Psmb4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Psmb4
Entrez Gene ID	58854
Full Name	proteasome subunit beta 4
General protein information	Preferred Names proteasome subunit beta 4 Names proteasome subunit beta type-4 RN3 macropain beta chain multicatalytic endopeptidase complex beta chain proteasome (prosome, macropain) subunit, beta type 4 proteasome beta 4 subunit proteasome beta chain proteasome chain 3
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q34
Summary	beta-type subgroup component of the proteasome multicatalytic proteinase complex [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_031629.2	NP_113817.2	proteasome subunit beta type-4

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PSMB4	XP_003949593.1
Rattus norvegicus (Norway rat)	Psmb4	NP_113817.1
Drosophila melanogaster (fruit fly)	Prosbeta7	NP_649529.1
Arabidopsis thaliana (thale cress)	PBG1	NP_176040.1
Xenopus tropicalis (tropical clawed frog)	psmb4	NP_001011182.1
Neurospora crassa	NCU07365	XP_957993.1
Macaca mulatta (Rhesus monkey)	PSMB4	XP_002801820.1
Schizosaccharomyces pombe (fission yeast)	SPBC577.10	NP_595308.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011444	XP_317860.3
Magnaporthe oryzae (rice blast fungus)	MGG_00331	XP_003718754.1
Canis lupus familiaris (dog)	PSMB4	XP_533057.3
Mus musculus (house mouse)	Psmb4	NP_032971.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012608	XP_306986.3
Kluyveromyces lactis	KLLA0D11506g	XP_453574.1
Eremothecium gossypii	AGOS_AFR258W	NP_985805.1
Oryza sativa (rice)	Os09g0515200	NP_001063665.1
Homo sapiens (human)	PSMB4	NP_002787.2
Bos taurus (cattle)	PSMB4	NP_001029438.1
Gallus gallus (chicken)	PSMB4	XP_427542.4
Danio rerio (zebrafish)	psmb4	NP_001017801.1
Saccharomyces cerevisiae (baker's yeast)	PRE4	NP_116708.1

Gene Ontology
Bibliography

Related articles in PubMed

Upregulation of PSMB4 is Associated with the Necroptosis after Spinal Cord Injury.
Wu C, Chen J, Liu Y, Zhang J, Ding W, Wang S, Bao G, Xu G, Sun Y, Wang L, Chen L, Gu H, Cui B, Cui Z
Neurochemical research41(11)3103-3112(2016 Nov)

Additive loss-of-function proteasome subunit mutations in CANDLE/PRAAS patients promote type I IFN production.
Brehm A, Liu Y, Sheikh A, Marrero B, Omoyinmi E, Zhou Q, Montealegre G, Biancotto A, Reinhardt A, Almeida de Jesus A, Pelletier M, Tsai WL, Remmers EF, Kardava L, Hill S, Kim H, Lachmann HJ, Megarbane A, Chae JJ, Brady J, Castillo RD, Brown D, Casano AV, Gao L, Chapelle D, Huang Y, Stone D, Chen Y, Sotzny F, Lee CC, Kastner DL, Torrelo A, Zlotogorski A, Moir S, Gadina M, McCoy P, Wesley R, Rother KI, Rother K, Hildebrand PW, Brogan P, Krüger E, Aksentijevich I, Goldbach-Mansky R
The Journal of clinical investigation125(11)4196-211(2015 Nov)

Proteomic characterization of the human sperm nucleus.
de Mateo S, Castillo J, Estanyol JM, Ballescà JL, Oliva R
Proteomics11(13)2714-26(2011 Jul)

MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis.
Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W
Immunology and cell biology88(8)851-6(2010 Nov-Dec)

PAC1 gene knockout reveals an essential role of chaperone-mediated 20S proteasome biogenesis and latent 20S proteasomes in cellular homeostasis.
Sasaki K, Hamazaki J, Koike M, Hirano Y, Komatsu M, Uchiyama Y, Tanaka K, Murata S
Molecular and cellular biology30(15)3864-74(2010 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Psmb4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Psmb4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa43461	NM_031629.2 Latest version!	Rattus norvegicus proteasome 20S subunit beta 4 (Psmb4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Psmb4 ( NM_031629.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_113817.2 Rattus norvegicus proteasome 20S subunit beta 4 (Psmb4), mRNA.

Price & Availability↑

CloneID	ORa43461
Clone ID Related Accession (Same CDS sequence)	NM_031629.2
Accession Version	NM_031629.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 795bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577462400000
Organism	Rattus norvegicus(Norway rat)
Product	proteasome subunit beta type-4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from FQ232850.1. On May 5, 2015 this sequence version replaced NM_031629.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: FQ227250.1, FQ232850.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906347, SAMN01906352 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAGCGT TTTGGGAGTC ACGGACTGGC CACTGGGCCG GGGGTCCGGC TCCGGGGCAG 
TTTTACCGCG TCCCGTCCAC CCCCAGCTGC CTCATGGATC CGATGTCTGC GCCCTGCGAG 
GGTCCCATCA CTCGGACCCA GAACCCCATG GTGACTGGGA CATCCGTACT AGGGGTGAAG 
TTCGACTGCG GAGTGGTGAT TGCTGCAGAC ATGCTGGGCT CCTACGGCTC CCTGGCTCGT 
TTCCGCAATA TCTCTCGTAT TATGCGAGTC AACGACAGCA CTATGCTAGG TGCTTCAGGA 
GACTATGCTG ATTTCCAGTA TTTGAAGCAA GTTCTCGGCC AGATGGTGAT TGATGAGGAG 
CTGTTGGGAG ATGGACATAG CTATAGTCCT AGAGCTATCC ATTCATGGTT GACAAGGGCC 
ATGTACAGTC GCCGCTCCAA GATGAACCCT CTGTGGAACA CCATGGTCAT TGGAGGCTAT 
GCTGGTGGAG AAAGCTTTCT TGGTTATGTG GACATGCTTG GTGTAGCTTA TGAAGCCCCT 
TCACTAGCCA CTGGTTATGG TGCATACTTG GCTCAGCCTC TGCTTCGAGA AGTTCTAGAG 
AAGCAACCGG TGCTGAGTCA GACAGAGGCC CGAGAGCTTG TGGAGCGCTG CATGCGAGTG 
CTGTACTACC GAGACGCTCG TTCGTATAAC CGGTTTCAAG TTGCTACTGT AACTGAAAAA 
GGTGTGGAGA TAGAAGGACC ACTGTCAGCA CAGACCAACT GGGACATTGC TCACATGATC 
AGTGGCTTTG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_113817.2
CDS	26..820
Translation	MEAFWESRTGHWAGGPAPGQFYRVPSTPSCLMDPMSAPCEGPITRTQNPMVTGTSVLGVKFDCGVVIAADMLGSYGSLARFRNISRIMRVNDSTMLGASGDYADFQYLKQVLGQMVIDEELLGDGHSYSPRAIHSWLTRAMYSRRSKMNPLWNTMVIGGYAGGESFLGYVDMLGVAYEAPSLATGYGAYLAQPLLREVLEKQPVLSQTEARELVERCMRVLYYRDARSYNRFQVATVTEKGVEIEGPLSAQTNWDIAHMISGFE

Target ORF information:

RefSeq Version	NM_031629.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus proteasome 20S subunit beta 4 (Psmb4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031629.2

ORF Insert Sequence:

ATGGAAGCGT TTTGGGAGTC ACGGACTGGC CACTGGGCCG GGGGTCCGGC TCCGGGGCAG 
TTTTACCGCG TCCCGTCCAC CCCCAGCTGC CTCATGGATC CGATGTCTGC GCCCTGCGAG 
GGTCCCATCA CTCGGACCCA GAACCCCATG GTGACTGGGA CATCCGTACT AGGGGTGAAG 
TTCGACTGCG GAGTGGTGAT TGCTGCAGAC ATGCTGGGCT CCTACGGCTC CCTGGCTCGT 
TTCCGCAATA TCTCTCGTAT TATGCGAGTC AACGACAGCA CTATGCTAGG TGCTTCAGGA 
GACTATGCTG ATTTCCAGTA TTTGAAGCAA GTTCTCGGCC AGATGGTGAT TGATGAGGAG 
CTGTTGGGAG ATGGACATAG CTATAGTCCT AGAGCTATCC ATTCATGGTT GACAAGGGCC 
ATGTACAGTC GCCGCTCCAA GATGAACCCT CTGTGGAACA CCATGGTCAT TGGAGGCTAT 
GCTGGTGGAG AAAGCTTTCT TGGTTATGTG GACATGCTTG GTGTAGCTTA TGAAGCCCCT 
TCACTAGCCA CTGGTTATGG TGCATACTTG GCTCAGCCTC TGCTTCGAGA AGTTCTAGAG 
AAGCAACCGG TGCTGAGTCA GACAGAGGCC CGAGAGCTTG TGGAGCGCTG CATGCGAGTG 
CTGTACTACC GAGACGCTCG TTCGTATAAC CGGTTTCAAG TTGCTACTGT AACTGAAAAA 
GGTGTGGAGA TAGAAGGACC ACTGTCAGCA CAGACCAACT GGGACATTGC TCACATGATC 
AGTGGCTTTG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Upregulation of PSMB4 is Associated with the Necroptosis after Spinal Cord Injury.
Neurochemical research41(11)3103-3112(2016 Nov)
Wu C,Chen J,Liu Y,Zhang J,Ding W,Wang S,Bao G,Xu G,Sun Y,Wang L,Chen L,Gu H,Cui B,Cui Z

Additive loss-of-function proteasome subunit mutations in CANDLE/PRAAS patients promote type I IFN production.
The Journal of clinical investigation125(11)4196-211(2015 Nov)
Brehm A,Liu Y,Sheikh A,Marrero B,Omoyinmi E,Zhou Q,Montealegre G,Biancotto A,Reinhardt A,Almeida de Jesus A,Pelletier M,Tsai WL,Remmers EF,Kardava L,Hill S,Kim H,Lachmann HJ,Megarbane A,Chae JJ,Brady J,Castillo RD,Brown D,Casano AV,Gao L,Chapelle D,Huang Y,Stone D,Chen Y,Sotzny F,Lee CC,Kastner DL,Torrelo A,Zlotogorski A,Moir S,Gadina M,McCoy P,Wesley R,Rother KI,Rother K,Hildebrand PW,Brogan P,Krüger E,Aksentijevich I,Goldbach-Mansky R

Proteomic characterization of the human sperm nucleus.
Proteomics11(13)2714-26(2011 Jul)
de Mateo S,Castillo J,Estanyol JM,Ballescà JL,Oliva R

MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis.
Immunology and cell biology88(8)851-6(2010 Nov-Dec)
Buschow SI,van Balkom BW,Aalberts M,Heck AJ,Wauben M,Stoorvogel W

PAC1 gene knockout reveals an essential role of chaperone-mediated 20S proteasome biogenesis and latent 20S proteasomes in cellular homeostasis.
Molecular and cellular biology30(15)3864-74(2010 Aug)
Sasaki K,Hamazaki J,Koike M,Hirano Y,Komatsu M,Uchiyama Y,Tanaka K,Murata S

REQUEST A QUOTE

EMAIL

gene@genscript.com

PHONE

1-877-436-7274

ONLINE FORM

Online Quote Submission

ONLINE FORM

Online Quote Submission

Psmb4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

News & Blogs

REQUEST A QUOTE

EMAIL

PHONE

ONLINE FORM

ONLINE FORM