Npepps cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Npepps
Entrez Gene ID	50558
Full Name	aminopeptidase puromycin sensitive
Synonyms	Psa
General protein information	Preferred Names aminopeptidase puromycin sensitive Names puromycin-sensitive aminopeptidase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q31
Summary	protein and enzyme activity are detected in the hippocampus at all developmental stages [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_080395.1	NP_536320.1	puromycin-sensitive aminopeptidase precursor
XM_017597478.1	XP_017452967.1	puromycin-sensitive aminopeptidase isoform X1

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Psa	NP_728614.1
Arabidopsis thaliana (thale cress)	APM1	NP_195035.2
Oryza sativa (rice)	Os02g0218200	NP_001046303.1
Schizosaccharomyces pombe (fission yeast)	ape2	NP_595998.1
Macaca mulatta (Rhesus monkey)	NPEPPS	XP_001082709.2
Rattus norvegicus (Norway rat)	Npepps	NP_536320.1
Gallus gallus (chicken)	NPEPPS	XP_001234986.3
Caenorhabditis elegans (roundworm)	pam-1	NP_001023210.1
Saccharomyces cerevisiae (baker's yeast)	APE2	NP_012765.3
Eremothecium gossypii	AGOS_AAL127W	NP_982415.2
Homo sapiens (human)	NPEPPS	NP_006301.3
Canis lupus familiaris (dog)	NPEPPS	XP_537659.4
Mus musculus (house mouse)	Npepps	NP_032968.2
Xenopus tropicalis (tropical clawed frog)	npepps	NP_001072690.1
Neurospora crassa	NCU09228	XP_959172.1
Pan troglodytes (chimpanzee)	NPEPPS	XP_001173517.2
Bos taurus (cattle)	NPEPPS	NP_001180088.1
Kluyveromyces lactis	KLLA0C11033g	XP_452692.2
Magnaporthe oryzae (rice blast fungus)	MGG_16472	XP_003710702.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005728	XP_556379.3
Danio rerio (zebrafish)	npepps	NP_001157499.1

Gene Ontology
Bibliography

Related articles in PubMed

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Hypoxia enhances transcriptional activity of androgen receptor through hypoxia-inducible factor-1α in a low androgen environment.
Mitani T, Yamaji R, Higashimura Y, Harada N, Nakano Y, Inui H
The Journal of steroid biochemistry and molecular biology123(1-2)58-64(2011 Jan)

MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis.
Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W
Immunology and cell biology88(8)851-6(2010 Nov-Dec)

Large-scale proteomics and phosphoproteomics of urinary exosomes.
Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal of the American Society of Nephrology : JASN20(2)363-79(2009 Feb)

Gene expression profile of the rat eye iridocorneal angle: NEIBank expressed sequence tag analysis.
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Investigative ophthalmology & visual science45(9)3081-90(2004 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Npepps gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Npepps cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa11734	NM_080395.1 Latest version!	Rattus norvegicus aminopeptidase puromycin sensitive (Npepps), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ORa57448	XM_017597478.1 Latest version!	Rattus norvegicus aminopeptidase puromycin sensitive (Npepps), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Npepps ( NM_080395.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_536320.1 Rattus norvegicus aminopeptidase puromycin sensitive (Npepps), mRNA.

Price & Availability↑

CloneID	ORa11734
Clone ID Related Accession (Same CDS sequence)	NM_080395.1
Accession Version	NM_080395.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2763bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1527350400000
Organism	Rattus norvegicus(Norway rat)
Product	puromycin-sensitive aminopeptidase precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DV216619.1, AB062596.1, CA512355.1, CA510536.1, H32677.1, DY316730.1, CA503636.1 and BC105844.1. On or before Oct 28, 2007 this sequence version replaced XM_001081366.1, XM_340889.3, XM_001074205.1, XM_001074182.1. Sequence Note: This RefSeq record was created from transcript sequence data from multiple strains because no single transcript from the same strain was available for the full length of the gene. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGGCTGG CAGCCGCCGT CCCCTCCCTC GCTCGCCGCC TGCTCCTCCT CGGCTCTCCG 
CCTCCTCCCC TCCTTCTCCT CCTCAGCCGC TCCTCTAGCC GCCGCCGCCG CCTCCACAGC 
CTGGGCCTCG CCGCGATGCC GGAGAAGCGG CCCTTCGAGC GGCTGCCCAC CGAGGTGTCG 
CCCATCAACT ACAGCCTCTG CCTCAAGCCC GATTTGCTGG ACTTCACCTT CGAGGGCAAG 
CTGGAGGCCG CCGCCCAGGT AAGGCAGGCA ACCAATCAGA TTGTGATGAA TTGTGCTGAT 
ATTGACATTA TTACAGCTTC ATATGCGCCA GAAGGAGATG AAGAAATCCA TGCGACAGGA 
TTTAACTATC AGAATGAAGA TGAGAAAGTC ACCTTGTCTT TTCCTAGTAC TCTCCAAACA 
GGTACAGGAA CCTTAAAGAT AGATTTTGTT GGAGAACTGA ACGACAAAAT GAAAGGTTTC 
TACAGAAGTA GATATACCAC CCCCGCCGGC GAGGTGCGCT ATGCTGCTGT AACACAGTTT 
GAGGCTACTG ATGCCCGAAG GGCTTTTCCT TGCTGGGATG AGCCTGCTAT CAAAGCAACT 
TTTGATATCT CGTTGGTTGT GCCTAAAGAT AGAGTGGCTT TATCAAATAT GAATGTCATT 
GACAGGAAAC CATACCCTGA TGATGAAAAC TTAGTGGAAG TGAAGTTTGC TCGCACACCT 
GTTATGTCTA CATATCTGGT GGCATTTGTT GTGGGTGAAT ATGACTTTGT AGAAACAAGG 
TCAAAAGATG GTGTGTGTGT CCGTGTTTAC ACCCCTGTTG GCAAAGCAGA GCAAGGAAAA 
TTTGCACTAG AGGTTGCTGC TAAAACCTTG CCTTTTTATA AAGACTACTT CAATGTTCCT 
TATCCTCTAC CTAAAATTGA TCTCATTGCT ATTGCTGACT TTGCAGCTGG TGCCATGGAG 
AACTGGGGCC TTGTTACTTA TAGGGAGACG GCATTGCTTA TTGATCCAAA AAACTCGTGT 
TCTTCATCAC GCCAGTGGGT TGCTCTGGTT GTGGGACATG AACTCGCCCA TCAATGGTTT 
GGAAATCTTG TTACTATGGA ATGGTGGACT CATCTCTGGT TGAACGAAGG CTTTGCATCC 
TGGATTGAGT ACCTTTGTGT AGACCACTGC TTCCCAGAGT ATGATATCTG GACTCAGTTT 
GTTTCTGCAG ATTATACCCG TGCCCAGGAA CTCGATGCCT TAGATAACAG CCATCCTATT 
GAAGTCAGTG TGGGCCATCC ATCTGAGGTT GATGAGATAT TTGATGCTAT ATCATACAGC 
AAAGGTGCAT CTGTAATCCG AATGCTACAT GACTACATTG GCGATAAGGA CTTTAAGAAA 
GGAATGAATA TGTATTTAAC CAAGTTCCAA CAAAAGAATG CTGCCACAGA GGATCTTTGG 
GAAAGTTTGG AAAGTGCCAG TGGCAAACCC ATAGCCGCTG TGATGAACAC CTGGACCAAA 
CAAATGGGGT TCCCTCTCAT TTATGTGGAA GCTGAACAGG TAGAAGATGA CAGAGTGCTG 
AAGCTGTCTC AAAAGAAGTT CTGTGCCAGC GGACCTTATG TCGGTGAAGA CTGTCCTCAG 
TGGATGGTTC CTATCACGAT TTCAACTAGT GAAGATCCCA ACCAGGCTAA ACTGAAAATA 
CTAATGGATA AGCCAGAGAT GAGTGTGGTT TTGAAAAATG TCAAACCAGA CCAATGGGTA 
AAGCTAAACC TGGGAACAGT TGGGTTTTAT CGAACCCAAT ACAGCTCTGC TATGCTGGAA 
AGTTTATTAC CAGGCATTCG TGACCTTTCT CTGCCCCCAG TGGATCGGCT TGGATTACAG 
AATGACCTCT TTTCTCTGGC TCGAGCTGGA ATCATTAGCA CTGTAGAGGT TCTAAAAGTC 
ATGGAGGCTT TTGTGAATGA GCCCAATTAT ACTGTGTGGA GCGACCTGAG CTGTAACCTG 
GGGATTCTTT CAACTCTCTT GTCCCACACA GACTTCTATG AGGAAATCCA GGAGTTTGTC 
AAAGATGTCT TCTCACCTAT AGGAGAGAGA CTGGGCTGGG ACCCCAAACC TGGAGAAGGT 
CATCTAGACG CACTCCTGAG GGGCTTGGTG CTGGGCAAAC TGGGAAAAGC AGGGCATAAG 
GCAACTTTGG AAGAAGCCCG CCGTCGGTTT AAGGAGCACG TGGAAGGGAA GCAGATTCTT 
TCTGCTGACC TAAGGAGTCC TGTTTATCTT ACTGTTTTAA AGCATGGTGA TGGCACTACC 
TTAGACATTA TGCTAAAGCT TCACAAACAA GCTGATATGC AAGAAGAGAA AAACAGAATC 
GAAAGAGTTC TTGGGGCTAC TCTTTCTCCT GAACTGATTC AAAAAGTCCT CACTTTTGCA 
CTTTCAGAAG AGGTACGTCC ACAGGACACT GTGTCAGTAA TTGGTGGAGT GGCTGGAGGC 
AGCAAGCATG GGAGGAAAGC TGCTTGGAAA TTCATAAAGG ACAACTGGGA AGAGCTTTAT 
AATCGATACC AGGGAGGGTT TTTAATATCC AGACTCATAA AGCTGTCAGT TGAGGGGTTT 
GCAGTTGATA AAATGGCTGG AGAAGTTAAG GCTTTCTTCG AGAGTCACCC AGCTCCTTCA 
GCCGAACGTA CCATCCAGCA GTGTTGTGAG AATATCCTGC TGAACGCTGC TTGGCTAAAG 
CGGGATGCTG ACAGCATTCA CCAATACCTC CTGCAGCGGA AAGCCTCCCC TCCCTCGGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_536320.1
CDS	103..2865
Translation	MWLAAAVPSLARRLLLLGSPPPPLLLLLSRSSSRRRRLHSLGLAAMPEKRPFERLPTEVSPINYSLCLKPDLLDFTFEGKLEAAAQVRQATNQIVMNCADIDIITASYAPEGDEEIHATGFNYQNEDEKVTLSFPSTLQTGTGTLKIDFVGELNDKMKGFYRSRYTTPAGEVRYAAVTQFEATDARRAFPCWDEPAIKATFDISLVVPKDRVALSNMNVIDRKPYPDDENLVEVKFARTPVMSTYLVAFVVGEYDFVETRSKDGVCVRVYTPVGKAEQGKFALEVAAKTLPFYKDYFNVPYPLPKIDLIAIADFAAGAMENWGLVTYRETALLIDPKNSCSSSRQWVALVVGHELAHQWFGNLVTMEWWTHLWLNEGFASWIEYLCVDHCFPEYDIWTQFVSADYTRAQELDALDNSHPIEVSVGHPSEVDEIFDAISYSKGASVIRMLHDYIGDKDFKKGMNMYLTKFQQKNAATEDLWESLESASGKPIAAVMNTWTKQMGFPLIYVEAEQVEDDRVLKLSQKKFCASGPYVGEDCPQWMVPITISTSEDPNQAKLKILMDKPEMSVVLKNVKPDQWVKLNLGTVGFYRTQYSSAMLESLLPGIRDLSLPPVDRLGLQNDLFSLARAGIISTVEVLKVMEAFVNEPNYTVWSDLSCNLGILSTLLSHTDFYEEIQEFVKDVFSPIGERLGWDPKPGEGHLDALLRGLVLGKLGKAGHKATLEEARRRFKEHVEGKQILSADLRSPVYLTVLKHGDGTTLDIMLKLHKQADMQEEKNRIERVLGATLSPELIQKVLTFALSEEVRPQDTVSVIGGVAGGSKHGRKAAWKFIKDNWEELYNRYQGGFLISRLIKLSVEGFAVDKMAGEVKAFFESHPAPSAERTIQQCCENILLNAAWLKRDADSIHQYLLQRKASPPSV

Target ORF information:

RefSeq Version	NM_080395.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus aminopeptidase puromycin sensitive (Npepps), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080395.1

ORF Insert Sequence:

ATGTGGCTGG CAGCCGCCGT CCCCTCCCTC GCTCGCCGCC TGCTCCTCCT CGGCTCTCCG 
CCTCCTCCCC TCCTTCTCCT CCTCAGCCGC TCCTCTAGCC GCCGCCGCCG CCTCCACAGC 
CTGGGCCTCG CCGCGATGCC GGAGAAGCGG CCCTTCGAGC GGCTGCCCAC CGAGGTGTCG 
CCCATCAACT ACAGCCTCTG CCTCAAGCCC GATTTGCTGG ACTTCACCTT CGAGGGCAAG 
CTGGAGGCCG CCGCCCAGGT AAGGCAGGCA ACCAATCAGA TTGTGATGAA TTGTGCTGAT 
ATTGACATTA TTACAGCTTC ATATGCGCCA GAAGGAGATG AAGAAATCCA TGCGACAGGA 
TTTAACTATC AGAATGAAGA TGAGAAAGTC ACCTTGTCTT TTCCTAGTAC TCTCCAAACA 
GGTACAGGAA CCTTAAAGAT AGATTTTGTT GGAGAACTGA ACGACAAAAT GAAAGGTTTC 
TACAGAAGTA GATATACCAC CCCCGCCGGC GAGGTGCGCT ATGCTGCTGT AACACAGTTT 
GAGGCTACTG ATGCCCGAAG GGCTTTTCCT TGCTGGGATG AGCCTGCTAT CAAAGCAACT 
TTTGATATCT CGTTGGTTGT GCCTAAAGAT AGAGTGGCTT TATCAAATAT GAATGTCATT 
GACAGGAAAC CATACCCTGA TGATGAAAAC TTAGTGGAAG TGAAGTTTGC TCGCACACCT 
GTTATGTCTA CATATCTGGT GGCATTTGTT GTGGGTGAAT ATGACTTTGT AGAAACAAGG 
TCAAAAGATG GTGTGTGTGT CCGTGTTTAC ACCCCTGTTG GCAAAGCAGA GCAAGGAAAA 
TTTGCACTAG AGGTTGCTGC TAAAACCTTG CCTTTTTATA AAGACTACTT CAATGTTCCT 
TATCCTCTAC CTAAAATTGA TCTCATTGCT ATTGCTGACT TTGCAGCTGG TGCCATGGAG 
AACTGGGGCC TTGTTACTTA TAGGGAGACG GCATTGCTTA TTGATCCAAA AAACTCGTGT 
TCTTCATCAC GCCAGTGGGT TGCTCTGGTT GTGGGACATG AACTCGCCCA TCAATGGTTT 
GGAAATCTTG TTACTATGGA ATGGTGGACT CATCTCTGGT TGAACGAAGG CTTTGCATCC 
TGGATTGAGT ACCTTTGTGT AGACCACTGC TTCCCAGAGT ATGATATCTG GACTCAGTTT 
GTTTCTGCAG ATTATACCCG TGCCCAGGAA CTCGATGCCT TAGATAACAG CCATCCTATT 
GAAGTCAGTG TGGGCCATCC ATCTGAGGTT GATGAGATAT TTGATGCTAT ATCATACAGC 
AAAGGTGCAT CTGTAATCCG AATGCTACAT GACTACATTG GCGATAAGGA CTTTAAGAAA 
GGAATGAATA TGTATTTAAC CAAGTTCCAA CAAAAGAATG CTGCCACAGA GGATCTTTGG 
GAAAGTTTGG AAAGTGCCAG TGGCAAACCC ATAGCCGCTG TGATGAACAC CTGGACCAAA 
CAAATGGGGT TCCCTCTCAT TTATGTGGAA GCTGAACAGG TAGAAGATGA CAGAGTGCTG 
AAGCTGTCTC AAAAGAAGTT CTGTGCCAGC GGACCTTATG TCGGTGAAGA CTGTCCTCAG 
TGGATGGTTC CTATCACGAT TTCAACTAGT GAAGATCCCA ACCAGGCTAA ACTGAAAATA 
CTAATGGATA AGCCAGAGAT GAGTGTGGTT TTGAAAAATG TCAAACCAGA CCAATGGGTA 
AAGCTAAACC TGGGAACAGT TGGGTTTTAT CGAACCCAAT ACAGCTCTGC TATGCTGGAA 
AGTTTATTAC CAGGCATTCG TGACCTTTCT CTGCCCCCAG TGGATCGGCT TGGATTACAG 
AATGACCTCT TTTCTCTGGC TCGAGCTGGA ATCATTAGCA CTGTAGAGGT TCTAAAAGTC 
ATGGAGGCTT TTGTGAATGA GCCCAATTAT ACTGTGTGGA GCGACCTGAG CTGTAACCTG 
GGGATTCTTT CAACTCTCTT GTCCCACACA GACTTCTATG AGGAAATCCA GGAGTTTGTC 
AAAGATGTCT TCTCACCTAT AGGAGAGAGA CTGGGCTGGG ACCCCAAACC TGGAGAAGGT 
CATCTAGACG CACTCCTGAG GGGCTTGGTG CTGGGCAAAC TGGGAAAAGC AGGGCATAAG 
GCAACTTTGG AAGAAGCCCG CCGTCGGTTT AAGGAGCACG TGGAAGGGAA GCAGATTCTT 
TCTGCTGACC TAAGGAGTCC TGTTTATCTT ACTGTTTTAA AGCATGGTGA TGGCACTACC 
TTAGACATTA TGCTAAAGCT TCACAAACAA GCTGATATGC AAGAAGAGAA AAACAGAATC 
GAAAGAGTTC TTGGGGCTAC TCTTTCTCCT GAACTGATTC AAAAAGTCCT CACTTTTGCA 
CTTTCAGAAG AGGTACGTCC ACAGGACACT GTGTCAGTAA TTGGTGGAGT GGCTGGAGGC 
AGCAAGCATG GGAGGAAAGC TGCTTGGAAA TTCATAAAGG ACAACTGGGA AGAGCTTTAT 
AATCGATACC AGGGAGGGTT TTTAATATCC AGACTCATAA AGCTGTCAGT TGAGGGGTTT 
GCAGTTGATA AAATGGCTGG AGAAGTTAAG GCTTTCTTCG AGAGTCACCC AGCTCCTTCA 
GCCGAACGTA CCATCCAGCA GTGTTGTGAG AATATCCTGC TGAACGCTGC TTGGCTAAAG 
CGGGATGCTG ACAGCATTCA CCAATACCTC CTGCAGCGGA AAGCCTCCCC TCCCTCGGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Npepps ( XM_017597478.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017452967.1 Rattus norvegicus aminopeptidase puromycin sensitive (Npepps), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa57448
Clone ID Related Accession (Same CDS sequence)	XM_017597478.1
Accession Version	XM_017597478.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2295bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	puromycin-sensitive aminopeptidase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGGTT TCTACAGAAG TAGATATACC ACCCCCGCCG GCGAGGTGCG CTATGCTGCT 
GTAACACAGT TTGAGGCTAC TGATGCCCGA AGGGCTTTTC CTTGCTGGGA TGAGCCTGCT 
ATCAAAGCAA CTTTTGATAT CTCGTTGGTT GTGCCTAAAG ATAGAGTGGC TTTATCAAAT 
ATGAATGTCA TTGACAGGAA ACCATACCCT GATGATGAAA ACTTAGTGGA AGTGAAGTTT 
GCTCGCACAC CTGTTATGTC TACATATCTG GTGGCATTTG TTGTGGGTGA ATATGACTTT 
GTAGAAACAA GGTCAAAAGA TGGTGTGTGT GTCCGTGTTT ACACCCCTGT TGGCAAAGCA 
GAGCAAGGAA AATTTGCACT AGAGGTTGCT GCTAAAACCT TGCCTTTTTA TAAAGACTAC 
TTCAATGTTC CTTATCCTCT ACCTAAAATT GATCTCATTG CTATTGCTGA CTTTGCAGCT 
GGTGCCATGG AGAACTGGGG CCTTGTTACT TATAGGGAGA CGGCATTGCT TATTGATCCA 
AAAAACTCGT GTTCTTCATC ACGCCAGTGG GTTGCTCTGG TTGTGGGACA TGAACTCGCC 
CATCAATGGT TTGGAAATCT TGTTACTATG GAATGGTGGA CTCATCTCTG GTTGAACGAA 
GGCTTTGCAT CCTGGATTGA GTACCTTTGT GTAGACCACT GCTTCCCAGA GTATGATATC 
TGGACTCAGT TTGTTTCTGC AGATTATACC CGTGCCCAGG AACTCGATGC CTTAGATAAC 
AGCCATCCTA TTGAAGTCAG TGTGGGCCAT CCATCTGAGG TTGATGAGAT ATTTGATGCT 
ATATCATACA GCAAAGGTGC ATCTGTAATC CGAATGCTAC ATGACTACAT TGGCGATAAG 
GACTTTAAGA AAGGAATGAA TATGTATTTA ACCAAGTTCC AACAAAAGAA TGCTGCCACA 
GAGGATCTTT GGGAAAGTTT GGAAAGTGCC AGTGGCAAAC CCATAGCCGC TGTGATGAAC 
ACCTGGACCA AACAAATGGG GTTCCCTCTC ATTTATGTGG AAGCTGAACA GGTAGAAGAT 
GACAGAGTGC TGAAGCTGTC TCAAAAGAAG TTCTGTGCCA GCGGACCTTA TGTCGGTGAA 
GACTGTCCTC AGTGGATGGT TCCTATCACG ATTTCAACTA GTGAAGATCC CAACCAGGCT 
AAACTGAAAA TACTAATGGA TAAGCCAGAG ATGAGTGTGG TTTTGAAAAA TGTCAAACCA 
GACCAATGGG TAAAGCTAAA CCTGGGAACA GTTGGGTTTT ATCGAACCCA ATACAGCTCT 
GCTATGCTGG AAAGTTTATT ACCAGGCATT CGTGACCTTT CTCTGCCCCC AGTGGATCGG 
CTTGGATTAC AGAATGACCT CTTTTCTCTG GCTCGAGCTG GAATCATTAG CACTGTAGAG 
GTTCTAAAAG TCATGGAGGC TTTTGTGAAT GAGCCCAATT ATACTGTGTG GAGCGACCTG 
AGCTGTAACC TGGGGATTCT TTCAACTCTC TTGTCCCACA CAGACTTCTA TGAGGAAATC 
CAGGAGTTTG TCAAAGATGT CTTCTCACCT ATAGGAGAGA GACTGGGCTG GGACCCCAAA 
CCTGGAGAAG GTCATCTAGA CGCACTCCTG AGGGGCTTGG TGCTGGGCAA ACTGGGAAAA 
GCAGGGCATA AGGCAACTTT GGAAGAAGCC CGCCGTCGGT TTAAGGAGCA CGTGGAAGGG 
AAGCAGATTC TTTCTGCTGA CCTAAGGAGT CCTGTTTATC TTACTGTTTT AAAGCATGGT 
GATGGCACTA CCTTAGACAT TATGCTAAAG CTTCACAAAC AAGCTGATAT GCAAGAAGAG 
AAAAACAGAA TCGAAAGAGT TCTTGGGGCT ACTCTTTCTC CTGAACTGAT TCAAAAAGTC 
CTCACTTTTG CACTTTCAGA AGAGGTACGT CCACAGGACA CTGTGTCAGT AATTGGTGGA 
GTGGCTGGAG GCAGCAAGCA TGGGAGGAAA GCTGCTTGGA AATTCATAAA GGACAACTGG 
GAAGAGCTTT ATAATCGATA CCAGGGAGGG TTTTTAATAT CCAGACTCAT AAAGCTGTCA 
GTTGAGGGGT TTGCAGTTGA TAAAATGGCT GGAGAAGTTA AGGCTTTCTT CGAGAGTCAC 
CCAGCTCCTT CAGCCGAACG TACCATCCAG CAGTGTTGTG AGAATATCCT GCTGAACGCT 
GCTTGGCTAA AGCGGGATGC TGACAGCATT CACCAATACC TCCTGCAGCG GAAAGCCTCC 
CCTCCCTCGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017452967.1
CDS	350..2644
Translation	MKGFYRSRYTTPAGEVRYAAVTQFEATDARRAFPCWDEPAIKATFDISLVVPKDRVALSNMNVIDRKPYPDDENLVEVKFARTPVMSTYLVAFVVGEYDFVETRSKDGVCVRVYTPVGKAEQGKFALEVAAKTLPFYKDYFNVPYPLPKIDLIAIADFAAGAMENWGLVTYRETALLIDPKNSCSSSRQWVALVVGHELAHQWFGNLVTMEWWTHLWLNEGFASWIEYLCVDHCFPEYDIWTQFVSADYTRAQELDALDNSHPIEVSVGHPSEVDEIFDAISYSKGASVIRMLHDYIGDKDFKKGMNMYLTKFQQKNAATEDLWESLESASGKPIAAVMNTWTKQMGFPLIYVEAEQVEDDRVLKLSQKKFCASGPYVGEDCPQWMVPITISTSEDPNQAKLKILMDKPEMSVVLKNVKPDQWVKLNLGTVGFYRTQYSSAMLESLLPGIRDLSLPPVDRLGLQNDLFSLARAGIISTVEVLKVMEAFVNEPNYTVWSDLSCNLGILSTLLSHTDFYEEIQEFVKDVFSPIGERLGWDPKPGEGHLDALLRGLVLGKLGKAGHKATLEEARRRFKEHVEGKQILSADLRSPVYLTVLKHGDGTTLDIMLKLHKQADMQEEKNRIERVLGATLSPELIQKVLTFALSEEVRPQDTVSVIGGVAGGSKHGRKAAWKFIKDNWEELYNRYQGGFLISRLIKLSVEGFAVDKMAGEVKAFFESHPAPSAERTIQQCCENILLNAAWLKRDADSIHQYLLQRKASPPSV

Target ORF information:

RefSeq Version	XM_017597478.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus aminopeptidase puromycin sensitive (Npepps), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017597478.1

ORF Insert Sequence:

ATGAAAGGTT TCTACAGAAG TAGATATACC ACCCCCGCCG GCGAGGTGCG CTATGCTGCT 
GTAACACAGT TTGAGGCTAC TGATGCCCGA AGGGCTTTTC CTTGCTGGGA TGAGCCTGCT 
ATCAAAGCAA CTTTTGATAT CTCGTTGGTT GTGCCTAAAG ATAGAGTGGC TTTATCAAAT 
ATGAATGTCA TTGACAGGAA ACCATACCCT GATGATGAAA ACTTAGTGGA AGTGAAGTTT 
GCTCGCACAC CTGTTATGTC TACATATCTG GTGGCATTTG TTGTGGGTGA ATATGACTTT 
GTAGAAACAA GGTCAAAAGA TGGTGTGTGT GTCCGTGTTT ACACCCCTGT TGGCAAAGCA 
GAGCAAGGAA AATTTGCACT AGAGGTTGCT GCTAAAACCT TGCCTTTTTA TAAAGACTAC 
TTCAATGTTC CTTATCCTCT ACCTAAAATT GATCTCATTG CTATTGCTGA CTTTGCAGCT 
GGTGCCATGG AGAACTGGGG CCTTGTTACT TATAGGGAGA CGGCATTGCT TATTGATCCA 
AAAAACTCGT GTTCTTCATC ACGCCAGTGG GTTGCTCTGG TTGTGGGACA TGAACTCGCC 
CATCAATGGT TTGGAAATCT TGTTACTATG GAATGGTGGA CTCATCTCTG GTTGAACGAA 
GGCTTTGCAT CCTGGATTGA GTACCTTTGT GTAGACCACT GCTTCCCAGA GTATGATATC 
TGGACTCAGT TTGTTTCTGC AGATTATACC CGTGCCCAGG AACTCGATGC CTTAGATAAC 
AGCCATCCTA TTGAAGTCAG TGTGGGCCAT CCATCTGAGG TTGATGAGAT ATTTGATGCT 
ATATCATACA GCAAAGGTGC ATCTGTAATC CGAATGCTAC ATGACTACAT TGGCGATAAG 
GACTTTAAGA AAGGAATGAA TATGTATTTA ACCAAGTTCC AACAAAAGAA TGCTGCCACA 
GAGGATCTTT GGGAAAGTTT GGAAAGTGCC AGTGGCAAAC CCATAGCCGC TGTGATGAAC 
ACCTGGACCA AACAAATGGG GTTCCCTCTC ATTTATGTGG AAGCTGAACA GGTAGAAGAT 
GACAGAGTGC TGAAGCTGTC TCAAAAGAAG TTCTGTGCCA GCGGACCTTA TGTCGGTGAA 
GACTGTCCTC AGTGGATGGT TCCTATCACG ATTTCAACTA GTGAAGATCC CAACCAGGCT 
AAACTGAAAA TACTAATGGA TAAGCCAGAG ATGAGTGTGG TTTTGAAAAA TGTCAAACCA 
GACCAATGGG TAAAGCTAAA CCTGGGAACA GTTGGGTTTT ATCGAACCCA ATACAGCTCT 
GCTATGCTGG AAAGTTTATT ACCAGGCATT CGTGACCTTT CTCTGCCCCC AGTGGATCGG 
CTTGGATTAC AGAATGACCT CTTTTCTCTG GCTCGAGCTG GAATCATTAG CACTGTAGAG 
GTTCTAAAAG TCATGGAGGC TTTTGTGAAT GAGCCCAATT ATACTGTGTG GAGCGACCTG 
AGCTGTAACC TGGGGATTCT TTCAACTCTC TTGTCCCACA CAGACTTCTA TGAGGAAATC 
CAGGAGTTTG TCAAAGATGT CTTCTCACCT ATAGGAGAGA GACTGGGCTG GGACCCCAAA 
CCTGGAGAAG GTCATCTAGA CGCACTCCTG AGGGGCTTGG TGCTGGGCAA ACTGGGAAAA 
GCAGGGCATA AGGCAACTTT GGAAGAAGCC CGCCGTCGGT TTAAGGAGCA CGTGGAAGGG 
AAGCAGATTC TTTCTGCTGA CCTAAGGAGT CCTGTTTATC TTACTGTTTT AAAGCATGGT 
GATGGCACTA CCTTAGACAT TATGCTAAAG CTTCACAAAC AAGCTGATAT GCAAGAAGAG 
AAAAACAGAA TCGAAAGAGT TCTTGGGGCT ACTCTTTCTC CTGAACTGAT TCAAAAAGTC 
CTCACTTTTG CACTTTCAGA AGAGGTACGT CCACAGGACA CTGTGTCAGT AATTGGTGGA 
GTGGCTGGAG GCAGCAAGCA TGGGAGGAAA GCTGCTTGGA AATTCATAAA GGACAACTGG 
GAAGAGCTTT ATAATCGATA CCAGGGAGGG TTTTTAATAT CCAGACTCAT AAAGCTGTCA 
GTTGAGGGGT TTGCAGTTGA TAAAATGGCT GGAGAAGTTA AGGCTTTCTT CGAGAGTCAC 
CCAGCTCCTT CAGCCGAACG TACCATCCAG CAGTGTTGTG AGAATATCCT GCTGAACGCT 
GCTTGGCTAA AGCGGGATGC TGACAGCATT CACCAATACC TCCTGCAGCG GAAAGCCTCC 
CCTCCCTCGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-content genome-wide RNAi screens identify regulators of parkin upstream of mitophagy.
Nature504(7479)291-5(2013 Dec)
Hasson SA,Kane LA,Yamano K,Huang CH,Sliter DA,Buehler E,Wang C,Heman-Ackah SM,Hessa T,Guha R,Martin SE,Youle RJ

Hypoxia enhances transcriptional activity of androgen receptor through hypoxia-inducible factor-1α in a low androgen environment.
The Journal of steroid biochemistry and molecular biology123(1-2)58-64(2011 Jan)
Mitani T,Yamaji R,Higashimura Y,Harada N,Nakano Y,Inui H

MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis.
Immunology and cell biology88(8)851-6(2010 Nov-Dec)
Buschow SI,van Balkom BW,Aalberts M,Heck AJ,Wauben M,Stoorvogel W

Large-scale proteomics and phosphoproteomics of urinary exosomes.
Journal of the American Society of Nephrology : JASN20(2)363-79(2009 Feb)
Gonzales PA,Pisitkun T,Hoffert JD,Tchapyjnikov D,Star RA,Kleta R,Wang NS,Knepper MA

Gene expression profile of the rat eye iridocorneal angle: NEIBank expressed sequence tag analysis.
Investigative ophthalmology & visual science45(9)3081-90(2004 Sep)
Ahmed F,Torrado M,Zinovieva RD,Senatorov VV,Wistow G,Tomarev SI

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Npepps cDNA ORF clone, Rattus norvegicus(Norway rat)

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gRNAs

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