Ubr1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ubr1
Entrez Gene ID	499877
Full Name	ubiquitin protein ligase E3 component n-recognin 1
Synonyms	RGD1562326
General protein information	Preferred Names ubiquitin protein ligase E3 component n-recognin 1 Names E3 ubiquitin-protein ligase UBR1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q35

mRNA and Protein(s)

mRNA	Protein	Name
NM_001178072.1	NP_001171543.1	E3 ubiquitin-protein ligase UBR1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	UBR1	XP_510341.3
Macaca mulatta (Rhesus monkey)	UBR1	XP_001105721.2
Canis lupus familiaris (dog)	UBR1	XP_544645.2
Bos taurus (cattle)	UBR1	NP_001192826.1
Danio rerio (zebrafish)	ubr1	XP_002665264.3
Xenopus tropicalis (tropical clawed frog)	ubr1	XP_002941132.2
Rattus norvegicus (Norway rat)	Ubr1	NP_001171543.1
Schizosaccharomyces pombe (fission yeast)	ubr1	NP_596158.2
Homo sapiens (human)	UBR1	NP_777576.1
Mus musculus (house mouse)	Ubr1	NP_033487.2
Gallus gallus (chicken)	UBR1	XP_421165.3
Magnaporthe oryzae (rice blast fungus)	MGG_13171	XP_003710667.1
Neurospora crassa	NCU03234	XP_965334.2

Gene Ontology
Bibliography

Related articles in PubMed

Role of N-end rule ubiquitin ligases UBR1 and UBR2 in regulating the leucine-mTOR signaling pathway.
Kume K, Iizumi Y, Shimada M, Ito Y, Kishi T, Yamaguchi Y, Handa H
Genes to cells : devoted to molecular & cellular mechanisms15(4)339-49(2010 Apr)

Female lethality and apoptosis of spermatocytes in mice lacking the UBR2 ubiquitin ligase of the N-end rule pathway.
Kwon YT, Xia Z, An JY, Tasaki T, Davydov IV, Seo JW, Sheng J, Xie Y, Varshavsky A
Molecular and cellular biology23(22)8255-71(2003 Nov)

Construction and analysis of mouse strains lacking the ubiquitin ligase UBR1 (E3alpha) of the N-end rule pathway.
Kwon YT, Xia Z, Davydov IV, Lecker SH, Varshavsky A
Molecular and cellular biology21(23)8007-21(2001 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ubr1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ubr1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa06733	NM_001178072.1 Latest version!	Rattus norvegicus ubiquitin protein ligase E3 component n-recognin 1 (Ubr1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ubr1 ( NM_001178072.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001171543.1 Rattus norvegicus ubiquitin protein ligase E3 component n-recognin 1 (Ubr1), mRNA.

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CloneID	ORa06733
Clone ID Related Accession (Same CDS sequence)	NM_001178072.1
Accession Version	NM_001178072.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5274bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1506787200000
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase UBR1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB549211.1. On or before May 14, 2010 this sequence version replaced XR_085791.2, XR_086198.1. ##Evidence-Data-START## Transcript exon combination :: AB549211.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCGGACG AAGAGATGGA CGGCGCCGAG AGGATGGACG TCAGCCCGGA GCCTCCCCTG 
GCCCCTCAGC CGCCCGCATC CTGGTGGGAT CAGCAAGGTG ATTTTTATAC TGCTTTCTTA 
CATCATTTGG CAAAATTAGT GCCAGAAATT TATTTTGCTG AGATGGACCC AGATTTGGAA 
AAGCAAGAAG AGAATGTACA AATGTCAATA TTCACTCCTT TGGAATGGTA CTTATTTGGA 
GAGGATCCAG ACGTTTGCTT AGAGAGATTA AAGCACAGTG CAGCATTTCA GTTGTGCGGG 
AAGGTTTTCA AAAGTGGAGA AACAACATAT TCCTGTAGGG ATTGTGCAAT TGATCCAACG 
TGTGTGCTCT GTATGGACTG CTTCCAGAGT AGTGTTCATA AAAACCATCG TTACAAGATG 
CATACTTCTA CTGGAGGAGG CTTCTGTGAC TGTGGAGACA CAGAAGCGTG GAAAACTGGT 
CCCTTTTGTA TGGATCACGA ACCTGGAAGA GCAGGTACTA CAAAAGAGAC CTTGCATTGC 
CCACTGAATG AAGAGGTAAT TGCTCAAGCC AGGAGAATAT TCCCTTCAGT GATAAAGTAC 
ATTGTAGAAA TGACTATATG GGAAGAGGAA AAGGAACTGC CTCCTGAACT GCAGAGAAGG 
GAGAAAAATG AAAGATATTA CTGTGTCCTT TTCAACGATG AGCACCATTC ATATGATCAT 
GTGATATACA GTCTGCAAAG AGCTCTTGAC TGTGAGCTTG CAGAGGCACA GTTGCACACA 
ACTGCCATAG ACAAAGAGGG TCGCCGGGCT GTCAAAGCAG GTGTGTATTC CGCTTGCCAG 
GAAGCAAAGG AGGATATAAA GAGTCACTCA GAGAACGTCT CTCAGCACCC CCTCCATGTG 
GAAGTGCTGC ATTCCGTGGT TATGGCCCAC CAGAAATTTG CTCTGCGCCT TGGATCCTGG 
ATGAACAAAA TTATGAGCTA TTCAAGTGAC TTTAGGCAGA TCTTCTGCCA AGCCTGCCTC 
ATAGAGGAGC CTGGCTCTGA ACATCCCTGT CTTATAAGCA GACTAATGCT TTGGGATGCA 
AAACTTTATA AAGGTGCCCG TAAGATCCTT CACGAATTGA TCTTTAGTAG TTTTTTTATG 
GAGATGGAAT ACAAAAAACT CTTTGCTATG GAATTTGTGA AGTATTATAA ACAACTACAG 
AAAGAGTACA TCAGTGACGA CCACGAGAGA AGCATCTCTA TAACCGCACT GTCCGTTCAG 
GTGTTCACTG TCCCCACCTT GGCCCGACAT CTTATTGAAG AGCAGAATGT TATTTCTGTC 
ATTACTGAAA CTCTGCTGGA AGTTCTACCT GAGTACTTGG ACAGGAACAA TAAGTTCAAC 
TTCCAGGGCT ATAGCCAGGA CAAGCTGGGA AGAGTGTACG CGGTGATCTG TGACCTGAAG 
TACATCCTGA TTAGCAAGCC TCTCGTATGG ACAGAAAGAC TAAGAGCACA GTTCCTGGAA 
GGGTTCCGGT CTTTTCTGAA GATTCTCACC TGTATGCAGG GAATGGAAGA AATCAGAAGA 
CAAGTTGGAC AGCACATTGA AGTAGACCCT GACTGGGAGG CTGCCATCGC TATACAGATG 
CAACTAAAGA ATATTTTGCT CATGTTCCAA GAGTGGTGTG CTTGTGATGA AGATCTCTTA 
CTAGTGGCTT ATAAAGAATG TCACAAAGCT GTAATGAGGT GCAGCACAAA TTTCATGTCT 
AGTACCAAGA CAGTAGTACA ACTGTGTGGT CATAGTTTGG AAACTAAATC CTACAAAGTG 
TCTGAGGACC TTGTCAGCAT ACATCTACCA CTCTCTAGAA CTCTCGCTGG TCTTCATGTA 
CGTTTAAGCA GACTAGGTGC TGTTTCAAGA CTGCATGAAT TTGTGCCTTT TGATGGCTTT 
CAAGTAGAGG TCCTAGTAGA GTACCCTTTG CGCTGCCTGG TGCTGGTTGC TCAGGTTGTT 
GCCGAGATGT GGCGAAGAAA CGGGCTCTCC CTCATTAGCC AGGTTTTCTA TTATCAAGAT 
GTTAAATGCA GGGAAGAAAT GTATGATAAA GATATCATCA TGCTTCAGAT TGGAGCGTCT 
ATAATGGATC CCAACAAGTT CTTGTTACTG GTACTTCAGA GGTATGAACT TGCTGATGCT 
TTTAACAAGA CCATATCCAC AAAAGACCAG GATTTGATTA AACAGTATAA CACATTAATA 
GAAGAAATGC TTCAGGTCCT CATCTATATT GTGGGAGAAC GTTATGTACC CGGAGTGGGA 
AACGTGACCA AAGAGGAGGT TATAATGAGA GAGATCACTC ACTTACTTTG CATTGAGCCC 
ATGCCACACA GTGCCATTGC CAGAAACCTA CCGGAGAATG AAAATAACGA AACTGGCTTA 
GAGAATGTCA TAAACAAAGT GGCCACATTT AAGAAACCGG GTGTGTCGGG CCATGGTGTT 
TATGAATTGA AAGACGAATC ACTGAAAGAC TTCAATATGT ACTTTTACCA TTATTCTAAA 
ACACAGCATA GCAAGGCTGA ACATATGCAG AAGAAGAGGA GAAAACAAGA AAATAAAGAT 
GAAGCCTTGC CACCGCCACC TCCTCCTGAG TTCTGTCCCG CTTTCAGCAA AGTAACTGAC 
CTTCTCAACT GTGATATTAT GATGCACATT CTCAGGACCA TCTTCGAGCG GGCAGTGGAC 
CCCGAGTCTA ATCTGTGGAC AGAAGGGATG CTCCAAATGG CCTTCCATAT ATTGGCGCTG 
GGCTTGCTGG AGGAGAAGCA GCAGCTTCAG AAAGCTCCTG AAGAAGAAGT GGCTTTCGAC 
TTTTACCATA AGGCTTCAAG ATTGGGAAGT TCAGCAATGA ATGCTCAGAA TATACAAATG 
CTTTTGGAAA AACTCAAAGG AATCCCCCAA TTAGAAGGCC AGAAGGACAT GATAACATGG 
ATTCTCCAGA TGTTTGACAC AGTGAAGCGA TTAAGAGAAA AATCTTGTTT AGTTGTGGCA 
AGCACTTCAG GATTGGAGTC CATTAAAAGT GAGGAGATTA CTCATGATAA AGAAAAAGCA 
GAACGGAAGA GAAAAGCTGA AGCTGCTAGG CTACATCGCC AGAAGATCAT GGCCCAGATG 
TCTGCCTTAC AGAAAAACTT CATTGAAACT CACAAACTCA TGTATGACAA TACATCAGAA 
ATAACAGGGA AGGAAGACTC CATTATGGAA GAAGAAAGCC CCTCAGCAGT CAGTGAGTGC 
TCTCGAATTG CTCTGGGCCC TAAACGGGGC CCAGCTCTTA CAGAAAAGGA GGTGCTGACG 
TGCATCCTCT GCCAAGAAGA ACAAGAGGTG AAACTAGAAA ACAACGCCAT GGTGTTGTCC 
GCCTGTGTGC AGAAATCCAC CGCCCTAACC CAGCACAGGG GGAAGCCTGC GGGCCTCTTA 
GGGGAATCAC TGGACCCTCT TTTCATGGAT CCAGACCTGG CACATGGCAC TTACGCAGGA 
AGCTGTGGTC ATGTAATGCA TGCAGTGTGC TGGCAGAAGT ATTTTGAAGC AGTGCAGCTG 
AGCTCTCAGC AGCGCATTCA CGTCGACCTG TTTGACTTGG AGAGTGGAGA GTACCTGTGC 
CCTCTCTGCA AGTCTCTCTG CAATACCGTC ATCCCCATCA TTCCTTTGCA GCCACAGAAG 
ATCAACAGTG AGAATGCAGA GGCTCTTTCT CAACTTTTGA CCTTGGCACG GTGGATACAG 
ACTGTCCTTG CAAGAATATC AGGTTATAAT ATGAAACACG CTAAAGGAGA AGCTCCAGCA 
GTTCCTGTCC TGTTTAATCA AGGAATGGGG GATTCTACTT TTGAGTTTCA TTCCATCCTG 
AGTTTTGGAG TTCAGTCTTC GGTGAAATAC TCAAATAGTA TCAAGGAAAT GGTCATTCTC 
TTTGCCACAA CAATTTACAG AATCGGCCTG AAAGTGCCTC CTGATGAATT AGACCCACGA 
GTACCCATGA TGACCTGGAG CACGTGTGCT TTCACCATCC AGGCAATTGA AAACCTTTTG 
GGAGATGAAG GAAAACCTCT ATTTGGAGCA CTTCAAAATA GACAGCATAA TGGTCTGAAA 
GCGTTAATGC AATTTGCAGT TGCACAGAGG ACTACCTGTC CTCAGGTCCT GATACAGAAA 
CATCTGGTTC GACTCCTATC AGTTGTTCTT CCTAACCTAC AATCAGAAAA TACACCATGC 
CTTCTATCCG TTGATCTATT TCATGTTCTG GTGGGCGCTG TCTTAGCGTT CCCGTCCTTG 
TACTGGGATG ACACGGTGGA TCTGCAGCCT TCACCGCTCA GCTCTTCATA CAGCCACCTT 
TATCTCTTCC ATCTGGTCAC CATGGCCCAT ATGCTTCAGA TACTCCTCAC GGTAGATGCA 
GATCAGTCTC CGGGACTCCC ACTTAATGAG GGTGGAGAGG ACAGTGAAGA GGCTCGCTGT 
GCGTCTGCTC TCTTCGTGGA AGTCTCACAG CACACAGACG GCCTCATTGG GTGTGGTGCT 
CCTGGCTGGT ACCTGTGGCT CTCCCTGAGG AATGGCATCA CCCCTTACCT CCGCTGTGCT 
GCACTGCTTT TCCACTATTT ACTCGGAGTA ACTCCGCCTG AAGAACTGTT TGCCAACTCT 
GCTGAAGGAG AATTCAGTGC ACTCTGTAGC TATCTCTCTT TACCCACAAA TTTGTTCCTG 
CTTTTCCAGG AATATTGGGA TACCATAAGG CCCTTACTTC AGAGGTGGTG TGGAGATCCT 
GCCTTACTCA ACTCTTTGAA GCAGAAAAGT GCTCTGGTCA GGTACCCTAG AAAAAGAAAT 
AGTTTGATAG AGCTTCCTGA TGACTACAGC TGTCTTCTAA ATCAAGCTTC TCACTTTAGG 
TGTCCACGGT CTTCAGATGA TGAACGAAAG CACCCTGTCC TCTGTCTTTT CTGTGGGGCC 
ATCCTGTGTT CTCAGAACAT CTGTTGCCAA GAAATCGTGA ATGGGGAGGA GGTTGGAGCG 
TGCGTTTTTC ATGCACTTCA GTGTGGTGCT GGAGTCTGCA TTTTCCTAAA AATCAGAGAA 
TGCAGAGTGG TCCTGGTAGA AGGAAAAGCC AGAGGCTGTG CCTACCCAGC CCCTTACTTG 
GATGAATATG GAGAAACAGA CCCAGGGCTA AAGAGGGGAA ACCCACTTCA TTTATCTCGG 
GAGCGGTATC GGAAGCTGCA TTTGGTCTGG CAACAGCACT GCATTATAGA AGAGATTGCT 
CGGAGCCAGG AGACTAATCA GATGCTGTTT GGATTCAACT GGCAGTTACT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001171543.1
CDS	1..5274
Translation	MADEEMDGAERMDVSPEPPLAPQPPASWWDQQGDFYTAFLHHLAKLVPEIYFAEMDPDLEKQEENVQMSIFTPLEWYLFGEDPDVCLERLKHSAAFQLCGKVFKSGETTYSCRDCAIDPTCVLCMDCFQSSVHKNHRYKMHTSTGGGFCDCGDTEAWKTGPFCMDHEPGRAGTTKETLHCPLNEEVIAQARRIFPSVIKYIVEMTIWEEEKELPPELQRREKNERYYCVLFNDEHHSYDHVIYSLQRALDCELAEAQLHTTAIDKEGRRAVKAGVYSACQEAKEDIKSHSENVSQHPLHVEVLHSVVMAHQKFALRLGSWMNKIMSYSSDFRQIFCQACLIEEPGSEHPCLISRLMLWDAKLYKGARKILHELIFSSFFMEMEYKKLFAMEFVKYYKQLQKEYISDDHERSISITALSVQVFTVPTLARHLIEEQNVISVITETLLEVLPEYLDRNNKFNFQGYSQDKLGRVYAVICDLKYILISKPLVWTERLRAQFLEGFRSFLKILTCMQGMEEIRRQVGQHIEVDPDWEAAIAIQMQLKNILLMFQEWCACDEDLLLVAYKECHKAVMRCSTNFMSSTKTVVQLCGHSLETKSYKVSEDLVSIHLPLSRTLAGLHVRLSRLGAVSRLHEFVPFDGFQVEVLVEYPLRCLVLVAQVVAEMWRRNGLSLISQVFYYQDVKCREEMYDKDIIMLQIGASIMDPNKFLLLVLQRYELADAFNKTISTKDQDLIKQYNTLIEEMLQVLIYIVGERYVPGVGNVTKEEVIMREITHLLCIEPMPHSAIARNLPENENNETGLENVINKVATFKKPGVSGHGVYELKDESLKDFNMYFYHYSKTQHSKAEHMQKKRRKQENKDEALPPPPPPEFCPAFSKVTDLLNCDIMMHILRTIFERAVDPESNLWTEGMLQMAFHILALGLLEEKQQLQKAPEEEVAFDFYHKASRLGSSAMNAQNIQMLLEKLKGIPQLEGQKDMITWILQMFDTVKRLREKSCLVVASTSGLESIKSEEITHDKEKAERKRKAEAARLHRQKIMAQMSALQKNFIETHKLMYDNTSEITGKEDSIMEEESPSAVSECSRIALGPKRGPALTEKEVLTCILCQEEQEVKLENNAMVLSACVQKSTALTQHRGKPAGLLGESLDPLFMDPDLAHGTYAGSCGHVMHAVCWQKYFEAVQLSSQQRIHVDLFDLESGEYLCPLCKSLCNTVIPIIPLQPQKINSENAEALSQLLTLARWIQTVLARISGYNMKHAKGEAPAVPVLFNQGMGDSTFEFHSILSFGVQSSVKYSNSIKEMVILFATTIYRIGLKVPPDELDPRVPMMTWSTCAFTIQAIENLLGDEGKPLFGALQNRQHNGLKALMQFAVAQRTTCPQVLIQKHLVRLLSVVLPNLQSENTPCLLSVDLFHVLVGAVLAFPSLYWDDTVDLQPSPLSSSYSHLYLFHLVTMAHMLQILLTVDADQSPGLPLNEGGEDSEEARCASALFVEVSQHTDGLIGCGAPGWYLWLSLRNGITPYLRCAALLFHYLLGVTPPEELFANSAEGEFSALCSYLSLPTNLFLLFQEYWDTIRPLLQRWCGDPALLNSLKQKSALVRYPRKRNSLIELPDDYSCLLNQASHFRCPRSSDDERKHPVLCLFCGAILCSQNICCQEIVNGEEVGACVFHALQCGAGVCIFLKIRECRVVLVEGKARGCAYPAPYLDEYGETDPGLKRGNPLHLSRERYRKLHLVWQQHCIIEEIARSQETNQMLFGFNWQLL

Target ORF information:

RefSeq Version	NM_001178072.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ubiquitin protein ligase E3 component n-recognin 1 (Ubr1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001178072.1

ORF Insert Sequence:

ATGGCGGACG AAGAGATGGA CGGCGCCGAG AGGATGGACG TCAGCCCGGA GCCTCCCCTG 
GCCCCTCAGC CGCCCGCATC CTGGTGGGAT CAGCAAGGTG ATTTTTATAC TGCTTTCTTA 
CATCATTTGG CAAAATTAGT GCCAGAAATT TATTTTGCTG AGATGGACCC AGATTTGGAA 
AAGCAAGAAG AGAATGTACA AATGTCAATA TTCACTCCTT TGGAATGGTA CTTATTTGGA 
GAGGATCCAG ACGTTTGCTT AGAGAGATTA AAGCACAGTG CAGCATTTCA GTTGTGCGGG 
AAGGTTTTCA AAAGTGGAGA AACAACATAT TCCTGTAGGG ATTGTGCAAT TGATCCAACG 
TGTGTGCTCT GTATGGACTG CTTCCAGAGT AGTGTTCATA AAAACCATCG TTACAAGATG 
CATACTTCTA CTGGAGGAGG CTTCTGTGAC TGTGGAGACA CAGAAGCGTG GAAAACTGGT 
CCCTTTTGTA TGGATCACGA ACCTGGAAGA GCAGGTACTA CAAAAGAGAC CTTGCATTGC 
CCACTGAATG AAGAGGTAAT TGCTCAAGCC AGGAGAATAT TCCCTTCAGT GATAAAGTAC 
ATTGTAGAAA TGACTATATG GGAAGAGGAA AAGGAACTGC CTCCTGAACT GCAGAGAAGG 
GAGAAAAATG AAAGATATTA CTGTGTCCTT TTCAACGATG AGCACCATTC ATATGATCAT 
GTGATATACA GTCTGCAAAG AGCTCTTGAC TGTGAGCTTG CAGAGGCACA GTTGCACACA 
ACTGCCATAG ACAAAGAGGG TCGCCGGGCT GTCAAAGCAG GTGTGTATTC CGCTTGCCAG 
GAAGCAAAGG AGGATATAAA GAGTCACTCA GAGAACGTCT CTCAGCACCC CCTCCATGTG 
GAAGTGCTGC ATTCCGTGGT TATGGCCCAC CAGAAATTTG CTCTGCGCCT TGGATCCTGG 
ATGAACAAAA TTATGAGCTA TTCAAGTGAC TTTAGGCAGA TCTTCTGCCA AGCCTGCCTC 
ATAGAGGAGC CTGGCTCTGA ACATCCCTGT CTTATAAGCA GACTAATGCT TTGGGATGCA 
AAACTTTATA AAGGTGCCCG TAAGATCCTT CACGAATTGA TCTTTAGTAG TTTTTTTATG 
GAGATGGAAT ACAAAAAACT CTTTGCTATG GAATTTGTGA AGTATTATAA ACAACTACAG 
AAAGAGTACA TCAGTGACGA CCACGAGAGA AGCATCTCTA TAACCGCACT GTCCGTTCAG 
GTGTTCACTG TCCCCACCTT GGCCCGACAT CTTATTGAAG AGCAGAATGT TATTTCTGTC 
ATTACTGAAA CTCTGCTGGA AGTTCTACCT GAGTACTTGG ACAGGAACAA TAAGTTCAAC 
TTCCAGGGCT ATAGCCAGGA CAAGCTGGGA AGAGTGTACG CGGTGATCTG TGACCTGAAG 
TACATCCTGA TTAGCAAGCC TCTCGTATGG ACAGAAAGAC TAAGAGCACA GTTCCTGGAA 
GGGTTCCGGT CTTTTCTGAA GATTCTCACC TGTATGCAGG GAATGGAAGA AATCAGAAGA 
CAAGTTGGAC AGCACATTGA AGTAGACCCT GACTGGGAGG CTGCCATCGC TATACAGATG 
CAACTAAAGA ATATTTTGCT CATGTTCCAA GAGTGGTGTG CTTGTGATGA AGATCTCTTA 
CTAGTGGCTT ATAAAGAATG TCACAAAGCT GTAATGAGGT GCAGCACAAA TTTCATGTCT 
AGTACCAAGA CAGTAGTACA ACTGTGTGGT CATAGTTTGG AAACTAAATC CTACAAAGTG 
TCTGAGGACC TTGTCAGCAT ACATCTACCA CTCTCTAGAA CTCTCGCTGG TCTTCATGTA 
CGTTTAAGCA GACTAGGTGC TGTTTCAAGA CTGCATGAAT TTGTGCCTTT TGATGGCTTT 
CAAGTAGAGG TCCTAGTAGA GTACCCTTTG CGCTGCCTGG TGCTGGTTGC TCAGGTTGTT 
GCCGAGATGT GGCGAAGAAA CGGGCTCTCC CTCATTAGCC AGGTTTTCTA TTATCAAGAT 
GTTAAATGCA GGGAAGAAAT GTATGATAAA GATATCATCA TGCTTCAGAT TGGAGCGTCT 
ATAATGGATC CCAACAAGTT CTTGTTACTG GTACTTCAGA GGTATGAACT TGCTGATGCT 
TTTAACAAGA CCATATCCAC AAAAGACCAG GATTTGATTA AACAGTATAA CACATTAATA 
GAAGAAATGC TTCAGGTCCT CATCTATATT GTGGGAGAAC GTTATGTACC CGGAGTGGGA 
AACGTGACCA AAGAGGAGGT TATAATGAGA GAGATCACTC ACTTACTTTG CATTGAGCCC 
ATGCCACACA GTGCCATTGC CAGAAACCTA CCGGAGAATG AAAATAACGA AACTGGCTTA 
GAGAATGTCA TAAACAAAGT GGCCACATTT AAGAAACCGG GTGTGTCGGG CCATGGTGTT 
TATGAATTGA AAGACGAATC ACTGAAAGAC TTCAATATGT ACTTTTACCA TTATTCTAAA 
ACACAGCATA GCAAGGCTGA ACATATGCAG AAGAAGAGGA GAAAACAAGA AAATAAAGAT 
GAAGCCTTGC CACCGCCACC TCCTCCTGAG TTCTGTCCCG CTTTCAGCAA AGTAACTGAC 
CTTCTCAACT GTGATATTAT GATGCACATT CTCAGGACCA TCTTCGAGCG GGCAGTGGAC 
CCCGAGTCTA ATCTGTGGAC AGAAGGGATG CTCCAAATGG CCTTCCATAT ATTGGCGCTG 
GGCTTGCTGG AGGAGAAGCA GCAGCTTCAG AAAGCTCCTG AAGAAGAAGT GGCTTTCGAC 
TTTTACCATA AGGCTTCAAG ATTGGGAAGT TCAGCAATGA ATGCTCAGAA TATACAAATG 
CTTTTGGAAA AACTCAAAGG AATCCCCCAA TTAGAAGGCC AGAAGGACAT GATAACATGG 
ATTCTCCAGA TGTTTGACAC AGTGAAGCGA TTAAGAGAAA AATCTTGTTT AGTTGTGGCA 
AGCACTTCAG GATTGGAGTC CATTAAAAGT GAGGAGATTA CTCATGATAA AGAAAAAGCA 
GAACGGAAGA GAAAAGCTGA AGCTGCTAGG CTACATCGCC AGAAGATCAT GGCCCAGATG 
TCTGCCTTAC AGAAAAACTT CATTGAAACT CACAAACTCA TGTATGACAA TACATCAGAA 
ATAACAGGGA AGGAAGACTC CATTATGGAA GAAGAAAGCC CCTCAGCAGT CAGTGAGTGC 
TCTCGAATTG CTCTGGGCCC TAAACGGGGC CCAGCTCTTA CAGAAAAGGA GGTGCTGACG 
TGCATCCTCT GCCAAGAAGA ACAAGAGGTG AAACTAGAAA ACAACGCCAT GGTGTTGTCC 
GCCTGTGTGC AGAAATCCAC CGCCCTAACC CAGCACAGGG GGAAGCCTGC GGGCCTCTTA 
GGGGAATCAC TGGACCCTCT TTTCATGGAT CCAGACCTGG CACATGGCAC TTACGCAGGA 
AGCTGTGGTC ATGTAATGCA TGCAGTGTGC TGGCAGAAGT ATTTTGAAGC AGTGCAGCTG 
AGCTCTCAGC AGCGCATTCA CGTCGACCTG TTTGACTTGG AGAGTGGAGA GTACCTGTGC 
CCTCTCTGCA AGTCTCTCTG CAATACCGTC ATCCCCATCA TTCCTTTGCA GCCACAGAAG 
ATCAACAGTG AGAATGCAGA GGCTCTTTCT CAACTTTTGA CCTTGGCACG GTGGATACAG 
ACTGTCCTTG CAAGAATATC AGGTTATAAT ATGAAACACG CTAAAGGAGA AGCTCCAGCA 
GTTCCTGTCC TGTTTAATCA AGGAATGGGG GATTCTACTT TTGAGTTTCA TTCCATCCTG 
AGTTTTGGAG TTCAGTCTTC GGTGAAATAC TCAAATAGTA TCAAGGAAAT GGTCATTCTC 
TTTGCCACAA CAATTTACAG AATCGGCCTG AAAGTGCCTC CTGATGAATT AGACCCACGA 
GTACCCATGA TGACCTGGAG CACGTGTGCT TTCACCATCC AGGCAATTGA AAACCTTTTG 
GGAGATGAAG GAAAACCTCT ATTTGGAGCA CTTCAAAATA GACAGCATAA TGGTCTGAAA 
GCGTTAATGC AATTTGCAGT TGCACAGAGG ACTACCTGTC CTCAGGTCCT GATACAGAAA 
CATCTGGTTC GACTCCTATC AGTTGTTCTT CCTAACCTAC AATCAGAAAA TACACCATGC 
CTTCTATCCG TTGATCTATT TCATGTTCTG GTGGGCGCTG TCTTAGCGTT CCCGTCCTTG 
TACTGGGATG ACACGGTGGA TCTGCAGCCT TCACCGCTCA GCTCTTCATA CAGCCACCTT 
TATCTCTTCC ATCTGGTCAC CATGGCCCAT ATGCTTCAGA TACTCCTCAC GGTAGATGCA 
GATCAGTCTC CGGGACTCCC ACTTAATGAG GGTGGAGAGG ACAGTGAAGA GGCTCGCTGT 
GCGTCTGCTC TCTTCGTGGA AGTCTCACAG CACACAGACG GCCTCATTGG GTGTGGTGCT 
CCTGGCTGGT ACCTGTGGCT CTCCCTGAGG AATGGCATCA CCCCTTACCT CCGCTGTGCT 
GCACTGCTTT TCCACTATTT ACTCGGAGTA ACTCCGCCTG AAGAACTGTT TGCCAACTCT 
GCTGAAGGAG AATTCAGTGC ACTCTGTAGC TATCTCTCTT TACCCACAAA TTTGTTCCTG 
CTTTTCCAGG AATATTGGGA TACCATAAGG CCCTTACTTC AGAGGTGGTG TGGAGATCCT 
GCCTTACTCA ACTCTTTGAA GCAGAAAAGT GCTCTGGTCA GGTACCCTAG AAAAAGAAAT 
AGTTTGATAG AGCTTCCTGA TGACTACAGC TGTCTTCTAA ATCAAGCTTC TCACTTTAGG 
TGTCCACGGT CTTCAGATGA TGAACGAAAG CACCCTGTCC TCTGTCTTTT CTGTGGGGCC 
ATCCTGTGTT CTCAGAACAT CTGTTGCCAA GAAATCGTGA ATGGGGAGGA GGTTGGAGCG 
TGCGTTTTTC ATGCACTTCA GTGTGGTGCT GGAGTCTGCA TTTTCCTAAA AATCAGAGAA 
TGCAGAGTGG TCCTGGTAGA AGGAAAAGCC AGAGGCTGTG CCTACCCAGC CCCTTACTTG 
GATGAATATG GAGAAACAGA CCCAGGGCTA AAGAGGGGAA ACCCACTTCA TTTATCTCGG 
GAGCGGTATC GGAAGCTGCA TTTGGTCTGG CAACAGCACT GCATTATAGA AGAGATTGCT 
CGGAGCCAGG AGACTAATCA GATGCTGTTT GGATTCAACT GGCAGTTACT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Role of N-end rule ubiquitin ligases UBR1 and UBR2 in regulating the leucine-mTOR signaling pathway.
Genes to cells : devoted to molecular & cellular mechanisms15(4)339-49(2010 Apr)
Kume K,Iizumi Y,Shimada M,Ito Y,Kishi T,Yamaguchi Y,Handa H

Female lethality and apoptosis of spermatocytes in mice lacking the UBR2 ubiquitin ligase of the N-end rule pathway.
Molecular and cellular biology23(22)8255-71(2003 Nov)
Kwon YT,Xia Z,An JY,Tasaki T,Davydov IV,Seo JW,Sheng J,Xie Y,Varshavsky A

Construction and analysis of mouse strains lacking the ubiquitin ligase UBR1 (E3alpha) of the N-end rule pathway.
Molecular and cellular biology21(23)8007-21(2001 Dec)
Kwon YT,Xia Z,Davydov IV,Lecker SH,Varshavsky A

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Ubr1 cDNA ORF clone, Rattus norvegicus(Norway rat)

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Target ORF information:

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