Trim23 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Trim23
Entrez Gene ID	81002
Full Name	tripartite motif-containing 23
Synonyms	Ard1,Arfd1
General protein information	Preferred Names tripartite motif-containing 23 Names E3 ubiquitin-protein ligase TRIM23 ADP-ribosylation factor domain protein 1, 64kD ADP-ribosylation factor domain-containing protein 1 GTP-binding protein ARD-1 RING-type E3 ubiquitin transferase TRIM23 nucleotide binding protein tripartite motif protein 23 tripartite motif-containing protein 23
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q12
Summary	a guanine nucleotide-binding protein that may function as a ADP-ribosylation factor [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_008760684.2	XP_008758906.1	E3 ubiquitin-protein ligase TRIM23 isoform X2
XM_008760683.2	XP_008758905.1	E3 ubiquitin-protein ligase TRIM23 isoform X1
NM_001100637.1	NP_001094107.1	E3 ubiquitin-protein ligase TRIM23

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	TRIM23	XP_001163376.1
Macaca mulatta (Rhesus monkey)	TRIM23	XP_001085950.2
Rattus norvegicus (Norway rat)	Trim23	NP_001094107.1
Gallus gallus (chicken)	TRIM23	XP_424752.2
Bos taurus (cattle)	TRIM23	NP_001073772.1
Xenopus tropicalis (tropical clawed frog)	trim23	XP_002934252.2
Caenorhabditis elegans (roundworm)	arc-1	NP_496089.3
Homo sapiens (human)	TRIM23	NP_001647.1
Canis lupus familiaris (dog)	TRIM23	XP_544360.1
Mus musculus (house mouse)	Trim23	NP_109656.1
Danio rerio (zebrafish)	trim23	NP_001070112.1

Gene Ontology
Bibliography

Related articles in PubMed

Systematic analysis of dimeric E3-RING interactions reveals increased combinatorial complexity in human ubiquitination networks.
Woodsmith J, Jenn RC, Sanderson CM
Molecular & cellular proteomics : MCP11(7)M111.016162(2012 Jul)

E3 ubiquitin ligase activity of the trifunctional ARD1 (ADP-ribosylation factor domain protein 1).
Vichi A, Payne DM, Pacheco-Rodriguez G, Moss J, Vaughan M
Proceedings of the National Academy of Sciences of the United States of America102(6)1945-50(2005 Feb)

The tripartite motif family identifies cell compartments.
Reymond A, Meroni G, Fantozzi A, Merla G, Cairo S, Luzi L, Riganelli D, Zanaria E, Messali S, Cainarca S, Guffanti A, Minucci S, Pelicci PG, Ballabio A
The EMBO journal20(9)2140-51(2001 May)

Localization of ADP-ribosylation factor domain protein 1 (ARD1) in lysosomes and Golgi apparatus.
Vitale N, Horiba K, Ferrans VJ, Moss J, Vaughan M
Proceedings of the National Academy of Sciences of the United States of America95(15)8613-8(1998 Jul)

ARD1, a 64-kDa bifunctional protein containing an 18-kDa GTP-binding ADP-ribosylation factor domain and a 46-kDa GTPase-activating domain.
Vitale N, Moss J, Vaughan M
Proceedings of the National Academy of Sciences of the United States of America93(5)1941-4(1996 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Trim23 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Trim23 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa04253	NM_001100637.1 Latest version!	Rattus norvegicus tripartite motif-containing 23 (Trim23), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ORa43697	XM_008760683.2 Latest version!	Rattus norvegicus tripartite motif-containing 23 (Trim23), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ORa43698	XM_008760684.2 Latest version!	Rattus norvegicus tripartite motif-containing 23 (Trim23), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Trim23 ( NM_001100637.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001094107.1 Rattus norvegicus tripartite motif-containing 23 (Trim23), mRNA.

Price & Availability↑

CloneID	ORa04253
Clone ID Related Accession (Same CDS sequence)	NM_001100637.1
Accession Version	NM_001100637.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1725bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1571500800000
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase TRIM23
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CB696360.1, FM052834.1, L04760.1, CK470479.1, FM085917.1, DV726798.1 and AA965268.1. On or before May 22, 2010 this sequence version replaced XM_342183.4, XM_001064069.2, XM_001064021.1. ##Evidence-Data-START## Transcript exon combination :: L04760.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGCCC TCGCTGTAAA CAAGCCCGGA GCAGGAGTAG ACAGTGGGCG GCAGGGCAGC 
CGGGGGACGG CTGTGGTGAA GGTTCTGGAG TGTGGTGTTT GTGAAGATGT CTTCTCTCTG 
CAAGGAGATA AAGTTCCTCG CCTTCTCCTT TGTGGCCACA CAGTCTGTCA TGACTGTCTC 
TCTCGCTTGC CTCTGCATGG AAGAGCAATA CGTTGCCCAT TTGATCGACA AGTGACAGAC 
CTAGGAGATT CAGGTGTCTG GGGTTTGAAA AAAAATTTTG CTTTACTGGA GCTTTTAGAA 
CGTTTGCAAA ATGGACATAT TGGTCAGTAT GGAGCTGCAG AAGAAGCCCT TGGGATATCT 
GGAGAGAGTA TCATTCGCTG TGATGAAGAT GAAGCTCACG TTGCATCTGT GTACTGCACT 
GTGTGTGCGA CTCACTTGTG CTCAGAGTGT TCTCAAGTCA CTCATTCTAC AAAGACTCTG 
GCCAAGCATA GGCGAGTGCC TCTAGCTGAT AAACCTCATG AGAAGACCAT GTGCTGTCAG 
CACCAGGTAC ATGCCATTGA GTTTGTTTGC TTGGAAGAAG CTTGTCAAAC TAGTCCTCTC 
ATGTGCTGTG TCTGCAAAGA ATATGGAAAA CACCAGGGTC ACAAGCACTC AGTACTGGAG 
CCAGAAGCTA ACCAGATCCG AGCGTCAATT TTAGACATGG CTCACTGCAT ACGAACCTTC 
ACTGAGGAGA TCTCAGATTA TTCCAGAAAA TTAGTTGGGA TTGTTCAGCA CATTGAAGGA 
GGAGAACAAA TAGTGGAAGA TGGAATTGGA ATGGCTCACA CGGAACACGT CCCAGGTACT 
GCAGAGAATG CACGATCATG TGTCAGAGCT TATTTTTCTG ATTTGCATGA AACTCTTTGT 
CGTCAAGAAG AAATGGCTCT CAGTGTTGTT GATGCCCATG TTCGAGAAAA ACTGATTTGG 
CTTAGGCAGC AACAAGAGGA TATGACTATT CTCTTGTCCC AGGTTTCAAC AGCCTGTCTC 
CATTGTGAAA AGACTTTGCA GCAGGATGAT TGCAGAGTTG TCTTGGCAAA ACAAGAAATC 
ACAAGATTAT TAGAAACACT GCAGAAACAG CAGCAGCAGT TTACAGAAGT TGCAGATCAT 
ATTCAGTTGG ATGCCAGTAT TCCAGTCACT TTTACAAAGG ACAACAGAGT TTATATTGGA 
CCCAAAATGG AAATACGAGT AGTCACATTA GGATTAGATG GTGCTGGAAA AACTACTATT 
TTGTTCAAGT TAAAACAAGA TGAATTTATG CAGCCTATTC CAACAATTGG TTTTAATGTG 
GAAACTGTGG AATACAAAAA TCTAAAATTC ACCATTTGGG ATGTGGGTGA AAAACACAAA 
TTAAGACCAT TGTGGAAACA TTATTACCTC AATACACAAG CTGTTGTATT TGTTGTTGAC 
AGCAGTCACA GAGACAGAAT TAGTGAAGCA CACAGTGAAC TTGCGAAGTT GTTAACAGAA 
AAAGAACTTC GAGATGCCTT ACTCTTGATT TTTGCTAACA AACAGGATGT CGCTGGGGCA 
CTTTCGGTTG AAGAAATCAC CGAACTTCTC AGTCTCCATA AATTATGCTG CGGAAGGAGC 
TGGTATATTC AGGGCTGTGA TGCTCGAAGT GGTATGGGGC TCTACGAAGG GTTGGACTGG 
CTGTCCCGGC AACTTGTGGC TGCCGGAGTG TTGGATGTTG CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001094107.1
CDS	90..1814
Translation	MAALAVNKPGAGVDSGRQGSRGTAVVKVLECGVCEDVFSLQGDKVPRLLLCGHTVCHDCLSRLPLHGRAIRCPFDRQVTDLGDSGVWGLKKNFALLELLERLQNGHIGQYGAAEEALGISGESIIRCDEDEAHVASVYCTVCATHLCSECSQVTHSTKTLAKHRRVPLADKPHEKTMCCQHQVHAIEFVCLEEACQTSPLMCCVCKEYGKHQGHKHSVLEPEANQIRASILDMAHCIRTFTEEISDYSRKLVGIVQHIEGGEQIVEDGIGMAHTEHVPGTAENARSCVRAYFSDLHETLCRQEEMALSVVDAHVREKLIWLRQQQEDMTILLSQVSTACLHCEKTLQQDDCRVVLAKQEITRLLETLQKQQQQFTEVADHIQLDASIPVTFTKDNRVYIGPKMEIRVVTLGLDGAGKTTILFKLKQDEFMQPIPTIGFNVETVEYKNLKFTIWDVGEKHKLRPLWKHYYLNTQAVVFVVDSSHRDRISEAHSELAKLLTEKELRDALLLIFANKQDVAGALSVEEITELLSLHKLCCGRSWYIQGCDARSGMGLYEGLDWLSRQLVAAGVLDVA

Target ORF information:

RefSeq Version	NM_001100637.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tripartite motif-containing 23 (Trim23), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001100637.1

ORF Insert Sequence:

ATGGCTGCCC TCGCTGTAAA CAAGCCCGGA GCAGGAGTAG ACAGTGGGCG GCAGGGCAGC 
CGGGGGACGG CTGTGGTGAA GGTTCTGGAG TGTGGTGTTT GTGAAGATGT CTTCTCTCTG 
CAAGGAGATA AAGTTCCTCG CCTTCTCCTT TGTGGCCACA CAGTCTGTCA TGACTGTCTC 
TCTCGCTTGC CTCTGCATGG AAGAGCAATA CGTTGCCCAT TTGATCGACA AGTGACAGAC 
CTAGGAGATT CAGGTGTCTG GGGTTTGAAA AAAAATTTTG CTTTACTGGA GCTTTTAGAA 
CGTTTGCAAA ATGGACATAT TGGTCAGTAT GGAGCTGCAG AAGAAGCCCT TGGGATATCT 
GGAGAGAGTA TCATTCGCTG TGATGAAGAT GAAGCTCACG TTGCATCTGT GTACTGCACT 
GTGTGTGCGA CTCACTTGTG CTCAGAGTGT TCTCAAGTCA CTCATTCTAC AAAGACTCTG 
GCCAAGCATA GGCGAGTGCC TCTAGCTGAT AAACCTCATG AGAAGACCAT GTGCTGTCAG 
CACCAGGTAC ATGCCATTGA GTTTGTTTGC TTGGAAGAAG CTTGTCAAAC TAGTCCTCTC 
ATGTGCTGTG TCTGCAAAGA ATATGGAAAA CACCAGGGTC ACAAGCACTC AGTACTGGAG 
CCAGAAGCTA ACCAGATCCG AGCGTCAATT TTAGACATGG CTCACTGCAT ACGAACCTTC 
ACTGAGGAGA TCTCAGATTA TTCCAGAAAA TTAGTTGGGA TTGTTCAGCA CATTGAAGGA 
GGAGAACAAA TAGTGGAAGA TGGAATTGGA ATGGCTCACA CGGAACACGT CCCAGGTACT 
GCAGAGAATG CACGATCATG TGTCAGAGCT TATTTTTCTG ATTTGCATGA AACTCTTTGT 
CGTCAAGAAG AAATGGCTCT CAGTGTTGTT GATGCCCATG TTCGAGAAAA ACTGATTTGG 
CTTAGGCAGC AACAAGAGGA TATGACTATT CTCTTGTCCC AGGTTTCAAC AGCCTGTCTC 
CATTGTGAAA AGACTTTGCA GCAGGATGAT TGCAGAGTTG TCTTGGCAAA ACAAGAAATC 
ACAAGATTAT TAGAAACACT GCAGAAACAG CAGCAGCAGT TTACAGAAGT TGCAGATCAT 
ATTCAGTTGG ATGCCAGTAT TCCAGTCACT TTTACAAAGG ACAACAGAGT TTATATTGGA 
CCCAAAATGG AAATACGAGT AGTCACATTA GGATTAGATG GTGCTGGAAA AACTACTATT 
TTGTTCAAGT TAAAACAAGA TGAATTTATG CAGCCTATTC CAACAATTGG TTTTAATGTG 
GAAACTGTGG AATACAAAAA TCTAAAATTC ACCATTTGGG ATGTGGGTGA AAAACACAAA 
TTAAGACCAT TGTGGAAACA TTATTACCTC AATACACAAG CTGTTGTATT TGTTGTTGAC 
AGCAGTCACA GAGACAGAAT TAGTGAAGCA CACAGTGAAC TTGCGAAGTT GTTAACAGAA 
AAAGAACTTC GAGATGCCTT ACTCTTGATT TTTGCTAACA AACAGGATGT CGCTGGGGCA 
CTTTCGGTTG AAGAAATCAC CGAACTTCTC AGTCTCCATA AATTATGCTG CGGAAGGAGC 
TGGTATATTC AGGGCTGTGA TGCTCGAAGT GGTATGGGGC TCTACGAAGG GTTGGACTGG 
CTGTCCCGGC AACTTGTGGC TGCCGGAGTG TTGGATGTTG CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trim23 ( XM_008760683.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008758905.1 Rattus norvegicus tripartite motif-containing 23 (Trim23), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa43697
Clone ID Related Accession (Same CDS sequence)	XM_008760683.2
Accession Version	XM_008760683.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1806bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase TRIM23 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008760683.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCCC TCGCTGTAAA CAAGCCCGGA GCAGGAGTAG ACAGTGGGCG GCAGGGCAGC 
CGGGGGACGG CTGTGGTGAA GACTATACAT GAAATATTAG CCCATGGACC TGAGTGTCAG 
AGTGAGCCAG TTCTCGCAGC TTTTCTTCCT CACTATCTTT CAGTTCTGGA GTGTGGTGTT 
TGTGAAGATG TCTTCTCTCT GCAAGGAGAT AAAGTTCCTC GCCTTCTCCT TTGTGGCCAC 
ACAGTCTGTC ATGACTGTCT CACACGCTTG CCTCTGCATG GAAGAGCAAT ACGTTGCCCA 
TTTGATCGAC AAGTGACAGA CCTAGGAGAT TCAGGTGTCT GGGGTTTGAA AAAAAATTTT 
GCTTTACTGG AGCTTTTAGA ACGTTTGCAA AATGGACATA TTGGTCAGTA TGGAGCTGCA 
GAAGAAGCCC TTGGGATATC TGGAGAGAGT ATCATTCGCT GTGATGAAGA TGAAGCTCAC 
GTTGCATCTG TGTACTGCAC TGTGTGTGCG ACTCACTTGT GCTCAGAGTG TTCTCAAGTC 
ACACATTCTA CAAAGACACT GGCCAAGCAT AGGCGAGTGC CTCTAGCTGA TAAACCTCAT 
GAGAAGACCA TGTGCTGTCA GCACCAGGTA CATGCCATTG AGTTTGTTTG CTTGGAAGAA 
GCTTGTCAAA CTAGTCCTCT CATGTGCTGT GTCTGCAAAG AATATGGAAA ACACCAGGGT 
CACAAGCACT CAGTACTGGA GCCAGAAGCT AACCAGATCC GAGCGTCAAT TTTAGACATG 
GCTCACTGCA TACGAACCTT CACTGAGGAG ATCTCAGATT ATTCCAGAAA ATTAGTTGGG 
ATTGTTCAGC ACATTGAAGG AGGAGAACAA ATAGTGGAAG ATGGAATTGG AATGGCTCAC 
ACGGAACACG TCCCAGGTAC TGCAGAGAAT GCACGATCAT GTGTCAGAGC TTATTTTTCT 
GATTTGCATG AAACTCTTTG TCGTCAAGAA GAAATGGCTC TCAGTGTTGT TGATGCCCAT 
GTTCGAGAAA AACTGATTTG GCTTAGGCAG CAACAAGAGG ATATGACTAT TCTCTTGTCC 
CAGGTTTCAA CAGCCTGTCT CCATTGTGAA AAGACTTTGC AGCAGGATGA TTGCAGAGTT 
GTCTTGGCAA AACAAGAAAT CACAAGATTA TTAGAAACAC TGCAGAAACA GCAGCAGCAG 
TTTACAGAAG TTGCAGATCA TATTCAGTTG GATGCCAGTA TTCCAGTCAC TTTTACAAAG 
GACAACAGAG TTCATATTGG ACCCAAAATG GAAATCCGAG TAGTCACATT AGGATTAGAT 
GGTGCTGGAA AAACTACTAT TTTGTTCAAG TTAAAACAAG ATGAATTTAT GCAGCCTATT 
CCAACAATTG GTTTTAATGT GGAAACTGTG GAATACAAAA ATCTAAAATT CACCATTTGG 
GATGTGGGTG GAAAACACAA ATTAAGACCA TTGTGGAAAC ATTATTACCT CAATACACAA 
GCTGTTGTAT TTGTTGTTGA CAGCAGTCAC AGAGACAGAA TTAGTGAAGC ACACAGTGAA 
CTTGCGAAGT TGTTAACAGA AAAAGAACTT CGAGATGCCT TACTCTTGAT TTTTGCTAAC 
AAACAGGATG TCGCTGGGGC ACTTTCGGTT GAAGAAATCA CCGAACTTCT CAGTCTCCAT 
AAATTATGCT GCGGAAGGAG CTGGTATATT CAGGGCTGTG ATGCTCGAAG TGGTATGGGG 
CTCTACGAAG GGTTGGACTG GCTGTCCCGG CAACTTGTGG CTGCCGGAGT GTTGGATGTT 
GCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008758905.1
CDS	94..1899
Translation	MAALAVNKPGAGVDSGRQGSRGTAVVKTIHEILAHGPECQSEPVLAAFLPHYLSVLECGVCEDVFSLQGDKVPRLLLCGHTVCHDCLTRLPLHGRAIRCPFDRQVTDLGDSGVWGLKKNFALLELLERLQNGHIGQYGAAEEALGISGESIIRCDEDEAHVASVYCTVCATHLCSECSQVTHSTKTLAKHRRVPLADKPHEKTMCCQHQVHAIEFVCLEEACQTSPLMCCVCKEYGKHQGHKHSVLEPEANQIRASILDMAHCIRTFTEEISDYSRKLVGIVQHIEGGEQIVEDGIGMAHTEHVPGTAENARSCVRAYFSDLHETLCRQEEMALSVVDAHVREKLIWLRQQQEDMTILLSQVSTACLHCEKTLQQDDCRVVLAKQEITRLLETLQKQQQQFTEVADHIQLDASIPVTFTKDNRVHIGPKMEIRVVTLGLDGAGKTTILFKLKQDEFMQPIPTIGFNVETVEYKNLKFTIWDVGGKHKLRPLWKHYYLNTQAVVFVVDSSHRDRISEAHSELAKLLTEKELRDALLLIFANKQDVAGALSVEEITELLSLHKLCCGRSWYIQGCDARSGMGLYEGLDWLSRQLVAAGVLDVA

Target ORF information:

RefSeq Version	XM_008760683.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tripartite motif-containing 23 (Trim23), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008760683.2

ORF Insert Sequence:

ATGGCTGCCC TCGCTGTAAA CAAGCCCGGA GCAGGAGTAG ACAGTGGGCG GCAGGGCAGC 
CGGGGGACGG CTGTGGTGAA GACTATACAT GAAATATTAG CCCATGGACC TGAGTGTCAG 
AGTGAGCCAG TTCTCGCAGC TTTTCTTCCT CACTATCTTT CAGTTCTGGA GTGTGGTGTT 
TGTGAAGATG TCTTCTCTCT GCAAGGAGAT AAAGTTCCTC GCCTTCTCCT TTGTGGCCAC 
ACAGTCTGTC ATGACTGTCT CACACGCTTG CCTCTGCATG GAAGAGCAAT ACGTTGCCCA 
TTTGATCGAC AAGTGACAGA CCTAGGAGAT TCAGGTGTCT GGGGTTTGAA AAAAAATTTT 
GCTTTACTGG AGCTTTTAGA ACGTTTGCAA AATGGACATA TTGGTCAGTA TGGAGCTGCA 
GAAGAAGCCC TTGGGATATC TGGAGAGAGT ATCATTCGCT GTGATGAAGA TGAAGCTCAC 
GTTGCATCTG TGTACTGCAC TGTGTGTGCG ACTCACTTGT GCTCAGAGTG TTCTCAAGTC 
ACACATTCTA CAAAGACACT GGCCAAGCAT AGGCGAGTGC CTCTAGCTGA TAAACCTCAT 
GAGAAGACCA TGTGCTGTCA GCACCAGGTA CATGCCATTG AGTTTGTTTG CTTGGAAGAA 
GCTTGTCAAA CTAGTCCTCT CATGTGCTGT GTCTGCAAAG AATATGGAAA ACACCAGGGT 
CACAAGCACT CAGTACTGGA GCCAGAAGCT AACCAGATCC GAGCGTCAAT TTTAGACATG 
GCTCACTGCA TACGAACCTT CACTGAGGAG ATCTCAGATT ATTCCAGAAA ATTAGTTGGG 
ATTGTTCAGC ACATTGAAGG AGGAGAACAA ATAGTGGAAG ATGGAATTGG AATGGCTCAC 
ACGGAACACG TCCCAGGTAC TGCAGAGAAT GCACGATCAT GTGTCAGAGC TTATTTTTCT 
GATTTGCATG AAACTCTTTG TCGTCAAGAA GAAATGGCTC TCAGTGTTGT TGATGCCCAT 
GTTCGAGAAA AACTGATTTG GCTTAGGCAG CAACAAGAGG ATATGACTAT TCTCTTGTCC 
CAGGTTTCAA CAGCCTGTCT CCATTGTGAA AAGACTTTGC AGCAGGATGA TTGCAGAGTT 
GTCTTGGCAA AACAAGAAAT CACAAGATTA TTAGAAACAC TGCAGAAACA GCAGCAGCAG 
TTTACAGAAG TTGCAGATCA TATTCAGTTG GATGCCAGTA TTCCAGTCAC TTTTACAAAG 
GACAACAGAG TTCATATTGG ACCCAAAATG GAAATCCGAG TAGTCACATT AGGATTAGAT 
GGTGCTGGAA AAACTACTAT TTTGTTCAAG TTAAAACAAG ATGAATTTAT GCAGCCTATT 
CCAACAATTG GTTTTAATGT GGAAACTGTG GAATACAAAA ATCTAAAATT CACCATTTGG 
GATGTGGGTG GAAAACACAA ATTAAGACCA TTGTGGAAAC ATTATTACCT CAATACACAA 
GCTGTTGTAT TTGTTGTTGA CAGCAGTCAC AGAGACAGAA TTAGTGAAGC ACACAGTGAA 
CTTGCGAAGT TGTTAACAGA AAAAGAACTT CGAGATGCCT TACTCTTGAT TTTTGCTAAC 
AAACAGGATG TCGCTGGGGC ACTTTCGGTT GAAGAAATCA CCGAACTTCT CAGTCTCCAT 
AAATTATGCT GCGGAAGGAG CTGGTATATT CAGGGCTGTG ATGCTCGAAG TGGTATGGGG 
CTCTACGAAG GGTTGGACTG GCTGTCCCGG CAACTTGTGG CTGCCGGAGT GTTGGATGTT 
GCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trim23 ( XM_008760684.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008758906.1 Rattus norvegicus tripartite motif-containing 23 (Trim23), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa43698
Clone ID Related Accession (Same CDS sequence)	XM_008760684.2
Accession Version	XM_008760684.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1707bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase TRIM23 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008760684.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCCC TCGCTGTAAA CAAGCCCGGA GCAGGAGTAG ACAGTGGGCG GCAGGGCAGC 
CGGGGGACGG CTGTGGTGAA GACTATACAT GAAATATTAG CCCATGGACC TGAGTGTCAG 
AGTGAGCCAG TTCTCGCAGC TTTTCTTCCT CACTATCTTT CAGTTCTGGA GTGTGGTGTT 
TGTGAAGATG TCTTCTCTCT GCAAGGAGAT AAAGTTCCTC GCCTTCTCCT TTGTGGCCAC 
ACAGTCTGTC ATGACTGTCT CACACGCTTG CCTCTGCATG GAAGAGCAAT ACGTTGCCCA 
TTTGATCGAC AAGTGACAGA CCTAGGAGAT TCAGGTGTCT GGGGTTTGAA AAAAAATTTT 
GCTTTACTGG AGCTTTTAGA ACGTTTGCAA AATGGACATA TTGGTCAGTA TGGAGCTGCA 
GAAGAAGCCC TTGGGATATC TGGAGAGAGT ATCATTCGCT GTGATGAAGA TGAAGCTCAC 
GTTGCATCTG TGTACTGCAC TGTGTGTGCG ACTCACTTGT GCTCAGAGTG TTCTCAAGTC 
ACACATTCTA CAAAGACACT GGCCAAGCAT AGGCGAGTGC CTCTAGCTGA TAAACCTCAT 
GAGAAGACCA TGTGCTGTCA GCACCAGCAC TCAGTACTGG AGCCAGAAGC TAACCAGATC 
CGAGCGTCAA TTTTAGACAT GGCTCACTGC ATACGAACCT TCACTGAGGA GATCTCAGAT 
TATTCCAGAA AATTAGTTGG GATTGTTCAG CACATTGAAG GAGGAGAACA AATAGTGGAA 
GATGGAATTG GAATGGCTCA CACGGAACAC GTCCCAGGTA CTGCAGAGAA TGCACGATCA 
TGTGTCAGAG CTTATTTTTC TGATTTGCAT GAAACTCTTT GTCGTCAAGA AGAAATGGCT 
CTCAGTGTTG TTGATGCCCA TGTTCGAGAA AAACTGATTT GGCTTAGGCA GCAACAAGAG 
GATATGACTA TTCTCTTGTC CCAGGTTTCA ACAGCCTGTC TCCATTGTGA AAAGACTTTG 
CAGCAGGATG ATTGCAGAGT TGTCTTGGCA AAACAAGAAA TCACAAGATT ATTAGAAACA 
CTGCAGAAAC AGCAGCAGCA GTTTACAGAA GTTGCAGATC ATATTCAGTT GGATGCCAGT 
ATTCCAGTCA CTTTTACAAA GGACAACAGA GTTCATATTG GACCCAAAAT GGAAATCCGA 
GTAGTCACAT TAGGATTAGA TGGTGCTGGA AAAACTACTA TTTTGTTCAA GTTAAAACAA 
GATGAATTTA TGCAGCCTAT TCCAACAATT GGTTTTAATG TGGAAACTGT GGAATACAAA 
AATCTAAAAT TCACCATTTG GGATGTGGGT GGAAAACACA AATTAAGACC ATTGTGGAAA 
CATTATTACC TCAATACACA AGCTGTTGTA TTTGTTGTTG ACAGCAGTCA CAGAGACAGA 
ATTAGTGAAG CACACAGTGA ACTTGCGAAG TTGTTAACAG AAAAAGAACT TCGAGATGCC 
TTACTCTTGA TTTTTGCTAA CAAACAGGAT GTCGCTGGGG CACTTTCGGT TGAAGAAATC 
ACCGAACTTC TCAGTCTCCA TAAATTATGC TGCGGAAGGA GCTGGTATAT TCAGGGCTGT 
GATGCTCGAA GTGGTATGGG GCTCTACGAA GGGTTGGACT GGCTGTCCCG GCAACTTGTG 
GCTGCCGGAG TGTTGGATGT TGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008758906.1
CDS	95..1801
Translation	MAALAVNKPGAGVDSGRQGSRGTAVVKTIHEILAHGPECQSEPVLAAFLPHYLSVLECGVCEDVFSLQGDKVPRLLLCGHTVCHDCLTRLPLHGRAIRCPFDRQVTDLGDSGVWGLKKNFALLELLERLQNGHIGQYGAAEEALGISGESIIRCDEDEAHVASVYCTVCATHLCSECSQVTHSTKTLAKHRRVPLADKPHEKTMCCQHQHSVLEPEANQIRASILDMAHCIRTFTEEISDYSRKLVGIVQHIEGGEQIVEDGIGMAHTEHVPGTAENARSCVRAYFSDLHETLCRQEEMALSVVDAHVREKLIWLRQQQEDMTILLSQVSTACLHCEKTLQQDDCRVVLAKQEITRLLETLQKQQQQFTEVADHIQLDASIPVTFTKDNRVHIGPKMEIRVVTLGLDGAGKTTILFKLKQDEFMQPIPTIGFNVETVEYKNLKFTIWDVGGKHKLRPLWKHYYLNTQAVVFVVDSSHRDRISEAHSELAKLLTEKELRDALLLIFANKQDVAGALSVEEITELLSLHKLCCGRSWYIQGCDARSGMGLYEGLDWLSRQLVAAGVLDVA

Target ORF information:

RefSeq Version	XM_008760684.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tripartite motif-containing 23 (Trim23), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008760684.2

ORF Insert Sequence:

ATGGCTGCCC TCGCTGTAAA CAAGCCCGGA GCAGGAGTAG ACAGTGGGCG GCAGGGCAGC 
CGGGGGACGG CTGTGGTGAA GACTATACAT GAAATATTAG CCCATGGACC TGAGTGTCAG 
AGTGAGCCAG TTCTCGCAGC TTTTCTTCCT CACTATCTTT CAGTTCTGGA GTGTGGTGTT 
TGTGAAGATG TCTTCTCTCT GCAAGGAGAT AAAGTTCCTC GCCTTCTCCT TTGTGGCCAC 
ACAGTCTGTC ATGACTGTCT CACACGCTTG CCTCTGCATG GAAGAGCAAT ACGTTGCCCA 
TTTGATCGAC AAGTGACAGA CCTAGGAGAT TCAGGTGTCT GGGGTTTGAA AAAAAATTTT 
GCTTTACTGG AGCTTTTAGA ACGTTTGCAA AATGGACATA TTGGTCAGTA TGGAGCTGCA 
GAAGAAGCCC TTGGGATATC TGGAGAGAGT ATCATTCGCT GTGATGAAGA TGAAGCTCAC 
GTTGCATCTG TGTACTGCAC TGTGTGTGCG ACTCACTTGT GCTCAGAGTG TTCTCAAGTC 
ACACATTCTA CAAAGACACT GGCCAAGCAT AGGCGAGTGC CTCTAGCTGA TAAACCTCAT 
GAGAAGACCA TGTGCTGTCA GCACCAGCAC TCAGTACTGG AGCCAGAAGC TAACCAGATC 
CGAGCGTCAA TTTTAGACAT GGCTCACTGC ATACGAACCT TCACTGAGGA GATCTCAGAT 
TATTCCAGAA AATTAGTTGG GATTGTTCAG CACATTGAAG GAGGAGAACA AATAGTGGAA 
GATGGAATTG GAATGGCTCA CACGGAACAC GTCCCAGGTA CTGCAGAGAA TGCACGATCA 
TGTGTCAGAG CTTATTTTTC TGATTTGCAT GAAACTCTTT GTCGTCAAGA AGAAATGGCT 
CTCAGTGTTG TTGATGCCCA TGTTCGAGAA AAACTGATTT GGCTTAGGCA GCAACAAGAG 
GATATGACTA TTCTCTTGTC CCAGGTTTCA ACAGCCTGTC TCCATTGTGA AAAGACTTTG 
CAGCAGGATG ATTGCAGAGT TGTCTTGGCA AAACAAGAAA TCACAAGATT ATTAGAAACA 
CTGCAGAAAC AGCAGCAGCA GTTTACAGAA GTTGCAGATC ATATTCAGTT GGATGCCAGT 
ATTCCAGTCA CTTTTACAAA GGACAACAGA GTTCATATTG GACCCAAAAT GGAAATCCGA 
GTAGTCACAT TAGGATTAGA TGGTGCTGGA AAAACTACTA TTTTGTTCAA GTTAAAACAA 
GATGAATTTA TGCAGCCTAT TCCAACAATT GGTTTTAATG TGGAAACTGT GGAATACAAA 
AATCTAAAAT TCACCATTTG GGATGTGGGT GGAAAACACA AATTAAGACC ATTGTGGAAA 
CATTATTACC TCAATACACA AGCTGTTGTA TTTGTTGTTG ACAGCAGTCA CAGAGACAGA 
ATTAGTGAAG CACACAGTGA ACTTGCGAAG TTGTTAACAG AAAAAGAACT TCGAGATGCC 
TTACTCTTGA TTTTTGCTAA CAAACAGGAT GTCGCTGGGG CACTTTCGGT TGAAGAAATC 
ACCGAACTTC TCAGTCTCCA TAAATTATGC TGCGGAAGGA GCTGGTATAT TCAGGGCTGT 
GATGCTCGAA GTGGTATGGG GCTCTACGAA GGGTTGGACT GGCTGTCCCG GCAACTTGTG 
GCTGCCGGAG TGTTGGATGT TGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Systematic analysis of dimeric E3-RING interactions reveals increased combinatorial complexity in human ubiquitination networks.
Molecular & cellular proteomics : MCP11(7)M111.016162(2012 Jul)
Woodsmith J,Jenn RC,Sanderson CM

E3 ubiquitin ligase activity of the trifunctional ARD1 (ADP-ribosylation factor domain protein 1).
Proceedings of the National Academy of Sciences of the United States of America102(6)1945-50(2005 Feb)
Vichi A,Payne DM,Pacheco-Rodriguez G,Moss J,Vaughan M

The tripartite motif family identifies cell compartments.
The EMBO journal20(9)2140-51(2001 May)
Reymond A,Meroni G,Fantozzi A,Merla G,Cairo S,Luzi L,Riganelli D,Zanaria E,Messali S,Cainarca S,Guffanti A,Minucci S,Pelicci PG,Ballabio A

Localization of ADP-ribosylation factor domain protein 1 (ARD1) in lysosomes and Golgi apparatus.
Proceedings of the National Academy of Sciences of the United States of America95(15)8613-8(1998 Jul)
Vitale N,Horiba K,Ferrans VJ,Moss J,Vaughan M

ARD1, a 64-kDa bifunctional protein containing an 18-kDa GTP-binding ADP-ribosylation factor domain and a 46-kDa GTPase-activating domain.
Proceedings of the National Academy of Sciences of the United States of America93(5)1941-4(1996 Mar)
Vitale N,Moss J,Vaughan M

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Trim23 cDNA ORF clone, Rattus norvegicus(Norway rat)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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