Nfkb1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nfkb1
Entrez Gene ID	81736
Full Name	nuclear factor kappa B subunit 1
Synonyms	EBP-1,NF-kB
General protein information	Preferred Names nuclear factor kappa B subunit 1 Names nuclear factor NF-kappa-B p105 subunit DNA-binding factor KBF1 nuclear factor of kappa light chain gene enhancer in B-cells 1, p105 nuclear factor of kappa light polypeptide gene enhancer in B-cells 1, p105
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q43
Summary	gene encodes a 105 kD protein that can be processed to create a 50 kD protein; the larger protein is a Rel protein-specific transcription inhibitor and the smaller protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001276711.1	NP_001263640.1	nuclear factor NF-kappa-B p105 subunit
XM_006233358.3	XP_006233420.1	nuclear factor NF-kappa-B p105 subunit isoform X1
XM_006233360.3	XP_006233422.1	nuclear factor NF-kappa-B p105 subunit isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	NFKB1	XP_001168718.1
Canis lupus familiaris (dog)	NFKB1	NP_001003344.1
Bos taurus (cattle)	NFKB1	NP_001069877.1
Rattus norvegicus (Norway rat)	Nfkb1	NP_001263640.1
Gallus gallus (chicken)	NFKB1	NP_990465.1
Mus musculus (house mouse)	Nfkb1	NP_032715.2
Homo sapiens (human)	NFKB1	NP_003989.2
Xenopus tropicalis (tropical clawed frog)	nfkb1	XP_002934805.2
Macaca mulatta (Rhesus monkey)	NFKB1	XP_002804199.1

Gene Ontology
Bibliography

Related articles in PubMed

TNF-α enhances apoptosis by promoting chop expression in nucleus pulposus cells: role of the MAPK and NF-κB pathways.
Zhang J, Wang X, Liu H, Li Z, Chen F, Wang H, Zheng Z, Wang J
Journal of orthopaedic research : official publication of the Orthopaedic Research Society37(3)697-705(2019 03)

Celastrol ameliorates inflammatory pain and modulates HMGB1/NF-κB signaling pathway in dorsal root ganglion.
Zhang X, Zhao W, Liu X, Huang Z, Shan R, Huang C
Neuroscience letters69283-89(2019 01)

Ubiquitin Carboxyl Terminal Hydrolase L1 Attenuates TNF-α-Mediated Vascular Smooth Muscle Cell Migration Through Suppression of NF-κB Activation.
Gao X, Wu L, Wang K, Zhou X, Duan M, Wang X, Zhang Z, Liu X
International heart journal59(6)1409-1415(2018 Nov)

Tumor necrosis factor-alpha upregulated PHLPP1 through activating nuclear factor-kappa B during myocardial ischemia/reperfusion.
Tang B, Ma J, Ha X, Zhang Y, Xing Y
Life sciences207355-363(2018 Aug)

JMJD6 exerts function in neuropathic pain by regulating NF‑κB following peripheral nerve injury in rats.
Wen C, Xu M, Mo C, Cheng Z, Guo Q, Zhu X
International journal of molecular medicine42(1)633-642(2018 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Nfkb1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nfkb1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa12753	NM_001276711.1 Latest version!	Rattus norvegicus nuclear factor kappa B subunit 1 (Nfkb1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ORa25287	XM_006233358.3 Latest version!	Rattus norvegicus nuclear factor kappa B subunit 1 (Nfkb1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ORa25288	XM_006233360.3 Latest version!	Rattus norvegicus nuclear factor kappa B subunit 1 (Nfkb1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nfkb1 ( NM_001276711.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001263640.1 Rattus norvegicus nuclear factor kappa B subunit 1 (Nfkb1), mRNA.

Price & Availability↑

CloneID	ORa12753
Clone ID Related Accession (Same CDS sequence)	NM_001276711.1
Accession Version	NM_001276711.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2919bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1572796800000
Organism	Rattus norvegicus(Norway rat)
Product	nuclear factor NF-kappa-B p105 subunit
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07013508.1, AABR07013507.1 and AABR07072788.1. On or before Feb 28, 2013 this sequence version replaced XM_001075876.3, XM_342346.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCAGACG ACGATCCTTT CGGAACTGGG CAAATGTTTC ATTTGAATAC TGCTTTGACT 
CACTCCATAT TTAATGCAGA ATTATATTCA ACAGATATAC CACTGTCAAC AGATGGCCCA 
TACCTTCAAA TACTAGAGCA ACCGAAACAG AGAGGATTTC GATTCCGCTA CGTGTGCGAA 
GGCCCGTCCC ACGGAGGGCT TCCCGGTGCC TCCAGTGAGA AGAACAAGAA ATCTTACCCG 
CAGGTCAAAA TTTGCAACTA TGTGGGGCCT GCAAAGGTTA TCGTTCAGTT GGTCACAAAT 
GGAAAAAATA TTCACCTGCA CGCCCACAGC CTGGTGGGCA AGCACTGTGA GGACGGCATA 
TGCACCGTGA CAGCAGGACC CAAGGACATG GTGGTTGGCT TTGCAAACCT GGGAATACTT 
CACGTGACTA AGAAAAAGGT ATTCGAAACA CTGGAAGCAC GGATGACAGA GGCGTGTATA 
AGGGGCTATA ACCCTGGACT TCTGGTGCAT TCTGACCTTG CCTATCTACA GGCAGAAGGC 
GGAGGAGACC GGCAACTCAC AGACAGAGAG AAGGAGATCA TCCGCCAGGC AGCTCTGCAG 
CAGACCAAGG AGATGGACCT GAGTGTGGTG CGCCTCATGT TCACAGCCTT CCTCCCCGAC 
AGCACGGGCA GCTTCACTCG GAGACTGGAA CCTGTGGTGT CAGACGCCAT CTATGACAGC 
AAAGCCCCCA ACGCGTCCAA CCTGAAGATC GTAAGAATGG ACAGAACAGC AGGCTGCGTG 
ACGGGAGGGG AAGAAATCTA TCTTCTCTGT GACAAGGTCC AGAAAGATGA CATCCAGATT 
CGGTTTTATG AAGAGGAAGA AAATGGTGGA GTTTGGGAAG GATTTGGGGA CTTTTCCCCC 
ACGGATGTTC ACAGACAGTT TGCCATTGTC TTCAAAACAC CGAAGTATAA GGATGTCAAC 
ATTACAAAGC CAGCTTCCGT GTTTGTTCAG CTTCGGAGAA AATCGGATTT GGAAACTAGT 
GAACCGAAAC CCTTTCTCTA CTACCCCGAA ATCAAAGACA AGGAGGAAGT ACAGAGGAAA 
CGCCAGAAGC TCATGCCCAA CTTCTCCGAC AGTTTCGGCG GCGGCAGTGG AGCGGGAGCG 
GGTGGCGGAG GCATGTTCGG TAGTGGCGGT GGCGGTGGAG GGAGTACCGG AAGTCCTGGC 
CCAGGGTATG GCTTCCCGCA CTATGGATTT CCTGCTTACG GTGGGATTGC ATTCCATCCC 
GGAGCCACTA AATCCAACAC AGGCATCACC CATGGCACCA TAAACACCAA ATTTAAAAAC 
GAGCCTAGAG ATTGTGCCAA GAGTGACGAC AGGGAGATTC TGAATCCCCC TGAGAAAGAA 
ACACAAGGTG AGGGGCCCAG CCTGTTCATG GCCAGCACCA AGACCGAAGC AATTGCCCCG 
GCATCCACCA TGGAAGACAA GGAAGAGGAT GTGGGGTTTC AGGATAACCT CTTTCTTGAG 
AAGGCGCTGC AGCTCGCCAA GCGGCATGCC AACGCCCTCT TCGACTACGC GGTTACGGGA 
GATGTGAAGA TGCTGCTGGC GGTACAGCGC CATCTCACCG CGGTGCAGGA TGAGAACGGG 
GACAGTGTCC TACACTTAGC CATCATCCAC CTTCATGCTC AGCTCGTGAG GGATCTGCTA 
GAAGTGACAT CTGGTTCGAT CTCTGATGAC ATCATCAACA TGAGAAACGA TCTGTATCAG 
ACACCTTTGC ACTTGGCCGT GATCACCAAG CAGGAAGATG TGGTGGAGGA CTTGCTGAGG 
GTTGGGGCTG ACCTGAGTCT TCTGGACCGC TGGGGCAACT CTGTCCTGCA CCTAGCTGCC 
AAAGAAGGAC ATGATAAAAT CCTCGGGGTC CTACTCAAGA ACAGCAAGGC AGCACTCCTT 
ATCAACCACC CCAACGGGGA AGGTCTGAAT GCCATTCACA TAGCTGTGAT GAGCAACAGC 
CTGTCATGTC TGCAGCTGCT TGTGGCTGCT GGGGCAGAAG TCAACGCTCA GGAGCAGAAG 
TCTGGGCGCA CGGCACTGCA CCTGGCCGTG GAGTACGACA ACATCTCGTT GGCCGGCTGC 
CTGCTCCTGG AGGGTGACGC CCTCGTGGAC AGCACCACCT ACGATGGGAC GACACCTCTA 
CACATAGCAG CTGGAAGAGG GTCCACCAGG CTGGCAGCTC TTCTCAAAGC AGCAGGAGCA 
GACCCCCTGG TGGAGAACTT TGAGCCTCTC TATGACCTGG ACGACTCTTG GGAGAAGGCT 
GGAGAAGATG AGGGAGTAGT GCCAGGCACC ACACCCCTGG ACATGGCTGC CAACTGGCAG 
GTATTTGACA TACTAAATGG GAAACCATAT GAGCCAGTGT TCACATCTGA TGACATACTC 
CCACAAGGGG ACATTAAGCA GCTGACAGAA GACACGAGGC TGCAACTCTG CAAGCTCTTG 
GAAATTCCTG ATCCCGACAA GAACTGGGCC ACTCTGGCGC AGAAGTTAGG TCTGGGGATA 
TTGAACAATG CCTTCCGGCT GAGTCCCGCC CCTTCTAAAA CTCTTATGGA CAACTATGAG 
GTCTCTGGGG GGACCATCAA AGAGCTGGTG GAGGCCCTGC GACAGATGGG CTACACAGAG 
GCCATTGAAG TGATCCAGGC AGCCTTCCGC ACCCCGGAAA CCACAGCCTC CAGCCCCGTG 
ACCACTGCTC AGGCCCACTT GCTGCCTCTC TCGTCCTCCT CCACAAGACA GCACATAGAT 
GAGCTCCGAG ATAATGACAG CGTGTGTGAC AGTGGTGTGG AGACATCCTT CCGCAAACTC 
AGCTTCTCGG AGTCCCTCAC TGGAGATGGC CCACTGCTAT CTCTGAACAA AATGCCTCAC 
AATTATGGGC AGGATGGACC TATAGAAGGC AAAATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001263640.1
CDS	355..3273
Translation	MADDDPFGTGQMFHLNTALTHSIFNAELYSTDIPLSTDGPYLQILEQPKQRGFRFRYVCEGPSHGGLPGASSEKNKKSYPQVKICNYVGPAKVIVQLVTNGKNIHLHAHSLVGKHCEDGICTVTAGPKDMVVGFANLGILHVTKKKVFETLEARMTEACIRGYNPGLLVHSDLAYLQAEGGGDRQLTDREKEIIRQAALQQTKEMDLSVVRLMFTAFLPDSTGSFTRRLEPVVSDAIYDSKAPNASNLKIVRMDRTAGCVTGGEEIYLLCDKVQKDDIQIRFYEEEENGGVWEGFGDFSPTDVHRQFAIVFKTPKYKDVNITKPASVFVQLRRKSDLETSEPKPFLYYPEIKDKEEVQRKRQKLMPNFSDSFGGGSGAGAGGGGMFGSGGGGGGSTGSPGPGYGFPHYGFPAYGGIAFHPGATKSNTGITHGTINTKFKNEPRDCAKSDDREILNPPEKETQGEGPSLFMASTKTEAIAPASTMEDKEEDVGFQDNLFLEKALQLAKRHANALFDYAVTGDVKMLLAVQRHLTAVQDENGDSVLHLAIIHLHAQLVRDLLEVTSGSISDDIINMRNDLYQTPLHLAVITKQEDVVEDLLRVGADLSLLDRWGNSVLHLAAKEGHDKILGVLLKNSKAALLINHPNGEGLNAIHIAVMSNSLSCLQLLVAAGAEVNAQEQKSGRTALHLAVEYDNISLAGCLLLEGDALVDSTTYDGTTPLHIAAGRGSTRLAALLKAAGADPLVENFEPLYDLDDSWEKAGEDEGVVPGTTPLDMAANWQVFDILNGKPYEPVFTSDDILPQGDIKQLTEDTRLQLCKLLEIPDPDKNWATLAQKLGLGILNNAFRLSPAPSKTLMDNYEVSGGTIKELVEALRQMGYTEAIEVIQAAFRTPETTASSPVTTAQAHLLPLSSSSTRQHIDELRDNDSVCDSGVETSFRKLSFSESLTGDGPLLSLNKMPHNYGQDGPIEGKI

Target ORF information:

RefSeq Version	NM_001276711.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus nuclear factor kappa B subunit 1 (Nfkb1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001276711.1

ORF Insert Sequence:

ATGGCAGACG ACGATCCTTT CGGAACTGGG CAAATGTTTC ATTTGAATAC TGCTTTGACT 
CACTCCATAT TTAATGCAGA ATTATATTCA ACAGATATAC CACTGTCAAC AGATGGCCCA 
TACCTTCAAA TACTAGAGCA ACCGAAACAG AGAGGATTTC GATTCCGCTA CGTGTGCGAA 
GGCCCGTCCC ACGGAGGGCT TCCCGGTGCC TCCAGTGAGA AGAACAAGAA ATCTTACCCG 
CAGGTCAAAA TTTGCAACTA TGTGGGGCCT GCAAAGGTTA TCGTTCAGTT GGTCACAAAT 
GGAAAAAATA TTCACCTGCA CGCCCACAGC CTGGTGGGCA AGCACTGTGA GGACGGCATA 
TGCACCGTGA CAGCAGGACC CAAGGACATG GTGGTTGGCT TTGCAAACCT GGGAATACTT 
CACGTGACTA AGAAAAAGGT ATTCGAAACA CTGGAAGCAC GGATGACAGA GGCGTGTATA 
AGGGGCTATA ACCCTGGACT TCTGGTGCAT TCTGACCTTG CCTATCTACA GGCAGAAGGC 
GGAGGAGACC GGCAACTCAC AGACAGAGAG AAGGAGATCA TCCGCCAGGC AGCTCTGCAG 
CAGACCAAGG AGATGGACCT GAGTGTGGTG CGCCTCATGT TCACAGCCTT CCTCCCCGAC 
AGCACGGGCA GCTTCACTCG GAGACTGGAA CCTGTGGTGT CAGACGCCAT CTATGACAGC 
AAAGCCCCCA ACGCGTCCAA CCTGAAGATC GTAAGAATGG ACAGAACAGC AGGCTGCGTG 
ACGGGAGGGG AAGAAATCTA TCTTCTCTGT GACAAGGTCC AGAAAGATGA CATCCAGATT 
CGGTTTTATG AAGAGGAAGA AAATGGTGGA GTTTGGGAAG GATTTGGGGA CTTTTCCCCC 
ACGGATGTTC ACAGACAGTT TGCCATTGTC TTCAAAACAC CGAAGTATAA GGATGTCAAC 
ATTACAAAGC CAGCTTCCGT GTTTGTTCAG CTTCGGAGAA AATCGGATTT GGAAACTAGT 
GAACCGAAAC CCTTTCTCTA CTACCCCGAA ATCAAAGACA AGGAGGAAGT ACAGAGGAAA 
CGCCAGAAGC TCATGCCCAA CTTCTCCGAC AGTTTCGGCG GCGGCAGTGG AGCGGGAGCG 
GGTGGCGGAG GCATGTTCGG TAGTGGCGGT GGCGGTGGAG GGAGTACCGG AAGTCCTGGC 
CCAGGGTATG GCTTCCCGCA CTATGGATTT CCTGCTTACG GTGGGATTGC ATTCCATCCC 
GGAGCCACTA AATCCAACAC AGGCATCACC CATGGCACCA TAAACACCAA ATTTAAAAAC 
GAGCCTAGAG ATTGTGCCAA GAGTGACGAC AGGGAGATTC TGAATCCCCC TGAGAAAGAA 
ACACAAGGTG AGGGGCCCAG CCTGTTCATG GCCAGCACCA AGACCGAAGC AATTGCCCCG 
GCATCCACCA TGGAAGACAA GGAAGAGGAT GTGGGGTTTC AGGATAACCT CTTTCTTGAG 
AAGGCGCTGC AGCTCGCCAA GCGGCATGCC AACGCCCTCT TCGACTACGC GGTTACGGGA 
GATGTGAAGA TGCTGCTGGC GGTACAGCGC CATCTCACCG CGGTGCAGGA TGAGAACGGG 
GACAGTGTCC TACACTTAGC CATCATCCAC CTTCATGCTC AGCTCGTGAG GGATCTGCTA 
GAAGTGACAT CTGGTTCGAT CTCTGATGAC ATCATCAACA TGAGAAACGA TCTGTATCAG 
ACACCTTTGC ACTTGGCCGT GATCACCAAG CAGGAAGATG TGGTGGAGGA CTTGCTGAGG 
GTTGGGGCTG ACCTGAGTCT TCTGGACCGC TGGGGCAACT CTGTCCTGCA CCTAGCTGCC 
AAAGAAGGAC ATGATAAAAT CCTCGGGGTC CTACTCAAGA ACAGCAAGGC AGCACTCCTT 
ATCAACCACC CCAACGGGGA AGGTCTGAAT GCCATTCACA TAGCTGTGAT GAGCAACAGC 
CTGTCATGTC TGCAGCTGCT TGTGGCTGCT GGGGCAGAAG TCAACGCTCA GGAGCAGAAG 
TCTGGGCGCA CGGCACTGCA CCTGGCCGTG GAGTACGACA ACATCTCGTT GGCCGGCTGC 
CTGCTCCTGG AGGGTGACGC CCTCGTGGAC AGCACCACCT ACGATGGGAC GACACCTCTA 
CACATAGCAG CTGGAAGAGG GTCCACCAGG CTGGCAGCTC TTCTCAAAGC AGCAGGAGCA 
GACCCCCTGG TGGAGAACTT TGAGCCTCTC TATGACCTGG ACGACTCTTG GGAGAAGGCT 
GGAGAAGATG AGGGAGTAGT GCCAGGCACC ACACCCCTGG ACATGGCTGC CAACTGGCAG 
GTATTTGACA TACTAAATGG GAAACCATAT GAGCCAGTGT TCACATCTGA TGACATACTC 
CCACAAGGGG ACATTAAGCA GCTGACAGAA GACACGAGGC TGCAACTCTG CAAGCTCTTG 
GAAATTCCTG ATCCCGACAA GAACTGGGCC ACTCTGGCGC AGAAGTTAGG TCTGGGGATA 
TTGAACAATG CCTTCCGGCT GAGTCCCGCC CCTTCTAAAA CTCTTATGGA CAACTATGAG 
GTCTCTGGGG GGACCATCAA AGAGCTGGTG GAGGCCCTGC GACAGATGGG CTACACAGAG 
GCCATTGAAG TGATCCAGGC AGCCTTCCGC ACCCCGGAAA CCACAGCCTC CAGCCCCGTG 
ACCACTGCTC AGGCCCACTT GCTGCCTCTC TCGTCCTCCT CCACAAGACA GCACATAGAT 
GAGCTCCGAG ATAATGACAG CGTGTGTGAC AGTGGTGTGG AGACATCCTT CCGCAAACTC 
AGCTTCTCGG AGTCCCTCAC TGGAGATGGC CCACTGCTAT CTCTGAACAA AATGCCTCAC 
AATTATGGGC AGGATGGACC TATAGAAGGC AAAATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfkb1 ( XM_006233358.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006233420.1 Rattus norvegicus nuclear factor kappa B subunit 1 (Nfkb1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa25287
Clone ID Related Accession (Same CDS sequence)	XM_006233358.3
Accession Version	XM_006233358.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2922bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	nuclear factor NF-kappa-B p105 subunit isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006233358.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGACG ACGATCCTTT CGGAACTGGG CAAATGTTTC ATTTGAATAC TGCTTTGACT 
CACTCCATAT TTAATGCAGA ATTATATTCA ACAGATATAC CACTGTCAAC AGCAGATGGC 
CCATACCTTC AAATACTAGA GCAACCGAAA CAGAGAGGAT TTCGATTCCG CTACGTGTGC 
GAAGGCCCGT CCCACGGAGG GCTTCCCGGT GCCTCCAGTG AGAAGAACAA GAAATCTTAC 
CCGCAGGTCA AAATTTGCAA CTATGTGGGG CCTGCAAAGG TTATCGTTCA GTTGGTCACA 
AATGGAAAAA ATATTCACCT GCACGCCCAC AGCCTGGTGG GCAAGCACTG TGAGGACGGC 
ATATGCACCG TGACAGCAGG ACCCAAGGAC ATGGTGGTTG GCTTTGCAAA CCTGGGAATA 
CTTCACGTGA CTAAGAAAAA GGTATTCGAA ACACTGGAAG CACGGATGAC AGAGGCGTGT 
ATAAGGGGCT ATAACCCTGG ACTTCTGGTG CATTCTGACC TTGCCTATCT ACAGGCAGAA 
GGCGGAGGAG ACCGGCAACT CACAGACAGA GAGAAGGAGA TCATCCGCCA GGCAGCTCTG 
CAGCAGACCA AGGAGATGGA CCTGAGTGTG GTGCGCCTCA TGTTCACAGC CTTCCTCCCC 
GACAGCACGG GCAGCTTCAC TCGGAGACTG GAACCTGTGG TGTCAGACGC CATCTATGAC 
AGCAAAGCCC CCAACGCGTC CAACCTGAAG ATCGTAAGAA TGGACAGAAC AGCAGGCTGC 
GTGACGGGAG GGGAAGAAAT CTATCTTCTC TGTGACAAGG TCCAGAAAGA TGACATCCAG 
ATTCGGTTTT ATGAAGAGGA AGAAAATGGT GGAGTTTGGG AAGGATTTGG GGACTTTTCC 
CCCACGGATG TTCACAGACA GTTTGCCATT GTCTTCAAAA CACCGAAGTA TAAGGATGTC 
AACATTACAA AGCCAGCTTC CGTGTTTGTT CAGCTTCGGA GAAAATCGGA TTTGGAAACT 
AGTGAACCGA AACCCTTTCT CTACTACCCC GAAATCAAAG ACAAGGAGGA AGTACAGAGG 
AAACGCCAGA AGCTCATGCC CAACTTCTCC GACAGTTTCG GCGGCGGCAG TGGAGCGGGA 
GCGGGTGGCG GAGGCATGTT CGGTAGTGGC GGTGGCGGTG GAGGGAGTAC CGGAAGTCCT 
GGCCCAGGGT ATGGCTTCCC GCACTATGGA TTTCCTGCTT ACGGTGGGAT TGCATTCCAT 
CCCGGAGCCA CTAAATCCAA CACAGGCATC ACCCATGGCA CCATAAACAC CAAATTTAAA 
AACGAGCCTA GAGATTGTGC CAAGAGTGAC GACAGGGAGA TTCTGAATCC CCCTGAGAAA 
GAAACACAAG GTGAGGGGCC CAGCCTGTTC ATGGCCAGCA CCAAGACCGA AGCAATTGCC 
CCGGCATCCA CCATGGAAGA CAAGGAAGAG GATGTGGGGT TTCAGGATAA CCTCTTTCTT 
GAGAAGGCGC TGCAGCTCGC CAAGCGGCAT GCCAACGCCC TCTTCGACTA CGCGGTTACG 
GGAGATGTGA AGATGCTGCT GGCGGTACAG CGCCATCTCA CCGCGGTGCA GGATGAGAAC 
GGGGACAGTG TCCTACACTT AGCCATCATC CACCTTCATG CTCAGCTCGT GAGGGATCTG 
CTAGAAGTGA CATCTGGTTC GATCTCTGAT GACATCATCA ACATGAGAAA CGATCTGTAT 
CAGACACCTT TGCACTTGGC CGTGATCACC AAGCAGGAAG ATGTGGTGGA GGACTTGCTG 
AGGGTTGGGG CTGACCTGAG TCTTCTGGAC CGCTGGGGCA ACTCTGTCCT GCACCTAGCT 
GCCAAAGAAG GACATGATAA AATCCTCGGG GTCCTACTCA AGAACAGCAA GGCAGCACTC 
CTTATCAACC ACCCCAACGG GGAAGGTCTG AATGCCATTC ACATAGCTGT GATGAGCAAC 
AGCCTGTCAT GTCTGCAGCT GCTTGTGGCT GCTGGGGCAG AAGTCAACGC TCAGGAGCAG 
AAGTCTGGGC GCACGGCACT GCACCTGGCC GTGGAGTACG ACAACATCTC GTTGGCCGGC 
TGCCTGCTCC TGGAGGGTGA CGCCCTCGTG GACAGCACCA CCTACGATGG GACGACACCT 
CTACACATAG CAGCTGGAAG AGGGTCCACC AGGCTGGCAG CTCTTCTCAA AGCAGCAGGA 
GCAGACCCCC TGGTGGAGAA CTTTGAGCCT CTCTATGACC TGGACGACTC TTGGGAGAAG 
GCTGGAGAAG ATGAGGGAGT AGTGCCAGGC ACCACACCCC TGGACATGGC TGCCAACTGG 
CAGGTATTTG ACATACTAAA TGGGAAACCA TATGAGCCAG TGTTCACATC TGATGACATA 
CTCCCACAAG GGGACATTAA GCAGCTGACA GAAGACACGA GGCTGCAACT CTGCAAGCTC 
TTGGAAATTC CTGATCCCGA CAAGAACTGG GCCACTCTGG CGCAGAAGTT AGGTCTGGGG 
ATATTGAACA ATGCCTTCCG GCTGAGTCCC GCCCCTTCTA AAACTCTTAT GGACAACTAT 
GAGGTCTCTG GGGGGACCAT CAAAGAGCTG GTGGAGGCCC TGCGACAGAT GGGCTACACA 
GAGGCCATTG AAGTGATCCA GGCAGCCTTC CGCACCCCGG AAACCACAGC CTCCAGCCCC 
GTGACCACTG CTCAGGCCCA CTTGCTGCCT CTCTCGTCCT CCTCCACAAG ACAGCACATA 
GATGAGCTCC GAGATAATGA CAGCGTGTGT GACAGTGGTG TGGAGACATC CTTCCGCAAA 
CTCAGCTTCT CGGAGTCCCT CACTGGAGAT GGCCCACTGC TATCTCTGAA CAAAATGCCT 
CACAATTATG GGCAGGATGG ACCTATAGAA GGCAAAATTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006233420.1
CDS	350..3271
Translation	MADDDPFGTGQMFHLNTALTHSIFNAELYSTDIPLSTADGPYLQILEQPKQRGFRFRYVCEGPSHGGLPGASSEKNKKSYPQVKICNYVGPAKVIVQLVTNGKNIHLHAHSLVGKHCEDGICTVTAGPKDMVVGFANLGILHVTKKKVFETLEARMTEACIRGYNPGLLVHSDLAYLQAEGGGDRQLTDREKEIIRQAALQQTKEMDLSVVRLMFTAFLPDSTGSFTRRLEPVVSDAIYDSKAPNASNLKIVRMDRTAGCVTGGEEIYLLCDKVQKDDIQIRFYEEEENGGVWEGFGDFSPTDVHRQFAIVFKTPKYKDVNITKPASVFVQLRRKSDLETSEPKPFLYYPEIKDKEEVQRKRQKLMPNFSDSFGGGSGAGAGGGGMFGSGGGGGGSTGSPGPGYGFPHYGFPAYGGIAFHPGATKSNTGITHGTINTKFKNEPRDCAKSDDREILNPPEKETQGEGPSLFMASTKTEAIAPASTMEDKEEDVGFQDNLFLEKALQLAKRHANALFDYAVTGDVKMLLAVQRHLTAVQDENGDSVLHLAIIHLHAQLVRDLLEVTSGSISDDIINMRNDLYQTPLHLAVITKQEDVVEDLLRVGADLSLLDRWGNSVLHLAAKEGHDKILGVLLKNSKAALLINHPNGEGLNAIHIAVMSNSLSCLQLLVAAGAEVNAQEQKSGRTALHLAVEYDNISLAGCLLLEGDALVDSTTYDGTTPLHIAAGRGSTRLAALLKAAGADPLVENFEPLYDLDDSWEKAGEDEGVVPGTTPLDMAANWQVFDILNGKPYEPVFTSDDILPQGDIKQLTEDTRLQLCKLLEIPDPDKNWATLAQKLGLGILNNAFRLSPAPSKTLMDNYEVSGGTIKELVEALRQMGYTEAIEVIQAAFRTPETTASSPVTTAQAHLLPLSSSSTRQHIDELRDNDSVCDSGVETSFRKLSFSESLTGDGPLLSLNKMPHNYGQDGPIEGKI

Target ORF information:

RefSeq Version	XM_006233358.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus nuclear factor kappa B subunit 1 (Nfkb1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006233358.3

ORF Insert Sequence:

ATGGCAGACG ACGATCCTTT CGGAACTGGG CAAATGTTTC ATTTGAATAC TGCTTTGACT 
CACTCCATAT TTAATGCAGA ATTATATTCA ACAGATATAC CACTGTCAAC AGCAGATGGC 
CCATACCTTC AAATACTAGA GCAACCGAAA CAGAGAGGAT TTCGATTCCG CTACGTGTGC 
GAAGGCCCGT CCCACGGAGG GCTTCCCGGT GCCTCCAGTG AGAAGAACAA GAAATCTTAC 
CCGCAGGTCA AAATTTGCAA CTATGTGGGG CCTGCAAAGG TTATCGTTCA GTTGGTCACA 
AATGGAAAAA ATATTCACCT GCACGCCCAC AGCCTGGTGG GCAAGCACTG TGAGGACGGC 
ATATGCACCG TGACAGCAGG ACCCAAGGAC ATGGTGGTTG GCTTTGCAAA CCTGGGAATA 
CTTCACGTGA CTAAGAAAAA GGTATTCGAA ACACTGGAAG CACGGATGAC AGAGGCGTGT 
ATAAGGGGCT ATAACCCTGG ACTTCTGGTG CATTCTGACC TTGCCTATCT ACAGGCAGAA 
GGCGGAGGAG ACCGGCAACT CACAGACAGA GAGAAGGAGA TCATCCGCCA GGCAGCTCTG 
CAGCAGACCA AGGAGATGGA CCTGAGTGTG GTGCGCCTCA TGTTCACAGC CTTCCTCCCC 
GACAGCACGG GCAGCTTCAC TCGGAGACTG GAACCTGTGG TGTCAGACGC CATCTATGAC 
AGCAAAGCCC CCAACGCGTC CAACCTGAAG ATCGTAAGAA TGGACAGAAC AGCAGGCTGC 
GTGACGGGAG GGGAAGAAAT CTATCTTCTC TGTGACAAGG TCCAGAAAGA TGACATCCAG 
ATTCGGTTTT ATGAAGAGGA AGAAAATGGT GGAGTTTGGG AAGGATTTGG GGACTTTTCC 
CCCACGGATG TTCACAGACA GTTTGCCATT GTCTTCAAAA CACCGAAGTA TAAGGATGTC 
AACATTACAA AGCCAGCTTC CGTGTTTGTT CAGCTTCGGA GAAAATCGGA TTTGGAAACT 
AGTGAACCGA AACCCTTTCT CTACTACCCC GAAATCAAAG ACAAGGAGGA AGTACAGAGG 
AAACGCCAGA AGCTCATGCC CAACTTCTCC GACAGTTTCG GCGGCGGCAG TGGAGCGGGA 
GCGGGTGGCG GAGGCATGTT CGGTAGTGGC GGTGGCGGTG GAGGGAGTAC CGGAAGTCCT 
GGCCCAGGGT ATGGCTTCCC GCACTATGGA TTTCCTGCTT ACGGTGGGAT TGCATTCCAT 
CCCGGAGCCA CTAAATCCAA CACAGGCATC ACCCATGGCA CCATAAACAC CAAATTTAAA 
AACGAGCCTA GAGATTGTGC CAAGAGTGAC GACAGGGAGA TTCTGAATCC CCCTGAGAAA 
GAAACACAAG GTGAGGGGCC CAGCCTGTTC ATGGCCAGCA CCAAGACCGA AGCAATTGCC 
CCGGCATCCA CCATGGAAGA CAAGGAAGAG GATGTGGGGT TTCAGGATAA CCTCTTTCTT 
GAGAAGGCGC TGCAGCTCGC CAAGCGGCAT GCCAACGCCC TCTTCGACTA CGCGGTTACG 
GGAGATGTGA AGATGCTGCT GGCGGTACAG CGCCATCTCA CCGCGGTGCA GGATGAGAAC 
GGGGACAGTG TCCTACACTT AGCCATCATC CACCTTCATG CTCAGCTCGT GAGGGATCTG 
CTAGAAGTGA CATCTGGTTC GATCTCTGAT GACATCATCA ACATGAGAAA CGATCTGTAT 
CAGACACCTT TGCACTTGGC CGTGATCACC AAGCAGGAAG ATGTGGTGGA GGACTTGCTG 
AGGGTTGGGG CTGACCTGAG TCTTCTGGAC CGCTGGGGCA ACTCTGTCCT GCACCTAGCT 
GCCAAAGAAG GACATGATAA AATCCTCGGG GTCCTACTCA AGAACAGCAA GGCAGCACTC 
CTTATCAACC ACCCCAACGG GGAAGGTCTG AATGCCATTC ACATAGCTGT GATGAGCAAC 
AGCCTGTCAT GTCTGCAGCT GCTTGTGGCT GCTGGGGCAG AAGTCAACGC TCAGGAGCAG 
AAGTCTGGGC GCACGGCACT GCACCTGGCC GTGGAGTACG ACAACATCTC GTTGGCCGGC 
TGCCTGCTCC TGGAGGGTGA CGCCCTCGTG GACAGCACCA CCTACGATGG GACGACACCT 
CTACACATAG CAGCTGGAAG AGGGTCCACC AGGCTGGCAG CTCTTCTCAA AGCAGCAGGA 
GCAGACCCCC TGGTGGAGAA CTTTGAGCCT CTCTATGACC TGGACGACTC TTGGGAGAAG 
GCTGGAGAAG ATGAGGGAGT AGTGCCAGGC ACCACACCCC TGGACATGGC TGCCAACTGG 
CAGGTATTTG ACATACTAAA TGGGAAACCA TATGAGCCAG TGTTCACATC TGATGACATA 
CTCCCACAAG GGGACATTAA GCAGCTGACA GAAGACACGA GGCTGCAACT CTGCAAGCTC 
TTGGAAATTC CTGATCCCGA CAAGAACTGG GCCACTCTGG CGCAGAAGTT AGGTCTGGGG 
ATATTGAACA ATGCCTTCCG GCTGAGTCCC GCCCCTTCTA AAACTCTTAT GGACAACTAT 
GAGGTCTCTG GGGGGACCAT CAAAGAGCTG GTGGAGGCCC TGCGACAGAT GGGCTACACA 
GAGGCCATTG AAGTGATCCA GGCAGCCTTC CGCACCCCGG AAACCACAGC CTCCAGCCCC 
GTGACCACTG CTCAGGCCCA CTTGCTGCCT CTCTCGTCCT CCTCCACAAG ACAGCACATA 
GATGAGCTCC GAGATAATGA CAGCGTGTGT GACAGTGGTG TGGAGACATC CTTCCGCAAA 
CTCAGCTTCT CGGAGTCCCT CACTGGAGAT GGCCCACTGC TATCTCTGAA CAAAATGCCT 
CACAATTATG GGCAGGATGG ACCTATAGAA GGCAAAATTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfkb1 ( XM_006233360.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006233422.1 Rattus norvegicus nuclear factor kappa B subunit 1 (Nfkb1), transcript variant X2, mRNA.

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CloneID	ORa25288
Clone ID Related Accession (Same CDS sequence)	XM_006233360.3
Accession Version	XM_006233360.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2691bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	nuclear factor NF-kappa-B p105 subunit isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006233360.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGACG ACGATCCTTT CGGAACTGGG CAAATGTTTC ATTTGAATAC TGCTTTGACT 
CACTCCATAT TTAATGCAGA ATTATATTCA ACAGATATAC CACTGTCAAC AGCAGATGGC 
CCATACCTTC AAATACTAGA GCAACCGAAA CAGAGAGGAT TTCGATTCCG CTACGTGTGC 
GAAGGCCCGT CCCACGGAGG GCTTCCCGGT GCCTCCAGTG AGAAGAACAA GAAATCTTAC 
CCGCAGGTCA AAATTTGCAA CTATGTGGGG CCTGCAAAGG TTATCGTTCA GTTGGTCACA 
AATGGAAAAA ATATTCACCT GCACGCCCAC AGCCTGGTGG GCAAGCACTG TGAGGACGGC 
ATATGCACCG TGACAGCAGG ACCCAAGGAC ATGGTGGTTG GCTTTGCAAA CCTGGGAATA 
CTTCACGTGA CTAAGAAAAA GGTATTCGAA ACACTGGAAG CACGGATGAC AGAGGCGTGT 
ATAAGGGGCT ATAACCCTGG ACTTCTGGTG CATTCTGACC TTGCCTATCT ACAGGCAGAA 
GGCGGAGGAG ACCGGCAACT CACAGACAGA GAGAAGGAGA TCATCCGCCA GGCAGCTCTG 
CAGCAGACCA AGGAGATGGA CCTGAGTGTG GTGCGCCTCA TGTTCACAGC CTTCCTCCCC 
GACAGCACGG GCAGCTTCAC TCGGAGACTG GAACCTGTGG TGTCAGACGC CATCTATGAC 
AGCAAAGCCC CCAACGCGTC CAACCTGAAG ATCGTAAGAA TGGACAGAAC AGCAGGCTGC 
GTGACGGGAG GGGAAGAAAT CTATCTTCTC TGTGACAAGG TCCAGAAAGA TGACATCCAG 
ATTCGGTTTT ATGAAGAGGA AGAAAATGGT GGAGTTTGGG AAGGATTTGG GGACTTTTCC 
CCCACGGATG TTCACAGACA GTTTGCCATT GTCTTCAAAA CACCGAAGTA TAAGGATGTC 
AACATTACAA AGCCAGCTTC CGTGTTTGTT CAGCTTCGGA GAAAATCGGA TTTGGAAACT 
AGTGAACCGA AACCCTTTCT CTACTACCCC GAAATCAAAG ACAAGGAGGA AGTACAGAGG 
AAACGCCAGA AGCTCATGCC CAACTTCTCC GACAGTTTCG GCGGCGGCAG TGGAGCGGGA 
GCGGGTGGCG GAGGCATGTT CGGTAGTGGC GGTGGCGGTG GAGGGAGTAC CGGAAGTCCT 
GGCCCAGGGT ATGGCTTCCC GCACTATGGA TTTCCTGCTT ACGGTGGGAT TGCATTCCAT 
CCCGGAGCCA CTAAATCCAA CACAGGCATC ACCCATGGCA CCATAAACAC CAAATTTAAA 
AACGAGCCTA GAGATTGTGC CAAGAGTGAC GACAGGGAGA TTCTGAATCC CCCTGAGAAA 
GAAACACAAG GTGAGGGGCC CAGCCTGTTC ATGGCCAGCA CCAAGACCGA AGCAATTGCC 
CCGGCATCCA CCATGGAAGA CAAGGAAGAG GATGTGGGGT TTCAGGATAA CCTCTTTCTT 
GAGAAGGCGC TGCAGCTCGC CAAGCGGCAT GCCAACGCCC TCTTCGACTA CGCGGTTACG 
GGAGATGTGA AGATGCTGCT GGCGGTACAG CGCCATCTCA CCGCGGTGCA GGATGAGAAC 
GGGGACAGTG TCCTACACTT AGCCATCATC CACCTTCATG CTCAGCTCGT GAGGGATCTG 
CTAGAAGTGA CATCTGGTTC GATCTCTGAT GACATCATCA ACATGAGAAA CGATCTGTAT 
CAGACACCTT TGCACTTGGC CGTGATCACC AAGCAGGAAG ATGTGGTGGA GGACTTGCTG 
AGGGTTGGGG CTGACCTGAG TCTTCTGGAC CGCTGGGGCA ACTCTGTCCT GCACCTAGCT 
GCCAAAGAAG GACATGATAA AATCCTCGGG GTCCTACTCA AGAACAGCAA GGCAGCACTC 
CTTATCAACC ACCCCAACGG GGAAGGTCTG AATGCCATTC ACATAGCTGT GATGAGCAAC 
AGCCTGTCAT GTCTGCAGCT GCTTGTGGCT GCTGGGGCAG AAGTCAACGC TCAGGAGCAG 
AAGTCTGGGC GCACGGCACT GCACCTGGCC GTGGAGTACG ACAACATCTC GTTGGCCGGC 
TGCCTGCTCC TGGAGGGTGA CGCCCTCGTG GACAGCACCA CCTACGATGG GACGACACCT 
CTACACATAG CAGCTGGAAG AGGGTCCACC AGGCTGGCAG CTCTTCTCAA AGCAGCAGGA 
GCAGACCCCC TGGTGGAGAA CTTTGAGCCT CTCTATGACC TGGACGACTC TTGGGAGAAG 
GCTGGAGAAG ATGAGGGAGT AGTGCCAGGC ACCACACCCC TGGACATGGC TGCCAACTGG 
CAGGTATTTG ACATACTAAA TGGGAAACCA TATGAGCCAG TGTTCACATC TGATGACATA 
CTCCCACAAG GGGACATTAA GCAGCTGACA GAAGACACGA GGCTGCAACT CTGCAAGCTC 
TTGGAAATTC CTGATCCCGA CAAGAACTGG GCCACTCTGG CGCAGAAGTT AGGTCTGGGG 
ATATTGAACA ATGCCTTCCG GCTGAGTCCC GCCCCTTCTA AAACTCTTAT GGACAACTAT 
GAGATGAGCT CCGAGATAAT GACAGCGTGT GTGACAGTGG TGTGGAGACA TCCTTCCGCA 
AACTCAGCTT CTCGGAGTCC CTCACTGGAG ATGGCCCACT GCTATCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006233422.1
CDS	350..3040
Translation	MADDDPFGTGQMFHLNTALTHSIFNAELYSTDIPLSTADGPYLQILEQPKQRGFRFRYVCEGPSHGGLPGASSEKNKKSYPQVKICNYVGPAKVIVQLVTNGKNIHLHAHSLVGKHCEDGICTVTAGPKDMVVGFANLGILHVTKKKVFETLEARMTEACIRGYNPGLLVHSDLAYLQAEGGGDRQLTDREKEIIRQAALQQTKEMDLSVVRLMFTAFLPDSTGSFTRRLEPVVSDAIYDSKAPNASNLKIVRMDRTAGCVTGGEEIYLLCDKVQKDDIQIRFYEEEENGGVWEGFGDFSPTDVHRQFAIVFKTPKYKDVNITKPASVFVQLRRKSDLETSEPKPFLYYPEIKDKEEVQRKRQKLMPNFSDSFGGGSGAGAGGGGMFGSGGGGGGSTGSPGPGYGFPHYGFPAYGGIAFHPGATKSNTGITHGTINTKFKNEPRDCAKSDDREILNPPEKETQGEGPSLFMASTKTEAIAPASTMEDKEEDVGFQDNLFLEKALQLAKRHANALFDYAVTGDVKMLLAVQRHLTAVQDENGDSVLHLAIIHLHAQLVRDLLEVTSGSISDDIINMRNDLYQTPLHLAVITKQEDVVEDLLRVGADLSLLDRWGNSVLHLAAKEGHDKILGVLLKNSKAALLINHPNGEGLNAIHIAVMSNSLSCLQLLVAAGAEVNAQEQKSGRTALHLAVEYDNISLAGCLLLEGDALVDSTTYDGTTPLHIAAGRGSTRLAALLKAAGADPLVENFEPLYDLDDSWEKAGEDEGVVPGTTPLDMAANWQVFDILNGKPYEPVFTSDDILPQGDIKQLTEDTRLQLCKLLEIPDPDKNWATLAQKLGLGILNNAFRLSPAPSKTLMDNYEMSSEIMTACVTVVWRHPSANSASRSPSLEMAHCYL

Target ORF information:

RefSeq Version	XM_006233360.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus nuclear factor kappa B subunit 1 (Nfkb1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006233360.3

ORF Insert Sequence:

ATGGCAGACG ACGATCCTTT CGGAACTGGG CAAATGTTTC ATTTGAATAC TGCTTTGACT 
CACTCCATAT TTAATGCAGA ATTATATTCA ACAGATATAC CACTGTCAAC AGCAGATGGC 
CCATACCTTC AAATACTAGA GCAACCGAAA CAGAGAGGAT TTCGATTCCG CTACGTGTGC 
GAAGGCCCGT CCCACGGAGG GCTTCCCGGT GCCTCCAGTG AGAAGAACAA GAAATCTTAC 
CCGCAGGTCA AAATTTGCAA CTATGTGGGG CCTGCAAAGG TTATCGTTCA GTTGGTCACA 
AATGGAAAAA ATATTCACCT GCACGCCCAC AGCCTGGTGG GCAAGCACTG TGAGGACGGC 
ATATGCACCG TGACAGCAGG ACCCAAGGAC ATGGTGGTTG GCTTTGCAAA CCTGGGAATA 
CTTCACGTGA CTAAGAAAAA GGTATTCGAA ACACTGGAAG CACGGATGAC AGAGGCGTGT 
ATAAGGGGCT ATAACCCTGG ACTTCTGGTG CATTCTGACC TTGCCTATCT ACAGGCAGAA 
GGCGGAGGAG ACCGGCAACT CACAGACAGA GAGAAGGAGA TCATCCGCCA GGCAGCTCTG 
CAGCAGACCA AGGAGATGGA CCTGAGTGTG GTGCGCCTCA TGTTCACAGC CTTCCTCCCC 
GACAGCACGG GCAGCTTCAC TCGGAGACTG GAACCTGTGG TGTCAGACGC CATCTATGAC 
AGCAAAGCCC CCAACGCGTC CAACCTGAAG ATCGTAAGAA TGGACAGAAC AGCAGGCTGC 
GTGACGGGAG GGGAAGAAAT CTATCTTCTC TGTGACAAGG TCCAGAAAGA TGACATCCAG 
ATTCGGTTTT ATGAAGAGGA AGAAAATGGT GGAGTTTGGG AAGGATTTGG GGACTTTTCC 
CCCACGGATG TTCACAGACA GTTTGCCATT GTCTTCAAAA CACCGAAGTA TAAGGATGTC 
AACATTACAA AGCCAGCTTC CGTGTTTGTT CAGCTTCGGA GAAAATCGGA TTTGGAAACT 
AGTGAACCGA AACCCTTTCT CTACTACCCC GAAATCAAAG ACAAGGAGGA AGTACAGAGG 
AAACGCCAGA AGCTCATGCC CAACTTCTCC GACAGTTTCG GCGGCGGCAG TGGAGCGGGA 
GCGGGTGGCG GAGGCATGTT CGGTAGTGGC GGTGGCGGTG GAGGGAGTAC CGGAAGTCCT 
GGCCCAGGGT ATGGCTTCCC GCACTATGGA TTTCCTGCTT ACGGTGGGAT TGCATTCCAT 
CCCGGAGCCA CTAAATCCAA CACAGGCATC ACCCATGGCA CCATAAACAC CAAATTTAAA 
AACGAGCCTA GAGATTGTGC CAAGAGTGAC GACAGGGAGA TTCTGAATCC CCCTGAGAAA 
GAAACACAAG GTGAGGGGCC CAGCCTGTTC ATGGCCAGCA CCAAGACCGA AGCAATTGCC 
CCGGCATCCA CCATGGAAGA CAAGGAAGAG GATGTGGGGT TTCAGGATAA CCTCTTTCTT 
GAGAAGGCGC TGCAGCTCGC CAAGCGGCAT GCCAACGCCC TCTTCGACTA CGCGGTTACG 
GGAGATGTGA AGATGCTGCT GGCGGTACAG CGCCATCTCA CCGCGGTGCA GGATGAGAAC 
GGGGACAGTG TCCTACACTT AGCCATCATC CACCTTCATG CTCAGCTCGT GAGGGATCTG 
CTAGAAGTGA CATCTGGTTC GATCTCTGAT GACATCATCA ACATGAGAAA CGATCTGTAT 
CAGACACCTT TGCACTTGGC CGTGATCACC AAGCAGGAAG ATGTGGTGGA GGACTTGCTG 
AGGGTTGGGG CTGACCTGAG TCTTCTGGAC CGCTGGGGCA ACTCTGTCCT GCACCTAGCT 
GCCAAAGAAG GACATGATAA AATCCTCGGG GTCCTACTCA AGAACAGCAA GGCAGCACTC 
CTTATCAACC ACCCCAACGG GGAAGGTCTG AATGCCATTC ACATAGCTGT GATGAGCAAC 
AGCCTGTCAT GTCTGCAGCT GCTTGTGGCT GCTGGGGCAG AAGTCAACGC TCAGGAGCAG 
AAGTCTGGGC GCACGGCACT GCACCTGGCC GTGGAGTACG ACAACATCTC GTTGGCCGGC 
TGCCTGCTCC TGGAGGGTGA CGCCCTCGTG GACAGCACCA CCTACGATGG GACGACACCT 
CTACACATAG CAGCTGGAAG AGGGTCCACC AGGCTGGCAG CTCTTCTCAA AGCAGCAGGA 
GCAGACCCCC TGGTGGAGAA CTTTGAGCCT CTCTATGACC TGGACGACTC TTGGGAGAAG 
GCTGGAGAAG ATGAGGGAGT AGTGCCAGGC ACCACACCCC TGGACATGGC TGCCAACTGG 
CAGGTATTTG ACATACTAAA TGGGAAACCA TATGAGCCAG TGTTCACATC TGATGACATA 
CTCCCACAAG GGGACATTAA GCAGCTGACA GAAGACACGA GGCTGCAACT CTGCAAGCTC 
TTGGAAATTC CTGATCCCGA CAAGAACTGG GCCACTCTGG CGCAGAAGTT AGGTCTGGGG 
ATATTGAACA ATGCCTTCCG GCTGAGTCCC GCCCCTTCTA AAACTCTTAT GGACAACTAT 
GAGATGAGCT CCGAGATAAT GACAGCGTGT GTGACAGTGG TGTGGAGACA TCCTTCCGCA 
AACTCAGCTT CTCGGAGTCC CTCACTGGAG ATGGCCCACT GCTATCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

TNF-α enhances apoptosis by promoting chop expression in nucleus pulposus cells: role of the MAPK and NF-κB pathways.
Journal of orthopaedic research : official publication of the Orthopaedic Research Society37(3)697-705(2019 03)
Zhang J,Wang X,Liu H,Li Z,Chen F,Wang H,Zheng Z,Wang J

Celastrol ameliorates inflammatory pain and modulates HMGB1/NF-κB signaling pathway in dorsal root ganglion.
Neuroscience letters69283-89(2019 01)
Zhang X,Zhao W,Liu X,Huang Z,Shan R,Huang C

Ubiquitin Carboxyl Terminal Hydrolase L1 Attenuates TNF-α-Mediated Vascular Smooth Muscle Cell Migration Through Suppression of NF-κB Activation.
International heart journal59(6)1409-1415(2018 Nov)
Gao X,Wu L,Wang K,Zhou X,Duan M,Wang X,Zhang Z,Liu X

Tumor necrosis factor-alpha upregulated PHLPP1 through activating nuclear factor-kappa B during myocardial ischemia/reperfusion.
Life sciences207355-363(2018 Aug)
Tang B,Ma J,Ha X,Zhang Y,Xing Y

JMJD6 exerts function in neuropathic pain by regulating NF‑κB following peripheral nerve injury in rats.
International journal of molecular medicine42(1)633-642(2018 Jul)
Wen C,Xu M,Mo C,Cheng Z,Guo Q,Zhu X

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Nfkb1 cDNA ORF clone, Rattus norvegicus(Norway rat)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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