Hsd17b10 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hsd17b10
Entrez Gene ID	63864
Full Name	hydroxysteroid (17-beta) dehydrogenase 10
Synonyms	Hadh2
General protein information	Preferred Names hydroxysteroid (17-beta) dehydrogenase 10 Names 3-hydroxyacyl-CoA dehydrogenase type-2 17-beta-HSD 10 17-beta-hydroxysteroid dehydrogenase 10 3-hydroxy-2-methylbutyryl-CoA dehydrogenase 3-hydroxyacyl-CoA dehydrogenase type II amyloid beta-peptide binding protein endoplasmic reticulum-associated amyloid beta-peptide-binding protein hydroxyacyl-Coenzyme A dehydrogenase, type II mitochondrial RNase P protein 2 mitochondrial ribonuclease P protein 2 short chain L-3-hydroxyacyl-CoA dehydrogenase type II HADH
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: X Map Location: Xq13
Summary	a type 10 17beta-hydroxysteroid dehydrogenase enzyme of the mitochondria [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_031682.1	NP_113870.1	3-hydroxyacyl-CoA dehydrogenase type-2

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003414	XP_003436472.1
Canis lupus familiaris (dog)	HSD17B10	XP_538051.2
Mus musculus (house mouse)	Hsd17b10	NP_058043.3
Drosophila melanogaster (fruit fly)	scu	NP_523396.1
Xenopus tropicalis (tropical clawed frog)	hsd17b10	NP_001016511.1
Rattus norvegicus (Norway rat)	Hsd17b10	NP_113870.1
Magnaporthe oryzae (rice blast fungus)	MGG_01180	XP_003714064.1
Neurospora crassa	NCU11196	XP_001728478.1
Pan troglodytes (chimpanzee)	HSD17B10	XP_003317521.1
Macaca mulatta (Rhesus monkey)	HSD17B10	XP_001087982.1
Bos taurus (cattle)	HSD17B10	NP_776759.1
Caenorhabditis elegans (roundworm)	ard-1	NP_502083.1
Homo sapiens (human)	HSD17B10	NP_004484.1
Danio rerio (zebrafish)	hsd17b10	NP_001006098.1

Gene Ontology
Bibliography

Related articles in PubMed

Novel patient missense mutations in the HSD17B10 gene affect dehydrogenase and mitochondrial tRNA modification functions of the encoded protein.
Oerum S, Roovers M, Leichsenring M, Acquaviva-Bourdain C, Beermann F, Gemperle-Britschgi C, Fouilhoux A, Korwitz-Reichelt A, Bailey HJ, Droogmans L, Oppermann U, Sass JO, Yue WW
Biochimica et biophysica acta. Molecular basis of disease1863(12)3294-3302(2017 12)

The MRPP1/MRPP2 complex is a tRNA-maturation platform in human mitochondria.
Reinhard L, Sridhara S, Hällberg BM
Nucleic acids research45(21)12469-12480(2017 Dec)

Molecular insights into HSD10 disease: impact of SDR5C1 mutations on the human mitochondrial RNase P complex.
Vilardo E, Rossmanith W
Nucleic acids research43(10)5112-9(2015 May)

Overexpression of 17β-hydroxysteroid dehydrogenase type 10 increases pheochromocytoma cell growth and resistance to cell death.
Carlson EA, Marquez RT, Du F, Wang Y, Xu L, Yan SS
BMC cancer15166(2015 Mar)

Initial steps in RNA processing and ribosome assembly occur at mitochondrial DNA nucleoids.
Bogenhagen DF, Martin DW, Koller A
Cell metabolism19(4)618-29(2014 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Hsd17b10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hsd17b10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12442	NM_031682.1 Latest version!	Rattus norvegicus hydroxysteroid (17-beta) dehydrogenase 10 (Hsd17b10), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hsd17b10 ( NM_031682.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_113870.1 Rattus norvegicus hydroxysteroid (17-beta) dehydrogenase 10 (Hsd17b10), mRNA.

Price & Availability↑

CloneID	ORa12442
Clone ID Related Accession (Same CDS sequence)	NM_031682.1
Accession Version	NM_031682.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 786bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577116800000
Organism	Rattus norvegicus(Norway rat)
Product	3-hydroxyacyl-CoA dehydrogenase type-2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF069770.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF069770.1, BC158587.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGCTGCGG CGTGTCGGAG CGTGAAGGGC CTGGTGGCAG TAATAACCGG AGGAGCCTCG 
GGCCTTGGCC TGTCTACAGC CAAAAGACTG GTGGGACAAG GAGCCACAGC TGTACTTCTG 
GACGTCCCTA ACTCAGAGGG TGAGACCGAA GCCAAGAAGT TAGGAGGAAA CTGCATATTT 
GCCCCAGCAA ATGTGACCTC TGAGAAGGAG GTACAAGCAG CTTTGACTCT AGCAAAAGAA 
AAGTTTGGCC GTATAGATGT GGCTGTCAAC TGTGCAGGCA TTGCAGTGGC CATTAAGACA 
TACCACGAAA AGAAGAACCA GGTCCACACC TTGGAGGACT TCCAGCGGGT TATCAATGTG 
AATCTCATAG GCACTTTCAA TGTGATCCGC CTGGTTGCTG GTGTGATGGG CCAGAATGAA 
CCAGACCAGG GAGGTCAACG TGGAGTTATC ATTAACACTG CCAGTGTGGC TGCTTTTGAG 
GGCCAGGTTG GACAAGCTGC ATACTCTGCA TCCAAAGGGG GCATAGTGGG CATGACACTG 
CCCATTGCTC GAGATCTGGC TCCTATAGGC ATCCGTGTGG TAACAATTGC TCCAGGTCTG 
TTTGCCACCC CACTGCTTAC CACTCTGCCA GACAAAGTGC GCAACTTCTT GGCCAGCCAG 
GTACCCTTCC CCAGCCGACT AGGTGACCCC GCGGAATATG CTCATCTGGT ACAGATGGTA 
ATCGAGAACC CATTCTTGAA TGGAGAGGTC ATCCGGCTGG ATGGGGCCAT TCGAATGCAG 
CCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_113870.1
CDS	13..798
Translation	MAAACRSVKGLVAVITGGASGLGLSTAKRLVGQGATAVLLDVPNSEGETEAKKLGGNCIFAPANVTSEKEVQAALTLAKEKFGRIDVAVNCAGIAVAIKTYHEKKNQVHTLEDFQRVINVNLIGTFNVIRLVAGVMGQNEPDQGGQRGVIINTASVAAFEGQVGQAAYSASKGGIVGMTLPIARDLAPIGIRVVTIAPGLFATPLLTTLPDKVRNFLASQVPFPSRLGDPAEYAHLVQMVIENPFLNGEVIRLDGAIRMQP

Target ORF information:

RefSeq Version	NM_031682.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus hydroxysteroid (17-beta) dehydrogenase 10 (Hsd17b10), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031682.1

ORF Insert Sequence:

ATGGCTGCGG CGTGTCGGAG CGTGAAGGGC CTGGTGGCAG TAATAACCGG AGGAGCCTCG 
GGCCTTGGCC TGTCTACAGC CAAAAGACTG GTGGGACAAG GAGCCACAGC TGTACTTCTG 
GACGTCCCTA ACTCAGAGGG TGAGACCGAA GCCAAGAAGT TAGGAGGAAA CTGCATATTT 
GCCCCAGCAA ATGTGACCTC TGAGAAGGAG GTACAAGCAG CTTTGACTCT AGCAAAAGAA 
AAGTTTGGCC GTATAGATGT GGCTGTCAAC TGTGCAGGCA TTGCAGTGGC CATTAAGACA 
TACCACGAAA AGAAGAACCA GGTCCACACC TTGGAGGACT TCCAGCGGGT TATCAATGTG 
AATCTCATAG GCACTTTCAA TGTGATCCGC CTGGTTGCTG GTGTGATGGG CCAGAATGAA 
CCAGACCAGG GAGGTCAACG TGGAGTTATC ATTAACACTG CCAGTGTGGC TGCTTTTGAG 
GGCCAGGTTG GACAAGCTGC ATACTCTGCA TCCAAAGGGG GCATAGTGGG CATGACACTG 
CCCATTGCTC GAGATCTGGC TCCTATAGGC ATCCGTGTGG TAACAATTGC TCCAGGTCTG 
TTTGCCACCC CACTGCTTAC CACTCTGCCA GACAAAGTGC GCAACTTCTT GGCCAGCCAG 
GTACCCTTCC CCAGCCGACT AGGTGACCCC GCGGAATATG CTCATCTGGT ACAGATGGTA 
ATCGAGAACC CATTCTTGAA TGGAGAGGTC ATCCGGCTGG ATGGGGCCAT TCGAATGCAG 
CCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Novel patient missense mutations in the HSD17B10 gene affect dehydrogenase and mitochondrial tRNA modification functions of the encoded protein.
Biochimica et biophysica acta. Molecular basis of disease1863(12)3294-3302(2017 12)
Oerum S,Roovers M,Leichsenring M,Acquaviva-Bourdain C,Beermann F,Gemperle-Britschgi C,Fouilhoux A,Korwitz-Reichelt A,Bailey HJ,Droogmans L,Oppermann U,Sass JO,Yue WW

The MRPP1/MRPP2 complex is a tRNA-maturation platform in human mitochondria.
Nucleic acids research45(21)12469-12480(2017 Dec)
Reinhard L,Sridhara S,Hällberg BM

Molecular insights into HSD10 disease: impact of SDR5C1 mutations on the human mitochondrial RNase P complex.
Nucleic acids research43(10)5112-9(2015 May)
Vilardo E,Rossmanith W

Overexpression of 17β-hydroxysteroid dehydrogenase type 10 increases pheochromocytoma cell growth and resistance to cell death.
BMC cancer15166(2015 Mar)
Carlson EA,Marquez RT,Du F,Wang Y,Xu L,Yan SS

Initial steps in RNA processing and ribosome assembly occur at mitochondrial DNA nucleoids.
Cell metabolism19(4)618-29(2014 Apr)
Bogenhagen DF,Martin DW,Koller A