Asic1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Asic1
Entrez Gene ID	79123
Full Name	acid sensing ion channel subunit 1
Synonyms	Accn2
General protein information	Preferred Names acid sensing ion channel subunit 1 Names acid-sensing ion channel 1 BNaC2 acid-sensing (proton-gated) ion channel 1 amiloride-sensitive cation channel 2, neuronal brain sodium channel 2 proton gated cation channel ASIC1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q36
Summary	forms a proton-gated ion channel that may play a role in sensory neuron function and pain perception [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006257378.3	XP_006257440.1	acid-sensing ion channel 1 isoform X2
XM_017595126.1	XP_017450615.1	acid-sensing ion channel 1 isoform X3
NM_024154.2	NP_077068.1	acid-sensing ion channel 1
XM_017595124.1	XP_017450613.1	acid-sensing ion channel 1 isoform X1
XM_017595125.1	XP_017450614.1	acid-sensing ion channel 1 isoform X3
XM_017595127.1	XP_017450616.1	acid-sensing ion channel 1 isoform X3
XM_017595129.1	XP_017450618.1	acid-sensing ion channel 1 isoform X5
XM_017595128.1	XP_017450617.1	acid-sensing ion channel 1 isoform X4

Pathways from BioSystems

Homologies

Homo sapiens (human)	ASIC1	NP_064423.2
Canis lupus familiaris (dog)	ASIC1	XP_005636919.1
Bos taurus (cattle)	ACCN2	NP_001179862.1
Rattus norvegicus (Norway rat)	Asic1	NP_077068.1
Macaca mulatta (Rhesus monkey)	ACCN2	XP_002798614.1
Gallus gallus (chicken)	ACCN2	NP_001035557.1
Danio rerio (zebrafish)	asic1a	NP_999955.1
Caenorhabditis elegans (roundworm)	del-10	NP_495302.3
Pan troglodytes (chimpanzee)	ACCN2	XP_509053.2
Mus musculus (house mouse)	Asic1	NP_033727.1
Xenopus tropicalis (tropical clawed frog)	asic1	XP_002935779.1

Gene Ontology
Bibliography

Related articles in PubMed

Inhibition of acid‑sensing ion channel 1a attenuates acid‑induced activation of autophagy via a calcium signaling pathway in articular chondrocytes.
Gao WF, Xu YY, Ge JF, Chen FH
International journal of molecular medicine43(4)1778-1788(2019 Apr)

The role of Ca
Wu X, Ren G, Zhou R, Ge J, Chen FH
Laboratory investigation; a journal of technical methods and pathology99(4)499-513(2019 04)

Effects of autophagy on acid-sensing ion channel 1a-mediated apoptosis in rat articular chondrocytes.
Xie YY, Li Y, Zhou RP, Dai BB, Qian YJ, Wu XS, Ge JF, Hu W, Chen FH
Molecular and cellular biochemistry443(1-2)181-191(2018 Jun)

ASIC1a contributes to the symptom of pain in a rat model of chronic prostatitis.
Fan S, Hao ZY, Zhang L, Zhou J, Zhang YF, Tai S, Zhang XS, Liang CZ
Asian journal of andrology20(3)300-305(2018 May-Jun)

Dual actions of Psalmotoxin at ASIC1a and ASIC2a heteromeric channels (ASIC1a/2a).
Liu Y, Hagan R, Schoellerman J
Scientific reports8(1)7179(2018 05)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Asic1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Asic1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa62165	XM_017595128.1 Latest version!	Rattus norvegicus acid sensing ion channel subunit 1 (Asic1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ORa62166	XM_017595129.1 Latest version!	Rattus norvegicus acid sensing ion channel subunit 1 (Asic1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ORa13137	NM_024154.2 Latest version!	Rattus norvegicus acid sensing ion channel subunit 1 (Asic1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ORa40149	XM_006257378.3 Latest version!	Rattus norvegicus acid sensing ion channel subunit 1 (Asic1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ORa62163	XM_017595124.1 Latest version!	Rattus norvegicus acid sensing ion channel subunit 1 (Asic1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ORa62164	XM_017595125.1 Latest version!	Rattus norvegicus acid sensing ion channel subunit 1 (Asic1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ORa62164	XM_017595127.1 Latest version!	Rattus norvegicus acid sensing ion channel subunit 1 (Asic1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ORa62164	XM_017595126.1 Latest version!	Rattus norvegicus acid sensing ion channel subunit 1 (Asic1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Asic1 ( XM_017595128.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017450617.1 Rattus norvegicus acid sensing ion channel subunit 1 (Asic1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ORa62165
Clone ID Related Accession (Same CDS sequence)	XM_017595128.1
Accession Version	XM_017595128.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1341bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	acid-sensing ion channel 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCATCC AGATCTTTTG TTCTGTGTCA TTCTCCTCTG GAGAGGAGGC CCCGGGATCC 
ATGGCAGATA TCTGGGGTCC CCACCACCAC CGGCAGCAGC AGGACAGCTC AGAATCGGAA 
GAAGAGGAAG AGAAGGAAAT GGAGGCAGGG TCGGAGTTGG ATGAGGGTGA TGACTCACCT 
AGGGACTTGG TGGCCTTCGC CAACAGCTGT ACCCTCCATG GTGCCAGCCA TGTGTTTGTG 
GAAGGGGGCC CAGGGCCAAG GCAGGCCTTA TGGGCAGTGG CCTTTGTCAT AGCACTGGGT 
GCCTTCCTGT GCCAGGTCTT CACTCGGTAT GGGAAGTGTT ACACATTCAA CTCGGGCCAA 
GATGGGCGGC CACGGCTGAA GACCATGAAA GGTGGGACTG GCAATGGCCT GGAGATCATG 
CTGGACATTC AGCAAGATGA ATATTTGCCT GTGTGGGGAG AGACCGACGA GACATCCTTC 
GAAGCAGGCA TCAAAGTGCA GATCCACAGT CAGGATGAAC CCCCTTTCAT CGACCAGCTG 
GGCTTTGGTG TGGCTCCAGG TTTCCAGACG TTTGTGTCTT GCCAGGAGCA GAGGCAGCTC 
ATCTACCTGC CCTCACCCTG GGGCACCTGC AATGCTGTTA CCATGGACTC GGATTTCTTC 
GACTCCTACA GCATCACTGC CTGCCGGATT GATTGCGAGA CGCGTTACCT GGTGGAGAAC 
TGCAACTGCC GTATGGTGCA CATGCCAGGG GACGCCCCAT ACTGCACTCC AGAGCAGTAC 
AAGGAGTGTG CAGATCCTGC CCTGGACTTC CTAGTGGAGA AAGACCAGGA ATACTGCGTG 
TGTGAGATGC CTTGCAACCT GACCCGCTAC GGCAAGGAGC TGTCCATGGT CAAGATCCCA 
AGCAAAGCCT CCGCCAAGTA CCTGGCCAAG AAGTTCAACA AATCGGAGCA GTACATAGGG 
GAGAACATTC TGGTGCTGGA CATTTTCTTT GAAGTCCTCA ACTATGAGAC CATCGAGCAG 
AAAAAGGCCT ATGAGATCGC AGGGCTGTTG GGTGACATCG GGGGCCAGAT GGGGTTGTTC 
ATCGGTGCCA GCATCCTCAC CGTGCTGGAA CTCTTTGACT ATGCCTACGA GGTCATTAAG 
CACAGGCTGT GCAGACGTGG AAAGTGCCAG AAGGAGGCTA AGAGGAGCAG CGCAGACAAG 
GGCGTGGCGC TCAGCCTGGA TGACGTCAAA AGACACAATC CCTGCGAGAG CCTCCGAGGA 
CATCCTGCCG GGATGACGTA CGCTGCCAAC ATCCTACCTC ACCATCCCGC TCGAGGCACG 
TTTGAGGACT TTACCTGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017450617.1
CDS	235..1575
Translation	MPIQIFCSVSFSSGEEAPGSMADIWGPHHHRQQQDSSESEEEEEKEMEAGSELDEGDDSPRDLVAFANSCTLHGASHVFVEGGPGPRQALWAVAFVIALGAFLCQVFTRYGKCYTFNSGQDGRPRLKTMKGGTGNGLEIMLDIQQDEYLPVWGETDETSFEAGIKVQIHSQDEPPFIDQLGFGVAPGFQTFVSCQEQRQLIYLPSPWGTCNAVTMDSDFFDSYSITACRIDCETRYLVENCNCRMVHMPGDAPYCTPEQYKECADPALDFLVEKDQEYCVCEMPCNLTRYGKELSMVKIPSKASAKYLAKKFNKSEQYIGENILVLDIFFEVLNYETIEQKKAYEIAGLLGDIGGQMGLFIGASILTVLELFDYAYEVIKHRLCRRGKCQKEAKRSSADKGVALSLDDVKRHNPCESLRGHPAGMTYAANILPHHPARGTFEDFTC

Target ORF information:

RefSeq Version	XM_017595128.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus acid sensing ion channel subunit 1 (Asic1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017595128.1

ORF Insert Sequence:

ATGCCCATCC AGATCTTTTG TTCTGTGTCA TTCTCCTCTG GAGAGGAGGC CCCGGGATCC 
ATGGCAGATA TCTGGGGTCC CCACCACCAC CGGCAGCAGC AGGACAGCTC AGAATCGGAA 
GAAGAGGAAG AGAAGGAAAT GGAGGCAGGG TCGGAGTTGG ATGAGGGTGA TGACTCACCT 
AGGGACTTGG TGGCCTTCGC CAACAGCTGT ACCCTCCATG GTGCCAGCCA TGTGTTTGTG 
GAAGGGGGCC CAGGGCCAAG GCAGGCCTTA TGGGCAGTGG CCTTTGTCAT AGCACTGGGT 
GCCTTCCTGT GCCAGGTCTT CACTCGGTAT GGGAAGTGTT ACACATTCAA CTCGGGCCAA 
GATGGGCGGC CACGGCTGAA GACCATGAAA GGTGGGACTG GCAATGGCCT GGAGATCATG 
CTGGACATTC AGCAAGATGA ATATTTGCCT GTGTGGGGAG AGACCGACGA GACATCCTTC 
GAAGCAGGCA TCAAAGTGCA GATCCACAGT CAGGATGAAC CCCCTTTCAT CGACCAGCTG 
GGCTTTGGTG TGGCTCCAGG TTTCCAGACG TTTGTGTCTT GCCAGGAGCA GAGGCAGCTC 
ATCTACCTGC CCTCACCCTG GGGCACCTGC AATGCTGTTA CCATGGACTC GGATTTCTTC 
GACTCCTACA GCATCACTGC CTGCCGGATT GATTGCGAGA CGCGTTACCT GGTGGAGAAC 
TGCAACTGCC GTATGGTGCA CATGCCAGGG GACGCCCCAT ACTGCACTCC AGAGCAGTAC 
AAGGAGTGTG CAGATCCTGC CCTGGACTTC CTAGTGGAGA AAGACCAGGA ATACTGCGTG 
TGTGAGATGC CTTGCAACCT GACCCGCTAC GGCAAGGAGC TGTCCATGGT CAAGATCCCA 
AGCAAAGCCT CCGCCAAGTA CCTGGCCAAG AAGTTCAACA AATCGGAGCA GTACATAGGG 
GAGAACATTC TGGTGCTGGA CATTTTCTTT GAAGTCCTCA ACTATGAGAC CATCGAGCAG 
AAAAAGGCCT ATGAGATCGC AGGGCTGTTG GGTGACATCG GGGGCCAGAT GGGGTTGTTC 
ATCGGTGCCA GCATCCTCAC CGTGCTGGAA CTCTTTGACT ATGCCTACGA GGTCATTAAG 
CACAGGCTGT GCAGACGTGG AAAGTGCCAG AAGGAGGCTA AGAGGAGCAG CGCAGACAAG 
GGCGTGGCGC TCAGCCTGGA TGACGTCAAA AGACACAATC CCTGCGAGAG CCTCCGAGGA 
CATCCTGCCG GGATGACGTA CGCTGCCAAC ATCCTACCTC ACCATCCCGC TCGAGGCACG 
TTTGAGGACT TTACCTGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Asic1 ( XM_017595129.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017450618.1 Rattus norvegicus acid sensing ion channel subunit 1 (Asic1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	ORa62166
Clone ID Related Accession (Same CDS sequence)	XM_017595129.1
Accession Version	XM_017595129.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1338bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	acid-sensing ion channel 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCATCC AGATCTTTTG TTCTGTGTCA TTCTCCTCTG GAGAGGAGGC CCCGGGATCC 
ATGGCAGATA TCTGGGGTCC CCACCACCAC CGGCAGCAGC AGGACAGCTC AGAATCGGAA 
GAAGAGGAAG AGAAGGAAAT GGAGGCAGGG TCGGAGTTGG ATGAGGGTGA TGACTCACCT 
AGGGACTTGG TGGCCTTCGC CAACAGCTGT ACCCTCCATG GTGCCAGCCA TGTGTTTGTG 
GAAGGGGGCC CAGGGCCAAG GCAGGCCTTA TGGGCAGTGG CCTTTGTCAT AGCACTGGGT 
GCCTTCCTGT GCCAGGTCTT CACTCGGTAT GGGAAGTGTT ACACATTCAA CTCGGGCCAA 
GATGGGCGGC CACGGCTGAA GACCATGAAA GGTGGGACTG GCAATGGCCT GGAGATCATG 
CTGGACATTC AGCAAGATGA ATATTTGCCT GTGTGGGGAG AGACCGACGA GACATCCTTC 
GAAGCAGGCA TCAAAGTGCA GATCCACAGT CAGGATGAAC CCCCTTTCAT CGACCAGCTG 
GGCTTTGGTG TGGCTCCAGG TTTCCAGACG TTTGTGTCTT GCCAGGAGCA GAGGCTCATC 
TACCTGCCCT CACCCTGGGG CACCTGCAAT GCTGTTACCA TGGACTCGGA TTTCTTCGAC 
TCCTACAGCA TCACTGCCTG CCGGATTGAT TGCGAGACGC GTTACCTGGT GGAGAACTGC 
AACTGCCGTA TGGTGCACAT GCCAGGGGAC GCCCCATACT GCACTCCAGA GCAGTACAAG 
GAGTGTGCAG ATCCTGCCCT GGACTTCCTA GTGGAGAAAG ACCAGGAATA CTGCGTGTGT 
GAGATGCCTT GCAACCTGAC CCGCTACGGC AAGGAGCTGT CCATGGTCAA GATCCCAAGC 
AAAGCCTCCG CCAAGTACCT GGCCAAGAAG TTCAACAAAT CGGAGCAGTA CATAGGGGAG 
AACATTCTGG TGCTGGACAT TTTCTTTGAA GTCCTCAACT ATGAGACCAT CGAGCAGAAA 
AAGGCCTATG AGATCGCAGG GCTGTTGGGT GACATCGGGG GCCAGATGGG GTTGTTCATC 
GGTGCCAGCA TCCTCACCGT GCTGGAACTC TTTGACTATG CCTACGAGGT CATTAAGCAC 
AGGCTGTGCA GACGTGGAAA GTGCCAGAAG GAGGCTAAGA GGAGCAGCGC AGACAAGGGC 
GTGGCGCTCA GCCTGGATGA CGTCAAAAGA CACAATCCCT GCGAGAGCCT CCGAGGACAT 
CCTGCCGGGA TGACGTACGC TGCCAACATC CTACCTCACC ATCCCGCTCG AGGCACGTTT 
GAGGACTTTA CCTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017450618.1
CDS	212..1549
Translation	MPIQIFCSVSFSSGEEAPGSMADIWGPHHHRQQQDSSESEEEEEKEMEAGSELDEGDDSPRDLVAFANSCTLHGASHVFVEGGPGPRQALWAVAFVIALGAFLCQVFTRYGKCYTFNSGQDGRPRLKTMKGGTGNGLEIMLDIQQDEYLPVWGETDETSFEAGIKVQIHSQDEPPFIDQLGFGVAPGFQTFVSCQEQRLIYLPSPWGTCNAVTMDSDFFDSYSITACRIDCETRYLVENCNCRMVHMPGDAPYCTPEQYKECADPALDFLVEKDQEYCVCEMPCNLTRYGKELSMVKIPSKASAKYLAKKFNKSEQYIGENILVLDIFFEVLNYETIEQKKAYEIAGLLGDIGGQMGLFIGASILTVLELFDYAYEVIKHRLCRRGKCQKEAKRSSADKGVALSLDDVKRHNPCESLRGHPAGMTYAANILPHHPARGTFEDFTC

Target ORF information:

RefSeq Version	XM_017595129.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus acid sensing ion channel subunit 1 (Asic1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017595129.1

ORF Insert Sequence:

ATGCCCATCC AGATCTTTTG TTCTGTGTCA TTCTCCTCTG GAGAGGAGGC CCCGGGATCC 
ATGGCAGATA TCTGGGGTCC CCACCACCAC CGGCAGCAGC AGGACAGCTC AGAATCGGAA 
GAAGAGGAAG AGAAGGAAAT GGAGGCAGGG TCGGAGTTGG ATGAGGGTGA TGACTCACCT 
AGGGACTTGG TGGCCTTCGC CAACAGCTGT ACCCTCCATG GTGCCAGCCA TGTGTTTGTG 
GAAGGGGGCC CAGGGCCAAG GCAGGCCTTA TGGGCAGTGG CCTTTGTCAT AGCACTGGGT 
GCCTTCCTGT GCCAGGTCTT CACTCGGTAT GGGAAGTGTT ACACATTCAA CTCGGGCCAA 
GATGGGCGGC CACGGCTGAA GACCATGAAA GGTGGGACTG GCAATGGCCT GGAGATCATG 
CTGGACATTC AGCAAGATGA ATATTTGCCT GTGTGGGGAG AGACCGACGA GACATCCTTC 
GAAGCAGGCA TCAAAGTGCA GATCCACAGT CAGGATGAAC CCCCTTTCAT CGACCAGCTG 
GGCTTTGGTG TGGCTCCAGG TTTCCAGACG TTTGTGTCTT GCCAGGAGCA GAGGCTCATC 
TACCTGCCCT CACCCTGGGG CACCTGCAAT GCTGTTACCA TGGACTCGGA TTTCTTCGAC 
TCCTACAGCA TCACTGCCTG CCGGATTGAT TGCGAGACGC GTTACCTGGT GGAGAACTGC 
AACTGCCGTA TGGTGCACAT GCCAGGGGAC GCCCCATACT GCACTCCAGA GCAGTACAAG 
GAGTGTGCAG ATCCTGCCCT GGACTTCCTA GTGGAGAAAG ACCAGGAATA CTGCGTGTGT 
GAGATGCCTT GCAACCTGAC CCGCTACGGC AAGGAGCTGT CCATGGTCAA GATCCCAAGC 
AAAGCCTCCG CCAAGTACCT GGCCAAGAAG TTCAACAAAT CGGAGCAGTA CATAGGGGAG 
AACATTCTGG TGCTGGACAT TTTCTTTGAA GTCCTCAACT ATGAGACCAT CGAGCAGAAA 
AAGGCCTATG AGATCGCAGG GCTGTTGGGT GACATCGGGG GCCAGATGGG GTTGTTCATC 
GGTGCCAGCA TCCTCACCGT GCTGGAACTC TTTGACTATG CCTACGAGGT CATTAAGCAC 
AGGCTGTGCA GACGTGGAAA GTGCCAGAAG GAGGCTAAGA GGAGCAGCGC AGACAAGGGC 
GTGGCGCTCA GCCTGGATGA CGTCAAAAGA CACAATCCCT GCGAGAGCCT CCGAGGACAT 
CCTGCCGGGA TGACGTACGC TGCCAACATC CTACCTCACC ATCCCGCTCG AGGCACGTTT 
GAGGACTTTA CCTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Asic1 ( NM_024154.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_077068.1 Rattus norvegicus acid sensing ion channel subunit 1 (Asic1), mRNA.

Price & Availability↑

CloneID	ORa13137
Clone ID Related Accession (Same CDS sequence)	NM_024154.2
Accession Version	NM_024154.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1581bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1572105600000
Organism	Rattus norvegicus(Norway rat)
Product	acid-sensing ion channel 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from U94403.1, CO398759.1, AJ309926.1 and DV721841.1. On Apr 5, 2007 this sequence version replaced NM_024154.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGAATTGA AGACCGAGGA GGAGGAGGTG GGTGGTGTCC AGCCGGTGAG CATCCAGGCT 
TTCGCCAGCA GCTCCACGCT GCATGGTCTT GCCCACATCT TCTCCTATGA GCGGCTGTCT 
CTGAAGCGGG CACTGTGGGC CCTGTGCTTC CTGGGTTCGC TGGCCGTCCT GCTGTGTGTG 
TGCACTGAGC GTGTGCAGTA CTACTTCTGC TATCACCACG TCACCAAGCT TGACGAAGTG 
GCTGCCTCCC AGCTCACCTT CCCTGCTGTC ACACTGTGCA ATCTCAATGA GTTCCGCTTT 
AGCCAAGTCT CCAAGAATGA CCTGTACCAT GCTGGGGAGC TGCTGGCCCT GCTCAACAAC 
AGGTATGAGA TCCCGGACAC ACAGATGGCT GATGAAAAGC AGCTAGAGAT ATTGCAGGAC 
AAGGCCAACT TCCGGAGCTT CAAGCCCAAG CCCTTCAACA TGCGTGAATT CTACGACAGA 
GCGGGGCACG ATATTCGAGA CATGCTGCTC TCGTGCCACT TCCGTGGGGA GGCCTGCAGC 
GCTGAAGATT TCAAAGTGGT CTTCACTCGG TATGGGAAGT GTTACACATT CAACTCGGGC 
CAAGATGGGC GGCCACGGCT GAAGACCATG AAAGGTGGGA CTGGCAATGG CCTGGAGATC 
ATGCTGGACA TTCAGCAAGA TGAATATTTG CCTGTGTGGG GAGAGACCGA CGAGACATCC 
TTCGAAGCAG GCATCAAAGT GCAGATCCAC AGTCAGGATG AACCCCCTTT CATCGACCAG 
CTGGGCTTTG GTGTGGCTCC AGGTTTCCAG ACGTTTGTGT CTTGCCAGGA GCAGAGGCTC 
ATCTACCTGC CCTCACCCTG GGGCACCTGC AATGCTGTTA CCATGGACTC GGATTTCTTC 
GACTCCTACA GCATCACTGC CTGCCGGATT GATTGCGAGA CGCGTTACCT GGTGGAGAAC 
TGCAACTGCC GTATGGTGCA CATGCCAGGG GACGCCCCAT ACTGCACTCC AGAGCAGTAC 
AAGGAGTGTG CAGATCCTGC CCTGGACTTC CTAGTGGAGA AAGACCAGGA ATACTGCGTG 
TGTGAGATGC CTTGCAACCT GACCCGCTAC GGCAAGGAGC TGTCCATGGT CAAGATCCCA 
AGCAAAGCCT CCGCCAAGTA CCTGGCCAAG AAGTTCAACA AATCGGAGCA GTACATAGGG 
GAGAACATTC TGGTGCTGGA CATTTTCTTT GAAGTCCTCA ACTATGAGAC CATCGAGCAG 
AAAAAGGCCT ATGAGATCGC AGGGCTGTTG GGTGACATCG GGGGCCAGAT GGGGTTGTTC 
ATCGGTGCCA GCATCCTCAC CGTGCTGGAA CTCTTTGACT ATGCCTACGA GGTCATTAAG 
CACAGGCTGT GCAGACGTGG AAAGTGCCAG AAGGAGGCTA AGAGGAGCAG CGCAGACAAG 
GGCGTGGCGC TCAGCCTGGA TGACGTCAAA AGACACAATC CCTGCGAGAG CCTCCGAGGA 
CATCCTGCCG GGATGACGTA CGCTGCCAAC ATCCTACCTC ACCATCCCGC TCGAGGCACG 
TTTGAGGACT TTACCTGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_077068.1
CDS	123..1703
Translation	MELKTEEEEVGGVQPVSIQAFASSSTLHGLAHIFSYERLSLKRALWALCFLGSLAVLLCVCTERVQYYFCYHHVTKLDEVAASQLTFPAVTLCNLNEFRFSQVSKNDLYHAGELLALLNNRYEIPDTQMADEKQLEILQDKANFRSFKPKPFNMREFYDRAGHDIRDMLLSCHFRGEACSAEDFKVVFTRYGKCYTFNSGQDGRPRLKTMKGGTGNGLEIMLDIQQDEYLPVWGETDETSFEAGIKVQIHSQDEPPFIDQLGFGVAPGFQTFVSCQEQRLIYLPSPWGTCNAVTMDSDFFDSYSITACRIDCETRYLVENCNCRMVHMPGDAPYCTPEQYKECADPALDFLVEKDQEYCVCEMPCNLTRYGKELSMVKIPSKASAKYLAKKFNKSEQYIGENILVLDIFFEVLNYETIEQKKAYEIAGLLGDIGGQMGLFIGASILTVLELFDYAYEVIKHRLCRRGKCQKEAKRSSADKGVALSLDDVKRHNPCESLRGHPAGMTYAANILPHHPARGTFEDFTC

Target ORF information:

RefSeq Version	NM_024154.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus acid sensing ion channel subunit 1 (Asic1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024154.2

ORF Insert Sequence:

ATGGAATTGA AGACCGAGGA GGAGGAGGTG GGTGGTGTCC AGCCGGTGAG CATCCAGGCT 
TTCGCCAGCA GCTCCACGCT GCATGGTCTT GCCCACATCT TCTCCTATGA GCGGCTGTCT 
CTGAAGCGGG CACTGTGGGC CCTGTGCTTC CTGGGTTCGC TGGCCGTCCT GCTGTGTGTG 
TGCACTGAGC GTGTGCAGTA CTACTTCTGC TATCACCACG TCACCAAGCT TGACGAAGTG 
GCTGCCTCCC AGCTCACCTT CCCTGCTGTC ACACTGTGCA ATCTCAATGA GTTCCGCTTT 
AGCCAAGTCT CCAAGAATGA CCTGTACCAT GCTGGGGAGC TGCTGGCCCT GCTCAACAAC 
AGGTATGAGA TCCCGGACAC ACAGATGGCT GATGAAAAGC AGCTAGAGAT ATTGCAGGAC 
AAGGCCAACT TCCGGAGCTT CAAGCCCAAG CCCTTCAACA TGCGTGAATT CTACGACAGA 
GCGGGGCACG ATATTCGAGA CATGCTGCTC TCGTGCCACT TCCGTGGGGA GGCCTGCAGC 
GCTGAAGATT TCAAAGTGGT CTTCACTCGG TATGGGAAGT GTTACACATT CAACTCGGGC 
CAAGATGGGC GGCCACGGCT GAAGACCATG AAAGGTGGGA CTGGCAATGG CCTGGAGATC 
ATGCTGGACA TTCAGCAAGA TGAATATTTG CCTGTGTGGG GAGAGACCGA CGAGACATCC 
TTCGAAGCAG GCATCAAAGT GCAGATCCAC AGTCAGGATG AACCCCCTTT CATCGACCAG 
CTGGGCTTTG GTGTGGCTCC AGGTTTCCAG ACGTTTGTGT CTTGCCAGGA GCAGAGGCTC 
ATCTACCTGC CCTCACCCTG GGGCACCTGC AATGCTGTTA CCATGGACTC GGATTTCTTC 
GACTCCTACA GCATCACTGC CTGCCGGATT GATTGCGAGA CGCGTTACCT GGTGGAGAAC 
TGCAACTGCC GTATGGTGCA CATGCCAGGG GACGCCCCAT ACTGCACTCC AGAGCAGTAC 
AAGGAGTGTG CAGATCCTGC CCTGGACTTC CTAGTGGAGA AAGACCAGGA ATACTGCGTG 
TGTGAGATGC CTTGCAACCT GACCCGCTAC GGCAAGGAGC TGTCCATGGT CAAGATCCCA 
AGCAAAGCCT CCGCCAAGTA CCTGGCCAAG AAGTTCAACA AATCGGAGCA GTACATAGGG 
GAGAACATTC TGGTGCTGGA CATTTTCTTT GAAGTCCTCA ACTATGAGAC CATCGAGCAG 
AAAAAGGCCT ATGAGATCGC AGGGCTGTTG GGTGACATCG GGGGCCAGAT GGGGTTGTTC 
ATCGGTGCCA GCATCCTCAC CGTGCTGGAA CTCTTTGACT ATGCCTACGA GGTCATTAAG 
CACAGGCTGT GCAGACGTGG AAAGTGCCAG AAGGAGGCTA AGAGGAGCAG CGCAGACAAG 
GGCGTGGCGC TCAGCCTGGA TGACGTCAAA AGACACAATC CCTGCGAGAG CCTCCGAGGA 
CATCCTGCCG GGATGACGTA CGCTGCCAAC ATCCTACCTC ACCATCCCGC TCGAGGCACG 
TTTGAGGACT TTACCTGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Asic1 ( XM_006257378.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006257440.1 Rattus norvegicus acid sensing ion channel subunit 1 (Asic1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa40149
Clone ID Related Accession (Same CDS sequence)	XM_006257378.3
Accession Version	XM_006257378.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1680bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	acid-sensing ion channel 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006257378.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCATCC AGATCTTTTG TTCTGTGTCA TTCTCCTCTG GAGAGGAGGC CCCGGGATCC 
ATGGCAGATA TCTGGGGTCC CCACCACCAC CGGCAGCAGC AGGACAGCTC AGAATCGGAA 
GAAGAGGAAG AGAAGGAAAT GGAGGCAGGG TCGGAGTTGG ATGAGGGTGA TGACTCACCT 
AGGGACTTGG TGGCCTTCGC CAACAGCTGT ACCCTCCATG GTGCCAGCCA TGTGTTTGTG 
GAAGGGGGCC CAGGGCCAAG GCAGGCCTTA TGGGCAGTGG CCTTTGTCAT AGCACTGGGT 
GCCTTCCTGT GCCAGGTAGG GGACCGCGTT GCTTATTACC TCAGCTACCC ACACGTGACT 
TTGCTAGACG AAGTGGCCAC CACGGAGCTG GTCTTCCCAG CTGTCACCTT CTGCAACACC 
AATGCCGTGC GGTTGTCCCA GCTCAGCTAC CCTGACTTGC TCTACCTGGC CCCCATGCTA 
GGACTGGATG AGAGTGATGA CCCCGGGGTG CCCCTTGCTC CTCCTGGCCC AGAGGCTTTC 
TCCGGGGAGC CTTTTAACCT CCATCGTTTC TATAATCGCT CTTGCCACCG GCTGGAGGAC 
ATGCTGCTCT ATTGTTCCTA CTGTGGGGGC CCCTGTGGTC CCCACAACTT CTCAGTGGTC 
TTCACTCGGT ATGGGAAGTG TTACACATTC AACTCGGGCC AAGATGGGCG GCCACGGCTG 
AAGACCATGA AAGGTGGGAC TGGCAATGGC CTGGAGATCA TGCTGGACAT TCAGCAAGAT 
GAATATTTGC CTGTGTGGGG AGAGACCGAC GAGACATCCT TCGAAGCAGG CATCAAAGTG 
CAGATCCACA GTCAGGATGA ACCCCCTTTC ATCGACCAGC TGGGCTTTGG TGTGGCTCCA 
GGTTTCCAGA CGTTTGTGTC TTGCCAGGAG CAGAGGCTCA TCTACCTGCC CTCACCCTGG 
GGCACCTGCA ATGCTGTTAC CATGGACTCG GATTTCTTCG ACTCCTACAG CATCACTGCC 
TGCCGGATTG ATTGCGAGAC GCGTTACCTG GTGGAGAACT GCAACTGCCG TATGGTGCAC 
ATGCCAGGGG ACGCCCCATA CTGCACTCCA GAGCAGTACA AGGAGTGTGC AGATCCTGCC 
CTGGACTTCC TAGTGGAGAA AGACCAGGAA TACTGCGTGT GTGAGATGCC TTGCAACCTG 
ACCCGCTACG GCAAGGAGCT GTCCATGGTC AAGATCCCAA GCAAAGCCTC CGCCAAGTAC 
CTGGCCAAGA AGTTCAACAA ATCGGAGCAG TACATAGGGG AGAACATTCT GGTGCTGGAC 
ATTTTCTTTG AAGTCCTCAA CTATGAGACC ATCGAGCAGA AAAAGGCCTA TGAGATCGCA 
GGGCTGTTGG GTGACATCGG GGGCCAGATG GGGTTGTTCA TCGGTGCCAG CATCCTCACC 
GTGCTGGAAC TCTTTGACTA TGCCTACGAG GTCATTAAGC ACAGGCTGTG CAGACGTGGA 
AAGTGCCAGA AGGAGGCTAA GAGGAGCAGC GCAGACAAGG GCGTGGCGCT CAGCCTGGAT 
GACGTCAAAA GACACAATCC CTGCGAGAGC CTCCGAGGAC ATCCTGCCGG GATGACGTAC 
GCTGCCAACA TCCTACCTCA CCATCCCGCT CGAGGCACGT TTGAGGACTT TACCTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006257440.1
CDS	266..1945
Translation	MPIQIFCSVSFSSGEEAPGSMADIWGPHHHRQQQDSSESEEEEEKEMEAGSELDEGDDSPRDLVAFANSCTLHGASHVFVEGGPGPRQALWAVAFVIALGAFLCQVGDRVAYYLSYPHVTLLDEVATTELVFPAVTFCNTNAVRLSQLSYPDLLYLAPMLGLDESDDPGVPLAPPGPEAFSGEPFNLHRFYNRSCHRLEDMLLYCSYCGGPCGPHNFSVVFTRYGKCYTFNSGQDGRPRLKTMKGGTGNGLEIMLDIQQDEYLPVWGETDETSFEAGIKVQIHSQDEPPFIDQLGFGVAPGFQTFVSCQEQRLIYLPSPWGTCNAVTMDSDFFDSYSITACRIDCETRYLVENCNCRMVHMPGDAPYCTPEQYKECADPALDFLVEKDQEYCVCEMPCNLTRYGKELSMVKIPSKASAKYLAKKFNKSEQYIGENILVLDIFFEVLNYETIEQKKAYEIAGLLGDIGGQMGLFIGASILTVLELFDYAYEVIKHRLCRRGKCQKEAKRSSADKGVALSLDDVKRHNPCESLRGHPAGMTYAANILPHHPARGTFEDFTC

Target ORF information:

RefSeq Version	XM_006257378.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus acid sensing ion channel subunit 1 (Asic1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006257378.3

ORF Insert Sequence:

ATGCCCATCC AGATCTTTTG TTCTGTGTCA TTCTCCTCTG GAGAGGAGGC CCCGGGATCC 
ATGGCAGATA TCTGGGGTCC CCACCACCAC CGGCAGCAGC AGGACAGCTC AGAATCGGAA 
GAAGAGGAAG AGAAGGAAAT GGAGGCAGGG TCGGAGTTGG ATGAGGGTGA TGACTCACCT 
AGGGACTTGG TGGCCTTCGC CAACAGCTGT ACCCTCCATG GTGCCAGCCA TGTGTTTGTG 
GAAGGGGGCC CAGGGCCAAG GCAGGCCTTA TGGGCAGTGG CCTTTGTCAT AGCACTGGGT 
GCCTTCCTGT GCCAGGTAGG GGACCGCGTT GCTTATTACC TCAGCTACCC ACACGTGACT 
TTGCTAGACG AAGTGGCCAC CACGGAGCTG GTCTTCCCAG CTGTCACCTT CTGCAACACC 
AATGCCGTGC GGTTGTCCCA GCTCAGCTAC CCTGACTTGC TCTACCTGGC CCCCATGCTA 
GGACTGGATG AGAGTGATGA CCCCGGGGTG CCCCTTGCTC CTCCTGGCCC AGAGGCTTTC 
TCCGGGGAGC CTTTTAACCT CCATCGTTTC TATAATCGCT CTTGCCACCG GCTGGAGGAC 
ATGCTGCTCT ATTGTTCCTA CTGTGGGGGC CCCTGTGGTC CCCACAACTT CTCAGTGGTC 
TTCACTCGGT ATGGGAAGTG TTACACATTC AACTCGGGCC AAGATGGGCG GCCACGGCTG 
AAGACCATGA AAGGTGGGAC TGGCAATGGC CTGGAGATCA TGCTGGACAT TCAGCAAGAT 
GAATATTTGC CTGTGTGGGG AGAGACCGAC GAGACATCCT TCGAAGCAGG CATCAAAGTG 
CAGATCCACA GTCAGGATGA ACCCCCTTTC ATCGACCAGC TGGGCTTTGG TGTGGCTCCA 
GGTTTCCAGA CGTTTGTGTC TTGCCAGGAG CAGAGGCTCA TCTACCTGCC CTCACCCTGG 
GGCACCTGCA ATGCTGTTAC CATGGACTCG GATTTCTTCG ACTCCTACAG CATCACTGCC 
TGCCGGATTG ATTGCGAGAC GCGTTACCTG GTGGAGAACT GCAACTGCCG TATGGTGCAC 
ATGCCAGGGG ACGCCCCATA CTGCACTCCA GAGCAGTACA AGGAGTGTGC AGATCCTGCC 
CTGGACTTCC TAGTGGAGAA AGACCAGGAA TACTGCGTGT GTGAGATGCC TTGCAACCTG 
ACCCGCTACG GCAAGGAGCT GTCCATGGTC AAGATCCCAA GCAAAGCCTC CGCCAAGTAC 
CTGGCCAAGA AGTTCAACAA ATCGGAGCAG TACATAGGGG AGAACATTCT GGTGCTGGAC 
ATTTTCTTTG AAGTCCTCAA CTATGAGACC ATCGAGCAGA AAAAGGCCTA TGAGATCGCA 
GGGCTGTTGG GTGACATCGG GGGCCAGATG GGGTTGTTCA TCGGTGCCAG CATCCTCACC 
GTGCTGGAAC TCTTTGACTA TGCCTACGAG GTCATTAAGC ACAGGCTGTG CAGACGTGGA 
AAGTGCCAGA AGGAGGCTAA GAGGAGCAGC GCAGACAAGG GCGTGGCGCT CAGCCTGGAT 
GACGTCAAAA GACACAATCC CTGCGAGAGC CTCCGAGGAC ATCCTGCCGG GATGACGTAC 
GCTGCCAACA TCCTACCTCA CCATCCCGCT CGAGGCACGT TTGAGGACTT TACCTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Asic1 ( XM_017595124.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017450613.1 Rattus norvegicus acid sensing ion channel subunit 1 (Asic1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa62163
Clone ID Related Accession (Same CDS sequence)	XM_017595124.1
Accession Version	XM_017595124.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1683bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	acid-sensing ion channel 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCATCC AGATCTTTTG TTCTGTGTCA TTCTCCTCTG GAGAGGAGGC CCCGGGATCC 
ATGGCAGATA TCTGGGGTCC CCACCACCAC CGGCAGCAGC AGGACAGCTC AGAATCGGAA 
GAAGAGGAAG AGAAGGAAAT GGAGGCAGGG TCGGAGTTGG ATGAGGGTGA TGACTCACCT 
AGGGACTTGG TGGCCTTCGC CAACAGCTGT ACCCTCCATG GTGCCAGCCA TGTGTTTGTG 
GAAGGGGGCC CAGGGCCAAG GCAGGCCTTA TGGGCAGTGG CCTTTGTCAT AGCACTGGGT 
GCCTTCCTGT GCCAGGTAGG GGACCGCGTT GCTTATTACC TCAGCTACCC ACACGTGACT 
TTGCTAGACG AAGTGGCCAC CACGGAGCTG GTCTTCCCAG CTGTCACCTT CTGCAACACC 
AATGCCGTGC GGTTGTCCCA GCTCAGCTAC CCTGACTTGC TCTACCTGGC CCCCATGCTA 
GGACTGGATG AGAGTGATGA CCCCGGGGTG CCCCTTGCTC CTCCTGGCCC AGAGGCTTTC 
TCCGGGGAGC CTTTTAACCT CCATCGTTTC TATAATCGCT CTTGCCACCG GCTGGAGGAC 
ATGCTGCTCT ATTGTTCCTA CTGTGGGGGC CCCTGTGGTC CCCACAACTT CTCAGTGGTC 
TTCACTCGGT ATGGGAAGTG TTACACATTC AACTCGGGCC AAGATGGGCG GCCACGGCTG 
AAGACCATGA AAGGTGGGAC TGGCAATGGC CTGGAGATCA TGCTGGACAT TCAGCAAGAT 
GAATATTTGC CTGTGTGGGG AGAGACCGAC GAGACATCCT TCGAAGCAGG CATCAAAGTG 
CAGATCCACA GTCAGGATGA ACCCCCTTTC ATCGACCAGC TGGGCTTTGG TGTGGCTCCA 
GGTTTCCAGA CGTTTGTGTC TTGCCAGGAG CAGAGGCAGC TCATCTACCT GCCCTCACCC 
TGGGGCACCT GCAATGCTGT TACCATGGAC TCGGATTTCT TCGACTCCTA CAGCATCACT 
GCCTGCCGGA TTGATTGCGA GACGCGTTAC CTGGTGGAGA ACTGCAACTG CCGTATGGTG 
CACATGCCAG GGGACGCCCC ATACTGCACT CCAGAGCAGT ACAAGGAGTG TGCAGATCCT 
GCCCTGGACT TCCTAGTGGA GAAAGACCAG GAATACTGCG TGTGTGAGAT GCCTTGCAAC 
CTGACCCGCT ACGGCAAGGA GCTGTCCATG GTCAAGATCC CAAGCAAAGC CTCCGCCAAG 
TACCTGGCCA AGAAGTTCAA CAAATCGGAG CAGTACATAG GGGAGAACAT TCTGGTGCTG 
GACATTTTCT TTGAAGTCCT CAACTATGAG ACCATCGAGC AGAAAAAGGC CTATGAGATC 
GCAGGGCTGT TGGGTGACAT CGGGGGCCAG ATGGGGTTGT TCATCGGTGC CAGCATCCTC 
ACCGTGCTGG AACTCTTTGA CTATGCCTAC GAGGTCATTA AGCACAGGCT GTGCAGACGT 
GGAAAGTGCC AGAAGGAGGC TAAGAGGAGC AGCGCAGACA AGGGCGTGGC GCTCAGCCTG 
GATGACGTCA AAAGACACAA TCCCTGCGAG AGCCTCCGAG GACATCCTGC CGGGATGACG 
TACGCTGCCA ACATCCTACC TCACCATCCC GCTCGAGGCA CGTTTGAGGA CTTTACCTGC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017450613.1
CDS	283..1965
Translation	MPIQIFCSVSFSSGEEAPGSMADIWGPHHHRQQQDSSESEEEEEKEMEAGSELDEGDDSPRDLVAFANSCTLHGASHVFVEGGPGPRQALWAVAFVIALGAFLCQVGDRVAYYLSYPHVTLLDEVATTELVFPAVTFCNTNAVRLSQLSYPDLLYLAPMLGLDESDDPGVPLAPPGPEAFSGEPFNLHRFYNRSCHRLEDMLLYCSYCGGPCGPHNFSVVFTRYGKCYTFNSGQDGRPRLKTMKGGTGNGLEIMLDIQQDEYLPVWGETDETSFEAGIKVQIHSQDEPPFIDQLGFGVAPGFQTFVSCQEQRQLIYLPSPWGTCNAVTMDSDFFDSYSITACRIDCETRYLVENCNCRMVHMPGDAPYCTPEQYKECADPALDFLVEKDQEYCVCEMPCNLTRYGKELSMVKIPSKASAKYLAKKFNKSEQYIGENILVLDIFFEVLNYETIEQKKAYEIAGLLGDIGGQMGLFIGASILTVLELFDYAYEVIKHRLCRRGKCQKEAKRSSADKGVALSLDDVKRHNPCESLRGHPAGMTYAANILPHHPARGTFEDFTC

Target ORF information:

RefSeq Version	XM_017595124.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus acid sensing ion channel subunit 1 (Asic1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017595124.1

ORF Insert Sequence:

ATGCCCATCC AGATCTTTTG TTCTGTGTCA TTCTCCTCTG GAGAGGAGGC CCCGGGATCC 
ATGGCAGATA TCTGGGGTCC CCACCACCAC CGGCAGCAGC AGGACAGCTC AGAATCGGAA 
GAAGAGGAAG AGAAGGAAAT GGAGGCAGGG TCGGAGTTGG ATGAGGGTGA TGACTCACCT 
AGGGACTTGG TGGCCTTCGC CAACAGCTGT ACCCTCCATG GTGCCAGCCA TGTGTTTGTG 
GAAGGGGGCC CAGGGCCAAG GCAGGCCTTA TGGGCAGTGG CCTTTGTCAT AGCACTGGGT 
GCCTTCCTGT GCCAGGTAGG GGACCGCGTT GCTTATTACC TCAGCTACCC ACACGTGACT 
TTGCTAGACG AAGTGGCCAC CACGGAGCTG GTCTTCCCAG CTGTCACCTT CTGCAACACC 
AATGCCGTGC GGTTGTCCCA GCTCAGCTAC CCTGACTTGC TCTACCTGGC CCCCATGCTA 
GGACTGGATG AGAGTGATGA CCCCGGGGTG CCCCTTGCTC CTCCTGGCCC AGAGGCTTTC 
TCCGGGGAGC CTTTTAACCT CCATCGTTTC TATAATCGCT CTTGCCACCG GCTGGAGGAC 
ATGCTGCTCT ATTGTTCCTA CTGTGGGGGC CCCTGTGGTC CCCACAACTT CTCAGTGGTC 
TTCACTCGGT ATGGGAAGTG TTACACATTC AACTCGGGCC AAGATGGGCG GCCACGGCTG 
AAGACCATGA AAGGTGGGAC TGGCAATGGC CTGGAGATCA TGCTGGACAT TCAGCAAGAT 
GAATATTTGC CTGTGTGGGG AGAGACCGAC GAGACATCCT TCGAAGCAGG CATCAAAGTG 
CAGATCCACA GTCAGGATGA ACCCCCTTTC ATCGACCAGC TGGGCTTTGG TGTGGCTCCA 
GGTTTCCAGA CGTTTGTGTC TTGCCAGGAG CAGAGGCAGC TCATCTACCT GCCCTCACCC 
TGGGGCACCT GCAATGCTGT TACCATGGAC TCGGATTTCT TCGACTCCTA CAGCATCACT 
GCCTGCCGGA TTGATTGCGA GACGCGTTAC CTGGTGGAGA ACTGCAACTG CCGTATGGTG 
CACATGCCAG GGGACGCCCC ATACTGCACT CCAGAGCAGT ACAAGGAGTG TGCAGATCCT 
GCCCTGGACT TCCTAGTGGA GAAAGACCAG GAATACTGCG TGTGTGAGAT GCCTTGCAAC 
CTGACCCGCT ACGGCAAGGA GCTGTCCATG GTCAAGATCC CAAGCAAAGC CTCCGCCAAG 
TACCTGGCCA AGAAGTTCAA CAAATCGGAG CAGTACATAG GGGAGAACAT TCTGGTGCTG 
GACATTTTCT TTGAAGTCCT CAACTATGAG ACCATCGAGC AGAAAAAGGC CTATGAGATC 
GCAGGGCTGT TGGGTGACAT CGGGGGCCAG ATGGGGTTGT TCATCGGTGC CAGCATCCTC 
ACCGTGCTGG AACTCTTTGA CTATGCCTAC GAGGTCATTA AGCACAGGCT GTGCAGACGT 
GGAAAGTGCC AGAAGGAGGC TAAGAGGAGC AGCGCAGACA AGGGCGTGGC GCTCAGCCTG 
GATGACGTCA AAAGACACAA TCCCTGCGAG AGCCTCCGAG GACATCCTGC CGGGATGACG 
TACGCTGCCA ACATCCTACC TCACCATCCC GCTCGAGGCA CGTTTGAGGA CTTTACCTGC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Asic1 ( XM_017595126.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017450615.1 Rattus norvegicus acid sensing ion channel subunit 1 (Asic1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa62164
Clone ID Related Accession (Same CDS sequence)	XM_017595126.1 , XM_017595125.1 , XM_017595127.1
Accession Version	XM_017595126.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1584bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	acid-sensing ion channel 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATTGA AGACCGAGGA GGAGGAGGTG GGTGGTGTCC AGCCGGTGAG CATCCAGGCT 
TTCGCCAGCA GCTCCACGCT GCATGGTCTT GCCCACATCT TCTCCTATGA GCGGCTGTCT 
CTGAAGCGGG CACTGTGGGC CCTGTGCTTC CTGGGTTCGC TGGCCGTCCT GCTGTGTGTG 
TGCACTGAGC GTGTGCAGTA CTACTTCTGC TATCACCACG TCACCAAGCT TGACGAAGTG 
GCTGCCTCCC AGCTCACCTT CCCTGCTGTC ACACTGTGCA ATCTCAATGA GTTCCGCTTT 
AGCCAAGTCT CCAAGAATGA CCTGTACCAT GCTGGGGAGC TGCTGGCCCT GCTCAACAAC 
AGGTATGAGA TCCCGGACAC ACAGATGGCT GATGAAAAGC AGCTAGAGAT ATTGCAGGAC 
AAGGCCAACT TCCGGAGCTT CAAGCCCAAG CCCTTCAACA TGCGTGAATT CTACGACAGA 
GCGGGGCACG ATATTCGAGA CATGCTGCTC TCGTGCCACT TCCGTGGGGA GGCCTGCAGC 
GCTGAAGATT TCAAAGTGGT CTTCACTCGG TATGGGAAGT GTTACACATT CAACTCGGGC 
CAAGATGGGC GGCCACGGCT GAAGACCATG AAAGGTGGGA CTGGCAATGG CCTGGAGATC 
ATGCTGGACA TTCAGCAAGA TGAATATTTG CCTGTGTGGG GAGAGACCGA CGAGACATCC 
TTCGAAGCAG GCATCAAAGT GCAGATCCAC AGTCAGGATG AACCCCCTTT CATCGACCAG 
CTGGGCTTTG GTGTGGCTCC AGGTTTCCAG ACGTTTGTGT CTTGCCAGGA GCAGAGGCAG 
CTCATCTACC TGCCCTCACC CTGGGGCACC TGCAATGCTG TTACCATGGA CTCGGATTTC 
TTCGACTCCT ACAGCATCAC TGCCTGCCGG ATTGATTGCG AGACGCGTTA CCTGGTGGAG 
AACTGCAACT GCCGTATGGT GCACATGCCA GGGGACGCCC CATACTGCAC TCCAGAGCAG 
TACAAGGAGT GTGCAGATCC TGCCCTGGAC TTCCTAGTGG AGAAAGACCA GGAATACTGC 
GTGTGTGAGA TGCCTTGCAA CCTGACCCGC TACGGCAAGG AGCTGTCCAT GGTCAAGATC 
CCAAGCAAAG CCTCCGCCAA GTACCTGGCC AAGAAGTTCA ACAAATCGGA GCAGTACATA 
GGGGAGAACA TTCTGGTGCT GGACATTTTC TTTGAAGTCC TCAACTATGA GACCATCGAG 
CAGAAAAAGG CCTATGAGAT CGCAGGGCTG TTGGGTGACA TCGGGGGCCA GATGGGGTTG 
TTCATCGGTG CCAGCATCCT CACCGTGCTG GAACTCTTTG ACTATGCCTA CGAGGTCATT 
AAGCACAGGC TGTGCAGACG TGGAAAGTGC CAGAAGGAGG CTAAGAGGAG CAGCGCAGAC 
AAGGGCGTGG CGCTCAGCCT GGATGACGTC AAAAGACACA ATCCCTGCGA GAGCCTCCGA 
GGACATCCTG CCGGGATGAC GTACGCTGCC AACATCCTAC CTCACCATCC CGCTCGAGGC 
ACGTTTGAGG ACTTTACCTG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017450615.1
CDS	1351..2934
Translation	MELKTEEEEVGGVQPVSIQAFASSSTLHGLAHIFSYERLSLKRALWALCFLGSLAVLLCVCTERVQYYFCYHHVTKLDEVAASQLTFPAVTLCNLNEFRFSQVSKNDLYHAGELLALLNNRYEIPDTQMADEKQLEILQDKANFRSFKPKPFNMREFYDRAGHDIRDMLLSCHFRGEACSAEDFKVVFTRYGKCYTFNSGQDGRPRLKTMKGGTGNGLEIMLDIQQDEYLPVWGETDETSFEAGIKVQIHSQDEPPFIDQLGFGVAPGFQTFVSCQEQRQLIYLPSPWGTCNAVTMDSDFFDSYSITACRIDCETRYLVENCNCRMVHMPGDAPYCTPEQYKECADPALDFLVEKDQEYCVCEMPCNLTRYGKELSMVKIPSKASAKYLAKKFNKSEQYIGENILVLDIFFEVLNYETIEQKKAYEIAGLLGDIGGQMGLFIGASILTVLELFDYAYEVIKHRLCRRGKCQKEAKRSSADKGVALSLDDVKRHNPCESLRGHPAGMTYAANILPHHPARGTFEDFTC

Target ORF information:

RefSeq Version	XM_017595126.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus acid sensing ion channel subunit 1 (Asic1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017595126.1

ORF Insert Sequence:

ATGGAATTGA AGACCGAGGA GGAGGAGGTG GGTGGTGTCC AGCCGGTGAG CATCCAGGCT 
TTCGCCAGCA GCTCCACGCT GCATGGTCTT GCCCACATCT TCTCCTATGA GCGGCTGTCT 
CTGAAGCGGG CACTGTGGGC CCTGTGCTTC CTGGGTTCGC TGGCCGTCCT GCTGTGTGTG 
TGCACTGAGC GTGTGCAGTA CTACTTCTGC TATCACCACG TCACCAAGCT TGACGAAGTG 
GCTGCCTCCC AGCTCACCTT CCCTGCTGTC ACACTGTGCA ATCTCAATGA GTTCCGCTTT 
AGCCAAGTCT CCAAGAATGA CCTGTACCAT GCTGGGGAGC TGCTGGCCCT GCTCAACAAC 
AGGTATGAGA TCCCGGACAC ACAGATGGCT GATGAAAAGC AGCTAGAGAT ATTGCAGGAC 
AAGGCCAACT TCCGGAGCTT CAAGCCCAAG CCCTTCAACA TGCGTGAATT CTACGACAGA 
GCGGGGCACG ATATTCGAGA CATGCTGCTC TCGTGCCACT TCCGTGGGGA GGCCTGCAGC 
GCTGAAGATT TCAAAGTGGT CTTCACTCGG TATGGGAAGT GTTACACATT CAACTCGGGC 
CAAGATGGGC GGCCACGGCT GAAGACCATG AAAGGTGGGA CTGGCAATGG CCTGGAGATC 
ATGCTGGACA TTCAGCAAGA TGAATATTTG CCTGTGTGGG GAGAGACCGA CGAGACATCC 
TTCGAAGCAG GCATCAAAGT GCAGATCCAC AGTCAGGATG AACCCCCTTT CATCGACCAG 
CTGGGCTTTG GTGTGGCTCC AGGTTTCCAG ACGTTTGTGT CTTGCCAGGA GCAGAGGCAG 
CTCATCTACC TGCCCTCACC CTGGGGCACC TGCAATGCTG TTACCATGGA CTCGGATTTC 
TTCGACTCCT ACAGCATCAC TGCCTGCCGG ATTGATTGCG AGACGCGTTA CCTGGTGGAG 
AACTGCAACT GCCGTATGGT GCACATGCCA GGGGACGCCC CATACTGCAC TCCAGAGCAG 
TACAAGGAGT GTGCAGATCC TGCCCTGGAC TTCCTAGTGG AGAAAGACCA GGAATACTGC 
GTGTGTGAGA TGCCTTGCAA CCTGACCCGC TACGGCAAGG AGCTGTCCAT GGTCAAGATC 
CCAAGCAAAG CCTCCGCCAA GTACCTGGCC AAGAAGTTCA ACAAATCGGA GCAGTACATA 
GGGGAGAACA TTCTGGTGCT GGACATTTTC TTTGAAGTCC TCAACTATGA GACCATCGAG 
CAGAAAAAGG CCTATGAGAT CGCAGGGCTG TTGGGTGACA TCGGGGGCCA GATGGGGTTG 
TTCATCGGTG CCAGCATCCT CACCGTGCTG GAACTCTTTG ACTATGCCTA CGAGGTCATT 
AAGCACAGGC TGTGCAGACG TGGAAAGTGC CAGAAGGAGG CTAAGAGGAG CAGCGCAGAC 
AAGGGCGTGG CGCTCAGCCT GGATGACGTC AAAAGACACA ATCCCTGCGA GAGCCTCCGA 
GGACATCCTG CCGGGATGAC GTACGCTGCC AACATCCTAC CTCACCATCC CGCTCGAGGC 
ACGTTTGAGG ACTTTACCTG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Asic1 ( XM_017595125.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017450614.1 Rattus norvegicus acid sensing ion channel subunit 1 (Asic1), transcript variant X3, mRNA.

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CloneID	ORa62164
Clone ID Related Accession (Same CDS sequence)	XM_017595126.1 , XM_017595125.1 , XM_017595127.1
Accession Version	XM_017595125.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1584bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	acid-sensing ion channel 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATTGA AGACCGAGGA GGAGGAGGTG GGTGGTGTCC AGCCGGTGAG CATCCAGGCT 
TTCGCCAGCA GCTCCACGCT GCATGGTCTT GCCCACATCT TCTCCTATGA GCGGCTGTCT 
CTGAAGCGGG CACTGTGGGC CCTGTGCTTC CTGGGTTCGC TGGCCGTCCT GCTGTGTGTG 
TGCACTGAGC GTGTGCAGTA CTACTTCTGC TATCACCACG TCACCAAGCT TGACGAAGTG 
GCTGCCTCCC AGCTCACCTT CCCTGCTGTC ACACTGTGCA ATCTCAATGA GTTCCGCTTT 
AGCCAAGTCT CCAAGAATGA CCTGTACCAT GCTGGGGAGC TGCTGGCCCT GCTCAACAAC 
AGGTATGAGA TCCCGGACAC ACAGATGGCT GATGAAAAGC AGCTAGAGAT ATTGCAGGAC 
AAGGCCAACT TCCGGAGCTT CAAGCCCAAG CCCTTCAACA TGCGTGAATT CTACGACAGA 
GCGGGGCACG ATATTCGAGA CATGCTGCTC TCGTGCCACT TCCGTGGGGA GGCCTGCAGC 
GCTGAAGATT TCAAAGTGGT CTTCACTCGG TATGGGAAGT GTTACACATT CAACTCGGGC 
CAAGATGGGC GGCCACGGCT GAAGACCATG AAAGGTGGGA CTGGCAATGG CCTGGAGATC 
ATGCTGGACA TTCAGCAAGA TGAATATTTG CCTGTGTGGG GAGAGACCGA CGAGACATCC 
TTCGAAGCAG GCATCAAAGT GCAGATCCAC AGTCAGGATG AACCCCCTTT CATCGACCAG 
CTGGGCTTTG GTGTGGCTCC AGGTTTCCAG ACGTTTGTGT CTTGCCAGGA GCAGAGGCAG 
CTCATCTACC TGCCCTCACC CTGGGGCACC TGCAATGCTG TTACCATGGA CTCGGATTTC 
TTCGACTCCT ACAGCATCAC TGCCTGCCGG ATTGATTGCG AGACGCGTTA CCTGGTGGAG 
AACTGCAACT GCCGTATGGT GCACATGCCA GGGGACGCCC CATACTGCAC TCCAGAGCAG 
TACAAGGAGT GTGCAGATCC TGCCCTGGAC TTCCTAGTGG AGAAAGACCA GGAATACTGC 
GTGTGTGAGA TGCCTTGCAA CCTGACCCGC TACGGCAAGG AGCTGTCCAT GGTCAAGATC 
CCAAGCAAAG CCTCCGCCAA GTACCTGGCC AAGAAGTTCA ACAAATCGGA GCAGTACATA 
GGGGAGAACA TTCTGGTGCT GGACATTTTC TTTGAAGTCC TCAACTATGA GACCATCGAG 
CAGAAAAAGG CCTATGAGAT CGCAGGGCTG TTGGGTGACA TCGGGGGCCA GATGGGGTTG 
TTCATCGGTG CCAGCATCCT CACCGTGCTG GAACTCTTTG ACTATGCCTA CGAGGTCATT 
AAGCACAGGC TGTGCAGACG TGGAAAGTGC CAGAAGGAGG CTAAGAGGAG CAGCGCAGAC 
AAGGGCGTGG CGCTCAGCCT GGATGACGTC AAAAGACACA ATCCCTGCGA GAGCCTCCGA 
GGACATCCTG CCGGGATGAC GTACGCTGCC AACATCCTAC CTCACCATCC CGCTCGAGGC 
ACGTTTGAGG ACTTTACCTG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017450614.1
CDS	125..1708
Translation	MELKTEEEEVGGVQPVSIQAFASSSTLHGLAHIFSYERLSLKRALWALCFLGSLAVLLCVCTERVQYYFCYHHVTKLDEVAASQLTFPAVTLCNLNEFRFSQVSKNDLYHAGELLALLNNRYEIPDTQMADEKQLEILQDKANFRSFKPKPFNMREFYDRAGHDIRDMLLSCHFRGEACSAEDFKVVFTRYGKCYTFNSGQDGRPRLKTMKGGTGNGLEIMLDIQQDEYLPVWGETDETSFEAGIKVQIHSQDEPPFIDQLGFGVAPGFQTFVSCQEQRQLIYLPSPWGTCNAVTMDSDFFDSYSITACRIDCETRYLVENCNCRMVHMPGDAPYCTPEQYKECADPALDFLVEKDQEYCVCEMPCNLTRYGKELSMVKIPSKASAKYLAKKFNKSEQYIGENILVLDIFFEVLNYETIEQKKAYEIAGLLGDIGGQMGLFIGASILTVLELFDYAYEVIKHRLCRRGKCQKEAKRSSADKGVALSLDDVKRHNPCESLRGHPAGMTYAANILPHHPARGTFEDFTC

Target ORF information:

RefSeq Version	XM_017595125.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus acid sensing ion channel subunit 1 (Asic1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017595125.1

ORF Insert Sequence:

ATGGAATTGA AGACCGAGGA GGAGGAGGTG GGTGGTGTCC AGCCGGTGAG CATCCAGGCT 
TTCGCCAGCA GCTCCACGCT GCATGGTCTT GCCCACATCT TCTCCTATGA GCGGCTGTCT 
CTGAAGCGGG CACTGTGGGC CCTGTGCTTC CTGGGTTCGC TGGCCGTCCT GCTGTGTGTG 
TGCACTGAGC GTGTGCAGTA CTACTTCTGC TATCACCACG TCACCAAGCT TGACGAAGTG 
GCTGCCTCCC AGCTCACCTT CCCTGCTGTC ACACTGTGCA ATCTCAATGA GTTCCGCTTT 
AGCCAAGTCT CCAAGAATGA CCTGTACCAT GCTGGGGAGC TGCTGGCCCT GCTCAACAAC 
AGGTATGAGA TCCCGGACAC ACAGATGGCT GATGAAAAGC AGCTAGAGAT ATTGCAGGAC 
AAGGCCAACT TCCGGAGCTT CAAGCCCAAG CCCTTCAACA TGCGTGAATT CTACGACAGA 
GCGGGGCACG ATATTCGAGA CATGCTGCTC TCGTGCCACT TCCGTGGGGA GGCCTGCAGC 
GCTGAAGATT TCAAAGTGGT CTTCACTCGG TATGGGAAGT GTTACACATT CAACTCGGGC 
CAAGATGGGC GGCCACGGCT GAAGACCATG AAAGGTGGGA CTGGCAATGG CCTGGAGATC 
ATGCTGGACA TTCAGCAAGA TGAATATTTG CCTGTGTGGG GAGAGACCGA CGAGACATCC 
TTCGAAGCAG GCATCAAAGT GCAGATCCAC AGTCAGGATG AACCCCCTTT CATCGACCAG 
CTGGGCTTTG GTGTGGCTCC AGGTTTCCAG ACGTTTGTGT CTTGCCAGGA GCAGAGGCAG 
CTCATCTACC TGCCCTCACC CTGGGGCACC TGCAATGCTG TTACCATGGA CTCGGATTTC 
TTCGACTCCT ACAGCATCAC TGCCTGCCGG ATTGATTGCG AGACGCGTTA CCTGGTGGAG 
AACTGCAACT GCCGTATGGT GCACATGCCA GGGGACGCCC CATACTGCAC TCCAGAGCAG 
TACAAGGAGT GTGCAGATCC TGCCCTGGAC TTCCTAGTGG AGAAAGACCA GGAATACTGC 
GTGTGTGAGA TGCCTTGCAA CCTGACCCGC TACGGCAAGG AGCTGTCCAT GGTCAAGATC 
CCAAGCAAAG CCTCCGCCAA GTACCTGGCC AAGAAGTTCA ACAAATCGGA GCAGTACATA 
GGGGAGAACA TTCTGGTGCT GGACATTTTC TTTGAAGTCC TCAACTATGA GACCATCGAG 
CAGAAAAAGG CCTATGAGAT CGCAGGGCTG TTGGGTGACA TCGGGGGCCA GATGGGGTTG 
TTCATCGGTG CCAGCATCCT CACCGTGCTG GAACTCTTTG ACTATGCCTA CGAGGTCATT 
AAGCACAGGC TGTGCAGACG TGGAAAGTGC CAGAAGGAGG CTAAGAGGAG CAGCGCAGAC 
AAGGGCGTGG CGCTCAGCCT GGATGACGTC AAAAGACACA ATCCCTGCGA GAGCCTCCGA 
GGACATCCTG CCGGGATGAC GTACGCTGCC AACATCCTAC CTCACCATCC CGCTCGAGGC 
ACGTTTGAGG ACTTTACCTG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Asic1 ( XM_017595127.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017450616.1 Rattus norvegicus acid sensing ion channel subunit 1 (Asic1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ORa62164
Clone ID Related Accession (Same CDS sequence)	XM_017595126.1 , XM_017595125.1 , XM_017595127.1
Accession Version	XM_017595127.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1584bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	acid-sensing ion channel 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATTGA AGACCGAGGA GGAGGAGGTG GGTGGTGTCC AGCCGGTGAG CATCCAGGCT 
TTCGCCAGCA GCTCCACGCT GCATGGTCTT GCCCACATCT TCTCCTATGA GCGGCTGTCT 
CTGAAGCGGG CACTGTGGGC CCTGTGCTTC CTGGGTTCGC TGGCCGTCCT GCTGTGTGTG 
TGCACTGAGC GTGTGCAGTA CTACTTCTGC TATCACCACG TCACCAAGCT TGACGAAGTG 
GCTGCCTCCC AGCTCACCTT CCCTGCTGTC ACACTGTGCA ATCTCAATGA GTTCCGCTTT 
AGCCAAGTCT CCAAGAATGA CCTGTACCAT GCTGGGGAGC TGCTGGCCCT GCTCAACAAC 
AGGTATGAGA TCCCGGACAC ACAGATGGCT GATGAAAAGC AGCTAGAGAT ATTGCAGGAC 
AAGGCCAACT TCCGGAGCTT CAAGCCCAAG CCCTTCAACA TGCGTGAATT CTACGACAGA 
GCGGGGCACG ATATTCGAGA CATGCTGCTC TCGTGCCACT TCCGTGGGGA GGCCTGCAGC 
GCTGAAGATT TCAAAGTGGT CTTCACTCGG TATGGGAAGT GTTACACATT CAACTCGGGC 
CAAGATGGGC GGCCACGGCT GAAGACCATG AAAGGTGGGA CTGGCAATGG CCTGGAGATC 
ATGCTGGACA TTCAGCAAGA TGAATATTTG CCTGTGTGGG GAGAGACCGA CGAGACATCC 
TTCGAAGCAG GCATCAAAGT GCAGATCCAC AGTCAGGATG AACCCCCTTT CATCGACCAG 
CTGGGCTTTG GTGTGGCTCC AGGTTTCCAG ACGTTTGTGT CTTGCCAGGA GCAGAGGCAG 
CTCATCTACC TGCCCTCACC CTGGGGCACC TGCAATGCTG TTACCATGGA CTCGGATTTC 
TTCGACTCCT ACAGCATCAC TGCCTGCCGG ATTGATTGCG AGACGCGTTA CCTGGTGGAG 
AACTGCAACT GCCGTATGGT GCACATGCCA GGGGACGCCC CATACTGCAC TCCAGAGCAG 
TACAAGGAGT GTGCAGATCC TGCCCTGGAC TTCCTAGTGG AGAAAGACCA GGAATACTGC 
GTGTGTGAGA TGCCTTGCAA CCTGACCCGC TACGGCAAGG AGCTGTCCAT GGTCAAGATC 
CCAAGCAAAG CCTCCGCCAA GTACCTGGCC AAGAAGTTCA ACAAATCGGA GCAGTACATA 
GGGGAGAACA TTCTGGTGCT GGACATTTTC TTTGAAGTCC TCAACTATGA GACCATCGAG 
CAGAAAAAGG CCTATGAGAT CGCAGGGCTG TTGGGTGACA TCGGGGGCCA GATGGGGTTG 
TTCATCGGTG CCAGCATCCT CACCGTGCTG GAACTCTTTG ACTATGCCTA CGAGGTCATT 
AAGCACAGGC TGTGCAGACG TGGAAAGTGC CAGAAGGAGG CTAAGAGGAG CAGCGCAGAC 
AAGGGCGTGG CGCTCAGCCT GGATGACGTC AAAAGACACA ATCCCTGCGA GAGCCTCCGA 
GGACATCCTG CCGGGATGAC GTACGCTGCC AACATCCTAC CTCACCATCC CGCTCGAGGC 
ACGTTTGAGG ACTTTACCTG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017450616.1
CDS	137..1720
Translation	MELKTEEEEVGGVQPVSIQAFASSSTLHGLAHIFSYERLSLKRALWALCFLGSLAVLLCVCTERVQYYFCYHHVTKLDEVAASQLTFPAVTLCNLNEFRFSQVSKNDLYHAGELLALLNNRYEIPDTQMADEKQLEILQDKANFRSFKPKPFNMREFYDRAGHDIRDMLLSCHFRGEACSAEDFKVVFTRYGKCYTFNSGQDGRPRLKTMKGGTGNGLEIMLDIQQDEYLPVWGETDETSFEAGIKVQIHSQDEPPFIDQLGFGVAPGFQTFVSCQEQRQLIYLPSPWGTCNAVTMDSDFFDSYSITACRIDCETRYLVENCNCRMVHMPGDAPYCTPEQYKECADPALDFLVEKDQEYCVCEMPCNLTRYGKELSMVKIPSKASAKYLAKKFNKSEQYIGENILVLDIFFEVLNYETIEQKKAYEIAGLLGDIGGQMGLFIGASILTVLELFDYAYEVIKHRLCRRGKCQKEAKRSSADKGVALSLDDVKRHNPCESLRGHPAGMTYAANILPHHPARGTFEDFTC

Target ORF information:

RefSeq Version	XM_017595127.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus acid sensing ion channel subunit 1 (Asic1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017595127.1

ORF Insert Sequence:

ATGGAATTGA AGACCGAGGA GGAGGAGGTG GGTGGTGTCC AGCCGGTGAG CATCCAGGCT 
TTCGCCAGCA GCTCCACGCT GCATGGTCTT GCCCACATCT TCTCCTATGA GCGGCTGTCT 
CTGAAGCGGG CACTGTGGGC CCTGTGCTTC CTGGGTTCGC TGGCCGTCCT GCTGTGTGTG 
TGCACTGAGC GTGTGCAGTA CTACTTCTGC TATCACCACG TCACCAAGCT TGACGAAGTG 
GCTGCCTCCC AGCTCACCTT CCCTGCTGTC ACACTGTGCA ATCTCAATGA GTTCCGCTTT 
AGCCAAGTCT CCAAGAATGA CCTGTACCAT GCTGGGGAGC TGCTGGCCCT GCTCAACAAC 
AGGTATGAGA TCCCGGACAC ACAGATGGCT GATGAAAAGC AGCTAGAGAT ATTGCAGGAC 
AAGGCCAACT TCCGGAGCTT CAAGCCCAAG CCCTTCAACA TGCGTGAATT CTACGACAGA 
GCGGGGCACG ATATTCGAGA CATGCTGCTC TCGTGCCACT TCCGTGGGGA GGCCTGCAGC 
GCTGAAGATT TCAAAGTGGT CTTCACTCGG TATGGGAAGT GTTACACATT CAACTCGGGC 
CAAGATGGGC GGCCACGGCT GAAGACCATG AAAGGTGGGA CTGGCAATGG CCTGGAGATC 
ATGCTGGACA TTCAGCAAGA TGAATATTTG CCTGTGTGGG GAGAGACCGA CGAGACATCC 
TTCGAAGCAG GCATCAAAGT GCAGATCCAC AGTCAGGATG AACCCCCTTT CATCGACCAG 
CTGGGCTTTG GTGTGGCTCC AGGTTTCCAG ACGTTTGTGT CTTGCCAGGA GCAGAGGCAG 
CTCATCTACC TGCCCTCACC CTGGGGCACC TGCAATGCTG TTACCATGGA CTCGGATTTC 
TTCGACTCCT ACAGCATCAC TGCCTGCCGG ATTGATTGCG AGACGCGTTA CCTGGTGGAG 
AACTGCAACT GCCGTATGGT GCACATGCCA GGGGACGCCC CATACTGCAC TCCAGAGCAG 
TACAAGGAGT GTGCAGATCC TGCCCTGGAC TTCCTAGTGG AGAAAGACCA GGAATACTGC 
GTGTGTGAGA TGCCTTGCAA CCTGACCCGC TACGGCAAGG AGCTGTCCAT GGTCAAGATC 
CCAAGCAAAG CCTCCGCCAA GTACCTGGCC AAGAAGTTCA ACAAATCGGA GCAGTACATA 
GGGGAGAACA TTCTGGTGCT GGACATTTTC TTTGAAGTCC TCAACTATGA GACCATCGAG 
CAGAAAAAGG CCTATGAGAT CGCAGGGCTG TTGGGTGACA TCGGGGGCCA GATGGGGTTG 
TTCATCGGTG CCAGCATCCT CACCGTGCTG GAACTCTTTG ACTATGCCTA CGAGGTCATT 
AAGCACAGGC TGTGCAGACG TGGAAAGTGC CAGAAGGAGG CTAAGAGGAG CAGCGCAGAC 
AAGGGCGTGG CGCTCAGCCT GGATGACGTC AAAAGACACA ATCCCTGCGA GAGCCTCCGA 
GGACATCCTG CCGGGATGAC GTACGCTGCC AACATCCTAC CTCACCATCC CGCTCGAGGC 
ACGTTTGAGG ACTTTACCTG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Inhibition of acid‑sensing ion channel 1a attenuates acid‑induced activation of autophagy via a calcium signaling pathway in articular chondrocytes.
International journal of molecular medicine43(4)1778-1788(2019 Apr)
Gao WF,Xu YY,Ge JF,Chen FH

The role of Ca
Laboratory investigation; a journal of technical methods and pathology99(4)499-513(2019 04)
Wu X,Ren G,Zhou R,Ge J,Chen FH

Effects of autophagy on acid-sensing ion channel 1a-mediated apoptosis in rat articular chondrocytes.
Molecular and cellular biochemistry443(1-2)181-191(2018 Jun)
Xie YY,Li Y,Zhou RP,Dai BB,Qian YJ,Wu XS,Ge JF,Hu W,Chen FH

ASIC1a contributes to the symptom of pain in a rat model of chronic prostatitis.
Asian journal of andrology20(3)300-305(2018 May-Jun)
Fan S,Hao ZY,Zhang L,Zhou J,Zhang YF,Tai S,Zhang XS,Liang CZ

Dual actions of Psalmotoxin at ASIC1a and ASIC2a heteromeric channels (ASIC1a/2a).
Scientific reports8(1)7179(2018 05)
Liu Y,Hagan R,Schoellerman J

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Asic1 cDNA ORF clone, Rattus norvegicus(Norway rat)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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