Misp cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Misp
Entrez Gene ID	500797
Full Name	mitotic spindle positioning
General protein information	Preferred Names mitotic spindle positioning Names mitotic interactor and substrate of PLK1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q11

mRNA and Protein(s)

mRNA	Protein	Name
XM_017595043.1	XP_017450532.1	mitotic interactor and substrate of PLK1 isoform X1
XM_017595044.1	XP_017450533.1	mitotic interactor and substrate of PLK1 isoform X1
XM_017595045.1	XP_017450534.1	mitotic interactor and substrate of PLK1 isoform X2
NM_001109284.1	NP_001102754.1	mitotic interactor and substrate of PLK1

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	C28H19ORF21	XP_418218.4
Pan troglodytes (chimpanzee)	MISP	XP_001146667.1
Bos taurus (cattle)	MISP	NP_001030346.1
Xenopus tropicalis (tropical clawed frog)	misp	XP_002937429.1
Homo sapiens (human)	MISP	NP_775752.1
Mus musculus (house mouse)	Misp	NP_084494.1
Rattus norvegicus (Norway rat)	Misp	NP_001102754.1

Gene Ontology
Bibliography

Related articles in PubMed

The novel actin/focal adhesion-associated protein MISP is involved in mitotic spindle positioning in human cells.
Maier B, Kirsch M, Anderhub S, Zentgraf H, Krämer A
Cell cycle (Georgetown, Tex.)12(9)1457-71(2013 May)

MISP is a novel Plk1 substrate required for proper spindle orientation and mitotic progression.
Zhu M, Settele F, Kotak S, Sanchez-Pulido L, Ehret L, Ponting CP, Gönczy P, Hoffmann I
The Journal of cell biology200(6)773-87(2013 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Misp gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Misp cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa09531	NM_001109284.1 Latest version!	Rattus norvegicus mitotic spindle positioning (Misp), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ORa09531	XM_017595045.1 Latest version!	Rattus norvegicus mitotic spindle positioning (Misp), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ORa62122	XM_017595044.1 Latest version!	Rattus norvegicus mitotic spindle positioning (Misp), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ORa62122	XM_017595043.1 Latest version!	Rattus norvegicus mitotic spindle positioning (Misp), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Misp ( NM_001109284.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001102754.1 Rattus norvegicus mitotic spindle positioning (Misp), mRNA.

Price & Availability↑

CloneID	ORa09531
Clone ID Related Accession (Same CDS sequence)	XM_017595045.1 , NM_001109284.1
Accession Version	NM_001109284.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1570636800000
Organism	Rattus norvegicus(Norway rat)
Product	mitotic interactor and substrate of PLK1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH474029.1. On or before Oct 4, 2007 this sequence version replaced XM_576186.2, XM_001077148.1. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2689602, SAMN02642680 [ECO:0000348] ##Evidence-Data-END##

ATGGACCGAG TGACCAGATA CCCCATCTTC AGCAAGCCCC ACTCAGCACG TGTCACCGGC 
TTGGCCTTGG ATGAAGACAC CAGCTACACC GTTGAGCTAG TGGGCGTAGG GCCTGAGGCT 
GGTTGGAGCC AGGGTGATCT GCAAACCTGG TCCGCTGAGG ACCAGACCAA GCCAGATGTG 
AAGAGAGTAA GCGTGTCTTC CACCAGACGA GCCTTCCTGG GGCAGCAGTC CCCGAGGTCA 
CTGTACTGGG AAGATGAGGA CGAGGAGACA AAAGCTTACC GCGTGGATGC CGGCAGCAAT 
GCCTTCTGCA GGCAGCCGAG GGACCTGGAG GCCGAGCGCT GGGCAGTCAT CCAGAGCCAG 
GCGGTCAGGA AGGGTGGCAC AGTGGCCACA CTCCAGGCTT CATCAGACCA TAGGGACCCC 
AGGATCGCCA GCCATCCCCG GTCTTCATTC ACAGAGGAGA TCTTGGTGGA CACGGAGCAA 
ATCGATTTCC TGGCTGCTCG GCAGCAGTTC CTGAGTTTAG AGAAGGCAAA CACAAATCCG 
GTCACGTGGA GCCCCACAGC CAGGGAGACC TTTGCACGTA CCCCACCTGG CGTCGACCAG 
ACCCCCAAGG CCTCCACTGG GCCCCATGTA GCCAATGGCT ACACCACTTC AGTCACGTCA 
CCAATGAAGG AAATGACCTT GGAGAAGACT TTCCATTTCC ACACTTCCCC AGCCGGGTCC 
ATCCGTACAA CGGATGACCC TGGCCACCAG ACCCGAGCAG AGTCCCCTGA GACCCCCAAG 
GAGACGCCCA TCGAGAGAGA GATCAGACTG GCCCAGGAGC GAGAGGCGGA ACTGCGGGAG 
CAGAGAGGGC TTGGGCGGGC TGCCGGGCAT CAAGAGCTCA TACAGATCCC CAGCAGGCCC 
CTGCTCAACA AGATGAGCCT GACAGAGACA CCCCCACGAA GAGACAGGGG CCGCCCATCC 
CTCTACGTGC AGAGGGACAT GGTGCAAGAA ACGCAACGAG AAGAAGATCA TCGCCGAGAG 
GGTCTGCAGG TGGGCAGGGC GTCCACACCT GACTGGACTT CCCAGGACCC TCAGCCTGGG 
TTCCAGAGAA GCCTGAGCTC CGACTGCATC CTCAGCCCAG ATGCCCGAGC TGCAGACCCA 
GCTCCAGAGG CAAGGAAAGT CAACCGGATC CCACCAGATG CCTACCAGCC ATACCTGGGC 
CCTGGGACCC CCAAATTAGA ATTCTCAGCC TTCGGAGTAT ACAGCAAGCC AAGTGGTGTC 
CACTCCACAG AGGACACCAA AGCCACAGCC TTCCGGAACA CCACAGAGTC TCCAAGGCAT 
GTTTCAGAAT CCTCCAGAAG ATCTCTGAGC TCAAAACAAG AGTGGTCCAA GCCTCCTGGG 
AAGCCTGCCA ACGGGGGTGT TGTGAAATTA GAGAATTTCC ACCTGCGTCC ACTGCGATTC 
AAGGTCCCAG ATGTCCCCCA GGAAGCTGAG ACCCCCCAAA CCTGGGGCTG GGAAGTGGCC 
GGAGGCCCTG TGTTGAGACT ACAAAAGTCA CAGTCATCTG ACCTGCTGGA GAGGGAGGTG 
GAGAGCGTCC TGCGGAGGGA GCGAGAGGTA GCTGAAGAGC GGAGGAATGC CTTCTTCCCA 
GAGGTATTCT CCCCAGTGCT GGACCAGGAA GAGAGCCATG AGCAAGACTC CCGCAGTTCC 
TCTCGGGCAT CTGGCATCAC CGGCAGCTAT TCCGTGTCAG AATCACCATT ATTCACGCCC 
ATTCACCTGC ATTCCGGCCT GGTGTGGAAG GTGGAGGCCC CAGAGGACAG TTCTCCGCCA 
GGGCAGAAGA CTAGAAAGGA GTCGTGGTAT GCAGGTATCA ACCCCTCAGA CGGTGTCAAC 
TCGGAGGTCC TGGGAGCCAC GAGGGTAAAG CGACACAAGA ACCTCTTGGC GGAACGCTGG 
GAAGCACATA TCTATGCCAG CGAGGATGAG AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001102754.1
CDS	146..2101
Translation	MDRVTRYPIFSKPHSARVTGLALDEDTSYTVELVGVGPEAGWSQGDLQTWSAEDQTKPDVKRVSVSSTRRAFLGQQSPRSLYWEDEDEETKAYRVDAGSNAFCRQPRDLEAERWAVIQSQAVRKGGTVATLQASSDHRDPRIASHPRSSFTEEILVDTEQIDFLAARQQFLSLEKANTNPVTWSPTARETFARTPPGVDQTPKASTGPHVANGYTTSVTSPMKEMTLEKTFHFHTSPAGSIRTTDDPGHQTRAESPETPKETPIEREIRLAQEREAELREQRGLGRAAGHQELIQIPSRPLLNKMSLTETPPRRDRGRPSLYVQRDMVQETQREEDHRREGLQVGRASTPDWTSQDPQPGFQRSLSSDCILSPDARAADPAPEARKVNRIPPDAYQPYLGPGTPKLEFSAFGVYSKPSGVHSTEDTKATAFRNTTESPRHVSESSRRSLSSKQEWSKPPGKPANGGVVKLENFHLRPLRFKVPDVPQEAETPQTWGWEVAGGPVLRLQKSQSSDLLEREVESVLRREREVAEERRNAFFPEVFSPVLDQEESHEQDSRSSSRASGITGSYSVSESPLFTPIHLHSGLVWKVEAPEDSSPPGQKTRKESWYAGINPSDGVNSEVLGATRVKRHKNLLAERWEAHIYASEDEN

Target ORF information:

RefSeq Version	NM_001109284.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mitotic spindle positioning (Misp), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001109284.1

ORF Insert Sequence:

ATGGACCGAG TGACCAGATA CCCCATCTTC AGCAAGCCCC ACTCAGCACG TGTCACCGGC 
TTGGCCTTGG ATGAAGACAC CAGCTACACC GTTGAGCTAG TGGGCGTAGG GCCTGAGGCT 
GGTTGGAGCC AGGGTGATCT GCAAACCTGG TCCGCTGAGG ACCAGACCAA GCCAGATGTG 
AAGAGAGTAA GCGTGTCTTC CACCAGACGA GCCTTCCTGG GGCAGCAGTC CCCGAGGTCA 
CTGTACTGGG AAGATGAGGA CGAGGAGACA AAAGCTTACC GCGTGGATGC CGGCAGCAAT 
GCCTTCTGCA GGCAGCCGAG GGACCTGGAG GCCGAGCGCT GGGCAGTCAT CCAGAGCCAG 
GCGGTCAGGA AGGGTGGCAC AGTGGCCACA CTCCAGGCTT CATCAGACCA TAGGGACCCC 
AGGATCGCCA GCCATCCCCG GTCTTCATTC ACAGAGGAGA TCTTGGTGGA CACGGAGCAA 
ATCGATTTCC TGGCTGCTCG GCAGCAGTTC CTGAGTTTAG AGAAGGCAAA CACAAATCCG 
GTCACGTGGA GCCCCACAGC CAGGGAGACC TTTGCACGTA CCCCACCTGG CGTCGACCAG 
ACCCCCAAGG CCTCCACTGG GCCCCATGTA GCCAATGGCT ACACCACTTC AGTCACGTCA 
CCAATGAAGG AAATGACCTT GGAGAAGACT TTCCATTTCC ACACTTCCCC AGCCGGGTCC 
ATCCGTACAA CGGATGACCC TGGCCACCAG ACCCGAGCAG AGTCCCCTGA GACCCCCAAG 
GAGACGCCCA TCGAGAGAGA GATCAGACTG GCCCAGGAGC GAGAGGCGGA ACTGCGGGAG 
CAGAGAGGGC TTGGGCGGGC TGCCGGGCAT CAAGAGCTCA TACAGATCCC CAGCAGGCCC 
CTGCTCAACA AGATGAGCCT GACAGAGACA CCCCCACGAA GAGACAGGGG CCGCCCATCC 
CTCTACGTGC AGAGGGACAT GGTGCAAGAA ACGCAACGAG AAGAAGATCA TCGCCGAGAG 
GGTCTGCAGG TGGGCAGGGC GTCCACACCT GACTGGACTT CCCAGGACCC TCAGCCTGGG 
TTCCAGAGAA GCCTGAGCTC CGACTGCATC CTCAGCCCAG ATGCCCGAGC TGCAGACCCA 
GCTCCAGAGG CAAGGAAAGT CAACCGGATC CCACCAGATG CCTACCAGCC ATACCTGGGC 
CCTGGGACCC CCAAATTAGA ATTCTCAGCC TTCGGAGTAT ACAGCAAGCC AAGTGGTGTC 
CACTCCACAG AGGACACCAA AGCCACAGCC TTCCGGAACA CCACAGAGTC TCCAAGGCAT 
GTTTCAGAAT CCTCCAGAAG ATCTCTGAGC TCAAAACAAG AGTGGTCCAA GCCTCCTGGG 
AAGCCTGCCA ACGGGGGTGT TGTGAAATTA GAGAATTTCC ACCTGCGTCC ACTGCGATTC 
AAGGTCCCAG ATGTCCCCCA GGAAGCTGAG ACCCCCCAAA CCTGGGGCTG GGAAGTGGCC 
GGAGGCCCTG TGTTGAGACT ACAAAAGTCA CAGTCATCTG ACCTGCTGGA GAGGGAGGTG 
GAGAGCGTCC TGCGGAGGGA GCGAGAGGTA GCTGAAGAGC GGAGGAATGC CTTCTTCCCA 
GAGGTATTCT CCCCAGTGCT GGACCAGGAA GAGAGCCATG AGCAAGACTC CCGCAGTTCC 
TCTCGGGCAT CTGGCATCAC CGGCAGCTAT TCCGTGTCAG AATCACCATT ATTCACGCCC 
ATTCACCTGC ATTCCGGCCT GGTGTGGAAG GTGGAGGCCC CAGAGGACAG TTCTCCGCCA 
GGGCAGAAGA CTAGAAAGGA GTCGTGGTAT GCAGGTATCA ACCCCTCAGA CGGTGTCAAC 
TCGGAGGTCC TGGGAGCCAC GAGGGTAAAG CGACACAAGA ACCTCTTGGC GGAACGCTGG 
GAAGCACATA TCTATGCCAG CGAGGATGAG AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Misp ( XM_017595045.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017450534.1 Rattus norvegicus mitotic spindle positioning (Misp), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa09531
Clone ID Related Accession (Same CDS sequence)	XM_017595045.1 , NM_001109284.1
Accession Version	XM_017595045.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	mitotic interactor and substrate of PLK1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCGAG TGACCAGATA CCCCATCTTC AGCAAGCCCC ACTCAGCACG TGTCACCGGC 
TTGGCCTTGG ATGAAGACAC CAGCTACACC GTTGAGCTAG TGGGCGTAGG GCCTGAGGCT 
GGTTGGAGCC AGGGTGATCT GCAAACCTGG TCCGCTGAGG ACCAGACCAA GCCAGATGTG 
AAGAGAGTAA GCGTGTCTTC CACCAGACGA GCCTTCCTGG GGCAGCAGTC CCCGAGGTCA 
CTGTACTGGG AAGATGAGGA CGAGGAGACA AAAGCTTACC GCGTGGATGC CGGCAGCAAT 
GCCTTCTGCA GGCAGCCGAG GGACCTGGAG GCCGAGCGCT GGGCAGTCAT CCAGAGCCAG 
GCGGTCAGGA AGGGTGGCAC AGTGGCCACA CTCCAGGCTT CATCAGACCA TAGGGACCCC 
AGGATCGCCA GCCATCCCCG GTCTTCATTC ACAGAGGAGA TCTTGGTGGA CACGGAGCAA 
ATCGATTTCC TGGCTGCTCG GCAGCAGTTC CTGAGTTTAG AGAAGGCAAA CACAAATCCG 
GTCACGTGGA GCCCCACAGC CAGGGAGACC TTTGCACGTA CCCCACCTGG CGTCGACCAG 
ACCCCCAAGG CCTCCACTGG GCCCCATGTA GCCAATGGCT ACACCACTTC AGTCACGTCA 
CCAATGAAGG AAATGACCTT GGAGAAGACT TTCCATTTCC ACACTTCCCC AGCCGGGTCC 
ATCCGTACAA CGGATGACCC TGGCCACCAG ACCCGAGCAG AGTCCCCTGA GACCCCCAAG 
GAGACGCCCA TCGAGAGAGA GATCAGACTG GCCCAGGAGC GAGAGGCGGA ACTGCGGGAG 
CAGAGAGGGC TTGGGCGGGC TGCCGGGCAT CAAGAGCTCA TACAGATCCC CAGCAGGCCC 
CTGCTCAACA AGATGAGCCT GACAGAGACA CCCCCACGAA GAGACAGGGG CCGCCCATCC 
CTCTACGTGC AGAGGGACAT GGTGCAAGAA ACGCAACGAG AAGAAGATCA TCGCCGAGAG 
GGTCTGCAGG TGGGCAGGGC GTCCACACCT GACTGGACTT CCCAGGACCC TCAGCCTGGG 
TTCCAGAGAA GCCTGAGCTC CGACTGCATC CTCAGCCCAG ATGCCCGAGC TGCAGACCCA 
GCTCCAGAGG CAAGGAAAGT CAACCGGATC CCACCAGATG CCTACCAGCC ATACCTGGGC 
CCTGGGACCC CCAAATTAGA ATTCTCAGCC TTCGGAGTAT ACAGCAAGCC AAGTGGTGTC 
CACTCCACAG AGGACACCAA AGCCACAGCC TTCCGGAACA CCACAGAGTC TCCAAGGCAT 
GTTTCAGAAT CCTCCAGAAG ATCTCTGAGC TCAAAACAAG AGTGGTCCAA GCCTCCTGGG 
AAGCCTGCCA ACGGGGGTGT TGTGAAATTA GAGAATTTCC ACCTGCGTCC ACTGCGATTC 
AAGGTCCCAG ATGTCCCCCA GGAAGCTGAG ACCCCCCAAA CCTGGGGCTG GGAAGTGGCC 
GGAGGCCCTG TGTTGAGACT ACAAAAGTCA CAGTCATCTG ACCTGCTGGA GAGGGAGGTG 
GAGAGCGTCC TGCGGAGGGA GCGAGAGGTA GCTGAAGAGC GGAGGAATGC CTTCTTCCCA 
GAGGTATTCT CCCCAGTGCT GGACCAGGAA GAGAGCCATG AGCAAGACTC CCGCAGTTCC 
TCTCGGGCAT CTGGCATCAC CGGCAGCTAT TCCGTGTCAG AATCACCATT ATTCACGCCC 
ATTCACCTGC ATTCCGGCCT GGTGTGGAAG GTGGAGGCCC CAGAGGACAG TTCTCCGCCA 
GGGCAGAAGA CTAGAAAGGA GTCGTGGTAT GCAGGTATCA ACCCCTCAGA CGGTGTCAAC 
TCGGAGGTCC TGGGAGCCAC GAGGGTAAAG CGACACAAGA ACCTCTTGGC GGAACGCTGG 
GAAGCACATA TCTATGCCAG CGAGGATGAG AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017450534.1
CDS	226..2181
Translation	MDRVTRYPIFSKPHSARVTGLALDEDTSYTVELVGVGPEAGWSQGDLQTWSAEDQTKPDVKRVSVSSTRRAFLGQQSPRSLYWEDEDEETKAYRVDAGSNAFCRQPRDLEAERWAVIQSQAVRKGGTVATLQASSDHRDPRIASHPRSSFTEEILVDTEQIDFLAARQQFLSLEKANTNPVTWSPTARETFARTPPGVDQTPKASTGPHVANGYTTSVTSPMKEMTLEKTFHFHTSPAGSIRTTDDPGHQTRAESPETPKETPIEREIRLAQEREAELREQRGLGRAAGHQELIQIPSRPLLNKMSLTETPPRRDRGRPSLYVQRDMVQETQREEDHRREGLQVGRASTPDWTSQDPQPGFQRSLSSDCILSPDARAADPAPEARKVNRIPPDAYQPYLGPGTPKLEFSAFGVYSKPSGVHSTEDTKATAFRNTTESPRHVSESSRRSLSSKQEWSKPPGKPANGGVVKLENFHLRPLRFKVPDVPQEAETPQTWGWEVAGGPVLRLQKSQSSDLLEREVESVLRREREVAEERRNAFFPEVFSPVLDQEESHEQDSRSSSRASGITGSYSVSESPLFTPIHLHSGLVWKVEAPEDSSPPGQKTRKESWYAGINPSDGVNSEVLGATRVKRHKNLLAERWEAHIYASEDEN

Target ORF information:

RefSeq Version	XM_017595045.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mitotic spindle positioning (Misp), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017595045.1

ORF Insert Sequence:

ATGGACCGAG TGACCAGATA CCCCATCTTC AGCAAGCCCC ACTCAGCACG TGTCACCGGC 
TTGGCCTTGG ATGAAGACAC CAGCTACACC GTTGAGCTAG TGGGCGTAGG GCCTGAGGCT 
GGTTGGAGCC AGGGTGATCT GCAAACCTGG TCCGCTGAGG ACCAGACCAA GCCAGATGTG 
AAGAGAGTAA GCGTGTCTTC CACCAGACGA GCCTTCCTGG GGCAGCAGTC CCCGAGGTCA 
CTGTACTGGG AAGATGAGGA CGAGGAGACA AAAGCTTACC GCGTGGATGC CGGCAGCAAT 
GCCTTCTGCA GGCAGCCGAG GGACCTGGAG GCCGAGCGCT GGGCAGTCAT CCAGAGCCAG 
GCGGTCAGGA AGGGTGGCAC AGTGGCCACA CTCCAGGCTT CATCAGACCA TAGGGACCCC 
AGGATCGCCA GCCATCCCCG GTCTTCATTC ACAGAGGAGA TCTTGGTGGA CACGGAGCAA 
ATCGATTTCC TGGCTGCTCG GCAGCAGTTC CTGAGTTTAG AGAAGGCAAA CACAAATCCG 
GTCACGTGGA GCCCCACAGC CAGGGAGACC TTTGCACGTA CCCCACCTGG CGTCGACCAG 
ACCCCCAAGG CCTCCACTGG GCCCCATGTA GCCAATGGCT ACACCACTTC AGTCACGTCA 
CCAATGAAGG AAATGACCTT GGAGAAGACT TTCCATTTCC ACACTTCCCC AGCCGGGTCC 
ATCCGTACAA CGGATGACCC TGGCCACCAG ACCCGAGCAG AGTCCCCTGA GACCCCCAAG 
GAGACGCCCA TCGAGAGAGA GATCAGACTG GCCCAGGAGC GAGAGGCGGA ACTGCGGGAG 
CAGAGAGGGC TTGGGCGGGC TGCCGGGCAT CAAGAGCTCA TACAGATCCC CAGCAGGCCC 
CTGCTCAACA AGATGAGCCT GACAGAGACA CCCCCACGAA GAGACAGGGG CCGCCCATCC 
CTCTACGTGC AGAGGGACAT GGTGCAAGAA ACGCAACGAG AAGAAGATCA TCGCCGAGAG 
GGTCTGCAGG TGGGCAGGGC GTCCACACCT GACTGGACTT CCCAGGACCC TCAGCCTGGG 
TTCCAGAGAA GCCTGAGCTC CGACTGCATC CTCAGCCCAG ATGCCCGAGC TGCAGACCCA 
GCTCCAGAGG CAAGGAAAGT CAACCGGATC CCACCAGATG CCTACCAGCC ATACCTGGGC 
CCTGGGACCC CCAAATTAGA ATTCTCAGCC TTCGGAGTAT ACAGCAAGCC AAGTGGTGTC 
CACTCCACAG AGGACACCAA AGCCACAGCC TTCCGGAACA CCACAGAGTC TCCAAGGCAT 
GTTTCAGAAT CCTCCAGAAG ATCTCTGAGC TCAAAACAAG AGTGGTCCAA GCCTCCTGGG 
AAGCCTGCCA ACGGGGGTGT TGTGAAATTA GAGAATTTCC ACCTGCGTCC ACTGCGATTC 
AAGGTCCCAG ATGTCCCCCA GGAAGCTGAG ACCCCCCAAA CCTGGGGCTG GGAAGTGGCC 
GGAGGCCCTG TGTTGAGACT ACAAAAGTCA CAGTCATCTG ACCTGCTGGA GAGGGAGGTG 
GAGAGCGTCC TGCGGAGGGA GCGAGAGGTA GCTGAAGAGC GGAGGAATGC CTTCTTCCCA 
GAGGTATTCT CCCCAGTGCT GGACCAGGAA GAGAGCCATG AGCAAGACTC CCGCAGTTCC 
TCTCGGGCAT CTGGCATCAC CGGCAGCTAT TCCGTGTCAG AATCACCATT ATTCACGCCC 
ATTCACCTGC ATTCCGGCCT GGTGTGGAAG GTGGAGGCCC CAGAGGACAG TTCTCCGCCA 
GGGCAGAAGA CTAGAAAGGA GTCGTGGTAT GCAGGTATCA ACCCCTCAGA CGGTGTCAAC 
TCGGAGGTCC TGGGAGCCAC GAGGGTAAAG CGACACAAGA ACCTCTTGGC GGAACGCTGG 
GAAGCACATA TCTATGCCAG CGAGGATGAG AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Misp ( XM_017595043.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017450532.1 Rattus norvegicus mitotic spindle positioning (Misp), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa62122
Clone ID Related Accession (Same CDS sequence)	XM_017595043.1 , XM_017595044.1
Accession Version	XM_017595043.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2022bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	mitotic interactor and substrate of PLK1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCGAG TGACCAGATA CCCCATCTTC AGCAAGCCCC ACTCAGCACG TGTCACCGGC 
TTGGCCTTGG ATGAAGACAC CAGCTACACC GTTGAGCTAG TGGGCGTAGG GCCTGAGGCT 
GGTTGGAGCC AGGGTGATCT GCAAACCTGG TCCGCTGAGG ACCAGACCAA GCCAGATGTG 
AAGAGAGTAA GCGTGTCTTC CACCAGACGA GCCTTCCTGG GGCAGCAGTC CCCGAGGTCA 
CTGTACTGGG AAGATGAGGA CGAGGAGACA AAAGCTTACC GCGTGGATGC CGGCAGCAAT 
GCCTTCTGCA GGCAGCCGAG GGACCTGGAG GCCGAGCGCT GGGCAGTCAT CCAGAGCCAG 
GCGGTCAGGA AGGGTGGCAC AGTGGCCACA CTCCAGGCTT CATCAGACCA TAGGGACCCC 
AGGATCGCCA GCCATCCCCG GTCTTCATTC ACAGAGGAGA TCTTGGTGGA CACGGAGCAA 
ATCGATTTCC TGGCTGCTCG GCAGCAGTTC CTGAGTTTAG AGAAGGCAAA CACAAATCCG 
GTCACGTGGA GCCCCACAGC CAGGGAGACC TTTGCACGTA CCCCACCTGG CGTCGACCAG 
ACCCCCAAGG CCTCCACTGG GCCCCATGTA GCCAATGGCT ACACCACTTC AGTCACGTCA 
CCAATGAAGG AAATGACCTT GGAGAAGACT TTCCATTTCC ACACTTCCCC AGCCGGGTCC 
ATCCGTACAA CGGATGACCC TGGCCACCAG ACCCGAGCAG AGTCCCCTGA GACCCCCAAG 
GAGACGCCCA TCGAGAGAGA GATCAGACTG GCCCAGGAGC GAGAGGCGGA ACTGCGGGAG 
CAGAGAGGGC TTGGGCGGGC TGCCGGGCAT CAAGAGCTCA TACAGATCCC CAGCAGGCCC 
CTGCTCAACA AGATGAGCCT GACAGAGACA CCCCCACGAA GAGACAGGGG CCGCCCATCC 
CTCTACGTGC AGAGGGACAT GGTGCAAGAA ACGCAACGAG AAGAAGATCA TCGCCGAGAG 
GGTCTGCAGG TGGGCAGGGC GTCCACACCT GACTGGACTT CCCAGGACCC TCAGCCTGGG 
TTCCAGAGAA GCCTGAGCTC CGACTGCATC CTCAGCCCAG ATGCCCGAGC TGCAGACCCA 
GCTCCAGAGG CAAGGAAAGT CAACCGGATC CCACCAGATG CCTACCAGCC ATACCTGGGC 
CCTGGGACCC CCAAATTAGA ATTCTCAGCC TTCGGAGTAT ACAGCAAGCC AAGTGGTGTC 
CACTCCACAG AGGACACCAA AGCCACAGCC TTCCGGAACA CCACAGAGTC TCCAAGGCAT 
GTTTCAGAAT CCTCCAGAAG ATCTCTGAGC TCAAAACAAG AGTGGTCCAA GCCTCCTGGG 
AAGCCTGCCA ACGGGGGTGT TGTGAAATTA GAGAATTTCC ACCTGCGTCC ACTGCGATTC 
AAGGTCCCAG ATGTCCCCCA GGAAGCTGAG ACCCCCCAAA CCTGGGGCTG GGAAGTGGCC 
GGAGGCCCTG TGTTGAGACT ACAAAAGTCA CAGTCATCTG ACCTGCTGGA GAGGGAGGTG 
GAGAGCGTCC TGCGGAGGGA GCGAGAGGTA GCTGAAGAGC GGAGGAATGC CTTCTTCCCA 
GAGGTATTCT CCCCAGTGCT GGACCAGGAA GAGAGCCATG AGCAAGACTC CCGCAGTTCC 
TCTCGGGCAT CTGGCATCAC CGGCAGCTAT TCCGTGTCAG AATCACCATT ATTCACGCCC 
ATTCACCTGC ATTCCGGCCT GGTGTGGAAG GTGGAGGCCC CAGAGGACAG TTCTCCGCCA 
GGGCAGAAGA CTAGAAAGGA GTCGTGGTAT GCAGGTATCA ACCCCTCAGA CGGTGTCAAC 
TCGGAGGAGC TGGGAGTTCT CCATCTCGAT CGGAAGGCAG CTGAACAGGT CTTTCTGGTT 
TCCGCCTATG TGTTCCAAGC AGTGGAAGAG CACCCTACAG CGATATGTGA TAACCCTCCT 
CTCTTAACTT CTTGGCTCCC ACCTTATGAA TGTCAAAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017450532.1
CDS	837..2858
Translation	MDRVTRYPIFSKPHSARVTGLALDEDTSYTVELVGVGPEAGWSQGDLQTWSAEDQTKPDVKRVSVSSTRRAFLGQQSPRSLYWEDEDEETKAYRVDAGSNAFCRQPRDLEAERWAVIQSQAVRKGGTVATLQASSDHRDPRIASHPRSSFTEEILVDTEQIDFLAARQQFLSLEKANTNPVTWSPTARETFARTPPGVDQTPKASTGPHVANGYTTSVTSPMKEMTLEKTFHFHTSPAGSIRTTDDPGHQTRAESPETPKETPIEREIRLAQEREAELREQRGLGRAAGHQELIQIPSRPLLNKMSLTETPPRRDRGRPSLYVQRDMVQETQREEDHRREGLQVGRASTPDWTSQDPQPGFQRSLSSDCILSPDARAADPAPEARKVNRIPPDAYQPYLGPGTPKLEFSAFGVYSKPSGVHSTEDTKATAFRNTTESPRHVSESSRRSLSSKQEWSKPPGKPANGGVVKLENFHLRPLRFKVPDVPQEAETPQTWGWEVAGGPVLRLQKSQSSDLLEREVESVLRREREVAEERRNAFFPEVFSPVLDQEESHEQDSRSSSRASGITGSYSVSESPLFTPIHLHSGLVWKVEAPEDSSPPGQKTRKESWYAGINPSDGVNSEELGVLHLDRKAAEQVFLVSAYVFQAVEEHPTAICDNPPLLTSWLPPYECQS

Target ORF information:

RefSeq Version	XM_017595043.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mitotic spindle positioning (Misp), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017595043.1

ORF Insert Sequence:

ATGGACCGAG TGACCAGATA CCCCATCTTC AGCAAGCCCC ACTCAGCACG TGTCACCGGC 
TTGGCCTTGG ATGAAGACAC CAGCTACACC GTTGAGCTAG TGGGCGTAGG GCCTGAGGCT 
GGTTGGAGCC AGGGTGATCT GCAAACCTGG TCCGCTGAGG ACCAGACCAA GCCAGATGTG 
AAGAGAGTAA GCGTGTCTTC CACCAGACGA GCCTTCCTGG GGCAGCAGTC CCCGAGGTCA 
CTGTACTGGG AAGATGAGGA CGAGGAGACA AAAGCTTACC GCGTGGATGC CGGCAGCAAT 
GCCTTCTGCA GGCAGCCGAG GGACCTGGAG GCCGAGCGCT GGGCAGTCAT CCAGAGCCAG 
GCGGTCAGGA AGGGTGGCAC AGTGGCCACA CTCCAGGCTT CATCAGACCA TAGGGACCCC 
AGGATCGCCA GCCATCCCCG GTCTTCATTC ACAGAGGAGA TCTTGGTGGA CACGGAGCAA 
ATCGATTTCC TGGCTGCTCG GCAGCAGTTC CTGAGTTTAG AGAAGGCAAA CACAAATCCG 
GTCACGTGGA GCCCCACAGC CAGGGAGACC TTTGCACGTA CCCCACCTGG CGTCGACCAG 
ACCCCCAAGG CCTCCACTGG GCCCCATGTA GCCAATGGCT ACACCACTTC AGTCACGTCA 
CCAATGAAGG AAATGACCTT GGAGAAGACT TTCCATTTCC ACACTTCCCC AGCCGGGTCC 
ATCCGTACAA CGGATGACCC TGGCCACCAG ACCCGAGCAG AGTCCCCTGA GACCCCCAAG 
GAGACGCCCA TCGAGAGAGA GATCAGACTG GCCCAGGAGC GAGAGGCGGA ACTGCGGGAG 
CAGAGAGGGC TTGGGCGGGC TGCCGGGCAT CAAGAGCTCA TACAGATCCC CAGCAGGCCC 
CTGCTCAACA AGATGAGCCT GACAGAGACA CCCCCACGAA GAGACAGGGG CCGCCCATCC 
CTCTACGTGC AGAGGGACAT GGTGCAAGAA ACGCAACGAG AAGAAGATCA TCGCCGAGAG 
GGTCTGCAGG TGGGCAGGGC GTCCACACCT GACTGGACTT CCCAGGACCC TCAGCCTGGG 
TTCCAGAGAA GCCTGAGCTC CGACTGCATC CTCAGCCCAG ATGCCCGAGC TGCAGACCCA 
GCTCCAGAGG CAAGGAAAGT CAACCGGATC CCACCAGATG CCTACCAGCC ATACCTGGGC 
CCTGGGACCC CCAAATTAGA ATTCTCAGCC TTCGGAGTAT ACAGCAAGCC AAGTGGTGTC 
CACTCCACAG AGGACACCAA AGCCACAGCC TTCCGGAACA CCACAGAGTC TCCAAGGCAT 
GTTTCAGAAT CCTCCAGAAG ATCTCTGAGC TCAAAACAAG AGTGGTCCAA GCCTCCTGGG 
AAGCCTGCCA ACGGGGGTGT TGTGAAATTA GAGAATTTCC ACCTGCGTCC ACTGCGATTC 
AAGGTCCCAG ATGTCCCCCA GGAAGCTGAG ACCCCCCAAA CCTGGGGCTG GGAAGTGGCC 
GGAGGCCCTG TGTTGAGACT ACAAAAGTCA CAGTCATCTG ACCTGCTGGA GAGGGAGGTG 
GAGAGCGTCC TGCGGAGGGA GCGAGAGGTA GCTGAAGAGC GGAGGAATGC CTTCTTCCCA 
GAGGTATTCT CCCCAGTGCT GGACCAGGAA GAGAGCCATG AGCAAGACTC CCGCAGTTCC 
TCTCGGGCAT CTGGCATCAC CGGCAGCTAT TCCGTGTCAG AATCACCATT ATTCACGCCC 
ATTCACCTGC ATTCCGGCCT GGTGTGGAAG GTGGAGGCCC CAGAGGACAG TTCTCCGCCA 
GGGCAGAAGA CTAGAAAGGA GTCGTGGTAT GCAGGTATCA ACCCCTCAGA CGGTGTCAAC 
TCGGAGGAGC TGGGAGTTCT CCATCTCGAT CGGAAGGCAG CTGAACAGGT CTTTCTGGTT 
TCCGCCTATG TGTTCCAAGC AGTGGAAGAG CACCCTACAG CGATATGTGA TAACCCTCCT 
CTCTTAACTT CTTGGCTCCC ACCTTATGAA TGTCAAAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Misp ( XM_017595044.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017450533.1 Rattus norvegicus mitotic spindle positioning (Misp), transcript variant X2, mRNA.

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CloneID	ORa62122
Clone ID Related Accession (Same CDS sequence)	XM_017595043.1 , XM_017595044.1
Accession Version	XM_017595044.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2022bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	mitotic interactor and substrate of PLK1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCGAG TGACCAGATA CCCCATCTTC AGCAAGCCCC ACTCAGCACG TGTCACCGGC 
TTGGCCTTGG ATGAAGACAC CAGCTACACC GTTGAGCTAG TGGGCGTAGG GCCTGAGGCT 
GGTTGGAGCC AGGGTGATCT GCAAACCTGG TCCGCTGAGG ACCAGACCAA GCCAGATGTG 
AAGAGAGTAA GCGTGTCTTC CACCAGACGA GCCTTCCTGG GGCAGCAGTC CCCGAGGTCA 
CTGTACTGGG AAGATGAGGA CGAGGAGACA AAAGCTTACC GCGTGGATGC CGGCAGCAAT 
GCCTTCTGCA GGCAGCCGAG GGACCTGGAG GCCGAGCGCT GGGCAGTCAT CCAGAGCCAG 
GCGGTCAGGA AGGGTGGCAC AGTGGCCACA CTCCAGGCTT CATCAGACCA TAGGGACCCC 
AGGATCGCCA GCCATCCCCG GTCTTCATTC ACAGAGGAGA TCTTGGTGGA CACGGAGCAA 
ATCGATTTCC TGGCTGCTCG GCAGCAGTTC CTGAGTTTAG AGAAGGCAAA CACAAATCCG 
GTCACGTGGA GCCCCACAGC CAGGGAGACC TTTGCACGTA CCCCACCTGG CGTCGACCAG 
ACCCCCAAGG CCTCCACTGG GCCCCATGTA GCCAATGGCT ACACCACTTC AGTCACGTCA 
CCAATGAAGG AAATGACCTT GGAGAAGACT TTCCATTTCC ACACTTCCCC AGCCGGGTCC 
ATCCGTACAA CGGATGACCC TGGCCACCAG ACCCGAGCAG AGTCCCCTGA GACCCCCAAG 
GAGACGCCCA TCGAGAGAGA GATCAGACTG GCCCAGGAGC GAGAGGCGGA ACTGCGGGAG 
CAGAGAGGGC TTGGGCGGGC TGCCGGGCAT CAAGAGCTCA TACAGATCCC CAGCAGGCCC 
CTGCTCAACA AGATGAGCCT GACAGAGACA CCCCCACGAA GAGACAGGGG CCGCCCATCC 
CTCTACGTGC AGAGGGACAT GGTGCAAGAA ACGCAACGAG AAGAAGATCA TCGCCGAGAG 
GGTCTGCAGG TGGGCAGGGC GTCCACACCT GACTGGACTT CCCAGGACCC TCAGCCTGGG 
TTCCAGAGAA GCCTGAGCTC CGACTGCATC CTCAGCCCAG ATGCCCGAGC TGCAGACCCA 
GCTCCAGAGG CAAGGAAAGT CAACCGGATC CCACCAGATG CCTACCAGCC ATACCTGGGC 
CCTGGGACCC CCAAATTAGA ATTCTCAGCC TTCGGAGTAT ACAGCAAGCC AAGTGGTGTC 
CACTCCACAG AGGACACCAA AGCCACAGCC TTCCGGAACA CCACAGAGTC TCCAAGGCAT 
GTTTCAGAAT CCTCCAGAAG ATCTCTGAGC TCAAAACAAG AGTGGTCCAA GCCTCCTGGG 
AAGCCTGCCA ACGGGGGTGT TGTGAAATTA GAGAATTTCC ACCTGCGTCC ACTGCGATTC 
AAGGTCCCAG ATGTCCCCCA GGAAGCTGAG ACCCCCCAAA CCTGGGGCTG GGAAGTGGCC 
GGAGGCCCTG TGTTGAGACT ACAAAAGTCA CAGTCATCTG ACCTGCTGGA GAGGGAGGTG 
GAGAGCGTCC TGCGGAGGGA GCGAGAGGTA GCTGAAGAGC GGAGGAATGC CTTCTTCCCA 
GAGGTATTCT CCCCAGTGCT GGACCAGGAA GAGAGCCATG AGCAAGACTC CCGCAGTTCC 
TCTCGGGCAT CTGGCATCAC CGGCAGCTAT TCCGTGTCAG AATCACCATT ATTCACGCCC 
ATTCACCTGC ATTCCGGCCT GGTGTGGAAG GTGGAGGCCC CAGAGGACAG TTCTCCGCCA 
GGGCAGAAGA CTAGAAAGGA GTCGTGGTAT GCAGGTATCA ACCCCTCAGA CGGTGTCAAC 
TCGGAGGAGC TGGGAGTTCT CCATCTCGAT CGGAAGGCAG CTGAACAGGT CTTTCTGGTT 
TCCGCCTATG TGTTCCAAGC AGTGGAAGAG CACCCTACAG CGATATGTGA TAACCCTCCT 
CTCTTAACTT CTTGGCTCCC ACCTTATGAA TGTCAAAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017450533.1
CDS	1743..3764
Translation	MDRVTRYPIFSKPHSARVTGLALDEDTSYTVELVGVGPEAGWSQGDLQTWSAEDQTKPDVKRVSVSSTRRAFLGQQSPRSLYWEDEDEETKAYRVDAGSNAFCRQPRDLEAERWAVIQSQAVRKGGTVATLQASSDHRDPRIASHPRSSFTEEILVDTEQIDFLAARQQFLSLEKANTNPVTWSPTARETFARTPPGVDQTPKASTGPHVANGYTTSVTSPMKEMTLEKTFHFHTSPAGSIRTTDDPGHQTRAESPETPKETPIEREIRLAQEREAELREQRGLGRAAGHQELIQIPSRPLLNKMSLTETPPRRDRGRPSLYVQRDMVQETQREEDHRREGLQVGRASTPDWTSQDPQPGFQRSLSSDCILSPDARAADPAPEARKVNRIPPDAYQPYLGPGTPKLEFSAFGVYSKPSGVHSTEDTKATAFRNTTESPRHVSESSRRSLSSKQEWSKPPGKPANGGVVKLENFHLRPLRFKVPDVPQEAETPQTWGWEVAGGPVLRLQKSQSSDLLEREVESVLRREREVAEERRNAFFPEVFSPVLDQEESHEQDSRSSSRASGITGSYSVSESPLFTPIHLHSGLVWKVEAPEDSSPPGQKTRKESWYAGINPSDGVNSEELGVLHLDRKAAEQVFLVSAYVFQAVEEHPTAICDNPPLLTSWLPPYECQS

Target ORF information:

RefSeq Version	XM_017595044.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mitotic spindle positioning (Misp), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017595044.1

ORF Insert Sequence:

ATGGACCGAG TGACCAGATA CCCCATCTTC AGCAAGCCCC ACTCAGCACG TGTCACCGGC 
TTGGCCTTGG ATGAAGACAC CAGCTACACC GTTGAGCTAG TGGGCGTAGG GCCTGAGGCT 
GGTTGGAGCC AGGGTGATCT GCAAACCTGG TCCGCTGAGG ACCAGACCAA GCCAGATGTG 
AAGAGAGTAA GCGTGTCTTC CACCAGACGA GCCTTCCTGG GGCAGCAGTC CCCGAGGTCA 
CTGTACTGGG AAGATGAGGA CGAGGAGACA AAAGCTTACC GCGTGGATGC CGGCAGCAAT 
GCCTTCTGCA GGCAGCCGAG GGACCTGGAG GCCGAGCGCT GGGCAGTCAT CCAGAGCCAG 
GCGGTCAGGA AGGGTGGCAC AGTGGCCACA CTCCAGGCTT CATCAGACCA TAGGGACCCC 
AGGATCGCCA GCCATCCCCG GTCTTCATTC ACAGAGGAGA TCTTGGTGGA CACGGAGCAA 
ATCGATTTCC TGGCTGCTCG GCAGCAGTTC CTGAGTTTAG AGAAGGCAAA CACAAATCCG 
GTCACGTGGA GCCCCACAGC CAGGGAGACC TTTGCACGTA CCCCACCTGG CGTCGACCAG 
ACCCCCAAGG CCTCCACTGG GCCCCATGTA GCCAATGGCT ACACCACTTC AGTCACGTCA 
CCAATGAAGG AAATGACCTT GGAGAAGACT TTCCATTTCC ACACTTCCCC AGCCGGGTCC 
ATCCGTACAA CGGATGACCC TGGCCACCAG ACCCGAGCAG AGTCCCCTGA GACCCCCAAG 
GAGACGCCCA TCGAGAGAGA GATCAGACTG GCCCAGGAGC GAGAGGCGGA ACTGCGGGAG 
CAGAGAGGGC TTGGGCGGGC TGCCGGGCAT CAAGAGCTCA TACAGATCCC CAGCAGGCCC 
CTGCTCAACA AGATGAGCCT GACAGAGACA CCCCCACGAA GAGACAGGGG CCGCCCATCC 
CTCTACGTGC AGAGGGACAT GGTGCAAGAA ACGCAACGAG AAGAAGATCA TCGCCGAGAG 
GGTCTGCAGG TGGGCAGGGC GTCCACACCT GACTGGACTT CCCAGGACCC TCAGCCTGGG 
TTCCAGAGAA GCCTGAGCTC CGACTGCATC CTCAGCCCAG ATGCCCGAGC TGCAGACCCA 
GCTCCAGAGG CAAGGAAAGT CAACCGGATC CCACCAGATG CCTACCAGCC ATACCTGGGC 
CCTGGGACCC CCAAATTAGA ATTCTCAGCC TTCGGAGTAT ACAGCAAGCC AAGTGGTGTC 
CACTCCACAG AGGACACCAA AGCCACAGCC TTCCGGAACA CCACAGAGTC TCCAAGGCAT 
GTTTCAGAAT CCTCCAGAAG ATCTCTGAGC TCAAAACAAG AGTGGTCCAA GCCTCCTGGG 
AAGCCTGCCA ACGGGGGTGT TGTGAAATTA GAGAATTTCC ACCTGCGTCC ACTGCGATTC 
AAGGTCCCAG ATGTCCCCCA GGAAGCTGAG ACCCCCCAAA CCTGGGGCTG GGAAGTGGCC 
GGAGGCCCTG TGTTGAGACT ACAAAAGTCA CAGTCATCTG ACCTGCTGGA GAGGGAGGTG 
GAGAGCGTCC TGCGGAGGGA GCGAGAGGTA GCTGAAGAGC GGAGGAATGC CTTCTTCCCA 
GAGGTATTCT CCCCAGTGCT GGACCAGGAA GAGAGCCATG AGCAAGACTC CCGCAGTTCC 
TCTCGGGCAT CTGGCATCAC CGGCAGCTAT TCCGTGTCAG AATCACCATT ATTCACGCCC 
ATTCACCTGC ATTCCGGCCT GGTGTGGAAG GTGGAGGCCC CAGAGGACAG TTCTCCGCCA 
GGGCAGAAGA CTAGAAAGGA GTCGTGGTAT GCAGGTATCA ACCCCTCAGA CGGTGTCAAC 
TCGGAGGAGC TGGGAGTTCT CCATCTCGAT CGGAAGGCAG CTGAACAGGT CTTTCTGGTT 
TCCGCCTATG TGTTCCAAGC AGTGGAAGAG CACCCTACAG CGATATGTGA TAACCCTCCT 
CTCTTAACTT CTTGGCTCCC ACCTTATGAA TGTCAAAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The novel actin/focal adhesion-associated protein MISP is involved in mitotic spindle positioning in human cells.
Cell cycle (Georgetown, Tex.)12(9)1457-71(2013 May)
Maier B,Kirsch M,Anderhub S,Zentgraf H,Krämer A

MISP is a novel Plk1 substrate required for proper spindle orientation and mitotic progression.
The Journal of cell biology200(6)773-87(2013 Mar)
Zhu M,Settele F,Kotak S,Sanchez-Pulido L,Ehret L,Ponting CP,Gönczy P,Hoffmann I

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Misp cDNA ORF clone, Rattus norvegicus(Norway rat)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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