Sec61a1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sec61a1
Entrez Gene ID	80843
Full Name	Sec61 translocon alpha 1 subunit
Synonyms	Sec61a
General protein information	Preferred Names Sec61 translocon alpha 1 subunit Names protein transport protein Sec61 subunit alpha isoform 1 SEC61, alpha subunit Sec61 alpha 1 subunit Sec61 alpha subunit homolog protein transport protein Sec61 subunit alpha sec61 alpha-1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 4 Map Location: 4q34
Summary	involved in protein translocation across the endoplasmic reticulum membrane [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_199256.2	NP_954865.1	protein transport protein Sec61 subunit alpha isoform 1

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0E01145g	XP_454000.1
Pan troglodytes (chimpanzee)	SEC61A1	XP_003310030.1
Danio rerio (zebrafish)	sec61a1	NP_705945.1
Danio rerio (zebrafish)	sec61al2	NP_963871.1
Oryza sativa (rice)	Os09g0347700	NP_001062945.1
Eremothecium gossypii	AGOS_AFR596W	NP_986143.1
Bos taurus (cattle)	SEC61A1	NP_001035594.1
Gallus gallus (chicken)	SEC61A1	XP_414364.3
Saccharomyces cerevisiae (baker's yeast)	SEC61	NP_013482.1
Schizosaccharomyces pombe (fission yeast)	sec61	NP_595226.1
Homo sapiens (human)	SEC61A1	NP_037468.1
Rattus norvegicus (Norway rat)	Sec61a1	NP_954865.1
Arabidopsis thaliana (thale cress)	AT1G29310	NP_174225.2
Xenopus tropicalis (tropical clawed frog)	sec61a1	NP_001004801.1
Oryza sativa (rice)	Os03g0213100	NP_001049359.1
Neurospora crassa	NCU08897	XP_958835.1
Canis lupus familiaris (dog)	SEC61A1	NP_001003315.1
Mus musculus (house mouse)	Sec61a1	NP_058602.1
Arabidopsis thaliana (thale cress)	AT2G34250	NP_180972.1
Magnaporthe oryzae (rice blast fungus)	MGG_04856	XP_003712335.1
Macaca mulatta (Rhesus monkey)	SEC61A1	XP_002798757.1

Gene Ontology
Bibliography

Related articles in PubMed

Chaperone-Mediated Sec61 Channel Gating during ER Import of Small Precursor Proteins Overcomes Sec61 Inhibitor-Reinforced Energy Barrier.
Haßdenteufel S, Johnson N, Paton AW, Paton JC, High S, Zimmermann R
Cell reports23(5)1373-1386(2018 05)

Plasma cell deficiency in human subjects with heterozygous mutations in Sec61 translocon alpha 1 subunit (SEC61A1).
Schubert D, Klein MC, Hassdenteufel S, Caballero-Oteyza A, Yang L, Proietti M, Bulashevska A, Kemming J, Kühn J, Winzer S, Rusch S, Fliegauf M, Schäffer AA, Pfeffer S, Geiger R, Cavalié A, Cao H, Yang F, Li Y, Rizzi M, Eibel H, Kobbe R, Marks AL, Peppers BP, Hostoffer RW, Puck JM, Zimmermann R, Grimbacher B
The Journal of allergy and clinical immunology141(4)1427-1438(2018 04)

Heterozygous Loss-of-Function SEC61A1 Mutations Cause Autosomal-Dominant Tubulo-Interstitial and Glomerulocystic Kidney Disease with Anemia.
Bolar NA, Golzio C, Živná M, Hayot G, Van Hemelrijk C, Schepers D, Vandeweyer G, Hoischen A, Huyghe JR, Raes A, Matthys E, Sys E, Azou M, Gubler MC, Praet M, Van Camp G, McFadden K, Pediaditakis I, Přistoupilová A, Hodaňová K, Vyleťal P, Hartmannová H, Stránecký V, Hůlková H, Barešová V, Jedličková I, Sovová J, Hnízda A, Kidd K, Bleyer AJ, Spong RS, Vande Walle J, Mortier G, Brunner H, Van Laer L, Kmoch S, Katsanis N, Loeys BL
American journal of human genetics99(1)174-87(2016 Jul)

Different effects of Sec61α, Sec62 and Sec63 depletion on transport of polypeptides into the endoplasmic reticulum of mammalian cells.
Lang S, Benedix J, Fedeles SV, Schorr S, Schirra C, Schäuble N, Jalal C, Greiner M, Hassdenteufel S, Tatzelt J, Kreutzer B, Edelmann L, Krause E, Rettig J, Somlo S, Zimmermann R, Dudek J
Journal of cell science125(Pt 8)1958-69(2012 Apr)

Control of translocation through the Sec61 translocon by nascent polypeptide structure within the ribosome.
Daniel CJ, Conti B, Johnson AE, Skach WR
The Journal of biological chemistry283(30)20864-73(2008 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Sec61a1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sec61a1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa04663	NM_199256.2 Latest version!	Rattus norvegicus SEC61 translocon subunit alpha 1 (Sec61a1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sec61a1 ( NM_199256.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_954865.1 Rattus norvegicus SEC61 translocon subunit alpha 1 (Sec61a1), mRNA.

Price & Availability↑

CloneID	ORa04663
Clone ID Related Accession (Same CDS sequence)	NM_199256.2
Accession Version	NM_199256.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1431bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575907200000
Organism	Rattus norvegicus(Norway rat)
Product	protein transport protein Sec61 subunit alpha isoform 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC094530.1. On May 15, 2005 this sequence version replaced NM_199256.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC094530.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCGATCA AATTTCTGGA AGTCATCAAG CCATTCTGTG TCATCTTGCC GGAAATTCAG 
AAGCCAGAGA GAAAGATTCA GTTTAAGGAG AAGGTGTTGT GGACGGCCAT CACCCTCTTC 
ATCTTCTTAG TATGCTGCCA GATTCCCCTG TTTGGCATCA TGTCTTCAGA CTCAGCTGAC 
CCCTTCTACT GGATGCGAGT GATTCTGGCC TCTAACAGAG GTACCTTGAT GGAGCTGGGT 
ATCTCTCCTA TTGTCACGTC CGGCCTTATC ATGCAGCTCT TAGCTGGTGC CAAGATAATT 
GAAGTTGGGG ACACCCCGAA AGACCGAGCT CTCTTCAATG GAGCCCAAAA GTTGTTTGGA 
ATGATCATCA CTATCGGTCA GTCCATTGTG TACGTGATGA CGGGCATGTA CGGGGACCCT 
TCAGAGATGG GTGCTGGAAT CTGCCTGCTG ATCACCATTC AGCTCTTTGT TGCTGGCTTA 
ATTGTCCTAC TTTTGGATGA ACTCCTGCAG AAAGGATATG GCTTGGGCTC TGGCATCTCT 
CTCTTCATTG CGACTAATAT CTGTGAGACC ATTGTATGGA AGGCATTCAG CCCCACCACT 
GTCAACACTG GCCGAGGGAT GGAATTTGAA GGCGCCATCA TTGCGCTGTT CCACCTGCTG 
GCCACCCGCA CAGACAAGGT CCGAGCCCTT CGAGAGGCAT TCTACCGCCA GAACCTCCCT 
AACCTCATGA ACCTGATTGC CACCATCTTC GTCTTTGCTG TTGTCATCTA TTTCCAGGGC 
TTCCGAGTGG ACCTTCCAAT CAAGTCGGCG CGTTACCGAG GCCAGTACAA TACCTACCCC 
ATCAAGCTCT TCTACACCTC CAACATTCCC ATCATCTTGC AGTCTGCTCT GGTGTCCAAC 
CTGTATGTCA TCTCCCAGAT GCTGTCAGCA CGTTTCAGTG GCAACCTGCT GGTTAGCCTG 
CTGGGCACCT GGTCGGATAC GTCATCTGGG GGCCCAGCCC GTGCTTACCC AGTTGGTGGC 
CTGTGTTACT ACCTGTCTCC TCCGGAATCA TTCGGCTCAG TGCTGGAGGA CCCTGTCCAT 
GCGGTCGTGT ACATTGTATT CATGCTGGGT TCCTGTGCAT TCTTTTCCAA AACATGGATC 
GAAGTTTCGG GTTCTTCTGC CAAAGATGTT GCCAAGCAGC TGAAGGAGCA GCAGATGGTG 
ATGAGAGGCC ACAGGGAGAC CTCCATGGTG CATGAGCTCA ACCGGTACAT CCCCACAGCT 
GCAGCCTTCG GTGGGCTGTG CATTGGGGCC CTCTCTGTCC TGGCTGATTT CCTGGGTGCT 
ATCGGGTCTG GCACCGGGAT CCTGCTGGCG GTCACCATCA TCTACCAGTA CTTTGAGATC 
TTCGTCAAAG AGCAGAGCGA GGTGGGCAGC ATGGGAGCCC TTCTCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_954865.1
CDS	108..1538
Translation	MAIKFLEVIKPFCVILPEIQKPERKIQFKEKVLWTAITLFIFLVCCQIPLFGIMSSDSADPFYWMRVILASNRGTLMELGISPIVTSGLIMQLLAGAKIIEVGDTPKDRALFNGAQKLFGMIITIGQSIVYVMTGMYGDPSEMGAGICLLITIQLFVAGLIVLLLDELLQKGYGLGSGISLFIATNICETIVWKAFSPTTVNTGRGMEFEGAIIALFHLLATRTDKVRALREAFYRQNLPNLMNLIATIFVFAVVIYFQGFRVDLPIKSARYRGQYNTYPIKLFYTSNIPIILQSALVSNLYVISQMLSARFSGNLLVSLLGTWSDTSSGGPARAYPVGGLCYYLSPPESFGSVLEDPVHAVVYIVFMLGSCAFFSKTWIEVSGSSAKDVAKQLKEQQMVMRGHRETSMVHELNRYIPTAAAFGGLCIGALSVLADFLGAIGSGTGILLAVTIIYQYFEIFVKEQSEVGSMGALLF

Target ORF information:

RefSeq Version	NM_199256.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus SEC61 translocon subunit alpha 1 (Sec61a1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199256.2

ORF Insert Sequence:

ATGGCGATCA AATTTCTGGA AGTCATCAAG CCATTCTGTG TCATCTTGCC GGAAATTCAG 
AAGCCAGAGA GAAAGATTCA GTTTAAGGAG AAGGTGTTGT GGACGGCCAT CACCCTCTTC 
ATCTTCTTAG TATGCTGCCA GATTCCCCTG TTTGGCATCA TGTCTTCAGA CTCAGCTGAC 
CCCTTCTACT GGATGCGAGT GATTCTGGCC TCTAACAGAG GTACCTTGAT GGAGCTGGGT 
ATCTCTCCTA TTGTCACGTC CGGCCTTATC ATGCAGCTCT TAGCTGGTGC CAAGATAATT 
GAAGTTGGGG ACACCCCGAA AGACCGAGCT CTCTTCAATG GAGCCCAAAA GTTGTTTGGA 
ATGATCATCA CTATCGGTCA GTCCATTGTG TACGTGATGA CGGGCATGTA CGGGGACCCT 
TCAGAGATGG GTGCTGGAAT CTGCCTGCTG ATCACCATTC AGCTCTTTGT TGCTGGCTTA 
ATTGTCCTAC TTTTGGATGA ACTCCTGCAG AAAGGATATG GCTTGGGCTC TGGCATCTCT 
CTCTTCATTG CGACTAATAT CTGTGAGACC ATTGTATGGA AGGCATTCAG CCCCACCACT 
GTCAACACTG GCCGAGGGAT GGAATTTGAA GGCGCCATCA TTGCGCTGTT CCACCTGCTG 
GCCACCCGCA CAGACAAGGT CCGAGCCCTT CGAGAGGCAT TCTACCGCCA GAACCTCCCT 
AACCTCATGA ACCTGATTGC CACCATCTTC GTCTTTGCTG TTGTCATCTA TTTCCAGGGC 
TTCCGAGTGG ACCTTCCAAT CAAGTCGGCG CGTTACCGAG GCCAGTACAA TACCTACCCC 
ATCAAGCTCT TCTACACCTC CAACATTCCC ATCATCTTGC AGTCTGCTCT GGTGTCCAAC 
CTGTATGTCA TCTCCCAGAT GCTGTCAGCA CGTTTCAGTG GCAACCTGCT GGTTAGCCTG 
CTGGGCACCT GGTCGGATAC GTCATCTGGG GGCCCAGCCC GTGCTTACCC AGTTGGTGGC 
CTGTGTTACT ACCTGTCTCC TCCGGAATCA TTCGGCTCAG TGCTGGAGGA CCCTGTCCAT 
GCGGTCGTGT ACATTGTATT CATGCTGGGT TCCTGTGCAT TCTTTTCCAA AACATGGATC 
GAAGTTTCGG GTTCTTCTGC CAAAGATGTT GCCAAGCAGC TGAAGGAGCA GCAGATGGTG 
ATGAGAGGCC ACAGGGAGAC CTCCATGGTG CATGAGCTCA ACCGGTACAT CCCCACAGCT 
GCAGCCTTCG GTGGGCTGTG CATTGGGGCC CTCTCTGTCC TGGCTGATTT CCTGGGTGCT 
ATCGGGTCTG GCACCGGGAT CCTGCTGGCG GTCACCATCA TCTACCAGTA CTTTGAGATC 
TTCGTCAAAG AGCAGAGCGA GGTGGGCAGC ATGGGAGCCC TTCTCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Chaperone-Mediated Sec61 Channel Gating during ER Import of Small Precursor Proteins Overcomes Sec61 Inhibitor-Reinforced Energy Barrier.
Cell reports23(5)1373-1386(2018 05)
Haßdenteufel S,Johnson N,Paton AW,Paton JC,High S,Zimmermann R

Plasma cell deficiency in human subjects with heterozygous mutations in Sec61 translocon alpha 1 subunit (SEC61A1).
The Journal of allergy and clinical immunology141(4)1427-1438(2018 04)
Schubert D,Klein MC,Hassdenteufel S,Caballero-Oteyza A,Yang L,Proietti M,Bulashevska A,Kemming J,Kühn J,Winzer S,Rusch S,Fliegauf M,Schäffer AA,Pfeffer S,Geiger R,Cavalié A,Cao H,Yang F,Li Y,Rizzi M,Eibel H,Kobbe R,Marks AL,Peppers BP,Hostoffer RW,Puck JM,Zimmermann R,Grimbacher B

Heterozygous Loss-of-Function SEC61A1 Mutations Cause Autosomal-Dominant Tubulo-Interstitial and Glomerulocystic Kidney Disease with Anemia.
American journal of human genetics99(1)174-87(2016 Jul)
Bolar NA,Golzio C,Živná M,Hayot G,Van Hemelrijk C,Schepers D,Vandeweyer G,Hoischen A,Huyghe JR,Raes A,Matthys E,Sys E,Azou M,Gubler MC,Praet M,Van Camp G,McFadden K,Pediaditakis I,Přistoupilová A,Hodaňová K,Vyleťal P,Hartmannová H,Stránecký V,Hůlková H,Barešová V,Jedličková I,Sovová J,Hnízda A,Kidd K,Bleyer AJ,Spong RS,Vande Walle J,Mortier G,Brunner H,Van Laer L,Kmoch S,Katsanis N,Loeys BL

Different effects of Sec61α, Sec62 and Sec63 depletion on transport of polypeptides into the endoplasmic reticulum of mammalian cells.
Journal of cell science125(Pt 8)1958-69(2012 Apr)
Lang S,Benedix J,Fedeles SV,Schorr S,Schirra C,Schäuble N,Jalal C,Greiner M,Hassdenteufel S,Tatzelt J,Kreutzer B,Edelmann L,Krause E,Rettig J,Somlo S,Zimmermann R,Dudek J

Control of translocation through the Sec61 translocon by nascent polypeptide structure within the ribosome.
The Journal of biological chemistry283(30)20864-73(2008 Jul)
Daniel CJ,Conti B,Johnson AE,Skach WR

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Sec61a1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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