Slc12a6 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Slc12a6
Entrez Gene ID	691209
Full Name	solute carrier family 12, member 6
Synonyms	Kcc3
General protein information	Preferred Names solute carrier family 12, member 6 Names solute carrier family 12 member 6 furosemide-sensitive KCl cotransporter 3
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q35

mRNA and Protein(s)

mRNA	Protein	Name
NM_001109630.1	NP_001103100.1	solute carrier family 12 member 6
XM_006234710.3	XP_006234772.1	solute carrier family 12 member 6 isoform X1
XM_006234711.3	XP_006234773.1	solute carrier family 12 member 6 isoform X2

Pathways from BioSystems

Homologies

Bos taurus (cattle)	SLC12A6	NP_001180045.1
Mus musculus (house mouse)	Slc12a6	NP_598410.2
Xenopus tropicalis (tropical clawed frog)	slc12a6	NP_001072576.1
Macaca mulatta (Rhesus monkey)	SLC12A6	XP_002804750.1
Danio rerio (zebrafish)	slc12a7b	XP_696060.6
Homo sapiens (human)	SLC12A6	NP_598408.1
Pan troglodytes (chimpanzee)	SLC12A6	XP_001173480.1
Rattus norvegicus (Norway rat)	Slc12a6	NP_001103100.1
Canis lupus familiaris (dog)	SLC12A6	XP_005638240.1

Gene Ontology
Bibliography

Related articles in PubMed

The WNK-regulated SPAK/OSR1 kinases directly phosphorylate and inhibit the K+-Cl- co-transporters.
de Los Heros P, Alessi DR, Gourlay R, Campbell DG, Deak M, Macartney TJ, Kahle KT, Zhang J
The Biochemical journal458(3)559-73(2014 Mar)

Molecular evidence for a role for K(+)-Cl(-) cotransporters in the kidney.
Melo Z, Cruz-Rangel S, Bautista R, Vázquez N, Castañeda-Bueno M, Mount DB, Pasantes-Morales H, Mercado A, Gamba G
American journal of physiology. Renal physiology305(10)F1402-11(2013 Nov)

Role of cholesterol in functional association between K(+)-Cl(-) cotransporter-3a and Na+,K(+)-ATPase.
Fujita K, Fujii T, Shimizu T, Takeguchi N, Sakai H
Biochemical and biophysical research communications424(1)136-40(2012 Jul)

High-grade glioma motility reduced by genetic knockdown of KCC3.
Gagnon KB
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology30(2)466-76(2012)

The NH(2)-terminus of K(+)-Cl(-) cotransporter 3a is essential for up-regulation of Na(+),K(+)-ATPase activity.
Fujii T, Fujita K, Shimizu T, Takeguchi N, Sakai H
Biochemical and biophysical research communications399(4)683-7(2010 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Slc12a6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Slc12a6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa10578	NM_001109630.1 Latest version!	Rattus norvegicus solute carrier family 12, member 6 (Slc12a6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa26189	XM_006234710.3 Latest version!	Rattus norvegicus solute carrier family 12, member 6 (Slc12a6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa26190	XM_006234711.3 Latest version!	Rattus norvegicus solute carrier family 12, member 6 (Slc12a6), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Slc12a6 ( NM_001109630.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001103100.1 Rattus norvegicus solute carrier family 12, member 6 (Slc12a6), mRNA.

Price & Availability↑

CloneID	ORa10578
Clone ID Related Accession (Same CDS sequence)	NM_001109630.1
Accession Version	NM_001109630.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3453bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1527350400000
Organism	Rattus norvegicus(Norway rat)
Product	solute carrier family 12 member 6
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473949.1. On or before Oct 4, 2007 this sequence version replaced XM_001066528.1, XM_001066596.1, XM_001066706.1, XM_001066756.1, XM_001066648.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGCATCCTC CAGAAACCAC CACCAAGATG TCTTCAGTTC GGTTCATGGT GACACCAACA 
AAGATTGATG ACATTCCAGG TTTATCAGAC ACCAGTCCGG ACCTCAGCTC TCGATCTAGT 
TCTCGAGTAA GATTTAGCTC CCGAGAAAGT GTGCCAGAAA CAAGCCGTAG TGAGCCTATG 
AGTGAACTGT CTGGGGCCAC CACTTCTCTG GCAACTGTTG CCCTAGATCC TTCCAGTGAC 
CGGACTTCTA ACCCCCAGGA TGTTACCGAG GACCCGAATC AGAACTCCAT CACAGGGGAG 
CACAGCCAGC TGTTAGATGA TGGACATAAG AAAGCTCGAA ATGCTTATCT CAATAATTCC 
AACTATGAAG AAGGAGACGA ATATTTTGAT AAAAATCTGG CACTCTTTGA GGAAGAAATG 
GACACCAGAC CAAAGGTGTC TTCTCTCCTC AACCGCATGG CCAACTATAC AAATCTGACA 
CAAGGAGCAA AGGAACATGA AGAGGCAGAG AACATCACCG AAGGGAAAAA GAAGCCTACC 
AAGAGCCCCC AAATGGGTAC TTTCATGGGT GTCTACCTCC CATGTCTACA AAATATTTTT 
GGAGTGATCC TCTTTCTGCG TCTTACCTGG GTAGTGGGCA CAGCTGGAGT CCTTCAGGCC 
TTTGCCATTG TCCTTATCTG CTGCTGCTGT ACAATGTTAA CTGCCATCTC CATGAGTGCC 
ATCGCCACTA ACGGAGTGGT GCCAGCTGGA GGCTCATACT TCATGATTTC CCGGGCCCTG 
GGCCCAGAGT TTGGCGGGGC TGTGGGCCTC TGCTTTTATC TTGGCACCAC ATTTGCAGCA 
GCCATGTATA TTCTTGGTGC CATTGAAATC TTTCTGGTGT ATATTGTCCC CCGAGCTGCC 
ATCTTTCGGA GCGATGATGC ACTCAAGGAG TCAGCAGCCA TGCTGAACAA CATGCGTGTC 
TATGGTACAG CCTTCCTGGT CCTCATGGTC TTGGTGGTGT TCATCGGCGT ACGCTATGTG 
AATAAGTTTG CCTCACTTTT CCTGGCCTGT GTAATTGTGT CGATCTTGGC TATCTATGCT 
GGTGCCATCA AGTCTTCCTT TGCTCCACCA CACTTCCCGG TCTGTATGCT GGGCAACCGC 
ACCCTGTCAT CAAGACACCT TGACATTTGC TCTAAGACCA AGGAAGTTAA CAACATGACA 
ATACCATCAA AGTTATGGGG ATTCTTCTGC AACTCAAGTC AGTTTTTTAA TGCCACCTGT 
GATGAGTACT TTGTTCACAA TAATGTCACC TCAATCCAGG GCATTCCAGG GTTGGCTAGT 
GGCATCATTA CTGAAAACCT TTGGAGTAAT TATTTACCAA AAGGTGAGAT AATTGAAAAG 
CCATCAGCCA AGTCATCTGA CGTTTTGGGC AACTTAAACC ATGAATATGT TCTTGCTGAT 
ATTACCACCT CCTTCACTCT TCTGGTGGGG ATCTTCTTTC CTTCTGTCAC AGGTATCATG 
GCTGGGTCAA ACAGATCTGG AGATCTGAAA GATGCTCAAA AGTCTATCCC CATTGGGACC 
ATCCTTGCCA TCCTGACCAC ATCCTTCGTG TATTTAAGCA ATGTTGTCCT TTTTGGTGCA 
TGTATCGAAG GAGTTGTCCT CAGAGACAAA TTTGGGGATG CTGTAAAAGG GAATTTGGTT 
GTAGGCACCT TATCCTGGCC ATCCCCATGG GTGATTGTTA TTGGCTCCTT CTTTTCAACG 
TGTGGAGCTG GACTTCAGAG CCTCACAGGT GCACCTCGGC TGCTGCAGGC TATTGCCAAG 
GACAACATCA TACCTTTCCT TAGGGTTTTT GGACACAGTA AAGCTAATGG GGAACCTACC 
TGGGCCTTAC TTCTAACTGC TGCCATAGCA GAGCTGGGAA TTCTCATCGC CTCCCTGGAT 
CTCGTGGCCC CAATTCTTTC CATGTTTTTT CTCATGTGTT ACCTCTTTGT GAACTTGGCT 
TGTGCCTTGC AAACATTACT GCGAACCCCC CACTGGAGGC CTCGATTCCG CTACTATCAC 
TGGGCCCTCT CTTTCATGGG AATGAGTATC TGTCTAGCTC TGATGTTCAT TTCTTCTTGG 
TATTATGCCA TTGTAGCTAT GGTAATAGCT GGCATGATCT ACAAGTACAT TGAATACCAA 
GGGGCTGAAA AAGAATGGGG CGATGGTATC CGTGGGCTGT CACTCAGTGC AGCCCGCTTC 
GCTTTGCTCC GCCTAGAGGA AGGCCCTCCT CACACTAAAA ACTGGAGGCC TCAGCTGCTT 
GTACTGCTAA AGCTGGATGA AGACTTACAC GTCAAGCACC CTCGCCTCCT CACCTTTGCC 
TCACAGCTCA AGGCAGGAAA GGGACTCACC ATTGTGGGCT CTGTCATCGT GGGGAACTTC 
CTGGAGAACT ATGGTGACGC TCTAGCAGCA GAGCAGACCA TTAAGCACCT CATGGAGGCA 
GAAAAGGTAA AAGGGTTCTG CCAGCTGGTA GTGGCTGCCA AGCTGAAAGA GGGCATTTCA 
CACCTCATCC AGTCCTGTGG CCTTGGAGGC ATGAAGCACA ACACAGTGGT GATGGGCTGG 
CCCAATGGCT GGCGTCAGAG TGAAGATGCT CGTGCTTGGA AGACTTTCAT TGGCACAGTT 
CGAGTGACAA CTGCTGCCCA TCTAGCCCTG TTAGTGGCTA AAAATGTCTC CTTCTTTCCC 
AGCAATGTGG AACAATTTTC TGAGGGCAAC ATTGATGTGT GGTGGATTGT GCATGATGGG 
GGCATGCTCA TGCTATTACC ATTCCTACTG AAACAGCACA AGGTTTGGCG AAAATGCAGC 
ATACGGATCT TCACAGTAGC CCAACTAGAA GACAACAGTA TCCAGATGAA GAAGGACCTG 
GCCACCTTCC TGTACCATCT GCGCATCGAG GCCGAAGTGG AAGTGGTGGA GATGCATGAC 
AGTGACATAT CTGCCTATAC TTATGAGCGC ACCCTGATGA TGGAGCAGAG GTCCCAGATG 
CTTCGGCACA TGCGGCTGTC CAAAACAGAA CGAGATAGAG AGGCACAACT GGTGAAAGAT 
CGAAACTCAA TGCTTCGCTT AACTAGCATT GGCTCTGACG AGGATGAAGA AACAGAAACT 
TACCAAGAGA AGGTGCACAT GACTTGGACC AAGGATAAAT ACATGGCATC CCGGGGGCAA 
AAGGTCAAAT CAATGGAAGG ATTCCAAGAC CTACTTAATA TGCGTCCGGA CCAGTCCAAC 
GTGAGACGGA TGCATACAGC AGTGAAGCTC AATGAAGTTA TAGTTAACAA GTCCCACGAA 
GCAAAGCTGG TTTTGTTGAA TATGCCAGGA CCACCCCGAA ACCCTGAAGG TGATGAGAAC 
TACATGGAAT TTCTAGAAGT GCTCACTGAG GGATTAGAGC GAGTCCTCCT CGTCCGGGGT 
GGTGGCAGTG AAGTCATCAC CATATACTCA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001103100.1
CDS	4..3456
Translation	MHPPETTTKMSSVRFMVTPTKIDDIPGLSDTSPDLSSRSSSRVRFSSRESVPETSRSEPMSELSGATTSLATVALDPSSDRTSNPQDVTEDPNQNSITGEHSQLLDDGHKKARNAYLNNSNYEEGDEYFDKNLALFEEEMDTRPKVSSLLNRMANYTNLTQGAKEHEEAENITEGKKKPTKSPQMGTFMGVYLPCLQNIFGVILFLRLTWVVGTAGVLQAFAIVLICCCCTMLTAISMSAIATNGVVPAGGSYFMISRALGPEFGGAVGLCFYLGTTFAAAMYILGAIEIFLVYIVPRAAIFRSDDALKESAAMLNNMRVYGTAFLVLMVLVVFIGVRYVNKFASLFLACVIVSILAIYAGAIKSSFAPPHFPVCMLGNRTLSSRHLDICSKTKEVNNMTIPSKLWGFFCNSSQFFNATCDEYFVHNNVTSIQGIPGLASGIITENLWSNYLPKGEIIEKPSAKSSDVLGNLNHEYVLADITTSFTLLVGIFFPSVTGIMAGSNRSGDLKDAQKSIPIGTILAILTTSFVYLSNVVLFGACIEGVVLRDKFGDAVKGNLVVGTLSWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAIAKDNIIPFLRVFGHSKANGEPTWALLLTAAIAELGILIASLDLVAPILSMFFLMCYLFVNLACALQTLLRTPHWRPRFRYYHWALSFMGMSICLALMFISSWYYAIVAMVIAGMIYKYIEYQGAEKEWGDGIRGLSLSAARFALLRLEEGPPHTKNWRPQLLVLLKLDEDLHVKHPRLLTFASQLKAGKGLTIVGSVIVGNFLENYGDALAAEQTIKHLMEAEKVKGFCQLVVAAKLKEGISHLIQSCGLGGMKHNTVVMGWPNGWRQSEDARAWKTFIGTVRVTTAAHLALLVAKNVSFFPSNVEQFSEGNIDVWWIVHDGGMLMLLPFLLKQHKVWRKCSIRIFTVAQLEDNSIQMKKDLATFLYHLRIEAEVEVVEMHDSDISAYTYERTLMMEQRSQMLRHMRLSKTERDREAQLVKDRNSMLRLTSIGSDEDEETETYQEKVHMTWTKDKYMASRGQKVKSMEGFQDLLNMRPDQSNVRRMHTAVKLNEVIVNKSHEAKLVLLNMPGPPRNPEGDENYMEFLEVLTEGLERVLLVRGGGSEVITIYS

Target ORF information:

RefSeq Version	NM_001109630.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus solute carrier family 12, member 6 (Slc12a6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001109630.1

ORF Insert Sequence:

ATGCATCCTC CAGAAACCAC CACCAAGATG TCTTCAGTTC GGTTCATGGT GACACCAACA 
AAGATTGATG ACATTCCAGG TTTATCAGAC ACCAGTCCGG ACCTCAGCTC TCGATCTAGT 
TCTCGAGTAA GATTTAGCTC CCGAGAAAGT GTGCCAGAAA CAAGCCGTAG TGAGCCTATG 
AGTGAACTGT CTGGGGCCAC CACTTCTCTG GCAACTGTTG CCCTAGATCC TTCCAGTGAC 
CGGACTTCTA ACCCCCAGGA TGTTACCGAG GACCCGAATC AGAACTCCAT CACAGGGGAG 
CACAGCCAGC TGTTAGATGA TGGACATAAG AAAGCTCGAA ATGCTTATCT CAATAATTCC 
AACTATGAAG AAGGAGACGA ATATTTTGAT AAAAATCTGG CACTCTTTGA GGAAGAAATG 
GACACCAGAC CAAAGGTGTC TTCTCTCCTC AACCGCATGG CCAACTATAC AAATCTGACA 
CAAGGAGCAA AGGAACATGA AGAGGCAGAG AACATCACCG AAGGGAAAAA GAAGCCTACC 
AAGAGCCCCC AAATGGGTAC TTTCATGGGT GTCTACCTCC CATGTCTACA AAATATTTTT 
GGAGTGATCC TCTTTCTGCG TCTTACCTGG GTAGTGGGCA CAGCTGGAGT CCTTCAGGCC 
TTTGCCATTG TCCTTATCTG CTGCTGCTGT ACAATGTTAA CTGCCATCTC CATGAGTGCC 
ATCGCCACTA ACGGAGTGGT GCCAGCTGGA GGCTCATACT TCATGATTTC CCGGGCCCTG 
GGCCCAGAGT TTGGCGGGGC TGTGGGCCTC TGCTTTTATC TTGGCACCAC ATTTGCAGCA 
GCCATGTATA TTCTTGGTGC CATTGAAATC TTTCTGGTGT ATATTGTCCC CCGAGCTGCC 
ATCTTTCGGA GCGATGATGC ACTCAAGGAG TCAGCAGCCA TGCTGAACAA CATGCGTGTC 
TATGGTACAG CCTTCCTGGT CCTCATGGTC TTGGTGGTGT TCATCGGCGT ACGCTATGTG 
AATAAGTTTG CCTCACTTTT CCTGGCCTGT GTAATTGTGT CGATCTTGGC TATCTATGCT 
GGTGCCATCA AGTCTTCCTT TGCTCCACCA CACTTCCCGG TCTGTATGCT GGGCAACCGC 
ACCCTGTCAT CAAGACACCT TGACATTTGC TCTAAGACCA AGGAAGTTAA CAACATGACA 
ATACCATCAA AGTTATGGGG ATTCTTCTGC AACTCAAGTC AGTTTTTTAA TGCCACCTGT 
GATGAGTACT TTGTTCACAA TAATGTCACC TCAATCCAGG GCATTCCAGG GTTGGCTAGT 
GGCATCATTA CTGAAAACCT TTGGAGTAAT TATTTACCAA AAGGTGAGAT AATTGAAAAG 
CCATCAGCCA AGTCATCTGA CGTTTTGGGC AACTTAAACC ATGAATATGT TCTTGCTGAT 
ATTACCACCT CCTTCACTCT TCTGGTGGGG ATCTTCTTTC CTTCTGTCAC AGGTATCATG 
GCTGGGTCAA ACAGATCTGG AGATCTGAAA GATGCTCAAA AGTCTATCCC CATTGGGACC 
ATCCTTGCCA TCCTGACCAC ATCCTTCGTG TATTTAAGCA ATGTTGTCCT TTTTGGTGCA 
TGTATCGAAG GAGTTGTCCT CAGAGACAAA TTTGGGGATG CTGTAAAAGG GAATTTGGTT 
GTAGGCACCT TATCCTGGCC ATCCCCATGG GTGATTGTTA TTGGCTCCTT CTTTTCAACG 
TGTGGAGCTG GACTTCAGAG CCTCACAGGT GCACCTCGGC TGCTGCAGGC TATTGCCAAG 
GACAACATCA TACCTTTCCT TAGGGTTTTT GGACACAGTA AAGCTAATGG GGAACCTACC 
TGGGCCTTAC TTCTAACTGC TGCCATAGCA GAGCTGGGAA TTCTCATCGC CTCCCTGGAT 
CTCGTGGCCC CAATTCTTTC CATGTTTTTT CTCATGTGTT ACCTCTTTGT GAACTTGGCT 
TGTGCCTTGC AAACATTACT GCGAACCCCC CACTGGAGGC CTCGATTCCG CTACTATCAC 
TGGGCCCTCT CTTTCATGGG AATGAGTATC TGTCTAGCTC TGATGTTCAT TTCTTCTTGG 
TATTATGCCA TTGTAGCTAT GGTAATAGCT GGCATGATCT ACAAGTACAT TGAATACCAA 
GGGGCTGAAA AAGAATGGGG CGATGGTATC CGTGGGCTGT CACTCAGTGC AGCCCGCTTC 
GCTTTGCTCC GCCTAGAGGA AGGCCCTCCT CACACTAAAA ACTGGAGGCC TCAGCTGCTT 
GTACTGCTAA AGCTGGATGA AGACTTACAC GTCAAGCACC CTCGCCTCCT CACCTTTGCC 
TCACAGCTCA AGGCAGGAAA GGGACTCACC ATTGTGGGCT CTGTCATCGT GGGGAACTTC 
CTGGAGAACT ATGGTGACGC TCTAGCAGCA GAGCAGACCA TTAAGCACCT CATGGAGGCA 
GAAAAGGTAA AAGGGTTCTG CCAGCTGGTA GTGGCTGCCA AGCTGAAAGA GGGCATTTCA 
CACCTCATCC AGTCCTGTGG CCTTGGAGGC ATGAAGCACA ACACAGTGGT GATGGGCTGG 
CCCAATGGCT GGCGTCAGAG TGAAGATGCT CGTGCTTGGA AGACTTTCAT TGGCACAGTT 
CGAGTGACAA CTGCTGCCCA TCTAGCCCTG TTAGTGGCTA AAAATGTCTC CTTCTTTCCC 
AGCAATGTGG AACAATTTTC TGAGGGCAAC ATTGATGTGT GGTGGATTGT GCATGATGGG 
GGCATGCTCA TGCTATTACC ATTCCTACTG AAACAGCACA AGGTTTGGCG AAAATGCAGC 
ATACGGATCT TCACAGTAGC CCAACTAGAA GACAACAGTA TCCAGATGAA GAAGGACCTG 
GCCACCTTCC TGTACCATCT GCGCATCGAG GCCGAAGTGG AAGTGGTGGA GATGCATGAC 
AGTGACATAT CTGCCTATAC TTATGAGCGC ACCCTGATGA TGGAGCAGAG GTCCCAGATG 
CTTCGGCACA TGCGGCTGTC CAAAACAGAA CGAGATAGAG AGGCACAACT GGTGAAAGAT 
CGAAACTCAA TGCTTCGCTT AACTAGCATT GGCTCTGACG AGGATGAAGA AACAGAAACT 
TACCAAGAGA AGGTGCACAT GACTTGGACC AAGGATAAAT ACATGGCATC CCGGGGGCAA 
AAGGTCAAAT CAATGGAAGG ATTCCAAGAC CTACTTAATA TGCGTCCGGA CCAGTCCAAC 
GTGAGACGGA TGCATACAGC AGTGAAGCTC AATGAAGTTA TAGTTAACAA GTCCCACGAA 
GCAAAGCTGG TTTTGTTGAA TATGCCAGGA CCACCCCGAA ACCCTGAAGG TGATGAGAAC 
TACATGGAAT TTCTAGAAGT GCTCACTGAG GGATTAGAGC GAGTCCTCCT CGTCCGGGGT 
GGTGGCAGTG AAGTCATCAC CATATACTCA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slc12a6 ( XM_006234710.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006234772.1 Rattus norvegicus solute carrier family 12, member 6 (Slc12a6), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa26189
Clone ID Related Accession (Same CDS sequence)	XM_006234710.3
Accession Version	XM_006234710.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3408bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	solute carrier family 12 member 6 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006234710.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCCTC CAGAAACCAC CACCAAGATG TCTTCAGTTC GGTTCATGGT GACACCAACA 
AAGATTGATG ACATTCCAGG TTTATCAGAC ACCAGTCCGG ACCTCAGCTC TCGATCTAGT 
TCTCGAGTAA GATTTAGCTC CCGAGAAAGT GTGCCAGAAA CAAGCCGTAG TGAGCCTATG 
AGTGAACTGT CTGGGGCCAC CACTTCTCTG GCAACTGTTG CCCTAGATCC TTCCAGTGAC 
CGGACTTCTA ACCCCCAGGA TGTTACCGAG GATGATGGAC ATAAGAAAGC TCGAAATGCT 
TATCTCAATA ATTCCAACTA TGAAGAAGGA GACGAATATT TTGATAAAAA TCTGGCACTC 
TTTGAGGAAG AAATGGACAC CAGACCAAAG GTGTCTTCTC TCCTCAACCG CATGGCCAAC 
TATACAAATC TGACACAAGG AGCAAAGGAA CATGAAGAGG CAGAGAACAT CACCGAAGGG 
AAAAAGAAGC CTACCAAGAG CCCCCAAATG GGTACTTTCA TGGGTGTCTA CCTCCCATGT 
CTACAAAATA TTTTTGGAGT GATCCTCTTT CTGCGTCTTA CCTGGGTAGT GGGCACAGCT 
GGAGTCCTTC AGGCCTTTGC CATTGTCCTT ATCTGCTGCT GCTGTACAAT GTTAACTGCC 
ATCTCCATGA GTGCCATCGC CACTAACGGA GTGGTGCCAG CTGGAGGCTC ATACTTCATG 
ATTTCCCGGG CCCTGGGCCC AGAGTTTGGC GGGGCTGTGG GCCTCTGCTT TTATCTTGGC 
ACCACATTTG CAGCAGCCAT GTATATTCTT GGTGCCATTG AAATCTTTCT GGTGTATATT 
GTCCCCCGAG CTGCCATCTT TCGGAGCGAT GATGCACTCA AGGAGTCAGC AGCCATGCTG 
AACAACATGC GTGTCTATGG TACAGCCTTC CTGGTCCTCA TGGTCTTGGT GGTGTTCATC 
GGCGTACGCT ATGTGAATAA GTTTGCCTCA CTTTTCCTGG CCTGTGTAAT TGTGTCGATC 
TTGGCTATCT ATGCTGGTGC CATCAAGTCT TCCTTTGCTC CACCACACTT CCCGGTCTGT 
ATGCTGGGCA ACCGCACCCT GTCATCAAGA CACCTTGACA TTTGCTCTAA GACCAAGGAA 
GTTAACAACA TGACAATACC ATCAAAGTTA TGGGGATTCT TCTGCAACTC AAGTCAGTTT 
TTTAATGCCA CCTGTGATGA GTACTTTGTT CACAATAATG TCACCTCAAT CCAGGGCATT 
CCAGGGTTGG CTAGTGGCAT CATTACTGAA AACCTTTGGA GTAATTATTT ACCAAAAGGT 
GAGATAATTG AAAAGCCATC AGCCAAGTCA TCTGACGTTT TGGGCAACTT AAACCATGAA 
TATGTTCTTG CTGATATTAC CACCTCCTTC ACTCTTCTGG TGGGGATCTT CTTTCCTTCT 
GTCACAGGTA TCATGGCTGG GTCAAACAGA TCTGGAGATC TGAAAGATGC TCAAAAGTCT 
ATCCCCATTG GGACCATCCT TGCCATCCTG ACCACATCCT TCGTGTATTT AAGCAATGTT 
GTCCTTTTTG GTGCATGTAT CGAAGGAGTT GTCCTCAGAG ACAAATTTGG GGATGCTGTA 
AAAGGGAATT TGGTTGTAGG CACCTTATCC TGGCCATCCC CATGGGTGAT TGTTATTGGC 
TCCTTCTTTT CAACGTGTGG AGCTGGACTT CAGAGCCTCA CAGGTGCACC TCGGCTGCTG 
CAGGCTATTG CCAAGGACAA CATCATACCT TTCCTTAGGG TTTTTGGACA CAGTAAAGCT 
AATGGGGAAC CTACCTGGGC CTTACTTCTA ACTGCTGCCA TAGCAGAGCT GGGAATTCTC 
ATCGCCTCCC TGGATCTCGT GGCCCCAATT CTTTCCATGT TTTTTCTCAT GTGTTACCTC 
TTTGTGAACT TGGCTTGTGC CTTGCAAACA TTACTGCGAA CCCCCCACTG GAGGCCTCGA 
TTCCGCTACT ATCACTGGGC CCTCTCTTTC ATGGGAATGA GTATCTGTCT AGCTCTGATG 
TTCATTTCTT CTTGGTATTA TGCCATTGTA GCTATGGTAA TAGCTGGCAT GATCTACAAG 
TACATTGAAT ACCAAGGGGC TGAAAAAGAA TGGGGCGATG GTATCCGTGG GCTGTCACTC 
AGTGCAGCCC GCTTCGCTTT GCTCCGCCTA GAGGAAGGCC CTCCTCACAC TAAAAACTGG 
AGGCCTCAGC TGCTTGTACT GCTAAAGCTG GATGAAGACT TACACGTCAA GCACCCTCGC 
CTCCTCACCT TTGCCTCACA GCTCAAGGCA GGAAAGGGAC TCACCATTGT GGGCTCTGTC 
ATCGTGGGGA ACTTCCTGGA GAACTATGGT GACGCTCTAG CAGCAGAGCA GACCATTAAG 
CACCTCATGG AGGCAGAAAA GGTAAAAGGG TTCTGCCAGC TGGTAGTGGC TGCCAAGCTG 
AAAGAGGGCA TTTCACACCT CATCCAGTCC TGTGGCCTTG GAGGCATGAA GCACAACACA 
GTGGTGATGG GCTGGCCCAA TGGCTGGCGT CAGAGTGAAG ATGCTCGTGC TTGGAAGACT 
TTCATTGGCA CAGTTCGAGT GACAACTGCT GCCCATCTAG CCCTGTTAGT GGCTAAAAAT 
GTCTCCTTCT TTCCCAGCAA TGTGGAACAA TTTTCTGAGG GCAACATTGA TGTGTGGTGG 
ATTGTGCATG ATGGGGGCAT GCTCATGCTA TTACCATTCC TACTGAAACA GCACAAGGTT 
TGGCGAAAAT GCAGCATACG GATCTTCACA GTAGCCCAAC TAGAAGACAA CAGTATCCAG 
ATGAAGAAGG ACCTGGCCAC CTTCCTGTAC CATCTGCGCA TCGAGGCCGA AGTGGAAGTG 
GTGGAGATGC ATGACAGTGA CATATCTGCC TATACTTATG AGCGCACCCT GATGATGGAG 
CAGAGGTCCC AGATGCTTCG GCACATGCGG CTGTCCAAAA CAGAACGAGA TAGAGAGGCA 
CAACTGGTGA AAGATCGAAA CTCAATGCTT CGCTTAACTA GCATTGGCTC TGACGAGGAT 
GAAGAAACAG AAACTTACCA AGAGAAGGTG CACATGACTT GGACCAAGGA TAAATACATG 
GCATCCCGGG GGCAAAAGGT CAAATCAATG GAAGGATTCC AAGACCTACT TAATATGCGT 
CCGGACCAGT CCAACGTGAG ACGGATGCAT ACAGCAGTGA AGCTCAATGA AGTTATAGTT 
AACAAGTCCC ACGAAGCAAA GCTGGTTTTG TTGAATATGC CAGGACCACC CCGAAACCCT 
GAAGGTGATG AGAACTACAT GGAATTTCTA GAAGTGCTCA CTGAGGGATT AGAGCGAGTC 
CTCCTCGTCC GGGGTGGTGG CAGTGAAGTC ATCACCATAT ACTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006234772.1
CDS	1029..4436
Translation	MHPPETTTKMSSVRFMVTPTKIDDIPGLSDTSPDLSSRSSSRVRFSSRESVPETSRSEPMSELSGATTSLATVALDPSSDRTSNPQDVTEDDGHKKARNAYLNNSNYEEGDEYFDKNLALFEEEMDTRPKVSSLLNRMANYTNLTQGAKEHEEAENITEGKKKPTKSPQMGTFMGVYLPCLQNIFGVILFLRLTWVVGTAGVLQAFAIVLICCCCTMLTAISMSAIATNGVVPAGGSYFMISRALGPEFGGAVGLCFYLGTTFAAAMYILGAIEIFLVYIVPRAAIFRSDDALKESAAMLNNMRVYGTAFLVLMVLVVFIGVRYVNKFASLFLACVIVSILAIYAGAIKSSFAPPHFPVCMLGNRTLSSRHLDICSKTKEVNNMTIPSKLWGFFCNSSQFFNATCDEYFVHNNVTSIQGIPGLASGIITENLWSNYLPKGEIIEKPSAKSSDVLGNLNHEYVLADITTSFTLLVGIFFPSVTGIMAGSNRSGDLKDAQKSIPIGTILAILTTSFVYLSNVVLFGACIEGVVLRDKFGDAVKGNLVVGTLSWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAIAKDNIIPFLRVFGHSKANGEPTWALLLTAAIAELGILIASLDLVAPILSMFFLMCYLFVNLACALQTLLRTPHWRPRFRYYHWALSFMGMSICLALMFISSWYYAIVAMVIAGMIYKYIEYQGAEKEWGDGIRGLSLSAARFALLRLEEGPPHTKNWRPQLLVLLKLDEDLHVKHPRLLTFASQLKAGKGLTIVGSVIVGNFLENYGDALAAEQTIKHLMEAEKVKGFCQLVVAAKLKEGISHLIQSCGLGGMKHNTVVMGWPNGWRQSEDARAWKTFIGTVRVTTAAHLALLVAKNVSFFPSNVEQFSEGNIDVWWIVHDGGMLMLLPFLLKQHKVWRKCSIRIFTVAQLEDNSIQMKKDLATFLYHLRIEAEVEVVEMHDSDISAYTYERTLMMEQRSQMLRHMRLSKTERDREAQLVKDRNSMLRLTSIGSDEDEETETYQEKVHMTWTKDKYMASRGQKVKSMEGFQDLLNMRPDQSNVRRMHTAVKLNEVIVNKSHEAKLVLLNMPGPPRNPEGDENYMEFLEVLTEGLERVLLVRGGGSEVITIYS

Target ORF information:

RefSeq Version	XM_006234710.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus solute carrier family 12, member 6 (Slc12a6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006234710.3

ORF Insert Sequence:

ATGCATCCTC CAGAAACCAC CACCAAGATG TCTTCAGTTC GGTTCATGGT GACACCAACA 
AAGATTGATG ACATTCCAGG TTTATCAGAC ACCAGTCCGG ACCTCAGCTC TCGATCTAGT 
TCTCGAGTAA GATTTAGCTC CCGAGAAAGT GTGCCAGAAA CAAGCCGTAG TGAGCCTATG 
AGTGAACTGT CTGGGGCCAC CACTTCTCTG GCAACTGTTG CCCTAGATCC TTCCAGTGAC 
CGGACTTCTA ACCCCCAGGA TGTTACCGAG GATGATGGAC ATAAGAAAGC TCGAAATGCT 
TATCTCAATA ATTCCAACTA TGAAGAAGGA GACGAATATT TTGATAAAAA TCTGGCACTC 
TTTGAGGAAG AAATGGACAC CAGACCAAAG GTGTCTTCTC TCCTCAACCG CATGGCCAAC 
TATACAAATC TGACACAAGG AGCAAAGGAA CATGAAGAGG CAGAGAACAT CACCGAAGGG 
AAAAAGAAGC CTACCAAGAG CCCCCAAATG GGTACTTTCA TGGGTGTCTA CCTCCCATGT 
CTACAAAATA TTTTTGGAGT GATCCTCTTT CTGCGTCTTA CCTGGGTAGT GGGCACAGCT 
GGAGTCCTTC AGGCCTTTGC CATTGTCCTT ATCTGCTGCT GCTGTACAAT GTTAACTGCC 
ATCTCCATGA GTGCCATCGC CACTAACGGA GTGGTGCCAG CTGGAGGCTC ATACTTCATG 
ATTTCCCGGG CCCTGGGCCC AGAGTTTGGC GGGGCTGTGG GCCTCTGCTT TTATCTTGGC 
ACCACATTTG CAGCAGCCAT GTATATTCTT GGTGCCATTG AAATCTTTCT GGTGTATATT 
GTCCCCCGAG CTGCCATCTT TCGGAGCGAT GATGCACTCA AGGAGTCAGC AGCCATGCTG 
AACAACATGC GTGTCTATGG TACAGCCTTC CTGGTCCTCA TGGTCTTGGT GGTGTTCATC 
GGCGTACGCT ATGTGAATAA GTTTGCCTCA CTTTTCCTGG CCTGTGTAAT TGTGTCGATC 
TTGGCTATCT ATGCTGGTGC CATCAAGTCT TCCTTTGCTC CACCACACTT CCCGGTCTGT 
ATGCTGGGCA ACCGCACCCT GTCATCAAGA CACCTTGACA TTTGCTCTAA GACCAAGGAA 
GTTAACAACA TGACAATACC ATCAAAGTTA TGGGGATTCT TCTGCAACTC AAGTCAGTTT 
TTTAATGCCA CCTGTGATGA GTACTTTGTT CACAATAATG TCACCTCAAT CCAGGGCATT 
CCAGGGTTGG CTAGTGGCAT CATTACTGAA AACCTTTGGA GTAATTATTT ACCAAAAGGT 
GAGATAATTG AAAAGCCATC AGCCAAGTCA TCTGACGTTT TGGGCAACTT AAACCATGAA 
TATGTTCTTG CTGATATTAC CACCTCCTTC ACTCTTCTGG TGGGGATCTT CTTTCCTTCT 
GTCACAGGTA TCATGGCTGG GTCAAACAGA TCTGGAGATC TGAAAGATGC TCAAAAGTCT 
ATCCCCATTG GGACCATCCT TGCCATCCTG ACCACATCCT TCGTGTATTT AAGCAATGTT 
GTCCTTTTTG GTGCATGTAT CGAAGGAGTT GTCCTCAGAG ACAAATTTGG GGATGCTGTA 
AAAGGGAATT TGGTTGTAGG CACCTTATCC TGGCCATCCC CATGGGTGAT TGTTATTGGC 
TCCTTCTTTT CAACGTGTGG AGCTGGACTT CAGAGCCTCA CAGGTGCACC TCGGCTGCTG 
CAGGCTATTG CCAAGGACAA CATCATACCT TTCCTTAGGG TTTTTGGACA CAGTAAAGCT 
AATGGGGAAC CTACCTGGGC CTTACTTCTA ACTGCTGCCA TAGCAGAGCT GGGAATTCTC 
ATCGCCTCCC TGGATCTCGT GGCCCCAATT CTTTCCATGT TTTTTCTCAT GTGTTACCTC 
TTTGTGAACT TGGCTTGTGC CTTGCAAACA TTACTGCGAA CCCCCCACTG GAGGCCTCGA 
TTCCGCTACT ATCACTGGGC CCTCTCTTTC ATGGGAATGA GTATCTGTCT AGCTCTGATG 
TTCATTTCTT CTTGGTATTA TGCCATTGTA GCTATGGTAA TAGCTGGCAT GATCTACAAG 
TACATTGAAT ACCAAGGGGC TGAAAAAGAA TGGGGCGATG GTATCCGTGG GCTGTCACTC 
AGTGCAGCCC GCTTCGCTTT GCTCCGCCTA GAGGAAGGCC CTCCTCACAC TAAAAACTGG 
AGGCCTCAGC TGCTTGTACT GCTAAAGCTG GATGAAGACT TACACGTCAA GCACCCTCGC 
CTCCTCACCT TTGCCTCACA GCTCAAGGCA GGAAAGGGAC TCACCATTGT GGGCTCTGTC 
ATCGTGGGGA ACTTCCTGGA GAACTATGGT GACGCTCTAG CAGCAGAGCA GACCATTAAG 
CACCTCATGG AGGCAGAAAA GGTAAAAGGG TTCTGCCAGC TGGTAGTGGC TGCCAAGCTG 
AAAGAGGGCA TTTCACACCT CATCCAGTCC TGTGGCCTTG GAGGCATGAA GCACAACACA 
GTGGTGATGG GCTGGCCCAA TGGCTGGCGT CAGAGTGAAG ATGCTCGTGC TTGGAAGACT 
TTCATTGGCA CAGTTCGAGT GACAACTGCT GCCCATCTAG CCCTGTTAGT GGCTAAAAAT 
GTCTCCTTCT TTCCCAGCAA TGTGGAACAA TTTTCTGAGG GCAACATTGA TGTGTGGTGG 
ATTGTGCATG ATGGGGGCAT GCTCATGCTA TTACCATTCC TACTGAAACA GCACAAGGTT 
TGGCGAAAAT GCAGCATACG GATCTTCACA GTAGCCCAAC TAGAAGACAA CAGTATCCAG 
ATGAAGAAGG ACCTGGCCAC CTTCCTGTAC CATCTGCGCA TCGAGGCCGA AGTGGAAGTG 
GTGGAGATGC ATGACAGTGA CATATCTGCC TATACTTATG AGCGCACCCT GATGATGGAG 
CAGAGGTCCC AGATGCTTCG GCACATGCGG CTGTCCAAAA CAGAACGAGA TAGAGAGGCA 
CAACTGGTGA AAGATCGAAA CTCAATGCTT CGCTTAACTA GCATTGGCTC TGACGAGGAT 
GAAGAAACAG AAACTTACCA AGAGAAGGTG CACATGACTT GGACCAAGGA TAAATACATG 
GCATCCCGGG GGCAAAAGGT CAAATCAATG GAAGGATTCC AAGACCTACT TAATATGCGT 
CCGGACCAGT CCAACGTGAG ACGGATGCAT ACAGCAGTGA AGCTCAATGA AGTTATAGTT 
AACAAGTCCC ACGAAGCAAA GCTGGTTTTG TTGAATATGC CAGGACCACC CCGAAACCCT 
GAAGGTGATG AGAACTACAT GGAATTTCTA GAAGTGCTCA CTGAGGGATT AGAGCGAGTC 
CTCCTCGTCC GGGGTGGTGG CAGTGAAGTC ATCACCATAT ACTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slc12a6 ( XM_006234711.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006234773.1 Rattus norvegicus solute carrier family 12, member 6 (Slc12a6), transcript variant X2, mRNA.

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CloneID	ORa26190
Clone ID Related Accession (Same CDS sequence)	XM_006234711.3
Accession Version	XM_006234711.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3300bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	solute carrier family 12 member 6 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006234711.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGCACT TCACCGTGAC CAAGGTAGAA GACCCAGAGG AGGGGGCAGC TGGCTCCCTT 
TCTCCTGAGC CCAGCTCAGC AGCAGTAAAA GCCCGGATTC AGGATCCCGA AGAACCAGAC 
CCGAATCAGA ACTCCATCAC AGGGGAGCAC AGCCAGCTGT TAGATGATGG ACATAAGAAA 
GCTCGAAATG CTTATCTCAA TAATTCCAAC TATGAAGAAG GAGACGAATA TTTTGATAAA 
AATCTGGCAC TCTTTGAGGA AGAAATGGAC ACCAGACCAA AGGTGTCTTC TCTCCTCAAC 
CGCATGGCCA ACTATACAAA TCTGACACAA GGAGCAAAGG AACATGAAGA GGCAGAGAAC 
ATCACCGAAG GGAAAAAGAA GCCTACCAAG AGCCCCCAAA TGGGTACTTT CATGGGTGTC 
TACCTCCCAT GTCTACAAAA TATTTTTGGA GTGATCCTCT TTCTGCGTCT TACCTGGGTA 
GTGGGCACAG CTGGAGTCCT TCAGGCCTTT GCCATTGTCC TTATCTGCTG CTGCTGTACA 
ATGTTAACTG CCATCTCCAT GAGTGCCATC GCCACTAACG GAGTGGTGCC AGCTGGAGGC 
TCATACTTCA TGATTTCCCG GGCCCTGGGC CCAGAGTTTG GCGGGGCTGT GGGCCTCTGC 
TTTTATCTTG GCACCACATT TGCAGCAGCC ATGTATATTC TTGGTGCCAT TGAAATCTTT 
CTGGTGTATA TTGTCCCCCG AGCTGCCATC TTTCGGAGCG ATGATGCACT CAAGGAGTCA 
GCAGCCATGC TGAACAACAT GCGTGTCTAT GGTACAGCCT TCCTGGTCCT CATGGTCTTG 
GTGGTGTTCA TCGGCGTACG CTATGTGAAT AAGTTTGCCT CACTTTTCCT GGCCTGTGTA 
ATTGTGTCGA TCTTGGCTAT CTATGCTGGT GCCATCAAGT CTTCCTTTGC TCCACCACAC 
TTCCCGGTCT GTATGCTGGG CAACCGCACC CTGTCATCAA GACACCTTGA CATTTGCTCT 
AAGACCAAGG AAGTTAACAA CATGACAATA CCATCAAAGT TATGGGGATT CTTCTGCAAC 
TCAAGTCAGT TTTTTAATGC CACCTGTGAT GAGTACTTTG TTCACAATAA TGTCACCTCA 
ATCCAGGGCA TTCCAGGGTT GGCTAGTGGC ATCATTACTG AAAACCTTTG GAGTAATTAT 
TTACCAAAAG GTGAGATAAT TGAAAAGCCA TCAGCCAAGT CATCTGACGT TTTGGGCAAC 
TTAAACCATG AATATGTTCT TGCTGATATT ACCACCTCCT TCACTCTTCT GGTGGGGATC 
TTCTTTCCTT CTGTCACAGG TATCATGGCT GGGTCAAACA GATCTGGAGA TCTGAAAGAT 
GCTCAAAAGT CTATCCCCAT TGGGACCATC CTTGCCATCC TGACCACATC CTTCGTGTAT 
TTAAGCAATG TTGTCCTTTT TGGTGCATGT ATCGAAGGAG TTGTCCTCAG AGACAAATTT 
GGGGATGCTG TAAAAGGGAA TTTGGTTGTA GGCACCTTAT CCTGGCCATC CCCATGGGTG 
ATTGTTATTG GCTCCTTCTT TTCAACGTGT GGAGCTGGAC TTCAGAGCCT CACAGGTGCA 
CCTCGGCTGC TGCAGGCTAT TGCCAAGGAC AACATCATAC CTTTCCTTAG GGTTTTTGGA 
CACAGTAAAG CTAATGGGGA ACCTACCTGG GCCTTACTTC TAACTGCTGC CATAGCAGAG 
CTGGGAATTC TCATCGCCTC CCTGGATCTC GTGGCCCCAA TTCTTTCCAT GTTTTTTCTC 
ATGTGTTACC TCTTTGTGAA CTTGGCTTGT GCCTTGCAAA CATTACTGCG AACCCCCCAC 
TGGAGGCCTC GATTCCGCTA CTATCACTGG GCCCTCTCTT TCATGGGAAT GAGTATCTGT 
CTAGCTCTGA TGTTCATTTC TTCTTGGTAT TATGCCATTG TAGCTATGGT AATAGCTGGC 
ATGATCTACA AGTACATTGA ATACCAAGGG GCTGAAAAAG AATGGGGCGA TGGTATCCGT 
GGGCTGTCAC TCAGTGCAGC CCGCTTCGCT TTGCTCCGCC TAGAGGAAGG CCCTCCTCAC 
ACTAAAAACT GGAGGCCTCA GCTGCTTGTA CTGCTAAAGC TGGATGAAGA CTTACACGTC 
AAGCACCCTC GCCTCCTCAC CTTTGCCTCA CAGCTCAAGG CAGGAAAGGG ACTCACCATT 
GTGGGCTCTG TCATCGTGGG GAACTTCCTG GAGAACTATG GTGACGCTCT AGCAGCAGAG 
CAGACCATTA AGCACCTCAT GGAGGCAGAA AAGGTAAAAG GGTTCTGCCA GCTGGTAGTG 
GCTGCCAAGC TGAAAGAGGG CATTTCACAC CTCATCCAGT CCTGTGGCCT TGGAGGCATG 
AAGCACAACA CAGTGGTGAT GGGCTGGCCC AATGGCTGGC GTCAGAGTGA AGATGCTCGT 
GCTTGGAAGA CTTTCATTGG CACAGTTCGA GTGACAACTG CTGCCCATCT AGCCCTGTTA 
GTGGCTAAAA ATGTCTCCTT CTTTCCCAGC AATGTGGAAC AATTTTCTGA GGGCAACATT 
GATGTGTGGT GGATTGTGCA TGATGGGGGC ATGCTCATGC TATTACCATT CCTACTGAAA 
CAGCACAAGG TTTGGCGAAA ATGCAGCATA CGGATCTTCA CAGTAGCCCA ACTAGAAGAC 
AACAGTATCC AGATGAAGAA GGACCTGGCC ACCTTCCTGT ACCATCTGCG CATCGAGGCC 
GAAGTGGAAG TGGTGGAGAT GCATGACAGT GACATATCTG CCTATACTTA TGAGCGCACC 
CTGATGATGG AGCAGAGGTC CCAGATGCTT CGGCACATGC GGCTGTCCAA AACAGAACGA 
GATAGAGAGG CACAACTGGT GAAAGATCGA AACTCAATGC TTCGCTTAAC TAGCATTGGC 
TCTGACGAGG ATGAAGAAAC AGAAACTTAC CAAGAGAAGG TGCACATGAC TTGGACCAAG 
GATAAATACA TGGCATCCCG GGGGCAAAAG GTCAAATCAA TGGAAGGATT CCAAGACCTA 
CTTAATATGC GTCCGGACCA GTCCAACGTG AGACGGATGC ATACAGCAGT GAAGCTCAAT 
GAAGTTATAG TTAACAAGTC CCACGAAGCA AAGCTGGTTT TGTTGAATAT GCCAGGACCA 
CCCCGAAACC CTGAAGGTGA TGAGAACTAC ATGGAATTTC TAGAAGTGCT CACTGAGGGA 
TTAGAGCGAG TCCTCCTCGT CCGGGGTGGT GGCAGTGAAG TCATCACCAT ATACTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006234773.1
CDS	3973..7272
Translation	MPHFTVTKVEDPEEGAAGSLSPEPSSAAVKARIQDPEEPDPNQNSITGEHSQLLDDGHKKARNAYLNNSNYEEGDEYFDKNLALFEEEMDTRPKVSSLLNRMANYTNLTQGAKEHEEAENITEGKKKPTKSPQMGTFMGVYLPCLQNIFGVILFLRLTWVVGTAGVLQAFAIVLICCCCTMLTAISMSAIATNGVVPAGGSYFMISRALGPEFGGAVGLCFYLGTTFAAAMYILGAIEIFLVYIVPRAAIFRSDDALKESAAMLNNMRVYGTAFLVLMVLVVFIGVRYVNKFASLFLACVIVSILAIYAGAIKSSFAPPHFPVCMLGNRTLSSRHLDICSKTKEVNNMTIPSKLWGFFCNSSQFFNATCDEYFVHNNVTSIQGIPGLASGIITENLWSNYLPKGEIIEKPSAKSSDVLGNLNHEYVLADITTSFTLLVGIFFPSVTGIMAGSNRSGDLKDAQKSIPIGTILAILTTSFVYLSNVVLFGACIEGVVLRDKFGDAVKGNLVVGTLSWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAIAKDNIIPFLRVFGHSKANGEPTWALLLTAAIAELGILIASLDLVAPILSMFFLMCYLFVNLACALQTLLRTPHWRPRFRYYHWALSFMGMSICLALMFISSWYYAIVAMVIAGMIYKYIEYQGAEKEWGDGIRGLSLSAARFALLRLEEGPPHTKNWRPQLLVLLKLDEDLHVKHPRLLTFASQLKAGKGLTIVGSVIVGNFLENYGDALAAEQTIKHLMEAEKVKGFCQLVVAAKLKEGISHLIQSCGLGGMKHNTVVMGWPNGWRQSEDARAWKTFIGTVRVTTAAHLALLVAKNVSFFPSNVEQFSEGNIDVWWIVHDGGMLMLLPFLLKQHKVWRKCSIRIFTVAQLEDNSIQMKKDLATFLYHLRIEAEVEVVEMHDSDISAYTYERTLMMEQRSQMLRHMRLSKTERDREAQLVKDRNSMLRLTSIGSDEDEETETYQEKVHMTWTKDKYMASRGQKVKSMEGFQDLLNMRPDQSNVRRMHTAVKLNEVIVNKSHEAKLVLLNMPGPPRNPEGDENYMEFLEVLTEGLERVLLVRGGGSEVITIYS

Target ORF information:

RefSeq Version	XM_006234711.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus solute carrier family 12, member 6 (Slc12a6), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006234711.3

ORF Insert Sequence:

ATGCCGCACT TCACCGTGAC CAAGGTAGAA GACCCAGAGG AGGGGGCAGC TGGCTCCCTT 
TCTCCTGAGC CCAGCTCAGC AGCAGTAAAA GCCCGGATTC AGGATCCCGA AGAACCAGAC 
CCGAATCAGA ACTCCATCAC AGGGGAGCAC AGCCAGCTGT TAGATGATGG ACATAAGAAA 
GCTCGAAATG CTTATCTCAA TAATTCCAAC TATGAAGAAG GAGACGAATA TTTTGATAAA 
AATCTGGCAC TCTTTGAGGA AGAAATGGAC ACCAGACCAA AGGTGTCTTC TCTCCTCAAC 
CGCATGGCCA ACTATACAAA TCTGACACAA GGAGCAAAGG AACATGAAGA GGCAGAGAAC 
ATCACCGAAG GGAAAAAGAA GCCTACCAAG AGCCCCCAAA TGGGTACTTT CATGGGTGTC 
TACCTCCCAT GTCTACAAAA TATTTTTGGA GTGATCCTCT TTCTGCGTCT TACCTGGGTA 
GTGGGCACAG CTGGAGTCCT TCAGGCCTTT GCCATTGTCC TTATCTGCTG CTGCTGTACA 
ATGTTAACTG CCATCTCCAT GAGTGCCATC GCCACTAACG GAGTGGTGCC AGCTGGAGGC 
TCATACTTCA TGATTTCCCG GGCCCTGGGC CCAGAGTTTG GCGGGGCTGT GGGCCTCTGC 
TTTTATCTTG GCACCACATT TGCAGCAGCC ATGTATATTC TTGGTGCCAT TGAAATCTTT 
CTGGTGTATA TTGTCCCCCG AGCTGCCATC TTTCGGAGCG ATGATGCACT CAAGGAGTCA 
GCAGCCATGC TGAACAACAT GCGTGTCTAT GGTACAGCCT TCCTGGTCCT CATGGTCTTG 
GTGGTGTTCA TCGGCGTACG CTATGTGAAT AAGTTTGCCT CACTTTTCCT GGCCTGTGTA 
ATTGTGTCGA TCTTGGCTAT CTATGCTGGT GCCATCAAGT CTTCCTTTGC TCCACCACAC 
TTCCCGGTCT GTATGCTGGG CAACCGCACC CTGTCATCAA GACACCTTGA CATTTGCTCT 
AAGACCAAGG AAGTTAACAA CATGACAATA CCATCAAAGT TATGGGGATT CTTCTGCAAC 
TCAAGTCAGT TTTTTAATGC CACCTGTGAT GAGTACTTTG TTCACAATAA TGTCACCTCA 
ATCCAGGGCA TTCCAGGGTT GGCTAGTGGC ATCATTACTG AAAACCTTTG GAGTAATTAT 
TTACCAAAAG GTGAGATAAT TGAAAAGCCA TCAGCCAAGT CATCTGACGT TTTGGGCAAC 
TTAAACCATG AATATGTTCT TGCTGATATT ACCACCTCCT TCACTCTTCT GGTGGGGATC 
TTCTTTCCTT CTGTCACAGG TATCATGGCT GGGTCAAACA GATCTGGAGA TCTGAAAGAT 
GCTCAAAAGT CTATCCCCAT TGGGACCATC CTTGCCATCC TGACCACATC CTTCGTGTAT 
TTAAGCAATG TTGTCCTTTT TGGTGCATGT ATCGAAGGAG TTGTCCTCAG AGACAAATTT 
GGGGATGCTG TAAAAGGGAA TTTGGTTGTA GGCACCTTAT CCTGGCCATC CCCATGGGTG 
ATTGTTATTG GCTCCTTCTT TTCAACGTGT GGAGCTGGAC TTCAGAGCCT CACAGGTGCA 
CCTCGGCTGC TGCAGGCTAT TGCCAAGGAC AACATCATAC CTTTCCTTAG GGTTTTTGGA 
CACAGTAAAG CTAATGGGGA ACCTACCTGG GCCTTACTTC TAACTGCTGC CATAGCAGAG 
CTGGGAATTC TCATCGCCTC CCTGGATCTC GTGGCCCCAA TTCTTTCCAT GTTTTTTCTC 
ATGTGTTACC TCTTTGTGAA CTTGGCTTGT GCCTTGCAAA CATTACTGCG AACCCCCCAC 
TGGAGGCCTC GATTCCGCTA CTATCACTGG GCCCTCTCTT TCATGGGAAT GAGTATCTGT 
CTAGCTCTGA TGTTCATTTC TTCTTGGTAT TATGCCATTG TAGCTATGGT AATAGCTGGC 
ATGATCTACA AGTACATTGA ATACCAAGGG GCTGAAAAAG AATGGGGCGA TGGTATCCGT 
GGGCTGTCAC TCAGTGCAGC CCGCTTCGCT TTGCTCCGCC TAGAGGAAGG CCCTCCTCAC 
ACTAAAAACT GGAGGCCTCA GCTGCTTGTA CTGCTAAAGC TGGATGAAGA CTTACACGTC 
AAGCACCCTC GCCTCCTCAC CTTTGCCTCA CAGCTCAAGG CAGGAAAGGG ACTCACCATT 
GTGGGCTCTG TCATCGTGGG GAACTTCCTG GAGAACTATG GTGACGCTCT AGCAGCAGAG 
CAGACCATTA AGCACCTCAT GGAGGCAGAA AAGGTAAAAG GGTTCTGCCA GCTGGTAGTG 
GCTGCCAAGC TGAAAGAGGG CATTTCACAC CTCATCCAGT CCTGTGGCCT TGGAGGCATG 
AAGCACAACA CAGTGGTGAT GGGCTGGCCC AATGGCTGGC GTCAGAGTGA AGATGCTCGT 
GCTTGGAAGA CTTTCATTGG CACAGTTCGA GTGACAACTG CTGCCCATCT AGCCCTGTTA 
GTGGCTAAAA ATGTCTCCTT CTTTCCCAGC AATGTGGAAC AATTTTCTGA GGGCAACATT 
GATGTGTGGT GGATTGTGCA TGATGGGGGC ATGCTCATGC TATTACCATT CCTACTGAAA 
CAGCACAAGG TTTGGCGAAA ATGCAGCATA CGGATCTTCA CAGTAGCCCA ACTAGAAGAC 
AACAGTATCC AGATGAAGAA GGACCTGGCC ACCTTCCTGT ACCATCTGCG CATCGAGGCC 
GAAGTGGAAG TGGTGGAGAT GCATGACAGT GACATATCTG CCTATACTTA TGAGCGCACC 
CTGATGATGG AGCAGAGGTC CCAGATGCTT CGGCACATGC GGCTGTCCAA AACAGAACGA 
GATAGAGAGG CACAACTGGT GAAAGATCGA AACTCAATGC TTCGCTTAAC TAGCATTGGC 
TCTGACGAGG ATGAAGAAAC AGAAACTTAC CAAGAGAAGG TGCACATGAC TTGGACCAAG 
GATAAATACA TGGCATCCCG GGGGCAAAAG GTCAAATCAA TGGAAGGATT CCAAGACCTA 
CTTAATATGC GTCCGGACCA GTCCAACGTG AGACGGATGC ATACAGCAGT GAAGCTCAAT 
GAAGTTATAG TTAACAAGTC CCACGAAGCA AAGCTGGTTT TGTTGAATAT GCCAGGACCA 
CCCCGAAACC CTGAAGGTGA TGAGAACTAC ATGGAATTTC TAGAAGTGCT CACTGAGGGA 
TTAGAGCGAG TCCTCCTCGT CCGGGGTGGT GGCAGTGAAG TCATCACCAT ATACTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The WNK-regulated SPAK/OSR1 kinases directly phosphorylate and inhibit the K+-Cl- co-transporters.
The Biochemical journal458(3)559-73(2014 Mar)
de Los Heros P,Alessi DR,Gourlay R,Campbell DG,Deak M,Macartney TJ,Kahle KT,Zhang J

Molecular evidence for a role for K(+)-Cl(-) cotransporters in the kidney.
American journal of physiology. Renal physiology305(10)F1402-11(2013 Nov)
Melo Z,Cruz-Rangel S,Bautista R,Vázquez N,Castañeda-Bueno M,Mount DB,Pasantes-Morales H,Mercado A,Gamba G

Role of cholesterol in functional association between K(+)-Cl(-) cotransporter-3a and Na+,K(+)-ATPase.
Biochemical and biophysical research communications424(1)136-40(2012 Jul)
Fujita K,Fujii T,Shimizu T,Takeguchi N,Sakai H

High-grade glioma motility reduced by genetic knockdown of KCC3.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology30(2)466-76(2012)
Gagnon KB

The NH(2)-terminus of K(+)-Cl(-) cotransporter 3a is essential for up-regulation of Na(+),K(+)-ATPase activity.
Biochemical and biophysical research communications399(4)683-7(2010 Sep)
Fujii T,Fujita K,Shimizu T,Takeguchi N,Sakai H

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