Clcn4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Clcn4
Entrez Gene ID	60586
Full Name	chloride voltage-gated channel 4
Synonyms	Clcn4-2
General protein information	Preferred Names chloride voltage-gated channel 4 Names H(+)/Cl(-) exchange transporter 4 chloride channel 4 chloride channel 4-2 chloride channel protein 4 chloride channel, voltage-sensitive 4 chloride transporter ClC-4 clC-4 putative chloride channel (similar to Mm Clcn4-2) putative chloride channel 4-2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: X Map Location: Xq13
Summary	may act as a chloride channel [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006256828.3	XP_006256890.1	H(+)/Cl(-) exchange transporter 4 isoform X1
NM_022198.2	NP_071534.2	H(+)/Cl(-) exchange transporter 4

Pathways from BioSystems

Homologies

Bos taurus (cattle)	CLCN4	NP_001026927.1
Gallus gallus (chicken)	CLCN4	XP_425575.4
Canis lupus familiaris (dog)	CLCN4	XP_848379.1
Magnaporthe oryzae (rice blast fungus)	MGG_00468	XP_003718596.1
Rattus norvegicus (Norway rat)	Clcn4	NP_071534.2
Xenopus tropicalis (tropical clawed frog)	clcn4	NP_001120070.1
Mus musculus (house mouse)	Clcn4-2	NP_035464.3
Danio rerio (zebrafish)	clcn4	NP_001070786.1
Neurospora crassa	NCU06444	XP_957335.2
Homo sapiens (human)	CLCN4	NP_001821.2

Gene Ontology
Bibliography

Related articles in PubMed

X-exome sequencing of 405 unresolved families identifies seven novel intellectual disability genes.
Hu H, Haas SA, Chelly J, Van Esch H, Raynaud M, de Brouwer AP, Weinert S, Froyen G, Frints SG, Laumonnier F, Zemojtel T, Love MI, Richard H, Emde AK, Bienek M, Jensen C, Hambrock M, Fischer U, Langnick C, Feldkamp M, Wissink-Lindhout W, Lebrun N, Castelnau L, Rucci J, Montjean R, Dorseuil O, Billuart P, Stuhlmann T, Shaw M, Corbett MA, Gardner A, Willis-Owen S, Tan C, Friend KL, Belet S, van Roozendaal KE, Jimenez-Pocquet M, Moizard MP, Ronce N, Sun R, O'Keeffe S, Chenna R, van Bömmel A, Göke J, Hackett A, Field M, Christie L, Boyle J, Haan E, Nelson J, Turner G, Baynam G, Gillessen-Kaesbach G, Müller U, Steinberger D, Budny B, Badura-Stronka M, Latos-Bieleńska A, Ousager LB, Wieacker P, Rodríguez Criado G, Bondeson ML, Annerén G, Dufke A, Cohen M, Van Maldergem L, Vincent-Delorme C, Echenne B, Simon-Bouy B, Kleefstra T, Willemsen M, Fryns JP, Devriendt K, Ullmann R, Vingron M, Wrogemann K, Wienker TF, Tzschach A, van Bokhoven H, Gecz J, Jentsch TJ, Chen W, Ropers HH, Kalscheuer VM
Molecular psychiatry21(1)133-48(2016 Jan)

Exome sequencing reveals new causal mutations in children with epileptic encephalopathies.
Veeramah KR, Johnstone L, Karafet TM, Wolf D, Sprissler R, Salogiannis J, Barth-Maron A, Greenberg ME, Stuhlmann T, Weinert S, Jentsch TJ, Pazzi M, Restifo LL, Talwar D, Erickson RP, Hammer MF
Epilepsia54(7)1270-81(2013 Jul)

The human ClC-4 protein, a member of the CLC chloride channel/transporter family, is localized to the endoplasmic reticulum by its N-terminus.
Okkenhaug H, Weylandt KH, Carmena D, Wells DJ, Higgins CF, Sardini A
FASEB journal : official publication of the Federation of American Societies for Experimental Biology20(13)2390-2(2006 Nov)

Identification of an acid-activated Cl(-) channel from human skeletal muscles.
Kawasaki M, Fukuma T, Yamauchi K, Sakamoto H, Marumo F, Sasaki S
The American journal of physiology277(5)C948-54(1999 11)

Properties of voltage-gated chloride channels of the ClC gene family.
Jentsch TJ, Günther W, Pusch M, Schwappach B
The Journal of physiology48219S-25S(1995 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Clcn4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Clcn4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa05416	NM_022198.2 Latest version!	Rattus norvegicus chloride voltage-gated channel 4 (Clcn4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ORa39662	XM_006256828.3 Latest version!	Rattus norvegicus chloride voltage-gated channel 4 (Clcn4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Clcn4 ( NM_022198.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_071534.2 Rattus norvegicus chloride voltage-gated channel 4 (Clcn4), mRNA.

Price & Availability↑

CloneID	ORa05416
Clone ID Related Accession (Same CDS sequence)	NM_022198.2
Accession Version	NM_022198.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2265bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569168000000
Organism	Rattus norvegicus(Norway rat)
Product	H(+)/Cl(-) exchange transporter 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC092209.1. On Sep 29, 2005 this sequence version replaced NM_022198.1. ##Evidence-Data-START## Transcript exon combination :: BC092209.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGAGTGCCT CTGGGAACCT AATGGACTTC CTTGATGAGC CCTTCCCTGA TGTGGGGACC 
TATGAAGACT TCCACACCAT AGACTGGCTG AGGGAAAAGT CCCGGGACAC TGACAGACAT 
AGAAAGATAA CCAGCAAAAG TAAAGAGTCT ATTTGGGAGT TCATCAAGAG TCTGCTGGAC 
GCATGGTCAG GATGGGTGGT GATGCTACTT ATTGGTCTGC TAGCAGGTAC CCTGGCTGGA 
GTTATCGATC TCGCTGTTGA CTGGATGACA GACCTCAAGG AGGGCGTCTG TCTGTCTGCA 
TTCTGGTACA GCCATGAGCA ATGCTGCTGG ACCTCCAATG AGACCACTTT TGAGGACAGG 
GACAAGTGTC CCCTGTGGCA GAAGTGGTCA GAGCTTCTTC TGAGCCAGTC AGAGGGTGCT 
AGCGCTTACA TTCTGAATTA CTTAATGTAC ATTCTATGGG CATTGCTGTT TGCATTTCTG 
GCTGTCTCCC TGGTACGTGT GTTTGCACCA TATGCCTGTG GCTCTGGCAT ACCTGAGATA 
AAGACTATTT TGAGTGGCTT TATCATCAGG GGCTACTTGG GGAAATGGAC TCTTCTAATC 
AAGACTGTCA CTCTCGTGCT GGTTGTATCC TCTGGCCTGA GTCTTGGGAA AGAGGGGCCA 
CTGGTGCATG TGGCATGTTG CTGTGGCAAC TTCTTTAGCA GCCTTTTCTC CAAGTATAGC 
AAGAATGAAG GCAAGAGGCG TGAGGTGCTT TCCGCTGCAG CTGCTGCTGG TGTCTCTGTG 
GCCTTTGGTG CTCCCATAGG AGGTGTACTC TTCAGTCTAG AGGAGGTCAG TTACTACTTT 
CCCTTGAAAA CCTTGTGGAG GTCATTTTTT GCAGCCCTGG TGGCTGCCTT CACACTGCGC 
TCCATCAACC CCTTTGGAAA TAGCCGCCTG GTTCTCTTTT ATGTGGAGTA TCATACACCT 
TGGTACATGG CTGAACTCTT CCCTTTCATC CTGCTTGGAG TCTTTGGGGG TTTGTGGGGA 
ACCGTCTTCA CACGCTGCAA CATTGCCTGG TGCAGGAGGC GTAAAACCAC CAGGCTGGGG 
AAGTATCCAG TGTTGGAGGT CATTGTGGTG ACAGCCATCA CTGCCATCAT TGCTTATCCC 
AATCCCTACA CTCGCCAGAG CACCAGTGAG CTCATATCTG AGCTATTCAA TGATTGTGGG 
GCTCTTGAGT CTTCTCAGCT CTGTGACTAC ATCAATGACC CCAACATGAC TCGGCCTGTG 
GATGACATTC CGGACCGGCC AGCTGGGGTT GGAGTTTACA CAGCCATGTG GCAGCTGGCC 
TTGGCACTGA TCTTCAAAAT AGTCATTACT ATATTTACCT TTGGCATGAA GATTCCCTCA 
GGTCTCTTCA TCCCCAGTAT GGCTGTAGGA GCCATGGCAG GCCGGATGGT GGGAATTGGT 
GTGGAGCAGC TGGCCTACCA TCATCATGAC TGGATCATCT TCAGGAACTG GTGCAGGCCT 
GGAGCGGACT GTGTCACACC AGGGCTTTAT GCGATGGTGG GAGCTGCAGC CTGTCTAGGT 
GGAGTGACTA GGATGACAGT GTCTCTGGTA GTCATCATGT TTGAACTGAC TGGAGGTCTG 
GAATATATTG TACCCCTGAT GGCAGCTGCT GTTACCAGCA AGTGGGTGGC TGATGCCTTT 
GGGAAAGAAG GGATTTATGA AGCCCACATC CATCTGAATG GGTACCCATT TCTTGATGTG 
AAGGATGAGT TCACTCACCG TACCCTAGCC ACTGATGTGA TGCGGCCCCG CAGGGGAGAA 
CCACCTTTGT CAGTACTAAC CCAGGACAGC ATGACTGTGG AGGATGTGGA GACTCTCATA 
AAAGAGACAG ACTACAATGG CTTTCCTGTG GTGGTCTCGA GAGATTCGGA GCGCCTCATA 
GGCTTTGCCC AGAGGCGGGA GCTAATCTTG GCTATAAAAA ATGCCAGACA GAGGCAAGAG 
GGCATCGTGA GCAATTCCAT CATGTACTTC ACAGAGGAGC CCCCTGAGCT GCCTGCCAAC 
AGCCCACATC CACTGAAGCT GAGGCGCATT CTGAACCTGA GCCCTTTTAC TGTCACAGAC 
CACACCCCCA TGGAGACAGT GGTGGACATT TTCCGGAAAC TGGGGCTCCG ACAATGCCTG 
GTGACACGGA GTGGGAGGCT TCTTGGGATC ATCACAAAAA AAGACGTTCT GAGACACATG 
GCGCAGATGG CAAACCAGGA CCCTGAATCC ATCATGTTTA ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071534.2
CDS	272..2536
Translation	MSASGNLMDFLDEPFPDVGTYEDFHTIDWLREKSRDTDRHRKITSKSKESIWEFIKSLLDAWSGWVVMLLIGLLAGTLAGVIDLAVDWMTDLKEGVCLSAFWYSHEQCCWTSNETTFEDRDKCPLWQKWSELLLSQSEGASAYILNYLMYILWALLFAFLAVSLVRVFAPYACGSGIPEIKTILSGFIIRGYLGKWTLLIKTVTLVLVVSSGLSLGKEGPLVHVACCCGNFFSSLFSKYSKNEGKRREVLSAAAAAGVSVAFGAPIGGVLFSLEEVSYYFPLKTLWRSFFAALVAAFTLRSINPFGNSRLVLFYVEYHTPWYMAELFPFILLGVFGGLWGTVFTRCNIAWCRRRKTTRLGKYPVLEVIVVTAITAIIAYPNPYTRQSTSELISELFNDCGALESSQLCDYINDPNMTRPVDDIPDRPAGVGVYTAMWQLALALIFKIVITIFTFGMKIPSGLFIPSMAVGAMAGRMVGIGVEQLAYHHHDWIIFRNWCRPGADCVTPGLYAMVGAAACLGGVTRMTVSLVVIMFELTGGLEYIVPLMAAAVTSKWVADAFGKEGIYEAHIHLNGYPFLDVKDEFTHRTLATDVMRPRRGEPPLSVLTQDSMTVEDVETLIKETDYNGFPVVVSRDSERLIGFAQRRELILAIKNARQRQEGIVSNSIMYFTEEPPELPANSPHPLKLRRILNLSPFTVTDHTPMETVVDIFRKLGLRQCLVTRSGRLLGIITKKDVLRHMAQMANQDPESIMFN

Target ORF information:

RefSeq Version	NM_022198.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus chloride voltage-gated channel 4 (Clcn4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022198.2

ORF Insert Sequence:

ATGAGTGCCT CTGGGAACCT AATGGACTTC CTTGATGAGC CCTTCCCTGA TGTGGGGACC 
TATGAAGACT TCCACACCAT AGACTGGCTG AGGGAAAAGT CCCGGGACAC TGACAGACAT 
AGAAAGATAA CCAGCAAAAG TAAAGAGTCT ATTTGGGAGT TCATCAAGAG TCTGCTGGAC 
GCATGGTCAG GATGGGTGGT GATGCTACTT ATTGGTCTGC TAGCAGGTAC CCTGGCTGGA 
GTTATCGATC TCGCTGTTGA CTGGATGACA GACCTCAAGG AGGGCGTCTG TCTGTCTGCA 
TTCTGGTACA GCCATGAGCA ATGCTGCTGG ACCTCCAATG AGACCACTTT TGAGGACAGG 
GACAAGTGTC CCCTGTGGCA GAAGTGGTCA GAGCTTCTTC TGAGCCAGTC AGAGGGTGCT 
AGCGCTTACA TTCTGAATTA CTTAATGTAC ATTCTATGGG CATTGCTGTT TGCATTTCTG 
GCTGTCTCCC TGGTACGTGT GTTTGCACCA TATGCCTGTG GCTCTGGCAT ACCTGAGATA 
AAGACTATTT TGAGTGGCTT TATCATCAGG GGCTACTTGG GGAAATGGAC TCTTCTAATC 
AAGACTGTCA CTCTCGTGCT GGTTGTATCC TCTGGCCTGA GTCTTGGGAA AGAGGGGCCA 
CTGGTGCATG TGGCATGTTG CTGTGGCAAC TTCTTTAGCA GCCTTTTCTC CAAGTATAGC 
AAGAATGAAG GCAAGAGGCG TGAGGTGCTT TCCGCTGCAG CTGCTGCTGG TGTCTCTGTG 
GCCTTTGGTG CTCCCATAGG AGGTGTACTC TTCAGTCTAG AGGAGGTCAG TTACTACTTT 
CCCTTGAAAA CCTTGTGGAG GTCATTTTTT GCAGCCCTGG TGGCTGCCTT CACACTGCGC 
TCCATCAACC CCTTTGGAAA TAGCCGCCTG GTTCTCTTTT ATGTGGAGTA TCATACACCT 
TGGTACATGG CTGAACTCTT CCCTTTCATC CTGCTTGGAG TCTTTGGGGG TTTGTGGGGA 
ACCGTCTTCA CACGCTGCAA CATTGCCTGG TGCAGGAGGC GTAAAACCAC CAGGCTGGGG 
AAGTATCCAG TGTTGGAGGT CATTGTGGTG ACAGCCATCA CTGCCATCAT TGCTTATCCC 
AATCCCTACA CTCGCCAGAG CACCAGTGAG CTCATATCTG AGCTATTCAA TGATTGTGGG 
GCTCTTGAGT CTTCTCAGCT CTGTGACTAC ATCAATGACC CCAACATGAC TCGGCCTGTG 
GATGACATTC CGGACCGGCC AGCTGGGGTT GGAGTTTACA CAGCCATGTG GCAGCTGGCC 
TTGGCACTGA TCTTCAAAAT AGTCATTACT ATATTTACCT TTGGCATGAA GATTCCCTCA 
GGTCTCTTCA TCCCCAGTAT GGCTGTAGGA GCCATGGCAG GCCGGATGGT GGGAATTGGT 
GTGGAGCAGC TGGCCTACCA TCATCATGAC TGGATCATCT TCAGGAACTG GTGCAGGCCT 
GGAGCGGACT GTGTCACACC AGGGCTTTAT GCGATGGTGG GAGCTGCAGC CTGTCTAGGT 
GGAGTGACTA GGATGACAGT GTCTCTGGTA GTCATCATGT TTGAACTGAC TGGAGGTCTG 
GAATATATTG TACCCCTGAT GGCAGCTGCT GTTACCAGCA AGTGGGTGGC TGATGCCTTT 
GGGAAAGAAG GGATTTATGA AGCCCACATC CATCTGAATG GGTACCCATT TCTTGATGTG 
AAGGATGAGT TCACTCACCG TACCCTAGCC ACTGATGTGA TGCGGCCCCG CAGGGGAGAA 
CCACCTTTGT CAGTACTAAC CCAGGACAGC ATGACTGTGG AGGATGTGGA GACTCTCATA 
AAAGAGACAG ACTACAATGG CTTTCCTGTG GTGGTCTCGA GAGATTCGGA GCGCCTCATA 
GGCTTTGCCC AGAGGCGGGA GCTAATCTTG GCTATAAAAA ATGCCAGACA GAGGCAAGAG 
GGCATCGTGA GCAATTCCAT CATGTACTTC ACAGAGGAGC CCCCTGAGCT GCCTGCCAAC 
AGCCCACATC CACTGAAGCT GAGGCGCATT CTGAACCTGA GCCCTTTTAC TGTCACAGAC 
CACACCCCCA TGGAGACAGT GGTGGACATT TTCCGGAAAC TGGGGCTCCG ACAATGCCTG 
GTGACACGGA GTGGGAGGCT TCTTGGGATC ATCACAAAAA AAGACGTTCT GAGACACATG 
GCGCAGATGG CAAACCAGGA CCCTGAATCC ATCATGTTTA ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Clcn4 ( XM_006256828.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006256890.1 Rattus norvegicus chloride voltage-gated channel 4 (Clcn4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa39662
Clone ID Related Accession (Same CDS sequence)	XM_006256828.3
Accession Version	XM_006256828.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2325bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	H(+)/Cl(-) exchange transporter 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006256828.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCACT GCAGGTGGAA TTCTCTTCCA GGAGTAACTA GCACGGTCAG TGCAGGAGCC 
ATGAGTGCCT CTGGGAACCT AATGGACTTC CTTGATGAGC CCTTCCCTGA TGTGGGGACC 
TATGAAGACT TCCACACCAT AGACTGGCTG AGGGAAAAGT CCCGGGACAC TGACAGACAT 
AGAAAGATAA CCAGCAAAAG TAAAGAGTCT ATTTGGGAGT TCATCAAGAG TCTGCTGGAC 
GCATGGTCAG GATGGGTGGT GATGCTACTT ATTGGTCTGC TAGCAGGTAC CCTGGCTGGA 
GTTATCGATC TCGCTGTTGA CTGGATGACA GACCTCAAGG AGGGCGTCTG TCTGTCTGCA 
TTCTGGTACA GCCATGAGCA ATGCTGCTGG ACCTCCAATG AGACCACTTT TGAGGACAGG 
GACAAGTGTC CCCTGTGGCA GAAGTGGTCA GAGCTTCTTC TGAGCCAGTC AGAGGGTGCT 
AGCGCTTACA TTCTGAATTA CTTAATGTAC ATTCTATGGG CATTGCTGTT TGCATTTCTG 
GCTGTCTCCC TGGTACGTGT GTTTGCACCA TATGCCTGTG GCTCTGGCAT ACCTGAGATA 
AAGACTATTT TGAGTGGCTT TATCATCAGG GGCTACTTGG GGAAATGGAC TCTTCTAATC 
AAGACTGTCA CTCTCGTGCT GGTTGTATCC TCTGGCCTGA GTCTTGGGAA AGAGGGGCCA 
CTGGTGCATG TGGCATGTTG CTGTGGCAAC TTCTTTAGCA GCCTTTTCTC CAAGTATAGC 
AAGAATGAAG GCAAGAGGCG TGAGGTGCTT TCCGCTGCAG CTGCTGCTGG TGTCTCTGTG 
GCCTTTGGTG CTCCCATAGG AGGTGTACTC TTCAGTCTAG AGGAGGTCAG TTACTACTTT 
CCCTTGAAAA CCTTGTGGAG GTCATTTTTT GCAGCCCTGG TGGCTGCCTT CACACTGCGC 
TCCATCAACC CCTTTGGAAA TAGCCGCCTG GTTCTCTTTT ATGTGGAGTA TCATACACCT 
TGGTACATGG CTGAACTCTT CCCTTTCATC CTGCTTGGAG TCTTTGGGGG TTTGTGGGGA 
ACCGTCTTCA CACGCTGCAA CATTGCCTGG TGCAGGAGGC GTAAAACCAC CAGGCTGGGG 
AAGTATCCAG TGTTGGAGGT CATTGTGGTG ACAGCCATCA CTGCCATCAT TGCTTATCCC 
AATCCCTACA CTCGCCAGAG CACCAGTGAG CTCATATCTG AGCTATTCAA TGATTGTGGG 
GCTCTTGAGT CTTCTCAGCT CTGTGACTAC ATCAATGACC CCAACATGAC TCGGCCTGTG 
GATGACATTC CGGACCGGCC AGCTGGGGTT GGAGTTTACA CAGCCATGTG GCAGCTGGCC 
TTGGCACTGA TCTTCAAAAT AGTCATTACT ATATTTACCT TTGGCATGAA GATTCCCTCA 
GGTCTCTTCA TCCCCAGTAT GGCTGTAGGA GCCATGGCAG GCCGGATGGT GGGAATTGGT 
GTGGAGCAGC TGGCCTACCA TCATCATGAC TGGATCATCT TCAGGAACTG GTGCAGGCCT 
GGAGCGGACT GTGTCACACC AGGGCTTTAT GCGATGGTGG GAGCTGCAGC CTGTCTAGGT 
GGAGTGACTA GGATGACAGT GTCTCTGGTA GTCATCATGT TTGAACTGAC TGGAGGTCTG 
GAATATATTG TACCCCTGAT GGCAGCTGCT GTTACCAGCA AGTGGGTGGC TGATGCCTTT 
GGGAAAGAAG GGATTTATGA AGCCCACATC CATCTGAATG GGTACCCATT TCTTGATGTG 
AAGGATGAGT TCACTCACCG TACCCTAGCC ACTGATGTGA TGCGGCCCCG CAGGGGAGAA 
CCACCTTTGT CAGTACTAAC CCAGGACAGC ATGACTGTGG AGGATGTGGA GACTCTCATA 
AAAGAGACAG ACTACAATGG CTTTCCTGTG GTGGTCTCGA GAGATTCGGA GCGCCTCATA 
GGCTTTGCCC AGAGGCGGGA GCTAATCTTG GCTATAAAAA ATGCCAGACA GAGGCAAGAG 
GGCATCGTGA GCAATTCCAT CATGTACTTC ACAGAGGAGC CCCCTGAGCT GCCTGCCAAC 
AGCCCACATC CACTGAAGCT GAGGCGCATT CTGAACCTGA GCCCTTTTAC TGTCACAGAC 
CACACCCCCA TGGAGACAGT GGTGGACATT TTCCGGAAAC TGGGGCTCCG ACAATGCCTG 
GTGACACGGA GTGGGAGGCT TCTTGGGATC ATCACAAAAA AAGACGTTCT GAGACACATG 
GCGCAGATGG CAAACCAGGA CCCTGAATCC ATCATGTTTA ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006256890.1
CDS	453..2777
Translation	MRHCRWNSLPGVTSTVSAGAMSASGNLMDFLDEPFPDVGTYEDFHTIDWLREKSRDTDRHRKITSKSKESIWEFIKSLLDAWSGWVVMLLIGLLAGTLAGVIDLAVDWMTDLKEGVCLSAFWYSHEQCCWTSNETTFEDRDKCPLWQKWSELLLSQSEGASAYILNYLMYILWALLFAFLAVSLVRVFAPYACGSGIPEIKTILSGFIIRGYLGKWTLLIKTVTLVLVVSSGLSLGKEGPLVHVACCCGNFFSSLFSKYSKNEGKRREVLSAAAAAGVSVAFGAPIGGVLFSLEEVSYYFPLKTLWRSFFAALVAAFTLRSINPFGNSRLVLFYVEYHTPWYMAELFPFILLGVFGGLWGTVFTRCNIAWCRRRKTTRLGKYPVLEVIVVTAITAIIAYPNPYTRQSTSELISELFNDCGALESSQLCDYINDPNMTRPVDDIPDRPAGVGVYTAMWQLALALIFKIVITIFTFGMKIPSGLFIPSMAVGAMAGRMVGIGVEQLAYHHHDWIIFRNWCRPGADCVTPGLYAMVGAAACLGGVTRMTVSLVVIMFELTGGLEYIVPLMAAAVTSKWVADAFGKEGIYEAHIHLNGYPFLDVKDEFTHRTLATDVMRPRRGEPPLSVLTQDSMTVEDVETLIKETDYNGFPVVVSRDSERLIGFAQRRELILAIKNARQRQEGIVSNSIMYFTEEPPELPANSPHPLKLRRILNLSPFTVTDHTPMETVVDIFRKLGLRQCLVTRSGRLLGIITKKDVLRHMAQMANQDPESIMFN

Target ORF information:

RefSeq Version	XM_006256828.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus chloride voltage-gated channel 4 (Clcn4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006256828.3

ORF Insert Sequence:

ATGCGGCACT GCAGGTGGAA TTCTCTTCCA GGAGTAACTA GCACGGTCAG TGCAGGAGCC 
ATGAGTGCCT CTGGGAACCT AATGGACTTC CTTGATGAGC CCTTCCCTGA TGTGGGGACC 
TATGAAGACT TCCACACCAT AGACTGGCTG AGGGAAAAGT CCCGGGACAC TGACAGACAT 
AGAAAGATAA CCAGCAAAAG TAAAGAGTCT ATTTGGGAGT TCATCAAGAG TCTGCTGGAC 
GCATGGTCAG GATGGGTGGT GATGCTACTT ATTGGTCTGC TAGCAGGTAC CCTGGCTGGA 
GTTATCGATC TCGCTGTTGA CTGGATGACA GACCTCAAGG AGGGCGTCTG TCTGTCTGCA 
TTCTGGTACA GCCATGAGCA ATGCTGCTGG ACCTCCAATG AGACCACTTT TGAGGACAGG 
GACAAGTGTC CCCTGTGGCA GAAGTGGTCA GAGCTTCTTC TGAGCCAGTC AGAGGGTGCT 
AGCGCTTACA TTCTGAATTA CTTAATGTAC ATTCTATGGG CATTGCTGTT TGCATTTCTG 
GCTGTCTCCC TGGTACGTGT GTTTGCACCA TATGCCTGTG GCTCTGGCAT ACCTGAGATA 
AAGACTATTT TGAGTGGCTT TATCATCAGG GGCTACTTGG GGAAATGGAC TCTTCTAATC 
AAGACTGTCA CTCTCGTGCT GGTTGTATCC TCTGGCCTGA GTCTTGGGAA AGAGGGGCCA 
CTGGTGCATG TGGCATGTTG CTGTGGCAAC TTCTTTAGCA GCCTTTTCTC CAAGTATAGC 
AAGAATGAAG GCAAGAGGCG TGAGGTGCTT TCCGCTGCAG CTGCTGCTGG TGTCTCTGTG 
GCCTTTGGTG CTCCCATAGG AGGTGTACTC TTCAGTCTAG AGGAGGTCAG TTACTACTTT 
CCCTTGAAAA CCTTGTGGAG GTCATTTTTT GCAGCCCTGG TGGCTGCCTT CACACTGCGC 
TCCATCAACC CCTTTGGAAA TAGCCGCCTG GTTCTCTTTT ATGTGGAGTA TCATACACCT 
TGGTACATGG CTGAACTCTT CCCTTTCATC CTGCTTGGAG TCTTTGGGGG TTTGTGGGGA 
ACCGTCTTCA CACGCTGCAA CATTGCCTGG TGCAGGAGGC GTAAAACCAC CAGGCTGGGG 
AAGTATCCAG TGTTGGAGGT CATTGTGGTG ACAGCCATCA CTGCCATCAT TGCTTATCCC 
AATCCCTACA CTCGCCAGAG CACCAGTGAG CTCATATCTG AGCTATTCAA TGATTGTGGG 
GCTCTTGAGT CTTCTCAGCT CTGTGACTAC ATCAATGACC CCAACATGAC TCGGCCTGTG 
GATGACATTC CGGACCGGCC AGCTGGGGTT GGAGTTTACA CAGCCATGTG GCAGCTGGCC 
TTGGCACTGA TCTTCAAAAT AGTCATTACT ATATTTACCT TTGGCATGAA GATTCCCTCA 
GGTCTCTTCA TCCCCAGTAT GGCTGTAGGA GCCATGGCAG GCCGGATGGT GGGAATTGGT 
GTGGAGCAGC TGGCCTACCA TCATCATGAC TGGATCATCT TCAGGAACTG GTGCAGGCCT 
GGAGCGGACT GTGTCACACC AGGGCTTTAT GCGATGGTGG GAGCTGCAGC CTGTCTAGGT 
GGAGTGACTA GGATGACAGT GTCTCTGGTA GTCATCATGT TTGAACTGAC TGGAGGTCTG 
GAATATATTG TACCCCTGAT GGCAGCTGCT GTTACCAGCA AGTGGGTGGC TGATGCCTTT 
GGGAAAGAAG GGATTTATGA AGCCCACATC CATCTGAATG GGTACCCATT TCTTGATGTG 
AAGGATGAGT TCACTCACCG TACCCTAGCC ACTGATGTGA TGCGGCCCCG CAGGGGAGAA 
CCACCTTTGT CAGTACTAAC CCAGGACAGC ATGACTGTGG AGGATGTGGA GACTCTCATA 
AAAGAGACAG ACTACAATGG CTTTCCTGTG GTGGTCTCGA GAGATTCGGA GCGCCTCATA 
GGCTTTGCCC AGAGGCGGGA GCTAATCTTG GCTATAAAAA ATGCCAGACA GAGGCAAGAG 
GGCATCGTGA GCAATTCCAT CATGTACTTC ACAGAGGAGC CCCCTGAGCT GCCTGCCAAC 
AGCCCACATC CACTGAAGCT GAGGCGCATT CTGAACCTGA GCCCTTTTAC TGTCACAGAC 
CACACCCCCA TGGAGACAGT GGTGGACATT TTCCGGAAAC TGGGGCTCCG ACAATGCCTG 
GTGACACGGA GTGGGAGGCT TCTTGGGATC ATCACAAAAA AAGACGTTCT GAGACACATG 
GCGCAGATGG CAAACCAGGA CCCTGAATCC ATCATGTTTA ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

X-exome sequencing of 405 unresolved families identifies seven novel intellectual disability genes.
Molecular psychiatry21(1)133-48(2016 Jan)
Hu H,Haas SA,Chelly J,Van Esch H,Raynaud M,de Brouwer AP,Weinert S,Froyen G,Frints SG,Laumonnier F,Zemojtel T,Love MI,Richard H,Emde AK,Bienek M,Jensen C,Hambrock M,Fischer U,Langnick C,Feldkamp M,Wissink-Lindhout W,Lebrun N,Castelnau L,Rucci J,Montjean R,Dorseuil O,Billuart P,Stuhlmann T,Shaw M,Corbett MA,Gardner A,Willis-Owen S,Tan C,Friend KL,Belet S,van Roozendaal KE,Jimenez-Pocquet M,Moizard MP,Ronce N,Sun R,O'Keeffe S,Chenna R,van Bömmel A,Göke J,Hackett A,Field M,Christie L,Boyle J,Haan E,Nelson J,Turner G,Baynam G,Gillessen-Kaesbach G,Müller U,Steinberger D,Budny B,Badura-Stronka M,Latos-Bieleńska A,Ousager LB,Wieacker P,Rodríguez Criado G,Bondeson ML,Annerén G,Dufke A,Cohen M,Van Maldergem L,Vincent-Delorme C,Echenne B,Simon-Bouy B,Kleefstra T,Willemsen M,Fryns JP,Devriendt K,Ullmann R,Vingron M,Wrogemann K,Wienker TF,Tzschach A,van Bokhoven H,Gecz J,Jentsch TJ,Chen W,Ropers HH,Kalscheuer VM

Exome sequencing reveals new causal mutations in children with epileptic encephalopathies.
Epilepsia54(7)1270-81(2013 Jul)
Veeramah KR,Johnstone L,Karafet TM,Wolf D,Sprissler R,Salogiannis J,Barth-Maron A,Greenberg ME,Stuhlmann T,Weinert S,Jentsch TJ,Pazzi M,Restifo LL,Talwar D,Erickson RP,Hammer MF

The human ClC-4 protein, a member of the CLC chloride channel/transporter family, is localized to the endoplasmic reticulum by its N-terminus.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology20(13)2390-2(2006 Nov)
Okkenhaug H,Weylandt KH,Carmena D,Wells DJ,Higgins CF,Sardini A

Identification of an acid-activated Cl(-) channel from human skeletal muscles.
The American journal of physiology277(5)C948-54(1999 11)
Kawasaki M,Fukuma T,Yamauchi K,Sakamoto H,Marumo F,Sasaki S

Properties of voltage-gated chloride channels of the ClC gene family.
The Journal of physiology48219S-25S(1995 Jan)
Jentsch TJ,Günther W,Pusch M,Schwappach B

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Clcn4 cDNA ORF clone, Rattus norvegicus(Norway rat)

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