Ckmt2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ckmt2
Entrez Gene ID	688698
Full Name	creatine kinase, mitochondrial 2
General protein information	Preferred Names creatine kinase, mitochondrial 2 Names creatine kinase S-type, mitochondrial S-MtCK basic-type mitochondrial creatine kinase creatine kinase, mitochondrial 2, sarcomeric mib-CK sarcomeric mitochondrial creatine kinase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q12
Summary	sarcomeric mitochondrial creatine kinase isozyme; catalyzes the transfer of phosphate to creatine in energy transduction [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001127652.1	NP_001121124.1	creatine kinase S-type, mitochondrial precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	CKMT2	NP_001816.2
Pan troglodytes (chimpanzee)	CKMT2	XP_001149997.1
Mus musculus (house mouse)	Ckmt2	NP_940807.1
Macaca mulatta (Rhesus monkey)	CKMT2	XP_001110817.1
Canis lupus familiaris (dog)	CKMT2	NP_001138686.1
Rattus norvegicus (Norway rat)	Ckmt2	NP_001121124.1
Bos taurus (cattle)	CKMT2	NP_001029828.1
Gallus gallus (chicken)	CKMT2	NP_001161216.1
Danio rerio (zebrafish)	ckmt2b	NP_956991.1

Gene Ontology
Bibliography

Related articles in PubMed

Phosphoproteome analysis of functional mitochondria isolated from resting human muscle reveals extensive phosphorylation of inner membrane protein complexes and enzymes.
Zhao X, León IR, Bak S, Mogensen M, Wrzesinski K, Højlund K, Jensen ON
Molecular & cellular proteomics : MCP10(1)M110.000299(2011 Jan)

Developmental expression of sarcomeric and ubiquitous mitochondrial creatine kinase is tissue-specific.
Payne RM, Strauss AW
Biochimica et biophysica acta1219(1)33-8(1994 Sep)

Structural characterization and tissue-specific expression of the mRNAs encoding isoenzymes from two rat mitochondrial creatine kinase genes.
Payne RM, Haas RC, Strauss AW
Biochimica et biophysica acta1089(3)352-61(1991 Jul)

Identification and primary structure of the cardiolipin-binding domain of mitochondrial creatine kinase.
Cheneval D, Carafoli E
European journal of biochemistry171(1-2)1-9(1988 Jan)

Cardiolipin is the membrane receptor for mitochondrial creatine phosphokinase.
Müller M, Moser R, Cheneval D, Carafoli E
The Journal of biological chemistry260(6)3839-43(1985 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ckmt2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ckmt2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa42647	NM_001127652.1 Latest version!	Rattus norvegicus creatine kinase, mitochondrial 2 (Ckmt2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ckmt2 ( NM_001127652.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001121124.1 Rattus norvegicus creatine kinase, mitochondrial 2 (Ckmt2), mRNA.

Price & Availability↑

CloneID	ORa42647
Clone ID Related Accession (Same CDS sequence)	NM_001127652.1
Accession Version	NM_001127652.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1260bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1568908800000
Organism	Rattus norvegicus(Norway rat)
Product	creatine kinase S-type, mitochondrial precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC158762.1. ##Evidence-Data-START## Transcript exon combination :: BC158762.1, FQ216119.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2689596, SAMEA2689599 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGCCAGTG CCTTCTCTAA GTTGCTAACT GGCCGAAATG CATCTCTGCT GTTTACGACA 
CTGGGAACCA GTGCCCTGAC CACGGGCTAC CTGTTGAATA GGCAGAAGGT ATCTGCTGAT 
GCCCGGGAAC AGCACAAGCT CTTTCCCCCA AGTGCAGACT ATCCTGACCT TCGAAAACAT 
AACAACTGCA TGGCCGAGTG CCTCACCCCA ACCATCTATG CCAAACTCCG AAACAAGATG 
ACACCCAGTG GCTATACCCT GGACCAGTGC ATCCAAACCG GAGTGGACAA CCCCGGCCAC 
CCCTTCATTA AGACTGTGGG CATGGTGGCT GGTGACGAGG AGTCGTATGA GGTGTTTGCT 
GACCTTTTTG ATCCTGTCAT CAAGTTAAGG CACAATGGCT ATGACCCTAG GGTGATGAAG 
CATCCTACTG ACCTGGATGC ATCCAAGATC ACTCACGGGC AGTTTGATGA ACGCTATGTG 
TTGTCCTCTC GGGTACGCAC TGGCCGCAGC ATCCGCGGGC TGAGCCTGCC TCCTGCCTGC 
TCTCGGGCAG AAAGAAGGGA GGTGGAGAAT GTGGCCATTA CTGCCCTGGA GGGCCTCAAA 
GGGGATCTGG CTGGTCGCTA CTACAAGCTG TCTGAGATGA CAGAGCAGGA TCAACAACGG 
CTCATCGATG ACCACTTTCT GTTTGATAAG CCAGTGTCCC CTTTACTAAC ATGCGCTGGG 
ATGGCCCGTG ACTGGCCTGA TGCCAGAGGA ATCTGGCATA ATTATGACAA GACATTTCTC 
ATCTGGATAA ATGAAGAAGA CCACACCAGG GTGATTTCAA TGGAGAAGGG AGGCAATATG 
AAACGCGTAT TTGAGCGATT CTGTCGTGGA CTAAAGGAAG TGGAACGACT AATCCAAGAA 
CGAGGCTGGG AGTTCATGTG GAATGAACGT CTAGGATACA TTTTGACTTG CCCCTCGAAC 
CTCGGAACTG GATTGCGAGC TGGTGTCCAC GTTAGGATCC CAAAGCTCAG CAAGGATCCA 
CGCTTTTCTA AGATCTTGGA GAACCTGCGG CTCCAGAAGC GTGGCACAGG AGGCGTGGAC 
ACTGCAGCAG TGGCCGACGT GTACGACATT TCCAACATAG ATCGGATCGG TAGATCTGAG 
GTTGAGCTTG TTCAGATTGT TATTGACGGA GTCAACTACC TGGTGGATTG TGAAAAGAAG 
CTGGAGAGAG GCCAGGACAT CAAGGTGCCG CCTCCCCTGC CTCAGTTTGG CAGGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001121124.1
CDS	44..1303
Translation	MASAFSKLLTGRNASLLFTTLGTSALTTGYLLNRQKVSADAREQHKLFPPSADYPDLRKHNNCMAECLTPTIYAKLRNKMTPSGYTLDQCIQTGVDNPGHPFIKTVGMVAGDEESYEVFADLFDPVIKLRHNGYDPRVMKHPTDLDASKITHGQFDERYVLSSRVRTGRSIRGLSLPPACSRAERREVENVAITALEGLKGDLAGRYYKLSEMTEQDQQRLIDDHFLFDKPVSPLLTCAGMARDWPDARGIWHNYDKTFLIWINEEDHTRVISMEKGGNMKRVFERFCRGLKEVERLIQERGWEFMWNERLGYILTCPSNLGTGLRAGVHVRIPKLSKDPRFSKILENLRLQKRGTGGVDTAAVADVYDISNIDRIGRSEVELVQIVIDGVNYLVDCEKKLERGQDIKVPPPLPQFGRK

Target ORF information:

RefSeq Version	NM_001127652.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus creatine kinase, mitochondrial 2 (Ckmt2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001127652.1

ORF Insert Sequence:

ATGGCCAGTG CCTTCTCTAA GTTGCTAACT GGCCGAAATG CATCTCTGCT GTTTACGACA 
CTGGGAACCA GTGCCCTGAC CACGGGCTAC CTGTTGAATA GGCAGAAGGT ATCTGCTGAT 
GCCCGGGAAC AGCACAAGCT CTTTCCCCCA AGTGCAGACT ATCCTGACCT TCGAAAACAT 
AACAACTGCA TGGCCGAGTG CCTCACCCCA ACCATCTATG CCAAACTCCG AAACAAGATG 
ACACCCAGTG GCTATACCCT GGACCAGTGC ATCCAAACCG GAGTGGACAA CCCCGGCCAC 
CCCTTCATTA AGACTGTGGG CATGGTGGCT GGTGACGAGG AGTCGTATGA GGTGTTTGCT 
GACCTTTTTG ATCCTGTCAT CAAGTTAAGG CACAATGGCT ATGACCCTAG GGTGATGAAG 
CATCCTACTG ACCTGGATGC ATCCAAGATC ACTCACGGGC AGTTTGATGA ACGCTATGTG 
TTGTCCTCTC GGGTACGCAC TGGCCGCAGC ATCCGCGGGC TGAGCCTGCC TCCTGCCTGC 
TCTCGGGCAG AAAGAAGGGA GGTGGAGAAT GTGGCCATTA CTGCCCTGGA GGGCCTCAAA 
GGGGATCTGG CTGGTCGCTA CTACAAGCTG TCTGAGATGA CAGAGCAGGA TCAACAACGG 
CTCATCGATG ACCACTTTCT GTTTGATAAG CCAGTGTCCC CTTTACTAAC ATGCGCTGGG 
ATGGCCCGTG ACTGGCCTGA TGCCAGAGGA ATCTGGCATA ATTATGACAA GACATTTCTC 
ATCTGGATAA ATGAAGAAGA CCACACCAGG GTGATTTCAA TGGAGAAGGG AGGCAATATG 
AAACGCGTAT TTGAGCGATT CTGTCGTGGA CTAAAGGAAG TGGAACGACT AATCCAAGAA 
CGAGGCTGGG AGTTCATGTG GAATGAACGT CTAGGATACA TTTTGACTTG CCCCTCGAAC 
CTCGGAACTG GATTGCGAGC TGGTGTCCAC GTTAGGATCC CAAAGCTCAG CAAGGATCCA 
CGCTTTTCTA AGATCTTGGA GAACCTGCGG CTCCAGAAGC GTGGCACAGG AGGCGTGGAC 
ACTGCAGCAG TGGCCGACGT GTACGACATT TCCAACATAG ATCGGATCGG TAGATCTGAG 
GTTGAGCTTG TTCAGATTGT TATTGACGGA GTCAACTACC TGGTGGATTG TGAAAAGAAG 
CTGGAGAGAG GCCAGGACAT CAAGGTGCCG CCTCCCCTGC CTCAGTTTGG CAGGAAGTGA