Grid2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Grid2
Entrez Gene ID	79220
Full Name	glutamate ionotropic receptor delta type subunit 2
Synonyms	GluD2
General protein information	Preferred Names glutamate ionotropic receptor delta type subunit 2 Names glutamate receptor ionotropic, delta-2 gluR delta-2 subunit glutamate receptor delta-2 subunit
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 4 Map Location: 4q31
Summary	interacts with the PDZ-domain protein S-SCAM/MAGI-2 in cerebellar Purkiinje cells [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_024379.1	NP_077355.1	glutamate receptor ionotropic, delta-2 precursor
XM_017592903.1	XP_017448392.1	glutamate receptor ionotropic, delta-2 isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	GRID2	XP_002688191.3
Danio rerio (zebrafish)	grid2	NP_001004123.1
Pan troglodytes (chimpanzee)	GRID2	NP_001158175.1
Rattus norvegicus (Norway rat)	Grid2	NP_077355.1
Gallus gallus (chicken)	GRID2	XP_420483.3
Macaca mulatta (Rhesus monkey)	GRID2	XP_001102156.1
Canis lupus familiaris (dog)	GRID2	XP_544985.3
Xenopus tropicalis (tropical clawed frog)	grid2	XP_002937281.2
Homo sapiens (human)	GRID2	NP_001501.2
Mus musculus (house mouse)	Grid2	NP_032193.1

Gene Ontology
Bibliography

Related articles in PubMed

The low binding affinity of D-serine at the ionotropic glutamate receptor GluD2 can be attributed to the hinge region.
Tapken D, Steffensen TB, Leth R, Kristensen LB, Gerbola A, Gajhede M, Jørgensen FS, Olsen L, Kastrup JS
Scientific reports746145(2017 04)

Conformational Plasticity in the Transsynaptic Neurexin-Cerebellin-Glutamate Receptor Adhesion Complex.
Cheng S, Seven AB, Wang J, Skiniotis G, Özkan E
Structure (London, England : 1993)24(12)2163-2173(2016 12)

Developmental Hypothyroxinemia and Hypothyroidism Reduce Parallel Fiber-Purkinje Cell Synapses in Rat Offspring by Downregulation of Neurexin1/Cbln1/GluD2 Tripartite Complex.
Wang Y, Dong J, Wang Y, Wei W, Song B, Shan Z, Teng W, Chen J
Biological trace element research173(2)465-74(2016 Oct)

Structural basis for integration of GluD receptors within synaptic organizer complexes.
Elegheert J, Kakegawa W, Clay JE, Shanks NF, Behiels E, Matsuda K, Kohda K, Miura E, Rossmann M, Mitakidis N, Motohashi J, Chang VT, Siebold C, Greger IH, Nakagawa T, Yuzaki M, Aricescu AR
Science (New York, N.Y.)353(6296)295-9(2016 Jul)

Cbln family proteins promote synapse formation by regulating distinct neurexin signaling pathways in various brain regions.
Matsuda K, Yuzaki M
The European journal of neuroscience33(8)1447-61(2011 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Grid2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Grid2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa61014	XM_017592903.1 Latest version!	Rattus norvegicus glutamate ionotropic receptor delta type subunit 2 (Grid2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ORa06989	NM_024379.1 Latest version!	Rattus norvegicus glutamate ionotropic receptor delta type subunit 2 (Grid2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Grid2 ( XM_017592903.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017448392.1 Rattus norvegicus glutamate ionotropic receptor delta type subunit 2 (Grid2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa61014
Clone ID Related Accession (Same CDS sequence)	XM_017592903.1
Accession Version	XM_017592903.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2760bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	glutamate receptor ionotropic, delta-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGTTT TCCCCTTGCT CTTCTTTCTA TCCTTCTGGT GGTCTCGAAC CTGGGACCTG 
GCGACCTCCG ATTCCATCAT CCACATCGGA GCAATTTTTG ATGAATCTGC TAAAAAAGAT 
GATGAAGTAT TCCGCACAGC AGTTGGTGAC CTCAACCAGA ATGAGGAAAT CTTACAGACT 
GAGAAAATCA CGTTTTCAGT GACATTTGTG GATGGCAACA ACCCTTTTCA AGCTGTTCAA 
GAAGCATGTG AACTTATGAA CCAGGGCATC TTGGCCTTGG TCAGCTCCAT TGGTTGCACA 
TCTGCTGGGT CCCTCCAGTC TTTGGCAGAC GCCATGCATA TCCCTCACCT CTTCATTCAG 
CGTTCAACAG CTGGGACCCC AAGAAGTGGC TGCGGCCTCA CCAGGAGCAA CAGAAACGAT 
GACTATACCC TTTCAGTTCG TCCACCTGTC TACTTGAATG AAGTCATCCT AAGAGTAGTC 
ACAGAGTATG CATGGCAGAA ATTTATTATC TTCTATGATA GTGAATATGA TATCCGTGGC 
ATACAGGAAT TTTTGGACAA AGTTTCCCAG CAGGGAATGG ATGTTGCCCT TCAAAAGGTG 
GAAAACAACA TCAATAAAAT GATCACCACG CTCTTTGACA CCATGAGGAT AGAGGAGTTG 
AATCGCTATC GAGACACTCT CAGAAGAGCC ATCCTTGTTA TGAACCCCGC CACAGCCAAA 
TCCTTCATAA GTGAGGTGGT GGAGACTAAT CTGGTTGCTT TTGACTGTCA CTGGATCATC 
ATCAATGAGG AAATAAATGA TGTGGATGTT CAGGAACTTG TCAGAAGGTC CATTGGAAGG 
TTAACAATTA TTCGGCAGAC ATTTCCAGTC CCCCAGAATA TAAGTCAGCG CTGTTTCCGT 
GGCAACCATC GAATTTCTTC AACACTGTGT GATCCCAAGG ATCCCTTCGC ACAGAATATG 
GAGATTTCTA ACCTTTACAT CTATGACACG GTGCTTCTGC TTGCAAACGC CTTCCATAAG 
AAGCTGGAGG ACCGGAAGTG GCACAGCATG GCGAGCTTGT CCTGTATCAG GAAAAACTCC 
AAGCCCTGGC AGGGAGGGCG GTCCATGCTG GAGACCATCA AGAAGGGTGG AGTTAATGGA 
TTGACTGGAG ATCTAGAATT TGGAGAAAAT GGAGGTAACC CCAATGTCCA CTTCGAAATC 
CTTGGAACCA ACTATGGAGA AGAACTTGGC AGGGGTGTCC GTAAACTTGG GTGCTGGAAT 
CCTGTCACAG GTCTGAATGG ATCACTGACA GACAAGAAAC TGGAGAATAA CATGCGAGGT 
GTGGTTCTAC GCGTGGTGAC TGTATTGGAG GAGCCTTTTG TAATGGTCTC TGAAAATGTC 
TTGGGAAAAC CGAAGAAATA CCAGGGCTTC TCCATTGATG TTTTAGATGC CCTATCTAAC 
TATCTGGGTT TTAACTATGA AATTTATGTT GCACCGGACC ACAAATATGG AAGCCCACAA 
GAAGATGGGA CATGGAATGG ACTAGTAGGA GAACTTGTTT TTAAGAGAGC TGACATCGGG 
ATTTCAGCTT TAACCATCAC TCCAGACCGG GAGAATGTTG TGGATTTTAC AACACGCTAC 
ATGGACTACT CAGTAGGGGT CCTACTGCGA AGGGCAGAAA AAACAGTGGA TATGTTTGCC 
TGCCTTGCAC CATTTGATCT ATCTCTGTGG GCCTGCATTG CCGGCACAGT CCTTCTTGTG 
GGACTCCTGG TCTACCTCTT GAACTGGCTT AATCCCCCAC GGTTGCAAAT GGGATCAATG 
ACATCTACTA CACTCTATAA CTCCATGTGG TTTGTGTACG GGTCCTTTGT ACAGCAAGGT 
GGGGAAGTAC CATACACAAC TTTGGCAACC CGAATGATGA TGGGGGCTTG GTGGTTATTT 
GCTCTGATTG TCATCTCATC TTATACAGCA AATCTTGCCG CTTTCCTCAC TATCACCCGC 
ATTGAGAGCT CCATCCAGTC TCTTCAGGAT CTGTCCAAGC AAACGGATAT CCCTTATGGC 
ACAGTCTTGG ATTCCGCAGT ATATCAGCAT GTTCGCATGA AGGGGCTGAA TCCTTTTGAG 
AGGGACAGCA TGTATTCCCA GATGTGGAGG ATGATCAACC GAAGCAATGG CTCAGAAAAC 
AATGTTCTGG AGTCCCAAGC AGGCATTCAA AAGGTAAAAT ATGGAAACTA TGCTTTTGTA 
TGGGATGCAG CTGTGTTGGA ATATGTGGCC ATCAATGACC CCGACTGTTC CTTCTACACT 
GTTGGAAATA CTGTTGCTGA CCGGGGGTAT GGCATCGCAC TGCAGCATGG CAGTCCCTAC 
CGTGATGTCT TTTCACAAAG GATCCTGGAG CTGCAGCAGA GTGGCGACAT GGACATCCTC 
AAGCACAAAT GGTGGCCTAA GAATGGCCAG TGTGACCTGT ACTCCTCAGT AGATGCAAAG 
CAGAAGGGAG GAGCCCTGGA CATCAAGAGT CTGGCAGGCG TGTTCTGCAT CCTGGCCGCG 
GGGATTGTAC TCTCCTGCCT CATAGCCGTC CTCGAGACAT GGTGGAGTCG GCGGAAGGGC 
TCCCGAGTCC CATCGAAAGA GGCTCCAGCC CTGCCAAATG AAGCTGCAGT GTCTGAAAGA 
ACCAATGCTT GGCCGCTAAG GCCGTGTCTG AAGCTTGGCG GTAATGACTG GAAAAACCAG 
GCATCCAGCC ATGGCCTACA CCACTCTACA CAGCTAGGAA GGCCTCTTGA TGACCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017448392.1
CDS	942..3701
Translation	MEVFPLLFFLSFWWSRTWDLATSDSIIHIGAIFDESAKKDDEVFRTAVGDLNQNEEILQTEKITFSVTFVDGNNPFQAVQEACELMNQGILALVSSIGCTSAGSLQSLADAMHIPHLFIQRSTAGTPRSGCGLTRSNRNDDYTLSVRPPVYLNEVILRVVTEYAWQKFIIFYDSEYDIRGIQEFLDKVSQQGMDVALQKVENNINKMITTLFDTMRIEELNRYRDTLRRAILVMNPATAKSFISEVVETNLVAFDCHWIIINEEINDVDVQELVRRSIGRLTIIRQTFPVPQNISQRCFRGNHRISSTLCDPKDPFAQNMEISNLYIYDTVLLLANAFHKKLEDRKWHSMASLSCIRKNSKPWQGGRSMLETIKKGGVNGLTGDLEFGENGGNPNVHFEILGTNYGEELGRGVRKLGCWNPVTGLNGSLTDKKLENNMRGVVLRVVTVLEEPFVMVSENVLGKPKKYQGFSIDVLDALSNYLGFNYEIYVAPDHKYGSPQEDGTWNGLVGELVFKRADIGISALTITPDRENVVDFTTRYMDYSVGVLLRRAEKTVDMFACLAPFDLSLWACIAGTVLLVGLLVYLLNWLNPPRLQMGSMTSTTLYNSMWFVYGSFVQQGGEVPYTTLATRMMMGAWWLFALIVISSYTANLAAFLTITRIESSIQSLQDLSKQTDIPYGTVLDSAVYQHVRMKGLNPFERDSMYSQMWRMINRSNGSENNVLESQAGIQKVKYGNYAFVWDAAVLEYVAINDPDCSFYTVGNTVADRGYGIALQHGSPYRDVFSQRILELQQSGDMDILKHKWWPKNGQCDLYSSVDAKQKGGALDIKSLAGVFCILAAGIVLSCLIAVLETWWSRRKGSRVPSKEAPALPNEAAVSERTNAWPLRPCLKLGGNDWKNQASSHGLHHSTQLGRPLDDP

Target ORF information:

RefSeq Version	XM_017592903.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glutamate ionotropic receptor delta type subunit 2 (Grid2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017592903.1

ORF Insert Sequence:

ATGGAAGTTT TCCCCTTGCT CTTCTTTCTA TCCTTCTGGT GGTCTCGAAC CTGGGACCTG 
GCGACCTCCG ATTCCATCAT CCACATCGGA GCAATTTTTG ATGAATCTGC TAAAAAAGAT 
GATGAAGTAT TCCGCACAGC AGTTGGTGAC CTCAACCAGA ATGAGGAAAT CTTACAGACT 
GAGAAAATCA CGTTTTCAGT GACATTTGTG GATGGCAACA ACCCTTTTCA AGCTGTTCAA 
GAAGCATGTG AACTTATGAA CCAGGGCATC TTGGCCTTGG TCAGCTCCAT TGGTTGCACA 
TCTGCTGGGT CCCTCCAGTC TTTGGCAGAC GCCATGCATA TCCCTCACCT CTTCATTCAG 
CGTTCAACAG CTGGGACCCC AAGAAGTGGC TGCGGCCTCA CCAGGAGCAA CAGAAACGAT 
GACTATACCC TTTCAGTTCG TCCACCTGTC TACTTGAATG AAGTCATCCT AAGAGTAGTC 
ACAGAGTATG CATGGCAGAA ATTTATTATC TTCTATGATA GTGAATATGA TATCCGTGGC 
ATACAGGAAT TTTTGGACAA AGTTTCCCAG CAGGGAATGG ATGTTGCCCT TCAAAAGGTG 
GAAAACAACA TCAATAAAAT GATCACCACG CTCTTTGACA CCATGAGGAT AGAGGAGTTG 
AATCGCTATC GAGACACTCT CAGAAGAGCC ATCCTTGTTA TGAACCCCGC CACAGCCAAA 
TCCTTCATAA GTGAGGTGGT GGAGACTAAT CTGGTTGCTT TTGACTGTCA CTGGATCATC 
ATCAATGAGG AAATAAATGA TGTGGATGTT CAGGAACTTG TCAGAAGGTC CATTGGAAGG 
TTAACAATTA TTCGGCAGAC ATTTCCAGTC CCCCAGAATA TAAGTCAGCG CTGTTTCCGT 
GGCAACCATC GAATTTCTTC AACACTGTGT GATCCCAAGG ATCCCTTCGC ACAGAATATG 
GAGATTTCTA ACCTTTACAT CTATGACACG GTGCTTCTGC TTGCAAACGC CTTCCATAAG 
AAGCTGGAGG ACCGGAAGTG GCACAGCATG GCGAGCTTGT CCTGTATCAG GAAAAACTCC 
AAGCCCTGGC AGGGAGGGCG GTCCATGCTG GAGACCATCA AGAAGGGTGG AGTTAATGGA 
TTGACTGGAG ATCTAGAATT TGGAGAAAAT GGAGGTAACC CCAATGTCCA CTTCGAAATC 
CTTGGAACCA ACTATGGAGA AGAACTTGGC AGGGGTGTCC GTAAACTTGG GTGCTGGAAT 
CCTGTCACAG GTCTGAATGG ATCACTGACA GACAAGAAAC TGGAGAATAA CATGCGAGGT 
GTGGTTCTAC GCGTGGTGAC TGTATTGGAG GAGCCTTTTG TAATGGTCTC TGAAAATGTC 
TTGGGAAAAC CGAAGAAATA CCAGGGCTTC TCCATTGATG TTTTAGATGC CCTATCTAAC 
TATCTGGGTT TTAACTATGA AATTTATGTT GCACCGGACC ACAAATATGG AAGCCCACAA 
GAAGATGGGA CATGGAATGG ACTAGTAGGA GAACTTGTTT TTAAGAGAGC TGACATCGGG 
ATTTCAGCTT TAACCATCAC TCCAGACCGG GAGAATGTTG TGGATTTTAC AACACGCTAC 
ATGGACTACT CAGTAGGGGT CCTACTGCGA AGGGCAGAAA AAACAGTGGA TATGTTTGCC 
TGCCTTGCAC CATTTGATCT ATCTCTGTGG GCCTGCATTG CCGGCACAGT CCTTCTTGTG 
GGACTCCTGG TCTACCTCTT GAACTGGCTT AATCCCCCAC GGTTGCAAAT GGGATCAATG 
ACATCTACTA CACTCTATAA CTCCATGTGG TTTGTGTACG GGTCCTTTGT ACAGCAAGGT 
GGGGAAGTAC CATACACAAC TTTGGCAACC CGAATGATGA TGGGGGCTTG GTGGTTATTT 
GCTCTGATTG TCATCTCATC TTATACAGCA AATCTTGCCG CTTTCCTCAC TATCACCCGC 
ATTGAGAGCT CCATCCAGTC TCTTCAGGAT CTGTCCAAGC AAACGGATAT CCCTTATGGC 
ACAGTCTTGG ATTCCGCAGT ATATCAGCAT GTTCGCATGA AGGGGCTGAA TCCTTTTGAG 
AGGGACAGCA TGTATTCCCA GATGTGGAGG ATGATCAACC GAAGCAATGG CTCAGAAAAC 
AATGTTCTGG AGTCCCAAGC AGGCATTCAA AAGGTAAAAT ATGGAAACTA TGCTTTTGTA 
TGGGATGCAG CTGTGTTGGA ATATGTGGCC ATCAATGACC CCGACTGTTC CTTCTACACT 
GTTGGAAATA CTGTTGCTGA CCGGGGGTAT GGCATCGCAC TGCAGCATGG CAGTCCCTAC 
CGTGATGTCT TTTCACAAAG GATCCTGGAG CTGCAGCAGA GTGGCGACAT GGACATCCTC 
AAGCACAAAT GGTGGCCTAA GAATGGCCAG TGTGACCTGT ACTCCTCAGT AGATGCAAAG 
CAGAAGGGAG GAGCCCTGGA CATCAAGAGT CTGGCAGGCG TGTTCTGCAT CCTGGCCGCG 
GGGATTGTAC TCTCCTGCCT CATAGCCGTC CTCGAGACAT GGTGGAGTCG GCGGAAGGGC 
TCCCGAGTCC CATCGAAAGA GGCTCCAGCC CTGCCAAATG AAGCTGCAGT GTCTGAAAGA 
ACCAATGCTT GGCCGCTAAG GCCGTGTCTG AAGCTTGGCG GTAATGACTG GAAAAACCAG 
GCATCCAGCC ATGGCCTACA CCACTCTACA CAGCTAGGAA GGCCTCTTGA TGACCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Grid2 ( NM_024379.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_077355.1 Rattus norvegicus glutamate ionotropic receptor delta type subunit 2 (Grid2), mRNA.

Price & Availability↑

CloneID	ORa06989
Clone ID Related Accession (Same CDS sequence)	NM_024379.1
Accession Version	NM_024379.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3024bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577203200000
Organism	Rattus norvegicus(Norway rat)
Product	glutamate receptor ionotropic, delta-2 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U08256.1. On Jan 12, 2005 this sequence version replaced XM_346701.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U08256.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGAAGTTT TCCCCTTGCT CTTCTTTCTA TCCTTCTGGT GGTCTCGAAC CTGGGACCTG 
GCGACCTCCG ATTCCATCAT CCACATCGGA GCAATTTTTG ATGAATCTGC TAAAAAAGAT 
GATGAAGTAT TCCGCACAGC AGTTGGTGAC CTCAACCAGA ATGAGGAAAT CTTACAGACT 
GAGAAAATCA CATTTTCAGT GACATTTGTG GATGGCAACA ACCCTTTTCA AGCTGTTCAA 
GAAGCATGTG AACTTATGAA CCAGGGCATC TTGGCCTTGG TCAGCTCCAT TGGTTGCACA 
TCTGCTGGGT CCCTCCAGTC TTTGGCAGAC GCCATGCATA TCCCTCACCT CTTCATTCAG 
CGTTCAACAG CTGGGACCCC AAGAAGTGGC TGCGGCCTCA CCAGGAGCAA CAGAAACGAT 
GACTATACCC TTTCAGTTCG TCCACCTGTC TACTTGAATG AAGTCATCCT AAGAGTAGTC 
ACAGAGTATG CATGGCAGAA ATTTATTATC TTCTATGATA GTGAATATGA TATCCGTGGC 
ATACAGGAAT TTTTGGACAA AGTTTCCCAG CAGGGAATGG ATGTTGCCCT TCAAAAGGTG 
GAAAACAACA TCAATAAAAT GATCACCACG CTCTTTGACA CCATGAGGAT AGAGGAGTTG 
AATCGCTATC GAGACACTCT CAGAAGAGCC ATCCTTGTTA TGAACCCCGC CACAGCCAAA 
TCCTTCATAA GTGAGGTGGT GGAGACTAAT CTGGTTGCTT TTGACTGTCA CTGGATCATC 
ATCAATGAGG AAATAAATGA TGTGGATGTT CAGGAACTTG TCAGAAGGTC CATTGGAAGG 
TTAACAATTA TTCGGCAGAC ATTTCCAGTC CCCCAGAATA TAAGTCAGCG CTGTTTCCGT 
GGCAACCATC GAATTTCTTC AACACTGTGT GATCCCAAGG ATCCCTTCGC ACAGAATATG 
GAGATTTCTA ACCTTTACAT CTATGACACG GTGCTTCTGC TTGCAAACGC CTTTCATAAG 
AAGCTGCAGG ACCGGAAGTG GCACAGCATG GCGAGCTTGT CCTGTATCAG GAAAAACTCC 
AAGCCCTGGC AGGGAGGGCG GTCCATGCTG GAGACCATCA AGAAGGGTGG AGTTAATGGA 
TTGACTGGAG ATCTAGAATT TGGAGAAAAT GGAGGTAACC CCAATGTCCA CTTCGAAATC 
CTTGGAACCA ACTATGGAGA AGAACTTGGC AGGGGTGTCC GTAAACTTGG GTGCTGGAAT 
CCTGTCACAG GTCTGAATGG ATCACTGACA GACAAGAAAC TGGAGAATAA CATGCGAGGT 
GTGGTTCTAC GCGTGGTGAC TGTATTGGAG GAGCCTTTTG TAATGGTCTC TGAAAATGTC 
TTGGGAAAAC CGAAGAAATA CCAGGGCTTC TCCATTGATG TTTTAGATGC CCTATCTAAC 
TATCTGGGTT TTAACTATGA AATTTATGTT GCACCGGACC ACAAATATGG AAGCCCACAA 
GAAGATGGGA CATGGAATGG ACTAGTAGGA GAACTTGTTT TTAAGAGAGC TGACATCGGG 
ATTTCAGCTT TAACCATCAC TCCAGACCGG GAGAATGTTG TGGATTTTAC AACACGCTAC 
ATGGACTACT CAGTAGGGGT CCTACTGCGA AGGGCAGAAA AAACAGTGGA TATGTTTGCC 
TGCCTTGCAC CATTTGATCT ATCTCTGTGG GCCTGCATTG CCGGCACAGT CCTTCTTGTG 
GGACTCCTGG TCTACCTCTT GAACTGGCTT AATCCCCCAC GGTTGCAAAT GGGATCAATG 
ACATCTACTA CACTCTATAA CTCCATGTGG TTTGTGTACG GGTCCTTTGT ACAGCAAGGT 
GGGGAAGTAC CATACACAAC TTTGGCAACC CGAATGATGA TGGGGGCTTG GTGGTTATTT 
GCTCTGATTG TCATCTCATC TTATACAGCA AATCTTGCCG CTTTCCTCAC TATCACCCGC 
ATTGAGAGCT CCATCCAGTC TCTTCAGGAT CTGTCCAAGC AAACGGATAT CCCTTATGGC 
ACAGTCTTGG ATTCCGCAGT ATATCAGCAT GTTCGCATGA AGGGGCTGAA TCCTTTTGAG 
AGGGACAGCA TGTATTCCCA GATGTGGAGG ATGATCAACC GAAGCAATGG CTCAGAGAAC 
AATGTTCTGG AGTCCCAAGC AGGCATTCAA AAGGTAAAAT ATGGAAACTA TGCTTTTGTA 
TGGGATGCAG CTGTGTTGGA ATATGTGGCC ATCAATGACC CCGACTGTTC CTTCTACACT 
GTTGGAAATA CTGTTGCTGA CCGGGGGTAT GGCATCGCAC TGCAGCATGG CAGTCCCTAC 
CGTGATGTCT TTTCACAAAG GATCCTGGAG CTGCAGCAGA GTGGCGACAT GGACATCCTC 
AAGCACAAAT GGTGGCCTAA GAATGGCCAG TGTGACCTGT ACTCCTCAGT AGATGCAAAG 
CAGAAGGGAG GAGCCCTGGA CATCAAGAGT CTGGCAGGCG TGTTCTGCAT CCTGGCCGCG 
GGGATTGTAC TCTCCTGCCT CATAGCCGTC CTCGAGACAT GGTGGAGTCG GCGGAAGGGC 
TCCCGAGTCC CATCGAAAGA GGATGACAAG GAAATTGACC TGGAGCACCT CCATAGACGT 
GTAAATAGCT TGTGCACAGA TGACGACAGC CCCCATAAAC AGTTTTCCAC CTCGTCAATT 
GACTTGACCC CTCTGGACAT TGACACTTTG CCAACACGAC AAGCCCTGGA GCAGATCAGT 
GATTTCAGAA ACACTCACAT CACCACCACC ACCTTCATCC CAGAGCAGAT CCAGACTCTT 
AGCCGCACAC TCTCAGCAAA AGCTGCCTTT GGCTTCACTT TCGGCAGTGT GCCCGAGCAC 
CGAACTGGCC CTTTTAGGCA CAGGGCCCCT AATGGGGGCT TTTTCAGGAG TCCTATCAAA 
ACAATGTCAT CTATTCCTTA TCAGCCTACT CCCACTCTGG GGCTCAATCT GGGCAATGAC 
CCCGACCGAG GCACGTCCAT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_077355.1
CDS	312..3335
Translation	MEVFPLLFFLSFWWSRTWDLATSDSIIHIGAIFDESAKKDDEVFRTAVGDLNQNEEILQTEKITFSVTFVDGNNPFQAVQEACELMNQGILALVSSIGCTSAGSLQSLADAMHIPHLFIQRSTAGTPRSGCGLTRSNRNDDYTLSVRPPVYLNEVILRVVTEYAWQKFIIFYDSEYDIRGIQEFLDKVSQQGMDVALQKVENNINKMITTLFDTMRIEELNRYRDTLRRAILVMNPATAKSFISEVVETNLVAFDCHWIIINEEINDVDVQELVRRSIGRLTIIRQTFPVPQNISQRCFRGNHRISSTLCDPKDPFAQNMEISNLYIYDTVLLLANAFHKKLQDRKWHSMASLSCIRKNSKPWQGGRSMLETIKKGGVNGLTGDLEFGENGGNPNVHFEILGTNYGEELGRGVRKLGCWNPVTGLNGSLTDKKLENNMRGVVLRVVTVLEEPFVMVSENVLGKPKKYQGFSIDVLDALSNYLGFNYEIYVAPDHKYGSPQEDGTWNGLVGELVFKRADIGISALTITPDRENVVDFTTRYMDYSVGVLLRRAEKTVDMFACLAPFDLSLWACIAGTVLLVGLLVYLLNWLNPPRLQMGSMTSTTLYNSMWFVYGSFVQQGGEVPYTTLATRMMMGAWWLFALIVISSYTANLAAFLTITRIESSIQSLQDLSKQTDIPYGTVLDSAVYQHVRMKGLNPFERDSMYSQMWRMINRSNGSENNVLESQAGIQKVKYGNYAFVWDAAVLEYVAINDPDCSFYTVGNTVADRGYGIALQHGSPYRDVFSQRILELQQSGDMDILKHKWWPKNGQCDLYSSVDAKQKGGALDIKSLAGVFCILAAGIVLSCLIAVLETWWSRRKGSRVPSKEDDKEIDLEHLHRRVNSLCTDDDSPHKQFSTSSIDLTPLDIDTLPTRQALEQISDFRNTHITTTTFIPEQIQTLSRTLSAKAAFGFTFGSVPEHRTGPFRHRAPNGGFFRSPIKTMSSIPYQPTPTLGLNLGNDPDRGTSI

Target ORF information:

RefSeq Version	NM_024379.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glutamate ionotropic receptor delta type subunit 2 (Grid2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024379.1

ORF Insert Sequence:

ATGGAAGTTT TCCCCTTGCT CTTCTTTCTA TCCTTCTGGT GGTCTCGAAC CTGGGACCTG 
GCGACCTCCG ATTCCATCAT CCACATCGGA GCAATTTTTG ATGAATCTGC TAAAAAAGAT 
GATGAAGTAT TCCGCACAGC AGTTGGTGAC CTCAACCAGA ATGAGGAAAT CTTACAGACT 
GAGAAAATCA CATTTTCAGT GACATTTGTG GATGGCAACA ACCCTTTTCA AGCTGTTCAA 
GAAGCATGTG AACTTATGAA CCAGGGCATC TTGGCCTTGG TCAGCTCCAT TGGTTGCACA 
TCTGCTGGGT CCCTCCAGTC TTTGGCAGAC GCCATGCATA TCCCTCACCT CTTCATTCAG 
CGTTCAACAG CTGGGACCCC AAGAAGTGGC TGCGGCCTCA CCAGGAGCAA CAGAAACGAT 
GACTATACCC TTTCAGTTCG TCCACCTGTC TACTTGAATG AAGTCATCCT AAGAGTAGTC 
ACAGAGTATG CATGGCAGAA ATTTATTATC TTCTATGATA GTGAATATGA TATCCGTGGC 
ATACAGGAAT TTTTGGACAA AGTTTCCCAG CAGGGAATGG ATGTTGCCCT TCAAAAGGTG 
GAAAACAACA TCAATAAAAT GATCACCACG CTCTTTGACA CCATGAGGAT AGAGGAGTTG 
AATCGCTATC GAGACACTCT CAGAAGAGCC ATCCTTGTTA TGAACCCCGC CACAGCCAAA 
TCCTTCATAA GTGAGGTGGT GGAGACTAAT CTGGTTGCTT TTGACTGTCA CTGGATCATC 
ATCAATGAGG AAATAAATGA TGTGGATGTT CAGGAACTTG TCAGAAGGTC CATTGGAAGG 
TTAACAATTA TTCGGCAGAC ATTTCCAGTC CCCCAGAATA TAAGTCAGCG CTGTTTCCGT 
GGCAACCATC GAATTTCTTC AACACTGTGT GATCCCAAGG ATCCCTTCGC ACAGAATATG 
GAGATTTCTA ACCTTTACAT CTATGACACG GTGCTTCTGC TTGCAAACGC CTTTCATAAG 
AAGCTGCAGG ACCGGAAGTG GCACAGCATG GCGAGCTTGT CCTGTATCAG GAAAAACTCC 
AAGCCCTGGC AGGGAGGGCG GTCCATGCTG GAGACCATCA AGAAGGGTGG AGTTAATGGA 
TTGACTGGAG ATCTAGAATT TGGAGAAAAT GGAGGTAACC CCAATGTCCA CTTCGAAATC 
CTTGGAACCA ACTATGGAGA AGAACTTGGC AGGGGTGTCC GTAAACTTGG GTGCTGGAAT 
CCTGTCACAG GTCTGAATGG ATCACTGACA GACAAGAAAC TGGAGAATAA CATGCGAGGT 
GTGGTTCTAC GCGTGGTGAC TGTATTGGAG GAGCCTTTTG TAATGGTCTC TGAAAATGTC 
TTGGGAAAAC CGAAGAAATA CCAGGGCTTC TCCATTGATG TTTTAGATGC CCTATCTAAC 
TATCTGGGTT TTAACTATGA AATTTATGTT GCACCGGACC ACAAATATGG AAGCCCACAA 
GAAGATGGGA CATGGAATGG ACTAGTAGGA GAACTTGTTT TTAAGAGAGC TGACATCGGG 
ATTTCAGCTT TAACCATCAC TCCAGACCGG GAGAATGTTG TGGATTTTAC AACACGCTAC 
ATGGACTACT CAGTAGGGGT CCTACTGCGA AGGGCAGAAA AAACAGTGGA TATGTTTGCC 
TGCCTTGCAC CATTTGATCT ATCTCTGTGG GCCTGCATTG CCGGCACAGT CCTTCTTGTG 
GGACTCCTGG TCTACCTCTT GAACTGGCTT AATCCCCCAC GGTTGCAAAT GGGATCAATG 
ACATCTACTA CACTCTATAA CTCCATGTGG TTTGTGTACG GGTCCTTTGT ACAGCAAGGT 
GGGGAAGTAC CATACACAAC TTTGGCAACC CGAATGATGA TGGGGGCTTG GTGGTTATTT 
GCTCTGATTG TCATCTCATC TTATACAGCA AATCTTGCCG CTTTCCTCAC TATCACCCGC 
ATTGAGAGCT CCATCCAGTC TCTTCAGGAT CTGTCCAAGC AAACGGATAT CCCTTATGGC 
ACAGTCTTGG ATTCCGCAGT ATATCAGCAT GTTCGCATGA AGGGGCTGAA TCCTTTTGAG 
AGGGACAGCA TGTATTCCCA GATGTGGAGG ATGATCAACC GAAGCAATGG CTCAGAGAAC 
AATGTTCTGG AGTCCCAAGC AGGCATTCAA AAGGTAAAAT ATGGAAACTA TGCTTTTGTA 
TGGGATGCAG CTGTGTTGGA ATATGTGGCC ATCAATGACC CCGACTGTTC CTTCTACACT 
GTTGGAAATA CTGTTGCTGA CCGGGGGTAT GGCATCGCAC TGCAGCATGG CAGTCCCTAC 
CGTGATGTCT TTTCACAAAG GATCCTGGAG CTGCAGCAGA GTGGCGACAT GGACATCCTC 
AAGCACAAAT GGTGGCCTAA GAATGGCCAG TGTGACCTGT ACTCCTCAGT AGATGCAAAG 
CAGAAGGGAG GAGCCCTGGA CATCAAGAGT CTGGCAGGCG TGTTCTGCAT CCTGGCCGCG 
GGGATTGTAC TCTCCTGCCT CATAGCCGTC CTCGAGACAT GGTGGAGTCG GCGGAAGGGC 
TCCCGAGTCC CATCGAAAGA GGATGACAAG GAAATTGACC TGGAGCACCT CCATAGACGT 
GTAAATAGCT TGTGCACAGA TGACGACAGC CCCCATAAAC AGTTTTCCAC CTCGTCAATT 
GACTTGACCC CTCTGGACAT TGACACTTTG CCAACACGAC AAGCCCTGGA GCAGATCAGT 
GATTTCAGAA ACACTCACAT CACCACCACC ACCTTCATCC CAGAGCAGAT CCAGACTCTT 
AGCCGCACAC TCTCAGCAAA AGCTGCCTTT GGCTTCACTT TCGGCAGTGT GCCCGAGCAC 
CGAACTGGCC CTTTTAGGCA CAGGGCCCCT AATGGGGGCT TTTTCAGGAG TCCTATCAAA 
ACAATGTCAT CTATTCCTTA TCAGCCTACT CCCACTCTGG GGCTCAATCT GGGCAATGAC 
CCCGACCGAG GCACGTCCAT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The low binding affinity of D-serine at the ionotropic glutamate receptor GluD2 can be attributed to the hinge region.
Scientific reports746145(2017 04)
Tapken D,Steffensen TB,Leth R,Kristensen LB,Gerbola A,Gajhede M,Jørgensen FS,Olsen L,Kastrup JS

Conformational Plasticity in the Transsynaptic Neurexin-Cerebellin-Glutamate Receptor Adhesion Complex.
Structure (London, England : 1993)24(12)2163-2173(2016 12)
Cheng S,Seven AB,Wang J,Skiniotis G,Özkan E

Developmental Hypothyroxinemia and Hypothyroidism Reduce Parallel Fiber-Purkinje Cell Synapses in Rat Offspring by Downregulation of Neurexin1/Cbln1/GluD2 Tripartite Complex.
Biological trace element research173(2)465-74(2016 Oct)
Wang Y,Dong J,Wang Y,Wei W,Song B,Shan Z,Teng W,Chen J

Structural basis for integration of GluD receptors within synaptic organizer complexes.
Science (New York, N.Y.)353(6296)295-9(2016 Jul)
Elegheert J,Kakegawa W,Clay JE,Shanks NF,Behiels E,Matsuda K,Kohda K,Miura E,Rossmann M,Mitakidis N,Motohashi J,Chang VT,Siebold C,Greger IH,Nakagawa T,Yuzaki M,Aricescu AR

Cbln family proteins promote synapse formation by regulating distinct neurexin signaling pathways in various brain regions.
The European journal of neuroscience33(8)1447-61(2011 Apr)
Matsuda K,Yuzaki M

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Grid2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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