 
ATTAGCATCG CTCACTCTCT CCTATACAAA AAGGTGAATG AAGCCCAGTA CCGCAGCCAC 
CTGGCCAACG AGATGATGAT GTACCATATG AAGGTCTCTG ACGATGAATA CACCAAACTT 
CTCCATGATG GCATCCAACC TGTGGCCGCC ATTGACTCCA ACTTTGCCAA CTTCACCTAT 
ACTCCTCGGT CTCTGCCTGA GGACGATACT TCTATGGCCA TCCTGAGCAT GCTGCAAGAC 
ATGAACTTCA TCAATAACTA CAAAATTGAC TGCCCAACTC TGGCCCGGTT CTGCCTGATG 
GTGAAGAAAG GCTACCGGGA TCCGCCCTAC CACAACTGGA TGCACGCCTT CTCTGTCTCT 
CACTTTTGCT ACTTGCTCTA CAAGAATCTG GAGCTCTCCA ACTACCTCGA GGACATCGAG 
ATCTTTGCGT TGTTTATTTC CTGCATGTGT CACGACCTGG ACCACAGAGG CACAAACAAC 
TCCTTCCAGG TGGCCTCGAA ATCTGTGCTG GCTGCACTCT ACAGCTCAGA GGGCTCTGTC 
ATGGAGAGGC ACCACTTTGC TCAGGCCATT GCTATCCTCA ACACCCATGG CTGTAATATC 
TTTGACCACT TCTCTCGGAA GGACTATCAG CGAATGCTGG ACCTGATGAG GGACATCATC 
TTGGCCACGG ACCTGGCACA CCACCTCCGC ATCTTCAAGG ACCTGCAGAA GATGGCTGAA 
GTGGGTTATG ACCGAAACAA CAAGCAACAC CACAGGCTTC TTCTATGCCT CCTTATGACC 
TCCTGTGACC TCTCTGACCA GACAAAGGGC TGGAAGACCA CCAGAAAGAT TGCGGAGCTG 
ATCTACAAAG AGTTCTTCTC CCAAGGAGAC TTGGAGAAGG CCATGGGCAA CCGACCAATG 
GAGATGATGG ATCGGGAGAA GGCCTACATC CCTGAACTTC AGATCAGCTT TATGGAGCAC 
ATCGCCATGC CCATCTACAA GCTGTTACAA GACCTGTTCC CCAAGGCAGC AGAGCTGTAT 
GAACGCGTGG CCTCCAATCG TGAGCACTGG ACCAAGGTGT CCCACAAGTT CACCATCCGA 
GGCCTCCCCA GTAACAACTC GCTGGATTTC CTGGATGAGG AATATGAGGT CCCTGATTTG 
GACGTCACCA GGGCTCCTGT CAATGGCTGC TGCAGCCTCG AGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pde2a ( NM_031079.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_112341.1 Rattus norvegicus phosphodiesterase 2A (Pde2a), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ORa07535
Clone ID Related Accession (Same CDS sequence)	NM_031079.1
Accession Version	NM_031079.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2787bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1514217600000
Organism	Rattus norvegicus(Norway rat)
Product	cGMP-dependent 3',5'-cyclic phosphodiesterase isoform PDE2A2 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U21101.1. Transcript Variant: This variant (2) represents use of an alternate promoter and has a distinct 5' UTR and 5' coding region, compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U21101.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGTCCTGG TGTTGCACCA CATCCTCATC GCTGTTGTCC AATTCCTCAG GCGGGGCCAG 
CAGGTCTTCC TCAAGCCGGA CGAACCGCCG CCGCAGCCAT GCGCCGACAG CCTGCAGGAT 
GCTTTGCTGA GCCTGGGCGC CGTTATCGAC ATCGCGGGCC TTCGACAGGC TGCCAAGGAT 
GCCCTCTCGG CCGTGCTCCC CAAAGTGGAG ACTGTCTACA CCTACCTGGT AGATGGGGAG 
TCCAGGCTGG TGTGTGAGGA CCCCCCTCAT GAGCTGCCAC AGGAAGGAAA AATTCGAGAG 
GCTGTGATTT CTCGGAAGCG GCTGAGCTGC GATGGGCTGG GACCTTCAGA CCTACTAGGA 
AAGCCCTTGG CCAGGCTGGT GGCTCCACTG GCCCCTGACA CGCAAGTGCT GGTCATACCC 
CTGCTGGACA AGGAGACTGG AACTGTGGCA GCCGTCATCT TGGTGCACTG TGGCCAGCTG 
AGTGACAGTG AGGAGCAGAG CCTGCAGGTG GTGGAGAAGC ATGCTCTGGT GGCCCTGCAG 
AGGGTGCAGG CCCTGCAGCA GCGCAGGCCT GAAGCTGTTC AGAACACCTC AGCGGATCCC 
TCTGAAGATC AAAAGGATGA GAAGGGATAC ACCGCCCATG ACCGAAAGAT CCTGCAACTG 
TGTGGAGAAC TCTATGACTT GGATGCCACT TCTCTGCAGC TCAAAGTCCT TCGATATCTT 
CAGCAGGAGA CACAGGCCAC TCACTGCTGC CTCCTGCTGG TGTCAGAGGA CAACCTGCAG 
CTTTCCTGCA AGGTCATTGG AGAGAAAGTG CTGGGAGAAG AGGTCAGCTT TCCATTGACC 
ATGGGACGCC TGGGCCAGGT GGTGGAGGAC AAACAGTGTA TCCAGTTGAA GGACCTAACC 
TCTGACGATG TGCAACAGCT ACAAAACATG TTGGGCTGTG AGCTTCGGGC TATGCTATGT 
GTCCCTGTCA TCAGTCGAGC CACTGACCAG GTGGTGGCCC TGGCTTGCGC CTTCAACAAG 
CTTGGAGGAG ACTTCTTCAC AGATGAGGAT GAACGTGCGA TCCAACACTG CTTCCACTAC 
ACAGGCACGG TGCTCACCAG TACCTTGGCC TTCCAGAAGG AGCAGAAGCT CAAGTGTGAG 
TGCCAGGCTC TTCTCCAAGT GGCAAAGAAC CTCTTCACCC ACCTGGATGA CGTCTCTGTC 
CTGCTACAGG AGATCATCAC AGAGGCCAGA AACCTCAGCA ACGCAGAGAT CTGCTCCGTG 
TTCCTGCTGG ATCAGAATGA GCTGGTTGCC AAGGTGTTCG ATGGCGGTGT AGTGGACGAT 
GAGAGTTATG AGATCCGCAT CCCTGCGGAC CAAGGCATCG CGGGCCACGT GGCGACCACG 
GGCCAGATCC TGAACATCCC AGATGCATAC GCCCATCCGC TTTTCTATCG CGGCGTAGAC 
GACAGCACTG GCTTCCGCAC GCGCAACATT CTCTGCTTCC CTATCAAGAA CGAGAACCAA 
GAGGTCATCG GTGTGGCTGA GCTAGTGAAC AAGATCAATG GGCCATGGTT CAGCAAATTT 
GATGAGGACC TGGCCACAGC CTTCTCCATC TACTGTGGCA TTAGCATCGC TCACTCTCTC 
CTATACAAAA AGGTGAATGA AGCCCAGTAC CGCAGCCACC TGGCCAACGA GATGATGATG 
TACCATATGA AGGTCTCTGA CGATGAATAC ACCAAACTTC TCCATGATGG CATCCAACCT 
GTGGCCGCCA TTGACTCCAA CTTTGCCAAC TTCACCTATA CTCCTCGGTC TCTGCCTGAG 
GACGATACTT CTATGGCCAT CCTGAGCATG CTGCAAGACA TGAACTTCAT CAATAACTAC 
AAAATTGACT GCCCAACTCT GGCCCGGTTC TGCCTGATGG TGAAGAAAGG CTACCGGGAT 
CCGCCCTACC ACAACTGGAT GCACGCCTTC TCTGTCTCTC ACTTTTGCTA CTTGCTCTAC 
AAGAATCTGG AGCTCTCCAA CTACCTCGAG GACATCGAGA TCTTTGCGTT GTTTATTTCC 
TGCATGTGTC ACGACCTGGA CCACAGAGGC ACAAACAACT CCTTCCAGGT GGCCTCGAAA 
TCTGTGCTGG CTGCACTCTA CAGCTCAGAG GGCTCTGTCA TGGAGAGGCA CCACTTTGCT 
CAGGCCATTG CTATCCTCAA CACCCATGGC TGTAATATCT TTGACCACTT CTCTCGGAAG 
GACTATCAGC GAATGCTGGA CCTGATGAGG GACATCATCT TGGCCACGGA CCTGGCACAC 
CACCTCCGCA TCTTCAAGGA CCTGCAGAAG ATGGCTGAAG TGGGTTATGA CCGAAACAAC 
AAGCAACACC ACAGGCTTCT TCTATGCCTC CTTATGACCT CCTGTGACCT CTCTGACCAG 
ACAAAGGGCT GGAAGACCAC CAGAAAGATT GCGGAGCTGA TCTACAAAGA GTTCTTCTCC 
CAAGGAGACT TGGAGAAGGC CATGGGCAAC CGACCAATGG AGATGATGGA TCGGGAGAAG 
GCCTACATCC CTGAACTTCA GATCAGCTTT ATGGAGCACA TCGCCATGCC CATCTACAAG 
CTGTTACAAG ACCTGTTCCC CAAGGCAGCA GAGCTGTATG AACGCGTGGC CTCCAATCGT 
GAGCACTGGA CCAAGGTGTC CCACAAGTTC ACCATCCGAG GCCTCCCCAG TAACAACTCG 
CTGGATTTCC TGGATGAGGA ATATGAGGTC CCTGATTTGG ACGTCACCAG GGCTCCTGTC 
AATGGCTGCT GCAGCCTCGA GGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_112341.1
CDS	38..2824
Translation	MVLVLHHILIAVVQFLRRGQQVFLKPDEPPPQPCADSLQDALLSLGAVIDIAGLRQAAKDALSAVLPKVETVYTYLVDGESRLVCEDPPHELPQEGKIREAVISRKRLSCDGLGPSDLLGKPLARLVAPLAPDTQVLVIPLLDKETGTVAAVILVHCGQLSDSEEQSLQVVEKHALVALQRVQALQQRRPEAVQNTSADPSEDQKDEKGYTAHDRKILQLCGELYDLDATSLQLKVLRYLQQETQATHCCLLLVSEDNLQLSCKVIGEKVLGEEVSFPLTMGRLGQVVEDKQCIQLKDLTSDDVQQLQNMLGCELRAMLCVPVISRATDQVVALACAFNKLGGDFFTDEDERAIQHCFHYTGTVLTSTLAFQKEQKLKCECQALLQVAKNLFTHLDDVSVLLQEIITEARNLSNAEICSVFLLDQNELVAKVFDGGVVDDESYEIRIPADQGIAGHVATTGQILNIPDAYAHPLFYRGVDDSTGFRTRNILCFPIKNENQEVIGVAELVNKINGPWFSKFDEDLATAFSIYCGISIAHSLLYKKVNEAQYRSHLANEMMMYHMKVSDDEYTKLLHDGIQPVAAIDSNFANFTYTPRSLPEDDTSMAILSMLQDMNFINNYKIDCPTLARFCLMVKKGYRDPPYHNWMHAFSVSHFCYLLYKNLELSNYLEDIEIFALFISCMCHDLDHRGTNNSFQVASKSVLAALYSSEGSVMERHHFAQAIAILNTHGCNIFDHFSRKDYQRMLDLMRDIILATDLAHHLRIFKDLQKMAEVGYDRNNKQHHRLLLCLLMTSCDLSDQTKGWKTTRKIAELIYKEFFSQGDLEKAMGNRPMEMMDREKAYIPELQISFMEHIAMPIYKLLQDLFPKAAELYERVASNREHWTKVSHKFTIRGLPSNNSLDFLDEEYEVPDLDVTRAPVNGCCSLEG

Target ORF information:

RefSeq Version	NM_031079.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosphodiesterase 2A (Pde2a), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031079.1

ORF Insert Sequence:

ATGGTCCTGG TGTTGCACCA CATCCTCATC GCTGTTGTCC AATTCCTCAG GCGGGGCCAG 
CAGGTCTTCC TCAAGCCGGA CGAACCGCCG CCGCAGCCAT GCGCCGACAG CCTGCAGGAT 
GCTTTGCTGA GCCTGGGCGC CGTTATCGAC ATCGCGGGCC TTCGACAGGC TGCCAAGGAT 
GCCCTCTCGG CCGTGCTCCC CAAAGTGGAG ACTGTCTACA CCTACCTGGT AGATGGGGAG 
TCCAGGCTGG TGTGTGAGGA CCCCCCTCAT GAGCTGCCAC AGGAAGGAAA AATTCGAGAG 
GCTGTGATTT CTCGGAAGCG GCTGAGCTGC GATGGGCTGG GACCTTCAGA CCTACTAGGA 
AAGCCCTTGG CCAGGCTGGT GGCTCCACTG GCCCCTGACA CGCAAGTGCT GGTCATACCC 
CTGCTGGACA AGGAGACTGG AACTGTGGCA GCCGTCATCT TGGTGCACTG TGGCCAGCTG 
AGTGACAGTG AGGAGCAGAG CCTGCAGGTG GTGGAGAAGC ATGCTCTGGT GGCCCTGCAG 
AGGGTGCAGG CCCTGCAGCA GCGCAGGCCT GAAGCTGTTC AGAACACCTC AGCGGATCCC 
TCTGAAGATC AAAAGGATGA GAAGGGATAC ACCGCCCATG ACCGAAAGAT CCTGCAACTG 
TGTGGAGAAC TCTATGACTT GGATGCCACT TCTCTGCAGC TCAAAGTCCT TCGATATCTT 
CAGCAGGAGA CACAGGCCAC TCACTGCTGC CTCCTGCTGG TGTCAGAGGA CAACCTGCAG 
CTTTCCTGCA AGGTCATTGG AGAGAAAGTG CTGGGAGAAG AGGTCAGCTT TCCATTGACC 
ATGGGACGCC TGGGCCAGGT GGTGGAGGAC AAACAGTGTA TCCAGTTGAA GGACCTAACC 
TCTGACGATG TGCAACAGCT ACAAAACATG TTGGGCTGTG AGCTTCGGGC TATGCTATGT 
GTCCCTGTCA TCAGTCGAGC CACTGACCAG GTGGTGGCCC TGGCTTGCGC CTTCAACAAG 
CTTGGAGGAG ACTTCTTCAC AGATGAGGAT GAACGTGCGA TCCAACACTG CTTCCACTAC 
ACAGGCACGG TGCTCACCAG TACCTTGGCC TTCCAGAAGG AGCAGAAGCT CAAGTGTGAG 
TGCCAGGCTC TTCTCCAAGT GGCAAAGAAC CTCTTCACCC ACCTGGATGA CGTCTCTGTC 
CTGCTACAGG AGATCATCAC AGAGGCCAGA AACCTCAGCA ACGCAGAGAT CTGCTCCGTG 
TTCCTGCTGG ATCAGAATGA GCTGGTTGCC AAGGTGTTCG ATGGCGGTGT AGTGGACGAT 
GAGAGTTATG AGATCCGCAT CCCTGCGGAC CAAGGCATCG CGGGCCACGT GGCGACCACG 
GGCCAGATCC TGAACATCCC AGATGCATAC GCCCATCCGC TTTTCTATCG CGGCGTAGAC 
GACAGCACTG GCTTCCGCAC GCGCAACATT CTCTGCTTCC CTATCAAGAA CGAGAACCAA 
GAGGTCATCG GTGTGGCTGA GCTAGTGAAC AAGATCAATG GGCCATGGTT CAGCAAATTT 
GATGAGGACC TGGCCACAGC CTTCTCCATC TACTGTGGCA TTAGCATCGC TCACTCTCTC 
CTATACAAAA AGGTGAATGA AGCCCAGTAC CGCAGCCACC TGGCCAACGA GATGATGATG 
TACCATATGA AGGTCTCTGA CGATGAATAC ACCAAACTTC TCCATGATGG CATCCAACCT 
GTGGCCGCCA TTGACTCCAA CTTTGCCAAC TTCACCTATA CTCCTCGGTC TCTGCCTGAG 
GACGATACTT CTATGGCCAT CCTGAGCATG CTGCAAGACA TGAACTTCAT CAATAACTAC 
AAAATTGACT GCCCAACTCT GGCCCGGTTC TGCCTGATGG TGAAGAAAGG CTACCGGGAT 
CCGCCCTACC ACAACTGGAT GCACGCCTTC TCTGTCTCTC ACTTTTGCTA CTTGCTCTAC 
AAGAATCTGG AGCTCTCCAA CTACCTCGAG GACATCGAGA TCTTTGCGTT GTTTATTTCC 
TGCATGTGTC ACGACCTGGA CCACAGAGGC ACAAACAACT CCTTCCAGGT GGCCTCGAAA 
TCTGTGCTGG CTGCACTCTA CAGCTCAGAG GGCTCTGTCA TGGAGAGGCA CCACTTTGCT 
CAGGCCATTG CTATCCTCAA CACCCATGGC TGTAATATCT TTGACCACTT CTCTCGGAAG 
GACTATCAGC GAATGCTGGA CCTGATGAGG GACATCATCT TGGCCACGGA CCTGGCACAC 
CACCTCCGCA TCTTCAAGGA CCTGCAGAAG ATGGCTGAAG TGGGTTATGA CCGAAACAAC 
AAGCAACACC ACAGGCTTCT TCTATGCCTC CTTATGACCT CCTGTGACCT CTCTGACCAG 
ACAAAGGGCT GGAAGACCAC CAGAAAGATT GCGGAGCTGA TCTACAAAGA GTTCTTCTCC 
CAAGGAGACT TGGAGAAGGC CATGGGCAAC CGACCAATGG AGATGATGGA TCGGGAGAAG 
GCCTACATCC CTGAACTTCA GATCAGCTTT ATGGAGCACA TCGCCATGCC CATCTACAAG 
CTGTTACAAG ACCTGTTCCC CAAGGCAGCA GAGCTGTATG AACGCGTGGC CTCCAATCGT 
GAGCACTGGA CCAAGGTGTC CCACAAGTTC ACCATCCGAG GCCTCCCCAG TAACAACTCG 
CTGGATTTCC TGGATGAGGA ATATGAGGTC CCTGATTTGG ACGTCACCAG GGCTCCTGTC 
AATGGCTGCT GCAGCCTCGA GGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pde2a ( NM_001270604.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001257533.1 Rattus norvegicus phosphodiesterase 2A (Pde2a), transcript variant 3, mRNA.

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CloneID	ORa09386
Clone ID Related Accession (Same CDS sequence)	NM_001270604.2 , NM_001270604.1
Accession Version	NM_001270604.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2751bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1532620800000
Organism	Rattus norvegicus(Norway rat)
Product	cGMP-dependent 3',5'-cyclic phosphodiesterase isoform PDE2A1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC128828.6 and AC122631.5. On Jan 18, 2018 this sequence version replaced NM_001270604.1. Transcript Variant: This variant (3) differs in the 5' UTR and has an alternate exon in the 5' UTR, compared to variant 1. These differences cause translation initiation at an alternate AUG and result in an isoform (PDE2A1) that is shorter and has a distinct N-terminus, compared to isoform PDE2A3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: FQ222940.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGCCGAC AGCCTGCAGC CAGCCAGGAC CCCCTTGCAC AGAAGCCAGA GCCCCCAGGG 
AGTAGAGACG ACCGACTGGA GGATGCTTTG CTGAGCCTGG GCGCCGTTAT CGACATCGCG 
GGCCTTCGAC AGGCTGCCAA GGATGCCCTC TCGGCCGTGC TCCCCAAAGT GGAGACTGTC 
TACACCTACC TGGTAGATGG GGAGTCCAGG CTGGTGTGTG AGGACCCCCC TCATGAGCTG 
CCACAGGAAG GAAAAATTCG AGAGGCTGTG ATTTCTCGGA AGCGGCTGAG CTGCGATGGG 
CTGGGACCTT CAGACCTACT AGGAAAGCCC TTGGCCAGGC TGGTGGCTCC ACTGGCCCCT 
GACACGCAAG TGCTGGTCAT ACCCCTGCTG GACAAGGAGA CTGGAACTGT GGCAGCCGTC 
ATCTTGGTGC ACTGTGGCCA GCTGAGTGAC AGTGAGGAGC AGAGCCTGCA GGTGGTGGAG 
AAGCATGCTC TGGTGGCCCT GCAGAGGGTG CAGGCCCTGC AGCAGCGCAG GCCTGAAGCT 
GTTCAGAACA CCTCAGCGGA TCCCTCTGAA GATCAAAAGG ATGAGAAGGG ATACACCGCC 
CATGACCGAA AGATCCTGCA ACTGTGTGGA GAACTCTATG ACTTGGATGC CACTTCTCTG 
CAGCTCAAAG TCCTTCGATA TCTTCAGCAG GAGACACAGG CCACTCACTG CTGCCTCCTG 
CTGGTGTCAG AGGACAACCT GCAGCTTTCC TGCAAGGTCA TTGGAGAGAA AGTGCTGGGA 
GAAGAGGTCA GCTTTCCATT GACCATGGGA CGCCTGGGCC AGGTGGTGGA GGACAAACAG 
TGTATCCAGT TGAAGGACCT AACCTCTGAC GATGTGCAAC AGCTACAAAA CATGTTGGGC 
TGTGAGCTTC GGGCTATGCT ATGTGTCCCT GTCATCAGTC GAGCCACTGA CCAGGTGGTG 
GCCCTGGCTT GCGCCTTCAA CAAGCTTGGA GGAGACTTCT TCACAGATGA GGATGAACGT 
GCGATCCAAC ACTGCTTCCA CTACACAGGC ACGGTGCTCA CCAGTACCTT GGCCTTCCAG 
AAGGAGCAGA AGCTCAAGTG TGAGTGCCAG GCTCTTCTCC AAGTGGCAAA GAACCTCTTC 
ACCCACCTGG ATGACGTCTC TGTCCTGCTA CAGGAGATCA TCACAGAGGC CAGAAACCTC 
AGCAACGCAG AGATCTGCTC CGTGTTCCTG CTGGATCAGA ATGAGCTGGT TGCCAAGGTG 
TTCGATGGCG GAGTAGTGGA CGATGAGAGT TATGAGATCC GCATCCCTGC GGACCAAGGC 
ATCGCGGGCC ACGTGGCGAC CACGGGCCAG ATCCTGAACA TCCCAGATGC ATACGCCCAT 
CCGCTTTTCT ATCGCGGCGT AGACGACAGC ACTGGCTTCC GCACGCGCAA CATTCTCTGC 
TTCCCTATCA AGAACGAGAA CCAAGAGGTC ATCGGTGTGG CTGAGCTAGT GAACAAGATC 
AATGGGCCAT GGTTCAGCAA ATTTGATGAG GACCTGGCCA CAGCCTTCTC CATCTACTGT 
GGCATTAGCA TCGCTCACTC TCTCCTATAC AAAAAGGTGA ATGAAGCCCA GTACCGCAGC 
CACCTGGCCA ACGAGATGAT GATGTACCAT ATGAAGGTCT CTGACGATGA ATACACCAAA 
CTTCTCCATG ATGGCATCCA ACCTGTGGCC GCCATTGACT CCAACTTTGC CAACTTCACC 
TATACTCCTC GGTCTCTGCC TGAGGACGAT ACTTCTATGG CCATCCTGAG CATGCTGCAA 
GACATGAACT TCATCAATAA CTACAAAATT GACTGCCCAA CTCTGGCCCG GTTCTGCCTG 
ATGGTGAAGA AAGGCTACCG GGATCCGCCC TACCACAACT GGATGCACGC CTTCTCTGTC 
TCTCACTTTT GCTACTTGCT CTACAAGAAT CTGGAGCTCT CCAACTACCT CGAGGACATC 
GAGATCTTTG CGTTGTTTAT TTCCTGCATG TGTCACGACC TGGACCACAG AGGCACAAAC 
AACTCCTTCC AGGTGGCCTC GAAATCTGTG CTGGCTGCAC TCTACAGCTC AGAGGGCTCT 
GTCATGGAGA GGCACCACTT TGCTCAGGCC ATTGCTATCC TCAACACCCA TGGCTGTAAT 
ATCTTTGACC ACTTCTCTCG GAAGGACTAT CAGCGAATGC TGGACCTGAT GAGGGACATC 
ATCTTGGCCA CGGACCTGGC ACACCACCTC CGCATCTTCA AGGACCTGCA GAAGATGGCT 
GAAGTGGGTT ATGACCGAAA CAACAAGCAA CACCACAGGC TTCTTCTATG CCTCCTTATG 
ACCTCCTGTG ACCTCTCTGA CCAGACAAAG GGCTGGAAGA CCACCAGAAA GATTGCGGAG 
CTGATCTACA AAGAGTTCTT CTCCCAAGGA GACTTGGAGA AGGCCATGGG CAACCGACCA 
ATGGAGATGA TGGATCGGGA GAAGGCCTAC ATCCCTGAAC TTCAGATCAG CTTTATGGAG 
CACATCGCCA TGCCCATCTA CAAGCTGTTA CAAGACCTGT TCCCCAAGGC AGCAGAGCTG 
TATGAACGCG TGGCCTCCAA TCGTGAGCAC TGGACCAAGG TGTCCCACAA GTTCACCATC 
CGAGGCCTCC CCAGTAACAA CTCGCTGGAT TTCCTGGATG AGGAATATGA GGTCCCTGAT 
TTGGACGTCA CCAGGGCTCC TGTCAATGGC TGCTGCAGCC TCGAGGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001257533.1
CDS	168..2918
Translation	MRRQPAASQDPLAQKPEPPGSRDDRLEDALLSLGAVIDIAGLRQAAKDALSAVLPKVETVYTYLVDGESRLVCEDPPHELPQEGKIREAVISRKRLSCDGLGPSDLLGKPLARLVAPLAPDTQVLVIPLLDKETGTVAAVILVHCGQLSDSEEQSLQVVEKHALVALQRVQALQQRRPEAVQNTSADPSEDQKDEKGYTAHDRKILQLCGELYDLDATSLQLKVLRYLQQETQATHCCLLLVSEDNLQLSCKVIGEKVLGEEVSFPLTMGRLGQVVEDKQCIQLKDLTSDDVQQLQNMLGCELRAMLCVPVISRATDQVVALACAFNKLGGDFFTDEDERAIQHCFHYTGTVLTSTLAFQKEQKLKCECQALLQVAKNLFTHLDDVSVLLQEIITEARNLSNAEICSVFLLDQNELVAKVFDGGVVDDESYEIRIPADQGIAGHVATTGQILNIPDAYAHPLFYRGVDDSTGFRTRNILCFPIKNENQEVIGVAELVNKINGPWFSKFDEDLATAFSIYCGISIAHSLLYKKVNEAQYRSHLANEMMMYHMKVSDDEYTKLLHDGIQPVAAIDSNFANFTYTPRSLPEDDTSMAILSMLQDMNFINNYKIDCPTLARFCLMVKKGYRDPPYHNWMHAFSVSHFCYLLYKNLELSNYLEDIEIFALFISCMCHDLDHRGTNNSFQVASKSVLAALYSSEGSVMERHHFAQAIAILNTHGCNIFDHFSRKDYQRMLDLMRDIILATDLAHHLRIFKDLQKMAEVGYDRNNKQHHRLLLCLLMTSCDLSDQTKGWKTTRKIAELIYKEFFSQGDLEKAMGNRPMEMMDREKAYIPELQISFMEHIAMPIYKLLQDLFPKAAELYERVASNREHWTKVSHKFTIRGLPSNNSLDFLDEEYEVPDLDVTRAPVNGCCSLEG

Target ORF information:

RefSeq Version	NM_001270604.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosphodiesterase 2A (Pde2a), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001270604.2

ORF Insert Sequence:

ATGCGCCGAC AGCCTGCAGC CAGCCAGGAC CCCCTTGCAC AGAAGCCAGA GCCCCCAGGG 
AGTAGAGACG ACCGACTGGA GGATGCTTTG CTGAGCCTGG GCGCCGTTAT CGACATCGCG 
GGCCTTCGAC AGGCTGCCAA GGATGCCCTC TCGGCCGTGC TCCCCAAAGT GGAGACTGTC 
TACACCTACC TGGTAGATGG GGAGTCCAGG CTGGTGTGTG AGGACCCCCC TCATGAGCTG 
CCACAGGAAG GAAAAATTCG AGAGGCTGTG ATTTCTCGGA AGCGGCTGAG CTGCGATGGG 
CTGGGACCTT CAGACCTACT AGGAAAGCCC TTGGCCAGGC TGGTGGCTCC ACTGGCCCCT 
GACACGCAAG TGCTGGTCAT ACCCCTGCTG GACAAGGAGA CTGGAACTGT GGCAGCCGTC 
ATCTTGGTGC ACTGTGGCCA GCTGAGTGAC AGTGAGGAGC AGAGCCTGCA GGTGGTGGAG 
AAGCATGCTC TGGTGGCCCT GCAGAGGGTG CAGGCCCTGC AGCAGCGCAG GCCTGAAGCT 
GTTCAGAACA CCTCAGCGGA TCCCTCTGAA GATCAAAAGG ATGAGAAGGG ATACACCGCC 
CATGACCGAA AGATCCTGCA ACTGTGTGGA GAACTCTATG ACTTGGATGC CACTTCTCTG 
CAGCTCAAAG TCCTTCGATA TCTTCAGCAG GAGACACAGG CCACTCACTG CTGCCTCCTG 
CTGGTGTCAG AGGACAACCT GCAGCTTTCC TGCAAGGTCA TTGGAGAGAA AGTGCTGGGA 
GAAGAGGTCA GCTTTCCATT GACCATGGGA CGCCTGGGCC AGGTGGTGGA GGACAAACAG 
TGTATCCAGT TGAAGGACCT AACCTCTGAC GATGTGCAAC AGCTACAAAA CATGTTGGGC 
TGTGAGCTTC GGGCTATGCT ATGTGTCCCT GTCATCAGTC GAGCCACTGA CCAGGTGGTG 
GCCCTGGCTT GCGCCTTCAA CAAGCTTGGA GGAGACTTCT TCACAGATGA GGATGAACGT 
GCGATCCAAC ACTGCTTCCA CTACACAGGC ACGGTGCTCA CCAGTACCTT GGCCTTCCAG 
AAGGAGCAGA AGCTCAAGTG TGAGTGCCAG GCTCTTCTCC AAGTGGCAAA GAACCTCTTC 
ACCCACCTGG ATGACGTCTC TGTCCTGCTA CAGGAGATCA TCACAGAGGC CAGAAACCTC 
AGCAACGCAG AGATCTGCTC CGTGTTCCTG CTGGATCAGA ATGAGCTGGT TGCCAAGGTG 
TTCGATGGCG GAGTAGTGGA CGATGAGAGT TATGAGATCC GCATCCCTGC GGACCAAGGC 
ATCGCGGGCC ACGTGGCGAC CACGGGCCAG ATCCTGAACA TCCCAGATGC ATACGCCCAT 
CCGCTTTTCT ATCGCGGCGT AGACGACAGC ACTGGCTTCC GCACGCGCAA CATTCTCTGC 
TTCCCTATCA AGAACGAGAA CCAAGAGGTC ATCGGTGTGG CTGAGCTAGT GAACAAGATC 
AATGGGCCAT GGTTCAGCAA ATTTGATGAG GACCTGGCCA CAGCCTTCTC CATCTACTGT 
GGCATTAGCA TCGCTCACTC TCTCCTATAC AAAAAGGTGA ATGAAGCCCA GTACCGCAGC 
CACCTGGCCA ACGAGATGAT GATGTACCAT ATGAAGGTCT CTGACGATGA ATACACCAAA 
CTTCTCCATG ATGGCATCCA ACCTGTGGCC GCCATTGACT CCAACTTTGC CAACTTCACC 
TATACTCCTC GGTCTCTGCC TGAGGACGAT ACTTCTATGG CCATCCTGAG CATGCTGCAA 
GACATGAACT TCATCAATAA CTACAAAATT GACTGCCCAA CTCTGGCCCG GTTCTGCCTG 
ATGGTGAAGA AAGGCTACCG GGATCCGCCC TACCACAACT GGATGCACGC CTTCTCTGTC 
TCTCACTTTT GCTACTTGCT CTACAAGAAT CTGGAGCTCT CCAACTACCT CGAGGACATC 
GAGATCTTTG CGTTGTTTAT TTCCTGCATG TGTCACGACC TGGACCACAG AGGCACAAAC 
AACTCCTTCC AGGTGGCCTC GAAATCTGTG CTGGCTGCAC TCTACAGCTC AGAGGGCTCT 
GTCATGGAGA GGCACCACTT TGCTCAGGCC ATTGCTATCC TCAACACCCA TGGCTGTAAT 
ATCTTTGACC ACTTCTCTCG GAAGGACTAT CAGCGAATGC TGGACCTGAT GAGGGACATC 
ATCTTGGCCA CGGACCTGGC ACACCACCTC CGCATCTTCA AGGACCTGCA GAAGATGGCT 
GAAGTGGGTT ATGACCGAAA CAACAAGCAA CACCACAGGC TTCTTCTATG CCTCCTTATG 
ACCTCCTGTG ACCTCTCTGA CCAGACAAAG GGCTGGAAGA CCACCAGAAA GATTGCGGAG 
CTGATCTACA AAGAGTTCTT CTCCCAAGGA GACTTGGAGA AGGCCATGGG CAACCGACCA 
ATGGAGATGA TGGATCGGGA GAAGGCCTAC ATCCCTGAAC TTCAGATCAG CTTTATGGAG 
CACATCGCCA TGCCCATCTA CAAGCTGTTA CAAGACCTGT TCCCCAAGGC AGCAGAGCTG 
TATGAACGCG TGGCCTCCAA TCGTGAGCAC TGGACCAAGG TGTCCCACAA GTTCACCATC 
CGAGGCCTCC CCAGTAACAA CTCGCTGGAT TTCCTGGATG AGGAATATGA GGTCCCTGAT 
TTGGACGTCA CCAGGGCTCC TGTCAATGGC TGCTGCAGCC TCGAGGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pde2a ( NM_001270604.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001257533.1 Rattus norvegicus phosphodiesterase 2A (Pde2a), transcript variant 3, mRNA.

Price & Availability↑

CloneID	ORa09386
Clone ID Related Accession (Same CDS sequence)	NM_001270604.2 , NM_001270604.1
Accession Version	NM_001270604.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2751bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1506873600000
Organism	Rattus norvegicus(Norway rat)
Product	cGMP-dependent 3',5'-cyclic phosphodiesterase isoform PDE2A1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC128828.6 and AC122631.5. Transcript Variant: This variant (2) differs in the 5' UTR and has an alternate exon in the 5' UTR, compared to variant 1. These differences cause translation initiation at an alternate AUG and result in an isoform (PDE2A1) that is shorter and has a distinct N-terminus, compared to isoform PDE2A3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: FQ222940.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGCGCCGAC AGCCTGCAGC CAGCCAGGAC CCCCTTGCAC AGAAGCCAGA GCCCCCAGGG 
AGTAGAGACG ACCGACTGGA GGATGCTTTG CTGAGCCTGG GCGCCGTTAT CGACATCGCG 
GGCCTTCGAC AGGCTGCCAA GGATGCCCTC TCGGCCGTGC TCCCCAAAGT GGAGACTGTC 
TACACCTACC TGGTAGATGG GGAGTCCAGG CTGGTGTGTG AGGACCCCCC TCATGAGCTG 
CCACAGGAAG GAAAAATTCG AGAGGCTGTG ATTTCTCGGA AGCGGCTGAG CTGCGATGGG 
CTGGGACCTT CAGACCTACT AGGAAAGCCC TTGGCCAGGC TGGTGGCTCC ACTGGCCCCT 
GACACGCAAG TGCTGGTCAT ACCCCTGCTG GACAAGGAGA CTGGAACTGT GGCAGCCGTC 
ATCTTGGTGC ACTGTGGCCA GCTGAGTGAC AGTGAGGAGC AGAGCCTGCA GGTGGTGGAG 
AAGCATGCTC TGGTGGCCCT GCAGAGGGTG CAGGCCCTGC AGCAGCGCAG GCCTGAAGCT 
GTTCAGAACA CCTCAGCGGA TCCCTCTGAA GATCAAAAGG ATGAGAAGGG ATACACCGCC 
CATGACCGAA AGATCCTGCA ACTGTGTGGA GAACTCTATG ACTTGGATGC CACTTCTCTG 
CAGCTCAAAG TCCTTCGATA TCTTCAGCAG GAGACACAGG CCACTCACTG CTGCCTCCTG 
CTGGTGTCAG AGGACAACCT GCAGCTTTCC TGCAAGGTCA TTGGAGAGAA AGTGCTGGGA 
GAAGAGGTCA GCTTTCCATT GACCATGGGA CGCCTGGGCC AGGTGGTGGA GGACAAACAG 
TGTATCCAGT TGAAGGACCT AACCTCTGAC GATGTGCAAC AGCTACAAAA CATGTTGGGC 
TGTGAGCTTC GGGCTATGCT ATGTGTCCCT GTCATCAGTC GAGCCACTGA CCAGGTGGTG 
GCCCTGGCTT GCGCCTTCAA CAAGCTTGGA GGAGACTTCT TCACAGATGA GGATGAACGT 
GCGATCCAAC ACTGCTTCCA CTACACAGGC ACGGTGCTCA CCAGTACCTT GGCCTTCCAG 
AAGGAGCAGA AGCTCAAGTG TGAGTGCCAG GCTCTTCTCC AAGTGGCAAA GAACCTCTTC 
ACCCACCTGG ATGACGTCTC TGTCCTGCTA CAGGAGATCA TCACAGAGGC CAGAAACCTC 
AGCAACGCAG AGATCTGCTC CGTGTTCCTG CTGGATCAGA ATGAGCTGGT TGCCAAGGTG 
TTCGATGGCG GAGTAGTGGA CGATGAGAGT TATGAGATCC GCATCCCTGC GGACCAAGGC 
ATCGCGGGCC ACGTGGCGAC CACGGGCCAG ATCCTGAACA TCCCAGATGC ATACGCCCAT 
CCGCTTTTCT ATCGCGGCGT AGACGACAGC ACTGGCTTCC GCACGCGCAA CATTCTCTGC 
TTCCCTATCA AGAACGAGAA CCAAGAGGTC ATCGGTGTGG CTGAGCTAGT GAACAAGATC 
AATGGGCCAT GGTTCAGCAA ATTTGATGAG GACCTGGCCA CAGCCTTCTC CATCTACTGT 
GGCATTAGCA TCGCTCACTC TCTCCTATAC AAAAAGGTGA ATGAAGCCCA GTACCGCAGC 
CACCTGGCCA ACGAGATGAT GATGTACCAT ATGAAGGTCT CTGACGATGA ATACACCAAA 
CTTCTCCATG ATGGCATCCA ACCTGTGGCC GCCATTGACT CCAACTTTGC CAACTTCACC 
TATACTCCTC GGTCTCTGCC TGAGGACGAT ACTTCTATGG CCATCCTGAG CATGCTGCAA 
GACATGAACT TCATCAATAA CTACAAAATT GACTGCCCAA CTCTGGCCCG GTTCTGCCTG 
ATGGTGAAGA AAGGCTACCG GGATCCGCCC TACCACAACT GGATGCACGC CTTCTCTGTC 
TCTCACTTTT GCTACTTGCT CTACAAGAAT CTGGAGCTCT CCAACTACCT CGAGGACATC 
GAGATCTTTG CGTTGTTTAT TTCCTGCATG TGTCACGACC TGGACCACAG AGGCACAAAC 
AACTCCTTCC AGGTGGCCTC GAAATCTGTG CTGGCTGCAC TCTACAGCTC AGAGGGCTCT 
GTCATGGAGA GGCACCACTT TGCTCAGGCC ATTGCTATCC TCAACACCCA TGGCTGTAAT 
ATCTTTGACC ACTTCTCTCG GAAGGACTAT CAGCGAATGC TGGACCTGAT GAGGGACATC 
ATCTTGGCCA CGGACCTGGC ACACCACCTC CGCATCTTCA AGGACCTGCA GAAGATGGCT 
GAAGTGGGTT ATGACCGAAA CAACAAGCAA CACCACAGGC TTCTTCTATG CCTCCTTATG 
ACCTCCTGTG ACCTCTCTGA CCAGACAAAG GGCTGGAAGA CCACCAGAAA GATTGCGGAG 
CTGATCTACA AAGAGTTCTT CTCCCAAGGA GACTTGGAGA AGGCCATGGG CAACCGACCA 
ATGGAGATGA TGGATCGGGA GAAGGCCTAC ATCCCTGAAC TTCAGATCAG CTTTATGGAG 
CACATCGCCA TGCCCATCTA CAAGCTGTTA CAAGACCTGT TCCCCAAGGC AGCAGAGCTG 
TATGAACGCG TGGCCTCCAA TCGTGAGCAC TGGACCAAGG TGTCCCACAA GTTCACCATC 
CGAGGCCTCC CCAGTAACAA CTCGCTGGAT TTCCTGGATG AGGAATATGA GGTCCCTGAT 
TTGGACGTCA CCAGGGCTCC TGTCAATGGC TGCTGCAGCC TCGAGGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001257533.1
CDS	199..2949
Translation	MRRQPAASQDPLAQKPEPPGSRDDRLEDALLSLGAVIDIAGLRQAAKDALSAVLPKVETVYTYLVDGESRLVCEDPPHELPQEGKIREAVISRKRLSCDGLGPSDLLGKPLARLVAPLAPDTQVLVIPLLDKETGTVAAVILVHCGQLSDSEEQSLQVVEKHALVALQRVQALQQRRPEAVQNTSADPSEDQKDEKGYTAHDRKILQLCGELYDLDATSLQLKVLRYLQQETQATHCCLLLVSEDNLQLSCKVIGEKVLGEEVSFPLTMGRLGQVVEDKQCIQLKDLTSDDVQQLQNMLGCELRAMLCVPVISRATDQVVALACAFNKLGGDFFTDEDERAIQHCFHYTGTVLTSTLAFQKEQKLKCECQALLQVAKNLFTHLDDVSVLLQEIITEARNLSNAEICSVFLLDQNELVAKVFDGGVVDDESYEIRIPADQGIAGHVATTGQILNIPDAYAHPLFYRGVDDSTGFRTRNILCFPIKNENQEVIGVAELVNKINGPWFSKFDEDLATAFSIYCGISIAHSLLYKKVNEAQYRSHLANEMMMYHMKVSDDEYTKLLHDGIQPVAAIDSNFANFTYTPRSLPEDDTSMAILSMLQDMNFINNYKIDCPTLARFCLMVKKGYRDPPYHNWMHAFSVSHFCYLLYKNLELSNYLEDIEIFALFISCMCHDLDHRGTNNSFQVASKSVLAALYSSEGSVMERHHFAQAIAILNTHGCNIFDHFSRKDYQRMLDLMRDIILATDLAHHLRIFKDLQKMAEVGYDRNNKQHHRLLLCLLMTSCDLSDQTKGWKTTRKIAELIYKEFFSQGDLEKAMGNRPMEMMDREKAYIPELQISFMEHIAMPIYKLLQDLFPKAAELYERVASNREHWTKVSHKFTIRGLPSNNSLDFLDEEYEVPDLDVTRAPVNGCCSLEG

Target ORF information:

RefSeq Version	NM_001270604.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosphodiesterase 2A (Pde2a), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001270604.1

ORF Insert Sequence:

ATGCGCCGAC AGCCTGCAGC CAGCCAGGAC CCCCTTGCAC AGAAGCCAGA GCCCCCAGGG 
AGTAGAGACG ACCGACTGGA GGATGCTTTG CTGAGCCTGG GCGCCGTTAT CGACATCGCG 
GGCCTTCGAC AGGCTGCCAA GGATGCCCTC TCGGCCGTGC TCCCCAAAGT GGAGACTGTC 
TACACCTACC TGGTAGATGG GGAGTCCAGG CTGGTGTGTG AGGACCCCCC TCATGAGCTG 
CCACAGGAAG GAAAAATTCG AGAGGCTGTG ATTTCTCGGA AGCGGCTGAG CTGCGATGGG 
CTGGGACCTT CAGACCTACT AGGAAAGCCC TTGGCCAGGC TGGTGGCTCC ACTGGCCCCT 
GACACGCAAG TGCTGGTCAT ACCCCTGCTG GACAAGGAGA CTGGAACTGT GGCAGCCGTC 
ATCTTGGTGC ACTGTGGCCA GCTGAGTGAC AGTGAGGAGC AGAGCCTGCA GGTGGTGGAG 
AAGCATGCTC TGGTGGCCCT GCAGAGGGTG CAGGCCCTGC AGCAGCGCAG GCCTGAAGCT 
GTTCAGAACA CCTCAGCGGA TCCCTCTGAA GATCAAAAGG ATGAGAAGGG ATACACCGCC 
CATGACCGAA AGATCCTGCA ACTGTGTGGA GAACTCTATG ACTTGGATGC CACTTCTCTG 
CAGCTCAAAG TCCTTCGATA TCTTCAGCAG GAGACACAGG CCACTCACTG CTGCCTCCTG 
CTGGTGTCAG AGGACAACCT GCAGCTTTCC TGCAAGGTCA TTGGAGAGAA AGTGCTGGGA 
GAAGAGGTCA GCTTTCCATT GACCATGGGA CGCCTGGGCC AGGTGGTGGA GGACAAACAG 
TGTATCCAGT TGAAGGACCT AACCTCTGAC GATGTGCAAC AGCTACAAAA CATGTTGGGC 
TGTGAGCTTC GGGCTATGCT ATGTGTCCCT GTCATCAGTC GAGCCACTGA CCAGGTGGTG 
GCCCTGGCTT GCGCCTTCAA CAAGCTTGGA GGAGACTTCT TCACAGATGA GGATGAACGT 
GCGATCCAAC ACTGCTTCCA CTACACAGGC ACGGTGCTCA CCAGTACCTT GGCCTTCCAG 
AAGGAGCAGA AGCTCAAGTG TGAGTGCCAG GCTCTTCTCC AAGTGGCAAA GAACCTCTTC 
ACCCACCTGG ATGACGTCTC TGTCCTGCTA CAGGAGATCA TCACAGAGGC CAGAAACCTC 
AGCAACGCAG AGATCTGCTC CGTGTTCCTG CTGGATCAGA ATGAGCTGGT TGCCAAGGTG 
TTCGATGGCG GAGTAGTGGA CGATGAGAGT TATGAGATCC GCATCCCTGC GGACCAAGGC 
ATCGCGGGCC ACGTGGCGAC CACGGGCCAG ATCCTGAACA TCCCAGATGC ATACGCCCAT 
CCGCTTTTCT ATCGCGGCGT AGACGACAGC ACTGGCTTCC GCACGCGCAA CATTCTCTGC 
TTCCCTATCA AGAACGAGAA CCAAGAGGTC ATCGGTGTGG CTGAGCTAGT GAACAAGATC 
AATGGGCCAT GGTTCAGCAA ATTTGATGAG GACCTGGCCA CAGCCTTCTC CATCTACTGT 
GGCATTAGCA TCGCTCACTC TCTCCTATAC AAAAAGGTGA ATGAAGCCCA GTACCGCAGC 
CACCTGGCCA ACGAGATGAT GATGTACCAT ATGAAGGTCT CTGACGATGA ATACACCAAA 
CTTCTCCATG ATGGCATCCA ACCTGTGGCC GCCATTGACT CCAACTTTGC CAACTTCACC 
TATACTCCTC GGTCTCTGCC TGAGGACGAT ACTTCTATGG CCATCCTGAG CATGCTGCAA 
GACATGAACT TCATCAATAA CTACAAAATT GACTGCCCAA CTCTGGCCCG GTTCTGCCTG 
ATGGTGAAGA AAGGCTACCG GGATCCGCCC TACCACAACT GGATGCACGC CTTCTCTGTC 
TCTCACTTTT GCTACTTGCT CTACAAGAAT CTGGAGCTCT CCAACTACCT CGAGGACATC 
GAGATCTTTG CGTTGTTTAT TTCCTGCATG TGTCACGACC TGGACCACAG AGGCACAAAC 
AACTCCTTCC AGGTGGCCTC GAAATCTGTG CTGGCTGCAC TCTACAGCTC AGAGGGCTCT 
GTCATGGAGA GGCACCACTT TGCTCAGGCC ATTGCTATCC TCAACACCCA TGGCTGTAAT 
ATCTTTGACC ACTTCTCTCG GAAGGACTAT CAGCGAATGC TGGACCTGAT GAGGGACATC 
ATCTTGGCCA CGGACCTGGC ACACCACCTC CGCATCTTCA AGGACCTGCA GAAGATGGCT 
GAAGTGGGTT ATGACCGAAA CAACAAGCAA CACCACAGGC TTCTTCTATG CCTCCTTATG 
ACCTCCTGTG ACCTCTCTGA CCAGACAAAG GGCTGGAAGA CCACCAGAAA GATTGCGGAG 
CTGATCTACA AAGAGTTCTT CTCCCAAGGA GACTTGGAGA AGGCCATGGG CAACCGACCA 
ATGGAGATGA TGGATCGGGA GAAGGCCTAC ATCCCTGAAC TTCAGATCAG CTTTATGGAG 
CACATCGCCA TGCCCATCTA CAAGCTGTTA CAAGACCTGT TCCCCAAGGC AGCAGAGCTG 
TATGAACGCG TGGCCTCCAA TCGTGAGCAC TGGACCAAGG TGTCCCACAA GTTCACCATC 
CGAGGCCTCC CCAGTAACAA CTCGCTGGAT TTCCTGGATG AGGAATATGA GGTCCCTGAT 
TTGGACGTCA CCAGGGCTCC TGTCAATGGC TGCTGCAGCC TCGAGGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pde2a ( NM_001143847.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001137319.1 Rattus norvegicus phosphodiesterase 2A (Pde2a), transcript variant 1, mRNA.

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CloneID	ORa63305
Clone ID Related Accession (Same CDS sequence)	NM_001143847.2
Accession Version	NM_001143847.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2808bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1532620800000
Organism	Rattus norvegicus(Norway rat)
Product	cGMP-dependent 3',5'-cyclic phosphodiesterase isoform PDE2A3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC128828.6 and AC122631.5. On Jan 18, 2018 this sequence version replaced NM_001143847.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (PDE2A3). This variant is assembled from partial rat transcripts. The full-length exon combination is based on the mouse ortholog, NM_001008548.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGCAGG CATGCGGCCA CTCCATCCTC TGCAGGAGCC AGCAGTACCC GGCAGCGCGA 
CCGGCTGAGC CGCGGGGCCA GCAGGTCTTC CTCAAGCCGG ACGAACCGCC GCCGCAGCCA 
TGCGCCGACA GCCTGCAGGA TGCTTTGCTG AGCCTGGGCG CCGTTATCGA CATCGCGGGC 
CTTCGACAGG CTGCCAAGGA TGCCCTCTCG GCCGTGCTCC CCAAAGTGGA GACTGTCTAC 
ACCTACCTGG TAGATGGGGA GTCCAGGCTG GTGTGTGAGG ACCCCCCTCA TGAGCTGCCA 
CAGGAAGGAA AAATTCGAGA GGCTGTGATT TCTCGGAAGC GGCTGAGCTG CGATGGGCTG 
GGACCTTCAG ACCTACTAGG AAAGCCCTTG GCCAGGCTGG TGGCTCCACT GGCCCCTGAC 
ACGCAAGTGC TGGTCATACC CCTGCTGGAC AAGGAGACTG GAACTGTGGC AGCCGTCATC 
TTGGTGCACT GTGGCCAGCT GAGTGACAGT GAGGAGCAGA GCCTGCAGGT GGTGGAGAAG 
CATGCTCTGG TGGCCCTGCA GAGGGTGCAG GCCCTGCAGC AGCGCAGGCC TGAAGCTGTT 
CAGAACACCT CAGCGGATCC CTCTGAAGAT CAAAAGGATG AGAAGGGATA CACCGCCCAT 
GACCGAAAGA TCCTGCAACT GTGTGGAGAA CTCTATGACT TGGATGCCAC TTCTCTGCAG 
CTCAAAGTCC TTCGATATCT TCAGCAGGAG ACACAGGCCA CTCACTGCTG CCTCCTGCTG 
GTGTCAGAGG ACAACCTGCA GCTTTCCTGC AAGGTCATTG GAGAGAAAGT GCTGGGAGAA 
GAGGTCAGCT TTCCATTGAC CATGGGACGC CTGGGCCAGG TGGTGGAGGA CAAACAGTGT 
ATCCAGTTGA AGGACCTAAC CTCTGACGAT GTGCAACAGC TACAAAACAT GTTGGGCTGT 
GAGCTTCGGG CTATGCTATG TGTCCCTGTC ATCAGTCGAG CCACTGACCA GGTGGTGGCC 
CTGGCTTGCG CCTTCAACAA GCTTGGAGGA GACTTCTTCA CAGATGAGGA TGAACGTGCG 
ATCCAACACT GCTTCCACTA CACAGGCACG GTGCTCACCA GTACCTTGGC CTTCCAGAAG 
GAGCAGAAGC TCAAGTGTGA GTGCCAGGCT CTTCTCCAAG TGGCAAAGAA CCTCTTCACC 
CACCTGGATG ACGTCTCTGT CCTGCTACAG GAGATCATCA CAGAGGCCAG AAACCTCAGC 
AACGCAGAGA TCTGCTCCGT GTTCCTGCTG GATCAGAATG AGCTGGTTGC CAAGGTGTTC 
GATGGCGGAG TAGTGGACGA TGAGAGTTAT GAGATCCGCA TCCCTGCGGA CCAAGGCATC 
GCGGGCCACG TGGCGACCAC GGGCCAGATC CTGAACATCC CAGATGCATA CGCCCATCCG 
CTTTTCTATC GCGGCGTAGA CGACAGCACT GGCTTCCGCA CGCGCAACAT TCTCTGCTTC 
CCTATCAAGA ACGAGAACCA AGAGGTCATC GGTGTGGCTG AGCTAGTGAA CAAGATCAAT 
GGGCCATGGT TCAGCAAATT TGATGAGGAC CTGGCCACAG CCTTCTCCAT CTACTGTGGC 
ATTAGCATCG CTCACTCTCT CCTATACAAA AAGGTGAATG AAGCCCAGTA CCGCAGCCAC 
CTGGCCAACG AGATGATGAT GTACCATATG AAGGTCTCTG ACGATGAATA CACCAAACTT 
CTCCATGATG GCATCCAACC TGTGGCCGCC ATTGACTCCA ACTTTGCCAA CTTCACCTAT 
ACTCCTCGGT CTCTGCCTGA GGACGATACT TCTATGGCCA TCCTGAGCAT GCTGCAAGAC 
ATGAACTTCA TCAATAACTA CAAAATTGAC TGCCCAACTC TGGCCCGGTT CTGCCTGATG 
GTGAAGAAAG GCTACCGGGA TCCGCCCTAC CACAACTGGA TGCACGCCTT CTCTGTCTCT 
CACTTTTGCT ACTTGCTCTA CAAGAATCTG GAGCTCTCCA ACTACCTCGA GGACATCGAG 
ATCTTTGCGT TGTTTATTTC CTGCATGTGT CACGACCTGG ACCACAGAGG CACAAACAAC 
TCCTTCCAGG TGGCCTCGAA ATCTGTGCTG GCTGCACTCT ACAGCTCAGA GGGCTCTGTC 
ATGGAGAGGC ACCACTTTGC TCAGGCCATT GCTATCCTCA ACACCCATGG CTGTAATATC 
TTTGACCACT TCTCTCGGAA GGACTATCAG CGAATGCTGG ACCTGATGAG GGACATCATC 
TTGGCCACGG ACCTGGCACA CCACCTCCGC ATCTTCAAGG ACCTGCAGAA GATGGCTGAA 
GTGGGTTATG ACCGAAACAA CAAGCAACAC CACAGGCTTC TTCTATGCCT CCTTATGACC 
TCCTGTGACC TCTCTGACCA GACAAAGGGC TGGAAGACCA CCAGAAAGAT TGCGGAGCTG 
ATCTACAAAG AGTTCTTCTC CCAAGGAGAC TTGGAGAAGG CCATGGGCAA CCGACCAATG 
GAGATGATGG ATCGGGAGAA GGCCTACATC CCTGAACTTC AGATCAGCTT TATGGAGCAC 
ATCGCCATGC CCATCTACAA GCTGTTACAA GACCTGTTCC CCAAGGCAGC AGAGCTGTAT 
GAACGCGTGG CCTCCAATCG TGAGCACTGG ACCAAGGTGT CCCACAAGTT CACCATCCGA 
GGCCTCCCCA GTAACAACTC GCTGGATTTC CTGGATGAGG AATATGAGGT CCCTGATTTG 
GACGTCACCA GGGCTCCTGT CAATGGCTGC TGCAGCCTCG AGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137319.1
CDS	231..3038
Translation	MGQACGHSILCRSQQYPAARPAEPRGQQVFLKPDEPPPQPCADSLQDALLSLGAVIDIAGLRQAAKDALSAVLPKVETVYTYLVDGESRLVCEDPPHELPQEGKIREAVISRKRLSCDGLGPSDLLGKPLARLVAPLAPDTQVLVIPLLDKETGTVAAVILVHCGQLSDSEEQSLQVVEKHALVALQRVQALQQRRPEAVQNTSADPSEDQKDEKGYTAHDRKILQLCGELYDLDATSLQLKVLRYLQQETQATHCCLLLVSEDNLQLSCKVIGEKVLGEEVSFPLTMGRLGQVVEDKQCIQLKDLTSDDVQQLQNMLGCELRAMLCVPVISRATDQVVALACAFNKLGGDFFTDEDERAIQHCFHYTGTVLTSTLAFQKEQKLKCECQALLQVAKNLFTHLDDVSVLLQEIITEARNLSNAEICSVFLLDQNELVAKVFDGGVVDDESYEIRIPADQGIAGHVATTGQILNIPDAYAHPLFYRGVDDSTGFRTRNILCFPIKNENQEVIGVAELVNKINGPWFSKFDEDLATAFSIYCGISIAHSLLYKKVNEAQYRSHLANEMMMYHMKVSDDEYTKLLHDGIQPVAAIDSNFANFTYTPRSLPEDDTSMAILSMLQDMNFINNYKIDCPTLARFCLMVKKGYRDPPYHNWMHAFSVSHFCYLLYKNLELSNYLEDIEIFALFISCMCHDLDHRGTNNSFQVASKSVLAALYSSEGSVMERHHFAQAIAILNTHGCNIFDHFSRKDYQRMLDLMRDIILATDLAHHLRIFKDLQKMAEVGYDRNNKQHHRLLLCLLMTSCDLSDQTKGWKTTRKIAELIYKEFFSQGDLEKAMGNRPMEMMDREKAYIPELQISFMEHIAMPIYKLLQDLFPKAAELYERVASNREHWTKVSHKFTIRGLPSNNSLDFLDEEYEVPDLDVTRAPVNGCCSLEG

Target ORF information:

RefSeq Version	NM_001143847.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosphodiesterase 2A (Pde2a), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001143847.2

ORF Insert Sequence:

ATGGGGCAGG CATGCGGCCA CTCCATCCTC TGCAGGAGCC AGCAGTACCC GGCAGCGCGA 
CCGGCTGAGC CGCGGGGCCA GCAGGTCTTC CTCAAGCCGG ACGAACCGCC GCCGCAGCCA 
TGCGCCGACA GCCTGCAGGA TGCTTTGCTG AGCCTGGGCG CCGTTATCGA CATCGCGGGC 
CTTCGACAGG CTGCCAAGGA TGCCCTCTCG GCCGTGCTCC CCAAAGTGGA GACTGTCTAC 
ACCTACCTGG TAGATGGGGA GTCCAGGCTG GTGTGTGAGG ACCCCCCTCA TGAGCTGCCA 
CAGGAAGGAA AAATTCGAGA GGCTGTGATT TCTCGGAAGC GGCTGAGCTG CGATGGGCTG 
GGACCTTCAG ACCTACTAGG AAAGCCCTTG GCCAGGCTGG TGGCTCCACT GGCCCCTGAC 
ACGCAAGTGC TGGTCATACC CCTGCTGGAC AAGGAGACTG GAACTGTGGC AGCCGTCATC 
TTGGTGCACT GTGGCCAGCT GAGTGACAGT GAGGAGCAGA GCCTGCAGGT GGTGGAGAAG 
CATGCTCTGG TGGCCCTGCA GAGGGTGCAG GCCCTGCAGC AGCGCAGGCC TGAAGCTGTT 
CAGAACACCT CAGCGGATCC CTCTGAAGAT CAAAAGGATG AGAAGGGATA CACCGCCCAT 
GACCGAAAGA TCCTGCAACT GTGTGGAGAA CTCTATGACT TGGATGCCAC TTCTCTGCAG 
CTCAAAGTCC TTCGATATCT TCAGCAGGAG ACACAGGCCA CTCACTGCTG CCTCCTGCTG 
GTGTCAGAGG ACAACCTGCA GCTTTCCTGC AAGGTCATTG GAGAGAAAGT GCTGGGAGAA 
GAGGTCAGCT TTCCATTGAC CATGGGACGC CTGGGCCAGG TGGTGGAGGA CAAACAGTGT 
ATCCAGTTGA AGGACCTAAC CTCTGACGAT GTGCAACAGC TACAAAACAT GTTGGGCTGT 
GAGCTTCGGG CTATGCTATG TGTCCCTGTC ATCAGTCGAG CCACTGACCA GGTGGTGGCC 
CTGGCTTGCG CCTTCAACAA GCTTGGAGGA GACTTCTTCA CAGATGAGGA TGAACGTGCG 
ATCCAACACT GCTTCCACTA CACAGGCACG GTGCTCACCA GTACCTTGGC CTTCCAGAAG 
GAGCAGAAGC TCAAGTGTGA GTGCCAGGCT CTTCTCCAAG TGGCAAAGAA CCTCTTCACC 
CACCTGGATG ACGTCTCTGT CCTGCTACAG GAGATCATCA CAGAGGCCAG AAACCTCAGC 
AACGCAGAGA TCTGCTCCGT GTTCCTGCTG GATCAGAATG AGCTGGTTGC CAAGGTGTTC 
GATGGCGGAG TAGTGGACGA TGAGAGTTAT GAGATCCGCA TCCCTGCGGA CCAAGGCATC 
GCGGGCCACG TGGCGACCAC GGGCCAGATC CTGAACATCC CAGATGCATA CGCCCATCCG 
CTTTTCTATC GCGGCGTAGA CGACAGCACT GGCTTCCGCA CGCGCAACAT TCTCTGCTTC 
CCTATCAAGA ACGAGAACCA AGAGGTCATC GGTGTGGCTG AGCTAGTGAA CAAGATCAAT 
GGGCCATGGT TCAGCAAATT TGATGAGGAC CTGGCCACAG CCTTCTCCAT CTACTGTGGC 
ATTAGCATCG CTCACTCTCT CCTATACAAA AAGGTGAATG AAGCCCAGTA CCGCAGCCAC 
CTGGCCAACG AGATGATGAT GTACCATATG AAGGTCTCTG ACGATGAATA CACCAAACTT 
CTCCATGATG GCATCCAACC TGTGGCCGCC ATTGACTCCA ACTTTGCCAA CTTCACCTAT 
ACTCCTCGGT CTCTGCCTGA GGACGATACT TCTATGGCCA TCCTGAGCAT GCTGCAAGAC 
ATGAACTTCA TCAATAACTA CAAAATTGAC TGCCCAACTC TGGCCCGGTT CTGCCTGATG 
GTGAAGAAAG GCTACCGGGA TCCGCCCTAC CACAACTGGA TGCACGCCTT CTCTGTCTCT 
CACTTTTGCT ACTTGCTCTA CAAGAATCTG GAGCTCTCCA ACTACCTCGA GGACATCGAG 
ATCTTTGCGT TGTTTATTTC CTGCATGTGT CACGACCTGG ACCACAGAGG CACAAACAAC 
TCCTTCCAGG TGGCCTCGAA ATCTGTGCTG GCTGCACTCT ACAGCTCAGA GGGCTCTGTC 
ATGGAGAGGC ACCACTTTGC TCAGGCCATT GCTATCCTCA ACACCCATGG CTGTAATATC 
TTTGACCACT TCTCTCGGAA GGACTATCAG CGAATGCTGG ACCTGATGAG GGACATCATC 
TTGGCCACGG ACCTGGCACA CCACCTCCGC ATCTTCAAGG ACCTGCAGAA GATGGCTGAA 
GTGGGTTATG ACCGAAACAA CAAGCAACAC CACAGGCTTC TTCTATGCCT CCTTATGACC 
TCCTGTGACC TCTCTGACCA GACAAAGGGC TGGAAGACCA CCAGAAAGAT TGCGGAGCTG 
ATCTACAAAG AGTTCTTCTC CCAAGGAGAC TTGGAGAAGG CCATGGGCAA CCGACCAATG 
GAGATGATGG ATCGGGAGAA GGCCTACATC CCTGAACTTC AGATCAGCTT TATGGAGCAC 
ATCGCCATGC CCATCTACAA GCTGTTACAA GACCTGTTCC CCAAGGCAGC AGAGCTGTAT 
GAACGCGTGG CCTCCAATCG TGAGCACTGG ACCAAGGTGT CCCACAAGTT CACCATCCGA 
GGCCTCCCCA GTAACAACTC GCTGGATTTC CTGGATGAGG AATATGAGGT CCCTGATTTG 
GACGTCACCA GGGCTCCTGT CAATGGCTGC TGCAGCCTCG AGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pde2a ( NM_031079.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_112341.1 Rattus norvegicus phosphodiesterase 2A (Pde2a), transcript variant 2, mRNA.

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CloneID	ORa63312
Clone ID Related Accession (Same CDS sequence)	NM_031079.2
Accession Version	NM_031079.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2787bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1551110400000
Organism	Rattus norvegicus(Norway rat)
Product	cGMP-dependent 3',5'-cyclic phosphodiesterase isoform PDE2A2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC128828.6 and AC122631.5. On Jan 18, 2018 this sequence version replaced NM_031079.1. Transcript Variant: This variant (2) represents use of an alternate promoter and has a distinct 5' UTR and 5' coding region, compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U21101.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTCCTGG TGTTGCACCA CATCCTCATC GCTGTTGTCC AATTCCTCAG GCGGGGCCAG 
CAGGTCTTCC TCAAGCCGGA CGAACCGCCG CCGCAGCCAT GCGCCGACAG CCTGCAGGAT 
GCTTTGCTGA GCCTGGGCGC CGTTATCGAC ATCGCGGGCC TTCGACAGGC TGCCAAGGAT 
GCCCTCTCGG CCGTGCTCCC CAAAGTGGAG ACTGTCTACA CCTACCTGGT AGATGGGGAG 
TCCAGGCTGG TGTGTGAGGA CCCCCCTCAT GAGCTGCCAC AGGAAGGAAA AATTCGAGAG 
GCTGTGATTT CTCGGAAGCG GCTGAGCTGC GATGGGCTGG GACCTTCAGA CCTACTAGGA 
AAGCCCTTGG CCAGGCTGGT GGCTCCACTG GCCCCTGACA CGCAAGTGCT GGTCATACCC 
CTGCTGGACA AGGAGACTGG AACTGTGGCA GCCGTCATCT TGGTGCACTG TGGCCAGCTG 
AGTGACAGTG AGGAGCAGAG CCTGCAGGTG GTGGAGAAGC ATGCTCTGGT GGCCCTGCAG 
AGGGTGCAGG CCCTGCAGCA GCGCAGGCCT GAAGCTGTTC AGAACACCTC AGCGGATCCC 
TCTGAAGATC AAAAGGATGA GAAGGGATAC ACCGCCCATG ACCGAAAGAT CCTGCAACTG 
TGTGGAGAAC TCTATGACTT GGATGCCACT TCTCTGCAGC TCAAAGTCCT TCGATATCTT 
CAGCAGGAGA CACAGGCCAC TCACTGCTGC CTCCTGCTGG TGTCAGAGGA CAACCTGCAG 
CTTTCCTGCA AGGTCATTGG AGAGAAAGTG CTGGGAGAAG AGGTCAGCTT TCCATTGACC 
ATGGGACGCC TGGGCCAGGT GGTGGAGGAC AAACAGTGTA TCCAGTTGAA GGACCTAACC 
TCTGACGATG TGCAACAGCT ACAAAACATG TTGGGCTGTG AGCTTCGGGC TATGCTATGT 
GTCCCTGTCA TCAGTCGAGC CACTGACCAG GTGGTGGCCC TGGCTTGCGC CTTCAACAAG 
CTTGGAGGAG ACTTCTTCAC AGATGAGGAT GAACGTGCGA TCCAACACTG CTTCCACTAC 
ACAGGCACGG TGCTCACCAG TACCTTGGCC TTCCAGAAGG AGCAGAAGCT CAAGTGTGAG 
TGCCAGGCTC TTCTCCAAGT GGCAAAGAAC CTCTTCACCC ACCTGGATGA CGTCTCTGTC 
CTGCTACAGG AGATCATCAC AGAGGCCAGA AACCTCAGCA ACGCAGAGAT CTGCTCCGTG 
TTCCTGCTGG ATCAGAATGA GCTGGTTGCC AAGGTGTTCG ATGGCGGAGT AGTGGACGAT 
GAGAGTTATG AGATCCGCAT CCCTGCGGAC CAAGGCATCG CGGGCCACGT GGCGACCACG 
GGCCAGATCC TGAACATCCC AGATGCATAC GCCCATCCGC TTTTCTATCG CGGCGTAGAC 
GACAGCACTG GCTTCCGCAC GCGCAACATT CTCTGCTTCC CTATCAAGAA CGAGAACCAA 
GAGGTCATCG GTGTGGCTGA GCTAGTGAAC AAGATCAATG GGCCATGGTT CAGCAAATTT 
GATGAGGACC TGGCCACAGC CTTCTCCATC TACTGTGGCA TTAGCATCGC TCACTCTCTC 
CTATACAAAA AGGTGAATGA AGCCCAGTAC CGCAGCCACC TGGCCAACGA GATGATGATG 
TACCATATGA AGGTCTCTGA CGATGAATAC ACCAAACTTC TCCATGATGG CATCCAACCT 
GTGGCCGCCA TTGACTCCAA CTTTGCCAAC TTCACCTATA CTCCTCGGTC TCTGCCTGAG 
GACGATACTT CTATGGCCAT CCTGAGCATG CTGCAAGACA TGAACTTCAT CAATAACTAC 
AAAATTGACT GCCCAACTCT GGCCCGGTTC TGCCTGATGG TGAAGAAAGG CTACCGGGAT 
CCGCCCTACC ACAACTGGAT GCACGCCTTC TCTGTCTCTC ACTTTTGCTA CTTGCTCTAC 
AAGAATCTGG AGCTCTCCAA CTACCTCGAG GACATCGAGA TCTTTGCGTT GTTTATTTCC 
TGCATGTGTC ACGACCTGGA CCACAGAGGC ACAAACAACT CCTTCCAGGT GGCCTCGAAA 
TCTGTGCTGG CTGCACTCTA CAGCTCAGAG GGCTCTGTCA TGGAGAGGCA CCACTTTGCT 
CAGGCCATTG CTATCCTCAA CACCCATGGC TGTAATATCT TTGACCACTT CTCTCGGAAG 
GACTATCAGC GAATGCTGGA CCTGATGAGG GACATCATCT TGGCCACGGA CCTGGCACAC 
CACCTCCGCA TCTTCAAGGA CCTGCAGAAG ATGGCTGAAG TGGGTTATGA CCGAAACAAC 
AAGCAACACC ACAGGCTTCT TCTATGCCTC CTTATGACCT CCTGTGACCT CTCTGACCAG 
ACAAAGGGCT GGAAGACCAC CAGAAAGATT GCGGAGCTGA TCTACAAAGA GTTCTTCTCC 
CAAGGAGACT TGGAGAAGGC CATGGGCAAC CGACCAATGG AGATGATGGA TCGGGAGAAG 
GCCTACATCC CTGAACTTCA GATCAGCTTT ATGGAGCACA TCGCCATGCC CATCTACAAG 
CTGTTACAAG ACCTGTTCCC CAAGGCAGCA GAGCTGTATG AACGCGTGGC CTCCAATCGT 
GAGCACTGGA CCAAGGTGTC CCACAAGTTC ACCATCCGAG GCCTCCCCAG TAACAACTCG 
CTGGATTTCC TGGATGAGGA ATATGAGGTC CCTGATTTGG ACGTCACCAG GGCTCCTGTC 
AATGGCTGCT GCAGCCTCGA GGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_112341.1
CDS	70..2856
Translation	MVLVLHHILIAVVQFLRRGQQVFLKPDEPPPQPCADSLQDALLSLGAVIDIAGLRQAAKDALSAVLPKVETVYTYLVDGESRLVCEDPPHELPQEGKIREAVISRKRLSCDGLGPSDLLGKPLARLVAPLAPDTQVLVIPLLDKETGTVAAVILVHCGQLSDSEEQSLQVVEKHALVALQRVQALQQRRPEAVQNTSADPSEDQKDEKGYTAHDRKILQLCGELYDLDATSLQLKVLRYLQQETQATHCCLLLVSEDNLQLSCKVIGEKVLGEEVSFPLTMGRLGQVVEDKQCIQLKDLTSDDVQQLQNMLGCELRAMLCVPVISRATDQVVALACAFNKLGGDFFTDEDERAIQHCFHYTGTVLTSTLAFQKEQKLKCECQALLQVAKNLFTHLDDVSVLLQEIITEARNLSNAEICSVFLLDQNELVAKVFDGGVVDDESYEIRIPADQGIAGHVATTGQILNIPDAYAHPLFYRGVDDSTGFRTRNILCFPIKNENQEVIGVAELVNKINGPWFSKFDEDLATAFSIYCGISIAHSLLYKKVNEAQYRSHLANEMMMYHMKVSDDEYTKLLHDGIQPVAAIDSNFANFTYTPRSLPEDDTSMAILSMLQDMNFINNYKIDCPTLARFCLMVKKGYRDPPYHNWMHAFSVSHFCYLLYKNLELSNYLEDIEIFALFISCMCHDLDHRGTNNSFQVASKSVLAALYSSEGSVMERHHFAQAIAILNTHGCNIFDHFSRKDYQRMLDLMRDIILATDLAHHLRIFKDLQKMAEVGYDRNNKQHHRLLLCLLMTSCDLSDQTKGWKTTRKIAELIYKEFFSQGDLEKAMGNRPMEMMDREKAYIPELQISFMEHIAMPIYKLLQDLFPKAAELYERVASNREHWTKVSHKFTIRGLPSNNSLDFLDEEYEVPDLDVTRAPVNGCCSLEG

Target ORF information:

RefSeq Version	NM_031079.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosphodiesterase 2A (Pde2a), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031079.2

ORF Insert Sequence:

ATGGTCCTGG TGTTGCACCA CATCCTCATC GCTGTTGTCC AATTCCTCAG GCGGGGCCAG 
CAGGTCTTCC TCAAGCCGGA CGAACCGCCG CCGCAGCCAT GCGCCGACAG CCTGCAGGAT 
GCTTTGCTGA GCCTGGGCGC CGTTATCGAC ATCGCGGGCC TTCGACAGGC TGCCAAGGAT 
GCCCTCTCGG CCGTGCTCCC CAAAGTGGAG ACTGTCTACA CCTACCTGGT AGATGGGGAG 
TCCAGGCTGG TGTGTGAGGA CCCCCCTCAT GAGCTGCCAC AGGAAGGAAA AATTCGAGAG 
GCTGTGATTT CTCGGAAGCG GCTGAGCTGC GATGGGCTGG GACCTTCAGA CCTACTAGGA 
AAGCCCTTGG CCAGGCTGGT GGCTCCACTG GCCCCTGACA CGCAAGTGCT GGTCATACCC 
CTGCTGGACA AGGAGACTGG AACTGTGGCA GCCGTCATCT TGGTGCACTG TGGCCAGCTG 
AGTGACAGTG AGGAGCAGAG CCTGCAGGTG GTGGAGAAGC ATGCTCTGGT GGCCCTGCAG 
AGGGTGCAGG CCCTGCAGCA GCGCAGGCCT GAAGCTGTTC AGAACACCTC AGCGGATCCC 
TCTGAAGATC AAAAGGATGA GAAGGGATAC ACCGCCCATG ACCGAAAGAT CCTGCAACTG 
TGTGGAGAAC TCTATGACTT GGATGCCACT TCTCTGCAGC TCAAAGTCCT TCGATATCTT 
CAGCAGGAGA CACAGGCCAC TCACTGCTGC CTCCTGCTGG TGTCAGAGGA CAACCTGCAG 
CTTTCCTGCA AGGTCATTGG AGAGAAAGTG CTGGGAGAAG AGGTCAGCTT TCCATTGACC 
ATGGGACGCC TGGGCCAGGT GGTGGAGGAC AAACAGTGTA TCCAGTTGAA GGACCTAACC 
TCTGACGATG TGCAACAGCT ACAAAACATG TTGGGCTGTG AGCTTCGGGC TATGCTATGT 
GTCCCTGTCA TCAGTCGAGC CACTGACCAG GTGGTGGCCC TGGCTTGCGC CTTCAACAAG 
CTTGGAGGAG ACTTCTTCAC AGATGAGGAT GAACGTGCGA TCCAACACTG CTTCCACTAC 
ACAGGCACGG TGCTCACCAG TACCTTGGCC TTCCAGAAGG AGCAGAAGCT CAAGTGTGAG 
TGCCAGGCTC TTCTCCAAGT GGCAAAGAAC CTCTTCACCC ACCTGGATGA CGTCTCTGTC 
CTGCTACAGG AGATCATCAC AGAGGCCAGA AACCTCAGCA ACGCAGAGAT CTGCTCCGTG 
TTCCTGCTGG ATCAGAATGA GCTGGTTGCC AAGGTGTTCG ATGGCGGAGT AGTGGACGAT 
GAGAGTTATG AGATCCGCAT CCCTGCGGAC CAAGGCATCG CGGGCCACGT GGCGACCACG 
GGCCAGATCC TGAACATCCC AGATGCATAC GCCCATCCGC TTTTCTATCG CGGCGTAGAC 
GACAGCACTG GCTTCCGCAC GCGCAACATT CTCTGCTTCC CTATCAAGAA CGAGAACCAA 
GAGGTCATCG GTGTGGCTGA GCTAGTGAAC AAGATCAATG GGCCATGGTT CAGCAAATTT 
GATGAGGACC TGGCCACAGC CTTCTCCATC TACTGTGGCA TTAGCATCGC TCACTCTCTC 
CTATACAAAA AGGTGAATGA AGCCCAGTAC CGCAGCCACC TGGCCAACGA GATGATGATG 
TACCATATGA AGGTCTCTGA CGATGAATAC ACCAAACTTC TCCATGATGG CATCCAACCT 
GTGGCCGCCA TTGACTCCAA CTTTGCCAAC TTCACCTATA CTCCTCGGTC TCTGCCTGAG 
GACGATACTT CTATGGCCAT CCTGAGCATG CTGCAAGACA TGAACTTCAT CAATAACTAC 
AAAATTGACT GCCCAACTCT GGCCCGGTTC TGCCTGATGG TGAAGAAAGG CTACCGGGAT 
CCGCCCTACC ACAACTGGAT GCACGCCTTC TCTGTCTCTC ACTTTTGCTA CTTGCTCTAC 
AAGAATCTGG AGCTCTCCAA CTACCTCGAG GACATCGAGA TCTTTGCGTT GTTTATTTCC 
TGCATGTGTC ACGACCTGGA CCACAGAGGC ACAAACAACT CCTTCCAGGT GGCCTCGAAA 
TCTGTGCTGG CTGCACTCTA CAGCTCAGAG GGCTCTGTCA TGGAGAGGCA CCACTTTGCT 
CAGGCCATTG CTATCCTCAA CACCCATGGC TGTAATATCT TTGACCACTT CTCTCGGAAG 
GACTATCAGC GAATGCTGGA CCTGATGAGG GACATCATCT TGGCCACGGA CCTGGCACAC 
CACCTCCGCA TCTTCAAGGA CCTGCAGAAG ATGGCTGAAG TGGGTTATGA CCGAAACAAC 
AAGCAACACC ACAGGCTTCT TCTATGCCTC CTTATGACCT CCTGTGACCT CTCTGACCAG 
ACAAAGGGCT GGAAGACCAC CAGAAAGATT GCGGAGCTGA TCTACAAAGA GTTCTTCTCC 
CAAGGAGACT TGGAGAAGGC CATGGGCAAC CGACCAATGG AGATGATGGA TCGGGAGAAG 
GCCTACATCC CTGAACTTCA GATCAGCTTT ATGGAGCACA TCGCCATGCC CATCTACAAG 
CTGTTACAAG ACCTGTTCCC CAAGGCAGCA GAGCTGTATG AACGCGTGGC CTCCAATCGT 
GAGCACTGGA CCAAGGTGTC CCACAAGTTC ACCATCCGAG GCCTCCCCAG TAACAACTCG 
CTGGATTTCC TGGATGAGGA ATATGAGGTC CCTGATTTGG ACGTCACCAG GGCTCCTGTC 
AATGGCTGCT GCAGCCTCGA GGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

PDE2A2 regulates mitochondria morphology and apoptotic cell death via local modulation of cAMP/PKA signalling.
eLife6(2017 05)
Monterisi S,Lobo MJ,Livie C,Castle JC,Weinberger M,Baillie G,Surdo NC,Musheshe N,Stangherlin A,Gottlieb E,Maizels R,Bortolozzi M,Micaroni M,Zaccolo M

PDE2 activity differs in right and left rat ventricular myocardium and differentially regulates β2 adrenoceptor-mediated effects.
Experimental biology and medicine (Maywood, N.J.)240(9)1205-13(2015 Sep)
Soler F,Fernández-Belda F,Pérez-Schindler J,Handschin C,Fuente T,Hernandez-Cascales J

Cardiac Hypertrophy Is Inhibited by a Local Pool of cAMP Regulated by Phosphodiesterase 2.
Circulation research117(8)707-19(2015 Sep)
Zoccarato A,Surdo NC,Aronsen JM,Fields LA,Mancuso L,Dodoni G,Stangherlin A,Livie C,Jiang H,Sin YY,Gesellchen F,Terrin A,Baillie GS,Nicklin SA,Graham D,Szabo-Fresnais N,Krall J,Vandeput F,Movsesian M,Furlan L,Corsetti V,Hamilton G,Lefkimmiatis K,Sjaastad I,Zaccolo M

Efficacy of B-Type Natriuretic Peptide Is Coupled to Phosphodiesterase 2A in Cardiac Sympathetic Neurons.
Hypertension (Dallas, Tex. : 1979)66(1)190-8(2015 Jul)
Li D,Lu CJ,Hao G,Wright H,Woodward L,Liu K,Vergari E,Surdo NC,Herring N,Zaccolo M,Paterson DJ

Global genetic analysis in mice unveils central role for cilia in congenital heart disease.
Nature521(7553)520-4(2015 May)
Li Y,Klena NT,Gabriel GC,Liu X,Kim AJ,Lemke K,Chen Y,Chatterjee B,Devine W,Damerla RR,Chang C,Yagi H,San Agustin JT,Thahir M,Anderton S,Lawhead C,Vescovi A,Pratt H,Morgan J,Haynes L,Smith CL,Eppig JT,Reinholdt L,Francis R,Leatherbury L,Ganapathiraju MK,Tobita K,Pazour GJ,Lo CW

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Pde2a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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