Agap2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Agap2
Entrez Gene ID	65218
Full Name	ArfGAP with GTPase domain, ankyrin repeat and PH domain 2
Synonyms	Centg1,Pike
General protein information	Preferred Names ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 Names arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 AGAP-2 centaurin, gamma 1 cnt-g1 nuclear GTPase PIKE phosphatidylinositol 3-kinase enhancer
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q22
Summary	a nuclear GTPase that interacts with PI3K [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006241499.3	XP_006241561.1	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 isoform X1
NM_023026.2	NP_075415.2	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2
XM_006241500.3	XP_006241562.1	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 isoform X3
XM_008765421.2	XP_008763643.1	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 isoform X2

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	LOC100496997	XP_002933734.2
Bos taurus (cattle)	AGAP2	NP_001178968.1
Rattus norvegicus (Norway rat)	Agap2	NP_075415.2
Danio rerio (zebrafish)	agap2	NP_001073487.1
Pan troglodytes (chimpanzee)	AGAP2	XP_509171.3
Mus musculus (house mouse)	Agap2	NP_001028435.1
Macaca mulatta (Rhesus monkey)	AGAP2	XP_001116285.1
Homo sapiens (human)	AGAP2	NP_001116244.1

Gene Ontology
Bibliography

Related articles in PubMed

Reduced expression of E-cadherin and p120-catenin and elevated expression of PLC-γ1 and PIKE are associated with aggressiveness of oral squamous cell carcinoma.
Jiang Y, Liao L, Shrestha C, Ji S, Chen Y, Peng J, Wang L, Liao E, Xie Z
International journal of clinical and experimental pathology8(8)9042-51(2015)

PIKE is essential for oligodendroglia development and CNS myelination.
Chan CB, Liu X, Zhao L, Liu G, Lee CW, Feng Y, Ye K
Proceedings of the National Academy of Sciences of the United States of America111(5)1993-8(2014 Feb)

RACK1 promotes neurite outgrowth by scaffolding AGAP2 to FAK.
Dwane S, Durack E, O'Connor R, Kiely PA
Cellular signalling26(1)9-18(2014 Jan)

Essential role of PIKE GTPases in neuronal protection against excitotoxic insults.
Chan CB, Chen Y, Liu X, Papale L, Escayg A, Mei L, Ye K
Advances in biological regulation52(1)66-76(2012 Jan)

PIKE-mediated PI3-kinase activity is required for AMPA receptor surface expression.
Chan CB, Chen Y, Liu X, Tang X, Lee CW, Mei L, Ye K
The EMBO journal30(20)4274-86(2011 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Agap2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Agap2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa30280	XM_006241500.3 Latest version!	Rattus norvegicus ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 (Agap2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ORa43643	XM_008765421.2 Latest version!	Rattus norvegicus ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 (Agap2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ORa12708	NM_023026.2 Latest version!	Rattus norvegicus ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 (Agap2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ORa30279	XM_006241499.3 Latest version!	Rattus norvegicus ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 (Agap2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Agap2 ( XM_006241500.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006241562.1 Rattus norvegicus ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 (Agap2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa30280
Clone ID Related Accession (Same CDS sequence)	XM_006241500.3
Accession Version	XM_006241500.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2511bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006241500.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATGCCC AGAGGCAGTT GGCTATAGTC ACAGTAAGAG CAGAAGTCAG GCGACATGAG 
GTGGCCAAGC AAGCTCTAAG CCGACTCAGG AAGCTGGCAG AGAGAGTGGG TGACCCTGAA 
CTCCGAGCTG GCATCCAGGC CTCACTGGAC AGCATACGAG AGGCTGTGGT CAATAGTCAG 
GAATGGACTT TGAGCCGATC AATTCCCGAA CTGCGCCTGG GTGTGCTGGG TGATGTCAGG 
AGTGGGAAGT CATCCCTCAT CCACAGATTC CTCACGGGTT CATACCAGGT GCTGGAGAAG 
CCTGAGAGTG AGCAGTACAA GAAGGAGATG CTGGTGGATG GGCAGACCCA TCTGGTGCTG 
ATCCGAGAGG AAGCAGGGGC ACCGGATGCC AAGTTCTCAG GCTGGGCAGA TGCTGTGATC 
TTCGTCTTCA GCCTGGAGGA TGAGAGCAGC TTCCAGGCTG TGAGCCATCT CCATGGGCAG 
CTGATCTCCC TCCGGGGGGA GGGACGAGGA GGTCTGGCCC TTGCCCTGGT TGGGACCCAA 
GACAGGATCA GCGCATCGTC CCCTCGAGTG GTGGGTGATG CTCGAGCCCG GGCATTGTGT 
ACCGACATGA AGCGCTGCAG CTACTATGAG ACCTGTGCAA CCTATGGGCT CAATGTGGAC 
CGGGTCTTCC AGGAGGTGGC CCAGAAGGTG GTGACCTTGC GCAAACAGCA GCAACTCCTG 
GCGGCTTGCA AGTCCCTGCC CAGCTCACCG AGCCATTCAG CTGCTTCTAC TCCTGTAGCT 
GGGCAGGCTA GTAATGGGGG CCACACTAGC GACTACTCTT CTTCCCTCCC ATCTTCACCC 
AATGTTGGAC ACCGGGAGCT CCGGGCTGAG GCAGCTGCGG TGGCTGGGTT GAGCACCCCA 
GGGTCCTTAC ATCGAGCAGC CAAGCGGCGG ACCAGCCTTT TTGCGAACCG TCGAGGCAGT 
GATTCTGAGA AACGGAGCTT AGACAGTCGG GGCGAGACAA CAGGGAGTGG GCGAGCCATC 
CCCATTAAAC AGAGTTTCCT GCTAAAGCGA AGTGGCAATT CCTTGAACAA AGAGTGGAAG 
AAGAAATACG TGACCTTGTC CAGCAATGGC TTTCTGCTCT ACCACCCTAG CATTAATGAT 
TACATCCACA GTACACACGG CAAAGAGATG GACTTGTTAC GAACAACAGT CAAAGTCCCA 
GGCAAGCGGC CCCCAAGGGC TATCTCTGCT TTCGGCCCCT CAGCCAGCAT CAATGGGTTG 
GTCAAGGATA TGAGCACTGT CCAGATGGGT GAAGGCCCTG AAGCCTCAAC TCCAATGCCA 
AGCCCCAGTC CCAGCCCCAG CTCCCTGCAG CTCCCAACAG ACCAAACATC CAAGCACCTG 
TTGAAGCCAG ACCGGAATTT GGCCCGTGCC CTTAGCACCG ACTGTACCCC ATCTGGAGAC 
CTGAGTCCCC TGAGTCGGGA ACCCCCACCT TCTCCCATGG TGAAGAAGCA GAGGAGGAAG 
AAATTGTCCA CACCATCTAA GACTGAAGGC TCAGCTGTGC AGGCTGAAGA GGAAAACTTT 
GAGTTCCTGA TCGTGTCCAG CACTGGTCAG ACGTGGCACT TTGAGGCAGC CAGCTTCGAG 
GAGAGGGATG CCTGGGTTCA GGCCATTGAG AGTCAGATCC TGGCCAGTCT GCAATGCTGT 
GAGAGCAGCA AGGTCAAGCT GCGCACTGAC AGCCAAAGTG AAGCCGTGGC CATCCAGGCG 
ATCCGCAACG CAAAGGGAAA CTCGACCTGT GTGGACTGTG GAGCCCCCAA CCCCACGTGG 
GCCAGTTTGA ATCTGGGCGC ACTCATCTGC ATCGAATGTT CTGGCATCCA CCGGAACCTG 
GGCACGCACC TGTCTCGAGT GCGTTCGCTG GACTTGGATG ACTGGCCGCG GGAGCTGACC 
CTGGTGCTGA CGGCCATTGG CAACGACACG GCCAATCGCG TGTGGGAGAG CGACACTCGG 
GGCCGTGCCA AGCCCACGCG GGACTCTTCG AGGGAGGAGC GTGAGTCGTG GATTCGGGCC 
AAGTACGAAC AGCTGCTGTT CCTGGCGCCT CTGGGCACCA CGGAGGAGCC GCTGGGCCGC 
CAACTGTGGG CCGCTGTGGA GGCCCAGGAC GTGGCCGCCG TTCTGCTGCT TCTGGCCCAC 
GCGCGACATG GGCCGCTGGA CACCAGCGTG GAGGACCCTC AGCTCCGCTC GCCGCTGCAC 
TTGGCGGCCG AGCTCGCCCA CGTTGTTATT ACACAGCTGC TGTTGTGGTA TGGCGCAGAC 
GTGGCGGCCC GCGATGCGCA GGGCCGCACA GCATTGTTCT ACGCCCGCCA GGCTGGCAGC 
CAGCTATGTG CAGACATCCT CCTCCAGCAC GGCTGTCCGG GAGAGGGCGG CAGTACAGCC 
ACCACGCCCA GCGCGGCCAC CACCCCCAGC ATTACCGCCA CGCCCAGCCC GAGGCGCCGG 
AGCAGTGCTG CCAGCTTGGG TCGCGTGGAC ACCACGATCG CGCTGGTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006241562.1
CDS	344..2854
Translation	MHAQRQLAIVTVRAEVRRHEVAKQALSRLRKLAERVGDPELRAGIQASLDSIREAVVNSQEWTLSRSIPELRLGVLGDVRSGKSSLIHRFLTGSYQVLEKPESEQYKKEMLVDGQTHLVLIREEAGAPDAKFSGWADAVIFVFSLEDESSFQAVSHLHGQLISLRGEGRGGLALALVGTQDRISASSPRVVGDARARALCTDMKRCSYYETCATYGLNVDRVFQEVAQKVVTLRKQQQLLAACKSLPSSPSHSAASTPVAGQASNGGHTSDYSSSLPSSPNVGHRELRAEAAAVAGLSTPGSLHRAAKRRTSLFANRRGSDSEKRSLDSRGETTGSGRAIPIKQSFLLKRSGNSLNKEWKKKYVTLSSNGFLLYHPSINDYIHSTHGKEMDLLRTTVKVPGKRPPRAISAFGPSASINGLVKDMSTVQMGEGPEASTPMPSPSPSPSSLQLPTDQTSKHLLKPDRNLARALSTDCTPSGDLSPLSREPPPSPMVKKQRRKKLSTPSKTEGSAVQAEEENFEFLIVSSTGQTWHFEAASFEERDAWVQAIESQILASLQCCESSKVKLRTDSQSEAVAIQAIRNAKGNSTCVDCGAPNPTWASLNLGALICIECSGIHRNLGTHLSRVRSLDLDDWPRELTLVLTAIGNDTANRVWESDTRGRAKPTRDSSREERESWIRAKYEQLLFLAPLGTTEEPLGRQLWAAVEAQDVAAVLLLLAHARHGPLDTSVEDPQLRSPLHLAAELAHVVITQLLLWYGADVAARDAQGRTALFYARQAGSQLCADILLQHGCPGEGGSTATTPSAATTPSITATPSPRRRSSAASLGRVDTTIALV

Target ORF information:

RefSeq Version	XM_006241500.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 (Agap2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006241500.3

ORF Insert Sequence:

ATGCATGCCC AGAGGCAGTT GGCTATAGTC ACAGTAAGAG CAGAAGTCAG GCGACATGAG 
GTGGCCAAGC AAGCTCTAAG CCGACTCAGG AAGCTGGCAG AGAGAGTGGG TGACCCTGAA 
CTCCGAGCTG GCATCCAGGC CTCACTGGAC AGCATACGAG AGGCTGTGGT CAATAGTCAG 
GAATGGACTT TGAGCCGATC AATTCCCGAA CTGCGCCTGG GTGTGCTGGG TGATGTCAGG 
AGTGGGAAGT CATCCCTCAT CCACAGATTC CTCACGGGTT CATACCAGGT GCTGGAGAAG 
CCTGAGAGTG AGCAGTACAA GAAGGAGATG CTGGTGGATG GGCAGACCCA TCTGGTGCTG 
ATCCGAGAGG AAGCAGGGGC ACCGGATGCC AAGTTCTCAG GCTGGGCAGA TGCTGTGATC 
TTCGTCTTCA GCCTGGAGGA TGAGAGCAGC TTCCAGGCTG TGAGCCATCT CCATGGGCAG 
CTGATCTCCC TCCGGGGGGA GGGACGAGGA GGTCTGGCCC TTGCCCTGGT TGGGACCCAA 
GACAGGATCA GCGCATCGTC CCCTCGAGTG GTGGGTGATG CTCGAGCCCG GGCATTGTGT 
ACCGACATGA AGCGCTGCAG CTACTATGAG ACCTGTGCAA CCTATGGGCT CAATGTGGAC 
CGGGTCTTCC AGGAGGTGGC CCAGAAGGTG GTGACCTTGC GCAAACAGCA GCAACTCCTG 
GCGGCTTGCA AGTCCCTGCC CAGCTCACCG AGCCATTCAG CTGCTTCTAC TCCTGTAGCT 
GGGCAGGCTA GTAATGGGGG CCACACTAGC GACTACTCTT CTTCCCTCCC ATCTTCACCC 
AATGTTGGAC ACCGGGAGCT CCGGGCTGAG GCAGCTGCGG TGGCTGGGTT GAGCACCCCA 
GGGTCCTTAC ATCGAGCAGC CAAGCGGCGG ACCAGCCTTT TTGCGAACCG TCGAGGCAGT 
GATTCTGAGA AACGGAGCTT AGACAGTCGG GGCGAGACAA CAGGGAGTGG GCGAGCCATC 
CCCATTAAAC AGAGTTTCCT GCTAAAGCGA AGTGGCAATT CCTTGAACAA AGAGTGGAAG 
AAGAAATACG TGACCTTGTC CAGCAATGGC TTTCTGCTCT ACCACCCTAG CATTAATGAT 
TACATCCACA GTACACACGG CAAAGAGATG GACTTGTTAC GAACAACAGT CAAAGTCCCA 
GGCAAGCGGC CCCCAAGGGC TATCTCTGCT TTCGGCCCCT CAGCCAGCAT CAATGGGTTG 
GTCAAGGATA TGAGCACTGT CCAGATGGGT GAAGGCCCTG AAGCCTCAAC TCCAATGCCA 
AGCCCCAGTC CCAGCCCCAG CTCCCTGCAG CTCCCAACAG ACCAAACATC CAAGCACCTG 
TTGAAGCCAG ACCGGAATTT GGCCCGTGCC CTTAGCACCG ACTGTACCCC ATCTGGAGAC 
CTGAGTCCCC TGAGTCGGGA ACCCCCACCT TCTCCCATGG TGAAGAAGCA GAGGAGGAAG 
AAATTGTCCA CACCATCTAA GACTGAAGGC TCAGCTGTGC AGGCTGAAGA GGAAAACTTT 
GAGTTCCTGA TCGTGTCCAG CACTGGTCAG ACGTGGCACT TTGAGGCAGC CAGCTTCGAG 
GAGAGGGATG CCTGGGTTCA GGCCATTGAG AGTCAGATCC TGGCCAGTCT GCAATGCTGT 
GAGAGCAGCA AGGTCAAGCT GCGCACTGAC AGCCAAAGTG AAGCCGTGGC CATCCAGGCG 
ATCCGCAACG CAAAGGGAAA CTCGACCTGT GTGGACTGTG GAGCCCCCAA CCCCACGTGG 
GCCAGTTTGA ATCTGGGCGC ACTCATCTGC ATCGAATGTT CTGGCATCCA CCGGAACCTG 
GGCACGCACC TGTCTCGAGT GCGTTCGCTG GACTTGGATG ACTGGCCGCG GGAGCTGACC 
CTGGTGCTGA CGGCCATTGG CAACGACACG GCCAATCGCG TGTGGGAGAG CGACACTCGG 
GGCCGTGCCA AGCCCACGCG GGACTCTTCG AGGGAGGAGC GTGAGTCGTG GATTCGGGCC 
AAGTACGAAC AGCTGCTGTT CCTGGCGCCT CTGGGCACCA CGGAGGAGCC GCTGGGCCGC 
CAACTGTGGG CCGCTGTGGA GGCCCAGGAC GTGGCCGCCG TTCTGCTGCT TCTGGCCCAC 
GCGCGACATG GGCCGCTGGA CACCAGCGTG GAGGACCCTC AGCTCCGCTC GCCGCTGCAC 
TTGGCGGCCG AGCTCGCCCA CGTTGTTATT ACACAGCTGC TGTTGTGGTA TGGCGCAGAC 
GTGGCGGCCC GCGATGCGCA GGGCCGCACA GCATTGTTCT ACGCCCGCCA GGCTGGCAGC 
CAGCTATGTG CAGACATCCT CCTCCAGCAC GGCTGTCCGG GAGAGGGCGG CAGTACAGCC 
ACCACGCCCA GCGCGGCCAC CACCCCCAGC ATTACCGCCA CGCCCAGCCC GAGGCGCCGG 
AGCAGTGCTG CCAGCTTGGG TCGCGTGGAC ACCACGATCG CGCTGGTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agap2 ( XM_008765421.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008763643.1 Rattus norvegicus ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 (Agap2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa43643
Clone ID Related Accession (Same CDS sequence)	XM_008765421.2
Accession Version	XM_008765421.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2571bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008765421.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATGCCC AGAGGCAGTT GGCTATAGTC ACAGTAAGAG CAGAAGTCAG GCGACATGAG 
GTGGCCAAGC AAGCTCTAAG CCGACTCAGG AAGCTGGCAG AGAGAGTGGG TGACCCTGAA 
CTCCGAGCTG GCATCCAGGC CTCACTGGAC AGCATACGAG AGGCTGTGGT CAATAGTCAG 
GAATGGACTT TGAGCCGATC AATTCCCGAA CTGCGCCTGG GTGTGCTGGG TGATGTCAGG 
AGTGGGAAGT CATCCCTCAT CCACAGATTC CTCACGGGTT CATACCAGGT GCTGGAGAAG 
CCTGAGAGTG AGCAGTACAA GAAGGAGATG CTGGTGGATG GGCAGACCCA TCTGGTGCTG 
ATCCGAGAGG AAGCAGGGGC ACCGGATGCC AAGTTCTCAG GCTGGGCAGA TGCTGTGATC 
TTCGTCTTCA GCCTGGAGGA TGAGAGCAGC TTCCAGGCTG TGAGCCATCT CCATGGGCAG 
CTGATCTCCC TCCGGGGGGA GGGACGAGGA GGTCTGGCCC TTGCCCTGGT TGGGACCCAA 
GACAGGATCA GCGCATCGTC CCCTCGAGTG GTGGGTGATG CTCGAGCCCG GGCATTGTGT 
ACCGACATGA AGCGCTGCAG CTACTATGAG ACCTGTGCAA CCTATGGGCT CAATGTGGAC 
CGGGTCTTCC AGGAGGTGGC CCAGAAGGTG GTGACCTTGC GCAAACAGCA GCAACTCCTG 
GCGGCTTGCA AGTCCCTGCC CAGCTCACCG AGCCATTCAG CTGCTTCTAC TCCTGTAGCT 
GGGCAGGCTA GTAATGGGGG CCACACTAGC GACTACTCTT CTTCCCTCCC ATCTTCACCC 
AATGTTGGAC ACCGGGAGCT CCGGGCTGAG GCAGCTGCGG TGGCTGGGTT GAGCACCCCA 
GGGTCCTTAC ATCGAGCAGC CAAGCGGCGG ACCAGCCTTT TTGCGAACCG TCGAGGCAGT 
GATTCTGAGA AACGGAGCTT AGACAGTCGG GGCGAGACAA CAGGGAGTGG GCGAGCCATC 
CCCATTAAAC AGAGTTTCCT GCTAAAGCGA AGTGGCAATT CCTTGAACAA AGAGTGGAAG 
AAGAAATACG TGACCTTGTC CAGCAATGGC TTTCTGCTCT ACCACCCTAG CATTAATGAT 
TACATCCACA GTACACACGG CAAAGAGATG GACTTGTTAC GAACAACAGT CAAAGTCCCA 
GGCAAGCGGC CCCCAAGGGC TATCTCTGCT TTCGGCCCCT CAGCCAGCAT CAATGGGTTG 
GTCAAGGATA TGAGCACTGT CCAGATGGGT GAAGGCCCTG AAGCCTCAAC TCCAATGCCA 
AGCCCCAGTC CCAGCCCCAG CTCCCTGCAG CTCCCAACAG ACCAAACATC CAAGCACCTG 
TTGAAGCCAG ACCGGAATTT GGCCCGTGCC CTTAGCACCG ACTGTACCCC ATCTGGAGAC 
CTGAGTCCCC TGAGTCGGGA ACCCCCACCT TCTCCCATGG TGAAGAAGCA GAGGAGGAAG 
AAATTGTCCA CACCATCTAA GACTGAAGGC TCAGCTGTGC AGGCTGAAGC CAAGCGCAAA 
ATGTGGAAAC TAAAATCCTT TGGTAGTTTA AGAAATATTT ATAAAGCAGA GGAAAACTTT 
GAGTTCCTGA TCGTGTCCAG CACTGGTCAG ACGTGGCACT TTGAGGCAGC CAGCTTCGAG 
GAGAGGGATG CCTGGGTTCA GGCCATTGAG AGTCAGATCC TGGCCAGTCT GCAATGCTGT 
GAGAGCAGCA AGGTCAAGCT GCGCACTGAC AGCCAAAGTG AAGCCGTGGC CATCCAGGCG 
ATCCGCAACG CAAAGGGAAA CTCGACCTGT GTGGACTGTG GAGCCCCCAA CCCCACGTGG 
GCCAGTTTGA ATCTGGGCGC ACTCATCTGC ATCGAATGTT CTGGCATCCA CCGGAACCTG 
GGCACGCACC TGTCTCGAGT GCGTTCGCTG GACTTGGATG ACTGGCCGCG GGAGCTGACC 
CTGGTGCTGA CGGCCATTGG CAACGACACG GCCAATCGCG TGTGGGAGAG CGACACTCGG 
GGCCGTGCCA AGCCCACGCG GGACTCTTCG AGGGAGGAGC GTGAGTCGTG GATTCGGGCC 
AAGTACGAAC AGCTGCTGTT CCTGGCGCCT CTGGGCACCA CGGAGGAGCC GCTGGGCCGC 
CAACTGTGGG CCGCTGTGGA GGCCCAGGAC GTGGCCGCCG TTCTGCTGCT TCTGGCCCAC 
GCGCGACATG GGCCGCTGGA CACCAGCGTG GAGGACCCTC AGCTCCGCTC GCCGCTGCAC 
TTGGCGGCCG AGCTCGCCCA CGTTGTTATT ACACAGCTGC TGTTGTGGTA TGGCGCAGAC 
GTGGCGGCCC GCGATGCGCA GGGCCGCACA GCATTGTTCT ACGCCCGCCA GGCTGGCAGC 
CAGCTATGTG CAGACATCCT CCTCCAGCAC GGCTGTCCGG GAGAGGGCGG CAGTACAGCC 
ACCACGCCCA GCGCGGCCAC CACCCCCAGC ATTACCGCCA CGCCCAGCCC GAGGCGCCGG 
AGCAGTGCTG CCAGCTTGGG TCGCGTGGAC ACCACGATCG CGCTGGTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008763643.1
CDS	347..2917
Translation	MHAQRQLAIVTVRAEVRRHEVAKQALSRLRKLAERVGDPELRAGIQASLDSIREAVVNSQEWTLSRSIPELRLGVLGDVRSGKSSLIHRFLTGSYQVLEKPESEQYKKEMLVDGQTHLVLIREEAGAPDAKFSGWADAVIFVFSLEDESSFQAVSHLHGQLISLRGEGRGGLALALVGTQDRISASSPRVVGDARARALCTDMKRCSYYETCATYGLNVDRVFQEVAQKVVTLRKQQQLLAACKSLPSSPSHSAASTPVAGQASNGGHTSDYSSSLPSSPNVGHRELRAEAAAVAGLSTPGSLHRAAKRRTSLFANRRGSDSEKRSLDSRGETTGSGRAIPIKQSFLLKRSGNSLNKEWKKKYVTLSSNGFLLYHPSINDYIHSTHGKEMDLLRTTVKVPGKRPPRAISAFGPSASINGLVKDMSTVQMGEGPEASTPMPSPSPSPSSLQLPTDQTSKHLLKPDRNLARALSTDCTPSGDLSPLSREPPPSPMVKKQRRKKLSTPSKTEGSAVQAEAKRKMWKLKSFGSLRNIYKAEENFEFLIVSSTGQTWHFEAASFEERDAWVQAIESQILASLQCCESSKVKLRTDSQSEAVAIQAIRNAKGNSTCVDCGAPNPTWASLNLGALICIECSGIHRNLGTHLSRVRSLDLDDWPRELTLVLTAIGNDTANRVWESDTRGRAKPTRDSSREERESWIRAKYEQLLFLAPLGTTEEPLGRQLWAAVEAQDVAAVLLLLAHARHGPLDTSVEDPQLRSPLHLAAELAHVVITQLLLWYGADVAARDAQGRTALFYARQAGSQLCADILLQHGCPGEGGSTATTPSAATTPSITATPSPRRRSSAASLGRVDTTIALV

Target ORF information:

RefSeq Version	XM_008765421.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 (Agap2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008765421.2

ORF Insert Sequence:

ATGCATGCCC AGAGGCAGTT GGCTATAGTC ACAGTAAGAG CAGAAGTCAG GCGACATGAG 
GTGGCCAAGC AAGCTCTAAG CCGACTCAGG AAGCTGGCAG AGAGAGTGGG TGACCCTGAA 
CTCCGAGCTG GCATCCAGGC CTCACTGGAC AGCATACGAG AGGCTGTGGT CAATAGTCAG 
GAATGGACTT TGAGCCGATC AATTCCCGAA CTGCGCCTGG GTGTGCTGGG TGATGTCAGG 
AGTGGGAAGT CATCCCTCAT CCACAGATTC CTCACGGGTT CATACCAGGT GCTGGAGAAG 
CCTGAGAGTG AGCAGTACAA GAAGGAGATG CTGGTGGATG GGCAGACCCA TCTGGTGCTG 
ATCCGAGAGG AAGCAGGGGC ACCGGATGCC AAGTTCTCAG GCTGGGCAGA TGCTGTGATC 
TTCGTCTTCA GCCTGGAGGA TGAGAGCAGC TTCCAGGCTG TGAGCCATCT CCATGGGCAG 
CTGATCTCCC TCCGGGGGGA GGGACGAGGA GGTCTGGCCC TTGCCCTGGT TGGGACCCAA 
GACAGGATCA GCGCATCGTC CCCTCGAGTG GTGGGTGATG CTCGAGCCCG GGCATTGTGT 
ACCGACATGA AGCGCTGCAG CTACTATGAG ACCTGTGCAA CCTATGGGCT CAATGTGGAC 
CGGGTCTTCC AGGAGGTGGC CCAGAAGGTG GTGACCTTGC GCAAACAGCA GCAACTCCTG 
GCGGCTTGCA AGTCCCTGCC CAGCTCACCG AGCCATTCAG CTGCTTCTAC TCCTGTAGCT 
GGGCAGGCTA GTAATGGGGG CCACACTAGC GACTACTCTT CTTCCCTCCC ATCTTCACCC 
AATGTTGGAC ACCGGGAGCT CCGGGCTGAG GCAGCTGCGG TGGCTGGGTT GAGCACCCCA 
GGGTCCTTAC ATCGAGCAGC CAAGCGGCGG ACCAGCCTTT TTGCGAACCG TCGAGGCAGT 
GATTCTGAGA AACGGAGCTT AGACAGTCGG GGCGAGACAA CAGGGAGTGG GCGAGCCATC 
CCCATTAAAC AGAGTTTCCT GCTAAAGCGA AGTGGCAATT CCTTGAACAA AGAGTGGAAG 
AAGAAATACG TGACCTTGTC CAGCAATGGC TTTCTGCTCT ACCACCCTAG CATTAATGAT 
TACATCCACA GTACACACGG CAAAGAGATG GACTTGTTAC GAACAACAGT CAAAGTCCCA 
GGCAAGCGGC CCCCAAGGGC TATCTCTGCT TTCGGCCCCT CAGCCAGCAT CAATGGGTTG 
GTCAAGGATA TGAGCACTGT CCAGATGGGT GAAGGCCCTG AAGCCTCAAC TCCAATGCCA 
AGCCCCAGTC CCAGCCCCAG CTCCCTGCAG CTCCCAACAG ACCAAACATC CAAGCACCTG 
TTGAAGCCAG ACCGGAATTT GGCCCGTGCC CTTAGCACCG ACTGTACCCC ATCTGGAGAC 
CTGAGTCCCC TGAGTCGGGA ACCCCCACCT TCTCCCATGG TGAAGAAGCA GAGGAGGAAG 
AAATTGTCCA CACCATCTAA GACTGAAGGC TCAGCTGTGC AGGCTGAAGC CAAGCGCAAA 
ATGTGGAAAC TAAAATCCTT TGGTAGTTTA AGAAATATTT ATAAAGCAGA GGAAAACTTT 
GAGTTCCTGA TCGTGTCCAG CACTGGTCAG ACGTGGCACT TTGAGGCAGC CAGCTTCGAG 
GAGAGGGATG CCTGGGTTCA GGCCATTGAG AGTCAGATCC TGGCCAGTCT GCAATGCTGT 
GAGAGCAGCA AGGTCAAGCT GCGCACTGAC AGCCAAAGTG AAGCCGTGGC CATCCAGGCG 
ATCCGCAACG CAAAGGGAAA CTCGACCTGT GTGGACTGTG GAGCCCCCAA CCCCACGTGG 
GCCAGTTTGA ATCTGGGCGC ACTCATCTGC ATCGAATGTT CTGGCATCCA CCGGAACCTG 
GGCACGCACC TGTCTCGAGT GCGTTCGCTG GACTTGGATG ACTGGCCGCG GGAGCTGACC 
CTGGTGCTGA CGGCCATTGG CAACGACACG GCCAATCGCG TGTGGGAGAG CGACACTCGG 
GGCCGTGCCA AGCCCACGCG GGACTCTTCG AGGGAGGAGC GTGAGTCGTG GATTCGGGCC 
AAGTACGAAC AGCTGCTGTT CCTGGCGCCT CTGGGCACCA CGGAGGAGCC GCTGGGCCGC 
CAACTGTGGG CCGCTGTGGA GGCCCAGGAC GTGGCCGCCG TTCTGCTGCT TCTGGCCCAC 
GCGCGACATG GGCCGCTGGA CACCAGCGTG GAGGACCCTC AGCTCCGCTC GCCGCTGCAC 
TTGGCGGCCG AGCTCGCCCA CGTTGTTATT ACACAGCTGC TGTTGTGGTA TGGCGCAGAC 
GTGGCGGCCC GCGATGCGCA GGGCCGCACA GCATTGTTCT ACGCCCGCCA GGCTGGCAGC 
CAGCTATGTG CAGACATCCT CCTCCAGCAC GGCTGTCCGG GAGAGGGCGG CAGTACAGCC 
ACCACGCCCA GCGCGGCCAC CACCCCCAGC ATTACCGCCA CGCCCAGCCC GAGGCGCCGG 
AGCAGTGCTG CCAGCTTGGG TCGCGTGGAC ACCACGATCG CGCTGGTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agap2 ( NM_023026.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_075415.2 Rattus norvegicus ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 (Agap2), mRNA.

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CloneID	ORa12708
Clone ID Related Accession (Same CDS sequence)	NM_023026.2
Accession Version	NM_023026.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3561bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577289600000
Organism	Rattus norvegicus(Norway rat)
Product	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF280816.1, AY128688.1 and AABR07073309.1. On or before Sep 25, 2009 this sequence version replaced XM_001054911.1, XM_576238.2, NM_023026.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY128688.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGAGCCGGG GCGCGGGCGC GCTTCAGCGC CGGACAACGA CCTACCTCAT CTCGCTGACC 
CTGGTCAAGC TCGAGTCGGT ACCTCCGCCG CCGCCTTCTC CGTCTGCAGC TGCGGTCGGC 
GCCCCTGGGG CCAGAGGCTC GGAGCCCCGA GATCCTGGCA GCCCCCGAGG CGCGGAGGAA 
CCCGGCAAGA AGCGGCACGA GCGTCTCTTC CACCGGCAGG ATGCGCTGTG GATCAGTACC 
AGCAGCGCGG GCGCCGGGGG CGCGGAGCCC CCCGCGCTGT CCCCGGCTCC GGCCAGTCCG 
GCTCGCCCGG TCTCCCCGGC TCCGGGCCGC CGACTGTCCC TCTGGGCCGC CCCTCCGGGA 
CCCCCGCTCT CCGGGGGGCT GAGCCCGGAC TCCAAGCCGG GGGGCGCCCC CTCTTCCTCT 
CGGCGCCCTC TACTCAGCAG CCCGAGCTGG GGGGGTCCGG AACCTGAAGG CCGGACGGGT 
GGCGGCGTCC CGGGCTCGTC CTCCCCGCAT CCTGGCACCG GTAGCCGAAG GCTCAAGGTG 
GCGCCTCCTC CGCCAGCTCC CAAGCCTTTC AAGACCGTGA CCACTAGTGG AGCCAAAGCC 
GGCGGGGGCA AGGGCGCGGG TAGCCGCCTG TCATGGCCCG AAAGCGAGGG CAAACCCAGG 
GTCAAGGGGT CAAAGAGCAC CGCCGGGACT GGAGCCTCTG CCGTTGCGGC CGGCGGGGGA 
GGGAGCGCTG CAGTCACGAC CTCTGGTGGG GTCGGTGCTG GGGCTGGAGC GAGAGGAAAA 
TTGTCCCCTC GGAAAGGCAA GAGTAAGACC TTGGACAATA GTGACTTGCA CCCAGGACCC 
AGTGCCGGTT CTCCTCCGCT CACCGTGCCA GCAATCCCAG TTCCAGCTAC TTCTGTCACT 
GCCGCCTCCA CGCAGCCCCT CGGGCCTGCA CCCCCTATCA CTCTGGAGCC ACCAGCTCCG 
GGGCTAAAAA GGGGCCGGGA GGGCGGCCGA GCGTCCACTC GTGACCGCAA GATGCTCAAG 
TTTATCAGCG GTATCTTCAC CAAGAGCACA GGAGGGCCTC CCGGTCCCGG GCCCCTTCCC 
GGACCCCAAG GCTTGTCTTC CAGCAGCGGG TCCAGGGAGC TGCTGGGCGC AGAGCTACGC 
GCCTCCCCTA AGGCTGTGGT CAATAGTCAG GAATGGACTT TGAGCCGATC AATTCCCGAA 
CTGCGCCTGG GTGTGCTGGG TGATGTCAGG AGTGGGAAGT CATCCCTCAT CCACAGATTC 
CTCACGGGTT CATACCAGGT GCTGGAGAAG CCTGAGAGTG AGCAGTACAA GAAGGAGATG 
CTGGTGGATG GGCAGACCCA TCTGGTGCTG ATCCGAGAGG AAGCAGGGGC ACCGGATGCC 
AAGTTCTCAG GCTGGGCAGA TGCTGTGATC TTCGTCTTCA GCCTGGAGGA TGAGAGCAGC 
TTCCAGGCTG TGAGCCATCT CCATGGGCAG CTGATCTCCC TCCGGGGGGA GGGACGAGGA 
GGTCTGGCCC TTGCCCTGGT TGGGACCCAA GACAGGATCA GCGCATCGTC CCCTCGAGTG 
GTGGGTGATG CTCGAGCCCG GGCATTGTGT ACCGACATGA AGCGCTGCAG CTACTATGAG 
ACCTGTGCAA CCTATGGGCT CAATGTGGAC CGGGTCTTCC AGGAGGTGGC CCAGAAGGTG 
GTGACCTTGC GCAAACAGCA GCAACTCCTG GCGGCTTGCA AGTCCCTGCC CAGCTCACCG 
AGCCATTCAG CTGCTTCTAC TCCTGTAGCT GGGCAGGCTA GTAATGGGGG CCACACTAGC 
GACTACTCTT CTTCCCTCCC ATCTTCACCC AATGTTGGAC ACCGGGAGCT CCGGGCTGAG 
GCAGCTGCGG TGGCTGGGTT GAGCACCCCA GGGTCCTTAC ATCGAGCAGC CAAGCGGCGG 
ACCAGCCTTT TTGCGAACCG TCGAGGCAGT GATTCTGAGA AACGGAGCTT AGACAGTCGG 
GGCGAGACAA CAGGGAGTGG GCGAGCCATC CCCATTAAAC AGAGTTTCCT GCTAAAGCGA 
AGTGGCAATT CCTTGAACAA AGAGTGGAAG AAGAAATACG TGACCTTGTC CAGCAATGGC 
TTTCTGCTCT ACCACCCTAG CATTAATGAT TACATCCACA GTACACACGG CAAAGAGATG 
GACTTGTTAC GAACAACAGT CAAAGTCCCA GGCAAGCGGC CCCCAAGGGC TATCTCTGCT 
TTCGGCCCCT CAGCCAGCAT CAATGGGTTG GTCAAGGATA TGAGCACTGT CCAGATGGGT 
GAAGGCCCTG AAGCCTCAAC TCCAATGCCA AGCCCCAGTC CCAGCCCCAG CTCCCTGCAG 
CTCCCAACAG ACCAAACATC CAAGCACCTG TTGAAGCCAG ACCGGAATTT GGCCCGTGCC 
CTTAGCACCG ACTGTACCCC ATCTGGAGAC CTGAGTCCCC TGAGTCGGGA ACCCCCACCT 
TCTCCCATGG TGAAGAAGCA GAGGAGGAAG AAATTGTCCA CACCATCTAA GACTGAAGGC 
TCAGCTGTGC AGGCTGAAGC CAAGCGCAAA ATGTGGAAAC TAAAATCCTT TGGTAGTTTA 
AGAAATATTT ATAAAGCAGA GGAAAACTTT GAGTTCCTGA TCGTGTCCAG CACTGGTCAG 
ACGTGGCACT TTGAGGCAGC CAGCTTCGAG GAGAGGGATG CCTGGGTTCA GGCCATTGAG 
AGTCAGATCC TGGCCAGTCT GCAATGCTGT GAGAGCAGCA AGGTCAAGCT GCGCACTGAC 
AGCCAAAGTG AAGCCGTGGC CATCCAGGCG ATCCGCAACG CAAAGGGAAA CTCGACCTGT 
GTGGACTGTG GAGCCCCCAA CCCCACGTGG GCCAGTTTGA ATCTGGGCGC ACTCATCTGC 
ATCGAATGTT CTGGCATCCA CCGGAACCTG GGCACGCACC TGTCTCGAGT GCGTTCGCTG 
GACTTGGATG ACTGGCCGCG GGAGCTGACC CTGGTGCTGA CGGCCATTGG CAACGACACG 
GCCAATCGCG TGTGGGAGAG CGACACTCGG GGCCGTGCCA AGCCCACGCG GGACTCTTCG 
AGGGAGGAGC GTGAGTCGTG GATTCGGGCC AAGTACGAAC AGCTGCTGTT CCTGGCGCCT 
CTGGGCACCA CGGAGGAGCC GCTGGGCCGC CAACTGTGGG CCGCTGTGGA GGCCCAGGAC 
GTGGCCGCCG TTCTGCTGCT TCTGGCCCAC GCGCGACATG GGCCGCTGGA CACCAGCGTG 
GAGGACCCTC AGCTCCGCTC GCCGCTGCAC TTGGCGGCCG AGCTCGCCCA CGTTGTTATT 
ACACAGCTGC TGTTGTGGTA TGGCGCAGAC GTGGCGGCCC GCGATGCGCA GGGCCGCACA 
GCATTGTTCT ACGCCCGCCA GGCTGGCAGC CAGCTATGTG CAGACATCCT CCTCCAGCAC 
GGCTGTCCGG GAGAGGGCGG CAGTACAGCC ACCACGCCCA GCGCGGCCAC CACCCCCAGC 
ATTACCGCCA CGCCCAGCCC GAGGCGCCGG AGCAGTGCTG CCAGCTTGGG TCGCGTGGAC 
ACCACGATCG CGCTGGTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_075415.2
CDS	1..3561
Translation	MSRGAGALQRRTTTYLISLTLVKLESVPPPPPSPSAAAVGAPGARGSEPRDPGSPRGAEEPGKKRHERLFHRQDALWISTSSAGAGGAEPPALSPAPASPARPVSPAPGRRLSLWAAPPGPPLSGGLSPDSKPGGAPSSSRRPLLSSPSWGGPEPEGRTGGGVPGSSSPHPGTGSRRLKVAPPPPAPKPFKTVTTSGAKAGGGKGAGSRLSWPESEGKPRVKGSKSTAGTGASAVAAGGGGSAAVTTSGGVGAGAGARGKLSPRKGKSKTLDNSDLHPGPSAGSPPLTVPAIPVPATSVTAASTQPLGPAPPITLEPPAPGLKRGREGGRASTRDRKMLKFISGIFTKSTGGPPGPGPLPGPQGLSSSSGSRELLGAELRASPKAVVNSQEWTLSRSIPELRLGVLGDVRSGKSSLIHRFLTGSYQVLEKPESEQYKKEMLVDGQTHLVLIREEAGAPDAKFSGWADAVIFVFSLEDESSFQAVSHLHGQLISLRGEGRGGLALALVGTQDRISASSPRVVGDARARALCTDMKRCSYYETCATYGLNVDRVFQEVAQKVVTLRKQQQLLAACKSLPSSPSHSAASTPVAGQASNGGHTSDYSSSLPSSPNVGHRELRAEAAAVAGLSTPGSLHRAAKRRTSLFANRRGSDSEKRSLDSRGETTGSGRAIPIKQSFLLKRSGNSLNKEWKKKYVTLSSNGFLLYHPSINDYIHSTHGKEMDLLRTTVKVPGKRPPRAISAFGPSASINGLVKDMSTVQMGEGPEASTPMPSPSPSPSSLQLPTDQTSKHLLKPDRNLARALSTDCTPSGDLSPLSREPPPSPMVKKQRRKKLSTPSKTEGSAVQAEAKRKMWKLKSFGSLRNIYKAEENFEFLIVSSTGQTWHFEAASFEERDAWVQAIESQILASLQCCESSKVKLRTDSQSEAVAIQAIRNAKGNSTCVDCGAPNPTWASLNLGALICIECSGIHRNLGTHLSRVRSLDLDDWPRELTLVLTAIGNDTANRVWESDTRGRAKPTRDSSREERESWIRAKYEQLLFLAPLGTTEEPLGRQLWAAVEAQDVAAVLLLLAHARHGPLDTSVEDPQLRSPLHLAAELAHVVITQLLLWYGADVAARDAQGRTALFYARQAGSQLCADILLQHGCPGEGGSTATTPSAATTPSITATPSPRRRSSAASLGRVDTTIALV

Target ORF information:

RefSeq Version	NM_023026.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 (Agap2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_023026.2

ORF Insert Sequence:

ATGAGCCGGG GCGCGGGCGC GCTTCAGCGC CGGACAACGA CCTACCTCAT CTCGCTGACC 
CTGGTCAAGC TCGAGTCGGT ACCTCCGCCG CCGCCTTCTC CGTCTGCAGC TGCGGTCGGC 
GCCCCTGGGG CCAGAGGCTC GGAGCCCCGA GATCCTGGCA GCCCCCGAGG CGCGGAGGAA 
CCCGGCAAGA AGCGGCACGA GCGTCTCTTC CACCGGCAGG ATGCGCTGTG GATCAGTACC 
AGCAGCGCGG GCGCCGGGGG CGCGGAGCCC CCCGCGCTGT CCCCGGCTCC GGCCAGTCCG 
GCTCGCCCGG TCTCCCCGGC TCCGGGCCGC CGACTGTCCC TCTGGGCCGC CCCTCCGGGA 
CCCCCGCTCT CCGGGGGGCT GAGCCCGGAC TCCAAGCCGG GGGGCGCCCC CTCTTCCTCT 
CGGCGCCCTC TACTCAGCAG CCCGAGCTGG GGGGGTCCGG AACCTGAAGG CCGGACGGGT 
GGCGGCGTCC CGGGCTCGTC CTCCCCGCAT CCTGGCACCG GTAGCCGAAG GCTCAAGGTG 
GCGCCTCCTC CGCCAGCTCC CAAGCCTTTC AAGACCGTGA CCACTAGTGG AGCCAAAGCC 
GGCGGGGGCA AGGGCGCGGG TAGCCGCCTG TCATGGCCCG AAAGCGAGGG CAAACCCAGG 
GTCAAGGGGT CAAAGAGCAC CGCCGGGACT GGAGCCTCTG CCGTTGCGGC CGGCGGGGGA 
GGGAGCGCTG CAGTCACGAC CTCTGGTGGG GTCGGTGCTG GGGCTGGAGC GAGAGGAAAA 
TTGTCCCCTC GGAAAGGCAA GAGTAAGACC TTGGACAATA GTGACTTGCA CCCAGGACCC 
AGTGCCGGTT CTCCTCCGCT CACCGTGCCA GCAATCCCAG TTCCAGCTAC TTCTGTCACT 
GCCGCCTCCA CGCAGCCCCT CGGGCCTGCA CCCCCTATCA CTCTGGAGCC ACCAGCTCCG 
GGGCTAAAAA GGGGCCGGGA GGGCGGCCGA GCGTCCACTC GTGACCGCAA GATGCTCAAG 
TTTATCAGCG GTATCTTCAC CAAGAGCACA GGAGGGCCTC CCGGTCCCGG GCCCCTTCCC 
GGACCCCAAG GCTTGTCTTC CAGCAGCGGG TCCAGGGAGC TGCTGGGCGC AGAGCTACGC 
GCCTCCCCTA AGGCTGTGGT CAATAGTCAG GAATGGACTT TGAGCCGATC AATTCCCGAA 
CTGCGCCTGG GTGTGCTGGG TGATGTCAGG AGTGGGAAGT CATCCCTCAT CCACAGATTC 
CTCACGGGTT CATACCAGGT GCTGGAGAAG CCTGAGAGTG AGCAGTACAA GAAGGAGATG 
CTGGTGGATG GGCAGACCCA TCTGGTGCTG ATCCGAGAGG AAGCAGGGGC ACCGGATGCC 
AAGTTCTCAG GCTGGGCAGA TGCTGTGATC TTCGTCTTCA GCCTGGAGGA TGAGAGCAGC 
TTCCAGGCTG TGAGCCATCT CCATGGGCAG CTGATCTCCC TCCGGGGGGA GGGACGAGGA 
GGTCTGGCCC TTGCCCTGGT TGGGACCCAA GACAGGATCA GCGCATCGTC CCCTCGAGTG 
GTGGGTGATG CTCGAGCCCG GGCATTGTGT ACCGACATGA AGCGCTGCAG CTACTATGAG 
ACCTGTGCAA CCTATGGGCT CAATGTGGAC CGGGTCTTCC AGGAGGTGGC CCAGAAGGTG 
GTGACCTTGC GCAAACAGCA GCAACTCCTG GCGGCTTGCA AGTCCCTGCC CAGCTCACCG 
AGCCATTCAG CTGCTTCTAC TCCTGTAGCT GGGCAGGCTA GTAATGGGGG CCACACTAGC 
GACTACTCTT CTTCCCTCCC ATCTTCACCC AATGTTGGAC ACCGGGAGCT CCGGGCTGAG 
GCAGCTGCGG TGGCTGGGTT GAGCACCCCA GGGTCCTTAC ATCGAGCAGC CAAGCGGCGG 
ACCAGCCTTT TTGCGAACCG TCGAGGCAGT GATTCTGAGA AACGGAGCTT AGACAGTCGG 
GGCGAGACAA CAGGGAGTGG GCGAGCCATC CCCATTAAAC AGAGTTTCCT GCTAAAGCGA 
AGTGGCAATT CCTTGAACAA AGAGTGGAAG AAGAAATACG TGACCTTGTC CAGCAATGGC 
TTTCTGCTCT ACCACCCTAG CATTAATGAT TACATCCACA GTACACACGG CAAAGAGATG 
GACTTGTTAC GAACAACAGT CAAAGTCCCA GGCAAGCGGC CCCCAAGGGC TATCTCTGCT 
TTCGGCCCCT CAGCCAGCAT CAATGGGTTG GTCAAGGATA TGAGCACTGT CCAGATGGGT 
GAAGGCCCTG AAGCCTCAAC TCCAATGCCA AGCCCCAGTC CCAGCCCCAG CTCCCTGCAG 
CTCCCAACAG ACCAAACATC CAAGCACCTG TTGAAGCCAG ACCGGAATTT GGCCCGTGCC 
CTTAGCACCG ACTGTACCCC ATCTGGAGAC CTGAGTCCCC TGAGTCGGGA ACCCCCACCT 
TCTCCCATGG TGAAGAAGCA GAGGAGGAAG AAATTGTCCA CACCATCTAA GACTGAAGGC 
TCAGCTGTGC AGGCTGAAGC CAAGCGCAAA ATGTGGAAAC TAAAATCCTT TGGTAGTTTA 
AGAAATATTT ATAAAGCAGA GGAAAACTTT GAGTTCCTGA TCGTGTCCAG CACTGGTCAG 
ACGTGGCACT TTGAGGCAGC CAGCTTCGAG GAGAGGGATG CCTGGGTTCA GGCCATTGAG 
AGTCAGATCC TGGCCAGTCT GCAATGCTGT GAGAGCAGCA AGGTCAAGCT GCGCACTGAC 
AGCCAAAGTG AAGCCGTGGC CATCCAGGCG ATCCGCAACG CAAAGGGAAA CTCGACCTGT 
GTGGACTGTG GAGCCCCCAA CCCCACGTGG GCCAGTTTGA ATCTGGGCGC ACTCATCTGC 
ATCGAATGTT CTGGCATCCA CCGGAACCTG GGCACGCACC TGTCTCGAGT GCGTTCGCTG 
GACTTGGATG ACTGGCCGCG GGAGCTGACC CTGGTGCTGA CGGCCATTGG CAACGACACG 
GCCAATCGCG TGTGGGAGAG CGACACTCGG GGCCGTGCCA AGCCCACGCG GGACTCTTCG 
AGGGAGGAGC GTGAGTCGTG GATTCGGGCC AAGTACGAAC AGCTGCTGTT CCTGGCGCCT 
CTGGGCACCA CGGAGGAGCC GCTGGGCCGC CAACTGTGGG CCGCTGTGGA GGCCCAGGAC 
GTGGCCGCCG TTCTGCTGCT TCTGGCCCAC GCGCGACATG GGCCGCTGGA CACCAGCGTG 
GAGGACCCTC AGCTCCGCTC GCCGCTGCAC TTGGCGGCCG AGCTCGCCCA CGTTGTTATT 
ACACAGCTGC TGTTGTGGTA TGGCGCAGAC GTGGCGGCCC GCGATGCGCA GGGCCGCACA 
GCATTGTTCT ACGCCCGCCA GGCTGGCAGC CAGCTATGTG CAGACATCCT CCTCCAGCAC 
GGCTGTCCGG GAGAGGGCGG CAGTACAGCC ACCACGCCCA GCGCGGCCAC CACCCCCAGC 
ATTACCGCCA CGCCCAGCCC GAGGCGCCGG AGCAGTGCTG CCAGCTTGGG TCGCGTGGAC 
ACCACGATCG CGCTGGTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agap2 ( XM_006241499.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006241561.1 Rattus norvegicus ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 (Agap2), transcript variant X1, mRNA.

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CloneID	ORa30279
Clone ID Related Accession (Same CDS sequence)	XM_006241499.3
Accession Version	XM_006241499.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3501bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006241499.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCGGG GCGCGGGCGC GCTTCAGCGC CGGACAACGA CCTACCTCAT CTCGCTGACC 
CTGGTCAAGC TCGAGTCGGT ACCTCCGCCG CCGCCTTCTC CGTCTGCAGC TGCGGTCGGC 
GCCCCTGGGG CCAGAGGCTC GGAGCCCCGA GATCCTGGCA GCCCCCGAGG CGCGGAGGAA 
CCCGGCAAGA AGCGGCACGA GCGTCTCTTC CACCGGCAGG ATGCGCTGTG GATCAGTACC 
AGCAGCGCGG GCGCCGGGGG CGCGGAGCCC CCCGCGCTGT CCCCGGCTCC GGCCAGTCCG 
GCTCGCCCGG TCTCCCCGGC TCCGGGCCGC CGACTGTCCC TCTGGGCCGC CCCTCCGGGA 
CCCCCGCTCT CCGGGGGGCT GAGCCCGGAC TCCAAGCCGG GGGGCGCCCC CTCTTCCTCT 
CGGCGCCCTC TACTCAGCAG CCCGAGCTGG GGGGGTCCGG AACCTGAAGG CCGGACGGGT 
GGCGGCGTCC CGGGCTCGTC CTCCCCGCAT CCTGGCACCG GTAGCCGAAG GCTCAAGGTG 
GCGCCTCCTC CGCCAGCTCC CAAGCCTTTC AAGACCGTGA CCACTAGTGG AGCCAAAGCC 
GGCGGGGGCA AGGGCGCGGG TAGCCGCCTG TCATGGCCCG AAAGCGAGGG CAAACCCAGG 
GTCAAGGGGT CAAAGAGCAC CGCCGGGACT GGAGCCTCTG CCGTTGCGGC CGGCGGGGGA 
GGGAGCGCTG CAGTCACGAC CTCTGGTGGG GTCGGTGCTG GGGCTGGAGC GAGAGGAAAA 
TTGTCCCCTC GGAAAGGCAA GAGTAAGACC TTGGACAATA GTGACTTGCA CCCAGGACCC 
AGTGCCGGTT CTCCTCCGCT CACCGTGCCA GCAATCCCAG TTCCAGCTAC TTCTGTCACT 
GCCGCCTCCA CGCAGCCCCT CGGGCCTGCA CCCCCTATCA CTCTGGAGCC ACCAGCTCCG 
GGGCTAAAAA GGGGCCGGGA GGGCGGCCGA GCGTCCACTC GTGACCGCAA GATGCTCAAG 
TTTATCAGCG GTATCTTCAC CAAGAGCACA GGAGGGCCTC CCGGTCCCGG GCCCCTTCCC 
GGACCCCAAG GCTTGTCTTC CAGCAGCGGG TCCAGGGAGC TGCTGGGCGC AGAGCTACGC 
GCCTCCCCTA AGGCTGTGGT CAATAGTCAG GAATGGACTT TGAGCCGATC AATTCCCGAA 
CTGCGCCTGG GTGTGCTGGG TGATGTCAGG AGTGGGAAGT CATCCCTCAT CCACAGATTC 
CTCACGGGTT CATACCAGGT GCTGGAGAAG CCTGAGAGTG AGCAGTACAA GAAGGAGATG 
CTGGTGGATG GGCAGACCCA TCTGGTGCTG ATCCGAGAGG AAGCAGGGGC ACCGGATGCC 
AAGTTCTCAG GCTGGGCAGA TGCTGTGATC TTCGTCTTCA GCCTGGAGGA TGAGAGCAGC 
TTCCAGGCTG TGAGCCATCT CCATGGGCAG CTGATCTCCC TCCGGGGGGA GGGACGAGGA 
GGTCTGGCCC TTGCCCTGGT TGGGACCCAA GACAGGATCA GCGCATCGTC CCCTCGAGTG 
GTGGGTGATG CTCGAGCCCG GGCATTGTGT ACCGACATGA AGCGCTGCAG CTACTATGAG 
ACCTGTGCAA CCTATGGGCT CAATGTGGAC CGGGTCTTCC AGGAGGTGGC CCAGAAGGTG 
GTGACCTTGC GCAAACAGCA GCAACTCCTG GCGGCTTGCA AGTCCCTGCC CAGCTCACCG 
AGCCATTCAG CTGCTTCTAC TCCTGTAGCT GGGCAGGCTA GTAATGGGGG CCACACTAGC 
GACTACTCTT CTTCCCTCCC ATCTTCACCC AATGTTGGAC ACCGGGAGCT CCGGGCTGAG 
GCAGCTGCGG TGGCTGGGTT GAGCACCCCA GGGTCCTTAC ATCGAGCAGC CAAGCGGCGG 
ACCAGCCTTT TTGCGAACCG TCGAGGCAGT GATTCTGAGA AACGGAGCTT AGACAGTCGG 
GGCGAGACAA CAGGGAGTGG GCGAGCCATC CCCATTAAAC AGAGTTTCCT GCTAAAGCGA 
AGTGGCAATT CCTTGAACAA AGAGTGGAAG AAGAAATACG TGACCTTGTC CAGCAATGGC 
TTTCTGCTCT ACCACCCTAG CATTAATGAT TACATCCACA GTACACACGG CAAAGAGATG 
GACTTGTTAC GAACAACAGT CAAAGTCCCA GGCAAGCGGC CCCCAAGGGC TATCTCTGCT 
TTCGGCCCCT CAGCCAGCAT CAATGGGTTG GTCAAGGATA TGAGCACTGT CCAGATGGGT 
GAAGGCCCTG AAGCCTCAAC TCCAATGCCA AGCCCCAGTC CCAGCCCCAG CTCCCTGCAG 
CTCCCAACAG ACCAAACATC CAAGCACCTG TTGAAGCCAG ACCGGAATTT GGCCCGTGCC 
CTTAGCACCG ACTGTACCCC ATCTGGAGAC CTGAGTCCCC TGAGTCGGGA ACCCCCACCT 
TCTCCCATGG TGAAGAAGCA GAGGAGGAAG AAATTGTCCA CACCATCTAA GACTGAAGGC 
TCAGCTGTGC AGGCTGAAGA GGAAAACTTT GAGTTCCTGA TCGTGTCCAG CACTGGTCAG 
ACGTGGCACT TTGAGGCAGC CAGCTTCGAG GAGAGGGATG CCTGGGTTCA GGCCATTGAG 
AGTCAGATCC TGGCCAGTCT GCAATGCTGT GAGAGCAGCA AGGTCAAGCT GCGCACTGAC 
AGCCAAAGTG AAGCCGTGGC CATCCAGGCG ATCCGCAACG CAAAGGGAAA CTCGACCTGT 
GTGGACTGTG GAGCCCCCAA CCCCACGTGG GCCAGTTTGA ATCTGGGCGC ACTCATCTGC 
ATCGAATGTT CTGGCATCCA CCGGAACCTG GGCACGCACC TGTCTCGAGT GCGTTCGCTG 
GACTTGGATG ACTGGCCGCG GGAGCTGACC CTGGTGCTGA CGGCCATTGG CAACGACACG 
GCCAATCGCG TGTGGGAGAG CGACACTCGG GGCCGTGCCA AGCCCACGCG GGACTCTTCG 
AGGGAGGAGC GTGAGTCGTG GATTCGGGCC AAGTACGAAC AGCTGCTGTT CCTGGCGCCT 
CTGGGCACCA CGGAGGAGCC GCTGGGCCGC CAACTGTGGG CCGCTGTGGA GGCCCAGGAC 
GTGGCCGCCG TTCTGCTGCT TCTGGCCCAC GCGCGACATG GGCCGCTGGA CACCAGCGTG 
GAGGACCCTC AGCTCCGCTC GCCGCTGCAC TTGGCGGCCG AGCTCGCCCA CGTTGTTATT 
ACACAGCTGC TGTTGTGGTA TGGCGCAGAC GTGGCGGCCC GCGATGCGCA GGGCCGCACA 
GCATTGTTCT ACGCCCGCCA GGCTGGCAGC CAGCTATGTG CAGACATCCT CCTCCAGCAC 
GGCTGTCCGG GAGAGGGCGG CAGTACAGCC ACCACGCCCA GCGCGGCCAC CACCCCCAGC 
ATTACCGCCA CGCCCAGCCC GAGGCGCCGG AGCAGTGCTG CCAGCTTGGG TCGCGTGGAC 
ACCACGATCG CGCTGGTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006241561.1
CDS	759..4259
Translation	MSRGAGALQRRTTTYLISLTLVKLESVPPPPPSPSAAAVGAPGARGSEPRDPGSPRGAEEPGKKRHERLFHRQDALWISTSSAGAGGAEPPALSPAPASPARPVSPAPGRRLSLWAAPPGPPLSGGLSPDSKPGGAPSSSRRPLLSSPSWGGPEPEGRTGGGVPGSSSPHPGTGSRRLKVAPPPPAPKPFKTVTTSGAKAGGGKGAGSRLSWPESEGKPRVKGSKSTAGTGASAVAAGGGGSAAVTTSGGVGAGAGARGKLSPRKGKSKTLDNSDLHPGPSAGSPPLTVPAIPVPATSVTAASTQPLGPAPPITLEPPAPGLKRGREGGRASTRDRKMLKFISGIFTKSTGGPPGPGPLPGPQGLSSSSGSRELLGAELRASPKAVVNSQEWTLSRSIPELRLGVLGDVRSGKSSLIHRFLTGSYQVLEKPESEQYKKEMLVDGQTHLVLIREEAGAPDAKFSGWADAVIFVFSLEDESSFQAVSHLHGQLISLRGEGRGGLALALVGTQDRISASSPRVVGDARARALCTDMKRCSYYETCATYGLNVDRVFQEVAQKVVTLRKQQQLLAACKSLPSSPSHSAASTPVAGQASNGGHTSDYSSSLPSSPNVGHRELRAEAAAVAGLSTPGSLHRAAKRRTSLFANRRGSDSEKRSLDSRGETTGSGRAIPIKQSFLLKRSGNSLNKEWKKKYVTLSSNGFLLYHPSINDYIHSTHGKEMDLLRTTVKVPGKRPPRAISAFGPSASINGLVKDMSTVQMGEGPEASTPMPSPSPSPSSLQLPTDQTSKHLLKPDRNLARALSTDCTPSGDLSPLSREPPPSPMVKKQRRKKLSTPSKTEGSAVQAEEENFEFLIVSSTGQTWHFEAASFEERDAWVQAIESQILASLQCCESSKVKLRTDSQSEAVAIQAIRNAKGNSTCVDCGAPNPTWASLNLGALICIECSGIHRNLGTHLSRVRSLDLDDWPRELTLVLTAIGNDTANRVWESDTRGRAKPTRDSSREERESWIRAKYEQLLFLAPLGTTEEPLGRQLWAAVEAQDVAAVLLLLAHARHGPLDTSVEDPQLRSPLHLAAELAHVVITQLLLWYGADVAARDAQGRTALFYARQAGSQLCADILLQHGCPGEGGSTATTPSAATTPSITATPSPRRRSSAASLGRVDTTIALV

Target ORF information:

RefSeq Version	XM_006241499.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 (Agap2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006241499.3

ORF Insert Sequence:

ATGAGCCGGG GCGCGGGCGC GCTTCAGCGC CGGACAACGA CCTACCTCAT CTCGCTGACC 
CTGGTCAAGC TCGAGTCGGT ACCTCCGCCG CCGCCTTCTC CGTCTGCAGC TGCGGTCGGC 
GCCCCTGGGG CCAGAGGCTC GGAGCCCCGA GATCCTGGCA GCCCCCGAGG CGCGGAGGAA 
CCCGGCAAGA AGCGGCACGA GCGTCTCTTC CACCGGCAGG ATGCGCTGTG GATCAGTACC 
AGCAGCGCGG GCGCCGGGGG CGCGGAGCCC CCCGCGCTGT CCCCGGCTCC GGCCAGTCCG 
GCTCGCCCGG TCTCCCCGGC TCCGGGCCGC CGACTGTCCC TCTGGGCCGC CCCTCCGGGA 
CCCCCGCTCT CCGGGGGGCT GAGCCCGGAC TCCAAGCCGG GGGGCGCCCC CTCTTCCTCT 
CGGCGCCCTC TACTCAGCAG CCCGAGCTGG GGGGGTCCGG AACCTGAAGG CCGGACGGGT 
GGCGGCGTCC CGGGCTCGTC CTCCCCGCAT CCTGGCACCG GTAGCCGAAG GCTCAAGGTG 
GCGCCTCCTC CGCCAGCTCC CAAGCCTTTC AAGACCGTGA CCACTAGTGG AGCCAAAGCC 
GGCGGGGGCA AGGGCGCGGG TAGCCGCCTG TCATGGCCCG AAAGCGAGGG CAAACCCAGG 
GTCAAGGGGT CAAAGAGCAC CGCCGGGACT GGAGCCTCTG CCGTTGCGGC CGGCGGGGGA 
GGGAGCGCTG CAGTCACGAC CTCTGGTGGG GTCGGTGCTG GGGCTGGAGC GAGAGGAAAA 
TTGTCCCCTC GGAAAGGCAA GAGTAAGACC TTGGACAATA GTGACTTGCA CCCAGGACCC 
AGTGCCGGTT CTCCTCCGCT CACCGTGCCA GCAATCCCAG TTCCAGCTAC TTCTGTCACT 
GCCGCCTCCA CGCAGCCCCT CGGGCCTGCA CCCCCTATCA CTCTGGAGCC ACCAGCTCCG 
GGGCTAAAAA GGGGCCGGGA GGGCGGCCGA GCGTCCACTC GTGACCGCAA GATGCTCAAG 
TTTATCAGCG GTATCTTCAC CAAGAGCACA GGAGGGCCTC CCGGTCCCGG GCCCCTTCCC 
GGACCCCAAG GCTTGTCTTC CAGCAGCGGG TCCAGGGAGC TGCTGGGCGC AGAGCTACGC 
GCCTCCCCTA AGGCTGTGGT CAATAGTCAG GAATGGACTT TGAGCCGATC AATTCCCGAA 
CTGCGCCTGG GTGTGCTGGG TGATGTCAGG AGTGGGAAGT CATCCCTCAT CCACAGATTC 
CTCACGGGTT CATACCAGGT GCTGGAGAAG CCTGAGAGTG AGCAGTACAA GAAGGAGATG 
CTGGTGGATG GGCAGACCCA TCTGGTGCTG ATCCGAGAGG AAGCAGGGGC ACCGGATGCC 
AAGTTCTCAG GCTGGGCAGA TGCTGTGATC TTCGTCTTCA GCCTGGAGGA TGAGAGCAGC 
TTCCAGGCTG TGAGCCATCT CCATGGGCAG CTGATCTCCC TCCGGGGGGA GGGACGAGGA 
GGTCTGGCCC TTGCCCTGGT TGGGACCCAA GACAGGATCA GCGCATCGTC CCCTCGAGTG 
GTGGGTGATG CTCGAGCCCG GGCATTGTGT ACCGACATGA AGCGCTGCAG CTACTATGAG 
ACCTGTGCAA CCTATGGGCT CAATGTGGAC CGGGTCTTCC AGGAGGTGGC CCAGAAGGTG 
GTGACCTTGC GCAAACAGCA GCAACTCCTG GCGGCTTGCA AGTCCCTGCC CAGCTCACCG 
AGCCATTCAG CTGCTTCTAC TCCTGTAGCT GGGCAGGCTA GTAATGGGGG CCACACTAGC 
GACTACTCTT CTTCCCTCCC ATCTTCACCC AATGTTGGAC ACCGGGAGCT CCGGGCTGAG 
GCAGCTGCGG TGGCTGGGTT GAGCACCCCA GGGTCCTTAC ATCGAGCAGC CAAGCGGCGG 
ACCAGCCTTT TTGCGAACCG TCGAGGCAGT GATTCTGAGA AACGGAGCTT AGACAGTCGG 
GGCGAGACAA CAGGGAGTGG GCGAGCCATC CCCATTAAAC AGAGTTTCCT GCTAAAGCGA 
AGTGGCAATT CCTTGAACAA AGAGTGGAAG AAGAAATACG TGACCTTGTC CAGCAATGGC 
TTTCTGCTCT ACCACCCTAG CATTAATGAT TACATCCACA GTACACACGG CAAAGAGATG 
GACTTGTTAC GAACAACAGT CAAAGTCCCA GGCAAGCGGC CCCCAAGGGC TATCTCTGCT 
TTCGGCCCCT CAGCCAGCAT CAATGGGTTG GTCAAGGATA TGAGCACTGT CCAGATGGGT 
GAAGGCCCTG AAGCCTCAAC TCCAATGCCA AGCCCCAGTC CCAGCCCCAG CTCCCTGCAG 
CTCCCAACAG ACCAAACATC CAAGCACCTG TTGAAGCCAG ACCGGAATTT GGCCCGTGCC 
CTTAGCACCG ACTGTACCCC ATCTGGAGAC CTGAGTCCCC TGAGTCGGGA ACCCCCACCT 
TCTCCCATGG TGAAGAAGCA GAGGAGGAAG AAATTGTCCA CACCATCTAA GACTGAAGGC 
TCAGCTGTGC AGGCTGAAGA GGAAAACTTT GAGTTCCTGA TCGTGTCCAG CACTGGTCAG 
ACGTGGCACT TTGAGGCAGC CAGCTTCGAG GAGAGGGATG CCTGGGTTCA GGCCATTGAG 
AGTCAGATCC TGGCCAGTCT GCAATGCTGT GAGAGCAGCA AGGTCAAGCT GCGCACTGAC 
AGCCAAAGTG AAGCCGTGGC CATCCAGGCG ATCCGCAACG CAAAGGGAAA CTCGACCTGT 
GTGGACTGTG GAGCCCCCAA CCCCACGTGG GCCAGTTTGA ATCTGGGCGC ACTCATCTGC 
ATCGAATGTT CTGGCATCCA CCGGAACCTG GGCACGCACC TGTCTCGAGT GCGTTCGCTG 
GACTTGGATG ACTGGCCGCG GGAGCTGACC CTGGTGCTGA CGGCCATTGG CAACGACACG 
GCCAATCGCG TGTGGGAGAG CGACACTCGG GGCCGTGCCA AGCCCACGCG GGACTCTTCG 
AGGGAGGAGC GTGAGTCGTG GATTCGGGCC AAGTACGAAC AGCTGCTGTT CCTGGCGCCT 
CTGGGCACCA CGGAGGAGCC GCTGGGCCGC CAACTGTGGG CCGCTGTGGA GGCCCAGGAC 
GTGGCCGCCG TTCTGCTGCT TCTGGCCCAC GCGCGACATG GGCCGCTGGA CACCAGCGTG 
GAGGACCCTC AGCTCCGCTC GCCGCTGCAC TTGGCGGCCG AGCTCGCCCA CGTTGTTATT 
ACACAGCTGC TGTTGTGGTA TGGCGCAGAC GTGGCGGCCC GCGATGCGCA GGGCCGCACA 
GCATTGTTCT ACGCCCGCCA GGCTGGCAGC CAGCTATGTG CAGACATCCT CCTCCAGCAC 
GGCTGTCCGG GAGAGGGCGG CAGTACAGCC ACCACGCCCA GCGCGGCCAC CACCCCCAGC 
ATTACCGCCA CGCCCAGCCC GAGGCGCCGG AGCAGTGCTG CCAGCTTGGG TCGCGTGGAC 
ACCACGATCG CGCTGGTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Reduced expression of E-cadherin and p120-catenin and elevated expression of PLC-γ1 and PIKE are associated with aggressiveness of oral squamous cell carcinoma.
International journal of clinical and experimental pathology8(8)9042-51(2015)
Jiang Y,Liao L,Shrestha C,Ji S,Chen Y,Peng J,Wang L,Liao E,Xie Z

PIKE is essential for oligodendroglia development and CNS myelination.
Proceedings of the National Academy of Sciences of the United States of America111(5)1993-8(2014 Feb)
Chan CB,Liu X,Zhao L,Liu G,Lee CW,Feng Y,Ye K

RACK1 promotes neurite outgrowth by scaffolding AGAP2 to FAK.
Cellular signalling26(1)9-18(2014 Jan)
Dwane S,Durack E,O'Connor R,Kiely PA

Essential role of PIKE GTPases in neuronal protection against excitotoxic insults.
Advances in biological regulation52(1)66-76(2012 Jan)
Chan CB,Chen Y,Liu X,Papale L,Escayg A,Mei L,Ye K

PIKE-mediated PI3-kinase activity is required for AMPA receptor surface expression.
The EMBO journal30(20)4274-86(2011 Aug)
Chan CB,Chen Y,Liu X,Tang X,Lee CW,Mei L,Ye K

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Agap2 cDNA ORF clone, Rattus norvegicus(Norway rat)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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