Pold1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pold1
Entrez Gene ID	59294
Full Name	DNA polymerase delta 1, catalytic subunit
General protein information	Preferred Names DNA polymerase delta 1, catalytic subunit Names DNA polymerase delta catalytic subunit DAN polymerase delta 1, catalytic subunit DNA polymerase delta, catalytic subunit DNA-directed DNA polymerase delta 1 polymerase (DNA directed), delta 1, catalytic subunit polymerase (DNA) delta 1, catalytic subunit
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q22
Summary	has DNA polymerase and 3'exonuclease activities; plays roles in DNA replication, base excision repair, and other types of DNA repair [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_021662.2	NP_067694.1	DNA polymerase delta catalytic subunit
XM_006229143.3	XP_006229205.1	DNA polymerase delta catalytic subunit isoform X1
XM_006229142.3	XP_006229204.1	DNA polymerase delta catalytic subunit isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	POLD1	NP_001243778.1
Saccharomyces cerevisiae (baker's yeast)	POL3	NP_010181.2
Xenopus tropicalis (tropical clawed frog)	pold1	NP_001039204.1
Rattus norvegicus (Norway rat)	Pold1	NP_067694.1
Danio rerio (zebrafish)	pold1	NP_001034899.1
Neurospora crassa	NCU01192	XP_961558.1
Oryza sativa (rice)	Os11g0186400	NP_001067405.1
Bos taurus (cattle)	POLD1	NP_776852.1
Mus musculus (house mouse)	Pold1	NP_035261.3
Drosophila melanogaster (fruit fly)	DNApol-delta	NP_524099.2
Caenorhabditis elegans (roundworm)	F10C2.4	NP_506017.1
Arabidopsis thaliana (thale cress)	EMB2780	NP_201201.2
Macaca mulatta (Rhesus monkey)	POLD1	XP_001116065.1
Canis lupus familiaris (dog)	POLD1	XP_851285.1
Magnaporthe oryzae (rice blast fungus)	MGG_08071	XP_003715024.1
Schizosaccharomyces pombe (fission yeast)	cdc6	NP_596124.1
Kluyveromyces lactis	KLLA0E01607g	XP_454020.1
Pan troglodytes (chimpanzee)	POLD1	XP_003316601.2
Eremothecium gossypii	AGOS_AFL189W	NP_985361.2

Gene Ontology
Bibliography

Related articles in PubMed

CBP and p300 acetylate PCNA to link its degradation with nucleotide excision repair synthesis.
Cazzalini O, Sommatis S, Tillhon M, Dutto I, Bachi A, Rapp A, Nardo T, Scovassi AI, Necchi D, Cardoso MC, Stivala LA, Prosperi E
Nucleic acids research42(13)8433-48(2014 Jul)

RTEL1 is a replisome-associated helicase that promotes telomere and genome-wide replication.
Vannier JB, Sandhu S, Petalcorin MI, Wu X, Nabi Z, Ding H, Boulton SJ
Science (New York, N.Y.)342(6155)239-42(2013 Oct)

An in-frame deletion at the polymerase active site of POLD1 causes a multisystem disorder with lipodystrophy.
Weedon MN, Ellard S, Prindle MJ, Caswell R, Lango Allen H, Oram R, Godbole K, Yajnik CS, Sbraccia P, Novelli G, Turnpenny P, McCann E, Goh KJ, Wang Y, Fulford J, McCulloch LJ, Savage DB, O'Rahilly S, Kos K, Loeb LA, Semple RK, Hattersley AT
Nature genetics45(8)947-50(2013 Aug)

A quantitative telomeric chromatin isolation protocol identifies different telomeric states.
Grolimund L, Aeby E, Hamelin R, Armand F, Chiappe D, Moniatte M, Lingner J
Nature communications42848(2013)

DNA polymerase δ and ζ switch by sharing accessory subunits of DNA polymerase δ.
Baranovskiy AG, Lada AG, Siebler HM, Zhang Y, Pavlov YI, Tahirov TH
The Journal of biological chemistry287(21)17281-7(2012 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Pold1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pold1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa04990	NM_021662.2 Latest version!	Rattus norvegicus DNA polymerase delta 1, catalytic subunit (Pold1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa22775	XM_006229142.3 Latest version!	Rattus norvegicus polymerase (DNA) delta 1, catalytic subunit (Pold1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa22775	XM_006229143.3 Latest version!	Rattus norvegicus polymerase (DNA) delta 1, catalytic subunit (Pold1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pold1 ( NM_021662.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_067694.1 Rattus norvegicus DNA polymerase delta 1, catalytic subunit (Pold1), mRNA.

Price & Availability↑

CloneID	ORa04990
Clone ID Related Accession (Same CDS sequence)	NM_021662.2
Accession Version	NM_021662.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3312bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1570550400000
Organism	Rattus norvegicus(Norway rat)
Product	DNA polymerase delta catalytic subunit
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AJ222691.1. On Aug 31, 2012 this sequence version replaced NM_021662.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ222691.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906351 [ECO:0000348] ##Evidence-Data-END##

ATGGATGGTA AACGGCGGCA AGCGCCCAGC TCTGGGGTGC CCCCAAAGCG GGCTTGCAAG 
GGCCTCTGGG ATGAAGATGA GCCGTCACAG TTTGAGGAGA ACCTGGCGCT GCTGGAGGAG 
ATAGAGGCCG AGAATCGGCT GCAGGAGGCC GAGGAGGAGC TGCAGCTGCC CCCTGAGGGC 
ATTGTGGGTG GGCAGTTTTC CACTGCAGAC ATTGACCCAC GGTGGCTGCG GCCCACCCCA 
CTTGCCCTGG ACCCCAGCAC GGAGCCCCTC ATCTTCCAGC AGCTGGAGAT TGACCACTAT 
GTGGGCACAT CACCTCCCCT GCCAGAAGGA CCCCCCGCAT CTCGTAACTC AGTGCCCATA 
CTGAGGGCCT TTGGGGTCAC CGATGAGGGC TTCTCCGTCT GCTGCCACAT CCACGGCTTT 
GCCCCCTACT TCTACACCCC TGCACCTCCG GGTTTTGGGG CTGAGCACCT GAGTGAACTA 
CAGCGGGAGC TGAATGCAGC CATCAGCCGG GACCAGCGTG GTGGAAAGGA GCTCTCGGGG 
CCGGCAGTGC TAGCTATAGA GCTGTGCTCC CGTGAGAGCA TGTTTGGGTA CCATGGCCAC 
GGCCCTTCTC CCTTTCTCCG CATCACCCTG GCACTACCCC GCCTGATGGC GCCAGCCCGC 
CGCCTCCTGG AACAGGGTAT CCGAGTGCCA GGCCTGGGCA CCCCGAGCTT TGCACCCTAT 
GAAGCCAATG TGGACTTTGA GATCCGGTTC ATGGTGGATG CTGACATTGT GGGATGCAAC 
TGGTTGGAGC TCCCAGCTGG AAAGTACGTT CGGAGGGCAG AGAAGAAGGC TACACTGTGT 
CAGCTGGAGG TGGATGTGCT GTGGTCAGAC GTGATCAGTC ACCCACCAGA AGGGCAGTGG 
CAGCGCATCG CACCCCTGCG TGTGCTTAGC TTCGACATCG AGTGCGCTGG CCGAAAAGGC 
ATCTTCCCTG AGCCTGAGCG TGACCCCGTG ATCCAGATCT GTTCTCTGGG GCTGCGCTGG 
GGTGAGCCAG AGCCCTTCTT GCGCCTAGCA CTCACGCTGC GGCCTTGCGC CCCCATCCTG 
GGTGCCAAAG TACAGAGCTA TGAACGGGAA GAAGACCTGC TCCAGGCCTG GGCCACTTTC 
ATCCTCGCCA TGGACCCTGA CGTGATCACC GGCTACAACA TTCAGAACTT TGACCTCCCC 
TACCTCATCT CTCGGGCACA AACCTTAAAG GTGGACCGAT TCCCTTTCCT GGGCCGTGTG 
ACTGGTCTCC GCTCCAACAT CCGTGACTCC TCCTTCCAAT CAAGGCAGGT GGGCCGGCGG 
GACAGTAAGG TGGTCAGCAT GGTGGGTCGC GTTCAGATGG ATATGCTGCA GGTGCTGCTT 
CGGGAGTACA AGCTCCGCTC CTACACGCTC AACGCTGTGA GCTTCCACTT CCTGGGTGAG 
CAGAAGGAGG ACGTACAGCA CAGCATCATC ACTGACCTAC AGAATGGGAA TGAACAGACG 
CGTCGCCGCC TGGCCGTGTA CTGCCTGAAG GATGCCTTTC TGCCTCTTCG CCTACTCGAG 
CGCCTTATGG TGCTGGTGAA CAATGTGGAG ATGGCGCGTG TCACTGGTGT ACCCCTTGGG 
TACCTGCTCA GCCGAGGCCA GCAGGTCAAG GTCGTGTCTC AGCTGCTGCG CCAGGCCATG 
CGCGAGGGGC TGCTGATGCC TGTGGTGAAG ACGGAGGGAG GTGAGGACTA CACGGGAGCC 
ACTGTCATTG AGCCCCTCAA AGGGTACTAT GATGTCCCCA TTGCCACCCT GGACTTCTCC 
TCTCTGTACC CATCCATCAT GATGGCCCAC AATCTGTGCT ACACCACATT GCTACGGCCT 
GGGGCTGCCC AGAAGTTGGG CCTTAAACCA GATGAGTTCA TCAAGACACC CACTGGGGAT 
GAGTTTGTGA AGGCATCTGT GCGGAAGGGC CTCCTGCCCC AAATCCTGGA GAATCTGCTG 
AGTGCCCGGA AGAGGGCCAA GGCTGAGCTG GCTCAGGAGA CGGACCCCCT GCGGCGACAG 
GTCTTGGATG GACGGCAGCT GGCACTAAAA GTGAGTCCCA ACTCTGTGTA TGGCTTCACT 
GGTGCCCAGG TGGGCAAGCT GCCGTGTTTG GAGATCTCCC AGAGTGTCAC TGGGTTCGGG 
CGGCAGATGA TTGAGAAAAC CAAGCAGCTA GTGGAGACCA AGTACACTCT GGAAAATGGC 
TACGATGCTA ATGCCAAGGT GGTCTACGGT GACACTGACT CTGTGATGTG CCGATTTGGT 
GTCTCCTCTG TGGCTGAGGC AATGTCTCTG GGGCGGGAGG CTGCAAACTG GGTATCCAGT 
CACTTCCCAT CACCCATCCG GCTGGAGTTC GAGAAGGTTT ACTTTCCCTA CCTGCTCATC 
AGCAAGAAGC GCTATGCCGG CCTACTCTTC TCCTCCCGCT CTGATGCCCA TGACAGAATG 
GACTGCAAGG GCCTGGAGGC TGTGCGTAGG GACAACTGTC CCCTGGTGGC CAACCTTGTC 
ACATCCTCTC TGCGCAGAAT CCTCGTGGAT CGGGACCCTG ATGGTGCAGT AGCCCATGCA 
AAGGATGTCA TCTCGGACCT GCTGTGCAAC CGCATAGACA TCTCCCAACT GGTCATCACC 
AAAGAGTTGA CCCGCGCAGC AGCAGACTAC GCGGGCAAGC AGGCTCATGT GGAGCTGGCT 
GAGAGGATGA GGAAGCGTGA CCCCGGCAGT GCGCCCAACT TGGGCGACCG AGTACCCTAC 
GTGATCATTG GTGCTGCCAA GGGTGTGGCC GCCTACATGA AGTCGGAGGA CCCCCTGTTT 
GTGCTGGAGC ACAGCCTGCC CATTGATACT CAGTACTACC TGGAGCAGCA GCTGGCCAAG 
CCGCTATTGC GCATCTTTGA GCCCATTCTG GGTGAGGGCC GCGCGGAGTC AGTGCTGCTG 
CGCGGTGACC ACACACGCTG CAAAACCGTG CTCACCAGCA AGGTGGGCGG CCTTCTGGCC 
TTCACCAAGC GCCGAAACTC TTGTATTGGC TGCCGCTCCG TAATCGACCA TCAAGGAGCC 
GTGTGTAAGT TCTGTCAGCC ACGGGAGTCT GAGCTCTATC AGAAGGAGGT GTCACACCTG 
AATGCCCTGG AGGAACGTTT CTCGCGCCTC TGGACACAGT GCCAGCGCTG CCAGGGCAGC 
TTGCACGAGG ATGTCATCTG TACCAGCCGC GACTGTCCCA TCTTCTACAT GCGCAAGAAG 
GTGCGCAAGG ACCTGGAGGA CCAGGAACGG CTGCTGCAGC GCTTTGGACC TCCTGGCCCT 
GAGGCCTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_067694.1
CDS	2..3313
Translation	MDGKRRQAPSSGVPPKRACKGLWDEDEPSQFEENLALLEEIEAENRLQEAEEELQLPPEGIVGGQFSTADIDPRWLRPTPLALDPSTEPLIFQQLEIDHYVGTSPPLPEGPPASRNSVPILRAFGVTDEGFSVCCHIHGFAPYFYTPAPPGFGAEHLSELQRELNAAISRDQRGGKELSGPAVLAIELCSRESMFGYHGHGPSPFLRITLALPRLMAPARRLLEQGIRVPGLGTPSFAPYEANVDFEIRFMVDADIVGCNWLELPAGKYVRRAEKKATLCQLEVDVLWSDVISHPPEGQWQRIAPLRVLSFDIECAGRKGIFPEPERDPVIQICSLGLRWGEPEPFLRLALTLRPCAPILGAKVQSYEREEDLLQAWATFILAMDPDVITGYNIQNFDLPYLISRAQTLKVDRFPFLGRVTGLRSNIRDSSFQSRQVGRRDSKVVSMVGRVQMDMLQVLLREYKLRSYTLNAVSFHFLGEQKEDVQHSIITDLQNGNEQTRRRLAVYCLKDAFLPLRLLERLMVLVNNVEMARVTGVPLGYLLSRGQQVKVVSQLLRQAMREGLLMPVVKTEGGEDYTGATVIEPLKGYYDVPIATLDFSSLYPSIMMAHNLCYTTLLRPGAAQKLGLKPDEFIKTPTGDEFVKASVRKGLLPQILENLLSARKRAKAELAQETDPLRRQVLDGRQLALKVSPNSVYGFTGAQVGKLPCLEISQSVTGFGRQMIEKTKQLVETKYTLENGYDANAKVVYGDTDSVMCRFGVSSVAEAMSLGREAANWVSSHFPSPIRLEFEKVYFPYLLISKKRYAGLLFSSRSDAHDRMDCKGLEAVRRDNCPLVANLVTSSLRRILVDRDPDGAVAHAKDVISDLLCNRIDISQLVITKELTRAAADYAGKQAHVELAERMRKRDPGSAPNLGDRVPYVIIGAAKGVAAYMKSEDPLFVLEHSLPIDTQYYLEQQLAKPLLRIFEPILGEGRAESVLLRGDHTRCKTVLTSKVGGLLAFTKRRNSCIGCRSVIDHQGAVCKFCQPRESELYQKEVSHLNALEERFSRLWTQCQRCQGSLHEDVICTSRDCPIFYMRKKVRKDLEDQERLLQRFGPPGPEAW

Target ORF information:

RefSeq Version	NM_021662.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus DNA polymerase delta 1, catalytic subunit (Pold1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021662.2

ORF Insert Sequence:

ATGGATGGTA AACGGCGGCA AGCGCCCAGC TCTGGGGTGC CCCCAAAGCG GGCTTGCAAG 
GGCCTCTGGG ATGAAGATGA GCCGTCACAG TTTGAGGAGA ACCTGGCGCT GCTGGAGGAG 
ATAGAGGCCG AGAATCGGCT GCAGGAGGCC GAGGAGGAGC TGCAGCTGCC CCCTGAGGGC 
ATTGTGGGTG GGCAGTTTTC CACTGCAGAC ATTGACCCAC GGTGGCTGCG GCCCACCCCA 
CTTGCCCTGG ACCCCAGCAC GGAGCCCCTC ATCTTCCAGC AGCTGGAGAT TGACCACTAT 
GTGGGCACAT CACCTCCCCT GCCAGAAGGA CCCCCCGCAT CTCGTAACTC AGTGCCCATA 
CTGAGGGCCT TTGGGGTCAC CGATGAGGGC TTCTCCGTCT GCTGCCACAT CCACGGCTTT 
GCCCCCTACT TCTACACCCC TGCACCTCCG GGTTTTGGGG CTGAGCACCT GAGTGAACTA 
CAGCGGGAGC TGAATGCAGC CATCAGCCGG GACCAGCGTG GTGGAAAGGA GCTCTCGGGG 
CCGGCAGTGC TAGCTATAGA GCTGTGCTCC CGTGAGAGCA TGTTTGGGTA CCATGGCCAC 
GGCCCTTCTC CCTTTCTCCG CATCACCCTG GCACTACCCC GCCTGATGGC GCCAGCCCGC 
CGCCTCCTGG AACAGGGTAT CCGAGTGCCA GGCCTGGGCA CCCCGAGCTT TGCACCCTAT 
GAAGCCAATG TGGACTTTGA GATCCGGTTC ATGGTGGATG CTGACATTGT GGGATGCAAC 
TGGTTGGAGC TCCCAGCTGG AAAGTACGTT CGGAGGGCAG AGAAGAAGGC TACACTGTGT 
CAGCTGGAGG TGGATGTGCT GTGGTCAGAC GTGATCAGTC ACCCACCAGA AGGGCAGTGG 
CAGCGCATCG CACCCCTGCG TGTGCTTAGC TTCGACATCG AGTGCGCTGG CCGAAAAGGC 
ATCTTCCCTG AGCCTGAGCG TGACCCCGTG ATCCAGATCT GTTCTCTGGG GCTGCGCTGG 
GGTGAGCCAG AGCCCTTCTT GCGCCTAGCA CTCACGCTGC GGCCTTGCGC CCCCATCCTG 
GGTGCCAAAG TACAGAGCTA TGAACGGGAA GAAGACCTGC TCCAGGCCTG GGCCACTTTC 
ATCCTCGCCA TGGACCCTGA CGTGATCACC GGCTACAACA TTCAGAACTT TGACCTCCCC 
TACCTCATCT CTCGGGCACA AACCTTAAAG GTGGACCGAT TCCCTTTCCT GGGCCGTGTG 
ACTGGTCTCC GCTCCAACAT CCGTGACTCC TCCTTCCAAT CAAGGCAGGT GGGCCGGCGG 
GACAGTAAGG TGGTCAGCAT GGTGGGTCGC GTTCAGATGG ATATGCTGCA GGTGCTGCTT 
CGGGAGTACA AGCTCCGCTC CTACACGCTC AACGCTGTGA GCTTCCACTT CCTGGGTGAG 
CAGAAGGAGG ACGTACAGCA CAGCATCATC ACTGACCTAC AGAATGGGAA TGAACAGACG 
CGTCGCCGCC TGGCCGTGTA CTGCCTGAAG GATGCCTTTC TGCCTCTTCG CCTACTCGAG 
CGCCTTATGG TGCTGGTGAA CAATGTGGAG ATGGCGCGTG TCACTGGTGT ACCCCTTGGG 
TACCTGCTCA GCCGAGGCCA GCAGGTCAAG GTCGTGTCTC AGCTGCTGCG CCAGGCCATG 
CGCGAGGGGC TGCTGATGCC TGTGGTGAAG ACGGAGGGAG GTGAGGACTA CACGGGAGCC 
ACTGTCATTG AGCCCCTCAA AGGGTACTAT GATGTCCCCA TTGCCACCCT GGACTTCTCC 
TCTCTGTACC CATCCATCAT GATGGCCCAC AATCTGTGCT ACACCACATT GCTACGGCCT 
GGGGCTGCCC AGAAGTTGGG CCTTAAACCA GATGAGTTCA TCAAGACACC CACTGGGGAT 
GAGTTTGTGA AGGCATCTGT GCGGAAGGGC CTCCTGCCCC AAATCCTGGA GAATCTGCTG 
AGTGCCCGGA AGAGGGCCAA GGCTGAGCTG GCTCAGGAGA CGGACCCCCT GCGGCGACAG 
GTCTTGGATG GACGGCAGCT GGCACTAAAA GTGAGTCCCA ACTCTGTGTA TGGCTTCACT 
GGTGCCCAGG TGGGCAAGCT GCCGTGTTTG GAGATCTCCC AGAGTGTCAC TGGGTTCGGG 
CGGCAGATGA TTGAGAAAAC CAAGCAGCTA GTGGAGACCA AGTACACTCT GGAAAATGGC 
TACGATGCTA ATGCCAAGGT GGTCTACGGT GACACTGACT CTGTGATGTG CCGATTTGGT 
GTCTCCTCTG TGGCTGAGGC AATGTCTCTG GGGCGGGAGG CTGCAAACTG GGTATCCAGT 
CACTTCCCAT CACCCATCCG GCTGGAGTTC GAGAAGGTTT ACTTTCCCTA CCTGCTCATC 
AGCAAGAAGC GCTATGCCGG CCTACTCTTC TCCTCCCGCT CTGATGCCCA TGACAGAATG 
GACTGCAAGG GCCTGGAGGC TGTGCGTAGG GACAACTGTC CCCTGGTGGC CAACCTTGTC 
ACATCCTCTC TGCGCAGAAT CCTCGTGGAT CGGGACCCTG ATGGTGCAGT AGCCCATGCA 
AAGGATGTCA TCTCGGACCT GCTGTGCAAC CGCATAGACA TCTCCCAACT GGTCATCACC 
AAAGAGTTGA CCCGCGCAGC AGCAGACTAC GCGGGCAAGC AGGCTCATGT GGAGCTGGCT 
GAGAGGATGA GGAAGCGTGA CCCCGGCAGT GCGCCCAACT TGGGCGACCG AGTACCCTAC 
GTGATCATTG GTGCTGCCAA GGGTGTGGCC GCCTACATGA AGTCGGAGGA CCCCCTGTTT 
GTGCTGGAGC ACAGCCTGCC CATTGATACT CAGTACTACC TGGAGCAGCA GCTGGCCAAG 
CCGCTATTGC GCATCTTTGA GCCCATTCTG GGTGAGGGCC GCGCGGAGTC AGTGCTGCTG 
CGCGGTGACC ACACACGCTG CAAAACCGTG CTCACCAGCA AGGTGGGCGG CCTTCTGGCC 
TTCACCAAGC GCCGAAACTC TTGTATTGGC TGCCGCTCCG TAATCGACCA TCAAGGAGCC 
GTGTGTAAGT TCTGTCAGCC ACGGGAGTCT GAGCTCTATC AGAAGGAGGT GTCACACCTG 
AATGCCCTGG AGGAACGTTT CTCGCGCCTC TGGACACAGT GCCAGCGCTG CCAGGGCAGC 
TTGCACGAGG ATGTCATCTG TACCAGCCGC GACTGTCCCA TCTTCTACAT GCGCAAGAAG 
GTGCGCAAGG ACCTGGAGGA CCAGGAACGG CTGCTGCAGC GCTTTGGACC TCCTGGCCCT 
GAGGCCTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pold1 ( XM_006229143.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006229205.1 Rattus norvegicus polymerase (DNA) delta 1, catalytic subunit (Pold1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa22775
Clone ID Related Accession (Same CDS sequence)	XM_006229143.3 , XM_006229142.3
Accession Version	XM_006229143.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3312bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	DNA polymerase delta catalytic subunit isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006229143.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGGTA AACGGCGGCA AGCGCCCAGC TCTGGGGTGC CCCCAAAGCG GGCTTGCAAG 
GGCCTCTGGG ATGAAGATGA GCCGTCACAG TTTGAGGAGA ACCTGGCGCT GCTGGAGGAG 
ATAGAGGCCG AGAATCGGCT GCAGGAGGCC GAGGAGGAGC TGCAGCTGCC CCCTGAGGGC 
ATTGTGGGTG GGCAGTTTTC CACTGCAGAC ATTGACCCAC GGTGGCTGCG GCCCACCCCA 
CTTGCCCTGG ACCCCAGCAC GGAGCCCCTC ATCTTCCAGC AGCTGGAGAT TGACCACTAT 
GTGGGCACAT CACCTCCCCT GCCAGAAGGA CCCCCCGCAT CTCGTAACTC AGTGCCCATA 
CTGAGGGCCT TTGGGGTCAC CGATGAGGGC TTCTCCGTCT GCTGCCACAT CCACGGCTTT 
GCCCCCTACT TCTACACCCC TGCACCTCCG GGTTTTGGGG CTGAGCACCT GAGTGAACTA 
CAGCGGGAGC TGAATGCAGC CATCAGCCGG GACCAGCGTG GTGGAAAGGA GCTCTCGGGG 
CCGGCAGTGC TAGCTATAGA GCTGTGCTCC CGTGAGAGCA TGTTTGGGTA CCATGGCCAC 
GGCCCTTCTC CCTTTCTCCG CATCACCCTG GCACTACCCC GCCTGATGGC GCCAGCCCGC 
CGCCTCCTGG AACAGGGTAT CCGAGTGCCA GGCCTGGGCA CCCCGAGCTT TGCACCCTAT 
GAAGCCAATG TGGACTTTGA GATCCGGTTC ATGGTGGATG CTGACATTGT GGGATGCAAC 
TGGTTGGAGC TCCCAGCTGG AAAGTACGTT CGGAGGGCAG AGAAGAAGGC TACACTGTGT 
CAGCTGGAGG TGGATGTGCT GTGGTCAGAC GTGATCAGTC ACCCACCAGA AGGGCAGTGG 
CAGCGCATCG CACCCCTGCG TGTGCTTAGC TTCGACATCG AGTGCGCTGG CCGAAAAGGC 
ATCTTCCCTG AGCCTGAGCG TGACCCCGTG ATCCAGATCT GTTCTCTGGG GCTGCGCTGG 
GGTGAGCCAG AGCCCTTCTT GCGCCTAGCA CTCACGCTGC GGCCTTGCGC CCCCATCCTG 
GGTGCCAAAG TACAGAGCTA TGAACGGGAA GAAGACCTGC TCCAGGCCTG GGCCACTTTC 
ATCCTCGCCA TGGACCCTGA CGTGATCACC GGCTACAACA TTCAGAACTT TGACCTCCCC 
TACCTCATCT CTCGGGCACA AACCTTAAAG GTGGACCGAT TCCCTTTCCT GGGCCGTGTG 
ACTGGTCTCC GCTCCAACAT CCGTGACTCC TCCTTCCAAT CAAGGCAGGT GGGCCGGCGG 
GACAGTAAGG TGGTCAGCAT GGTGGGTCGC GTTCAGATGG ATATGCTGCA GGTGCTGCTT 
CGGGAGTACA AGCTCCGCTC CTACACGCTC AACGCTGTGA GCTTCCACTT CCTGGGTGAG 
CAGAAGGAGG ACGTACAGCA CAGCATCATC ACTGACCTAC AGAATGGGAA TGAACAGACG 
CGTCGCCGCC TGGCCGTGTA CTGCCTGAAG GATGCCTTTC TGCCTCTTCG CCTACTCGAG 
CGCCTTATGG TGCTGGTGAA CAATGTGGAG ATGGCGCGTG TCACTGGTGT ACCCCTTGGG 
TACCTGCTCA GCCGAGGCCA GCAGGTCAAG GTCGTGTCTC AGCTGCTGCG CCAGGCCATG 
CGCCAGGGGC TGCTGATGCC TGTGGTGAAG ACGGAGGGAG GTGAGGACTA CACGGGAGCC 
ACTGTCATTG AGCCCCTCAA AGGGTACTAT GATGTCCCCA TTGCCACCCT GGACTTCTCC 
TCTCTGTACC CATCCATCAT GATGGCCCAC AATCTGTGCT ACACCACATT GCTACGGCCT 
GGGGCTGCCC AGAAGTTGGG CCTTAAACCA GATGAGTTCA TCAAGACACC CACTGGGGAT 
GAGTTTGTGA AGGCATCTGT GCGGAAGGGC CTCCTGCCCC AAATCCTGGA GAATCTGCTG 
AGTGCCCGGA AGAGGGCCAA GGCTGAGCTG GCTCAGGAGA CGGACCCCCT GCGGCGACAG 
GTCTTGGATG GACGGCAGCT GGCACTAAAA GTGAGTGCCA ACTCTGTGTA TGGCTTCACT 
GGTGCCCAGG TGGGCAAGCT GCCGTGTTTG GAGATCTCCC AGAGTGTCAC TGGGTTCGGG 
CGGCAGATGA TTGAGAAAAC CAAGCAGCTA GTGGAGACCA AGTACACTCT GGAAAATGGC 
TACGATGCTA ATGCCAAGGT GGTCTACGGT GACACTGACT CTGTGATGTG CCGATTTGGT 
GTCTCCTCTG TGGCTGAGGC AATGTCTCTG GGGCGGGAGG CTGCAAACTG GGTATCCAGT 
CACTTCCCAT CACCCATCCG GCTGGAGTTC GAGAAGGTTT ACTTTCCCTA CCTGCTCATC 
AGCAAGAAGC GCTATGCCGG CCTACTCTTC TCCTCCCGCT CTGATGCCCA TGACAGAATG 
GACTGCAAGG GCCTGGAGGC TGTGCGTAGG GACAACTGTC CCCTGGTGGC CAACCTTGTC 
ACATCCTCTC TGCGCAGAAT CCTCGTGGAT CGGGACCCTG ATGGTGCAGT AGCCCATGCA 
AAGGATGTCA TCTCGGACCT GCTGTGCAAC CGCATAGACA TCTCCCAACT GGTCATCACC 
AAAGAGTTGA CCCGCGCAGC AGCAGACTAC GCGGGCAAGC AGGCTCATGT GGAGCTGGCT 
GAGAGGATGA GGAAGCGTGA CCCCGGCAGT GCGCCCAACT TGGGCGACCG AGTACCCTAC 
GTGATCATTG GTGCTGCCAA GGGTGTGGCC GCCTACATGA AGTCGGAGGA CCCCCTGTTT 
GTGCTGGAGC ACAGCCTGCC CATTGATACT CAGTACTACC TGGAGCAGCA GCTGGCCAAG 
CCGCTATTGC GCATCTTTGA GCCCATTCTG GGTGAGGGCC GCGCGGAGTC AGTGCTGCTG 
CGCGGTGACC ACACACGCTG CAAAACCGTG CTCACCAGCA AGGTGGGCGG CCTTCTGGCC 
TTCACCAAGC GCCGAAACTC TTGTATTGGC TGCCGCTCCG TAATCGACCA TCAAGGAGCC 
GTGTGTAAGT TCTGTCAGCC ACGGGAGTCT GAGCTCTATC AGAAGGAGGT GTCACACCTG 
AATGCCCTGG AGGAACGTTT CTCGCGCCTC TGGACACAGT GCCAGCGCTG CCAGGGCAGC 
TTGCACGAGG ATGTCATCTG TACCAGCCGC GACTGTCCCA TCTTCTACAT GCGCAAGAAG 
GTGCGCAAGG ACCTGGAGGA CCAGGAACGG CTGCTGCAGC GCTTTGGACC TCCTGGCCCT 
GAGGCCTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006229205.1
CDS	1613..4924
Translation	MDGKRRQAPSSGVPPKRACKGLWDEDEPSQFEENLALLEEIEAENRLQEAEEELQLPPEGIVGGQFSTADIDPRWLRPTPLALDPSTEPLIFQQLEIDHYVGTSPPLPEGPPASRNSVPILRAFGVTDEGFSVCCHIHGFAPYFYTPAPPGFGAEHLSELQRELNAAISRDQRGGKELSGPAVLAIELCSRESMFGYHGHGPSPFLRITLALPRLMAPARRLLEQGIRVPGLGTPSFAPYEANVDFEIRFMVDADIVGCNWLELPAGKYVRRAEKKATLCQLEVDVLWSDVISHPPEGQWQRIAPLRVLSFDIECAGRKGIFPEPERDPVIQICSLGLRWGEPEPFLRLALTLRPCAPILGAKVQSYEREEDLLQAWATFILAMDPDVITGYNIQNFDLPYLISRAQTLKVDRFPFLGRVTGLRSNIRDSSFQSRQVGRRDSKVVSMVGRVQMDMLQVLLREYKLRSYTLNAVSFHFLGEQKEDVQHSIITDLQNGNEQTRRRLAVYCLKDAFLPLRLLERLMVLVNNVEMARVTGVPLGYLLSRGQQVKVVSQLLRQAMRQGLLMPVVKTEGGEDYTGATVIEPLKGYYDVPIATLDFSSLYPSIMMAHNLCYTTLLRPGAAQKLGLKPDEFIKTPTGDEFVKASVRKGLLPQILENLLSARKRAKAELAQETDPLRRQVLDGRQLALKVSANSVYGFTGAQVGKLPCLEISQSVTGFGRQMIEKTKQLVETKYTLENGYDANAKVVYGDTDSVMCRFGVSSVAEAMSLGREAANWVSSHFPSPIRLEFEKVYFPYLLISKKRYAGLLFSSRSDAHDRMDCKGLEAVRRDNCPLVANLVTSSLRRILVDRDPDGAVAHAKDVISDLLCNRIDISQLVITKELTRAAADYAGKQAHVELAERMRKRDPGSAPNLGDRVPYVIIGAAKGVAAYMKSEDPLFVLEHSLPIDTQYYLEQQLAKPLLRIFEPILGEGRAESVLLRGDHTRCKTVLTSKVGGLLAFTKRRNSCIGCRSVIDHQGAVCKFCQPRESELYQKEVSHLNALEERFSRLWTQCQRCQGSLHEDVICTSRDCPIFYMRKKVRKDLEDQERLLQRFGPPGPEAW

Target ORF information:

RefSeq Version	XM_006229143.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus polymerase (DNA) delta 1, catalytic subunit (Pold1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006229143.3

ORF Insert Sequence:

ATGGATGGTA AACGGCGGCA AGCGCCCAGC TCTGGGGTGC CCCCAAAGCG GGCTTGCAAG 
GGCCTCTGGG ATGAAGATGA GCCGTCACAG TTTGAGGAGA ACCTGGCGCT GCTGGAGGAG 
ATAGAGGCCG AGAATCGGCT GCAGGAGGCC GAGGAGGAGC TGCAGCTGCC CCCTGAGGGC 
ATTGTGGGTG GGCAGTTTTC CACTGCAGAC ATTGACCCAC GGTGGCTGCG GCCCACCCCA 
CTTGCCCTGG ACCCCAGCAC GGAGCCCCTC ATCTTCCAGC AGCTGGAGAT TGACCACTAT 
GTGGGCACAT CACCTCCCCT GCCAGAAGGA CCCCCCGCAT CTCGTAACTC AGTGCCCATA 
CTGAGGGCCT TTGGGGTCAC CGATGAGGGC TTCTCCGTCT GCTGCCACAT CCACGGCTTT 
GCCCCCTACT TCTACACCCC TGCACCTCCG GGTTTTGGGG CTGAGCACCT GAGTGAACTA 
CAGCGGGAGC TGAATGCAGC CATCAGCCGG GACCAGCGTG GTGGAAAGGA GCTCTCGGGG 
CCGGCAGTGC TAGCTATAGA GCTGTGCTCC CGTGAGAGCA TGTTTGGGTA CCATGGCCAC 
GGCCCTTCTC CCTTTCTCCG CATCACCCTG GCACTACCCC GCCTGATGGC GCCAGCCCGC 
CGCCTCCTGG AACAGGGTAT CCGAGTGCCA GGCCTGGGCA CCCCGAGCTT TGCACCCTAT 
GAAGCCAATG TGGACTTTGA GATCCGGTTC ATGGTGGATG CTGACATTGT GGGATGCAAC 
TGGTTGGAGC TCCCAGCTGG AAAGTACGTT CGGAGGGCAG AGAAGAAGGC TACACTGTGT 
CAGCTGGAGG TGGATGTGCT GTGGTCAGAC GTGATCAGTC ACCCACCAGA AGGGCAGTGG 
CAGCGCATCG CACCCCTGCG TGTGCTTAGC TTCGACATCG AGTGCGCTGG CCGAAAAGGC 
ATCTTCCCTG AGCCTGAGCG TGACCCCGTG ATCCAGATCT GTTCTCTGGG GCTGCGCTGG 
GGTGAGCCAG AGCCCTTCTT GCGCCTAGCA CTCACGCTGC GGCCTTGCGC CCCCATCCTG 
GGTGCCAAAG TACAGAGCTA TGAACGGGAA GAAGACCTGC TCCAGGCCTG GGCCACTTTC 
ATCCTCGCCA TGGACCCTGA CGTGATCACC GGCTACAACA TTCAGAACTT TGACCTCCCC 
TACCTCATCT CTCGGGCACA AACCTTAAAG GTGGACCGAT TCCCTTTCCT GGGCCGTGTG 
ACTGGTCTCC GCTCCAACAT CCGTGACTCC TCCTTCCAAT CAAGGCAGGT GGGCCGGCGG 
GACAGTAAGG TGGTCAGCAT GGTGGGTCGC GTTCAGATGG ATATGCTGCA GGTGCTGCTT 
CGGGAGTACA AGCTCCGCTC CTACACGCTC AACGCTGTGA GCTTCCACTT CCTGGGTGAG 
CAGAAGGAGG ACGTACAGCA CAGCATCATC ACTGACCTAC AGAATGGGAA TGAACAGACG 
CGTCGCCGCC TGGCCGTGTA CTGCCTGAAG GATGCCTTTC TGCCTCTTCG CCTACTCGAG 
CGCCTTATGG TGCTGGTGAA CAATGTGGAG ATGGCGCGTG TCACTGGTGT ACCCCTTGGG 
TACCTGCTCA GCCGAGGCCA GCAGGTCAAG GTCGTGTCTC AGCTGCTGCG CCAGGCCATG 
CGCCAGGGGC TGCTGATGCC TGTGGTGAAG ACGGAGGGAG GTGAGGACTA CACGGGAGCC 
ACTGTCATTG AGCCCCTCAA AGGGTACTAT GATGTCCCCA TTGCCACCCT GGACTTCTCC 
TCTCTGTACC CATCCATCAT GATGGCCCAC AATCTGTGCT ACACCACATT GCTACGGCCT 
GGGGCTGCCC AGAAGTTGGG CCTTAAACCA GATGAGTTCA TCAAGACACC CACTGGGGAT 
GAGTTTGTGA AGGCATCTGT GCGGAAGGGC CTCCTGCCCC AAATCCTGGA GAATCTGCTG 
AGTGCCCGGA AGAGGGCCAA GGCTGAGCTG GCTCAGGAGA CGGACCCCCT GCGGCGACAG 
GTCTTGGATG GACGGCAGCT GGCACTAAAA GTGAGTGCCA ACTCTGTGTA TGGCTTCACT 
GGTGCCCAGG TGGGCAAGCT GCCGTGTTTG GAGATCTCCC AGAGTGTCAC TGGGTTCGGG 
CGGCAGATGA TTGAGAAAAC CAAGCAGCTA GTGGAGACCA AGTACACTCT GGAAAATGGC 
TACGATGCTA ATGCCAAGGT GGTCTACGGT GACACTGACT CTGTGATGTG CCGATTTGGT 
GTCTCCTCTG TGGCTGAGGC AATGTCTCTG GGGCGGGAGG CTGCAAACTG GGTATCCAGT 
CACTTCCCAT CACCCATCCG GCTGGAGTTC GAGAAGGTTT ACTTTCCCTA CCTGCTCATC 
AGCAAGAAGC GCTATGCCGG CCTACTCTTC TCCTCCCGCT CTGATGCCCA TGACAGAATG 
GACTGCAAGG GCCTGGAGGC TGTGCGTAGG GACAACTGTC CCCTGGTGGC CAACCTTGTC 
ACATCCTCTC TGCGCAGAAT CCTCGTGGAT CGGGACCCTG ATGGTGCAGT AGCCCATGCA 
AAGGATGTCA TCTCGGACCT GCTGTGCAAC CGCATAGACA TCTCCCAACT GGTCATCACC 
AAAGAGTTGA CCCGCGCAGC AGCAGACTAC GCGGGCAAGC AGGCTCATGT GGAGCTGGCT 
GAGAGGATGA GGAAGCGTGA CCCCGGCAGT GCGCCCAACT TGGGCGACCG AGTACCCTAC 
GTGATCATTG GTGCTGCCAA GGGTGTGGCC GCCTACATGA AGTCGGAGGA CCCCCTGTTT 
GTGCTGGAGC ACAGCCTGCC CATTGATACT CAGTACTACC TGGAGCAGCA GCTGGCCAAG 
CCGCTATTGC GCATCTTTGA GCCCATTCTG GGTGAGGGCC GCGCGGAGTC AGTGCTGCTG 
CGCGGTGACC ACACACGCTG CAAAACCGTG CTCACCAGCA AGGTGGGCGG CCTTCTGGCC 
TTCACCAAGC GCCGAAACTC TTGTATTGGC TGCCGCTCCG TAATCGACCA TCAAGGAGCC 
GTGTGTAAGT TCTGTCAGCC ACGGGAGTCT GAGCTCTATC AGAAGGAGGT GTCACACCTG 
AATGCCCTGG AGGAACGTTT CTCGCGCCTC TGGACACAGT GCCAGCGCTG CCAGGGCAGC 
TTGCACGAGG ATGTCATCTG TACCAGCCGC GACTGTCCCA TCTTCTACAT GCGCAAGAAG 
GTGCGCAAGG ACCTGGAGGA CCAGGAACGG CTGCTGCAGC GCTTTGGACC TCCTGGCCCT 
GAGGCCTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pold1 ( XM_006229142.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006229204.1 Rattus norvegicus polymerase (DNA) delta 1, catalytic subunit (Pold1), transcript variant X1, mRNA.

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CloneID	ORa22775
Clone ID Related Accession (Same CDS sequence)	XM_006229143.3 , XM_006229142.3
Accession Version	XM_006229142.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3312bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	DNA polymerase delta catalytic subunit isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006229142.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGGTA AACGGCGGCA AGCGCCCAGC TCTGGGGTGC CCCCAAAGCG GGCTTGCAAG 
GGCCTCTGGG ATGAAGATGA GCCGTCACAG TTTGAGGAGA ACCTGGCGCT GCTGGAGGAG 
ATAGAGGCCG AGAATCGGCT GCAGGAGGCC GAGGAGGAGC TGCAGCTGCC CCCTGAGGGC 
ATTGTGGGTG GGCAGTTTTC CACTGCAGAC ATTGACCCAC GGTGGCTGCG GCCCACCCCA 
CTTGCCCTGG ACCCCAGCAC GGAGCCCCTC ATCTTCCAGC AGCTGGAGAT TGACCACTAT 
GTGGGCACAT CACCTCCCCT GCCAGAAGGA CCCCCCGCAT CTCGTAACTC AGTGCCCATA 
CTGAGGGCCT TTGGGGTCAC CGATGAGGGC TTCTCCGTCT GCTGCCACAT CCACGGCTTT 
GCCCCCTACT TCTACACCCC TGCACCTCCG GGTTTTGGGG CTGAGCACCT GAGTGAACTA 
CAGCGGGAGC TGAATGCAGC CATCAGCCGG GACCAGCGTG GTGGAAAGGA GCTCTCGGGG 
CCGGCAGTGC TAGCTATAGA GCTGTGCTCC CGTGAGAGCA TGTTTGGGTA CCATGGCCAC 
GGCCCTTCTC CCTTTCTCCG CATCACCCTG GCACTACCCC GCCTGATGGC GCCAGCCCGC 
CGCCTCCTGG AACAGGGTAT CCGAGTGCCA GGCCTGGGCA CCCCGAGCTT TGCACCCTAT 
GAAGCCAATG TGGACTTTGA GATCCGGTTC ATGGTGGATG CTGACATTGT GGGATGCAAC 
TGGTTGGAGC TCCCAGCTGG AAAGTACGTT CGGAGGGCAG AGAAGAAGGC TACACTGTGT 
CAGCTGGAGG TGGATGTGCT GTGGTCAGAC GTGATCAGTC ACCCACCAGA AGGGCAGTGG 
CAGCGCATCG CACCCCTGCG TGTGCTTAGC TTCGACATCG AGTGCGCTGG CCGAAAAGGC 
ATCTTCCCTG AGCCTGAGCG TGACCCCGTG ATCCAGATCT GTTCTCTGGG GCTGCGCTGG 
GGTGAGCCAG AGCCCTTCTT GCGCCTAGCA CTCACGCTGC GGCCTTGCGC CCCCATCCTG 
GGTGCCAAAG TACAGAGCTA TGAACGGGAA GAAGACCTGC TCCAGGCCTG GGCCACTTTC 
ATCCTCGCCA TGGACCCTGA CGTGATCACC GGCTACAACA TTCAGAACTT TGACCTCCCC 
TACCTCATCT CTCGGGCACA AACCTTAAAG GTGGACCGAT TCCCTTTCCT GGGCCGTGTG 
ACTGGTCTCC GCTCCAACAT CCGTGACTCC TCCTTCCAAT CAAGGCAGGT GGGCCGGCGG 
GACAGTAAGG TGGTCAGCAT GGTGGGTCGC GTTCAGATGG ATATGCTGCA GGTGCTGCTT 
CGGGAGTACA AGCTCCGCTC CTACACGCTC AACGCTGTGA GCTTCCACTT CCTGGGTGAG 
CAGAAGGAGG ACGTACAGCA CAGCATCATC ACTGACCTAC AGAATGGGAA TGAACAGACG 
CGTCGCCGCC TGGCCGTGTA CTGCCTGAAG GATGCCTTTC TGCCTCTTCG CCTACTCGAG 
CGCCTTATGG TGCTGGTGAA CAATGTGGAG ATGGCGCGTG TCACTGGTGT ACCCCTTGGG 
TACCTGCTCA GCCGAGGCCA GCAGGTCAAG GTCGTGTCTC AGCTGCTGCG CCAGGCCATG 
CGCCAGGGGC TGCTGATGCC TGTGGTGAAG ACGGAGGGAG GTGAGGACTA CACGGGAGCC 
ACTGTCATTG AGCCCCTCAA AGGGTACTAT GATGTCCCCA TTGCCACCCT GGACTTCTCC 
TCTCTGTACC CATCCATCAT GATGGCCCAC AATCTGTGCT ACACCACATT GCTACGGCCT 
GGGGCTGCCC AGAAGTTGGG CCTTAAACCA GATGAGTTCA TCAAGACACC CACTGGGGAT 
GAGTTTGTGA AGGCATCTGT GCGGAAGGGC CTCCTGCCCC AAATCCTGGA GAATCTGCTG 
AGTGCCCGGA AGAGGGCCAA GGCTGAGCTG GCTCAGGAGA CGGACCCCCT GCGGCGACAG 
GTCTTGGATG GACGGCAGCT GGCACTAAAA GTGAGTGCCA ACTCTGTGTA TGGCTTCACT 
GGTGCCCAGG TGGGCAAGCT GCCGTGTTTG GAGATCTCCC AGAGTGTCAC TGGGTTCGGG 
CGGCAGATGA TTGAGAAAAC CAAGCAGCTA GTGGAGACCA AGTACACTCT GGAAAATGGC 
TACGATGCTA ATGCCAAGGT GGTCTACGGT GACACTGACT CTGTGATGTG CCGATTTGGT 
GTCTCCTCTG TGGCTGAGGC AATGTCTCTG GGGCGGGAGG CTGCAAACTG GGTATCCAGT 
CACTTCCCAT CACCCATCCG GCTGGAGTTC GAGAAGGTTT ACTTTCCCTA CCTGCTCATC 
AGCAAGAAGC GCTATGCCGG CCTACTCTTC TCCTCCCGCT CTGATGCCCA TGACAGAATG 
GACTGCAAGG GCCTGGAGGC TGTGCGTAGG GACAACTGTC CCCTGGTGGC CAACCTTGTC 
ACATCCTCTC TGCGCAGAAT CCTCGTGGAT CGGGACCCTG ATGGTGCAGT AGCCCATGCA 
AAGGATGTCA TCTCGGACCT GCTGTGCAAC CGCATAGACA TCTCCCAACT GGTCATCACC 
AAAGAGTTGA CCCGCGCAGC AGCAGACTAC GCGGGCAAGC AGGCTCATGT GGAGCTGGCT 
GAGAGGATGA GGAAGCGTGA CCCCGGCAGT GCGCCCAACT TGGGCGACCG AGTACCCTAC 
GTGATCATTG GTGCTGCCAA GGGTGTGGCC GCCTACATGA AGTCGGAGGA CCCCCTGTTT 
GTGCTGGAGC ACAGCCTGCC CATTGATACT CAGTACTACC TGGAGCAGCA GCTGGCCAAG 
CCGCTATTGC GCATCTTTGA GCCCATTCTG GGTGAGGGCC GCGCGGAGTC AGTGCTGCTG 
CGCGGTGACC ACACACGCTG CAAAACCGTG CTCACCAGCA AGGTGGGCGG CCTTCTGGCC 
TTCACCAAGC GCCGAAACTC TTGTATTGGC TGCCGCTCCG TAATCGACCA TCAAGGAGCC 
GTGTGTAAGT TCTGTCAGCC ACGGGAGTCT GAGCTCTATC AGAAGGAGGT GTCACACCTG 
AATGCCCTGG AGGAACGTTT CTCGCGCCTC TGGACACAGT GCCAGCGCTG CCAGGGCAGC 
TTGCACGAGG ATGTCATCTG TACCAGCCGC GACTGTCCCA TCTTCTACAT GCGCAAGAAG 
GTGCGCAAGG ACCTGGAGGA CCAGGAACGG CTGCTGCAGC GCTTTGGACC TCCTGGCCCT 
GAGGCCTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006229204.1
CDS	103..3414
Translation	MDGKRRQAPSSGVPPKRACKGLWDEDEPSQFEENLALLEEIEAENRLQEAEEELQLPPEGIVGGQFSTADIDPRWLRPTPLALDPSTEPLIFQQLEIDHYVGTSPPLPEGPPASRNSVPILRAFGVTDEGFSVCCHIHGFAPYFYTPAPPGFGAEHLSELQRELNAAISRDQRGGKELSGPAVLAIELCSRESMFGYHGHGPSPFLRITLALPRLMAPARRLLEQGIRVPGLGTPSFAPYEANVDFEIRFMVDADIVGCNWLELPAGKYVRRAEKKATLCQLEVDVLWSDVISHPPEGQWQRIAPLRVLSFDIECAGRKGIFPEPERDPVIQICSLGLRWGEPEPFLRLALTLRPCAPILGAKVQSYEREEDLLQAWATFILAMDPDVITGYNIQNFDLPYLISRAQTLKVDRFPFLGRVTGLRSNIRDSSFQSRQVGRRDSKVVSMVGRVQMDMLQVLLREYKLRSYTLNAVSFHFLGEQKEDVQHSIITDLQNGNEQTRRRLAVYCLKDAFLPLRLLERLMVLVNNVEMARVTGVPLGYLLSRGQQVKVVSQLLRQAMRQGLLMPVVKTEGGEDYTGATVIEPLKGYYDVPIATLDFSSLYPSIMMAHNLCYTTLLRPGAAQKLGLKPDEFIKTPTGDEFVKASVRKGLLPQILENLLSARKRAKAELAQETDPLRRQVLDGRQLALKVSANSVYGFTGAQVGKLPCLEISQSVTGFGRQMIEKTKQLVETKYTLENGYDANAKVVYGDTDSVMCRFGVSSVAEAMSLGREAANWVSSHFPSPIRLEFEKVYFPYLLISKKRYAGLLFSSRSDAHDRMDCKGLEAVRRDNCPLVANLVTSSLRRILVDRDPDGAVAHAKDVISDLLCNRIDISQLVITKELTRAAADYAGKQAHVELAERMRKRDPGSAPNLGDRVPYVIIGAAKGVAAYMKSEDPLFVLEHSLPIDTQYYLEQQLAKPLLRIFEPILGEGRAESVLLRGDHTRCKTVLTSKVGGLLAFTKRRNSCIGCRSVIDHQGAVCKFCQPRESELYQKEVSHLNALEERFSRLWTQCQRCQGSLHEDVICTSRDCPIFYMRKKVRKDLEDQERLLQRFGPPGPEAW

Target ORF information:

RefSeq Version	XM_006229142.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus polymerase (DNA) delta 1, catalytic subunit (Pold1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006229142.3

ORF Insert Sequence:

ATGGATGGTA AACGGCGGCA AGCGCCCAGC TCTGGGGTGC CCCCAAAGCG GGCTTGCAAG 
GGCCTCTGGG ATGAAGATGA GCCGTCACAG TTTGAGGAGA ACCTGGCGCT GCTGGAGGAG 
ATAGAGGCCG AGAATCGGCT GCAGGAGGCC GAGGAGGAGC TGCAGCTGCC CCCTGAGGGC 
ATTGTGGGTG GGCAGTTTTC CACTGCAGAC ATTGACCCAC GGTGGCTGCG GCCCACCCCA 
CTTGCCCTGG ACCCCAGCAC GGAGCCCCTC ATCTTCCAGC AGCTGGAGAT TGACCACTAT 
GTGGGCACAT CACCTCCCCT GCCAGAAGGA CCCCCCGCAT CTCGTAACTC AGTGCCCATA 
CTGAGGGCCT TTGGGGTCAC CGATGAGGGC TTCTCCGTCT GCTGCCACAT CCACGGCTTT 
GCCCCCTACT TCTACACCCC TGCACCTCCG GGTTTTGGGG CTGAGCACCT GAGTGAACTA 
CAGCGGGAGC TGAATGCAGC CATCAGCCGG GACCAGCGTG GTGGAAAGGA GCTCTCGGGG 
CCGGCAGTGC TAGCTATAGA GCTGTGCTCC CGTGAGAGCA TGTTTGGGTA CCATGGCCAC 
GGCCCTTCTC CCTTTCTCCG CATCACCCTG GCACTACCCC GCCTGATGGC GCCAGCCCGC 
CGCCTCCTGG AACAGGGTAT CCGAGTGCCA GGCCTGGGCA CCCCGAGCTT TGCACCCTAT 
GAAGCCAATG TGGACTTTGA GATCCGGTTC ATGGTGGATG CTGACATTGT GGGATGCAAC 
TGGTTGGAGC TCCCAGCTGG AAAGTACGTT CGGAGGGCAG AGAAGAAGGC TACACTGTGT 
CAGCTGGAGG TGGATGTGCT GTGGTCAGAC GTGATCAGTC ACCCACCAGA AGGGCAGTGG 
CAGCGCATCG CACCCCTGCG TGTGCTTAGC TTCGACATCG AGTGCGCTGG CCGAAAAGGC 
ATCTTCCCTG AGCCTGAGCG TGACCCCGTG ATCCAGATCT GTTCTCTGGG GCTGCGCTGG 
GGTGAGCCAG AGCCCTTCTT GCGCCTAGCA CTCACGCTGC GGCCTTGCGC CCCCATCCTG 
GGTGCCAAAG TACAGAGCTA TGAACGGGAA GAAGACCTGC TCCAGGCCTG GGCCACTTTC 
ATCCTCGCCA TGGACCCTGA CGTGATCACC GGCTACAACA TTCAGAACTT TGACCTCCCC 
TACCTCATCT CTCGGGCACA AACCTTAAAG GTGGACCGAT TCCCTTTCCT GGGCCGTGTG 
ACTGGTCTCC GCTCCAACAT CCGTGACTCC TCCTTCCAAT CAAGGCAGGT GGGCCGGCGG 
GACAGTAAGG TGGTCAGCAT GGTGGGTCGC GTTCAGATGG ATATGCTGCA GGTGCTGCTT 
CGGGAGTACA AGCTCCGCTC CTACACGCTC AACGCTGTGA GCTTCCACTT CCTGGGTGAG 
CAGAAGGAGG ACGTACAGCA CAGCATCATC ACTGACCTAC AGAATGGGAA TGAACAGACG 
CGTCGCCGCC TGGCCGTGTA CTGCCTGAAG GATGCCTTTC TGCCTCTTCG CCTACTCGAG 
CGCCTTATGG TGCTGGTGAA CAATGTGGAG ATGGCGCGTG TCACTGGTGT ACCCCTTGGG 
TACCTGCTCA GCCGAGGCCA GCAGGTCAAG GTCGTGTCTC AGCTGCTGCG CCAGGCCATG 
CGCCAGGGGC TGCTGATGCC TGTGGTGAAG ACGGAGGGAG GTGAGGACTA CACGGGAGCC 
ACTGTCATTG AGCCCCTCAA AGGGTACTAT GATGTCCCCA TTGCCACCCT GGACTTCTCC 
TCTCTGTACC CATCCATCAT GATGGCCCAC AATCTGTGCT ACACCACATT GCTACGGCCT 
GGGGCTGCCC AGAAGTTGGG CCTTAAACCA GATGAGTTCA TCAAGACACC CACTGGGGAT 
GAGTTTGTGA AGGCATCTGT GCGGAAGGGC CTCCTGCCCC AAATCCTGGA GAATCTGCTG 
AGTGCCCGGA AGAGGGCCAA GGCTGAGCTG GCTCAGGAGA CGGACCCCCT GCGGCGACAG 
GTCTTGGATG GACGGCAGCT GGCACTAAAA GTGAGTGCCA ACTCTGTGTA TGGCTTCACT 
GGTGCCCAGG TGGGCAAGCT GCCGTGTTTG GAGATCTCCC AGAGTGTCAC TGGGTTCGGG 
CGGCAGATGA TTGAGAAAAC CAAGCAGCTA GTGGAGACCA AGTACACTCT GGAAAATGGC 
TACGATGCTA ATGCCAAGGT GGTCTACGGT GACACTGACT CTGTGATGTG CCGATTTGGT 
GTCTCCTCTG TGGCTGAGGC AATGTCTCTG GGGCGGGAGG CTGCAAACTG GGTATCCAGT 
CACTTCCCAT CACCCATCCG GCTGGAGTTC GAGAAGGTTT ACTTTCCCTA CCTGCTCATC 
AGCAAGAAGC GCTATGCCGG CCTACTCTTC TCCTCCCGCT CTGATGCCCA TGACAGAATG 
GACTGCAAGG GCCTGGAGGC TGTGCGTAGG GACAACTGTC CCCTGGTGGC CAACCTTGTC 
ACATCCTCTC TGCGCAGAAT CCTCGTGGAT CGGGACCCTG ATGGTGCAGT AGCCCATGCA 
AAGGATGTCA TCTCGGACCT GCTGTGCAAC CGCATAGACA TCTCCCAACT GGTCATCACC 
AAAGAGTTGA CCCGCGCAGC AGCAGACTAC GCGGGCAAGC AGGCTCATGT GGAGCTGGCT 
GAGAGGATGA GGAAGCGTGA CCCCGGCAGT GCGCCCAACT TGGGCGACCG AGTACCCTAC 
GTGATCATTG GTGCTGCCAA GGGTGTGGCC GCCTACATGA AGTCGGAGGA CCCCCTGTTT 
GTGCTGGAGC ACAGCCTGCC CATTGATACT CAGTACTACC TGGAGCAGCA GCTGGCCAAG 
CCGCTATTGC GCATCTTTGA GCCCATTCTG GGTGAGGGCC GCGCGGAGTC AGTGCTGCTG 
CGCGGTGACC ACACACGCTG CAAAACCGTG CTCACCAGCA AGGTGGGCGG CCTTCTGGCC 
TTCACCAAGC GCCGAAACTC TTGTATTGGC TGCCGCTCCG TAATCGACCA TCAAGGAGCC 
GTGTGTAAGT TCTGTCAGCC ACGGGAGTCT GAGCTCTATC AGAAGGAGGT GTCACACCTG 
AATGCCCTGG AGGAACGTTT CTCGCGCCTC TGGACACAGT GCCAGCGCTG CCAGGGCAGC 
TTGCACGAGG ATGTCATCTG TACCAGCCGC GACTGTCCCA TCTTCTACAT GCGCAAGAAG 
GTGCGCAAGG ACCTGGAGGA CCAGGAACGG CTGCTGCAGC GCTTTGGACC TCCTGGCCCT 
GAGGCCTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

CBP and p300 acetylate PCNA to link its degradation with nucleotide excision repair synthesis.
Nucleic acids research42(13)8433-48(2014 Jul)
Cazzalini O,Sommatis S,Tillhon M,Dutto I,Bachi A,Rapp A,Nardo T,Scovassi AI,Necchi D,Cardoso MC,Stivala LA,Prosperi E

RTEL1 is a replisome-associated helicase that promotes telomere and genome-wide replication.
Science (New York, N.Y.)342(6155)239-42(2013 Oct)
Vannier JB,Sandhu S,Petalcorin MI,Wu X,Nabi Z,Ding H,Boulton SJ

An in-frame deletion at the polymerase active site of POLD1 causes a multisystem disorder with lipodystrophy.
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Pold1 cDNA ORF clone, Rattus norvegicus(Norway rat)

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