B4galnt1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	B4galnt1
Entrez Gene ID	64828
Full Name	beta-1,4-N-acetyl-galactosaminyl transferase 1
Synonyms	GalNAc-T,Galgt1
General protein information	Preferred Names beta-1,4-N-acetyl-galactosaminyl transferase 1 Names beta-1,4 N-acetylgalactosaminyltransferase 1 (N-acetylneuraminyl)-galactosylglucosylceramide GM2/GD2 synthase UDP-N-acetyl-alpha-D-galactosamine:(N-acetylneuraminyl)-galactosylglucosylceramide-beta-1, 4-N-acetylgalactosaminyltransferase beta-4N-acetylgalactosaminyltransferase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q22
Summary	synthesizes both asialoglycosphingolipid GM2 and glycosphingolipid GM2 [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_022860.2	NP_074051.1	beta-1,4 N-acetylgalactosaminyltransferase 1 precursor
XM_006241497.3	XP_006241559.1	beta-1,4 N-acetylgalactosaminyltransferase 1 isoform X2
XM_008765420.2	XP_008763642.1	beta-1,4 N-acetylgalactosaminyltransferase 1 isoform X1
XM_017595100.1	XP_017450589.1	beta-1,4 N-acetylgalactosaminyltransferase 1 isoform X3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	B4GALNT1	XP_509169.2
Macaca mulatta (Rhesus monkey)	B4GALNT1	XP_001116263.2
Rattus norvegicus (Norway rat)	B4galnt1	NP_074051.1
Bos taurus (cattle)	B4GALNT1	NP_001017943.1
Mus musculus (house mouse)	B4galnt1	NP_082015.2
Danio rerio (zebrafish)	LOC793635	XP_001333429.1
Xenopus tropicalis (tropical clawed frog)	b4galnt1	NP_001120168.1
Homo sapiens (human)	B4GALNT1	NP_001469.1
Canis lupus familiaris (dog)	B4GALNT1	XP_538250.3

Gene Ontology
Bibliography

Related articles in PubMed

Male germ cells require polyenoic sphingolipids with complex glycosylation for completion of meiosis: a link to ceramide synthase-3.
Rabionet M, van der Spoel AC, Chuang CC, von Tümpling-Radosta B, Litjens M, Bouwmeester D, Hellbusch CC, Körner C, Wiegandt H, Gorgas K, Platt FM, Gröne HJ, Sandhoff R
The Journal of biological chemistry283(19)13357-69(2008 May)

A genetic model of substrate deprivation therapy for a glycosphingolipid storage disorder.
Liu Y, Wada R, Kawai H, Sango K, Deng C, Tai T, McDonald MP, Araujo K, Crawley JN, Bierfreund U, Sandhoff K, Suzuki K, Proia RL
The Journal of clinical investigation103(4)497-505(1999 Feb)

Mice with disrupted GM2/GD2 synthase gene lack complex gangliosides but exhibit only subtle defects in their nervous system.
Takamiya K, Yamamoto A, Furukawa K, Yamashiro S, Shin M, Okada M, Fukumoto S, Haraguchi M, Takeda N, Fujimura K, Sakae M, Kishikawa M, Shiku H, Furukawa K, Aizawa S
Proceedings of the National Academy of Sciences of the United States of America93(20)10662-7(1996 Oct)

beta 1-4N-acetylgalactosaminyltransferase can synthesize both asialoglycosphingolipid GM2 and glycosphingolipid GM2 in vitro and in vivo: isolation and characterization of a beta 1-4N-acetylgalactosaminyltransferase cDNA clone from rat ascites hepatoma cell line AH7974F.
Hidari JK, Ichikawa S, Furukawa K, Yamasaki M, Hirabayashi Y
The Biochemical journal303 ( Pt 3)957-65(1994 Nov)

Expression cloning of beta 1,4 N-acetylgalactosaminyltransferase cDNAs that determine the expression of GM2 and GD2 gangliosides.
Nagata Y, Yamashiro S, Yodoi J, Lloyd KO, Shiku H, Furukawa K
The Journal of biological chemistry267(17)12082-9(1992 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following B4galnt1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the B4galnt1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa06382	NM_022860.2 Latest version!	Rattus norvegicus beta-1,4-N-acetyl-galactosaminyl transferase 1 (B4galnt1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ORa06382	XM_017595100.1 Latest version!	Rattus norvegicus beta-1,4-N-acetyl-galactosaminyl transferase 1 (B4galnt1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ORa30277	XM_008765420.2 Latest version!	Rattus norvegicus beta-1,4-N-acetyl-galactosaminyl transferase 1 (B4galnt1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ORa30278	XM_006241497.3 Latest version!	Rattus norvegicus beta-1,4-N-acetyl-galactosaminyl transferase 1 (B4galnt1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

B4galnt1 ( NM_022860.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_074051.1 Rattus norvegicus beta-1,4-N-acetyl-galactosaminyl transferase 1 (B4galnt1), mRNA.

Price & Availability↑

CloneID	ORa06382
Clone ID Related Accession (Same CDS sequence)	NM_022860.2 , XM_017595100.1
Accession Version	NM_022860.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577116800000
Organism	Rattus norvegicus(Norway rat)
Product	beta-1,4 N-acetylgalactosaminyltransferase 1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC081799.1. On Sep 16, 2004 this sequence version replaced NM_022860.1. ##Evidence-Data-START## Transcript exon combination :: BC081799.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGCGGCTGG ACCGCCGGGC CCTCTATGCG CTAGTTCTGC TGCTTGCCTG CGCCTCGCTG 
GGTCTCCTGT ACGCCAGCAC CCGAGACGCG CCAGGTCTCC CGAACCCTCT GGCATTGTGG 
TCACCCCCAC AAGGTCCCCC GAGGCTCGAT CTGCTAGACC TTGCCACTGA GCCTCGCTAC 
GCACACATCC CAGTCAGGAT CAAGGAGCAA GTGGTGGGGC TGCTGGCTCA GAACAATTGC 
AGTTGTGAGT CCAGCGGAGG ACGCTTTGCC TTGCCGTTCC TGAGGCAGGT CCGGGCGATT 
GACTTCACTA AAGCCTTTGA CGCCGAGGAG CTGAGGGCTG TTTCTATCTC CAGAGAGCAG 
GAATACCAGG CCTTCCTTGC AAGGAGCCGG TCCCTGGCTG ACCAGCTGCT GATAGCCCCT 
GCCAACTCCC CCTTACAGTA TCCCCTGCAG GGTGTGGAGG TTCAGCCCCT CAGGAGCATC 
CTGGTGCCAG GGCTAAGTCT GCAGGAAGCT TCTGTTCAGG AAATATATCA GGTGAACCTG 
ATTGCTTCCC TTGGCACCTG GGATGTGGCA GGGGAAGTAA CAGGGGTGAC TCTCACTGGA 
GAGGGGCAGT CGGACCTCAC CCTTGCCAGC CCAATTCTGG ATAAACTCAA CCGACAGCTG 
CAACTGGTGA CTTACAGCAG CCGGAGCTAC CAAGCCAACA CAGCAGACAC AGTCCGGTTC 
TCCACCAAGG GACATGAAGT GGCCTTCACC ATCCTCATAA GACATCCTCC CAACCCCCGG 
CTGTACCCAC CATCATCCCT ACCCCAAGGA GCCCAGTACA ACATCAGTGC TCTGGTTACC 
GTTGCCACCA AGACCTTTCT TCGTTATGAT CGGCTACGGG CACTCATTGC CAGCATCAGA 
CGCTTTTACC CTACGGTCAC CATAGTAATC GCTGACGACA GCGACAAACC GGAGCGAATT 
AGCGACCCCC ATGTGGAGCA CTATTTCATG CCCTTCGGCA AGGGTTGGTT TGCAGGTCGG 
AACCTGGCGG TGTCCCAAGT AACCACCAAA TACGTGCTGT GGGTGGACGA CGACTTTGTC 
TTCACGGCGC GCACGCGGCT GGAGAAGCTT GTGGATGTCC TGGAGAGGAC GCCCCTGGAC 
TTGGTTGGGG GCGCGGTGCG GGAGATCTCG GGCTACGCTA CCACCTACCG ACAGCTGCTA 
AGTGTGGAGC CGGGCGCCCC AGGCTTTGGG AACTGCCTCC GGCAAAAGCA GGGCTTCCAC 
CACGAGCTCG CTGGCTTTCC AAACTGCGTG GTCACCGACG GCGTAGTCAA CTTCTTCCTG 
GCGCGCACAG ATAAAGTGCG CCAGGTGGGC TTTGACCCAC GCCTCAACCG GGTGGCTCAT 
CTGGAATTCT TCCTGGATGG TCTTGGTTCC CTTCGAGTTG GCTCCTGCTC TGATGTTGTT 
GTGGATCATG CGTCAAAGGT GAAGCTGCCT TGGACATCAA AGGATCCAGG GGCTGAACTT 
TATGCCCGTT ACCGTTACCC GGGATCACTG GACCAAAGTC AGGTGGCCAA ACATCGACTG 
CTCTTCTTCA AACACCGGCT ACAGTGCATG ACCGCCGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_074051.1
CDS	142..1743
Translation	MRLDRRALYALVLLLACASLGLLYASTRDAPGLPNPLALWSPPQGPPRLDLLDLATEPRYAHIPVRIKEQVVGLLAQNNCSCESSGGRFALPFLRQVRAIDFTKAFDAEELRAVSISREQEYQAFLARSRSLADQLLIAPANSPLQYPLQGVEVQPLRSILVPGLSLQEASVQEIYQVNLIASLGTWDVAGEVTGVTLTGEGQSDLTLASPILDKLNRQLQLVTYSSRSYQANTADTVRFSTKGHEVAFTILIRHPPNPRLYPPSSLPQGAQYNISALVTVATKTFLRYDRLRALIASIRRFYPTVTIVIADDSDKPERISDPHVEHYFMPFGKGWFAGRNLAVSQVTTKYVLWVDDDFVFTARTRLEKLVDVLERTPLDLVGGAVREISGYATTYRQLLSVEPGAPGFGNCLRQKQGFHHELAGFPNCVVTDGVVNFFLARTDKVRQVGFDPRLNRVAHLEFFLDGLGSLRVGSCSDVVVDHASKVKLPWTSKDPGAELYARYRYPGSLDQSQVAKHRLLFFKHRLQCMTAE

Target ORF information:

RefSeq Version	NM_022860.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus beta-1,4-N-acetyl-galactosaminyl transferase 1 (B4galnt1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022860.2

ORF Insert Sequence:

ATGCGGCTGG ACCGCCGGGC CCTCTATGCG CTAGTTCTGC TGCTTGCCTG CGCCTCGCTG 
GGTCTCCTGT ACGCCAGCAC CCGAGACGCG CCAGGTCTCC CGAACCCTCT GGCATTGTGG 
TCACCCCCAC AAGGTCCCCC GAGGCTCGAT CTGCTAGACC TTGCCACTGA GCCTCGCTAC 
GCACACATCC CAGTCAGGAT CAAGGAGCAA GTGGTGGGGC TGCTGGCTCA GAACAATTGC 
AGTTGTGAGT CCAGCGGAGG ACGCTTTGCC TTGCCGTTCC TGAGGCAGGT CCGGGCGATT 
GACTTCACTA AAGCCTTTGA CGCCGAGGAG CTGAGGGCTG TTTCTATCTC CAGAGAGCAG 
GAATACCAGG CCTTCCTTGC AAGGAGCCGG TCCCTGGCTG ACCAGCTGCT GATAGCCCCT 
GCCAACTCCC CCTTACAGTA TCCCCTGCAG GGTGTGGAGG TTCAGCCCCT CAGGAGCATC 
CTGGTGCCAG GGCTAAGTCT GCAGGAAGCT TCTGTTCAGG AAATATATCA GGTGAACCTG 
ATTGCTTCCC TTGGCACCTG GGATGTGGCA GGGGAAGTAA CAGGGGTGAC TCTCACTGGA 
GAGGGGCAGT CGGACCTCAC CCTTGCCAGC CCAATTCTGG ATAAACTCAA CCGACAGCTG 
CAACTGGTGA CTTACAGCAG CCGGAGCTAC CAAGCCAACA CAGCAGACAC AGTCCGGTTC 
TCCACCAAGG GACATGAAGT GGCCTTCACC ATCCTCATAA GACATCCTCC CAACCCCCGG 
CTGTACCCAC CATCATCCCT ACCCCAAGGA GCCCAGTACA ACATCAGTGC TCTGGTTACC 
GTTGCCACCA AGACCTTTCT TCGTTATGAT CGGCTACGGG CACTCATTGC CAGCATCAGA 
CGCTTTTACC CTACGGTCAC CATAGTAATC GCTGACGACA GCGACAAACC GGAGCGAATT 
AGCGACCCCC ATGTGGAGCA CTATTTCATG CCCTTCGGCA AGGGTTGGTT TGCAGGTCGG 
AACCTGGCGG TGTCCCAAGT AACCACCAAA TACGTGCTGT GGGTGGACGA CGACTTTGTC 
TTCACGGCGC GCACGCGGCT GGAGAAGCTT GTGGATGTCC TGGAGAGGAC GCCCCTGGAC 
TTGGTTGGGG GCGCGGTGCG GGAGATCTCG GGCTACGCTA CCACCTACCG ACAGCTGCTA 
AGTGTGGAGC CGGGCGCCCC AGGCTTTGGG AACTGCCTCC GGCAAAAGCA GGGCTTCCAC 
CACGAGCTCG CTGGCTTTCC AAACTGCGTG GTCACCGACG GCGTAGTCAA CTTCTTCCTG 
GCGCGCACAG ATAAAGTGCG CCAGGTGGGC TTTGACCCAC GCCTCAACCG GGTGGCTCAT 
CTGGAATTCT TCCTGGATGG TCTTGGTTCC CTTCGAGTTG GCTCCTGCTC TGATGTTGTT 
GTGGATCATG CGTCAAAGGT GAAGCTGCCT TGGACATCAA AGGATCCAGG GGCTGAACTT 
TATGCCCGTT ACCGTTACCC GGGATCACTG GACCAAAGTC AGGTGGCCAA ACATCGACTG 
CTCTTCTTCA AACACCGGCT ACAGTGCATG ACCGCCGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

B4galnt1 ( XM_017595100.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017450589.1 Rattus norvegicus beta-1,4-N-acetyl-galactosaminyl transferase 1 (B4galnt1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa06382
Clone ID Related Accession (Same CDS sequence)	NM_022860.2 , XM_017595100.1
Accession Version	XM_017595100.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	beta-1,4 N-acetylgalactosaminyltransferase 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCTGG ACCGCCGGGC CCTCTATGCG CTAGTTCTGC TGCTTGCCTG CGCCTCGCTG 
GGTCTCCTGT ACGCCAGCAC CCGAGACGCG CCAGGTCTCC CGAACCCTCT GGCATTGTGG 
TCACCCCCAC AAGGTCCCCC GAGGCTCGAT CTGCTAGACC TTGCCACTGA GCCTCGCTAC 
GCACACATCC CAGTCAGGAT CAAGGAGCAA GTGGTGGGGC TGCTGGCTCA GAACAATTGC 
AGTTGTGAGT CCAGCGGAGG ACGCTTTGCC TTGCCGTTCC TGAGGCAGGT CCGGGCGATT 
GACTTCACTA AAGCCTTTGA CGCCGAGGAG CTGAGGGCTG TTTCTATCTC CAGAGAGCAG 
GAATACCAGG CCTTCCTTGC AAGGAGCCGG TCCCTGGCTG ACCAGCTGCT GATAGCCCCT 
GCCAACTCCC CCTTACAGTA TCCCCTGCAG GGTGTGGAGG TTCAGCCCCT CAGGAGCATC 
CTGGTGCCAG GGCTAAGTCT GCAGGAAGCT TCTGTTCAGG AAATATATCA GGTGAACCTG 
ATTGCTTCCC TTGGCACCTG GGATGTGGCA GGGGAAGTAA CAGGGGTGAC TCTCACTGGA 
GAGGGGCAGT CGGACCTCAC CCTTGCCAGC CCAATTCTGG ATAAACTCAA CCGACAGCTG 
CAACTGGTGA CTTACAGCAG CCGGAGCTAC CAAGCCAACA CAGCAGACAC AGTCCGGTTC 
TCCACCAAGG GACATGAAGT GGCCTTCACC ATCCTCATAA GACATCCTCC CAACCCCCGG 
CTGTACCCAC CATCATCCCT ACCCCAAGGA GCCCAGTACA ACATCAGTGC TCTGGTTACC 
GTTGCCACCA AGACCTTTCT TCGTTATGAT CGGCTACGGG CACTCATTGC CAGCATCAGA 
CGCTTTTACC CTACGGTCAC CATAGTAATC GCTGACGACA GCGACAAACC GGAGCGAATT 
AGCGACCCCC ATGTGGAGCA CTATTTCATG CCCTTCGGCA AGGGTTGGTT TGCAGGTCGG 
AACCTGGCGG TGTCCCAAGT AACCACCAAA TACGTGCTGT GGGTGGACGA CGACTTTGTC 
TTCACGGCGC GCACGCGGCT GGAGAAGCTT GTGGATGTCC TGGAGAGGAC GCCCCTGGAC 
TTGGTTGGGG GCGCGGTGCG GGAGATCTCG GGCTACGCTA CCACCTACCG ACAGCTGCTA 
AGTGTGGAGC CGGGCGCCCC AGGCTTTGGG AACTGCCTCC GGCAAAAGCA GGGCTTCCAC 
CACGAGCTCG CTGGCTTTCC AAACTGCGTG GTCACCGACG GCGTAGTCAA CTTCTTCCTG 
GCGCGCACAG ATAAAGTGCG CCAGGTGGGC TTTGACCCAC GCCTCAACCG GGTGGCTCAT 
CTGGAATTCT TCCTGGATGG TCTTGGTTCC CTTCGAGTTG GCTCCTGCTC TGATGTTGTT 
GTGGATCATG CGTCAAAGGT GAAGCTGCCT TGGACATCAA AGGATCCAGG GGCTGAACTT 
TATGCCCGTT ACCGTTACCC GGGATCACTG GACCAAAGTC AGGTGGCCAA ACATCGACTG 
CTCTTCTTCA AACACCGGCT ACAGTGCATG ACCGCCGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017450589.1
CDS	21..1622
Translation	MRLDRRALYALVLLLACASLGLLYASTRDAPGLPNPLALWSPPQGPPRLDLLDLATEPRYAHIPVRIKEQVVGLLAQNNCSCESSGGRFALPFLRQVRAIDFTKAFDAEELRAVSISREQEYQAFLARSRSLADQLLIAPANSPLQYPLQGVEVQPLRSILVPGLSLQEASVQEIYQVNLIASLGTWDVAGEVTGVTLTGEGQSDLTLASPILDKLNRQLQLVTYSSRSYQANTADTVRFSTKGHEVAFTILIRHPPNPRLYPPSSLPQGAQYNISALVTVATKTFLRYDRLRALIASIRRFYPTVTIVIADDSDKPERISDPHVEHYFMPFGKGWFAGRNLAVSQVTTKYVLWVDDDFVFTARTRLEKLVDVLERTPLDLVGGAVREISGYATTYRQLLSVEPGAPGFGNCLRQKQGFHHELAGFPNCVVTDGVVNFFLARTDKVRQVGFDPRLNRVAHLEFFLDGLGSLRVGSCSDVVVDHASKVKLPWTSKDPGAELYARYRYPGSLDQSQVAKHRLLFFKHRLQCMTAE

Target ORF information:

RefSeq Version	XM_017595100.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus beta-1,4-N-acetyl-galactosaminyl transferase 1 (B4galnt1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017595100.1

ORF Insert Sequence:

ATGCGGCTGG ACCGCCGGGC CCTCTATGCG CTAGTTCTGC TGCTTGCCTG CGCCTCGCTG 
GGTCTCCTGT ACGCCAGCAC CCGAGACGCG CCAGGTCTCC CGAACCCTCT GGCATTGTGG 
TCACCCCCAC AAGGTCCCCC GAGGCTCGAT CTGCTAGACC TTGCCACTGA GCCTCGCTAC 
GCACACATCC CAGTCAGGAT CAAGGAGCAA GTGGTGGGGC TGCTGGCTCA GAACAATTGC 
AGTTGTGAGT CCAGCGGAGG ACGCTTTGCC TTGCCGTTCC TGAGGCAGGT CCGGGCGATT 
GACTTCACTA AAGCCTTTGA CGCCGAGGAG CTGAGGGCTG TTTCTATCTC CAGAGAGCAG 
GAATACCAGG CCTTCCTTGC AAGGAGCCGG TCCCTGGCTG ACCAGCTGCT GATAGCCCCT 
GCCAACTCCC CCTTACAGTA TCCCCTGCAG GGTGTGGAGG TTCAGCCCCT CAGGAGCATC 
CTGGTGCCAG GGCTAAGTCT GCAGGAAGCT TCTGTTCAGG AAATATATCA GGTGAACCTG 
ATTGCTTCCC TTGGCACCTG GGATGTGGCA GGGGAAGTAA CAGGGGTGAC TCTCACTGGA 
GAGGGGCAGT CGGACCTCAC CCTTGCCAGC CCAATTCTGG ATAAACTCAA CCGACAGCTG 
CAACTGGTGA CTTACAGCAG CCGGAGCTAC CAAGCCAACA CAGCAGACAC AGTCCGGTTC 
TCCACCAAGG GACATGAAGT GGCCTTCACC ATCCTCATAA GACATCCTCC CAACCCCCGG 
CTGTACCCAC CATCATCCCT ACCCCAAGGA GCCCAGTACA ACATCAGTGC TCTGGTTACC 
GTTGCCACCA AGACCTTTCT TCGTTATGAT CGGCTACGGG CACTCATTGC CAGCATCAGA 
CGCTTTTACC CTACGGTCAC CATAGTAATC GCTGACGACA GCGACAAACC GGAGCGAATT 
AGCGACCCCC ATGTGGAGCA CTATTTCATG CCCTTCGGCA AGGGTTGGTT TGCAGGTCGG 
AACCTGGCGG TGTCCCAAGT AACCACCAAA TACGTGCTGT GGGTGGACGA CGACTTTGTC 
TTCACGGCGC GCACGCGGCT GGAGAAGCTT GTGGATGTCC TGGAGAGGAC GCCCCTGGAC 
TTGGTTGGGG GCGCGGTGCG GGAGATCTCG GGCTACGCTA CCACCTACCG ACAGCTGCTA 
AGTGTGGAGC CGGGCGCCCC AGGCTTTGGG AACTGCCTCC GGCAAAAGCA GGGCTTCCAC 
CACGAGCTCG CTGGCTTTCC AAACTGCGTG GTCACCGACG GCGTAGTCAA CTTCTTCCTG 
GCGCGCACAG ATAAAGTGCG CCAGGTGGGC TTTGACCCAC GCCTCAACCG GGTGGCTCAT 
CTGGAATTCT TCCTGGATGG TCTTGGTTCC CTTCGAGTTG GCTCCTGCTC TGATGTTGTT 
GTGGATCATG CGTCAAAGGT GAAGCTGCCT TGGACATCAA AGGATCCAGG GGCTGAACTT 
TATGCCCGTT ACCGTTACCC GGGATCACTG GACCAAAGTC AGGTGGCCAA ACATCGACTG 
CTCTTCTTCA AACACCGGCT ACAGTGCATG ACCGCCGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

B4galnt1 ( XM_008765420.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008763642.1 Rattus norvegicus beta-1,4-N-acetyl-galactosaminyl transferase 1 (B4galnt1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa30277
Clone ID Related Accession (Same CDS sequence)	XM_008765420.2
Accession Version	XM_008765420.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1710bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	beta-1,4 N-acetylgalactosaminyltransferase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008765420.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGGCC TTGGGTACCT GAGCGAGTCT CGGAGGCGGA GGGGTCCACA GCCTGGGCCC 
TCATCTTCCC TCAGCGCCTG GTCACCTCTC ACCCTCCCCC ACCTCAGGAT GCGGCTGGAC 
CGCCGGGCCC TCTATGCGCT AGTTCTGCTG CTTGCCTGCG CCTCGCTGGG TCTCCTGTAC 
GCCAGCACCC GAGACGCGCC AGGTCTCCCG AACCCTCTGG CATTGTGGTC ACCCCCACAA 
GGTCCCCCGA GGCTCGATCT GCTAGACCTT GCCACTGAGC CTCGCTACGC ACACATCCCA 
GTCAGGATCA AGGAGCAAGT GGTGGGGCTG CTGGCTCAGA ACAATTGCAG TTGTGAGTCC 
AGCGGAGGAC GCTTTGCCTT GCCGTTCCTG AGGCAGGTCC GGGCGATTGA CTTCACTAAA 
GCCTTTGACG CCGAGGAGCT GAGGGCTGTT TCTATCTCCA GAGAGCAGGA ATACCAGGCC 
TTCCTTGCAA GGAGCCGGTC CCTGGCTGAC CAGCTGCTGA TAGCCCCTGC CAACTCCCCC 
TTACAGTATC CCCTGCAGGG TGTGGAGGTT CAGCCCCTCA GGAGCATCCT GGTGCCAGGG 
CTAAGTCTGC AGGAAGCTTC TGTTCAGGAA ATATATCAGG TGAACCTGAT TGCTTCCCTT 
GGCACCTGGG ATGTGGCAGG GGAAGTAACA GGGGTGACTC TCACTGGAGA GGGGCAGTCG 
GACCTCACCC TTGCCAGCCC AATTCTGGAT AAACTCAACC GACAGCTGCA ACTGGTGACT 
TACAGCAGCC GGAGCTACCA AGCCAACACA GCAGACACAG TCCGGTTCTC CACCAAGGGA 
CATGAAGTGG CCTTCACCAT CCTCATAAGA CATCCTCCCA ACCCCCGGCT GTACCCACCA 
TCATCCCTAC CCCAAGGAGC CCAGTACAAC ATCAGTGCTC TGGTTACCGT TGCCACCAAG 
ACCTTTCTTC GTTATGATCG GCTACGGGCA CTCATTGCCA GCATCAGACG CTTTTACCCT 
ACGGTCACCA TAGTAATCGC TGACGACAGC GACAAACCGG AGCGAATTAG CGACCCCCAT 
GTGGAGCACT ATTTCATGCC CTTCGGCAAG GGTTGGTTTG CAGGTCGGAA CCTGGCGGTG 
TCCCAAGTAA CCACCAAATA CGTGCTGTGG GTGGACGACG ACTTTGTCTT CACGGCGCGC 
ACGCGGCTGG AGAAGCTTGT GGATGTCCTG GAGAGGACGC CCCTGGACTT GGTTGGGGGC 
GCGGTGCGGG AGATCTCGGG CTACGCTACC ACCTACCGAC AGCTGCTAAG TGTGGAGCCG 
GGCGCCCCAG GCTTTGGGAA CTGCCTCCGG CAAAAGCAGG GCTTCCACCA CGAGCTCGCT 
GGCTTTCCAA ACTGCGTGGT CACCGACGGC GTAGTCAACT TCTTCCTGGC GCGCACAGAT 
AAAGTGCGCC AGGTGGGCTT TGACCCACGC CTCAACCGGG TGGCTCATCT GGAATTCTTC 
CTGGATGGTC TTGGTTCCCT TCGAGTTGGC TCCTGCTCTG ATGTTGTTGT GGATCATGCG 
TCAAAGGTGA AGCTGCCTTG GACATCAAAG GATCCAGGGG CTGAACTTTA TGCCCGTTAC 
CGTTACCCGG GATCACTGGA CCAAAGTCAG GTGGCCAAAC ATCGACTGCT CTTCTTCAAA 
CACCGGCTAC AGTGCATGAC CGCCGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008763642.1
CDS	709..2418
Translation	MDGLGYLSESRRRRGPQPGPSSSLSAWSPLTLPHLRMRLDRRALYALVLLLACASLGLLYASTRDAPGLPNPLALWSPPQGPPRLDLLDLATEPRYAHIPVRIKEQVVGLLAQNNCSCESSGGRFALPFLRQVRAIDFTKAFDAEELRAVSISREQEYQAFLARSRSLADQLLIAPANSPLQYPLQGVEVQPLRSILVPGLSLQEASVQEIYQVNLIASLGTWDVAGEVTGVTLTGEGQSDLTLASPILDKLNRQLQLVTYSSRSYQANTADTVRFSTKGHEVAFTILIRHPPNPRLYPPSSLPQGAQYNISALVTVATKTFLRYDRLRALIASIRRFYPTVTIVIADDSDKPERISDPHVEHYFMPFGKGWFAGRNLAVSQVTTKYVLWVDDDFVFTARTRLEKLVDVLERTPLDLVGGAVREISGYATTYRQLLSVEPGAPGFGNCLRQKQGFHHELAGFPNCVVTDGVVNFFLARTDKVRQVGFDPRLNRVAHLEFFLDGLGSLRVGSCSDVVVDHASKVKLPWTSKDPGAELYARYRYPGSLDQSQVAKHRLLFFKHRLQCMTAE

Target ORF information:

RefSeq Version	XM_008765420.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus beta-1,4-N-acetyl-galactosaminyl transferase 1 (B4galnt1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008765420.2

ORF Insert Sequence:

ATGGATGGCC TTGGGTACCT GAGCGAGTCT CGGAGGCGGA GGGGTCCACA GCCTGGGCCC 
TCATCTTCCC TCAGCGCCTG GTCACCTCTC ACCCTCCCCC ACCTCAGGAT GCGGCTGGAC 
CGCCGGGCCC TCTATGCGCT AGTTCTGCTG CTTGCCTGCG CCTCGCTGGG TCTCCTGTAC 
GCCAGCACCC GAGACGCGCC AGGTCTCCCG AACCCTCTGG CATTGTGGTC ACCCCCACAA 
GGTCCCCCGA GGCTCGATCT GCTAGACCTT GCCACTGAGC CTCGCTACGC ACACATCCCA 
GTCAGGATCA AGGAGCAAGT GGTGGGGCTG CTGGCTCAGA ACAATTGCAG TTGTGAGTCC 
AGCGGAGGAC GCTTTGCCTT GCCGTTCCTG AGGCAGGTCC GGGCGATTGA CTTCACTAAA 
GCCTTTGACG CCGAGGAGCT GAGGGCTGTT TCTATCTCCA GAGAGCAGGA ATACCAGGCC 
TTCCTTGCAA GGAGCCGGTC CCTGGCTGAC CAGCTGCTGA TAGCCCCTGC CAACTCCCCC 
TTACAGTATC CCCTGCAGGG TGTGGAGGTT CAGCCCCTCA GGAGCATCCT GGTGCCAGGG 
CTAAGTCTGC AGGAAGCTTC TGTTCAGGAA ATATATCAGG TGAACCTGAT TGCTTCCCTT 
GGCACCTGGG ATGTGGCAGG GGAAGTAACA GGGGTGACTC TCACTGGAGA GGGGCAGTCG 
GACCTCACCC TTGCCAGCCC AATTCTGGAT AAACTCAACC GACAGCTGCA ACTGGTGACT 
TACAGCAGCC GGAGCTACCA AGCCAACACA GCAGACACAG TCCGGTTCTC CACCAAGGGA 
CATGAAGTGG CCTTCACCAT CCTCATAAGA CATCCTCCCA ACCCCCGGCT GTACCCACCA 
TCATCCCTAC CCCAAGGAGC CCAGTACAAC ATCAGTGCTC TGGTTACCGT TGCCACCAAG 
ACCTTTCTTC GTTATGATCG GCTACGGGCA CTCATTGCCA GCATCAGACG CTTTTACCCT 
ACGGTCACCA TAGTAATCGC TGACGACAGC GACAAACCGG AGCGAATTAG CGACCCCCAT 
GTGGAGCACT ATTTCATGCC CTTCGGCAAG GGTTGGTTTG CAGGTCGGAA CCTGGCGGTG 
TCCCAAGTAA CCACCAAATA CGTGCTGTGG GTGGACGACG ACTTTGTCTT CACGGCGCGC 
ACGCGGCTGG AGAAGCTTGT GGATGTCCTG GAGAGGACGC CCCTGGACTT GGTTGGGGGC 
GCGGTGCGGG AGATCTCGGG CTACGCTACC ACCTACCGAC AGCTGCTAAG TGTGGAGCCG 
GGCGCCCCAG GCTTTGGGAA CTGCCTCCGG CAAAAGCAGG GCTTCCACCA CGAGCTCGCT 
GGCTTTCCAA ACTGCGTGGT CACCGACGGC GTAGTCAACT TCTTCCTGGC GCGCACAGAT 
AAAGTGCGCC AGGTGGGCTT TGACCCACGC CTCAACCGGG TGGCTCATCT GGAATTCTTC 
CTGGATGGTC TTGGTTCCCT TCGAGTTGGC TCCTGCTCTG ATGTTGTTGT GGATCATGCG 
TCAAAGGTGA AGCTGCCTTG GACATCAAAG GATCCAGGGG CTGAACTTTA TGCCCGTTAC 
CGTTACCCGG GATCACTGGA CCAAAGTCAG GTGGCCAAAC ATCGACTGCT CTTCTTCAAA 
CACCGGCTAC AGTGCATGAC CGCCGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

B4galnt1 ( XM_006241497.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006241559.1 Rattus norvegicus beta-1,4-N-acetyl-galactosaminyl transferase 1 (B4galnt1), transcript variant X2, mRNA.

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CloneID	ORa30278
Clone ID Related Accession (Same CDS sequence)	XM_006241497.3
Accession Version	XM_006241497.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1665bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	beta-1,4 N-acetylgalactosaminyltransferase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006241497.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGAAGC CCAGAGGCGA GAGAGAGAGA GGCGGAAGAA AGGAGGCCGG GAGACCTTTG 
GGGATGCGGC TGGACCGCCG GGCCCTCTAT GCGCTAGTTC TGCTGCTTGC CTGCGCCTCG 
CTGGGTCTCC TGTACGCCAG CACCCGAGAC GCGCCAGGTC TCCCGAACCC TCTGGCATTG 
TGGTCACCCC CACAAGGTCC CCCGAGGCTC GATCTGCTAG ACCTTGCCAC TGAGCCTCGC 
TACGCACACA TCCCAGTCAG GATCAAGGAG CAAGTGGTGG GGCTGCTGGC TCAGAACAAT 
TGCAGTTGTG AGTCCAGCGG AGGACGCTTT GCCTTGCCGT TCCTGAGGCA GGTCCGGGCG 
ATTGACTTCA CTAAAGCCTT TGACGCCGAG GAGCTGAGGG CTGTTTCTAT CTCCAGAGAG 
CAGGAATACC AGGCCTTCCT TGCAAGGAGC CGGTCCCTGG CTGACCAGCT GCTGATAGCC 
CCTGCCAACT CCCCCTTACA GTATCCCCTG CAGGGTGTGG AGGTTCAGCC CCTCAGGAGC 
ATCCTGGTGC CAGGGCTAAG TCTGCAGGAA GCTTCTGTTC AGGAAATATA TCAGGTGAAC 
CTGATTGCTT CCCTTGGCAC CTGGGATGTG GCAGGGGAAG TAACAGGGGT GACTCTCACT 
GGAGAGGGGC AGTCGGACCT CACCCTTGCC AGCCCAATTC TGGATAAACT CAACCGACAG 
CTGCAACTGG TGACTTACAG CAGCCGGAGC TACCAAGCCA ACACAGCAGA CACAGTCCGG 
TTCTCCACCA AGGGACATGA AGTGGCCTTC ACCATCCTCA TAAGACATCC TCCCAACCCC 
CGGCTGTACC CACCATCATC CCTACCCCAA GGAGCCCAGT ACAACATCAG TGCTCTGGTT 
ACCGTTGCCA CCAAGACCTT TCTTCGTTAT GATCGGCTAC GGGCACTCAT TGCCAGCATC 
AGACGCTTTT ACCCTACGGT CACCATAGTA ATCGCTGACG ACAGCGACAA ACCGGAGCGA 
ATTAGCGACC CCCATGTGGA GCACTATTTC ATGCCCTTCG GCAAGGGTTG GTTTGCAGGT 
CGGAACCTGG CGGTGTCCCA AGTAACCACC AAATACGTGC TGTGGGTGGA CGACGACTTT 
GTCTTCACGG CGCGCACGCG GCTGGAGAAG CTTGTGGATG TCCTGGAGAG GACGCCCCTG 
GACTTGGTTG GGGGCGCGGT GCGGGAGATC TCGGGCTACG CTACCACCTA CCGACAGCTG 
CTAAGTGTGG AGCCGGGCGC CCCAGGCTTT GGGAACTGCC TCCGGCAAAA GCAGGGCTTC 
CACCACGAGC TCGCTGGCTT TCCAAACTGC GTGGTCACCG ACGGCGTAGT CAACTTCTTC 
CTGGCGCGCA CAGATAAAGT GCGCCAGGTG GGCTTTGACC CACGCCTCAA CCGGGTGGCT 
CATCTGGAAT TCTTCCTGGA TGGTCTTGGT TCCCTTCGAG TTGGCTCCTG CTCTGATGTT 
GTTGTGGATC ATGCGTCAAA GGTGAAGCTG CCTTGGACAT CAAAGGATCC AGGGGCTGAA 
CTTTATGCCC GTTACCGTTA CCCGGGATCA CTGGACCAAA GTCAGGTGGC CAAACATCGA 
CTGCTCTTCT TCAAACACCG GCTACAGTGC ATGACCGCCG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006241559.1
CDS	604..2268
Translation	MRKPRGERERGGRKEAGRPLGMRLDRRALYALVLLLACASLGLLYASTRDAPGLPNPLALWSPPQGPPRLDLLDLATEPRYAHIPVRIKEQVVGLLAQNNCSCESSGGRFALPFLRQVRAIDFTKAFDAEELRAVSISREQEYQAFLARSRSLADQLLIAPANSPLQYPLQGVEVQPLRSILVPGLSLQEASVQEIYQVNLIASLGTWDVAGEVTGVTLTGEGQSDLTLASPILDKLNRQLQLVTYSSRSYQANTADTVRFSTKGHEVAFTILIRHPPNPRLYPPSSLPQGAQYNISALVTVATKTFLRYDRLRALIASIRRFYPTVTIVIADDSDKPERISDPHVEHYFMPFGKGWFAGRNLAVSQVTTKYVLWVDDDFVFTARTRLEKLVDVLERTPLDLVGGAVREISGYATTYRQLLSVEPGAPGFGNCLRQKQGFHHELAGFPNCVVTDGVVNFFLARTDKVRQVGFDPRLNRVAHLEFFLDGLGSLRVGSCSDVVVDHASKVKLPWTSKDPGAELYARYRYPGSLDQSQVAKHRLLFFKHRLQCMTAE

Target ORF information:

RefSeq Version	XM_006241497.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus beta-1,4-N-acetyl-galactosaminyl transferase 1 (B4galnt1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006241497.3

ORF Insert Sequence:

ATGCGGAAGC CCAGAGGCGA GAGAGAGAGA GGCGGAAGAA AGGAGGCCGG GAGACCTTTG 
GGGATGCGGC TGGACCGCCG GGCCCTCTAT GCGCTAGTTC TGCTGCTTGC CTGCGCCTCG 
CTGGGTCTCC TGTACGCCAG CACCCGAGAC GCGCCAGGTC TCCCGAACCC TCTGGCATTG 
TGGTCACCCC CACAAGGTCC CCCGAGGCTC GATCTGCTAG ACCTTGCCAC TGAGCCTCGC 
TACGCACACA TCCCAGTCAG GATCAAGGAG CAAGTGGTGG GGCTGCTGGC TCAGAACAAT 
TGCAGTTGTG AGTCCAGCGG AGGACGCTTT GCCTTGCCGT TCCTGAGGCA GGTCCGGGCG 
ATTGACTTCA CTAAAGCCTT TGACGCCGAG GAGCTGAGGG CTGTTTCTAT CTCCAGAGAG 
CAGGAATACC AGGCCTTCCT TGCAAGGAGC CGGTCCCTGG CTGACCAGCT GCTGATAGCC 
CCTGCCAACT CCCCCTTACA GTATCCCCTG CAGGGTGTGG AGGTTCAGCC CCTCAGGAGC 
ATCCTGGTGC CAGGGCTAAG TCTGCAGGAA GCTTCTGTTC AGGAAATATA TCAGGTGAAC 
CTGATTGCTT CCCTTGGCAC CTGGGATGTG GCAGGGGAAG TAACAGGGGT GACTCTCACT 
GGAGAGGGGC AGTCGGACCT CACCCTTGCC AGCCCAATTC TGGATAAACT CAACCGACAG 
CTGCAACTGG TGACTTACAG CAGCCGGAGC TACCAAGCCA ACACAGCAGA CACAGTCCGG 
TTCTCCACCA AGGGACATGA AGTGGCCTTC ACCATCCTCA TAAGACATCC TCCCAACCCC 
CGGCTGTACC CACCATCATC CCTACCCCAA GGAGCCCAGT ACAACATCAG TGCTCTGGTT 
ACCGTTGCCA CCAAGACCTT TCTTCGTTAT GATCGGCTAC GGGCACTCAT TGCCAGCATC 
AGACGCTTTT ACCCTACGGT CACCATAGTA ATCGCTGACG ACAGCGACAA ACCGGAGCGA 
ATTAGCGACC CCCATGTGGA GCACTATTTC ATGCCCTTCG GCAAGGGTTG GTTTGCAGGT 
CGGAACCTGG CGGTGTCCCA AGTAACCACC AAATACGTGC TGTGGGTGGA CGACGACTTT 
GTCTTCACGG CGCGCACGCG GCTGGAGAAG CTTGTGGATG TCCTGGAGAG GACGCCCCTG 
GACTTGGTTG GGGGCGCGGT GCGGGAGATC TCGGGCTACG CTACCACCTA CCGACAGCTG 
CTAAGTGTGG AGCCGGGCGC CCCAGGCTTT GGGAACTGCC TCCGGCAAAA GCAGGGCTTC 
CACCACGAGC TCGCTGGCTT TCCAAACTGC GTGGTCACCG ACGGCGTAGT CAACTTCTTC 
CTGGCGCGCA CAGATAAAGT GCGCCAGGTG GGCTTTGACC CACGCCTCAA CCGGGTGGCT 
CATCTGGAAT TCTTCCTGGA TGGTCTTGGT TCCCTTCGAG TTGGCTCCTG CTCTGATGTT 
GTTGTGGATC ATGCGTCAAA GGTGAAGCTG CCTTGGACAT CAAAGGATCC AGGGGCTGAA 
CTTTATGCCC GTTACCGTTA CCCGGGATCA CTGGACCAAA GTCAGGTGGC CAAACATCGA 
CTGCTCTTCT TCAAACACCG GCTACAGTGC ATGACCGCCG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Male germ cells require polyenoic sphingolipids with complex glycosylation for completion of meiosis: a link to ceramide synthase-3.
The Journal of biological chemistry283(19)13357-69(2008 May)
Rabionet M,van der Spoel AC,Chuang CC,von Tümpling-Radosta B,Litjens M,Bouwmeester D,Hellbusch CC,Körner C,Wiegandt H,Gorgas K,Platt FM,Gröne HJ,Sandhoff R

A genetic model of substrate deprivation therapy for a glycosphingolipid storage disorder.
The Journal of clinical investigation103(4)497-505(1999 Feb)
Liu Y,Wada R,Kawai H,Sango K,Deng C,Tai T,McDonald MP,Araujo K,Crawley JN,Bierfreund U,Sandhoff K,Suzuki K,Proia RL

Mice with disrupted GM2/GD2 synthase gene lack complex gangliosides but exhibit only subtle defects in their nervous system.
Proceedings of the National Academy of Sciences of the United States of America93(20)10662-7(1996 Oct)
Takamiya K,Yamamoto A,Furukawa K,Yamashiro S,Shin M,Okada M,Fukumoto S,Haraguchi M,Takeda N,Fujimura K,Sakae M,Kishikawa M,Shiku H,Furukawa K,Aizawa S

beta 1-4N-acetylgalactosaminyltransferase can synthesize both asialoglycosphingolipid GM2 and glycosphingolipid GM2 in vitro and in vivo: isolation and characterization of a beta 1-4N-acetylgalactosaminyltransferase cDNA clone from rat ascites hepatoma cell line AH7974F.
The Biochemical journal303 ( Pt 3)957-65(1994 Nov)
Hidari JK,Ichikawa S,Furukawa K,Yamasaki M,Hirabayashi Y

Expression cloning of beta 1,4 N-acetylgalactosaminyltransferase cDNAs that determine the expression of GM2 and GD2 gangliosides.
The Journal of biological chemistry267(17)12082-9(1992 Jun)
Nagata Y,Yamashiro S,Yodoi J,Lloyd KO,Shiku H,Furukawa K

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B4galnt1 cDNA ORF clone, Rattus norvegicus(Norway rat)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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