Ncs1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ncs1
Entrez Gene ID	65153
Full Name	neuronal calcium sensor 1
Synonyms	Freq
General protein information	Preferred Names neuronal calcium sensor 1 Names neuronal calcium sensor 1 NCS-1 frequenin-like protein frequenin-like ubiquitous protein
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3p12
Summary	mediates increased pohosphinositide turnover and Ca2+ signaling; plays a role in positive regulation of exocytosis [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_024366.1	NP_077342.1	neuronal calcium sensor 1

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP013126	XP_003437186.1
Neurospora crassa	NCU04379	XP_957456.1
Mus musculus (house mouse)	Ncs1	NP_062655.1
Gallus gallus (chicken)	NCS1	NP_990708.1
Drosophila melanogaster (fruit fly)	Frq1	NP_573271.1
Kluyveromyces lactis	KLLA0E02399g	XP_454056.1
Schizosaccharomyces pombe (fission yeast)	ncs1	NP_592879.1
Eremothecium gossypii	AGOS_ACL163W	NP_983241.1
Danio rerio (zebrafish)	ncs1b	NP_001018350.1
Homo sapiens (human)	NCS1	NP_055101.2
Bos taurus (cattle)	NCS1	XP_005213538.1
Rattus norvegicus (Norway rat)	Ncs1	NP_077342.1
Xenopus tropicalis (tropical clawed frog)	ncs1	NP_988973.1
Canis lupus familiaris (dog)	NCS1	XP_005625293.1
Drosophila melanogaster (fruit fly)	Frq2	NP_001259675.1
Caenorhabditis elegans (roundworm)	ncs-1	NP_508186.1
Saccharomyces cerevisiae (baker's yeast)	FRQ1	NP_010661.3
Magnaporthe oryzae (rice blast fungus)	MGG_01550	XP_003714524.1

Gene Ontology
Bibliography

Related articles in PubMed

Identification of critical amino acid residues and functional conservation of the Neurospora crassa and Rattus norvegicus orthologues of neuronal calcium sensor-1.
Gohain D, Deka R, Tamuli R
Genetica144(6)665-674(2016 Dec)

Neuronal Calcium Sensor-1 Binds the D2 Dopamine Receptor and G-protein-coupled Receptor Kinase 1 (GRK1) Peptides Using Different Modes of Interactions.
Pandalaneni S, Karuppiah V, Saleem M, Haynes LP, Burgoyne RD, Mayans O, Derrick JP, Lian LY
The Journal of biological chemistry290(30)18744-56(2015 Jul)

Evaluation of NCS-1, DARPP-32, and neurotrophins in hippocampus and prefrontal cortex in rats submitted to sepsis.
Comim CM, Silva NC, Mina F, Dominguini D, Scaini G, Morais MO, Rosa DV, Magno LA, Streck EL, Romano-Silva MA, Quevedo J, Dal-Pizzol F
Synapse (New York, N.Y.)68(10)474-9(2014 Oct)

Differential effects of swimming training on neuronal calcium sensor-1 expression in rat hippocampus/cortex and in object recognition memory tasks.
Drumond LE, Mourão FA, Leite HR, Abreu RV, Reis HJ, Moraes MF, Pereira GS, Massensini AR
Brain research bulletin88(4)385-91(2012 Jul)

Characterization of low-energy excited states in the native state ensemble of non-myristoylated and myristoylated neuronal calcium sensor-1.
Chandra K, Sharma Y, Chary KV
Biochimica et biophysica acta1814(2)334-44(2011 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ncs1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ncs1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa06950	NM_024366.1 Latest version!	Rattus norvegicus neuronal calcium sensor 1 (Ncs1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ncs1 ( NM_024366.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_077342.1 Rattus norvegicus neuronal calcium sensor 1 (Ncs1), mRNA.

Price & Availability↑

CloneID	ORa06950
Clone ID Related Accession (Same CDS sequence)	NM_024366.1
Accession Version	NM_024366.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 573bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577203200000
Organism	Rattus norvegicus(Norway rat)
Product	neuronal calcium sensor 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X82188.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X82188.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689602 [ECO:0000348] ##Evidence-Data-END##

ATGGGGAAAT CCAACAGCAA GTTGAAGCCT GAAGTTGTGG AGGAGCTGAC CAGAAAAACC 
TACTTCACTG AAAAGGAAGT ACAGCAGTGG TACAAGGGTT TCATTAAGGA CTGCCCCAGT 
GGGCAGCTGG ACGCCGCTGG CTTCCAGAAG ATCTACAAAC AGTTCTTCCC ATTTGGAGAC 
CCCACCAAGT TCGCCACGTT TGTTTTCAAC GTCTTCGACG AGAACAAGGA TGGCAGGATC 
GAGTTCTCCG AATTCATCCA GGCTCTATCG GTGACCTCAA GGGGGACCCT GGATGAGAAG 
TTGAGGTGGG CCTTCAAGCT TTATGACTTG GATAACGACG GCTACATCAC CAGAAACGAG 
ATGCTGGACA TAGTGGATGC CATTTACCAG ATGGTGGGGA ACACCGTGGA GCTCCCAGAA 
GAAGAGAACA CACCAGAGAA GAGGGTGGAC CGGATCTTCG CCATGATGGA TAAGAACGCT 
GATGGGAAGC TAACTCTTCA GGAGTTCCAG GAAGGCTCCA AGGCCGACCC CTCCATTGTG 
CAGGCGCTGT CCCTCTACGA CGGGCTGGTA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_077342.1
CDS	61..633
Translation	MGKSNSKLKPEVVEELTRKTYFTEKEVQQWYKGFIKDCPSGQLDAAGFQKIYKQFFPFGDPTKFATFVFNVFDENKDGRIEFSEFIQALSVTSRGTLDEKLRWAFKLYDLDNDGYITRNEMLDIVDAIYQMVGNTVELPEEENTPEKRVDRIFAMMDKNADGKLTLQEFQEGSKADPSIVQALSLYDGLV

Target ORF information:

RefSeq Version	NM_024366.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus neuronal calcium sensor 1 (Ncs1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024366.1

ORF Insert Sequence:

ATGGGGAAAT CCAACAGCAA GTTGAAGCCT GAAGTTGTGG AGGAGCTGAC CAGAAAAACC 
TACTTCACTG AAAAGGAAGT ACAGCAGTGG TACAAGGGTT TCATTAAGGA CTGCCCCAGT 
GGGCAGCTGG ACGCCGCTGG CTTCCAGAAG ATCTACAAAC AGTTCTTCCC ATTTGGAGAC 
CCCACCAAGT TCGCCACGTT TGTTTTCAAC GTCTTCGACG AGAACAAGGA TGGCAGGATC 
GAGTTCTCCG AATTCATCCA GGCTCTATCG GTGACCTCAA GGGGGACCCT GGATGAGAAG 
TTGAGGTGGG CCTTCAAGCT TTATGACTTG GATAACGACG GCTACATCAC CAGAAACGAG 
ATGCTGGACA TAGTGGATGC CATTTACCAG ATGGTGGGGA ACACCGTGGA GCTCCCAGAA 
GAAGAGAACA CACCAGAGAA GAGGGTGGAC CGGATCTTCG CCATGATGGA TAAGAACGCT 
GATGGGAAGC TAACTCTTCA GGAGTTCCAG GAAGGCTCCA AGGCCGACCC CTCCATTGTG 
CAGGCGCTGT CCCTCTACGA CGGGCTGGTA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Identification of critical amino acid residues and functional conservation of the Neurospora crassa and Rattus norvegicus orthologues of neuronal calcium sensor-1.
Genetica144(6)665-674(2016 Dec)
Gohain D,Deka R,Tamuli R

Neuronal Calcium Sensor-1 Binds the D2 Dopamine Receptor and G-protein-coupled Receptor Kinase 1 (GRK1) Peptides Using Different Modes of Interactions.
The Journal of biological chemistry290(30)18744-56(2015 Jul)
Pandalaneni S,Karuppiah V,Saleem M,Haynes LP,Burgoyne RD,Mayans O,Derrick JP,Lian LY

Evaluation of NCS-1, DARPP-32, and neurotrophins in hippocampus and prefrontal cortex in rats submitted to sepsis.
Synapse (New York, N.Y.)68(10)474-9(2014 Oct)
Comim CM,Silva NC,Mina F,Dominguini D,Scaini G,Morais MO,Rosa DV,Magno LA,Streck EL,Romano-Silva MA,Quevedo J,Dal-Pizzol F

Differential effects of swimming training on neuronal calcium sensor-1 expression in rat hippocampus/cortex and in object recognition memory tasks.
Brain research bulletin88(4)385-91(2012 Jul)
Drumond LE,Mourão FA,Leite HR,Abreu RV,Reis HJ,Moraes MF,Pereira GS,Massensini AR

Characterization of low-energy excited states in the native state ensemble of non-myristoylated and myristoylated neuronal calcium sensor-1.
Biochimica et biophysica acta1814(2)334-44(2011 Feb)
Chandra K,Sharma Y,Chary KV