Safb cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Safb
Entrez Gene ID	64196
Full Name	scaffold attachment factor B
General protein information	Preferred Names scaffold attachment factor B Names scaffold attachment factor B1 SAF-B SAF-B1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 9 Map Location: 9q12
Summary	interacts with RNA polymerase II and serine-arginine-rich RNA processing factors; may be a molecular base to assemble the transcriptosome complex [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_022394.2	NP_071789.2	scaffold attachment factor B1
XM_017596622.1	XP_017452111.1	scaffold attachment factor B1 isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Safb	NP_071789.1
Pan troglodytes (chimpanzee)	SAFB	XP_001142439.3
Gallus gallus (chicken)	SAFB	XP_423726.3
Homo sapiens (human)	SAFB	NP_001188267.1
Mus musculus (house mouse)	Safb	NP_001156772.1
Gallus gallus (chicken)	SAFB2	XP_004948787.1
Macaca mulatta (Rhesus monkey)	SAFB	XP_001085118.2
Canis lupus familiaris (dog)	SAFB	XP_005633287.1
Danio rerio (zebrafish)	safb	NP_999848.1
Bos taurus (cattle)	SAFB	NP_001093185.1

Gene Ontology
Bibliography

Related articles in PubMed

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanhäusser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

Heterogeneous nuclear ribonucleoprotein G regulates splice site selection by binding to CC(A/C)-rich regions in pre-mRNA.
Heinrich B, Zhang Z, Raitskin O, Hiller M, Benderska N, Hartmann AM, Bracco L, Elliott D, Ben-Ari S, Soreq H, Sperling J, Sperling R, Stamm S
The Journal of biological chemistry284(21)14303-15(2009 May)

SAFB1, an RBMX-binding protein, is a newly identified regulator of hepatic SREBP-1c gene.
Omura Y, Nishio Y, Takemoto T, Ikeuchi C, Sekine O, Morino K, Maeno Y, Obata T, Ugi S, Maegawa H, Kimura H, Kashiwagi A
BMB reports42(4)232-7(2009 Apr)

Phosphoproteomic analysis of rat liver by high capacity IMAC and LC-MS/MS.
Moser K, White FM
Journal of proteome research5(1)98-104(2006 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Safb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Safb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa43570	NM_022394.2 Latest version!	Rattus norvegicus scaffold attachment factor B (Safb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ORa62851	XM_017596622.1 Latest version!	Rattus norvegicus scaffold attachment factor B (Safb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Safb ( NM_022394.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_071789.2 Rattus norvegicus scaffold attachment factor B (Safb), mRNA.

Price & Availability↑

CloneID	ORa43570
Clone ID Related Accession (Same CDS sequence)	NM_022394.2
Accession Version	NM_022394.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2823bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562256000000
Organism	Rattus norvegicus(Norway rat)
Product	scaffold attachment factor B1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07066522.1. On Apr 30, 2015 this sequence version replaced NM_022394.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF056324.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGAGA CTCTGTCGGG TTTAGGAGAC TCGGGTGCGG CAAGCGCAGC GGCCGTGAGC 
TCCGCGGCGT CAGAGACCGG GACGCGGCGG CTCAGCGACC TGCGGGTGAT CGACCTGCGG 
GCGGAGCTGC GGAAGCGAAA CCTCGACTCG AGCGGCAACA AGAGCGTGCT GATGGAGCGG 
CTTAAGAAGG CCATTGAAGA GGAAGGTGGG AATCCTGATG AAATTGAAGT CATCTCAGAA 
GGCAACAAGA AGATGCCTAA GAGGCCCAGC AAAGGGAAGA AACCAGAAGA CGAGGGTGTG 
GAGGACAACG GGCTCGAGGA GAATTCTGGG GATGGGCAGG AGGATGTGGA AACCAGCCTG 
GAGAACCTGC AAGACATGGA TATGATGGAC ATCAGTGTGC TGGATGAGGC TGACATTGAT 
AATGGCAGCG TGGCAGACTG TGTGGAGGAA GAAGAGGAGG CCACACTCCC TGAGGGCCTA 
GGAGACAGCA CTGAACTGGT AGAAGGCGAC TCGAAGGGGC TCCCAGAACA GCTGCAGGAG 
CATGCTATAG ATGACAAGGA AGCCATTAAC AATGTAGATA CATCATCATC TGACTTCACC 
ATCCTGCAGG AGATGGAAGA GGCATCTTTG GAGCCAGAAA ATGAGAAAAT ACTCGACATT 
TTGGGGGAAA CTTGTAAATC TGAGCCAGTA AAAGAAGAAG GTTCGGAGCT GGAGCAGCCC 
TTTGCCCAGG CCACGAGTAG CGTGGGGCCA GACAGGAAGC TGGCGGAGGA AGAGGACCTA 
TTTGAGAGCT GCGGCCACCC GGAAGAGGAA GAAGAAGAGG AGGAAGAAGA GGAGCAGGAA 
GAGGAGCAGG AGGAGGAGGG AGATTTAGCT TTGGCCAGCA GCAGCAAGTC TGAGTCTTCA 
AGCACTCGAT GTCAGTGGAG CGAGGCAGAC GCCCTGTTAG CAGTAGTGAA AAGGGAGCCG 
GCAGAGGCGC CTGGCGGAGG CACTGGGATG GACCGCGAGC CTGTAGGGCT AGAGGAGCCA 
GTTGAACAGA GTAGCACGGC TGCCCAGCTC CCGGAGACCA CCAGCCAGGA GCTGGTGCGA 
GCGCCCACGG CAGCCCCGAG CCCTGAGCCC CGAGATAGCA AAGACGACGT GAAGAAGTTT 
GCTTTTGACG CTTGTAATGA CGTCCCTGCG GCTCCTAAAG AGTCCTCAGC CAGTGAGGGC 
GCTGATCAGA AAATGAGTTC TGTGGAAGAT GACTCGGATA CAAAAAGGCT TTCCAGAGAG 
GAGAAGGGTC GCAGCAGCTG TGGTAGGAAT TTCTGGGTTA GTGGCCTTTC ATCTACGACC 
AGAGCCACAG ATCTGAAGAA CCTTTTTAGC AGATACGGGA AGGTGGTGGG AGCTAAGGTT 
GTGACGAACG CCCGGAGTCC CGGAGCTCGC TGTTACGGCT TTGTCACTAT GTCCACAGCT 
GAAGAGGCAA CAAAGTGCAT TAACCATTTG CATAAGACTG AGCTGCATGG CAAGATGATC 
TCGGTGGAAA AGGCCAAAAG TGAGCCCGCT GGCAAAAGAG TACCTGACAG GCGAGATGGC 
GACAGCAAGA AAGAGAAGAC CAGCACCAGT GACAGATCTG CAAACCTTAA GAGGGAGGAG 
AAAGGTGACA GAAAAGACGA TGCCAAGAAG ACGGATGATG GGAGCACAGA GAAGAGCAAG 
GATGCAGATG ACCAAAAGCC TGGGCCTTCA GAGCGCTCTC GAACAACCAA GTCAGGAAGC 
CGTGGCACTG AGCGTACTGT GGTGATGGAC AAGTCCAAAG GTGTGCCTGT CATCAGTGTG 
AAGACTTCTG GTTCTAAAGA GAGAGCCTCC AAAAGTCAGG ACCGAAAGTC TGTCAGCCGG 
GAGAAGCGGT CTGTTGTGTC CTTTGACAAG GTTAAAGAGT CTCGAAAGTC CAGAGACTCG 
GAGTCACGGA GGGAGCGGGA GCGGGAGCGC AGTGAGCGGG AGCAAAGGCT GCAGGCCCAG 
TGGGAGCGGG AGGAGCGAGA GCGGCTGGAG ATTGCCCGGG AGCGCCTGGC TTTCCACCGA 
CATAGGCTGG AGCGTGAACG CATGGAGAGG GAACGGTTGG AGCGGGAGCG CATGCATGTG 
GAACAGGAGC GGAGGCGTGA GCAAGAGCGC ATTCACCGAG AGCGAGAGGA GCTTCGGCGT 
CAGCAGGAAC TGCGCTATGA ACAGGAGCGG AGGCCTGCTG TGCGCAGGCC CTATGAAGTA 
GATGGCCGGC GAGATGATGC CTATTGGCCA GAAGCTAAAC GGGCTGCGCT GGATGACCGT 
TACCACTCAG ACTTCAGTCG CCAGGACCGC TTTCATGACT TCGACCACAG AGACAGAGGC 
CGCTATCCCA ACCACTCTGT GGATAGGAGA GAAGGTTCAC GGTCGATGAT GGGAGACAGA 
GAAGGACAGC ATTACCCTGA GCGCCATGGA GGACCTGAAC GCCATGGCCG GGACTCTCGA 
GATGGCTGGG GCTATGGCTC AAACAAGAGA CTGAGTGAAG GCCGGGGGCT GCCCCCTCCT 
CCCAGGCGTG ACTGGGGAGA GCATGGCCGG AGACTTGAAG ATGACCGGGC ATGGCAGGGC 
ACAGCTGATG GAGGCATGAT GGAGCGTGAT CACAAGAGAT GGCAAGGTGG TGAGAGGAGC 
ATGTCTGGCC ACTCAGGGCC CGGTCACATG ATGAATCGTG GTGGCATGTC AGGGCGAGGC 
AGTTTTGCCC CCGGAGGAGC TTCCCGTGGC CATGTGATCC CACGTGGAGG GATGCAGGCA 
GGCTTCGGCG GACAGAGCCG GGGCAGCAGA CCCAGTGATG CCCGCTTCAC CCGCCGCTAT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071789.2
CDS	95..2917
Translation	MAETLSGLGDSGAASAAAVSSAASETGTRRLSDLRVIDLRAELRKRNLDSSGNKSVLMERLKKAIEEEGGNPDEIEVISEGNKKMPKRPSKGKKPEDEGVEDNGLEENSGDGQEDVETSLENLQDMDMMDISVLDEADIDNGSVADCVEEEEEATLPEGLGDSTELVEGDSKGLPEQLQEHAIDDKEAINNVDTSSSDFTILQEMEEASLEPENEKILDILGETCKSEPVKEEGSELEQPFAQATSSVGPDRKLAEEEDLFESCGHPEEEEEEEEEEEQEEEQEEEGDLALASSSKSESSSTRCQWSEADALLAVVKREPAEAPGGGTGMDREPVGLEEPVEQSSTAAQLPETTSQELVRAPTAAPSPEPRDSKDDVKKFAFDACNDVPAAPKESSASEGADQKMSSVEDDSDTKRLSREEKGRSSCGRNFWVSGLSSTTRATDLKNLFSRYGKVVGAKVVTNARSPGARCYGFVTMSTAEEATKCINHLHKTELHGKMISVEKAKSEPAGKRVPDRRDGDSKKEKTSTSDRSANLKREEKGDRKDDAKKTDDGSTEKSKDADDQKPGPSERSRTTKSGSRGTERTVVMDKSKGVPVISVKTSGSKERASKSQDRKSVSREKRSVVSFDKVKESRKSRDSESRRERERERSEREQRLQAQWEREERERLEIARERLAFHRHRLERERMERERLERERMHVEQERRREQERIHREREELRRQQELRYEQERRPAVRRPYEVDGRRDDAYWPEAKRAALDDRYHSDFSRQDRFHDFDHRDRGRYPNHSVDRREGSRSMMGDREGQHYPERHGGPERHGRDSRDGWGYGSNKRLSEGRGLPPPPRRDWGEHGRRLEDDRAWQGTADGGMMERDHKRWQGGERSMSGHSGPGHMMNRGGMSGRGSFAPGGASRGHVIPRGGMQAGFGGQSRGSRPSDARFTRRY

Target ORF information:

RefSeq Version	NM_022394.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus scaffold attachment factor B (Safb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022394.2

ORF Insert Sequence:

ATGGCGGAGA CTCTGTCGGG TTTAGGAGAC TCGGGTGCGG CAAGCGCAGC GGCCGTGAGC 
TCCGCGGCGT CAGAGACCGG GACGCGGCGG CTCAGCGACC TGCGGGTGAT CGACCTGCGG 
GCGGAGCTGC GGAAGCGAAA CCTCGACTCG AGCGGCAACA AGAGCGTGCT GATGGAGCGG 
CTTAAGAAGG CCATTGAAGA GGAAGGTGGG AATCCTGATG AAATTGAAGT CATCTCAGAA 
GGCAACAAGA AGATGCCTAA GAGGCCCAGC AAAGGGAAGA AACCAGAAGA CGAGGGTGTG 
GAGGACAACG GGCTCGAGGA GAATTCTGGG GATGGGCAGG AGGATGTGGA AACCAGCCTG 
GAGAACCTGC AAGACATGGA TATGATGGAC ATCAGTGTGC TGGATGAGGC TGACATTGAT 
AATGGCAGCG TGGCAGACTG TGTGGAGGAA GAAGAGGAGG CCACACTCCC TGAGGGCCTA 
GGAGACAGCA CTGAACTGGT AGAAGGCGAC TCGAAGGGGC TCCCAGAACA GCTGCAGGAG 
CATGCTATAG ATGACAAGGA AGCCATTAAC AATGTAGATA CATCATCATC TGACTTCACC 
ATCCTGCAGG AGATGGAAGA GGCATCTTTG GAGCCAGAAA ATGAGAAAAT ACTCGACATT 
TTGGGGGAAA CTTGTAAATC TGAGCCAGTA AAAGAAGAAG GTTCGGAGCT GGAGCAGCCC 
TTTGCCCAGG CCACGAGTAG CGTGGGGCCA GACAGGAAGC TGGCGGAGGA AGAGGACCTA 
TTTGAGAGCT GCGGCCACCC GGAAGAGGAA GAAGAAGAGG AGGAAGAAGA GGAGCAGGAA 
GAGGAGCAGG AGGAGGAGGG AGATTTAGCT TTGGCCAGCA GCAGCAAGTC TGAGTCTTCA 
AGCACTCGAT GTCAGTGGAG CGAGGCAGAC GCCCTGTTAG CAGTAGTGAA AAGGGAGCCG 
GCAGAGGCGC CTGGCGGAGG CACTGGGATG GACCGCGAGC CTGTAGGGCT AGAGGAGCCA 
GTTGAACAGA GTAGCACGGC TGCCCAGCTC CCGGAGACCA CCAGCCAGGA GCTGGTGCGA 
GCGCCCACGG CAGCCCCGAG CCCTGAGCCC CGAGATAGCA AAGACGACGT GAAGAAGTTT 
GCTTTTGACG CTTGTAATGA CGTCCCTGCG GCTCCTAAAG AGTCCTCAGC CAGTGAGGGC 
GCTGATCAGA AAATGAGTTC TGTGGAAGAT GACTCGGATA CAAAAAGGCT TTCCAGAGAG 
GAGAAGGGTC GCAGCAGCTG TGGTAGGAAT TTCTGGGTTA GTGGCCTTTC ATCTACGACC 
AGAGCCACAG ATCTGAAGAA CCTTTTTAGC AGATACGGGA AGGTGGTGGG AGCTAAGGTT 
GTGACGAACG CCCGGAGTCC CGGAGCTCGC TGTTACGGCT TTGTCACTAT GTCCACAGCT 
GAAGAGGCAA CAAAGTGCAT TAACCATTTG CATAAGACTG AGCTGCATGG CAAGATGATC 
TCGGTGGAAA AGGCCAAAAG TGAGCCCGCT GGCAAAAGAG TACCTGACAG GCGAGATGGC 
GACAGCAAGA AAGAGAAGAC CAGCACCAGT GACAGATCTG CAAACCTTAA GAGGGAGGAG 
AAAGGTGACA GAAAAGACGA TGCCAAGAAG ACGGATGATG GGAGCACAGA GAAGAGCAAG 
GATGCAGATG ACCAAAAGCC TGGGCCTTCA GAGCGCTCTC GAACAACCAA GTCAGGAAGC 
CGTGGCACTG AGCGTACTGT GGTGATGGAC AAGTCCAAAG GTGTGCCTGT CATCAGTGTG 
AAGACTTCTG GTTCTAAAGA GAGAGCCTCC AAAAGTCAGG ACCGAAAGTC TGTCAGCCGG 
GAGAAGCGGT CTGTTGTGTC CTTTGACAAG GTTAAAGAGT CTCGAAAGTC CAGAGACTCG 
GAGTCACGGA GGGAGCGGGA GCGGGAGCGC AGTGAGCGGG AGCAAAGGCT GCAGGCCCAG 
TGGGAGCGGG AGGAGCGAGA GCGGCTGGAG ATTGCCCGGG AGCGCCTGGC TTTCCACCGA 
CATAGGCTGG AGCGTGAACG CATGGAGAGG GAACGGTTGG AGCGGGAGCG CATGCATGTG 
GAACAGGAGC GGAGGCGTGA GCAAGAGCGC ATTCACCGAG AGCGAGAGGA GCTTCGGCGT 
CAGCAGGAAC TGCGCTATGA ACAGGAGCGG AGGCCTGCTG TGCGCAGGCC CTATGAAGTA 
GATGGCCGGC GAGATGATGC CTATTGGCCA GAAGCTAAAC GGGCTGCGCT GGATGACCGT 
TACCACTCAG ACTTCAGTCG CCAGGACCGC TTTCATGACT TCGACCACAG AGACAGAGGC 
CGCTATCCCA ACCACTCTGT GGATAGGAGA GAAGGTTCAC GGTCGATGAT GGGAGACAGA 
GAAGGACAGC ATTACCCTGA GCGCCATGGA GGACCTGAAC GCCATGGCCG GGACTCTCGA 
GATGGCTGGG GCTATGGCTC AAACAAGAGA CTGAGTGAAG GCCGGGGGCT GCCCCCTCCT 
CCCAGGCGTG ACTGGGGAGA GCATGGCCGG AGACTTGAAG ATGACCGGGC ATGGCAGGGC 
ACAGCTGATG GAGGCATGAT GGAGCGTGAT CACAAGAGAT GGCAAGGTGG TGAGAGGAGC 
ATGTCTGGCC ACTCAGGGCC CGGTCACATG ATGAATCGTG GTGGCATGTC AGGGCGAGGC 
AGTTTTGCCC CCGGAGGAGC TTCCCGTGGC CATGTGATCC CACGTGGAGG GATGCAGGCA 
GGCTTCGGCG GACAGAGCCG GGGCAGCAGA CCCAGTGATG CCCGCTTCAC CCGCCGCTAT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Safb ( XM_017596622.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017452111.1 Rattus norvegicus scaffold attachment factor B (Safb), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa62851
Clone ID Related Accession (Same CDS sequence)	XM_017596622.1
Accession Version	XM_017596622.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2829bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	scaffold attachment factor B1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGA CTCTGTCGGG TTTAGGAGAC TCGGGTGCGG CAAGCGCAGC GGCCGTGAGC 
TCCGCGGCGT CAGAGACCGG GACGCGGCGG CTCAGCGACC TGCGGGTGAT CGACCTGCGG 
GCGGAGCTGC GGAAGCGAAA CCTCGACTCG AGCGGCAACA AGAGCGTGCT GATGGAGCGG 
CTTAAGAAGG CCATTGAAGA GGAAGGTGGG AATCCTGATG AAATTGAAGT CATCTCAGAA 
GGCAACAAGA AGATGCCTAA GAGGCCCAGC AAAGGGAAGA AACCAGAAGA CGAGGGTGTG 
GAGGACAACG GGCTCGAGGA GAATTCTGGG GATGGGCAGG AGGATGTGGA AACCAGCCTG 
GAGAACCTGC AAGACATGGA TATGATGGAC ATCAGTGTGC TGGATGAGGC TGACATTGAT 
AATGGCAGCG TGGCAGACTG TGTGGAGGAA GAAGAGGAGG CCACACTCCC TGAGGGCCTA 
GGAGACAGCA CTGAACTGGT AGAAGGCGAC TCGAAGGGGC TCCCAGAACA GCTGCAGGAG 
CATGCTATAG ATGACAAGGA AGCCATTAAC AATGTAGATA CATCATCATC TGACTTCACC 
ATCCTGCAGG AGATGGAAGA GGCATCTTTG GAGCCAGAAA ATGAGAAAAT ACTCGACATT 
TTGGGGGAAA CTTGTAAATC TGAGCCAGTA AAAGAAGAAG GTTCGGAGCT GGAGCAGCCC 
TTTGCCCAGG CCACGAGTAG CGTGGGGCCA GACAGGAAGC TGGCGGAGGA AGAGGACCTA 
TTTGAGAGCT GCGGCCACCC GGAAGAGGAA GAAGAAGAGG AGGAAGAAGA GGAGCAGGAA 
GAGGAGCAGG AGGAGGAGGG AGATTTAGCT TTGGCCAGCA GCAGCAAGTC TGAGTCTTCA 
AGCACTCGAT GTCAGTGGAG CGAGGCAGAC GCCCTGTTAG CAGTAGTGAA AAGGGAGCCG 
GCAGAGGCGC CTGGCGGAGG CACTGGGATG GACCGCGAGC CTGTAGGGCT AGAGGAGCCA 
GTTGAACAGA GTAGCACGGC TGCCCAGCTC CCGGAGACCA CCAGCCAGGA GCTGGTGCGA 
GCGCCCACGG CAGCCCCGAG CCCTGAGCCC CGAGATAGCA AAGACGACGT GAAGAAGTTT 
GCTTTTGACG CTTGTAATGA CGTCCCTGCG GCTCCTAAAG AGTCCTCAGC CAGTGAGGGC 
GCTGATCAGA AAATGAGTTC TGTGGAAGAT GACTCGGATA CAAAAAGGCT TTCCAGAGAG 
GAGAAGGGTC GCAGCAGCTG TGGTAGGAAT TTCTGGGTTA GTGGCCTTTC ATCTACGACC 
AGAGCCACAG ATCTGAAGAA CCTTTTTAGC AGATACGGGA AGGTGGTGGG AGCTAAGGTT 
GTGACGAACG CCCGGAGTCC CGGAGCTCGC TGTTACGGCT TTGTCACTAT GTCCACAGCT 
GAAGAGGCAA CAAAGTGCAT TAACCATTTG CATAAGACTG AGCTGCATGG CAAGATGATC 
TCGGTGGAAA AGGCCAAAAG TGAGCCCGCT GGCAAAAGAG TACCTGACAG GCGAGATGGC 
GACAGCAAGA AAGAGAAGAC CAGCACCAGT GACAGATCTG CAAACCTTAA GAGGGAGGAG 
AAAGGTGACA GAAAAGACGA TGCCAAGAAG ACGGATGATG GGAGCACAGA GAAGAGCAAG 
GATGCAGATG ACCAAAAGCC TGGGCCTTCA GAGCGCTCTC GAACAACCAA GTCAGGAAGC 
CGTGGCACTG AGCGTACTGT GGTGATGGAC AAGTCCAAAG GTGTGCCTGT CATCAGTGTG 
AAGACTTCTG GTTCTAAAGA GAGAGCCTCC AAAAGTCAGG ACCGAAAGTC TGTCAGCCGG 
GAGAAGCGGT CTGTTGTGTC CTTTGACAAG GTTAAAGAGT CTCGAAAGTC CAGAGACTCG 
GAGTCACGGA GGGAGCGGGA GCGGGAGCGC AGTGAGCGGG AGCAAAGGCT GCAGGCCCAG 
TGGGAGCGGG AGGAGCGAGA GCGGCTGGAG ATTGCCCGGG AGCGCCTGGC TTTCCACCGA 
CATAGGCTGG AGCGTGAACG CATGGAGAGG GAACGGTTGG AGCGGGAGCG CATGCATGTG 
GAACAGGAGC GGAGGCGTGA GCAAGAGCGC ATTCACCGAG AGCGAGAGGA GCTTCGGCGT 
CAGCAGGAAC TGCGCTATGA ACAGGAGCGG AGGCCTGCTG TGCGCAGGCC CTATGAAGTA 
GATGGCCGGC GAGATGATGC CTATTGGCCA GAAGCTAAAC GGGCTGCGCT GGATGACCGT 
TACCACTCAG ACTTCAGTCG CCAGGACCGC TTTCATGACT TCGACCACAG AGACAGAGGC 
CGCTATCCCA ACCACTCTGT GGATAGGAGA GAAGGTTCAC GGTCGATGAT GGGAGACAGA 
GAAGGACAGC ATTACCCTGA GCGCCATGGA GGACCTGAAC GCCATGGCCG GGACTCTCGA 
GATGGCTGGG GCTATGGCTC AAACAAGAGA CTGAGTGAAG GCCGGGGGCT GCCCCCTCCT 
CCCAGGGGCA GGCGTGACTG GGGAGAGCAT GGCCGGAGAC TTGAAGATGA CCGGGCATGG 
CAGGGCACAG CTGATGGAGG CATGATGGAG CGTGATCACA AGAGATGGCA AGGTGGTGAG 
AGGAGCATGT CTGGCCACTC AGGGCCCGGT CACATGATGA ATCGTGGTGG CATGTCAGGG 
CGAGGCAGTT TTGCCCCCGG AGGAGCTTCC CGTGGCCATG TGATCCCACG TGGAGGGATG 
CAGGCAGGCT TCGGCGGACA GAGCCGGGGC AGCAGACCCA GTGATGCCCG CTTCACCCGC 
CGCTATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017452111.1
CDS	166..2994
Translation	MAETLSGLGDSGAASAAAVSSAASETGTRRLSDLRVIDLRAELRKRNLDSSGNKSVLMERLKKAIEEEGGNPDEIEVISEGNKKMPKRPSKGKKPEDEGVEDNGLEENSGDGQEDVETSLENLQDMDMMDISVLDEADIDNGSVADCVEEEEEATLPEGLGDSTELVEGDSKGLPEQLQEHAIDDKEAINNVDTSSSDFTILQEMEEASLEPENEKILDILGETCKSEPVKEEGSELEQPFAQATSSVGPDRKLAEEEDLFESCGHPEEEEEEEEEEEQEEEQEEEGDLALASSSKSESSSTRCQWSEADALLAVVKREPAEAPGGGTGMDREPVGLEEPVEQSSTAAQLPETTSQELVRAPTAAPSPEPRDSKDDVKKFAFDACNDVPAAPKESSASEGADQKMSSVEDDSDTKRLSREEKGRSSCGRNFWVSGLSSTTRATDLKNLFSRYGKVVGAKVVTNARSPGARCYGFVTMSTAEEATKCINHLHKTELHGKMISVEKAKSEPAGKRVPDRRDGDSKKEKTSTSDRSANLKREEKGDRKDDAKKTDDGSTEKSKDADDQKPGPSERSRTTKSGSRGTERTVVMDKSKGVPVISVKTSGSKERASKSQDRKSVSREKRSVVSFDKVKESRKSRDSESRRERERERSEREQRLQAQWEREERERLEIARERLAFHRHRLERERMERERLERERMHVEQERRREQERIHREREELRRQQELRYEQERRPAVRRPYEVDGRRDDAYWPEAKRAALDDRYHSDFSRQDRFHDFDHRDRGRYPNHSVDRREGSRSMMGDREGQHYPERHGGPERHGRDSRDGWGYGSNKRLSEGRGLPPPPRGRRDWGEHGRRLEDDRAWQGTADGGMMERDHKRWQGGERSMSGHSGPGHMMNRGGMSGRGSFAPGGASRGHVIPRGGMQAGFGGQSRGSRPSDARFTRRY

Target ORF information:

RefSeq Version	XM_017596622.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus scaffold attachment factor B (Safb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017596622.1

ORF Insert Sequence:

ATGGCGGAGA CTCTGTCGGG TTTAGGAGAC TCGGGTGCGG CAAGCGCAGC GGCCGTGAGC 
TCCGCGGCGT CAGAGACCGG GACGCGGCGG CTCAGCGACC TGCGGGTGAT CGACCTGCGG 
GCGGAGCTGC GGAAGCGAAA CCTCGACTCG AGCGGCAACA AGAGCGTGCT GATGGAGCGG 
CTTAAGAAGG CCATTGAAGA GGAAGGTGGG AATCCTGATG AAATTGAAGT CATCTCAGAA 
GGCAACAAGA AGATGCCTAA GAGGCCCAGC AAAGGGAAGA AACCAGAAGA CGAGGGTGTG 
GAGGACAACG GGCTCGAGGA GAATTCTGGG GATGGGCAGG AGGATGTGGA AACCAGCCTG 
GAGAACCTGC AAGACATGGA TATGATGGAC ATCAGTGTGC TGGATGAGGC TGACATTGAT 
AATGGCAGCG TGGCAGACTG TGTGGAGGAA GAAGAGGAGG CCACACTCCC TGAGGGCCTA 
GGAGACAGCA CTGAACTGGT AGAAGGCGAC TCGAAGGGGC TCCCAGAACA GCTGCAGGAG 
CATGCTATAG ATGACAAGGA AGCCATTAAC AATGTAGATA CATCATCATC TGACTTCACC 
ATCCTGCAGG AGATGGAAGA GGCATCTTTG GAGCCAGAAA ATGAGAAAAT ACTCGACATT 
TTGGGGGAAA CTTGTAAATC TGAGCCAGTA AAAGAAGAAG GTTCGGAGCT GGAGCAGCCC 
TTTGCCCAGG CCACGAGTAG CGTGGGGCCA GACAGGAAGC TGGCGGAGGA AGAGGACCTA 
TTTGAGAGCT GCGGCCACCC GGAAGAGGAA GAAGAAGAGG AGGAAGAAGA GGAGCAGGAA 
GAGGAGCAGG AGGAGGAGGG AGATTTAGCT TTGGCCAGCA GCAGCAAGTC TGAGTCTTCA 
AGCACTCGAT GTCAGTGGAG CGAGGCAGAC GCCCTGTTAG CAGTAGTGAA AAGGGAGCCG 
GCAGAGGCGC CTGGCGGAGG CACTGGGATG GACCGCGAGC CTGTAGGGCT AGAGGAGCCA 
GTTGAACAGA GTAGCACGGC TGCCCAGCTC CCGGAGACCA CCAGCCAGGA GCTGGTGCGA 
GCGCCCACGG CAGCCCCGAG CCCTGAGCCC CGAGATAGCA AAGACGACGT GAAGAAGTTT 
GCTTTTGACG CTTGTAATGA CGTCCCTGCG GCTCCTAAAG AGTCCTCAGC CAGTGAGGGC 
GCTGATCAGA AAATGAGTTC TGTGGAAGAT GACTCGGATA CAAAAAGGCT TTCCAGAGAG 
GAGAAGGGTC GCAGCAGCTG TGGTAGGAAT TTCTGGGTTA GTGGCCTTTC ATCTACGACC 
AGAGCCACAG ATCTGAAGAA CCTTTTTAGC AGATACGGGA AGGTGGTGGG AGCTAAGGTT 
GTGACGAACG CCCGGAGTCC CGGAGCTCGC TGTTACGGCT TTGTCACTAT GTCCACAGCT 
GAAGAGGCAA CAAAGTGCAT TAACCATTTG CATAAGACTG AGCTGCATGG CAAGATGATC 
TCGGTGGAAA AGGCCAAAAG TGAGCCCGCT GGCAAAAGAG TACCTGACAG GCGAGATGGC 
GACAGCAAGA AAGAGAAGAC CAGCACCAGT GACAGATCTG CAAACCTTAA GAGGGAGGAG 
AAAGGTGACA GAAAAGACGA TGCCAAGAAG ACGGATGATG GGAGCACAGA GAAGAGCAAG 
GATGCAGATG ACCAAAAGCC TGGGCCTTCA GAGCGCTCTC GAACAACCAA GTCAGGAAGC 
CGTGGCACTG AGCGTACTGT GGTGATGGAC AAGTCCAAAG GTGTGCCTGT CATCAGTGTG 
AAGACTTCTG GTTCTAAAGA GAGAGCCTCC AAAAGTCAGG ACCGAAAGTC TGTCAGCCGG 
GAGAAGCGGT CTGTTGTGTC CTTTGACAAG GTTAAAGAGT CTCGAAAGTC CAGAGACTCG 
GAGTCACGGA GGGAGCGGGA GCGGGAGCGC AGTGAGCGGG AGCAAAGGCT GCAGGCCCAG 
TGGGAGCGGG AGGAGCGAGA GCGGCTGGAG ATTGCCCGGG AGCGCCTGGC TTTCCACCGA 
CATAGGCTGG AGCGTGAACG CATGGAGAGG GAACGGTTGG AGCGGGAGCG CATGCATGTG 
GAACAGGAGC GGAGGCGTGA GCAAGAGCGC ATTCACCGAG AGCGAGAGGA GCTTCGGCGT 
CAGCAGGAAC TGCGCTATGA ACAGGAGCGG AGGCCTGCTG TGCGCAGGCC CTATGAAGTA 
GATGGCCGGC GAGATGATGC CTATTGGCCA GAAGCTAAAC GGGCTGCGCT GGATGACCGT 
TACCACTCAG ACTTCAGTCG CCAGGACCGC TTTCATGACT TCGACCACAG AGACAGAGGC 
CGCTATCCCA ACCACTCTGT GGATAGGAGA GAAGGTTCAC GGTCGATGAT GGGAGACAGA 
GAAGGACAGC ATTACCCTGA GCGCCATGGA GGACCTGAAC GCCATGGCCG GGACTCTCGA 
GATGGCTGGG GCTATGGCTC AAACAAGAGA CTGAGTGAAG GCCGGGGGCT GCCCCCTCCT 
CCCAGGGGCA GGCGTGACTG GGGAGAGCAT GGCCGGAGAC TTGAAGATGA CCGGGCATGG 
CAGGGCACAG CTGATGGAGG CATGATGGAG CGTGATCACA AGAGATGGCA AGGTGGTGAG 
AGGAGCATGT CTGGCCACTC AGGGCCCGGT CACATGATGA ATCGTGGTGG CATGTCAGGG 
CGAGGCAGTT TTGCCCCCGG AGGAGCTTCC CGTGGCCATG TGATCCCACG TGGAGGGATG 
CAGGCAGGCT TCGGCGGACA GAGCCGGGGC AGCAGACCCA GTGATGCCCG CTTCACCCGC 
CGCTATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanhäusser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

Heterogeneous nuclear ribonucleoprotein G regulates splice site selection by binding to CC(A/C)-rich regions in pre-mRNA.
The Journal of biological chemistry284(21)14303-15(2009 May)
Heinrich B,Zhang Z,Raitskin O,Hiller M,Benderska N,Hartmann AM,Bracco L,Elliott D,Ben-Ari S,Soreq H,Sperling J,Sperling R,Stamm S

SAFB1, an RBMX-binding protein, is a newly identified regulator of hepatic SREBP-1c gene.
BMB reports42(4)232-7(2009 Apr)
Omura Y,Nishio Y,Takemoto T,Ikeuchi C,Sekine O,Morino K,Maeno Y,Obata T,Ugi S,Maegawa H,Kimura H,Kashiwagi A

Phosphoproteomic analysis of rat liver by high capacity IMAC and LC-MS/MS.
Journal of proteome research5(1)98-104(2006 Jan)
Moser K,White FM

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Safb cDNA ORF clone, Rattus norvegicus(Norway rat)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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