Rgs12 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rgs12
Entrez Gene ID	54292
Full Name	regulator of G-protein signaling 12
General protein information	Preferred Names regulator of G-protein signaling 12 Names regulator of G-protein signaling 12 regulator of G-protein signalling 12
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 14 Map Location: 14q21
Summary	acts as a GTPase activating protein for Gi class alpha subunits; may play a role in desensitization and inhibition of G-protein coupled receptor signaling [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006251352.3	XP_006251414.1	regulator of G-protein signaling 12 isoform X1
NM_019339.1	NP_062212.1	regulator of G-protein signaling 12
XM_006251351.3	XP_006251413.1	regulator of G-protein signaling 12 isoform X1
XM_006251353.3	XP_006251415.1	regulator of G-protein signaling 12 isoform X1
XM_008770350.2	XP_008768572.1	regulator of G-protein signaling 12 isoform X1
XM_017599342.1	XP_017454831.1	regulator of G-protein signaling 12 isoform X2
XM_008770351.2	XP_008768573.1	regulator of G-protein signaling 12 isoform X3

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	LOC101731678	XP_004911316.1
Canis lupus familiaris (dog)	RGS12	XP_850554.1
Bos taurus (cattle)	RGS12	XP_005208281.1
Mus musculus (house mouse)	Rgs12	NP_775578.2
Homo sapiens (human)	RGS12	NP_937872.1
Rattus norvegicus (Norway rat)	Rgs12	NP_062212.1
Gallus gallus (chicken)	RGS12	XP_420820.3
Pan troglodytes (chimpanzee)	RGS12	XP_003310274.1
Macaca mulatta (Rhesus monkey)	RGS12	XP_002802183.1
Danio rerio (zebrafish)	rgs12b	NP_999889.1

Gene Ontology
Bibliography

Related articles in PubMed

Selective role for RGS12 as a Ras/Raf/MEK scaffold in nerve growth factor-mediated differentiation.
Willard MD, Willard FS, Li X, Cappell SD, Snider WD, Siderovski DP
The EMBO journal26(8)2029-40(2007 Apr)

Localization of the GoLoco motif carrier regulator of G-protein signalling 12 and 14 proteins in monkey and rat brain.
López-Aranda MF, Acevedo MJ, Carballo FJ, Gutiérrez A, Khan ZU
The European journal of neuroscience23(11)2971-82(2006 Jun)

Calcium-sensing receptor activation of rho involves filamin and rho-guanine nucleotide exchange factor.
Pi M, Spurney RF, Tu Q, Hinson T, Quarles LD
Endocrinology143(10)3830-8(2002 Oct)

RGS12 and RGS14 GoLoco motifs are G alpha(i) interaction sites with guanine nucleotide dissociation inhibitor Activity.
Kimple RJ, De Vries L, Tronchère H, Behe CI, Morris RA, Gist Farquhar M, Siderovski DP
The Journal of biological chemistry276(31)29275-81(2001 Aug)

Tyrosine-kinase-dependent recruitment of RGS12 to the N-type calcium channel.
Schiff ML, Siderovski DP, Jordan JD, Brothers G, Snow B, De Vries L, Ortiz DF, Diversé-Pierluissi M
Nature408(6813)723-7(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Rgs12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rgs12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa43420	XM_008770351.2 Latest version!	Rattus norvegicus regulator of G-protein signaling 12 (Rgs12), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ORa58403	XM_017599342.1 Latest version!	Rattus norvegicus regulator of G-protein signaling 12 (Rgs12), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ORa04476	NM_019339.1 Latest version!	Rattus norvegicus regulator of G-protein signaling 12 (Rgs12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ORa36328	XM_008770350.2 Latest version!	Rattus norvegicus regulator of G-protein signaling 12 (Rgs12), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ORa36328	XM_006251353.3 Latest version!	Rattus norvegicus regulator of G-protein signaling 12 (Rgs12), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ORa36328	XM_006251352.3 Latest version!	Rattus norvegicus regulator of G-protein signaling 12 (Rgs12), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ORa36328	XM_006251351.3 Latest version!	Rattus norvegicus regulator of G-protein signaling 12 (Rgs12), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rgs12 ( XM_008770351.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008768573.1 Rattus norvegicus regulator of G-protein signaling 12 (Rgs12), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ORa43420
Clone ID Related Accession (Same CDS sequence)	XM_008770351.2
Accession Version	XM_008770351.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2430bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	regulator of G-protein signaling 12 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008770351.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTTGG AAAAGGGGCT GTCAGATGAT TCGGACGTTT TCATTGACCA GCAGTCTGCA 
ACTGTGTCTG ATGGTGAGTT GACGGGAGCT GACCTGAAGG ACTGCATCAG CAACAACAGC 
CTGAGCAGCA ATGCCAGCCT CCCCAGTGTG CAGAGCTGCC GGCGCCTAAG AGAGAGGAGA 
GTTGCAAGCT GGGCAGTGTC ATTTGAGCGT CTGCTGCAGG ATCCTGTCGG CGTCCGCTAC 
TTCTCCGATT TTCTACGGAA GGAATTCAGT GAAGAGAACA TTTTATTCTG GCAGGCCTGT 
GAATGTTTCA GTCATGTCCC TGCACATGAC AAAAAAGAGC TCTCTTACAG GGCCCGGGAG 
ATTTTCAGTA AATTCCTGTG CAGCAAAGCC ACCACTCCTG TCAACATTGA CAGCCAGGCC 
CAGCTGGCAG ACGATATTCT CAATGCACCC CACCCCGACA TGTTCAAGGA ACAGCAGCTC 
CAGATTTTCA ACCTGATGAA GTTTGACAGC TATACTCGAT TTCTGAAATC CCAGCTGTAC 
CAAGAATGCG TCCTGGCAGA GGTGGAGGGG CGTACCCTCC CGGACTCCCA GCAGGTCCCC 
AGCAGCCCAG CTTCCAAGCA CAGCATCAGC TCTGACCACT CCAATGTATC CACACCGAAA 
AAGTTAAGTG GAAAATCAAA ATCAGGACGA TCCCTGAATG AAGATGTTGG GGAAGAGGAT 
AGTGAGAAAA AGCGCAAAGG GGCCTTTTTC TCTTGGTCAA GGAGCAGGAG CACGGGGCGA 
TCCCAGAAGA AGAAGGACCA TGGTGATCAT GCCCATGATG CCCTTCATGC CAATGGAGGA 
CTCTGCCGTC GGGAGTCCCA AGGCTCTGTC TCTTCTGCGG GGAGCCTAGA CCTGTCAGAG 
GCCTGTAGGA CCTCAGCACT CGAGAGAGAT AAGGCTGCCA AACACTGCTG TGTCCACCTC 
CCAGACGGGA CATCCTGTGT GGTTGCTGTC AAGTCCGGGT TTTCCATCAA AGAGATCCTG 
TCTGGATTGT GTGAACGGCA TGGCATCAAT GGGGCTGCTG TGGACCTCTT CCTGGTGGGC 
GGAGACAAGC CTCTGGTGCT GCATCAGGAC AGCAGCATCC TGGCCACCAG GGACCTACGG 
TTGGAAAAGC GTACTTTGTT TCGGCTGGAT CTCGTTCCAA TTAACCGGTC AGTGGGACTC 
AAGGCCAAGC CCACCAAGCC AGTCACGGAA GTGTTGCGGC CCGTGGTGGC CAAGTACGGC 
CTGGATCTGG GTAGCCTGCT GGTGAGGCTG AGTGGGGAGA AGGAGCCCCT GGACCTTGGT 
GCCCCTATAT CAAGTCTGGA TGGACAGCGG GTCATCCTAG AGGAGAGGGA TCCTTCTAGA 
GGGAAAGTGT CCACAGAGAA ACAGAAAGGT GCTCCTGTCA AACAAAGCTC AGCTGTGAAC 
TCCAGCCCCA GAAACCACTC GGCTATGGGA GAGGAGAGAA CACTAGGCAA GTCTAACTCT 
ATTAAAATAA GAGGAGAAAA TGGAAAAAGT GCTAGGGATC CCCGGCTTTC AAAGAGAGAA 
GAATCTATTG CAAAGATTGG GAAAAAAAAA TATCAGAAAA TTAATTTGGA CGAAGCAGAG 
GAGTTTTTTG AGCTCATTTC CAAAGCTCAG AGCAACAGAG CCGATGACCA GCGTGGGCTG 
CTAAGAAAGG AAGACCTGGT ACTGCCTGAG TTTCTTCGCT TACCTCCTGG TTCCTCAGAA 
CTTGCCCTGT CCAGCCCACC CCCAGTCAAG GGCTTTAGCA AGAGAGCGGT TACAAGCCAT 
GGCCAGGAGG GAGCAGTTCA GACAGAGGAG AGCTACAGCG ACAGCCCAGC TACCAGCCCT 
GCCTCAGCCC AGAGCCCCTG CTCAGCCTAC AGCCCTGGCT CCGCCCACAG CCCCGGCTCT 
GCCCACAGCC CTGGCTCCGC CCACAGCACC CCTGGGCCTC CTGGGACCGC CCAACCTGGA 
GAGAAGCCTA CAAAGCCCTC CTGCATCTCC ACAGTTCAAG AGGGCACTAC ACAGGCCTGG 
AGGAGGCTAT CACCAGAACT GGAGGCTGGG GGCATCCAGA CTGTGGAGGA GGAGCAGGTG 
GCTGACCTGA CCCTGATGGG GGAGGGGGAC ATCAGCAGCC CCAACAGCAC GCTGCTACCA 
CCACCACCCC TTCCCCAAGA CACACCAGGA CCTACAAGAC CAGGGGGCAG GGCCCGAGGC 
AGCGAAGGCC TCCCAGTCAA CAGGATCATC GACGTGGACC TCGTTACGGG TGTGCCTCCC 
GGGTGGGGAG GCAGCATTCT GGGGGTGCAG CTGGGTCCCA GGAGGGTGAA AGCCAATGGA 
CCCTTCTTAC CAAACGGTCC TGGCCAGAGC ACCATCCCAG GTGAGCCCAC CAAGCCCAAG 
GCCAGCACAC ACCATGCCAC CTTTGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008768573.1
CDS	487..2916
Translation	MNLEKGLSDDSDVFIDQQSATVSDGELTGADLKDCISNNSLSSNASLPSVQSCRRLRERRVASWAVSFERLLQDPVGVRYFSDFLRKEFSEENILFWQACECFSHVPAHDKKELSYRAREIFSKFLCSKATTPVNIDSQAQLADDILNAPHPDMFKEQQLQIFNLMKFDSYTRFLKSQLYQECVLAEVEGRTLPDSQQVPSSPASKHSISSDHSNVSTPKKLSGKSKSGRSLNEDVGEEDSEKKRKGAFFSWSRSRSTGRSQKKKDHGDHAHDALHANGGLCRRESQGSVSSAGSLDLSEACRTSALERDKAAKHCCVHLPDGTSCVVAVKSGFSIKEILSGLCERHGINGAAVDLFLVGGDKPLVLHQDSSILATRDLRLEKRTLFRLDLVPINRSVGLKAKPTKPVTEVLRPVVAKYGLDLGSLLVRLSGEKEPLDLGAPISSLDGQRVILEERDPSRGKVSTEKQKGAPVKQSSAVNSSPRNHSAMGEERTLGKSNSIKIRGENGKSARDPRLSKREESIAKIGKKKYQKINLDEAEEFFELISKAQSNRADDQRGLLRKEDLVLPEFLRLPPGSSELALSSPPPVKGFSKRAVTSHGQEGAVQTEESYSDSPATSPASAQSPCSAYSPGSAHSPGSAHSPGSAHSTPGPPGTAQPGEKPTKPSCISTVQEGTTQAWRRLSPELEAGGIQTVEEEQVADLTLMGEGDISSPNSTLLPPPPLPQDTPGPTRPGGRARGSEGLPVNRIIDVDLVTGVPPGWGGSILGVQLGPRRVKANGPFLPNGPGQSTIPGEPTKPKASTHHATFV

Target ORF information:

RefSeq Version	XM_008770351.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus regulator of G-protein signaling 12 (Rgs12), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008770351.2

ORF Insert Sequence:

ATGAATTTGG AAAAGGGGCT GTCAGATGAT TCGGACGTTT TCATTGACCA GCAGTCTGCA 
ACTGTGTCTG ATGGTGAGTT GACGGGAGCT GACCTGAAGG ACTGCATCAG CAACAACAGC 
CTGAGCAGCA ATGCCAGCCT CCCCAGTGTG CAGAGCTGCC GGCGCCTAAG AGAGAGGAGA 
GTTGCAAGCT GGGCAGTGTC ATTTGAGCGT CTGCTGCAGG ATCCTGTCGG CGTCCGCTAC 
TTCTCCGATT TTCTACGGAA GGAATTCAGT GAAGAGAACA TTTTATTCTG GCAGGCCTGT 
GAATGTTTCA GTCATGTCCC TGCACATGAC AAAAAAGAGC TCTCTTACAG GGCCCGGGAG 
ATTTTCAGTA AATTCCTGTG CAGCAAAGCC ACCACTCCTG TCAACATTGA CAGCCAGGCC 
CAGCTGGCAG ACGATATTCT CAATGCACCC CACCCCGACA TGTTCAAGGA ACAGCAGCTC 
CAGATTTTCA ACCTGATGAA GTTTGACAGC TATACTCGAT TTCTGAAATC CCAGCTGTAC 
CAAGAATGCG TCCTGGCAGA GGTGGAGGGG CGTACCCTCC CGGACTCCCA GCAGGTCCCC 
AGCAGCCCAG CTTCCAAGCA CAGCATCAGC TCTGACCACT CCAATGTATC CACACCGAAA 
AAGTTAAGTG GAAAATCAAA ATCAGGACGA TCCCTGAATG AAGATGTTGG GGAAGAGGAT 
AGTGAGAAAA AGCGCAAAGG GGCCTTTTTC TCTTGGTCAA GGAGCAGGAG CACGGGGCGA 
TCCCAGAAGA AGAAGGACCA TGGTGATCAT GCCCATGATG CCCTTCATGC CAATGGAGGA 
CTCTGCCGTC GGGAGTCCCA AGGCTCTGTC TCTTCTGCGG GGAGCCTAGA CCTGTCAGAG 
GCCTGTAGGA CCTCAGCACT CGAGAGAGAT AAGGCTGCCA AACACTGCTG TGTCCACCTC 
CCAGACGGGA CATCCTGTGT GGTTGCTGTC AAGTCCGGGT TTTCCATCAA AGAGATCCTG 
TCTGGATTGT GTGAACGGCA TGGCATCAAT GGGGCTGCTG TGGACCTCTT CCTGGTGGGC 
GGAGACAAGC CTCTGGTGCT GCATCAGGAC AGCAGCATCC TGGCCACCAG GGACCTACGG 
TTGGAAAAGC GTACTTTGTT TCGGCTGGAT CTCGTTCCAA TTAACCGGTC AGTGGGACTC 
AAGGCCAAGC CCACCAAGCC AGTCACGGAA GTGTTGCGGC CCGTGGTGGC CAAGTACGGC 
CTGGATCTGG GTAGCCTGCT GGTGAGGCTG AGTGGGGAGA AGGAGCCCCT GGACCTTGGT 
GCCCCTATAT CAAGTCTGGA TGGACAGCGG GTCATCCTAG AGGAGAGGGA TCCTTCTAGA 
GGGAAAGTGT CCACAGAGAA ACAGAAAGGT GCTCCTGTCA AACAAAGCTC AGCTGTGAAC 
TCCAGCCCCA GAAACCACTC GGCTATGGGA GAGGAGAGAA CACTAGGCAA GTCTAACTCT 
ATTAAAATAA GAGGAGAAAA TGGAAAAAGT GCTAGGGATC CCCGGCTTTC AAAGAGAGAA 
GAATCTATTG CAAAGATTGG GAAAAAAAAA TATCAGAAAA TTAATTTGGA CGAAGCAGAG 
GAGTTTTTTG AGCTCATTTC CAAAGCTCAG AGCAACAGAG CCGATGACCA GCGTGGGCTG 
CTAAGAAAGG AAGACCTGGT ACTGCCTGAG TTTCTTCGCT TACCTCCTGG TTCCTCAGAA 
CTTGCCCTGT CCAGCCCACC CCCAGTCAAG GGCTTTAGCA AGAGAGCGGT TACAAGCCAT 
GGCCAGGAGG GAGCAGTTCA GACAGAGGAG AGCTACAGCG ACAGCCCAGC TACCAGCCCT 
GCCTCAGCCC AGAGCCCCTG CTCAGCCTAC AGCCCTGGCT CCGCCCACAG CCCCGGCTCT 
GCCCACAGCC CTGGCTCCGC CCACAGCACC CCTGGGCCTC CTGGGACCGC CCAACCTGGA 
GAGAAGCCTA CAAAGCCCTC CTGCATCTCC ACAGTTCAAG AGGGCACTAC ACAGGCCTGG 
AGGAGGCTAT CACCAGAACT GGAGGCTGGG GGCATCCAGA CTGTGGAGGA GGAGCAGGTG 
GCTGACCTGA CCCTGATGGG GGAGGGGGAC ATCAGCAGCC CCAACAGCAC GCTGCTACCA 
CCACCACCCC TTCCCCAAGA CACACCAGGA CCTACAAGAC CAGGGGGCAG GGCCCGAGGC 
AGCGAAGGCC TCCCAGTCAA CAGGATCATC GACGTGGACC TCGTTACGGG TGTGCCTCCC 
GGGTGGGGAG GCAGCATTCT GGGGGTGCAG CTGGGTCCCA GGAGGGTGAA AGCCAATGGA 
CCCTTCTTAC CAAACGGTCC TGGCCAGAGC ACCATCCCAG GTGAGCCCAC CAAGCCCAAG 
GCCAGCACAC ACCATGCCAC CTTTGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rgs12 ( XM_017599342.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017454831.1 Rattus norvegicus regulator of G-protein signaling 12 (Rgs12), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ORa58403
Clone ID Related Accession (Same CDS sequence)	XM_017599342.1
Accession Version	XM_017599342.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2442bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	regulator of G-protein signaling 12 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCAG AAGGGTGGTT TACGGTGAAT GGTTGGAAGT ATTTGGTTAG ATGGCAGCAC 
CACAGTGGCT CTTATCAGCT CTACCCAGAG TTGACGGGAG CTGACCTGAA GGACTGCATC 
AGCAACAACA GCCTGAGCAG CAATGCCAGC CTCCCCAGTG TGCAGAGCTG CCGGCGCCTA 
AGAGAGAGGA GAGTTGCAAG CTGGGCAGTG TCATTTGAGC GTCTGCTGCA GGATCCTGTC 
GGCGTCCGCT ACTTCTCCGA TTTTCTACGG AAGGAATTCA GTGAAGAGAA CATTTTATTC 
TGGCAGGCCT GTGAATGTTT CAGTCATGTC CCTGCACATG ACAAAAAAGA GCTCTCTTAC 
AGGGCCCGGG AGATTTTCAG TAAATTCCTG TGCAGCAAAG CCACCACTCC TGTCAACATT 
GACAGCCAGG CCCAGCTGGC AGACGATATT CTCAATGCAC CCCACCCCGA CATGTTCAAG 
GAACAGCAGC TCCAGATTTT CAACCTGATG AAGTTTGACA GCTATACTCG ATTTCTGAAA 
TCCCAGCTGT ACCAAGAATG CGTCCTGGCA GAGGTGGAGG GGCGTACCCT CCCGGACTCC 
CAGCAGGTCC CCAGCAGCCC AGCTTCCAAG CACAGCATCA GCTCTGACCA CTCCAATGTA 
TCCACACCGA AAAAGTTAAG TGGAAAATCA AAATCAGGAC GATCCCTGAA TGAAGATGTT 
GGGGAAGAGG ATAGTGAGAA AAAGCGCAAA GGGGCCTTTT TCTCTTGGTC AAGGAGCAGG 
AGCACGGGGC GATCCCAGAA GAAGAAGGAC CATGGTGATC ATGCCCATGA TGCCCTTCAT 
GCCAATGGAG GACTCTGCCG TCGGGAGTCC CAAGGCTCTG TCTCTTCTGC GGGGAGCCTA 
GACCTGTCAG AGGCCTGTAG GACCTCAGCA CTCGAGAGAG ATAAGGCTGC CAAACACTGC 
TGTGTCCACC TCCCAGACGG GACATCCTGT GTGGTTGCTG TCAAGTCCGG GTTTTCCATC 
AAAGAGATCC TGTCTGGATT GTGTGAACGG CATGGCATCA ATGGGGCTGC TGTGGACCTC 
TTCCTGGTGG GCGGAGACAA GCCTCTGGTG CTGCATCAGG ACAGCAGCAT CCTGGCCACC 
AGGGACCTAC GGTTGGAAAA GCGTACTTTG TTTCGGCTGG ATCTCGTTCC AATTAACCGG 
TCAGTGGGAC TCAAGGCCAA GCCCACCAAG CCAGTCACGG AAGTGTTGCG GCCCGTGGTG 
GCCAAGTACG GCCTGGATCT GGGTAGCCTG CTGGTGAGGC TGAGTGGGGA GAAGGAGCCC 
CTGGACCTTG GTGCCCCTAT ATCAAGTCTG GATGGACAGC GGGTCATCCT AGAGGAGAGG 
GATCCTTCTA GAGGGAAAGT GTCCACAGAG AAACAGAAAG GTGCTCCTGT CAAACAAAGC 
TCAGCTGTGA ACTCCAGCCC CAGAAACCAC TCGGCTATGG GAGAGGAGAG AACACTAGGC 
AAGTCTAACT CTATTAAAAT AAGAGGAGAA AATGGAAAAA GTGCTAGGGA TCCCCGGCTT 
TCAAAGAGAG AAGAATCTAT TGCAAAGATT GGGAAAAAAA AATATCAGAA AATTAATTTG 
GACGAAGCAG AGGAGTTTTT TGAGCTCATT TCCAAAGCTC AGAGCAACAG AGCCGATGAC 
CAGCGTGGGC TGCTAAGAAA GGAAGACCTG GTACTGCCTG AGTTTCTTCG CTTACCTCCT 
GGTTCCTCAG AACTTGCCCT GTCCAGCCCA CCCCCAGTCA AGGGCTTTAG CAAGAGAGCG 
GTTACAAGCC ATGGCCAGGA GGGAGCAGTT CAGACAGAGG AGAGCTACAG CGACAGCCCA 
GCTACCAGCC CTGCCTCAGC CCAGAGCCCC TGCTCAGCCT ACAGCCCTGG CTCCGCCCAC 
AGCCCCGGCT CTGCCCACAG CCCTGGCTCC GCCCACAGCA CCCCTGGGCC TCCTGGGACC 
GCCCAACCTG GAGAGAAGCC TACAAAGCCC TCCTGCATCT CCACAGTTCA AGAGGGCACT 
ACACAGGCCT GGAGGAGGCT ATCACCAGAA CTGGAGGCTG GGGGCATCCA GACTGTGGAG 
GAGGAGCAGG TGGCTGACCT GACCCTGATG GGGGAGGGGG ACATCAGCAG CCCCAACAGC 
ACGCTGCTAC CACCACCACC CCTTCCCCAA GACACACCAG GACCTACAAG ACCAGGGGGC 
AGGGCCCGAG GCAGCGAAGG CCTCCCAGTC AACAGGATCA TCGACGTGGA CCTCGTTACG 
GGTGTGCCTC CCGGGTGGGG AGGCAGCATT CTGGGGGTGC AGCTGGGTCC CAGGAGGGTG 
AAAGCCAATG GACCCTTCTT ACCAAACGGT CCTGGCCAGA GCACCATCCC AGGTGAGCCC 
ACCAAGCCCA AGGCCAGCAC ACACCATGCC ACCTTTGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017454831.1
CDS	279..2720
Translation	MDSEGWFTVNGWKYLVRWQHHSGSYQLYPELTGADLKDCISNNSLSSNASLPSVQSCRRLRERRVASWAVSFERLLQDPVGVRYFSDFLRKEFSEENILFWQACECFSHVPAHDKKELSYRAREIFSKFLCSKATTPVNIDSQAQLADDILNAPHPDMFKEQQLQIFNLMKFDSYTRFLKSQLYQECVLAEVEGRTLPDSQQVPSSPASKHSISSDHSNVSTPKKLSGKSKSGRSLNEDVGEEDSEKKRKGAFFSWSRSRSTGRSQKKKDHGDHAHDALHANGGLCRRESQGSVSSAGSLDLSEACRTSALERDKAAKHCCVHLPDGTSCVVAVKSGFSIKEILSGLCERHGINGAAVDLFLVGGDKPLVLHQDSSILATRDLRLEKRTLFRLDLVPINRSVGLKAKPTKPVTEVLRPVVAKYGLDLGSLLVRLSGEKEPLDLGAPISSLDGQRVILEERDPSRGKVSTEKQKGAPVKQSSAVNSSPRNHSAMGEERTLGKSNSIKIRGENGKSARDPRLSKREESIAKIGKKKYQKINLDEAEEFFELISKAQSNRADDQRGLLRKEDLVLPEFLRLPPGSSELALSSPPPVKGFSKRAVTSHGQEGAVQTEESYSDSPATSPASAQSPCSAYSPGSAHSPGSAHSPGSAHSTPGPPGTAQPGEKPTKPSCISTVQEGTTQAWRRLSPELEAGGIQTVEEEQVADLTLMGEGDISSPNSTLLPPPPLPQDTPGPTRPGGRARGSEGLPVNRIIDVDLVTGVPPGWGGSILGVQLGPRRVKANGPFLPNGPGQSTIPGEPTKPKASTHHATFV

Target ORF information:

RefSeq Version	XM_017599342.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus regulator of G-protein signaling 12 (Rgs12), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017599342.1

ORF Insert Sequence:

ATGGACTCAG AAGGGTGGTT TACGGTGAAT GGTTGGAAGT ATTTGGTTAG ATGGCAGCAC 
CACAGTGGCT CTTATCAGCT CTACCCAGAG TTGACGGGAG CTGACCTGAA GGACTGCATC 
AGCAACAACA GCCTGAGCAG CAATGCCAGC CTCCCCAGTG TGCAGAGCTG CCGGCGCCTA 
AGAGAGAGGA GAGTTGCAAG CTGGGCAGTG TCATTTGAGC GTCTGCTGCA GGATCCTGTC 
GGCGTCCGCT ACTTCTCCGA TTTTCTACGG AAGGAATTCA GTGAAGAGAA CATTTTATTC 
TGGCAGGCCT GTGAATGTTT CAGTCATGTC CCTGCACATG ACAAAAAAGA GCTCTCTTAC 
AGGGCCCGGG AGATTTTCAG TAAATTCCTG TGCAGCAAAG CCACCACTCC TGTCAACATT 
GACAGCCAGG CCCAGCTGGC AGACGATATT CTCAATGCAC CCCACCCCGA CATGTTCAAG 
GAACAGCAGC TCCAGATTTT CAACCTGATG AAGTTTGACA GCTATACTCG ATTTCTGAAA 
TCCCAGCTGT ACCAAGAATG CGTCCTGGCA GAGGTGGAGG GGCGTACCCT CCCGGACTCC 
CAGCAGGTCC CCAGCAGCCC AGCTTCCAAG CACAGCATCA GCTCTGACCA CTCCAATGTA 
TCCACACCGA AAAAGTTAAG TGGAAAATCA AAATCAGGAC GATCCCTGAA TGAAGATGTT 
GGGGAAGAGG ATAGTGAGAA AAAGCGCAAA GGGGCCTTTT TCTCTTGGTC AAGGAGCAGG 
AGCACGGGGC GATCCCAGAA GAAGAAGGAC CATGGTGATC ATGCCCATGA TGCCCTTCAT 
GCCAATGGAG GACTCTGCCG TCGGGAGTCC CAAGGCTCTG TCTCTTCTGC GGGGAGCCTA 
GACCTGTCAG AGGCCTGTAG GACCTCAGCA CTCGAGAGAG ATAAGGCTGC CAAACACTGC 
TGTGTCCACC TCCCAGACGG GACATCCTGT GTGGTTGCTG TCAAGTCCGG GTTTTCCATC 
AAAGAGATCC TGTCTGGATT GTGTGAACGG CATGGCATCA ATGGGGCTGC TGTGGACCTC 
TTCCTGGTGG GCGGAGACAA GCCTCTGGTG CTGCATCAGG ACAGCAGCAT CCTGGCCACC 
AGGGACCTAC GGTTGGAAAA GCGTACTTTG TTTCGGCTGG ATCTCGTTCC AATTAACCGG 
TCAGTGGGAC TCAAGGCCAA GCCCACCAAG CCAGTCACGG AAGTGTTGCG GCCCGTGGTG 
GCCAAGTACG GCCTGGATCT GGGTAGCCTG CTGGTGAGGC TGAGTGGGGA GAAGGAGCCC 
CTGGACCTTG GTGCCCCTAT ATCAAGTCTG GATGGACAGC GGGTCATCCT AGAGGAGAGG 
GATCCTTCTA GAGGGAAAGT GTCCACAGAG AAACAGAAAG GTGCTCCTGT CAAACAAAGC 
TCAGCTGTGA ACTCCAGCCC CAGAAACCAC TCGGCTATGG GAGAGGAGAG AACACTAGGC 
AAGTCTAACT CTATTAAAAT AAGAGGAGAA AATGGAAAAA GTGCTAGGGA TCCCCGGCTT 
TCAAAGAGAG AAGAATCTAT TGCAAAGATT GGGAAAAAAA AATATCAGAA AATTAATTTG 
GACGAAGCAG AGGAGTTTTT TGAGCTCATT TCCAAAGCTC AGAGCAACAG AGCCGATGAC 
CAGCGTGGGC TGCTAAGAAA GGAAGACCTG GTACTGCCTG AGTTTCTTCG CTTACCTCCT 
GGTTCCTCAG AACTTGCCCT GTCCAGCCCA CCCCCAGTCA AGGGCTTTAG CAAGAGAGCG 
GTTACAAGCC ATGGCCAGGA GGGAGCAGTT CAGACAGAGG AGAGCTACAG CGACAGCCCA 
GCTACCAGCC CTGCCTCAGC CCAGAGCCCC TGCTCAGCCT ACAGCCCTGG CTCCGCCCAC 
AGCCCCGGCT CTGCCCACAG CCCTGGCTCC GCCCACAGCA CCCCTGGGCC TCCTGGGACC 
GCCCAACCTG GAGAGAAGCC TACAAAGCCC TCCTGCATCT CCACAGTTCA AGAGGGCACT 
ACACAGGCCT GGAGGAGGCT ATCACCAGAA CTGGAGGCTG GGGGCATCCA GACTGTGGAG 
GAGGAGCAGG TGGCTGACCT GACCCTGATG GGGGAGGGGG ACATCAGCAG CCCCAACAGC 
ACGCTGCTAC CACCACCACC CCTTCCCCAA GACACACCAG GACCTACAAG ACCAGGGGGC 
AGGGCCCGAG GCAGCGAAGG CCTCCCAGTC AACAGGATCA TCGACGTGGA CCTCGTTACG 
GGTGTGCCTC CCGGGTGGGG AGGCAGCATT CTGGGGGTGC AGCTGGGTCC CAGGAGGGTG 
AAAGCCAATG GACCCTTCTT ACCAAACGGT CCTGGCCAGA GCACCATCCC AGGTGAGCCC 
ACCAAGCCCA AGGCCAGCAC ACACCATGCC ACCTTTGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rgs12 ( NM_019339.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_062212.1 Rattus norvegicus regulator of G-protein signaling 12 (Rgs12), mRNA.

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CloneID	ORa04476
Clone ID Related Accession (Same CDS sequence)	NM_019339.1
Accession Version	NM_019339.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4164bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577203200000
Organism	Rattus norvegicus(Norway rat)
Product	regulator of G-protein signaling 12
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U92280.1. ##Evidence-Data-START## Transcript exon combination :: U92280.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGTACAGGG CTGGGGAGCC AGGGAAGCGG CAGTCTGGCC CTGCGCCCCC TCGGGTGCGG 
AGTGTGGAAG TGGCCCGAGG CAGAGCTGGC TACGGCTTCA CCCTGTCAGG ACAGGCACCC 
TGTGTGCTCA GCTGCGTCAT GAGGGGCAGC CCTGCAGATT TCGTGGGCCT CCGGGCTGGG 
GACCAGATAC TTGCCATCAA CGAAATCAAT GTGAAGAAAG CCTCACATGA AGATGTGGTG 
AAACTCATCG GGAAATGCTC TGGAGTGCTG CGCATGGTGA TCTCCGAGGG CAGCAGCCAT 
GTGGAGCCCT CTTCCAGCGA TGAAGAAGGC GGCCTGTGTG AGGGGAAAGG CTGGCTGAGG 
CCGAAACTTG ACTCTAAGGC TTTGGGTATA AACAGGGCAG AGAGGGTTGT GGAAGAGGTG 
CAGTCTGGTG GGATTTTCAA CATGATCTTT GAAAGCCCAA GTCTTTGTGC CAGTGGCTCT 
GAGCCCCTGA AACTGAAGCA GAGGTCCTTG TCAGAGTCAG CTGCTTTGCG GCTTGATGTT 
GGCCAGGACA GTCTCTGTAC ACCACATCCC AGCATGCTTT CCAAGGAAGA GATATCAAAG 
GTTATCAATG ACGATTCTGT GTTCACTGTA GGGCTGGACA ATCACGATGA CTTTGGACTA 
GATGCAAGTA TTTTAAATGT TGCCATGGTT GTGGGCTATT TGGGCTCCAT CGAGCTCCCT 
TCCACAAGTT CCAACCTGGA GCATGACAGT TTACAGGCTA TTCGTGGCTG CATGCGCCGC 
CTGCGGGCTG AGCAGAAGAT CCACTCACTA GTGACCATGA AGGTCATGCA CGACTGTGTG 
CAGCTGGTCA CTGACAGGGC TGGTGTGGTG GCAGAGTACC CTGCCGAGAA GCTGGCGTTC 
AGTGCAGTGT GCCCAGACGA CAGGCGGTTC TTCGGGCTGG TGACCATGCA GACCAATGAT 
GATGGGTGCT TGGCACAGGA AGATGAAGGT GCCCTGAGGA CATCCTGCCA TGTCTTCATG 
GTGGACCCAG ACTTATTTCA TCACAAGATC CACCAAGGCA TCGCGAGAAG ATTTGGATTT 
GCATGCACGG CAGATCCAGA CACGAGTGGC TGTTTGGAAT TCCCAGCATC TTCCCTCCCT 
GTGCTTCAGT TCATCTCCGT TCTGTACCGA GACATGGGTG AACTGATTGA GGGTGTGCGG 
GCCCGTGCCT TTCTAGATGG GGATGCCGAT GCCCACCAGA ATAATAGCAC CAGCAGCAAC 
AGTGACAGCG GCATTGGCAA TTTCAACCAG GAAGAGAAGA GCAACAGGGT GCTTGTAGTT 
GACCTGGGTG GGGGCTCGAG CAGGCATGGC CAGGGCAGTA GTCCGGGGTG GGAGAGTGTA 
AGTGGAAGAG GCTCACAGCC TTGGAGTGCA CCCTGGAATG GAACTTTCTG CCACGACTCA 
GAGGCGGGCT CCCCACTGGA AACCAGCCCC AACACTGACA GGTTCTGGGA CTTAACAAAG 
CATTCAGGGC CTGTTTTTCA CATGGAGGTC CCTCCAGCCA CCTTGAGGAG TTCTATACCC 
CCTTCCAAGA GGGGTGCTAC AGGTTCCAGC TGTGGTTTCA ACCAGAGGTG GCTCCCTGTC 
CATGTGCTAC AAGAGTGGCA GTGTGGACAT GCCAGTGACC AGGAGTCTTA CACAGACTCT 
ACGGACGGGT GGTCCAGTGT GAACTGTGGC ACACTGCCCC CTCCCATGAG CAAGATTCCT 
GCAGACCGAT ACCGGGTGGA GGGCAGCTTT GCTCAGGCCC CACTGAGTAC CCAGAAGAGG 
GACTGGTCTC GGAAGGCTTT CGGAATGCAA AACCTATTTG GACCCCATCG AAATGTTAGA 
AAGACCAAAG AGGACAAAAA GAGTTCCAAA TTGGGGCGGG GAGTTGCCCT GGCCCAGACT 
TCTCAGCGCA CTTCAGCTCG CAGATCCTTT GGACGATCTA GGAGATTCAG CCTCACGCGC 
TCCCTTGATG ATCTCGAGTC TGCAACTGTG TCTGATGGTG AGTTGACGGG AGCTGACCTG 
AAGGACTGCA TCAGCAACAA CAGCCTGAGC AGCAATGCCA GCCTCCCCAG TGTGCAGAGC 
TGCCGGCGCC TAAGAGAGAG GAGAGTTGCA AGCTGGGCAG TGTCATTTGA GCGTCTGCTG 
CAGGATCCTG TCGGCGTCCG CTACTTCTCC GATTTTCTAC GGAAGGAATT CAGTGAAGAG 
AACATTTTAT TCTGGCAGGC CTGTGAATGT TTCAGTCATG TCCCTGCACA TGACAAAAAA 
GAGCTCTCTT ACAGGGCCCG GGAGATTTTC AGTAAATTCC TGTGCAGCAA AGCCACCACT 
CCTGTCAACA TTGACAGCCA GGCCCAGCTG GCAGACGATA TTCTCAATGC ACCCCACCCC 
GACATGTTCA AGGAACAGCA GCTCCAGATT TTCAACCTGA TGAAGTTTGA CAGCTATACT 
CGATTTCTGA AATCCCAGCT GTACCAAGAA TGCGTCCTGG CAGAGGTGGA GGGGCGTACC 
CTCCCGGACT CCCAGCAGGT CCCCAGCAGC CCAGCTTCCA AGCACAGCAT CAGCTCTGAC 
CACTCCAATG TATCCACACC GAAAAAGTTA AGTGGAAAAT CAAAATCAGG ACGATCCCTG 
AATGAAGATG TTGGGGAAGA GGATAGTGAG AAAAAGCGCA AAGGGGCCTT TTTCTCTTGG 
TCAAGGAGCA GGAGCACGGG GCGATCCCAG AAGAAGAAGG ACCATGGTGA TCATGCCCAT 
GATGCCCTTC ATGCCAATGG AGGACTCTGC CGTCGGGAGT CCCAAGGCTC TGTCTCTTCT 
GCGGGGAGCC TAGACCTGTC AGAGGCCTGT AGGACCTCAG CACTCGAGAG AGATAAGGCT 
GCCAAACACT GCTGTGTCCA CCTCCCAGAC GGGACATCCT GTGTGGTTGC TGTCAAGTCC 
GGGTTTTCCA TCAAAGAGAT CCTGTCTGGA TTGTGTGAAC GGCATGGCAT CAATGGGGCT 
GCTGTGGACC TCTTCCTGGT GGGCGGAGAC AAGCCTCTGG TGCTGCATCA GGACAGCAGC 
ATCCTGGCCA CCAGGGACCT ACGGTTGGGA AAGCGTACTT TGTTTCGGCT GGATCTCGTT 
CCAATTAACC GGTCAGTGGG ACTCAAGGCC AAGCCCACCA AGCCAGTCAC GGAAGTGTTG 
CGGCCCGTGG TGGCCAAGTA CGGCCTGGAT CTGGGTAGCC TGCTGGTGAG GCTGAGTGGG 
GAGAAGGAGC CCCTGGACCT TGGTGCCCCT ATATCAAGTC TGGATGGACA GCGGGTCATC 
CTAGAGGAGA GGGATCCTTC TAGAGGGAAA GTGTCCACAG AGAAACAGAA AGGTGCTCCT 
GTCAAACAAA GCTCAGCTGT GAACTCCAGC CCCAGAAACC ACTCGGCTAT GGGAGAGGAG 
AGAACACTAG GCAAGTCTAA CTCTATTAAA ATAAGAGGAG AAAATGGAAA AAGTGCTAGG 
GATCCCCGGC TTTCAAAGAG AGAAGAATCT ATTGCAAAGA TTGGGAAAAA AAAATATCAG 
AAAATTAATT TGGACGAAGC AGAGGAGTTT TTTGAGCTCA TTTCCAAAGC TCAGAGCAAC 
AGAGCCGATG ACCAGCGTGG GCTGCTAAGA AAGGAAGACC TGGTACTGCC TGAGTTTCTT 
CGCTTACCTC CTGGTTCCTC AGAACTTGCC CTGTCCAGCC CACCCCCAGT CAAGGGCTTT 
AGCAAGAGAG CGGTTACAAG CCATGGCCAG GAGGGAGCAG TTCAGACAGA GGAGAGCTAC 
AGCGACAGCC CAGCTACCAG CCCTGCCTCA GCCCAGAGCC CCTGCTCAGC CTACAGCCCT 
GGCTCCGCCC ACAGCCCCGG CTCTGCCCAC AGCCCTGGCT CCGCCCACAG CACCCCTGGG 
CCTCCTGGGA CCGCCCAACC TGGAGAGAAG CCTACAAAGC CCTCCTGCAT CTCCACAGTT 
CAAGAGGGCA CTACACAGGC CTGGAGGAGG CTATCACCAG AACTGGAGGC TGGGGGCATC 
CAGACTGTGG AGGAGGAGCA GGTGGCTGAC CTGACCCTGA TGGGGGAGGG GGACATCAGC 
AGCCCCAACA GCACGCTGCT ACCACCACCA CCCCTTCCCC AAGACACACC AGGACCTACA 
AGACCAGGTA CCTCCAGGTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062212.1
CDS	100..4263
Translation	MYRAGEPGKRQSGPAPPRVRSVEVARGRAGYGFTLSGQAPCVLSCVMRGSPADFVGLRAGDQILAINEINVKKASHEDVVKLIGKCSGVLRMVISEGSSHVEPSSSDEEGGLCEGKGWLRPKLDSKALGINRAERVVEEVQSGGIFNMIFESPSLCASGSEPLKLKQRSLSESAALRLDVGQDSLCTPHPSMLSKEEISKVINDDSVFTVGLDNHDDFGLDASILNVAMVVGYLGSIELPSTSSNLEHDSLQAIRGCMRRLRAEQKIHSLVTMKVMHDCVQLVTDRAGVVAEYPAEKLAFSAVCPDDRRFFGLVTMQTNDDGCLAQEDEGALRTSCHVFMVDPDLFHHKIHQGIARRFGFACTADPDTSGCLEFPASSLPVLQFISVLYRDMGELIEGVRARAFLDGDADAHQNNSTSSNSDSGIGNFNQEEKSNRVLVVDLGGGSSRHGQGSSPGWESVSGRGSQPWSAPWNGTFCHDSEAGSPLETSPNTDRFWDLTKHSGPVFHMEVPPATLRSSIPPSKRGATGSSCGFNQRWLPVHVLQEWQCGHASDQESYTDSTDGWSSVNCGTLPPPMSKIPADRYRVEGSFAQAPLSTQKRDWSRKAFGMQNLFGPHRNVRKTKEDKKSSKLGRGVALAQTSQRTSARRSFGRSRRFSLTRSLDDLESATVSDGELTGADLKDCISNNSLSSNASLPSVQSCRRLRERRVASWAVSFERLLQDPVGVRYFSDFLRKEFSEENILFWQACECFSHVPAHDKKELSYRAREIFSKFLCSKATTPVNIDSQAQLADDILNAPHPDMFKEQQLQIFNLMKFDSYTRFLKSQLYQECVLAEVEGRTLPDSQQVPSSPASKHSISSDHSNVSTPKKLSGKSKSGRSLNEDVGEEDSEKKRKGAFFSWSRSRSTGRSQKKKDHGDHAHDALHANGGLCRRESQGSVSSAGSLDLSEACRTSALERDKAAKHCCVHLPDGTSCVVAVKSGFSIKEILSGLCERHGINGAAVDLFLVGGDKPLVLHQDSSILATRDLRLGKRTLFRLDLVPINRSVGLKAKPTKPVTEVLRPVVAKYGLDLGSLLVRLSGEKEPLDLGAPISSLDGQRVILEERDPSRGKVSTEKQKGAPVKQSSAVNSSPRNHSAMGEERTLGKSNSIKIRGENGKSARDPRLSKREESIAKIGKKKYQKINLDEAEEFFELISKAQSNRADDQRGLLRKEDLVLPEFLRLPPGSSELALSSPPPVKGFSKRAVTSHGQEGAVQTEESYSDSPATSPASAQSPCSAYSPGSAHSPGSAHSPGSAHSTPGPPGTAQPGEKPTKPSCISTVQEGTTQAWRRLSPELEAGGIQTVEEEQVADLTLMGEGDISSPNSTLLPPPPLPQDTPGPTRPGTSRF

Target ORF information:

RefSeq Version	NM_019339.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus regulator of G-protein signaling 12 (Rgs12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019339.1

ORF Insert Sequence:

ATGTACAGGG CTGGGGAGCC AGGGAAGCGG CAGTCTGGCC CTGCGCCCCC TCGGGTGCGG 
AGTGTGGAAG TGGCCCGAGG CAGAGCTGGC TACGGCTTCA CCCTGTCAGG ACAGGCACCC 
TGTGTGCTCA GCTGCGTCAT GAGGGGCAGC CCTGCAGATT TCGTGGGCCT CCGGGCTGGG 
GACCAGATAC TTGCCATCAA CGAAATCAAT GTGAAGAAAG CCTCACATGA AGATGTGGTG 
AAACTCATCG GGAAATGCTC TGGAGTGCTG CGCATGGTGA TCTCCGAGGG CAGCAGCCAT 
GTGGAGCCCT CTTCCAGCGA TGAAGAAGGC GGCCTGTGTG AGGGGAAAGG CTGGCTGAGG 
CCGAAACTTG ACTCTAAGGC TTTGGGTATA AACAGGGCAG AGAGGGTTGT GGAAGAGGTG 
CAGTCTGGTG GGATTTTCAA CATGATCTTT GAAAGCCCAA GTCTTTGTGC CAGTGGCTCT 
GAGCCCCTGA AACTGAAGCA GAGGTCCTTG TCAGAGTCAG CTGCTTTGCG GCTTGATGTT 
GGCCAGGACA GTCTCTGTAC ACCACATCCC AGCATGCTTT CCAAGGAAGA GATATCAAAG 
GTTATCAATG ACGATTCTGT GTTCACTGTA GGGCTGGACA ATCACGATGA CTTTGGACTA 
GATGCAAGTA TTTTAAATGT TGCCATGGTT GTGGGCTATT TGGGCTCCAT CGAGCTCCCT 
TCCACAAGTT CCAACCTGGA GCATGACAGT TTACAGGCTA TTCGTGGCTG CATGCGCCGC 
CTGCGGGCTG AGCAGAAGAT CCACTCACTA GTGACCATGA AGGTCATGCA CGACTGTGTG 
CAGCTGGTCA CTGACAGGGC TGGTGTGGTG GCAGAGTACC CTGCCGAGAA GCTGGCGTTC 
AGTGCAGTGT GCCCAGACGA CAGGCGGTTC TTCGGGCTGG TGACCATGCA GACCAATGAT 
GATGGGTGCT TGGCACAGGA AGATGAAGGT GCCCTGAGGA CATCCTGCCA TGTCTTCATG 
GTGGACCCAG ACTTATTTCA TCACAAGATC CACCAAGGCA TCGCGAGAAG ATTTGGATTT 
GCATGCACGG CAGATCCAGA CACGAGTGGC TGTTTGGAAT TCCCAGCATC TTCCCTCCCT 
GTGCTTCAGT TCATCTCCGT TCTGTACCGA GACATGGGTG AACTGATTGA GGGTGTGCGG 
GCCCGTGCCT TTCTAGATGG GGATGCCGAT GCCCACCAGA ATAATAGCAC CAGCAGCAAC 
AGTGACAGCG GCATTGGCAA TTTCAACCAG GAAGAGAAGA GCAACAGGGT GCTTGTAGTT 
GACCTGGGTG GGGGCTCGAG CAGGCATGGC CAGGGCAGTA GTCCGGGGTG GGAGAGTGTA 
AGTGGAAGAG GCTCACAGCC TTGGAGTGCA CCCTGGAATG GAACTTTCTG CCACGACTCA 
GAGGCGGGCT CCCCACTGGA AACCAGCCCC AACACTGACA GGTTCTGGGA CTTAACAAAG 
CATTCAGGGC CTGTTTTTCA CATGGAGGTC CCTCCAGCCA CCTTGAGGAG TTCTATACCC 
CCTTCCAAGA GGGGTGCTAC AGGTTCCAGC TGTGGTTTCA ACCAGAGGTG GCTCCCTGTC 
CATGTGCTAC AAGAGTGGCA GTGTGGACAT GCCAGTGACC AGGAGTCTTA CACAGACTCT 
ACGGACGGGT GGTCCAGTGT GAACTGTGGC ACACTGCCCC CTCCCATGAG CAAGATTCCT 
GCAGACCGAT ACCGGGTGGA GGGCAGCTTT GCTCAGGCCC CACTGAGTAC CCAGAAGAGG 
GACTGGTCTC GGAAGGCTTT CGGAATGCAA AACCTATTTG GACCCCATCG AAATGTTAGA 
AAGACCAAAG AGGACAAAAA GAGTTCCAAA TTGGGGCGGG GAGTTGCCCT GGCCCAGACT 
TCTCAGCGCA CTTCAGCTCG CAGATCCTTT GGACGATCTA GGAGATTCAG CCTCACGCGC 
TCCCTTGATG ATCTCGAGTC TGCAACTGTG TCTGATGGTG AGTTGACGGG AGCTGACCTG 
AAGGACTGCA TCAGCAACAA CAGCCTGAGC AGCAATGCCA GCCTCCCCAG TGTGCAGAGC 
TGCCGGCGCC TAAGAGAGAG GAGAGTTGCA AGCTGGGCAG TGTCATTTGA GCGTCTGCTG 
CAGGATCCTG TCGGCGTCCG CTACTTCTCC GATTTTCTAC GGAAGGAATT CAGTGAAGAG 
AACATTTTAT TCTGGCAGGC CTGTGAATGT TTCAGTCATG TCCCTGCACA TGACAAAAAA 
GAGCTCTCTT ACAGGGCCCG GGAGATTTTC AGTAAATTCC TGTGCAGCAA AGCCACCACT 
CCTGTCAACA TTGACAGCCA GGCCCAGCTG GCAGACGATA TTCTCAATGC ACCCCACCCC 
GACATGTTCA AGGAACAGCA GCTCCAGATT TTCAACCTGA TGAAGTTTGA CAGCTATACT 
CGATTTCTGA AATCCCAGCT GTACCAAGAA TGCGTCCTGG CAGAGGTGGA GGGGCGTACC 
CTCCCGGACT CCCAGCAGGT CCCCAGCAGC CCAGCTTCCA AGCACAGCAT CAGCTCTGAC 
CACTCCAATG TATCCACACC GAAAAAGTTA AGTGGAAAAT CAAAATCAGG ACGATCCCTG 
AATGAAGATG TTGGGGAAGA GGATAGTGAG AAAAAGCGCA AAGGGGCCTT TTTCTCTTGG 
TCAAGGAGCA GGAGCACGGG GCGATCCCAG AAGAAGAAGG ACCATGGTGA TCATGCCCAT 
GATGCCCTTC ATGCCAATGG AGGACTCTGC CGTCGGGAGT CCCAAGGCTC TGTCTCTTCT 
GCGGGGAGCC TAGACCTGTC AGAGGCCTGT AGGACCTCAG CACTCGAGAG AGATAAGGCT 
GCCAAACACT GCTGTGTCCA CCTCCCAGAC GGGACATCCT GTGTGGTTGC TGTCAAGTCC 
GGGTTTTCCA TCAAAGAGAT CCTGTCTGGA TTGTGTGAAC GGCATGGCAT CAATGGGGCT 
GCTGTGGACC TCTTCCTGGT GGGCGGAGAC AAGCCTCTGG TGCTGCATCA GGACAGCAGC 
ATCCTGGCCA CCAGGGACCT ACGGTTGGGA AAGCGTACTT TGTTTCGGCT GGATCTCGTT 
CCAATTAACC GGTCAGTGGG ACTCAAGGCC AAGCCCACCA AGCCAGTCAC GGAAGTGTTG 
CGGCCCGTGG TGGCCAAGTA CGGCCTGGAT CTGGGTAGCC TGCTGGTGAG GCTGAGTGGG 
GAGAAGGAGC CCCTGGACCT TGGTGCCCCT ATATCAAGTC TGGATGGACA GCGGGTCATC 
CTAGAGGAGA GGGATCCTTC TAGAGGGAAA GTGTCCACAG AGAAACAGAA AGGTGCTCCT 
GTCAAACAAA GCTCAGCTGT GAACTCCAGC CCCAGAAACC ACTCGGCTAT GGGAGAGGAG 
AGAACACTAG GCAAGTCTAA CTCTATTAAA ATAAGAGGAG AAAATGGAAA AAGTGCTAGG 
GATCCCCGGC TTTCAAAGAG AGAAGAATCT ATTGCAAAGA TTGGGAAAAA AAAATATCAG 
AAAATTAATT TGGACGAAGC AGAGGAGTTT TTTGAGCTCA TTTCCAAAGC TCAGAGCAAC 
AGAGCCGATG ACCAGCGTGG GCTGCTAAGA AAGGAAGACC TGGTACTGCC TGAGTTTCTT 
CGCTTACCTC CTGGTTCCTC AGAACTTGCC CTGTCCAGCC CACCCCCAGT CAAGGGCTTT 
AGCAAGAGAG CGGTTACAAG CCATGGCCAG GAGGGAGCAG TTCAGACAGA GGAGAGCTAC 
AGCGACAGCC CAGCTACCAG CCCTGCCTCA GCCCAGAGCC CCTGCTCAGC CTACAGCCCT 
GGCTCCGCCC ACAGCCCCGG CTCTGCCCAC AGCCCTGGCT CCGCCCACAG CACCCCTGGG 
CCTCCTGGGA CCGCCCAACC TGGAGAGAAG CCTACAAAGC CCTCCTGCAT CTCCACAGTT 
CAAGAGGGCA CTACACAGGC CTGGAGGAGG CTATCACCAG AACTGGAGGC TGGGGGCATC 
CAGACTGTGG AGGAGGAGCA GGTGGCTGAC CTGACCCTGA TGGGGGAGGG GGACATCAGC 
AGCCCCAACA GCACGCTGCT ACCACCACCA CCCCTTCCCC AAGACACACC AGGACCTACA 
AGACCAGGTA CCTCCAGGTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rgs12 ( XM_006251352.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006251414.1 Rattus norvegicus regulator of G-protein signaling 12 (Rgs12), transcript variant X4, mRNA.

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CloneID	ORa36328
Clone ID Related Accession (Same CDS sequence)	XM_006251352.3 , XM_006251351.3 , XM_006251353.3 , XM_008770350.2
Accession Version	XM_006251352.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4374bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	regulator of G-protein signaling 12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006251352.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACAGGG CTGGGGAGCC AGGGAAGCGG CAGTCTGGCC CTGCGCCCCC TCGGGTGCGG 
AGTGTGGAAG TGGCCCGAGG CAGAGCTGGC TACGGCTTCA CCCTGTCAGG ACAGGCACCC 
TGTGTGCTCA GCTGCGTCAT GAGGGGCAGC CCTGCAGATT TCGTGGGCCT CCGGGCTGGG 
GACCAGATAC TTGCCATCAA CGAAATCAAT GTGAAGAAAG CCTCACATGA AGATGTGGTG 
AAACTCATCG GGAAATGCTC TGGAGTGCTG CGCATGGTGA TCTCCGAGGG CAGCAGCCAT 
GTGGAGCCCT CTTCCAGCGA TGAAGAAGGC GGCCTGTGTG AGGGGAAAGG CTGGCTGAGG 
CCGAAACTTG ACTCTAAGGC TTTGGGTATA AACAGGGCAG AGAGGGTTGT GGAAGAGGTG 
CAGTCTGGTG GGATTTTCAA CATGATCTTT GAAAGCCCAA GTCTTTGTGC CAGTGGCTCT 
GAGCCCCTGA AACTGAAGCA GAGGTCCTTG TCAGAGTCAG CTGCTTTGCG GCTTGATGTT 
GGCCAGGACA GTCTCTGTAC ACCACATCCC AGCATGCTTT CCAAGGAAGA GATATCAAAG 
GTTATCAATG ACGATTCTGT GTTCACTGTA GGGCTGGACA ATCACGATGA CTTTGGACTA 
GATGCAAGTA TTTTAAATGT TGCCATGGTT GTGGGCTATT TGGGCTCCAT CGAGCTCCCT 
TCCACAAGTT CCAACCTGGA GCATGACAGT TTACAGGCTA TTCGTGGCTG CATGCGCCGC 
CTGCGGGCTG AGCAGAAGAT CCACTCACTA GTGACCATGA AGGTCATGCA CGACTGTGTG 
CAGCTGGTCA CTGACAGGGC TGGTGTGGTG GCAGAGTACC CTGCCGAGAA GCTGGCGTTC 
AGTGCAGTGT GCCCAGACGA CAGGCGGTTC TTCGGGCTGG TGACCATGCA GACCAATGAT 
GATGGGTGCT TGGCACAGGA AGATGAAGGT GCCCTGAGGA CATCCTGCCA TGTCTTCATG 
GTGGACCCAG ACTTATTTCA TCACAAGATC CACCAAGGCA TCGCGAGAAG ATTTGGATTT 
GCATGCACGG CAGATCCAGA CACGAGTGGC TGTTTGGAAT TCCCAGCATC TTCCCTCCCT 
GTGCTTCAGT TCATCTCCGT TCTGTACCGA GACATGGGTG AACTGATTGA GGGTGTGCGG 
GCCCGTGCCT TTCTAGATGG GGATGCCGAT GCCCACCAGA ATAATAGCAC CAGCAGCAAC 
AGTGACAGCG GCATTGGCAA TTTCAACCAG GAAGAGAAGA GCAACAGGGT GCTTGTAGTT 
GACCTGGGTG GGGGCTCGAG CAGGCATGGC CAGGGCAGTA GTCCGGGGTG GGAGAGTGTA 
AGTGGAAGAG GCTCACAGCC TTGGAGTGCA CCCTGGAATG GAACTTTCTG CCACGACTCA 
GAGGCGGGCT CCCCACTGGA AACCAGCCCC AACACTGACA GGTTCTGGGA CTTAACAAAG 
CATTCAGGGC CTGTTTTTCA CATGGAGGTC CCTCCAGCCA CCTTGAGGAG TTCTATACCC 
CCTTCCAAGA GGGGTGCTAC AGGTTCCAGC TGTGGTTTCA ACCAGAGGTG GCTCCCTGTC 
CATGTGCTAC AAGAGTGGCA GTGTGGACAT GCCAGTGACC AGGAGTCTTA CACAGACTCT 
ACGGACGGGT GGTCCAGTGT GAACTGTGGC ACACTGCCCC CTCCCATGAG CAAGATTCCT 
GCAGACCGAT ACCGGGTGGA GGGCAGCTTT GCTCAGGCCC CACTGAGTAC CCAGAAGAGG 
GACTGGTCTC GGAAGGCTTT CGGAATGCAA AACCTATTTG GACCCCATCG AAATGTTAGA 
AAGACCAAAG AGGACAAAAA GAGTTCCAAA TTGGGGCGGG GAGTTGCCCT GGCCCAGACT 
TCTCAGCGCA CTTCAGCTCG CAGATCCTTT GGACGATCTA GGAGATTCAG CCTCACGCGC 
TCCCTTGATG ATCTCGAGTC TGCAACTGTG TCTGATGGTG AGTTGACGGG AGCTGACCTG 
AAGGACTGCA TCAGCAACAA CAGCCTGAGC AGCAATGCCA GCCTCCCCAG TGTGCAGAGC 
TGCCGGCGCC TAAGAGAGAG GAGAGTTGCA AGCTGGGCAG TGTCATTTGA GCGTCTGCTG 
CAGGATCCTG TCGGCGTCCG CTACTTCTCC GATTTTCTAC GGAAGGAATT CAGTGAAGAG 
AACATTTTAT TCTGGCAGGC CTGTGAATGT TTCAGTCATG TCCCTGCACA TGACAAAAAA 
GAGCTCTCTT ACAGGGCCCG GGAGATTTTC AGTAAATTCC TGTGCAGCAA AGCCACCACT 
CCTGTCAACA TTGACAGCCA GGCCCAGCTG GCAGACGATA TTCTCAATGC ACCCCACCCC 
GACATGTTCA AGGAACAGCA GCTCCAGATT TTCAACCTGA TGAAGTTTGA CAGCTATACT 
CGATTTCTGA AATCCCAGCT GTACCAAGAA TGCGTCCTGG CAGAGGTGGA GGGGCGTACC 
CTCCCGGACT CCCAGCAGGT CCCCAGCAGC CCAGCTTCCA AGCACAGCAT CAGCTCTGAC 
CACTCCAATG TATCCACACC GAAAAAGTTA AGTGGAAAAT CAAAATCAGG ACGATCCCTG 
AATGAAGATG TTGGGGAAGA GGATAGTGAG AAAAAGCGCA AAGGGGCCTT TTTCTCTTGG 
TCAAGGAGCA GGAGCACGGG GCGATCCCAG AAGAAGAAGG ACCATGGTGA TCATGCCCAT 
GATGCCCTTC ATGCCAATGG AGGACTCTGC CGTCGGGAGT CCCAAGGCTC TGTCTCTTCT 
GCGGGGAGCC TAGACCTGTC AGAGGCCTGT AGGACCTCAG CACTCGAGAG AGATAAGGCT 
GCCAAACACT GCTGTGTCCA CCTCCCAGAC GGGACATCCT GTGTGGTTGC TGTCAAGTCC 
GGGTTTTCCA TCAAAGAGAT CCTGTCTGGA TTGTGTGAAC GGCATGGCAT CAATGGGGCT 
GCTGTGGACC TCTTCCTGGT GGGCGGAGAC AAGCCTCTGG TGCTGCATCA GGACAGCAGC 
ATCCTGGCCA CCAGGGACCT ACGGTTGGAA AAGCGTACTT TGTTTCGGCT GGATCTCGTT 
CCAATTAACC GGTCAGTGGG ACTCAAGGCC AAGCCCACCA AGCCAGTCAC GGAAGTGTTG 
CGGCCCGTGG TGGCCAAGTA CGGCCTGGAT CTGGGTAGCC TGCTGGTGAG GCTGAGTGGG 
GAGAAGGAGC CCCTGGACCT TGGTGCCCCT ATATCAAGTC TGGATGGACA GCGGGTCATC 
CTAGAGGAGA GGGATCCTTC TAGAGGGAAA GTGTCCACAG AGAAACAGAA AGGTGCTCCT 
GTCAAACAAA GCTCAGCTGT GAACTCCAGC CCCAGAAACC ACTCGGCTAT GGGAGAGGAG 
AGAACACTAG GCAAGTCTAA CTCTATTAAA ATAAGAGGAG AAAATGGAAA AAGTGCTAGG 
GATCCCCGGC TTTCAAAGAG AGAAGAATCT ATTGCAAAGA TTGGGAAAAA AAAATATCAG 
AAAATTAATT TGGACGAAGC AGAGGAGTTT TTTGAGCTCA TTTCCAAAGC TCAGAGCAAC 
AGAGCCGATG ACCAGCGTGG GCTGCTAAGA AAGGAAGACC TGGTACTGCC TGAGTTTCTT 
CGCTTACCTC CTGGTTCCTC AGAACTTGCC CTGTCCAGCC CACCCCCAGT CAAGGGCTTT 
AGCAAGAGAG CGGTTACAAG CCATGGCCAG GAGGGAGCAG TTCAGACAGA GGAGAGCTAC 
AGCGACAGCC CAGCTACCAG CCCTGCCTCA GCCCAGAGCC CCTGCTCAGC CTACAGCCCT 
GGCTCCGCCC ACAGCCCCGG CTCTGCCCAC AGCCCTGGCT CCGCCCACAG CACCCCTGGG 
CCTCCTGGGA CCGCCCAACC TGGAGAGAAG CCTACAAAGC CCTCCTGCAT CTCCACAGTT 
CAAGAGGGCA CTACACAGGC CTGGAGGAGG CTATCACCAG AACTGGAGGC TGGGGGCATC 
CAGACTGTGG AGGAGGAGCA GGTGGCTGAC CTGACCCTGA TGGGGGAGGG GGACATCAGC 
AGCCCCAACA GCACGCTGCT ACCACCACCA CCCCTTCCCC AAGACACACC AGGACCTACA 
AGACCAGGGG GCAGGGCCCG AGGCAGCGAA GGCCTCCCAG TCAACAGGAT CATCGACGTG 
GACCTCGTTA CGGGTGTGCC TCCCGGGTGG GGAGGCAGCA TTCTGGGGGT GCAGCTGGGT 
CCCAGGAGGG TGAAAGCCAA TGGACCCTTC TTACCAAACG GTCCTGGCCA GAGCACCATC 
CCAGGTGAGC CCACCAAGCC CAAGGCCAGC ACACACCATG CCACCTTTGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006251414.1
CDS	238..4611
Translation	MYRAGEPGKRQSGPAPPRVRSVEVARGRAGYGFTLSGQAPCVLSCVMRGSPADFVGLRAGDQILAINEINVKKASHEDVVKLIGKCSGVLRMVISEGSSHVEPSSSDEEGGLCEGKGWLRPKLDSKALGINRAERVVEEVQSGGIFNMIFESPSLCASGSEPLKLKQRSLSESAALRLDVGQDSLCTPHPSMLSKEEISKVINDDSVFTVGLDNHDDFGLDASILNVAMVVGYLGSIELPSTSSNLEHDSLQAIRGCMRRLRAEQKIHSLVTMKVMHDCVQLVTDRAGVVAEYPAEKLAFSAVCPDDRRFFGLVTMQTNDDGCLAQEDEGALRTSCHVFMVDPDLFHHKIHQGIARRFGFACTADPDTSGCLEFPASSLPVLQFISVLYRDMGELIEGVRARAFLDGDADAHQNNSTSSNSDSGIGNFNQEEKSNRVLVVDLGGGSSRHGQGSSPGWESVSGRGSQPWSAPWNGTFCHDSEAGSPLETSPNTDRFWDLTKHSGPVFHMEVPPATLRSSIPPSKRGATGSSCGFNQRWLPVHVLQEWQCGHASDQESYTDSTDGWSSVNCGTLPPPMSKIPADRYRVEGSFAQAPLSTQKRDWSRKAFGMQNLFGPHRNVRKTKEDKKSSKLGRGVALAQTSQRTSARRSFGRSRRFSLTRSLDDLESATVSDGELTGADLKDCISNNSLSSNASLPSVQSCRRLRERRVASWAVSFERLLQDPVGVRYFSDFLRKEFSEENILFWQACECFSHVPAHDKKELSYRAREIFSKFLCSKATTPVNIDSQAQLADDILNAPHPDMFKEQQLQIFNLMKFDSYTRFLKSQLYQECVLAEVEGRTLPDSQQVPSSPASKHSISSDHSNVSTPKKLSGKSKSGRSLNEDVGEEDSEKKRKGAFFSWSRSRSTGRSQKKKDHGDHAHDALHANGGLCRRESQGSVSSAGSLDLSEACRTSALERDKAAKHCCVHLPDGTSCVVAVKSGFSIKEILSGLCERHGINGAAVDLFLVGGDKPLVLHQDSSILATRDLRLEKRTLFRLDLVPINRSVGLKAKPTKPVTEVLRPVVAKYGLDLGSLLVRLSGEKEPLDLGAPISSLDGQRVILEERDPSRGKVSTEKQKGAPVKQSSAVNSSPRNHSAMGEERTLGKSNSIKIRGENGKSARDPRLSKREESIAKIGKKKYQKINLDEAEEFFELISKAQSNRADDQRGLLRKEDLVLPEFLRLPPGSSELALSSPPPVKGFSKRAVTSHGQEGAVQTEESYSDSPATSPASAQSPCSAYSPGSAHSPGSAHSPGSAHSTPGPPGTAQPGEKPTKPSCISTVQEGTTQAWRRLSPELEAGGIQTVEEEQVADLTLMGEGDISSPNSTLLPPPPLPQDTPGPTRPGGRARGSEGLPVNRIIDVDLVTGVPPGWGGSILGVQLGPRRVKANGPFLPNGPGQSTIPGEPTKPKASTHHATFV

Target ORF information:

RefSeq Version	XM_006251352.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus regulator of G-protein signaling 12 (Rgs12), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006251352.3

ORF Insert Sequence:

ATGTACAGGG CTGGGGAGCC AGGGAAGCGG CAGTCTGGCC CTGCGCCCCC TCGGGTGCGG 
AGTGTGGAAG TGGCCCGAGG CAGAGCTGGC TACGGCTTCA CCCTGTCAGG ACAGGCACCC 
TGTGTGCTCA GCTGCGTCAT GAGGGGCAGC CCTGCAGATT TCGTGGGCCT CCGGGCTGGG 
GACCAGATAC TTGCCATCAA CGAAATCAAT GTGAAGAAAG CCTCACATGA AGATGTGGTG 
AAACTCATCG GGAAATGCTC TGGAGTGCTG CGCATGGTGA TCTCCGAGGG CAGCAGCCAT 
GTGGAGCCCT CTTCCAGCGA TGAAGAAGGC GGCCTGTGTG AGGGGAAAGG CTGGCTGAGG 
CCGAAACTTG ACTCTAAGGC TTTGGGTATA AACAGGGCAG AGAGGGTTGT GGAAGAGGTG 
CAGTCTGGTG GGATTTTCAA CATGATCTTT GAAAGCCCAA GTCTTTGTGC CAGTGGCTCT 
GAGCCCCTGA AACTGAAGCA GAGGTCCTTG TCAGAGTCAG CTGCTTTGCG GCTTGATGTT 
GGCCAGGACA GTCTCTGTAC ACCACATCCC AGCATGCTTT CCAAGGAAGA GATATCAAAG 
GTTATCAATG ACGATTCTGT GTTCACTGTA GGGCTGGACA ATCACGATGA CTTTGGACTA 
GATGCAAGTA TTTTAAATGT TGCCATGGTT GTGGGCTATT TGGGCTCCAT CGAGCTCCCT 
TCCACAAGTT CCAACCTGGA GCATGACAGT TTACAGGCTA TTCGTGGCTG CATGCGCCGC 
CTGCGGGCTG AGCAGAAGAT CCACTCACTA GTGACCATGA AGGTCATGCA CGACTGTGTG 
CAGCTGGTCA CTGACAGGGC TGGTGTGGTG GCAGAGTACC CTGCCGAGAA GCTGGCGTTC 
AGTGCAGTGT GCCCAGACGA CAGGCGGTTC TTCGGGCTGG TGACCATGCA GACCAATGAT 
GATGGGTGCT TGGCACAGGA AGATGAAGGT GCCCTGAGGA CATCCTGCCA TGTCTTCATG 
GTGGACCCAG ACTTATTTCA TCACAAGATC CACCAAGGCA TCGCGAGAAG ATTTGGATTT 
GCATGCACGG CAGATCCAGA CACGAGTGGC TGTTTGGAAT TCCCAGCATC TTCCCTCCCT 
GTGCTTCAGT TCATCTCCGT TCTGTACCGA GACATGGGTG AACTGATTGA GGGTGTGCGG 
GCCCGTGCCT TTCTAGATGG GGATGCCGAT GCCCACCAGA ATAATAGCAC CAGCAGCAAC 
AGTGACAGCG GCATTGGCAA TTTCAACCAG GAAGAGAAGA GCAACAGGGT GCTTGTAGTT 
GACCTGGGTG GGGGCTCGAG CAGGCATGGC CAGGGCAGTA GTCCGGGGTG GGAGAGTGTA 
AGTGGAAGAG GCTCACAGCC TTGGAGTGCA CCCTGGAATG GAACTTTCTG CCACGACTCA 
GAGGCGGGCT CCCCACTGGA AACCAGCCCC AACACTGACA GGTTCTGGGA CTTAACAAAG 
CATTCAGGGC CTGTTTTTCA CATGGAGGTC CCTCCAGCCA CCTTGAGGAG TTCTATACCC 
CCTTCCAAGA GGGGTGCTAC AGGTTCCAGC TGTGGTTTCA ACCAGAGGTG GCTCCCTGTC 
CATGTGCTAC AAGAGTGGCA GTGTGGACAT GCCAGTGACC AGGAGTCTTA CACAGACTCT 
ACGGACGGGT GGTCCAGTGT GAACTGTGGC ACACTGCCCC CTCCCATGAG CAAGATTCCT 
GCAGACCGAT ACCGGGTGGA GGGCAGCTTT GCTCAGGCCC CACTGAGTAC CCAGAAGAGG 
GACTGGTCTC GGAAGGCTTT CGGAATGCAA AACCTATTTG GACCCCATCG AAATGTTAGA 
AAGACCAAAG AGGACAAAAA GAGTTCCAAA TTGGGGCGGG GAGTTGCCCT GGCCCAGACT 
TCTCAGCGCA CTTCAGCTCG CAGATCCTTT GGACGATCTA GGAGATTCAG CCTCACGCGC 
TCCCTTGATG ATCTCGAGTC TGCAACTGTG TCTGATGGTG AGTTGACGGG AGCTGACCTG 
AAGGACTGCA TCAGCAACAA CAGCCTGAGC AGCAATGCCA GCCTCCCCAG TGTGCAGAGC 
TGCCGGCGCC TAAGAGAGAG GAGAGTTGCA AGCTGGGCAG TGTCATTTGA GCGTCTGCTG 
CAGGATCCTG TCGGCGTCCG CTACTTCTCC GATTTTCTAC GGAAGGAATT CAGTGAAGAG 
AACATTTTAT TCTGGCAGGC CTGTGAATGT TTCAGTCATG TCCCTGCACA TGACAAAAAA 
GAGCTCTCTT ACAGGGCCCG GGAGATTTTC AGTAAATTCC TGTGCAGCAA AGCCACCACT 
CCTGTCAACA TTGACAGCCA GGCCCAGCTG GCAGACGATA TTCTCAATGC ACCCCACCCC 
GACATGTTCA AGGAACAGCA GCTCCAGATT TTCAACCTGA TGAAGTTTGA CAGCTATACT 
CGATTTCTGA AATCCCAGCT GTACCAAGAA TGCGTCCTGG CAGAGGTGGA GGGGCGTACC 
CTCCCGGACT CCCAGCAGGT CCCCAGCAGC CCAGCTTCCA AGCACAGCAT CAGCTCTGAC 
CACTCCAATG TATCCACACC GAAAAAGTTA AGTGGAAAAT CAAAATCAGG ACGATCCCTG 
AATGAAGATG TTGGGGAAGA GGATAGTGAG AAAAAGCGCA AAGGGGCCTT TTTCTCTTGG 
TCAAGGAGCA GGAGCACGGG GCGATCCCAG AAGAAGAAGG ACCATGGTGA TCATGCCCAT 
GATGCCCTTC ATGCCAATGG AGGACTCTGC CGTCGGGAGT CCCAAGGCTC TGTCTCTTCT 
GCGGGGAGCC TAGACCTGTC AGAGGCCTGT AGGACCTCAG CACTCGAGAG AGATAAGGCT 
GCCAAACACT GCTGTGTCCA CCTCCCAGAC GGGACATCCT GTGTGGTTGC TGTCAAGTCC 
GGGTTTTCCA TCAAAGAGAT CCTGTCTGGA TTGTGTGAAC GGCATGGCAT CAATGGGGCT 
GCTGTGGACC TCTTCCTGGT GGGCGGAGAC AAGCCTCTGG TGCTGCATCA GGACAGCAGC 
ATCCTGGCCA CCAGGGACCT ACGGTTGGAA AAGCGTACTT TGTTTCGGCT GGATCTCGTT 
CCAATTAACC GGTCAGTGGG ACTCAAGGCC AAGCCCACCA AGCCAGTCAC GGAAGTGTTG 
CGGCCCGTGG TGGCCAAGTA CGGCCTGGAT CTGGGTAGCC TGCTGGTGAG GCTGAGTGGG 
GAGAAGGAGC CCCTGGACCT TGGTGCCCCT ATATCAAGTC TGGATGGACA GCGGGTCATC 
CTAGAGGAGA GGGATCCTTC TAGAGGGAAA GTGTCCACAG AGAAACAGAA AGGTGCTCCT 
GTCAAACAAA GCTCAGCTGT GAACTCCAGC CCCAGAAACC ACTCGGCTAT GGGAGAGGAG 
AGAACACTAG GCAAGTCTAA CTCTATTAAA ATAAGAGGAG AAAATGGAAA AAGTGCTAGG 
GATCCCCGGC TTTCAAAGAG AGAAGAATCT ATTGCAAAGA TTGGGAAAAA AAAATATCAG 
AAAATTAATT TGGACGAAGC AGAGGAGTTT TTTGAGCTCA TTTCCAAAGC TCAGAGCAAC 
AGAGCCGATG ACCAGCGTGG GCTGCTAAGA AAGGAAGACC TGGTACTGCC TGAGTTTCTT 
CGCTTACCTC CTGGTTCCTC AGAACTTGCC CTGTCCAGCC CACCCCCAGT CAAGGGCTTT 
AGCAAGAGAG CGGTTACAAG CCATGGCCAG GAGGGAGCAG TTCAGACAGA GGAGAGCTAC 
AGCGACAGCC CAGCTACCAG CCCTGCCTCA GCCCAGAGCC CCTGCTCAGC CTACAGCCCT 
GGCTCCGCCC ACAGCCCCGG CTCTGCCCAC AGCCCTGGCT CCGCCCACAG CACCCCTGGG 
CCTCCTGGGA CCGCCCAACC TGGAGAGAAG CCTACAAAGC CCTCCTGCAT CTCCACAGTT 
CAAGAGGGCA CTACACAGGC CTGGAGGAGG CTATCACCAG AACTGGAGGC TGGGGGCATC 
CAGACTGTGG AGGAGGAGCA GGTGGCTGAC CTGACCCTGA TGGGGGAGGG GGACATCAGC 
AGCCCCAACA GCACGCTGCT ACCACCACCA CCCCTTCCCC AAGACACACC AGGACCTACA 
AGACCAGGGG GCAGGGCCCG AGGCAGCGAA GGCCTCCCAG TCAACAGGAT CATCGACGTG 
GACCTCGTTA CGGGTGTGCC TCCCGGGTGG GGAGGCAGCA TTCTGGGGGT GCAGCTGGGT 
CCCAGGAGGG TGAAAGCCAA TGGACCCTTC TTACCAAACG GTCCTGGCCA GAGCACCATC 
CCAGGTGAGC CCACCAAGCC CAAGGCCAGC ACACACCATG CCACCTTTGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rgs12 ( XM_006251351.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006251413.1 Rattus norvegicus regulator of G-protein signaling 12 (Rgs12), transcript variant X1, mRNA.

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CloneID	ORa36328
Clone ID Related Accession (Same CDS sequence)	XM_006251352.3 , XM_006251351.3 , XM_006251353.3 , XM_008770350.2
Accession Version	XM_006251351.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4374bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	regulator of G-protein signaling 12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006251351.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACAGGG CTGGGGAGCC AGGGAAGCGG CAGTCTGGCC CTGCGCCCCC TCGGGTGCGG 
AGTGTGGAAG TGGCCCGAGG CAGAGCTGGC TACGGCTTCA CCCTGTCAGG ACAGGCACCC 
TGTGTGCTCA GCTGCGTCAT GAGGGGCAGC CCTGCAGATT TCGTGGGCCT CCGGGCTGGG 
GACCAGATAC TTGCCATCAA CGAAATCAAT GTGAAGAAAG CCTCACATGA AGATGTGGTG 
AAACTCATCG GGAAATGCTC TGGAGTGCTG CGCATGGTGA TCTCCGAGGG CAGCAGCCAT 
GTGGAGCCCT CTTCCAGCGA TGAAGAAGGC GGCCTGTGTG AGGGGAAAGG CTGGCTGAGG 
CCGAAACTTG ACTCTAAGGC TTTGGGTATA AACAGGGCAG AGAGGGTTGT GGAAGAGGTG 
CAGTCTGGTG GGATTTTCAA CATGATCTTT GAAAGCCCAA GTCTTTGTGC CAGTGGCTCT 
GAGCCCCTGA AACTGAAGCA GAGGTCCTTG TCAGAGTCAG CTGCTTTGCG GCTTGATGTT 
GGCCAGGACA GTCTCTGTAC ACCACATCCC AGCATGCTTT CCAAGGAAGA GATATCAAAG 
GTTATCAATG ACGATTCTGT GTTCACTGTA GGGCTGGACA ATCACGATGA CTTTGGACTA 
GATGCAAGTA TTTTAAATGT TGCCATGGTT GTGGGCTATT TGGGCTCCAT CGAGCTCCCT 
TCCACAAGTT CCAACCTGGA GCATGACAGT TTACAGGCTA TTCGTGGCTG CATGCGCCGC 
CTGCGGGCTG AGCAGAAGAT CCACTCACTA GTGACCATGA AGGTCATGCA CGACTGTGTG 
CAGCTGGTCA CTGACAGGGC TGGTGTGGTG GCAGAGTACC CTGCCGAGAA GCTGGCGTTC 
AGTGCAGTGT GCCCAGACGA CAGGCGGTTC TTCGGGCTGG TGACCATGCA GACCAATGAT 
GATGGGTGCT TGGCACAGGA AGATGAAGGT GCCCTGAGGA CATCCTGCCA TGTCTTCATG 
GTGGACCCAG ACTTATTTCA TCACAAGATC CACCAAGGCA TCGCGAGAAG ATTTGGATTT 
GCATGCACGG CAGATCCAGA CACGAGTGGC TGTTTGGAAT TCCCAGCATC TTCCCTCCCT 
GTGCTTCAGT TCATCTCCGT TCTGTACCGA GACATGGGTG AACTGATTGA GGGTGTGCGG 
GCCCGTGCCT TTCTAGATGG GGATGCCGAT GCCCACCAGA ATAATAGCAC CAGCAGCAAC 
AGTGACAGCG GCATTGGCAA TTTCAACCAG GAAGAGAAGA GCAACAGGGT GCTTGTAGTT 
GACCTGGGTG GGGGCTCGAG CAGGCATGGC CAGGGCAGTA GTCCGGGGTG GGAGAGTGTA 
AGTGGAAGAG GCTCACAGCC TTGGAGTGCA CCCTGGAATG GAACTTTCTG CCACGACTCA 
GAGGCGGGCT CCCCACTGGA AACCAGCCCC AACACTGACA GGTTCTGGGA CTTAACAAAG 
CATTCAGGGC CTGTTTTTCA CATGGAGGTC CCTCCAGCCA CCTTGAGGAG TTCTATACCC 
CCTTCCAAGA GGGGTGCTAC AGGTTCCAGC TGTGGTTTCA ACCAGAGGTG GCTCCCTGTC 
CATGTGCTAC AAGAGTGGCA GTGTGGACAT GCCAGTGACC AGGAGTCTTA CACAGACTCT 
ACGGACGGGT GGTCCAGTGT GAACTGTGGC ACACTGCCCC CTCCCATGAG CAAGATTCCT 
GCAGACCGAT ACCGGGTGGA GGGCAGCTTT GCTCAGGCCC CACTGAGTAC CCAGAAGAGG 
GACTGGTCTC GGAAGGCTTT CGGAATGCAA AACCTATTTG GACCCCATCG AAATGTTAGA 
AAGACCAAAG AGGACAAAAA GAGTTCCAAA TTGGGGCGGG GAGTTGCCCT GGCCCAGACT 
TCTCAGCGCA CTTCAGCTCG CAGATCCTTT GGACGATCTA GGAGATTCAG CCTCACGCGC 
TCCCTTGATG ATCTCGAGTC TGCAACTGTG TCTGATGGTG AGTTGACGGG AGCTGACCTG 
AAGGACTGCA TCAGCAACAA CAGCCTGAGC AGCAATGCCA GCCTCCCCAG TGTGCAGAGC 
TGCCGGCGCC TAAGAGAGAG GAGAGTTGCA AGCTGGGCAG TGTCATTTGA GCGTCTGCTG 
CAGGATCCTG TCGGCGTCCG CTACTTCTCC GATTTTCTAC GGAAGGAATT CAGTGAAGAG 
AACATTTTAT TCTGGCAGGC CTGTGAATGT TTCAGTCATG TCCCTGCACA TGACAAAAAA 
GAGCTCTCTT ACAGGGCCCG GGAGATTTTC AGTAAATTCC TGTGCAGCAA AGCCACCACT 
CCTGTCAACA TTGACAGCCA GGCCCAGCTG GCAGACGATA TTCTCAATGC ACCCCACCCC 
GACATGTTCA AGGAACAGCA GCTCCAGATT TTCAACCTGA TGAAGTTTGA CAGCTATACT 
CGATTTCTGA AATCCCAGCT GTACCAAGAA TGCGTCCTGG CAGAGGTGGA GGGGCGTACC 
CTCCCGGACT CCCAGCAGGT CCCCAGCAGC CCAGCTTCCA AGCACAGCAT CAGCTCTGAC 
CACTCCAATG TATCCACACC GAAAAAGTTA AGTGGAAAAT CAAAATCAGG ACGATCCCTG 
AATGAAGATG TTGGGGAAGA GGATAGTGAG AAAAAGCGCA AAGGGGCCTT TTTCTCTTGG 
TCAAGGAGCA GGAGCACGGG GCGATCCCAG AAGAAGAAGG ACCATGGTGA TCATGCCCAT 
GATGCCCTTC ATGCCAATGG AGGACTCTGC CGTCGGGAGT CCCAAGGCTC TGTCTCTTCT 
GCGGGGAGCC TAGACCTGTC AGAGGCCTGT AGGACCTCAG CACTCGAGAG AGATAAGGCT 
GCCAAACACT GCTGTGTCCA CCTCCCAGAC GGGACATCCT GTGTGGTTGC TGTCAAGTCC 
GGGTTTTCCA TCAAAGAGAT CCTGTCTGGA TTGTGTGAAC GGCATGGCAT CAATGGGGCT 
GCTGTGGACC TCTTCCTGGT GGGCGGAGAC AAGCCTCTGG TGCTGCATCA GGACAGCAGC 
ATCCTGGCCA CCAGGGACCT ACGGTTGGAA AAGCGTACTT TGTTTCGGCT GGATCTCGTT 
CCAATTAACC GGTCAGTGGG ACTCAAGGCC AAGCCCACCA AGCCAGTCAC GGAAGTGTTG 
CGGCCCGTGG TGGCCAAGTA CGGCCTGGAT CTGGGTAGCC TGCTGGTGAG GCTGAGTGGG 
GAGAAGGAGC CCCTGGACCT TGGTGCCCCT ATATCAAGTC TGGATGGACA GCGGGTCATC 
CTAGAGGAGA GGGATCCTTC TAGAGGGAAA GTGTCCACAG AGAAACAGAA AGGTGCTCCT 
GTCAAACAAA GCTCAGCTGT GAACTCCAGC CCCAGAAACC ACTCGGCTAT GGGAGAGGAG 
AGAACACTAG GCAAGTCTAA CTCTATTAAA ATAAGAGGAG AAAATGGAAA AAGTGCTAGG 
GATCCCCGGC TTTCAAAGAG AGAAGAATCT ATTGCAAAGA TTGGGAAAAA AAAATATCAG 
AAAATTAATT TGGACGAAGC AGAGGAGTTT TTTGAGCTCA TTTCCAAAGC TCAGAGCAAC 
AGAGCCGATG ACCAGCGTGG GCTGCTAAGA AAGGAAGACC TGGTACTGCC TGAGTTTCTT 
CGCTTACCTC CTGGTTCCTC AGAACTTGCC CTGTCCAGCC CACCCCCAGT CAAGGGCTTT 
AGCAAGAGAG CGGTTACAAG CCATGGCCAG GAGGGAGCAG TTCAGACAGA GGAGAGCTAC 
AGCGACAGCC CAGCTACCAG CCCTGCCTCA GCCCAGAGCC CCTGCTCAGC CTACAGCCCT 
GGCTCCGCCC ACAGCCCCGG CTCTGCCCAC AGCCCTGGCT CCGCCCACAG CACCCCTGGG 
CCTCCTGGGA CCGCCCAACC TGGAGAGAAG CCTACAAAGC CCTCCTGCAT CTCCACAGTT 
CAAGAGGGCA CTACACAGGC CTGGAGGAGG CTATCACCAG AACTGGAGGC TGGGGGCATC 
CAGACTGTGG AGGAGGAGCA GGTGGCTGAC CTGACCCTGA TGGGGGAGGG GGACATCAGC 
AGCCCCAACA GCACGCTGCT ACCACCACCA CCCCTTCCCC AAGACACACC AGGACCTACA 
AGACCAGGGG GCAGGGCCCG AGGCAGCGAA GGCCTCCCAG TCAACAGGAT CATCGACGTG 
GACCTCGTTA CGGGTGTGCC TCCCGGGTGG GGAGGCAGCA TTCTGGGGGT GCAGCTGGGT 
CCCAGGAGGG TGAAAGCCAA TGGACCCTTC TTACCAAACG GTCCTGGCCA GAGCACCATC 
CCAGGTGAGC CCACCAAGCC CAAGGCCAGC ACACACCATG CCACCTTTGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006251413.1
CDS	234..4607
Translation	MYRAGEPGKRQSGPAPPRVRSVEVARGRAGYGFTLSGQAPCVLSCVMRGSPADFVGLRAGDQILAINEINVKKASHEDVVKLIGKCSGVLRMVISEGSSHVEPSSSDEEGGLCEGKGWLRPKLDSKALGINRAERVVEEVQSGGIFNMIFESPSLCASGSEPLKLKQRSLSESAALRLDVGQDSLCTPHPSMLSKEEISKVINDDSVFTVGLDNHDDFGLDASILNVAMVVGYLGSIELPSTSSNLEHDSLQAIRGCMRRLRAEQKIHSLVTMKVMHDCVQLVTDRAGVVAEYPAEKLAFSAVCPDDRRFFGLVTMQTNDDGCLAQEDEGALRTSCHVFMVDPDLFHHKIHQGIARRFGFACTADPDTSGCLEFPASSLPVLQFISVLYRDMGELIEGVRARAFLDGDADAHQNNSTSSNSDSGIGNFNQEEKSNRVLVVDLGGGSSRHGQGSSPGWESVSGRGSQPWSAPWNGTFCHDSEAGSPLETSPNTDRFWDLTKHSGPVFHMEVPPATLRSSIPPSKRGATGSSCGFNQRWLPVHVLQEWQCGHASDQESYTDSTDGWSSVNCGTLPPPMSKIPADRYRVEGSFAQAPLSTQKRDWSRKAFGMQNLFGPHRNVRKTKEDKKSSKLGRGVALAQTSQRTSARRSFGRSRRFSLTRSLDDLESATVSDGELTGADLKDCISNNSLSSNASLPSVQSCRRLRERRVASWAVSFERLLQDPVGVRYFSDFLRKEFSEENILFWQACECFSHVPAHDKKELSYRAREIFSKFLCSKATTPVNIDSQAQLADDILNAPHPDMFKEQQLQIFNLMKFDSYTRFLKSQLYQECVLAEVEGRTLPDSQQVPSSPASKHSISSDHSNVSTPKKLSGKSKSGRSLNEDVGEEDSEKKRKGAFFSWSRSRSTGRSQKKKDHGDHAHDALHANGGLCRRESQGSVSSAGSLDLSEACRTSALERDKAAKHCCVHLPDGTSCVVAVKSGFSIKEILSGLCERHGINGAAVDLFLVGGDKPLVLHQDSSILATRDLRLEKRTLFRLDLVPINRSVGLKAKPTKPVTEVLRPVVAKYGLDLGSLLVRLSGEKEPLDLGAPISSLDGQRVILEERDPSRGKVSTEKQKGAPVKQSSAVNSSPRNHSAMGEERTLGKSNSIKIRGENGKSARDPRLSKREESIAKIGKKKYQKINLDEAEEFFELISKAQSNRADDQRGLLRKEDLVLPEFLRLPPGSSELALSSPPPVKGFSKRAVTSHGQEGAVQTEESYSDSPATSPASAQSPCSAYSPGSAHSPGSAHSPGSAHSTPGPPGTAQPGEKPTKPSCISTVQEGTTQAWRRLSPELEAGGIQTVEEEQVADLTLMGEGDISSPNSTLLPPPPLPQDTPGPTRPGGRARGSEGLPVNRIIDVDLVTGVPPGWGGSILGVQLGPRRVKANGPFLPNGPGQSTIPGEPTKPKASTHHATFV

Target ORF information:

RefSeq Version	XM_006251351.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus regulator of G-protein signaling 12 (Rgs12), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006251351.3

ORF Insert Sequence:

ATGTACAGGG CTGGGGAGCC AGGGAAGCGG CAGTCTGGCC CTGCGCCCCC TCGGGTGCGG 
AGTGTGGAAG TGGCCCGAGG CAGAGCTGGC TACGGCTTCA CCCTGTCAGG ACAGGCACCC 
TGTGTGCTCA GCTGCGTCAT GAGGGGCAGC CCTGCAGATT TCGTGGGCCT CCGGGCTGGG 
GACCAGATAC TTGCCATCAA CGAAATCAAT GTGAAGAAAG CCTCACATGA AGATGTGGTG 
AAACTCATCG GGAAATGCTC TGGAGTGCTG CGCATGGTGA TCTCCGAGGG CAGCAGCCAT 
GTGGAGCCCT CTTCCAGCGA TGAAGAAGGC GGCCTGTGTG AGGGGAAAGG CTGGCTGAGG 
CCGAAACTTG ACTCTAAGGC TTTGGGTATA AACAGGGCAG AGAGGGTTGT GGAAGAGGTG 
CAGTCTGGTG GGATTTTCAA CATGATCTTT GAAAGCCCAA GTCTTTGTGC CAGTGGCTCT 
GAGCCCCTGA AACTGAAGCA GAGGTCCTTG TCAGAGTCAG CTGCTTTGCG GCTTGATGTT 
GGCCAGGACA GTCTCTGTAC ACCACATCCC AGCATGCTTT CCAAGGAAGA GATATCAAAG 
GTTATCAATG ACGATTCTGT GTTCACTGTA GGGCTGGACA ATCACGATGA CTTTGGACTA 
GATGCAAGTA TTTTAAATGT TGCCATGGTT GTGGGCTATT TGGGCTCCAT CGAGCTCCCT 
TCCACAAGTT CCAACCTGGA GCATGACAGT TTACAGGCTA TTCGTGGCTG CATGCGCCGC 
CTGCGGGCTG AGCAGAAGAT CCACTCACTA GTGACCATGA AGGTCATGCA CGACTGTGTG 
CAGCTGGTCA CTGACAGGGC TGGTGTGGTG GCAGAGTACC CTGCCGAGAA GCTGGCGTTC 
AGTGCAGTGT GCCCAGACGA CAGGCGGTTC TTCGGGCTGG TGACCATGCA GACCAATGAT 
GATGGGTGCT TGGCACAGGA AGATGAAGGT GCCCTGAGGA CATCCTGCCA TGTCTTCATG 
GTGGACCCAG ACTTATTTCA TCACAAGATC CACCAAGGCA TCGCGAGAAG ATTTGGATTT 
GCATGCACGG CAGATCCAGA CACGAGTGGC TGTTTGGAAT TCCCAGCATC TTCCCTCCCT 
GTGCTTCAGT TCATCTCCGT TCTGTACCGA GACATGGGTG AACTGATTGA GGGTGTGCGG 
GCCCGTGCCT TTCTAGATGG GGATGCCGAT GCCCACCAGA ATAATAGCAC CAGCAGCAAC 
AGTGACAGCG GCATTGGCAA TTTCAACCAG GAAGAGAAGA GCAACAGGGT GCTTGTAGTT 
GACCTGGGTG GGGGCTCGAG CAGGCATGGC CAGGGCAGTA GTCCGGGGTG GGAGAGTGTA 
AGTGGAAGAG GCTCACAGCC TTGGAGTGCA CCCTGGAATG GAACTTTCTG CCACGACTCA 
GAGGCGGGCT CCCCACTGGA AACCAGCCCC AACACTGACA GGTTCTGGGA CTTAACAAAG 
CATTCAGGGC CTGTTTTTCA CATGGAGGTC CCTCCAGCCA CCTTGAGGAG TTCTATACCC 
CCTTCCAAGA GGGGTGCTAC AGGTTCCAGC TGTGGTTTCA ACCAGAGGTG GCTCCCTGTC 
CATGTGCTAC AAGAGTGGCA GTGTGGACAT GCCAGTGACC AGGAGTCTTA CACAGACTCT 
ACGGACGGGT GGTCCAGTGT GAACTGTGGC ACACTGCCCC CTCCCATGAG CAAGATTCCT 
GCAGACCGAT ACCGGGTGGA GGGCAGCTTT GCTCAGGCCC CACTGAGTAC CCAGAAGAGG 
GACTGGTCTC GGAAGGCTTT CGGAATGCAA AACCTATTTG GACCCCATCG AAATGTTAGA 
AAGACCAAAG AGGACAAAAA GAGTTCCAAA TTGGGGCGGG GAGTTGCCCT GGCCCAGACT 
TCTCAGCGCA CTTCAGCTCG CAGATCCTTT GGACGATCTA GGAGATTCAG CCTCACGCGC 
TCCCTTGATG ATCTCGAGTC TGCAACTGTG TCTGATGGTG AGTTGACGGG AGCTGACCTG 
AAGGACTGCA TCAGCAACAA CAGCCTGAGC AGCAATGCCA GCCTCCCCAG TGTGCAGAGC 
TGCCGGCGCC TAAGAGAGAG GAGAGTTGCA AGCTGGGCAG TGTCATTTGA GCGTCTGCTG 
CAGGATCCTG TCGGCGTCCG CTACTTCTCC GATTTTCTAC GGAAGGAATT CAGTGAAGAG 
AACATTTTAT TCTGGCAGGC CTGTGAATGT TTCAGTCATG TCCCTGCACA TGACAAAAAA 
GAGCTCTCTT ACAGGGCCCG GGAGATTTTC AGTAAATTCC TGTGCAGCAA AGCCACCACT 
CCTGTCAACA TTGACAGCCA GGCCCAGCTG GCAGACGATA TTCTCAATGC ACCCCACCCC 
GACATGTTCA AGGAACAGCA GCTCCAGATT TTCAACCTGA TGAAGTTTGA CAGCTATACT 
CGATTTCTGA AATCCCAGCT GTACCAAGAA TGCGTCCTGG CAGAGGTGGA GGGGCGTACC 
CTCCCGGACT CCCAGCAGGT CCCCAGCAGC CCAGCTTCCA AGCACAGCAT CAGCTCTGAC 
CACTCCAATG TATCCACACC GAAAAAGTTA AGTGGAAAAT CAAAATCAGG ACGATCCCTG 
AATGAAGATG TTGGGGAAGA GGATAGTGAG AAAAAGCGCA AAGGGGCCTT TTTCTCTTGG 
TCAAGGAGCA GGAGCACGGG GCGATCCCAG AAGAAGAAGG ACCATGGTGA TCATGCCCAT 
GATGCCCTTC ATGCCAATGG AGGACTCTGC CGTCGGGAGT CCCAAGGCTC TGTCTCTTCT 
GCGGGGAGCC TAGACCTGTC AGAGGCCTGT AGGACCTCAG CACTCGAGAG AGATAAGGCT 
GCCAAACACT GCTGTGTCCA CCTCCCAGAC GGGACATCCT GTGTGGTTGC TGTCAAGTCC 
GGGTTTTCCA TCAAAGAGAT CCTGTCTGGA TTGTGTGAAC GGCATGGCAT CAATGGGGCT 
GCTGTGGACC TCTTCCTGGT GGGCGGAGAC AAGCCTCTGG TGCTGCATCA GGACAGCAGC 
ATCCTGGCCA CCAGGGACCT ACGGTTGGAA AAGCGTACTT TGTTTCGGCT GGATCTCGTT 
CCAATTAACC GGTCAGTGGG ACTCAAGGCC AAGCCCACCA AGCCAGTCAC GGAAGTGTTG 
CGGCCCGTGG TGGCCAAGTA CGGCCTGGAT CTGGGTAGCC TGCTGGTGAG GCTGAGTGGG 
GAGAAGGAGC CCCTGGACCT TGGTGCCCCT ATATCAAGTC TGGATGGACA GCGGGTCATC 
CTAGAGGAGA GGGATCCTTC TAGAGGGAAA GTGTCCACAG AGAAACAGAA AGGTGCTCCT 
GTCAAACAAA GCTCAGCTGT GAACTCCAGC CCCAGAAACC ACTCGGCTAT GGGAGAGGAG 
AGAACACTAG GCAAGTCTAA CTCTATTAAA ATAAGAGGAG AAAATGGAAA AAGTGCTAGG 
GATCCCCGGC TTTCAAAGAG AGAAGAATCT ATTGCAAAGA TTGGGAAAAA AAAATATCAG 
AAAATTAATT TGGACGAAGC AGAGGAGTTT TTTGAGCTCA TTTCCAAAGC TCAGAGCAAC 
AGAGCCGATG ACCAGCGTGG GCTGCTAAGA AAGGAAGACC TGGTACTGCC TGAGTTTCTT 
CGCTTACCTC CTGGTTCCTC AGAACTTGCC CTGTCCAGCC CACCCCCAGT CAAGGGCTTT 
AGCAAGAGAG CGGTTACAAG CCATGGCCAG GAGGGAGCAG TTCAGACAGA GGAGAGCTAC 
AGCGACAGCC CAGCTACCAG CCCTGCCTCA GCCCAGAGCC CCTGCTCAGC CTACAGCCCT 
GGCTCCGCCC ACAGCCCCGG CTCTGCCCAC AGCCCTGGCT CCGCCCACAG CACCCCTGGG 
CCTCCTGGGA CCGCCCAACC TGGAGAGAAG CCTACAAAGC CCTCCTGCAT CTCCACAGTT 
CAAGAGGGCA CTACACAGGC CTGGAGGAGG CTATCACCAG AACTGGAGGC TGGGGGCATC 
CAGACTGTGG AGGAGGAGCA GGTGGCTGAC CTGACCCTGA TGGGGGAGGG GGACATCAGC 
AGCCCCAACA GCACGCTGCT ACCACCACCA CCCCTTCCCC AAGACACACC AGGACCTACA 
AGACCAGGGG GCAGGGCCCG AGGCAGCGAA GGCCTCCCAG TCAACAGGAT CATCGACGTG 
GACCTCGTTA CGGGTGTGCC TCCCGGGTGG GGAGGCAGCA TTCTGGGGGT GCAGCTGGGT 
CCCAGGAGGG TGAAAGCCAA TGGACCCTTC TTACCAAACG GTCCTGGCCA GAGCACCATC 
CCAGGTGAGC CCACCAAGCC CAAGGCCAGC ACACACCATG CCACCTTTGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rgs12 ( XM_006251353.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006251415.1 Rattus norvegicus regulator of G-protein signaling 12 (Rgs12), transcript variant X3, mRNA.

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CloneID	ORa36328
Clone ID Related Accession (Same CDS sequence)	XM_006251352.3 , XM_006251351.3 , XM_006251353.3 , XM_008770350.2
Accession Version	XM_006251353.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4374bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	regulator of G-protein signaling 12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006251353.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACAGGG CTGGGGAGCC AGGGAAGCGG CAGTCTGGCC CTGCGCCCCC TCGGGTGCGG 
AGTGTGGAAG TGGCCCGAGG CAGAGCTGGC TACGGCTTCA CCCTGTCAGG ACAGGCACCC 
TGTGTGCTCA GCTGCGTCAT GAGGGGCAGC CCTGCAGATT TCGTGGGCCT CCGGGCTGGG 
GACCAGATAC TTGCCATCAA CGAAATCAAT GTGAAGAAAG CCTCACATGA AGATGTGGTG 
AAACTCATCG GGAAATGCTC TGGAGTGCTG CGCATGGTGA TCTCCGAGGG CAGCAGCCAT 
GTGGAGCCCT CTTCCAGCGA TGAAGAAGGC GGCCTGTGTG AGGGGAAAGG CTGGCTGAGG 
CCGAAACTTG ACTCTAAGGC TTTGGGTATA AACAGGGCAG AGAGGGTTGT GGAAGAGGTG 
CAGTCTGGTG GGATTTTCAA CATGATCTTT GAAAGCCCAA GTCTTTGTGC CAGTGGCTCT 
GAGCCCCTGA AACTGAAGCA GAGGTCCTTG TCAGAGTCAG CTGCTTTGCG GCTTGATGTT 
GGCCAGGACA GTCTCTGTAC ACCACATCCC AGCATGCTTT CCAAGGAAGA GATATCAAAG 
GTTATCAATG ACGATTCTGT GTTCACTGTA GGGCTGGACA ATCACGATGA CTTTGGACTA 
GATGCAAGTA TTTTAAATGT TGCCATGGTT GTGGGCTATT TGGGCTCCAT CGAGCTCCCT 
TCCACAAGTT CCAACCTGGA GCATGACAGT TTACAGGCTA TTCGTGGCTG CATGCGCCGC 
CTGCGGGCTG AGCAGAAGAT CCACTCACTA GTGACCATGA AGGTCATGCA CGACTGTGTG 
CAGCTGGTCA CTGACAGGGC TGGTGTGGTG GCAGAGTACC CTGCCGAGAA GCTGGCGTTC 
AGTGCAGTGT GCCCAGACGA CAGGCGGTTC TTCGGGCTGG TGACCATGCA GACCAATGAT 
GATGGGTGCT TGGCACAGGA AGATGAAGGT GCCCTGAGGA CATCCTGCCA TGTCTTCATG 
GTGGACCCAG ACTTATTTCA TCACAAGATC CACCAAGGCA TCGCGAGAAG ATTTGGATTT 
GCATGCACGG CAGATCCAGA CACGAGTGGC TGTTTGGAAT TCCCAGCATC TTCCCTCCCT 
GTGCTTCAGT TCATCTCCGT TCTGTACCGA GACATGGGTG AACTGATTGA GGGTGTGCGG 
GCCCGTGCCT TTCTAGATGG GGATGCCGAT GCCCACCAGA ATAATAGCAC CAGCAGCAAC 
AGTGACAGCG GCATTGGCAA TTTCAACCAG GAAGAGAAGA GCAACAGGGT GCTTGTAGTT 
GACCTGGGTG GGGGCTCGAG CAGGCATGGC CAGGGCAGTA GTCCGGGGTG GGAGAGTGTA 
AGTGGAAGAG GCTCACAGCC TTGGAGTGCA CCCTGGAATG GAACTTTCTG CCACGACTCA 
GAGGCGGGCT CCCCACTGGA AACCAGCCCC AACACTGACA GGTTCTGGGA CTTAACAAAG 
CATTCAGGGC CTGTTTTTCA CATGGAGGTC CCTCCAGCCA CCTTGAGGAG TTCTATACCC 
CCTTCCAAGA GGGGTGCTAC AGGTTCCAGC TGTGGTTTCA ACCAGAGGTG GCTCCCTGTC 
CATGTGCTAC AAGAGTGGCA GTGTGGACAT GCCAGTGACC AGGAGTCTTA CACAGACTCT 
ACGGACGGGT GGTCCAGTGT GAACTGTGGC ACACTGCCCC CTCCCATGAG CAAGATTCCT 
GCAGACCGAT ACCGGGTGGA GGGCAGCTTT GCTCAGGCCC CACTGAGTAC CCAGAAGAGG 
GACTGGTCTC GGAAGGCTTT CGGAATGCAA AACCTATTTG GACCCCATCG AAATGTTAGA 
AAGACCAAAG AGGACAAAAA GAGTTCCAAA TTGGGGCGGG GAGTTGCCCT GGCCCAGACT 
TCTCAGCGCA CTTCAGCTCG CAGATCCTTT GGACGATCTA GGAGATTCAG CCTCACGCGC 
TCCCTTGATG ATCTCGAGTC TGCAACTGTG TCTGATGGTG AGTTGACGGG AGCTGACCTG 
AAGGACTGCA TCAGCAACAA CAGCCTGAGC AGCAATGCCA GCCTCCCCAG TGTGCAGAGC 
TGCCGGCGCC TAAGAGAGAG GAGAGTTGCA AGCTGGGCAG TGTCATTTGA GCGTCTGCTG 
CAGGATCCTG TCGGCGTCCG CTACTTCTCC GATTTTCTAC GGAAGGAATT CAGTGAAGAG 
AACATTTTAT TCTGGCAGGC CTGTGAATGT TTCAGTCATG TCCCTGCACA TGACAAAAAA 
GAGCTCTCTT ACAGGGCCCG GGAGATTTTC AGTAAATTCC TGTGCAGCAA AGCCACCACT 
CCTGTCAACA TTGACAGCCA GGCCCAGCTG GCAGACGATA TTCTCAATGC ACCCCACCCC 
GACATGTTCA AGGAACAGCA GCTCCAGATT TTCAACCTGA TGAAGTTTGA CAGCTATACT 
CGATTTCTGA AATCCCAGCT GTACCAAGAA TGCGTCCTGG CAGAGGTGGA GGGGCGTACC 
CTCCCGGACT CCCAGCAGGT CCCCAGCAGC CCAGCTTCCA AGCACAGCAT CAGCTCTGAC 
CACTCCAATG TATCCACACC GAAAAAGTTA AGTGGAAAAT CAAAATCAGG ACGATCCCTG 
AATGAAGATG TTGGGGAAGA GGATAGTGAG AAAAAGCGCA AAGGGGCCTT TTTCTCTTGG 
TCAAGGAGCA GGAGCACGGG GCGATCCCAG AAGAAGAAGG ACCATGGTGA TCATGCCCAT 
GATGCCCTTC ATGCCAATGG AGGACTCTGC CGTCGGGAGT CCCAAGGCTC TGTCTCTTCT 
GCGGGGAGCC TAGACCTGTC AGAGGCCTGT AGGACCTCAG CACTCGAGAG AGATAAGGCT 
GCCAAACACT GCTGTGTCCA CCTCCCAGAC GGGACATCCT GTGTGGTTGC TGTCAAGTCC 
GGGTTTTCCA TCAAAGAGAT CCTGTCTGGA TTGTGTGAAC GGCATGGCAT CAATGGGGCT 
GCTGTGGACC TCTTCCTGGT GGGCGGAGAC AAGCCTCTGG TGCTGCATCA GGACAGCAGC 
ATCCTGGCCA CCAGGGACCT ACGGTTGGAA AAGCGTACTT TGTTTCGGCT GGATCTCGTT 
CCAATTAACC GGTCAGTGGG ACTCAAGGCC AAGCCCACCA AGCCAGTCAC GGAAGTGTTG 
CGGCCCGTGG TGGCCAAGTA CGGCCTGGAT CTGGGTAGCC TGCTGGTGAG GCTGAGTGGG 
GAGAAGGAGC CCCTGGACCT TGGTGCCCCT ATATCAAGTC TGGATGGACA GCGGGTCATC 
CTAGAGGAGA GGGATCCTTC TAGAGGGAAA GTGTCCACAG AGAAACAGAA AGGTGCTCCT 
GTCAAACAAA GCTCAGCTGT GAACTCCAGC CCCAGAAACC ACTCGGCTAT GGGAGAGGAG 
AGAACACTAG GCAAGTCTAA CTCTATTAAA ATAAGAGGAG AAAATGGAAA AAGTGCTAGG 
GATCCCCGGC TTTCAAAGAG AGAAGAATCT ATTGCAAAGA TTGGGAAAAA AAAATATCAG 
AAAATTAATT TGGACGAAGC AGAGGAGTTT TTTGAGCTCA TTTCCAAAGC TCAGAGCAAC 
AGAGCCGATG ACCAGCGTGG GCTGCTAAGA AAGGAAGACC TGGTACTGCC TGAGTTTCTT 
CGCTTACCTC CTGGTTCCTC AGAACTTGCC CTGTCCAGCC CACCCCCAGT CAAGGGCTTT 
AGCAAGAGAG CGGTTACAAG CCATGGCCAG GAGGGAGCAG TTCAGACAGA GGAGAGCTAC 
AGCGACAGCC CAGCTACCAG CCCTGCCTCA GCCCAGAGCC CCTGCTCAGC CTACAGCCCT 
GGCTCCGCCC ACAGCCCCGG CTCTGCCCAC AGCCCTGGCT CCGCCCACAG CACCCCTGGG 
CCTCCTGGGA CCGCCCAACC TGGAGAGAAG CCTACAAAGC CCTCCTGCAT CTCCACAGTT 
CAAGAGGGCA CTACACAGGC CTGGAGGAGG CTATCACCAG AACTGGAGGC TGGGGGCATC 
CAGACTGTGG AGGAGGAGCA GGTGGCTGAC CTGACCCTGA TGGGGGAGGG GGACATCAGC 
AGCCCCAACA GCACGCTGCT ACCACCACCA CCCCTTCCCC AAGACACACC AGGACCTACA 
AGACCAGGGG GCAGGGCCCG AGGCAGCGAA GGCCTCCCAG TCAACAGGAT CATCGACGTG 
GACCTCGTTA CGGGTGTGCC TCCCGGGTGG GGAGGCAGCA TTCTGGGGGT GCAGCTGGGT 
CCCAGGAGGG TGAAAGCCAA TGGACCCTTC TTACCAAACG GTCCTGGCCA GAGCACCATC 
CCAGGTGAGC CCACCAAGCC CAAGGCCAGC ACACACCATG CCACCTTTGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006251415.1
CDS	755..5128
Translation	MYRAGEPGKRQSGPAPPRVRSVEVARGRAGYGFTLSGQAPCVLSCVMRGSPADFVGLRAGDQILAINEINVKKASHEDVVKLIGKCSGVLRMVISEGSSHVEPSSSDEEGGLCEGKGWLRPKLDSKALGINRAERVVEEVQSGGIFNMIFESPSLCASGSEPLKLKQRSLSESAALRLDVGQDSLCTPHPSMLSKEEISKVINDDSVFTVGLDNHDDFGLDASILNVAMVVGYLGSIELPSTSSNLEHDSLQAIRGCMRRLRAEQKIHSLVTMKVMHDCVQLVTDRAGVVAEYPAEKLAFSAVCPDDRRFFGLVTMQTNDDGCLAQEDEGALRTSCHVFMVDPDLFHHKIHQGIARRFGFACTADPDTSGCLEFPASSLPVLQFISVLYRDMGELIEGVRARAFLDGDADAHQNNSTSSNSDSGIGNFNQEEKSNRVLVVDLGGGSSRHGQGSSPGWESVSGRGSQPWSAPWNGTFCHDSEAGSPLETSPNTDRFWDLTKHSGPVFHMEVPPATLRSSIPPSKRGATGSSCGFNQRWLPVHVLQEWQCGHASDQESYTDSTDGWSSVNCGTLPPPMSKIPADRYRVEGSFAQAPLSTQKRDWSRKAFGMQNLFGPHRNVRKTKEDKKSSKLGRGVALAQTSQRTSARRSFGRSRRFSLTRSLDDLESATVSDGELTGADLKDCISNNSLSSNASLPSVQSCRRLRERRVASWAVSFERLLQDPVGVRYFSDFLRKEFSEENILFWQACECFSHVPAHDKKELSYRAREIFSKFLCSKATTPVNIDSQAQLADDILNAPHPDMFKEQQLQIFNLMKFDSYTRFLKSQLYQECVLAEVEGRTLPDSQQVPSSPASKHSISSDHSNVSTPKKLSGKSKSGRSLNEDVGEEDSEKKRKGAFFSWSRSRSTGRSQKKKDHGDHAHDALHANGGLCRRESQGSVSSAGSLDLSEACRTSALERDKAAKHCCVHLPDGTSCVVAVKSGFSIKEILSGLCERHGINGAAVDLFLVGGDKPLVLHQDSSILATRDLRLEKRTLFRLDLVPINRSVGLKAKPTKPVTEVLRPVVAKYGLDLGSLLVRLSGEKEPLDLGAPISSLDGQRVILEERDPSRGKVSTEKQKGAPVKQSSAVNSSPRNHSAMGEERTLGKSNSIKIRGENGKSARDPRLSKREESIAKIGKKKYQKINLDEAEEFFELISKAQSNRADDQRGLLRKEDLVLPEFLRLPPGSSELALSSPPPVKGFSKRAVTSHGQEGAVQTEESYSDSPATSPASAQSPCSAYSPGSAHSPGSAHSPGSAHSTPGPPGTAQPGEKPTKPSCISTVQEGTTQAWRRLSPELEAGGIQTVEEEQVADLTLMGEGDISSPNSTLLPPPPLPQDTPGPTRPGGRARGSEGLPVNRIIDVDLVTGVPPGWGGSILGVQLGPRRVKANGPFLPNGPGQSTIPGEPTKPKASTHHATFV

Target ORF information:

RefSeq Version	XM_006251353.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus regulator of G-protein signaling 12 (Rgs12), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006251353.3

ORF Insert Sequence:

ATGTACAGGG CTGGGGAGCC AGGGAAGCGG CAGTCTGGCC CTGCGCCCCC TCGGGTGCGG 
AGTGTGGAAG TGGCCCGAGG CAGAGCTGGC TACGGCTTCA CCCTGTCAGG ACAGGCACCC 
TGTGTGCTCA GCTGCGTCAT GAGGGGCAGC CCTGCAGATT TCGTGGGCCT CCGGGCTGGG 
GACCAGATAC TTGCCATCAA CGAAATCAAT GTGAAGAAAG CCTCACATGA AGATGTGGTG 
AAACTCATCG GGAAATGCTC TGGAGTGCTG CGCATGGTGA TCTCCGAGGG CAGCAGCCAT 
GTGGAGCCCT CTTCCAGCGA TGAAGAAGGC GGCCTGTGTG AGGGGAAAGG CTGGCTGAGG 
CCGAAACTTG ACTCTAAGGC TTTGGGTATA AACAGGGCAG AGAGGGTTGT GGAAGAGGTG 
CAGTCTGGTG GGATTTTCAA CATGATCTTT GAAAGCCCAA GTCTTTGTGC CAGTGGCTCT 
GAGCCCCTGA AACTGAAGCA GAGGTCCTTG TCAGAGTCAG CTGCTTTGCG GCTTGATGTT 
GGCCAGGACA GTCTCTGTAC ACCACATCCC AGCATGCTTT CCAAGGAAGA GATATCAAAG 
GTTATCAATG ACGATTCTGT GTTCACTGTA GGGCTGGACA ATCACGATGA CTTTGGACTA 
GATGCAAGTA TTTTAAATGT TGCCATGGTT GTGGGCTATT TGGGCTCCAT CGAGCTCCCT 
TCCACAAGTT CCAACCTGGA GCATGACAGT TTACAGGCTA TTCGTGGCTG CATGCGCCGC 
CTGCGGGCTG AGCAGAAGAT CCACTCACTA GTGACCATGA AGGTCATGCA CGACTGTGTG 
CAGCTGGTCA CTGACAGGGC TGGTGTGGTG GCAGAGTACC CTGCCGAGAA GCTGGCGTTC 
AGTGCAGTGT GCCCAGACGA CAGGCGGTTC TTCGGGCTGG TGACCATGCA GACCAATGAT 
GATGGGTGCT TGGCACAGGA AGATGAAGGT GCCCTGAGGA CATCCTGCCA TGTCTTCATG 
GTGGACCCAG ACTTATTTCA TCACAAGATC CACCAAGGCA TCGCGAGAAG ATTTGGATTT 
GCATGCACGG CAGATCCAGA CACGAGTGGC TGTTTGGAAT TCCCAGCATC TTCCCTCCCT 
GTGCTTCAGT TCATCTCCGT TCTGTACCGA GACATGGGTG AACTGATTGA GGGTGTGCGG 
GCCCGTGCCT TTCTAGATGG GGATGCCGAT GCCCACCAGA ATAATAGCAC CAGCAGCAAC 
AGTGACAGCG GCATTGGCAA TTTCAACCAG GAAGAGAAGA GCAACAGGGT GCTTGTAGTT 
GACCTGGGTG GGGGCTCGAG CAGGCATGGC CAGGGCAGTA GTCCGGGGTG GGAGAGTGTA 
AGTGGAAGAG GCTCACAGCC TTGGAGTGCA CCCTGGAATG GAACTTTCTG CCACGACTCA 
GAGGCGGGCT CCCCACTGGA AACCAGCCCC AACACTGACA GGTTCTGGGA CTTAACAAAG 
CATTCAGGGC CTGTTTTTCA CATGGAGGTC CCTCCAGCCA CCTTGAGGAG TTCTATACCC 
CCTTCCAAGA GGGGTGCTAC AGGTTCCAGC TGTGGTTTCA ACCAGAGGTG GCTCCCTGTC 
CATGTGCTAC AAGAGTGGCA GTGTGGACAT GCCAGTGACC AGGAGTCTTA CACAGACTCT 
ACGGACGGGT GGTCCAGTGT GAACTGTGGC ACACTGCCCC CTCCCATGAG CAAGATTCCT 
GCAGACCGAT ACCGGGTGGA GGGCAGCTTT GCTCAGGCCC CACTGAGTAC CCAGAAGAGG 
GACTGGTCTC GGAAGGCTTT CGGAATGCAA AACCTATTTG GACCCCATCG AAATGTTAGA 
AAGACCAAAG AGGACAAAAA GAGTTCCAAA TTGGGGCGGG GAGTTGCCCT GGCCCAGACT 
TCTCAGCGCA CTTCAGCTCG CAGATCCTTT GGACGATCTA GGAGATTCAG CCTCACGCGC 
TCCCTTGATG ATCTCGAGTC TGCAACTGTG TCTGATGGTG AGTTGACGGG AGCTGACCTG 
AAGGACTGCA TCAGCAACAA CAGCCTGAGC AGCAATGCCA GCCTCCCCAG TGTGCAGAGC 
TGCCGGCGCC TAAGAGAGAG GAGAGTTGCA AGCTGGGCAG TGTCATTTGA GCGTCTGCTG 
CAGGATCCTG TCGGCGTCCG CTACTTCTCC GATTTTCTAC GGAAGGAATT CAGTGAAGAG 
AACATTTTAT TCTGGCAGGC CTGTGAATGT TTCAGTCATG TCCCTGCACA TGACAAAAAA 
GAGCTCTCTT ACAGGGCCCG GGAGATTTTC AGTAAATTCC TGTGCAGCAA AGCCACCACT 
CCTGTCAACA TTGACAGCCA GGCCCAGCTG GCAGACGATA TTCTCAATGC ACCCCACCCC 
GACATGTTCA AGGAACAGCA GCTCCAGATT TTCAACCTGA TGAAGTTTGA CAGCTATACT 
CGATTTCTGA AATCCCAGCT GTACCAAGAA TGCGTCCTGG CAGAGGTGGA GGGGCGTACC 
CTCCCGGACT CCCAGCAGGT CCCCAGCAGC CCAGCTTCCA AGCACAGCAT CAGCTCTGAC 
CACTCCAATG TATCCACACC GAAAAAGTTA AGTGGAAAAT CAAAATCAGG ACGATCCCTG 
AATGAAGATG TTGGGGAAGA GGATAGTGAG AAAAAGCGCA AAGGGGCCTT TTTCTCTTGG 
TCAAGGAGCA GGAGCACGGG GCGATCCCAG AAGAAGAAGG ACCATGGTGA TCATGCCCAT 
GATGCCCTTC ATGCCAATGG AGGACTCTGC CGTCGGGAGT CCCAAGGCTC TGTCTCTTCT 
GCGGGGAGCC TAGACCTGTC AGAGGCCTGT AGGACCTCAG CACTCGAGAG AGATAAGGCT 
GCCAAACACT GCTGTGTCCA CCTCCCAGAC GGGACATCCT GTGTGGTTGC TGTCAAGTCC 
GGGTTTTCCA TCAAAGAGAT CCTGTCTGGA TTGTGTGAAC GGCATGGCAT CAATGGGGCT 
GCTGTGGACC TCTTCCTGGT GGGCGGAGAC AAGCCTCTGG TGCTGCATCA GGACAGCAGC 
ATCCTGGCCA CCAGGGACCT ACGGTTGGAA AAGCGTACTT TGTTTCGGCT GGATCTCGTT 
CCAATTAACC GGTCAGTGGG ACTCAAGGCC AAGCCCACCA AGCCAGTCAC GGAAGTGTTG 
CGGCCCGTGG TGGCCAAGTA CGGCCTGGAT CTGGGTAGCC TGCTGGTGAG GCTGAGTGGG 
GAGAAGGAGC CCCTGGACCT TGGTGCCCCT ATATCAAGTC TGGATGGACA GCGGGTCATC 
CTAGAGGAGA GGGATCCTTC TAGAGGGAAA GTGTCCACAG AGAAACAGAA AGGTGCTCCT 
GTCAAACAAA GCTCAGCTGT GAACTCCAGC CCCAGAAACC ACTCGGCTAT GGGAGAGGAG 
AGAACACTAG GCAAGTCTAA CTCTATTAAA ATAAGAGGAG AAAATGGAAA AAGTGCTAGG 
GATCCCCGGC TTTCAAAGAG AGAAGAATCT ATTGCAAAGA TTGGGAAAAA AAAATATCAG 
AAAATTAATT TGGACGAAGC AGAGGAGTTT TTTGAGCTCA TTTCCAAAGC TCAGAGCAAC 
AGAGCCGATG ACCAGCGTGG GCTGCTAAGA AAGGAAGACC TGGTACTGCC TGAGTTTCTT 
CGCTTACCTC CTGGTTCCTC AGAACTTGCC CTGTCCAGCC CACCCCCAGT CAAGGGCTTT 
AGCAAGAGAG CGGTTACAAG CCATGGCCAG GAGGGAGCAG TTCAGACAGA GGAGAGCTAC 
AGCGACAGCC CAGCTACCAG CCCTGCCTCA GCCCAGAGCC CCTGCTCAGC CTACAGCCCT 
GGCTCCGCCC ACAGCCCCGG CTCTGCCCAC AGCCCTGGCT CCGCCCACAG CACCCCTGGG 
CCTCCTGGGA CCGCCCAACC TGGAGAGAAG CCTACAAAGC CCTCCTGCAT CTCCACAGTT 
CAAGAGGGCA CTACACAGGC CTGGAGGAGG CTATCACCAG AACTGGAGGC TGGGGGCATC 
CAGACTGTGG AGGAGGAGCA GGTGGCTGAC CTGACCCTGA TGGGGGAGGG GGACATCAGC 
AGCCCCAACA GCACGCTGCT ACCACCACCA CCCCTTCCCC AAGACACACC AGGACCTACA 
AGACCAGGGG GCAGGGCCCG AGGCAGCGAA GGCCTCCCAG TCAACAGGAT CATCGACGTG 
GACCTCGTTA CGGGTGTGCC TCCCGGGTGG GGAGGCAGCA TTCTGGGGGT GCAGCTGGGT 
CCCAGGAGGG TGAAAGCCAA TGGACCCTTC TTACCAAACG GTCCTGGCCA GAGCACCATC 
CCAGGTGAGC CCACCAAGCC CAAGGCCAGC ACACACCATG CCACCTTTGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rgs12 ( XM_008770350.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008768572.1 Rattus norvegicus regulator of G-protein signaling 12 (Rgs12), transcript variant X2, mRNA.

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CloneID	ORa36328
Clone ID Related Accession (Same CDS sequence)	XM_006251352.3 , XM_006251351.3 , XM_006251353.3 , XM_008770350.2
Accession Version	XM_008770350.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4374bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	regulator of G-protein signaling 12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008770350.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACAGGG CTGGGGAGCC AGGGAAGCGG CAGTCTGGCC CTGCGCCCCC TCGGGTGCGG 
AGTGTGGAAG TGGCCCGAGG CAGAGCTGGC TACGGCTTCA CCCTGTCAGG ACAGGCACCC 
TGTGTGCTCA GCTGCGTCAT GAGGGGCAGC CCTGCAGATT TCGTGGGCCT CCGGGCTGGG 
GACCAGATAC TTGCCATCAA CGAAATCAAT GTGAAGAAAG CCTCACATGA AGATGTGGTG 
AAACTCATCG GGAAATGCTC TGGAGTGCTG CGCATGGTGA TCTCCGAGGG CAGCAGCCAT 
GTGGAGCCCT CTTCCAGCGA TGAAGAAGGC GGCCTGTGTG AGGGGAAAGG CTGGCTGAGG 
CCGAAACTTG ACTCTAAGGC TTTGGGTATA AACAGGGCAG AGAGGGTTGT GGAAGAGGTG 
CAGTCTGGTG GGATTTTCAA CATGATCTTT GAAAGCCCAA GTCTTTGTGC CAGTGGCTCT 
GAGCCCCTGA AACTGAAGCA GAGGTCCTTG TCAGAGTCAG CTGCTTTGCG GCTTGATGTT 
GGCCAGGACA GTCTCTGTAC ACCACATCCC AGCATGCTTT CCAAGGAAGA GATATCAAAG 
GTTATCAATG ACGATTCTGT GTTCACTGTA GGGCTGGACA ATCACGATGA CTTTGGACTA 
GATGCAAGTA TTTTAAATGT TGCCATGGTT GTGGGCTATT TGGGCTCCAT CGAGCTCCCT 
TCCACAAGTT CCAACCTGGA GCATGACAGT TTACAGGCTA TTCGTGGCTG CATGCGCCGC 
CTGCGGGCTG AGCAGAAGAT CCACTCACTA GTGACCATGA AGGTCATGCA CGACTGTGTG 
CAGCTGGTCA CTGACAGGGC TGGTGTGGTG GCAGAGTACC CTGCCGAGAA GCTGGCGTTC 
AGTGCAGTGT GCCCAGACGA CAGGCGGTTC TTCGGGCTGG TGACCATGCA GACCAATGAT 
GATGGGTGCT TGGCACAGGA AGATGAAGGT GCCCTGAGGA CATCCTGCCA TGTCTTCATG 
GTGGACCCAG ACTTATTTCA TCACAAGATC CACCAAGGCA TCGCGAGAAG ATTTGGATTT 
GCATGCACGG CAGATCCAGA CACGAGTGGC TGTTTGGAAT TCCCAGCATC TTCCCTCCCT 
GTGCTTCAGT TCATCTCCGT TCTGTACCGA GACATGGGTG AACTGATTGA GGGTGTGCGG 
GCCCGTGCCT TTCTAGATGG GGATGCCGAT GCCCACCAGA ATAATAGCAC CAGCAGCAAC 
AGTGACAGCG GCATTGGCAA TTTCAACCAG GAAGAGAAGA GCAACAGGGT GCTTGTAGTT 
GACCTGGGTG GGGGCTCGAG CAGGCATGGC CAGGGCAGTA GTCCGGGGTG GGAGAGTGTA 
AGTGGAAGAG GCTCACAGCC TTGGAGTGCA CCCTGGAATG GAACTTTCTG CCACGACTCA 
GAGGCGGGCT CCCCACTGGA AACCAGCCCC AACACTGACA GGTTCTGGGA CTTAACAAAG 
CATTCAGGGC CTGTTTTTCA CATGGAGGTC CCTCCAGCCA CCTTGAGGAG TTCTATACCC 
CCTTCCAAGA GGGGTGCTAC AGGTTCCAGC TGTGGTTTCA ACCAGAGGTG GCTCCCTGTC 
CATGTGCTAC AAGAGTGGCA GTGTGGACAT GCCAGTGACC AGGAGTCTTA CACAGACTCT 
ACGGACGGGT GGTCCAGTGT GAACTGTGGC ACACTGCCCC CTCCCATGAG CAAGATTCCT 
GCAGACCGAT ACCGGGTGGA GGGCAGCTTT GCTCAGGCCC CACTGAGTAC CCAGAAGAGG 
GACTGGTCTC GGAAGGCTTT CGGAATGCAA AACCTATTTG GACCCCATCG AAATGTTAGA 
AAGACCAAAG AGGACAAAAA GAGTTCCAAA TTGGGGCGGG GAGTTGCCCT GGCCCAGACT 
TCTCAGCGCA CTTCAGCTCG CAGATCCTTT GGACGATCTA GGAGATTCAG CCTCACGCGC 
TCCCTTGATG ATCTCGAGTC TGCAACTGTG TCTGATGGTG AGTTGACGGG AGCTGACCTG 
AAGGACTGCA TCAGCAACAA CAGCCTGAGC AGCAATGCCA GCCTCCCCAG TGTGCAGAGC 
TGCCGGCGCC TAAGAGAGAG GAGAGTTGCA AGCTGGGCAG TGTCATTTGA GCGTCTGCTG 
CAGGATCCTG TCGGCGTCCG CTACTTCTCC GATTTTCTAC GGAAGGAATT CAGTGAAGAG 
AACATTTTAT TCTGGCAGGC CTGTGAATGT TTCAGTCATG TCCCTGCACA TGACAAAAAA 
GAGCTCTCTT ACAGGGCCCG GGAGATTTTC AGTAAATTCC TGTGCAGCAA AGCCACCACT 
CCTGTCAACA TTGACAGCCA GGCCCAGCTG GCAGACGATA TTCTCAATGC ACCCCACCCC 
GACATGTTCA AGGAACAGCA GCTCCAGATT TTCAACCTGA TGAAGTTTGA CAGCTATACT 
CGATTTCTGA AATCCCAGCT GTACCAAGAA TGCGTCCTGG CAGAGGTGGA GGGGCGTACC 
CTCCCGGACT CCCAGCAGGT CCCCAGCAGC CCAGCTTCCA AGCACAGCAT CAGCTCTGAC 
CACTCCAATG TATCCACACC GAAAAAGTTA AGTGGAAAAT CAAAATCAGG ACGATCCCTG 
AATGAAGATG TTGGGGAAGA GGATAGTGAG AAAAAGCGCA AAGGGGCCTT TTTCTCTTGG 
TCAAGGAGCA GGAGCACGGG GCGATCCCAG AAGAAGAAGG ACCATGGTGA TCATGCCCAT 
GATGCCCTTC ATGCCAATGG AGGACTCTGC CGTCGGGAGT CCCAAGGCTC TGTCTCTTCT 
GCGGGGAGCC TAGACCTGTC AGAGGCCTGT AGGACCTCAG CACTCGAGAG AGATAAGGCT 
GCCAAACACT GCTGTGTCCA CCTCCCAGAC GGGACATCCT GTGTGGTTGC TGTCAAGTCC 
GGGTTTTCCA TCAAAGAGAT CCTGTCTGGA TTGTGTGAAC GGCATGGCAT CAATGGGGCT 
GCTGTGGACC TCTTCCTGGT GGGCGGAGAC AAGCCTCTGG TGCTGCATCA GGACAGCAGC 
ATCCTGGCCA CCAGGGACCT ACGGTTGGAA AAGCGTACTT TGTTTCGGCT GGATCTCGTT 
CCAATTAACC GGTCAGTGGG ACTCAAGGCC AAGCCCACCA AGCCAGTCAC GGAAGTGTTG 
CGGCCCGTGG TGGCCAAGTA CGGCCTGGAT CTGGGTAGCC TGCTGGTGAG GCTGAGTGGG 
GAGAAGGAGC CCCTGGACCT TGGTGCCCCT ATATCAAGTC TGGATGGACA GCGGGTCATC 
CTAGAGGAGA GGGATCCTTC TAGAGGGAAA GTGTCCACAG AGAAACAGAA AGGTGCTCCT 
GTCAAACAAA GCTCAGCTGT GAACTCCAGC CCCAGAAACC ACTCGGCTAT GGGAGAGGAG 
AGAACACTAG GCAAGTCTAA CTCTATTAAA ATAAGAGGAG AAAATGGAAA AAGTGCTAGG 
GATCCCCGGC TTTCAAAGAG AGAAGAATCT ATTGCAAAGA TTGGGAAAAA AAAATATCAG 
AAAATTAATT TGGACGAAGC AGAGGAGTTT TTTGAGCTCA TTTCCAAAGC TCAGAGCAAC 
AGAGCCGATG ACCAGCGTGG GCTGCTAAGA AAGGAAGACC TGGTACTGCC TGAGTTTCTT 
CGCTTACCTC CTGGTTCCTC AGAACTTGCC CTGTCCAGCC CACCCCCAGT CAAGGGCTTT 
AGCAAGAGAG CGGTTACAAG CCATGGCCAG GAGGGAGCAG TTCAGACAGA GGAGAGCTAC 
AGCGACAGCC CAGCTACCAG CCCTGCCTCA GCCCAGAGCC CCTGCTCAGC CTACAGCCCT 
GGCTCCGCCC ACAGCCCCGG CTCTGCCCAC AGCCCTGGCT CCGCCCACAG CACCCCTGGG 
CCTCCTGGGA CCGCCCAACC TGGAGAGAAG CCTACAAAGC CCTCCTGCAT CTCCACAGTT 
CAAGAGGGCA CTACACAGGC CTGGAGGAGG CTATCACCAG AACTGGAGGC TGGGGGCATC 
CAGACTGTGG AGGAGGAGCA GGTGGCTGAC CTGACCCTGA TGGGGGAGGG GGACATCAGC 
AGCCCCAACA GCACGCTGCT ACCACCACCA CCCCTTCCCC AAGACACACC AGGACCTACA 
AGACCAGGGG GCAGGGCCCG AGGCAGCGAA GGCCTCCCAG TCAACAGGAT CATCGACGTG 
GACCTCGTTA CGGGTGTGCC TCCCGGGTGG GGAGGCAGCA TTCTGGGGGT GCAGCTGGGT 
CCCAGGAGGG TGAAAGCCAA TGGACCCTTC TTACCAAACG GTCCTGGCCA GAGCACCATC 
CCAGGTGAGC CCACCAAGCC CAAGGCCAGC ACACACCATG CCACCTTTGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008768572.1
CDS	768..5141
Translation	MYRAGEPGKRQSGPAPPRVRSVEVARGRAGYGFTLSGQAPCVLSCVMRGSPADFVGLRAGDQILAINEINVKKASHEDVVKLIGKCSGVLRMVISEGSSHVEPSSSDEEGGLCEGKGWLRPKLDSKALGINRAERVVEEVQSGGIFNMIFESPSLCASGSEPLKLKQRSLSESAALRLDVGQDSLCTPHPSMLSKEEISKVINDDSVFTVGLDNHDDFGLDASILNVAMVVGYLGSIELPSTSSNLEHDSLQAIRGCMRRLRAEQKIHSLVTMKVMHDCVQLVTDRAGVVAEYPAEKLAFSAVCPDDRRFFGLVTMQTNDDGCLAQEDEGALRTSCHVFMVDPDLFHHKIHQGIARRFGFACTADPDTSGCLEFPASSLPVLQFISVLYRDMGELIEGVRARAFLDGDADAHQNNSTSSNSDSGIGNFNQEEKSNRVLVVDLGGGSSRHGQGSSPGWESVSGRGSQPWSAPWNGTFCHDSEAGSPLETSPNTDRFWDLTKHSGPVFHMEVPPATLRSSIPPSKRGATGSSCGFNQRWLPVHVLQEWQCGHASDQESYTDSTDGWSSVNCGTLPPPMSKIPADRYRVEGSFAQAPLSTQKRDWSRKAFGMQNLFGPHRNVRKTKEDKKSSKLGRGVALAQTSQRTSARRSFGRSRRFSLTRSLDDLESATVSDGELTGADLKDCISNNSLSSNASLPSVQSCRRLRERRVASWAVSFERLLQDPVGVRYFSDFLRKEFSEENILFWQACECFSHVPAHDKKELSYRAREIFSKFLCSKATTPVNIDSQAQLADDILNAPHPDMFKEQQLQIFNLMKFDSYTRFLKSQLYQECVLAEVEGRTLPDSQQVPSSPASKHSISSDHSNVSTPKKLSGKSKSGRSLNEDVGEEDSEKKRKGAFFSWSRSRSTGRSQKKKDHGDHAHDALHANGGLCRRESQGSVSSAGSLDLSEACRTSALERDKAAKHCCVHLPDGTSCVVAVKSGFSIKEILSGLCERHGINGAAVDLFLVGGDKPLVLHQDSSILATRDLRLEKRTLFRLDLVPINRSVGLKAKPTKPVTEVLRPVVAKYGLDLGSLLVRLSGEKEPLDLGAPISSLDGQRVILEERDPSRGKVSTEKQKGAPVKQSSAVNSSPRNHSAMGEERTLGKSNSIKIRGENGKSARDPRLSKREESIAKIGKKKYQKINLDEAEEFFELISKAQSNRADDQRGLLRKEDLVLPEFLRLPPGSSELALSSPPPVKGFSKRAVTSHGQEGAVQTEESYSDSPATSPASAQSPCSAYSPGSAHSPGSAHSPGSAHSTPGPPGTAQPGEKPTKPSCISTVQEGTTQAWRRLSPELEAGGIQTVEEEQVADLTLMGEGDISSPNSTLLPPPPLPQDTPGPTRPGGRARGSEGLPVNRIIDVDLVTGVPPGWGGSILGVQLGPRRVKANGPFLPNGPGQSTIPGEPTKPKASTHHATFV

Target ORF information:

RefSeq Version	XM_008770350.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus regulator of G-protein signaling 12 (Rgs12), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008770350.2

ORF Insert Sequence:

ATGTACAGGG CTGGGGAGCC AGGGAAGCGG CAGTCTGGCC CTGCGCCCCC TCGGGTGCGG 
AGTGTGGAAG TGGCCCGAGG CAGAGCTGGC TACGGCTTCA CCCTGTCAGG ACAGGCACCC 
TGTGTGCTCA GCTGCGTCAT GAGGGGCAGC CCTGCAGATT TCGTGGGCCT CCGGGCTGGG 
GACCAGATAC TTGCCATCAA CGAAATCAAT GTGAAGAAAG CCTCACATGA AGATGTGGTG 
AAACTCATCG GGAAATGCTC TGGAGTGCTG CGCATGGTGA TCTCCGAGGG CAGCAGCCAT 
GTGGAGCCCT CTTCCAGCGA TGAAGAAGGC GGCCTGTGTG AGGGGAAAGG CTGGCTGAGG 
CCGAAACTTG ACTCTAAGGC TTTGGGTATA AACAGGGCAG AGAGGGTTGT GGAAGAGGTG 
CAGTCTGGTG GGATTTTCAA CATGATCTTT GAAAGCCCAA GTCTTTGTGC CAGTGGCTCT 
GAGCCCCTGA AACTGAAGCA GAGGTCCTTG TCAGAGTCAG CTGCTTTGCG GCTTGATGTT 
GGCCAGGACA GTCTCTGTAC ACCACATCCC AGCATGCTTT CCAAGGAAGA GATATCAAAG 
GTTATCAATG ACGATTCTGT GTTCACTGTA GGGCTGGACA ATCACGATGA CTTTGGACTA 
GATGCAAGTA TTTTAAATGT TGCCATGGTT GTGGGCTATT TGGGCTCCAT CGAGCTCCCT 
TCCACAAGTT CCAACCTGGA GCATGACAGT TTACAGGCTA TTCGTGGCTG CATGCGCCGC 
CTGCGGGCTG AGCAGAAGAT CCACTCACTA GTGACCATGA AGGTCATGCA CGACTGTGTG 
CAGCTGGTCA CTGACAGGGC TGGTGTGGTG GCAGAGTACC CTGCCGAGAA GCTGGCGTTC 
AGTGCAGTGT GCCCAGACGA CAGGCGGTTC TTCGGGCTGG TGACCATGCA GACCAATGAT 
GATGGGTGCT TGGCACAGGA AGATGAAGGT GCCCTGAGGA CATCCTGCCA TGTCTTCATG 
GTGGACCCAG ACTTATTTCA TCACAAGATC CACCAAGGCA TCGCGAGAAG ATTTGGATTT 
GCATGCACGG CAGATCCAGA CACGAGTGGC TGTTTGGAAT TCCCAGCATC TTCCCTCCCT 
GTGCTTCAGT TCATCTCCGT TCTGTACCGA GACATGGGTG AACTGATTGA GGGTGTGCGG 
GCCCGTGCCT TTCTAGATGG GGATGCCGAT GCCCACCAGA ATAATAGCAC CAGCAGCAAC 
AGTGACAGCG GCATTGGCAA TTTCAACCAG GAAGAGAAGA GCAACAGGGT GCTTGTAGTT 
GACCTGGGTG GGGGCTCGAG CAGGCATGGC CAGGGCAGTA GTCCGGGGTG GGAGAGTGTA 
AGTGGAAGAG GCTCACAGCC TTGGAGTGCA CCCTGGAATG GAACTTTCTG CCACGACTCA 
GAGGCGGGCT CCCCACTGGA AACCAGCCCC AACACTGACA GGTTCTGGGA CTTAACAAAG 
CATTCAGGGC CTGTTTTTCA CATGGAGGTC CCTCCAGCCA CCTTGAGGAG TTCTATACCC 
CCTTCCAAGA GGGGTGCTAC AGGTTCCAGC TGTGGTTTCA ACCAGAGGTG GCTCCCTGTC 
CATGTGCTAC AAGAGTGGCA GTGTGGACAT GCCAGTGACC AGGAGTCTTA CACAGACTCT 
ACGGACGGGT GGTCCAGTGT GAACTGTGGC ACACTGCCCC CTCCCATGAG CAAGATTCCT 
GCAGACCGAT ACCGGGTGGA GGGCAGCTTT GCTCAGGCCC CACTGAGTAC CCAGAAGAGG 
GACTGGTCTC GGAAGGCTTT CGGAATGCAA AACCTATTTG GACCCCATCG AAATGTTAGA 
AAGACCAAAG AGGACAAAAA GAGTTCCAAA TTGGGGCGGG GAGTTGCCCT GGCCCAGACT 
TCTCAGCGCA CTTCAGCTCG CAGATCCTTT GGACGATCTA GGAGATTCAG CCTCACGCGC 
TCCCTTGATG ATCTCGAGTC TGCAACTGTG TCTGATGGTG AGTTGACGGG AGCTGACCTG 
AAGGACTGCA TCAGCAACAA CAGCCTGAGC AGCAATGCCA GCCTCCCCAG TGTGCAGAGC 
TGCCGGCGCC TAAGAGAGAG GAGAGTTGCA AGCTGGGCAG TGTCATTTGA GCGTCTGCTG 
CAGGATCCTG TCGGCGTCCG CTACTTCTCC GATTTTCTAC GGAAGGAATT CAGTGAAGAG 
AACATTTTAT TCTGGCAGGC CTGTGAATGT TTCAGTCATG TCCCTGCACA TGACAAAAAA 
GAGCTCTCTT ACAGGGCCCG GGAGATTTTC AGTAAATTCC TGTGCAGCAA AGCCACCACT 
CCTGTCAACA TTGACAGCCA GGCCCAGCTG GCAGACGATA TTCTCAATGC ACCCCACCCC 
GACATGTTCA AGGAACAGCA GCTCCAGATT TTCAACCTGA TGAAGTTTGA CAGCTATACT 
CGATTTCTGA AATCCCAGCT GTACCAAGAA TGCGTCCTGG CAGAGGTGGA GGGGCGTACC 
CTCCCGGACT CCCAGCAGGT CCCCAGCAGC CCAGCTTCCA AGCACAGCAT CAGCTCTGAC 
CACTCCAATG TATCCACACC GAAAAAGTTA AGTGGAAAAT CAAAATCAGG ACGATCCCTG 
AATGAAGATG TTGGGGAAGA GGATAGTGAG AAAAAGCGCA AAGGGGCCTT TTTCTCTTGG 
TCAAGGAGCA GGAGCACGGG GCGATCCCAG AAGAAGAAGG ACCATGGTGA TCATGCCCAT 
GATGCCCTTC ATGCCAATGG AGGACTCTGC CGTCGGGAGT CCCAAGGCTC TGTCTCTTCT 
GCGGGGAGCC TAGACCTGTC AGAGGCCTGT AGGACCTCAG CACTCGAGAG AGATAAGGCT 
GCCAAACACT GCTGTGTCCA CCTCCCAGAC GGGACATCCT GTGTGGTTGC TGTCAAGTCC 
GGGTTTTCCA TCAAAGAGAT CCTGTCTGGA TTGTGTGAAC GGCATGGCAT CAATGGGGCT 
GCTGTGGACC TCTTCCTGGT GGGCGGAGAC AAGCCTCTGG TGCTGCATCA GGACAGCAGC 
ATCCTGGCCA CCAGGGACCT ACGGTTGGAA AAGCGTACTT TGTTTCGGCT GGATCTCGTT 
CCAATTAACC GGTCAGTGGG ACTCAAGGCC AAGCCCACCA AGCCAGTCAC GGAAGTGTTG 
CGGCCCGTGG TGGCCAAGTA CGGCCTGGAT CTGGGTAGCC TGCTGGTGAG GCTGAGTGGG 
GAGAAGGAGC CCCTGGACCT TGGTGCCCCT ATATCAAGTC TGGATGGACA GCGGGTCATC 
CTAGAGGAGA GGGATCCTTC TAGAGGGAAA GTGTCCACAG AGAAACAGAA AGGTGCTCCT 
GTCAAACAAA GCTCAGCTGT GAACTCCAGC CCCAGAAACC ACTCGGCTAT GGGAGAGGAG 
AGAACACTAG GCAAGTCTAA CTCTATTAAA ATAAGAGGAG AAAATGGAAA AAGTGCTAGG 
GATCCCCGGC TTTCAAAGAG AGAAGAATCT ATTGCAAAGA TTGGGAAAAA AAAATATCAG 
AAAATTAATT TGGACGAAGC AGAGGAGTTT TTTGAGCTCA TTTCCAAAGC TCAGAGCAAC 
AGAGCCGATG ACCAGCGTGG GCTGCTAAGA AAGGAAGACC TGGTACTGCC TGAGTTTCTT 
CGCTTACCTC CTGGTTCCTC AGAACTTGCC CTGTCCAGCC CACCCCCAGT CAAGGGCTTT 
AGCAAGAGAG CGGTTACAAG CCATGGCCAG GAGGGAGCAG TTCAGACAGA GGAGAGCTAC 
AGCGACAGCC CAGCTACCAG CCCTGCCTCA GCCCAGAGCC CCTGCTCAGC CTACAGCCCT 
GGCTCCGCCC ACAGCCCCGG CTCTGCCCAC AGCCCTGGCT CCGCCCACAG CACCCCTGGG 
CCTCCTGGGA CCGCCCAACC TGGAGAGAAG CCTACAAAGC CCTCCTGCAT CTCCACAGTT 
CAAGAGGGCA CTACACAGGC CTGGAGGAGG CTATCACCAG AACTGGAGGC TGGGGGCATC 
CAGACTGTGG AGGAGGAGCA GGTGGCTGAC CTGACCCTGA TGGGGGAGGG GGACATCAGC 
AGCCCCAACA GCACGCTGCT ACCACCACCA CCCCTTCCCC AAGACACACC AGGACCTACA 
AGACCAGGGG GCAGGGCCCG AGGCAGCGAA GGCCTCCCAG TCAACAGGAT CATCGACGTG 
GACCTCGTTA CGGGTGTGCC TCCCGGGTGG GGAGGCAGCA TTCTGGGGGT GCAGCTGGGT 
CCCAGGAGGG TGAAAGCCAA TGGACCCTTC TTACCAAACG GTCCTGGCCA GAGCACCATC 
CCAGGTGAGC CCACCAAGCC CAAGGCCAGC ACACACCATG CCACCTTTGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Selective role for RGS12 as a Ras/Raf/MEK scaffold in nerve growth factor-mediated differentiation.
The EMBO journal26(8)2029-40(2007 Apr)
Willard MD,Willard FS,Li X,Cappell SD,Snider WD,Siderovski DP

Localization of the GoLoco motif carrier regulator of G-protein signalling 12 and 14 proteins in monkey and rat brain.
The European journal of neuroscience23(11)2971-82(2006 Jun)
López-Aranda MF,Acevedo MJ,Carballo FJ,Gutiérrez A,Khan ZU

Calcium-sensing receptor activation of rho involves filamin and rho-guanine nucleotide exchange factor.
Endocrinology143(10)3830-8(2002 Oct)
Pi M,Spurney RF,Tu Q,Hinson T,Quarles LD

RGS12 and RGS14 GoLoco motifs are G alpha(i) interaction sites with guanine nucleotide dissociation inhibitor Activity.
The Journal of biological chemistry276(31)29275-81(2001 Aug)
Kimple RJ,De Vries L,Tronchère H,Behe CI,Morris RA,Gist Farquhar M,Siderovski DP

Tyrosine-kinase-dependent recruitment of RGS12 to the N-type calcium channel.
Nature408(6813)723-7(2000 Dec)
Schiff ML,Siderovski DP,Jordan JD,Brothers G,Snow B,De Vries L,Ortiz DF,Diversé-Pierluissi M

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Rgs12 cDNA ORF clone, Rattus norvegicus(Norway rat)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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