Cd177 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cd177
Entrez Gene ID	499099
Full Name	CD177 molecule
Synonyms	RGD1562941
General protein information	Preferred Names CD177 molecule Names CD177 antigen
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q21

mRNA and Protein(s)

mRNA	Protein	Name
XM_006228547.3	XP_006228609.1	CD177 antigen isoform X1
NM_001191948.1	NP_001178877.1	CD177 antigen precursor
XM_008758969.2	XP_008757191.1	CD177 antigen isoform X2

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	LOC711291	XP_001100072.1
Canis lupus familiaris (dog)	LOC612424	XP_005616935.1
Homo sapiens (human)	CD177	NP_065139.2
Pan troglodytes (chimpanzee)	CD177	XP_003316463.2
Mus musculus (house mouse)	Cd177	NP_081138.2
Rattus norvegicus (Norway rat)	Cd177	NP_001178877.1

Gene Ontology
Bibliography

Related articles in PubMed

CD177 modulates human neutrophil migration through activation-mediated integrin and chemoreceptor regulation.
Bai M, Grieshaber-Bouyer R, Wang J, Schmider AB, Wilson ZS, Zeng L, Halyabar O, Godin MD, Nguyen HN, Levescot A, Cunin P, Lefort CT, Soberman RJ, Nigrovic PA
Blood130(19)2092-2100(2017 11)

Characterization of the CD177 interaction with the ANCA antigen proteinase 3.
Jerke U, Marino SF, Daumke O, Kettritz R
Scientific reports743328(2017 02)

Heterogeneity of Human Neutrophil CD177 Expression Results from CD177P1 Pseudogene Conversion.
Wu Z, Liang R, Ohnesorg T, Cho V, Lam W, Abhayaratna WP, Gatenby PA, Perera C, Zhang Y, Whittle B, Sinclair A, Goodnow CC, Field M, Andrews TD, Cook MC
PLoS genetics12(5)e1006067(2016 05)

Differential expression of granulopoiesis related genes in neutrophil subsets distinguished by membrane expression of CD177.
Hu N, Mora-Jensen H, Theilgaard-Mönch K, Doornbos-van der Meer B, Huitema MG, Stegeman CA, Heeringa P, Kallenberg CG, Westra J
PloS one9(6)e99671(2014)

Surface receptor CD177/NB1 does not confer a recruitment advantage to neutrophilic granulocytes during human peritonitis.
Wang L, Ge S, Agustian A, Hiss M, Haller H, von Vietinghoff S
European journal of haematology90(5)436-7(2013 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Cd177 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cd177 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa08099	NM_001191948.1 Latest version!	Rattus norvegicus CD177 molecule (Cd177), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ORa49174	XM_008758969.2 Latest version!	Rattus norvegicus CD177 molecule (Cd177), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ORa22465	XM_006228547.3 Latest version!	Rattus norvegicus CD177 molecule (Cd177), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cd177 ( NM_001191948.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001178877.1 Rattus norvegicus CD177 molecule (Cd177), mRNA.

Price & Availability↑

CloneID	ORa08099
Clone ID Related Accession (Same CDS sequence)	NM_001191948.1
Accession Version	NM_001191948.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2457bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1507305600000
Organism	Rattus norvegicus(Norway rat)
Product	CD177 antigen precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AABR07002739.1 and AABR07002737.1. On Jul 17, 2010 this sequence version replaced XM_574390.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN01906355 [ECO:0000350] ##Evidence-Data-END##

ATGAATCCTA TGCCAGTGCT GACCCTCCTG GGGGTCAGCA CCTTGCTACC CTGTGTCCCA 
GCTCTGATCT GCCAGAGAGG CAGAATACAG ACCGTGAGGA ACGAGTCAGA GCTGCCCCTC 
GAGTGGGGGA CGGGTGAGAA AGCTTGCGAG GTCGGCGAGG GTTGCCAAGA TGTGGTGATG 
CTCCTACACA ATGGACCCCT GATCAACTTG GTGATCATCA AGGGCTGCAT AAAGGCTGAG 
GACCAAGAGC CTCGGGTTAC CTGGCTCAGT ACAGGCCCTG GGCTCTCTGT CGTCTCCTAC 
ACACATGTGT GTCGCCATGG TGACCTCTGT AACAATGCAT CCAGCACCAG GATCCTTGAG 
GACCTATCCA CACCCACAGT CCCAGGGTCC CTGCGCTGCC CGCTCTGCCT TTCTAACAAT 
GACTGTGAGA ATGCACCAGA ACAGGTCTGC CCTGTAGGAA GCATATACTG CTACAATGGA 
GTCCTCAGGC TCAGGGGAGA AGGCATCAGG ACCAATCTCA AGGTCCAGGG CTGCATGGCC 
CAGCCAGGCT GCAACCTGCT CAATGGCACC ACAGCAGTTG GGACCTTGGA TATGAGTGAA 
AACTGTGGTC TTCAGTTAGG TCCGCAGGCT CTGGACTGCA ACAGTGCGAG TTTGGACATT 
GTGAAGGACG TATCTGATCT GCACCTGAGC TGGACGACTG GCTGGAAAAC CTGCGAGGCT 
GACGAGGGGT GTTACGAAAC AGTCATGCTA ATACAGAATG GACAAGAAAT TCATATGGCT 
CTCACTAAGG GATGTACTAG CAGTGTAAAC AGAGAGGCTC AACTCACCAG GCATAGAACA 
GGTCCGGGAA TCTCCATCGT CTCCTACGTC CATGTATGTC GCAACGGGGA CTTCTGTAAT 
GACCTGTCTT CAACGGAAAC TCTCTGGATC CCGCCCCCTG ACACAGTCCC AGGGACTCTG 
CGCTGCCCAC ACTGCCTTTC AACGCGTGAC TGTGAGAATG CACCGGAGCA GGTCTGTCCT 
GAAGGCACAC ACTGCTACAA TGGAGTCCTC AGGCTCCGGG GAGGAGGGTT CATCTCTGAT 
CTGAAGGTAC AGGGATGCAC GTCCCAGTCC CAGTCCCAGT CAGACTGCAA CCTGCTCAAC 
GGCATCCAGA CAATCGGACC CATAAAACTA CATGAGAGCT GTGGTTTTCA TTTAGAGTCT 
CTGAAATGCC AGCATGGGAC ACTGGAGGCA GTGCAGCATA TATCGAAACT TCCTCTCCAG 
TGGACAGCTG AGCAGAAAAC CTGTAATGTA GGTGAAGGCT GCCAAGACAC GCTGGTGATA 
ATAGAGAACG GAGAGCACGT GAATTTGGTT CTCACAAAGG GCTGCACTGC CGCTGAGGAC 
CACAAGGCCA AAGTCACGGA ACACAGGACT GGCCCGGGGC TGGCTGTCAT CTCCTACACC 
CGAGTATGCC GTGAAAAAGA CTTATGCAAT GACCTGTCTA CCACAGTCCC TCTCTGGGCT 
CCACCTCCGG TGACAGCCCC AGGGAACACA CGTTGTCCTC TCTGTTTTAC TGCACAAGCC 
TGCGAGAATG CTCCAGAGCA GTTCTGCCCT GCAGGCAGCA CGCACTGCTA CAGTGGAGTC 
CTCAGCCTCA GGGGAGGAGA GATCATCTCC AAACTCAAGG TGCAGGGCTG TATGTCCCAG 
CCAGGCTGCA ACCTGCTCAA TGGCACACAG AAAATCGGAC CTGTGGATGT GAGTGAGGAC 
TGCAGCCCTC GGTCAAATTC TCTGACCTGT CACAGGGGGA CCATGTTGAA GATTGGCAAT 
GGCTTTGCTG AGAAAGCTGT CGAGTGGACT CCACTGTCGT CTCAGGTGTG TGAACCTGAT 
GAGATATGTC AGGAGACGCT GCTGCTCATA GATGTAGGAC AAAAATCAGC CCTCCTGGGG 
AGCAAAGGCT GCAGCAGTCT TGGAGCACAG AACGATGTTG GTGTCTCCAT ATTCTCCCGG 
CCACCTGGGA TGGTGGTAGC TTCCTATACC CGGTTCTGTT CCTCCAACCT GTGCAATGCA 
GCCAGCAGCA CCAGCGTCCT TCTAAGCAAC CTCCCTCGTC CAGAGGTCCT TCCCCCAGGA 
AGTGTGAAGT GTCCCGTGTG TGTACAGTTA TTCAGATCCT GCTTTCAGAC GTCTGACTTT 
GTCACCTGTC CTCGGGGCGC CACTCACTGT TATAGAGGTG ACATCGCGCT AAGAGGAGGT 
GGACTGTCTT CCACATTGAG CATTCAGGGG TGCATGGCCT CACCTGTCAA ATCATTATTG 
GGTAACTCCA AAACAATTGG GATCCTCTCG GCAAAGGAGA GCTCTGAGAA TGAGCGTGGG 
GATGAAGATG ATGAGAAGCC TCTTCTTGAT GGTGCCTCAG CCTCCTCCCC GGCCTCCATG 
TTAGGGCTCC TGGTTCTTCT CAGCTCTTTG TGTGCTGGAA TTTGTCCTCT CCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001178877.1
CDS	1..2457
Translation	MNPMPVLTLLGVSTLLPCVPALICQRGRIQTVRNESELPLEWGTGEKACEVGEGCQDVVMLLHNGPLINLVIIKGCIKAEDQEPRVTWLSTGPGLSVVSYTHVCRHGDLCNNASSTRILEDLSTPTVPGSLRCPLCLSNNDCENAPEQVCPVGSIYCYNGVLRLRGEGIRTNLKVQGCMAQPGCNLLNGTTAVGTLDMSENCGLQLGPQALDCNSASLDIVKDVSDLHLSWTTGWKTCEADEGCYETVMLIQNGQEIHMALTKGCTSSVNREAQLTRHRTGPGISIVSYVHVCRNGDFCNDLSSTETLWIPPPDTVPGTLRCPHCLSTRDCENAPEQVCPEGTHCYNGVLRLRGGGFISDLKVQGCTSQSQSQSDCNLLNGIQTIGPIKLHESCGFHLESLKCQHGTLEAVQHISKLPLQWTAEQKTCNVGEGCQDTLVIIENGEHVNLVLTKGCTAAEDHKAKVTEHRTGPGLAVISYTRVCREKDLCNDLSTTVPLWAPPPVTAPGNTRCPLCFTAQACENAPEQFCPAGSTHCYSGVLSLRGGEIISKLKVQGCMSQPGCNLLNGTQKIGPVDVSEDCSPRSNSLTCHRGTMLKIGNGFAEKAVEWTPLSSQVCEPDEICQETLLLIDVGQKSALLGSKGCSSLGAQNDVGVSIFSRPPGMVVASYTRFCSSNLCNAASSTSVLLSNLPRPEVLPPGSVKCPVCVQLFRSCFQTSDFVTCPRGATHCYRGDIALRGGGLSSTLSIQGCMASPVKSLLGNSKTIGILSAKESSENERGDEDDEKPLLDGASASSPASMLGLLVLLSSLCAGICPLH

Target ORF information:

RefSeq Version	NM_001191948.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus CD177 molecule (Cd177), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001191948.1

ORF Insert Sequence:

ATGAATCCTA TGCCAGTGCT GACCCTCCTG GGGGTCAGCA CCTTGCTACC CTGTGTCCCA 
GCTCTGATCT GCCAGAGAGG CAGAATACAG ACCGTGAGGA ACGAGTCAGA GCTGCCCCTC 
GAGTGGGGGA CGGGTGAGAA AGCTTGCGAG GTCGGCGAGG GTTGCCAAGA TGTGGTGATG 
CTCCTACACA ATGGACCCCT GATCAACTTG GTGATCATCA AGGGCTGCAT AAAGGCTGAG 
GACCAAGAGC CTCGGGTTAC CTGGCTCAGT ACAGGCCCTG GGCTCTCTGT CGTCTCCTAC 
ACACATGTGT GTCGCCATGG TGACCTCTGT AACAATGCAT CCAGCACCAG GATCCTTGAG 
GACCTATCCA CACCCACAGT CCCAGGGTCC CTGCGCTGCC CGCTCTGCCT TTCTAACAAT 
GACTGTGAGA ATGCACCAGA ACAGGTCTGC CCTGTAGGAA GCATATACTG CTACAATGGA 
GTCCTCAGGC TCAGGGGAGA AGGCATCAGG ACCAATCTCA AGGTCCAGGG CTGCATGGCC 
CAGCCAGGCT GCAACCTGCT CAATGGCACC ACAGCAGTTG GGACCTTGGA TATGAGTGAA 
AACTGTGGTC TTCAGTTAGG TCCGCAGGCT CTGGACTGCA ACAGTGCGAG TTTGGACATT 
GTGAAGGACG TATCTGATCT GCACCTGAGC TGGACGACTG GCTGGAAAAC CTGCGAGGCT 
GACGAGGGGT GTTACGAAAC AGTCATGCTA ATACAGAATG GACAAGAAAT TCATATGGCT 
CTCACTAAGG GATGTACTAG CAGTGTAAAC AGAGAGGCTC AACTCACCAG GCATAGAACA 
GGTCCGGGAA TCTCCATCGT CTCCTACGTC CATGTATGTC GCAACGGGGA CTTCTGTAAT 
GACCTGTCTT CAACGGAAAC TCTCTGGATC CCGCCCCCTG ACACAGTCCC AGGGACTCTG 
CGCTGCCCAC ACTGCCTTTC AACGCGTGAC TGTGAGAATG CACCGGAGCA GGTCTGTCCT 
GAAGGCACAC ACTGCTACAA TGGAGTCCTC AGGCTCCGGG GAGGAGGGTT CATCTCTGAT 
CTGAAGGTAC AGGGATGCAC GTCCCAGTCC CAGTCCCAGT CAGACTGCAA CCTGCTCAAC 
GGCATCCAGA CAATCGGACC CATAAAACTA CATGAGAGCT GTGGTTTTCA TTTAGAGTCT 
CTGAAATGCC AGCATGGGAC ACTGGAGGCA GTGCAGCATA TATCGAAACT TCCTCTCCAG 
TGGACAGCTG AGCAGAAAAC CTGTAATGTA GGTGAAGGCT GCCAAGACAC GCTGGTGATA 
ATAGAGAACG GAGAGCACGT GAATTTGGTT CTCACAAAGG GCTGCACTGC CGCTGAGGAC 
CACAAGGCCA AAGTCACGGA ACACAGGACT GGCCCGGGGC TGGCTGTCAT CTCCTACACC 
CGAGTATGCC GTGAAAAAGA CTTATGCAAT GACCTGTCTA CCACAGTCCC TCTCTGGGCT 
CCACCTCCGG TGACAGCCCC AGGGAACACA CGTTGTCCTC TCTGTTTTAC TGCACAAGCC 
TGCGAGAATG CTCCAGAGCA GTTCTGCCCT GCAGGCAGCA CGCACTGCTA CAGTGGAGTC 
CTCAGCCTCA GGGGAGGAGA GATCATCTCC AAACTCAAGG TGCAGGGCTG TATGTCCCAG 
CCAGGCTGCA ACCTGCTCAA TGGCACACAG AAAATCGGAC CTGTGGATGT GAGTGAGGAC 
TGCAGCCCTC GGTCAAATTC TCTGACCTGT CACAGGGGGA CCATGTTGAA GATTGGCAAT 
GGCTTTGCTG AGAAAGCTGT CGAGTGGACT CCACTGTCGT CTCAGGTGTG TGAACCTGAT 
GAGATATGTC AGGAGACGCT GCTGCTCATA GATGTAGGAC AAAAATCAGC CCTCCTGGGG 
AGCAAAGGCT GCAGCAGTCT TGGAGCACAG AACGATGTTG GTGTCTCCAT ATTCTCCCGG 
CCACCTGGGA TGGTGGTAGC TTCCTATACC CGGTTCTGTT CCTCCAACCT GTGCAATGCA 
GCCAGCAGCA CCAGCGTCCT TCTAAGCAAC CTCCCTCGTC CAGAGGTCCT TCCCCCAGGA 
AGTGTGAAGT GTCCCGTGTG TGTACAGTTA TTCAGATCCT GCTTTCAGAC GTCTGACTTT 
GTCACCTGTC CTCGGGGCGC CACTCACTGT TATAGAGGTG ACATCGCGCT AAGAGGAGGT 
GGACTGTCTT CCACATTGAG CATTCAGGGG TGCATGGCCT CACCTGTCAA ATCATTATTG 
GGTAACTCCA AAACAATTGG GATCCTCTCG GCAAAGGAGA GCTCTGAGAA TGAGCGTGGG 
GATGAAGATG ATGAGAAGCC TCTTCTTGAT GGTGCCTCAG CCTCCTCCCC GGCCTCCATG 
TTAGGGCTCC TGGTTCTTCT CAGCTCTTTG TGTGCTGGAA TTTGTCCTCT CCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cd177 ( XM_008758969.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008757191.1 Rattus norvegicus CD177 molecule (Cd177), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa49174
Clone ID Related Accession (Same CDS sequence)	XM_008758969.2
Accession Version	XM_008758969.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2457bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	CD177 antigen isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008758969.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCCTA TGCCAGTGCT GACCCTCCTG GGGGTCAGCA CCTTGCTACC CTGTGTCCCA 
GCTCTGATCT GCCAGAGAGG CAGAATACAG ACCGTGAGGA ACGAGTCAGA GCTGCCCCTC 
GAGTGGGGGA CGGGTGAGAA AGCTTGCGAG GTCGGCGAGG GTTGCCAAGA TGTGGTGATG 
CTCCTACACA ATGGACCCCT GATCAACTTG GTGATCATCA AGGGCTGCAT AAAGGCTGAG 
GACCAAGAGC CTCGGGTTAC CTGGCTCAGT ACAGGCCCTG GGCTCTCTGT CGTCTCCTAC 
ACACATGTGT GTCGCCATGG TGACCTCTGT AACAATGCAT CCAGCACCAG GATCCTTGAG 
GACCTATCCA CACCCACAGT CCCAGGGTCC CTGCGCTGCC CGCTCTGCCT TTCTAACAAT 
GACTGTGAGA ATGCACCAGA ACAGGTCTGC CCTGTAGGAA GCATATACTG CTACAATGGA 
GTCCTCAGGC TCAGGGGAGA AGGCATCAGG ACCAATCTCA AGGTCCAGGG CTGCATGGCC 
CAGCCAGGCT GCAACCTGCT CAATGGCACC ACAGCAGTTG GGACCTTGGA TATGAGTGAA 
AACTGTGGTC TTCAGTTAGG TCCGCAGGCT CTGGACTGCA ACAGTGCGAG TTTGGACATT 
GTGAAGGACG TATCTGATCT GCACCTGAGC TGGACGACTG GCTGGAAAAC CTGCGAGGCT 
GACGAGGGGT GTTACGAAAC AGTCATGCTA ATACAGAATG GACAAGAAAT TCATATGGCT 
CTCACTAAGG GATGTACTAG CAGTGTAAAC AGAGAGGCTC AACTCACCAG GCATAGAACA 
GGTCCGGGAA TCTCCATCGT CTCCTACGTC CATGTATGTC GCAACGGGGA CTTCTGTAAT 
GACCTGTCTT CAACGGAAAC TCTCTGGATC CCGCCCCCTG ACACAGTCCC AGGGACTCTG 
CGCTGCCCAC ACTGCCTTTC AACGCGTGAC TGTGAGAATG CACCGGAGCA GGTCTGTCCT 
GAAGGCACAC ACTGCTACAA TGGAGTCCTC AGGCTCCGGG GAGGGGCCAT TGCCACAAAT 
TTGAGAGTGC AGGGCTGCAC TTCACAGCCA GGCTGTGGAC TGCTCAATGG CACCAAGACA 
ATTGGACCTA TAGGTGTGCG TGAGGACTGC GGTCCCCAGT CAGGCGCTCA GGCACTAGAA 
TGCCGGAGTG GGTGGGCAGA AGGCCTAAGG AAAGTGTCCT CACTGCCCCT GGACTGGACA 
ACCGCCTGGG AGACTTGTAA AATCGGCGAA GGGTGCCAGG AAACTGCCTT GCTCATACAG 
AATGGACCTC TTGTGAACCT GGTGCTCATC AAAGGCTGCA CAGCAGAAGA AGACCACAAG 
CCCCAAATCA CTCGGCATAG GCTGAGTCCC AGCCTATCCA TCGTGTCTTA TAGCCATGTG 
TGCCGCCACT GGGACTTCTG CAATGACCTG TCCTCCACCC ACCCTCTCTG GCTCCCACCA 
CCACCTGTCG AAGGCCTGGG GAATGTGAGC TGCCCATTCT GCCTTTCTAA AGATGGCTGT 
CCTCAGAACG GACCCAAGCA GGTCTGCCCT GTAGACAGTT CACACTGCTA CAGTGGAGTC 
CTCAGCCTCA GGGGAGGAGA GATCATCTCC AAACTCAAGG TGCAGGGCTG TATGTCCCAG 
CCAGGCTGCA ACCTGCTCAA TGGCACACAG AAAATCGGAC CTGTGGATGT GAGTGAGGAC 
TGCAGCCCTC GGTCAAATTC TCTGACCTGT CACAGGGGGA CCATGTTGAA GATTGGCAAT 
GGCTTTGCTG AGAAAGCTGT CGAGTGGACT CCACTGTCGT CTCAGGTGTG TGAACCTGAT 
GAGATATGTC AGGAGACGCT GCTGCTCATA GATGTAGGAC AAAAATCAGC CCTCCTGGGG 
AGCAAAGGCT GCAGCAGTCT TGGAGCACAG AACGATGTTG GTGTCTCCAT ATTCTCCCGG 
CCACCTGGGA TGGTGGTAGC TTCCTATACC CGGTTCTGTT CCTCCAACCT GTGCAATGCA 
GCCAGCAGCA CCAGCGTCCT TCTAAGCAAC CTCCCTCGTC CAGAGGTCCT TCCCCCAGGA 
AGTGTGAAGT GTCCCGTGTG TGTACAGTTA TTCAGATCCT GCTTTCAGAC GTCTGACTTT 
GTCACCTGTC CTCGGGGCGC CACTCACTGT TATAGAGGTG ACATCGCGCT AAGAGGAGGT 
GGACTGTCTT CCACATTGAG CATTCAGGGG TGCATGGCCT CACCTGTCAA ATCATTATTG 
GGTAACTCCA AAACAATTGG GATCCTCTCG GCAAAGGAGA GCTCTGAGAA TGAGCGTGGG 
GATGAAGATG ATGAGAAGCC TCTTCTTGAT GGTGCCTCAG CCTCCTCCCC GGCCTCCATG 
TTAGGGCTCC TGGTTCTTCT CAGCTCTTTG TGTGCTGGAA TTTGTCCTCT CCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008757191.1
CDS	54..2510
Translation	MNPMPVLTLLGVSTLLPCVPALICQRGRIQTVRNESELPLEWGTGEKACEVGEGCQDVVMLLHNGPLINLVIIKGCIKAEDQEPRVTWLSTGPGLSVVSYTHVCRHGDLCNNASSTRILEDLSTPTVPGSLRCPLCLSNNDCENAPEQVCPVGSIYCYNGVLRLRGEGIRTNLKVQGCMAQPGCNLLNGTTAVGTLDMSENCGLQLGPQALDCNSASLDIVKDVSDLHLSWTTGWKTCEADEGCYETVMLIQNGQEIHMALTKGCTSSVNREAQLTRHRTGPGISIVSYVHVCRNGDFCNDLSSTETLWIPPPDTVPGTLRCPHCLSTRDCENAPEQVCPEGTHCYNGVLRLRGGAIATNLRVQGCTSQPGCGLLNGTKTIGPIGVREDCGPQSGAQALECRSGWAEGLRKVSSLPLDWTTAWETCKIGEGCQETALLIQNGPLVNLVLIKGCTAEEDHKPQITRHRLSPSLSIVSYSHVCRHWDFCNDLSSTHPLWLPPPPVEGLGNVSCPFCLSKDGCPQNGPKQVCPVDSSHCYSGVLSLRGGEIISKLKVQGCMSQPGCNLLNGTQKIGPVDVSEDCSPRSNSLTCHRGTMLKIGNGFAEKAVEWTPLSSQVCEPDEICQETLLLIDVGQKSALLGSKGCSSLGAQNDVGVSIFSRPPGMVVASYTRFCSSNLCNAASSTSVLLSNLPRPEVLPPGSVKCPVCVQLFRSCFQTSDFVTCPRGATHCYRGDIALRGGGLSSTLSIQGCMASPVKSLLGNSKTIGILSAKESSENERGDEDDEKPLLDGASASSPASMLGLLVLLSSLCAGICPLH

Target ORF information:

RefSeq Version	XM_008758969.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus CD177 molecule (Cd177), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008758969.2

ORF Insert Sequence:

ATGAATCCTA TGCCAGTGCT GACCCTCCTG GGGGTCAGCA CCTTGCTACC CTGTGTCCCA 
GCTCTGATCT GCCAGAGAGG CAGAATACAG ACCGTGAGGA ACGAGTCAGA GCTGCCCCTC 
GAGTGGGGGA CGGGTGAGAA AGCTTGCGAG GTCGGCGAGG GTTGCCAAGA TGTGGTGATG 
CTCCTACACA ATGGACCCCT GATCAACTTG GTGATCATCA AGGGCTGCAT AAAGGCTGAG 
GACCAAGAGC CTCGGGTTAC CTGGCTCAGT ACAGGCCCTG GGCTCTCTGT CGTCTCCTAC 
ACACATGTGT GTCGCCATGG TGACCTCTGT AACAATGCAT CCAGCACCAG GATCCTTGAG 
GACCTATCCA CACCCACAGT CCCAGGGTCC CTGCGCTGCC CGCTCTGCCT TTCTAACAAT 
GACTGTGAGA ATGCACCAGA ACAGGTCTGC CCTGTAGGAA GCATATACTG CTACAATGGA 
GTCCTCAGGC TCAGGGGAGA AGGCATCAGG ACCAATCTCA AGGTCCAGGG CTGCATGGCC 
CAGCCAGGCT GCAACCTGCT CAATGGCACC ACAGCAGTTG GGACCTTGGA TATGAGTGAA 
AACTGTGGTC TTCAGTTAGG TCCGCAGGCT CTGGACTGCA ACAGTGCGAG TTTGGACATT 
GTGAAGGACG TATCTGATCT GCACCTGAGC TGGACGACTG GCTGGAAAAC CTGCGAGGCT 
GACGAGGGGT GTTACGAAAC AGTCATGCTA ATACAGAATG GACAAGAAAT TCATATGGCT 
CTCACTAAGG GATGTACTAG CAGTGTAAAC AGAGAGGCTC AACTCACCAG GCATAGAACA 
GGTCCGGGAA TCTCCATCGT CTCCTACGTC CATGTATGTC GCAACGGGGA CTTCTGTAAT 
GACCTGTCTT CAACGGAAAC TCTCTGGATC CCGCCCCCTG ACACAGTCCC AGGGACTCTG 
CGCTGCCCAC ACTGCCTTTC AACGCGTGAC TGTGAGAATG CACCGGAGCA GGTCTGTCCT 
GAAGGCACAC ACTGCTACAA TGGAGTCCTC AGGCTCCGGG GAGGGGCCAT TGCCACAAAT 
TTGAGAGTGC AGGGCTGCAC TTCACAGCCA GGCTGTGGAC TGCTCAATGG CACCAAGACA 
ATTGGACCTA TAGGTGTGCG TGAGGACTGC GGTCCCCAGT CAGGCGCTCA GGCACTAGAA 
TGCCGGAGTG GGTGGGCAGA AGGCCTAAGG AAAGTGTCCT CACTGCCCCT GGACTGGACA 
ACCGCCTGGG AGACTTGTAA AATCGGCGAA GGGTGCCAGG AAACTGCCTT GCTCATACAG 
AATGGACCTC TTGTGAACCT GGTGCTCATC AAAGGCTGCA CAGCAGAAGA AGACCACAAG 
CCCCAAATCA CTCGGCATAG GCTGAGTCCC AGCCTATCCA TCGTGTCTTA TAGCCATGTG 
TGCCGCCACT GGGACTTCTG CAATGACCTG TCCTCCACCC ACCCTCTCTG GCTCCCACCA 
CCACCTGTCG AAGGCCTGGG GAATGTGAGC TGCCCATTCT GCCTTTCTAA AGATGGCTGT 
CCTCAGAACG GACCCAAGCA GGTCTGCCCT GTAGACAGTT CACACTGCTA CAGTGGAGTC 
CTCAGCCTCA GGGGAGGAGA GATCATCTCC AAACTCAAGG TGCAGGGCTG TATGTCCCAG 
CCAGGCTGCA ACCTGCTCAA TGGCACACAG AAAATCGGAC CTGTGGATGT GAGTGAGGAC 
TGCAGCCCTC GGTCAAATTC TCTGACCTGT CACAGGGGGA CCATGTTGAA GATTGGCAAT 
GGCTTTGCTG AGAAAGCTGT CGAGTGGACT CCACTGTCGT CTCAGGTGTG TGAACCTGAT 
GAGATATGTC AGGAGACGCT GCTGCTCATA GATGTAGGAC AAAAATCAGC CCTCCTGGGG 
AGCAAAGGCT GCAGCAGTCT TGGAGCACAG AACGATGTTG GTGTCTCCAT ATTCTCCCGG 
CCACCTGGGA TGGTGGTAGC TTCCTATACC CGGTTCTGTT CCTCCAACCT GTGCAATGCA 
GCCAGCAGCA CCAGCGTCCT TCTAAGCAAC CTCCCTCGTC CAGAGGTCCT TCCCCCAGGA 
AGTGTGAAGT GTCCCGTGTG TGTACAGTTA TTCAGATCCT GCTTTCAGAC GTCTGACTTT 
GTCACCTGTC CTCGGGGCGC CACTCACTGT TATAGAGGTG ACATCGCGCT AAGAGGAGGT 
GGACTGTCTT CCACATTGAG CATTCAGGGG TGCATGGCCT CACCTGTCAA ATCATTATTG 
GGTAACTCCA AAACAATTGG GATCCTCTCG GCAAAGGAGA GCTCTGAGAA TGAGCGTGGG 
GATGAAGATG ATGAGAAGCC TCTTCTTGAT GGTGCCTCAG CCTCCTCCCC GGCCTCCATG 
TTAGGGCTCC TGGTTCTTCT CAGCTCTTTG TGTGCTGGAA TTTGTCCTCT CCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cd177 ( XM_006228547.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006228609.1 Rattus norvegicus CD177 molecule (Cd177), transcript variant X1, mRNA.

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CloneID	ORa22465
Clone ID Related Accession (Same CDS sequence)	XM_006228547.3
Accession Version	XM_006228547.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3036bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	CD177 antigen isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006228547.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCCTA TGCCAGTGCT GACCCTCCTG GGGGTCAGCA CCTTGCTACC CTGTGTCCCA 
GCTCTGATCT GCCAGAGAGG CAGAATACAG ACCGTGAGGA ACGAGTCAGA GCTGCCCCTC 
GAGTGGGGGA CGGGTGAGAA AGCTTGCGAG GTCGGCGAGG GTTGCCAAGA TGTGGTGATG 
CTCCTACACA ATGGACCCCT GATCAACTTG GTGATCATCA AGGGCTGCAT AAAGGCTGAG 
GACCAAGAGC CTCGGGTTAC CTGGCTCAGT ACAGGCCCTG GGCTCTCTGT CGTCTCCTAC 
ACACATGTGT GTCGCCATGG TGACCTCTGT AACAATGCAT CCAGCACCAG GATCCTTGAG 
GACCTATCCA CACCCACAGT CCCAGGGTCC CTGCGCTGCC CGCTCTGCCT TTCTAACAAT 
GACTGTGAGA ATGCACCAGA ACAGGTCTGC CCTGTAGGAA GCATATACTG CTACAATGGA 
GTCCTCAGGC TCAGGGGAGA AGGCATCAGG ACCAATCTCA AGGTCCAGGG CTGCATGGCC 
CAGCCAGGCT GCAACCTGCT CAATGGCACC ACAGCAGTTG GGACCTTGGA TATGAGTGAA 
AACTGTGGTC TTCAGTTAGG TCCGCAGGCT CTGGACTGCA ACAGTGCGAG TTTGGACATT 
GTGAAGGACG TATCTGATCT GCACCTGAGC TGGACGACTG GCTGGAAAAC CTGCGAGGCT 
GACGAGGGGT GTTACGAAAC AGTCATGCTA ATACAGAATG GACAAGAAAT TCATATGGCT 
CTCACTAAGG GATGTACTAG CAGTGTAAAC AGAGAGGCTC AACTCACCAG GCATAGAACA 
GGTCCGGGAA TCTCCATCGT CTCCTACGTC CATGTATGTC GCAACGGGGA CTTCTGTAAT 
GACCTGTCTT CAACGGAAAC TCTCTGGATC CCGCCCCCTG ACACAGTCCC AGGGACTCTG 
CGCTGCCCAC ACTGCCTTTC AACGCGTGAC TGTGAGAATG CACCGGAGCA GGTCTGTCCT 
GAAGGCACAC ACTGCTACAA TGGAGTCCTC AGGCTCCGGG GAGGGGCCAT TGCCACAAAT 
TTGAGAGTGC AGGGCTGCAC TTCACAGCCA GGCTGTGGAC TGCTCAATGG CACCAAGACA 
ATTGGACCTA TAGGTGTGCG TGAGGACTGC GGTCCCCAGT CAGGCGCTCA GGCACTAGAA 
TGCCGGAGTG GGTGGGCAGA AGGCCTAAGG AAAGTGTCCT CACTGCCCCT GGACTGGACA 
ACCGCCTGGG AGACTTGTAA AATCGGCGAA GGGTGCCAGG AAACTGCCTT GCTCATACAG 
AATGGACCTC TTGTGAACCT GGTGCTCATC AAAGGCTGCA CAGCAGAAGA AGACCACAAG 
CCCCAAATCA CTCGGCATAG GCTGAGTCCC AGCCTATCCA TCGTGTCTTA TAGCCATGTG 
TGCCGCCACT GGGACTTCTG CAATGACCTG TCCTCCACCC ACCCTCTCTG GCTCCCACCA 
CCACCTGTCG AAGGCCTGGG GAATGTGAGC TGCCCATTCT GCCTTTCTAA AGATGGCTGT 
CCTCAGAACG GACCCAAGCA GGTCTGCCCT GTAGACAGTT CACACTGCTA CAGTGGAGTC 
CTCAGCCTCA GGGGAGGAGG GTTCATCTCT GATCTGAAGG TACAGGGATG CACGTCCCAG 
TCCCAGTCCC AGTCAGACTG CAACCTGCTC AACGGCATCC AGACAATCGG ACCCATAAAA 
CTACATGAGA GCTGTGGTTT TCATTTAGCT GCAGAGTCTC TGAAATGCCA GCATGGGACA 
CTGGAGGCAG TGCAGCATAT ATCGAAACTT CCTCTCCAGT GGACAGCTGA GCAGAAAACC 
TGTAATGTAG GTGAAGGCTG CCAAGACACG CTGGTGATAA TAGAGAACGG AGAGCACGTG 
AATTTGGTTC TCACAAAGGG CTGCACTGCC GCTGAGGACC ACAAGGCCAA AGTCACGGAA 
CACAGGACTG GCCCGGGGCT GGCTGTCATC TCCTACACCC GAGTATGCCG TGAAAAAGAC 
TTATGCAATG ACCTGTCTAC CACAGTCCCT CTCTGGGCTC CACCTCCGGT GACAGCCCCA 
GGGAACACAC GTTGTCCTCT CTGTTTTACT GCACAAGCCT GCGAGAATGC TCCAGAGCAG 
TTCTGCCCTG CAGGCAGCAC GCACTGCTAC AGTGGAGTCC TCAGCCTCAG GGGAGGAGAG 
ATCATCTCCA AACTCAAGGT GCAGGGCTGT ATGTCCCAGC CAGGCTGCAA CCTGCTCAAT 
GGCACACAGA AAATCGGACC TGTGGATGTG AGTGAGGACT GCAGCCCTCG GTCAAATTCT 
CTGACCTGTC ACAGGGGGAC CATGTTGAAG ATTGGCAATG GCTTTGCTGA GAAAGCTGTC 
GAGTGGACTC CACTGTCGTC TCAGGTGTGT GAACCTGATG AGATATGTCA GGAGACGCTG 
CTGCTCATAG ATGTAGGACA AAAATCAGCC CTCCTGGGGA GCAAAGGCTG CAGCAGTCTT 
GGAGCACAGA ACGATGTTGG TGTCTCCATA TTCTCCCGGC CACCTGGGAT GGTGGTAGCT 
TCCTATACCC GGTTCTGTTC CTCCAACCTG TGCAATGCAG CCAGCAGCAC CAGCGTCCTT 
CTAAGCAACC TCCCTCGTCC AGAGGTCCTT CCCCCAGGAA GTGTGAAGTG TCCCGTGTGT 
GTACAGTTAT TCAGATCCTG CTTTCAGACG TCTGACTTTG TCACCTGTCC TCGGGGCGCC 
ACTCACTGTT ATAGAGGTGA CATCGCGCTA AGAGGAGGTG GACTGTCTTC CACATTGAGC 
ATTCAGGGGT GCATGGCCTC ACCTGTCAAA TCATTATTGG GTAACTCCAA AACAATTGGG 
ATCCTCTCGG CAAAGGAGAG CTCTGAGAAT GAGCGTGGGG ATGAAGATGA TGAGAAGCCT 
CTTCTTGATG GTGCCTCAGC CTCCTCCCCG GCCTCCATGT TAGGGCTCCT GGTTCTTCTC 
AGCTCTTTGT GTGCTGGAAT TTGTCCTCTC CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006228609.1
CDS	53..3088
Translation	MNPMPVLTLLGVSTLLPCVPALICQRGRIQTVRNESELPLEWGTGEKACEVGEGCQDVVMLLHNGPLINLVIIKGCIKAEDQEPRVTWLSTGPGLSVVSYTHVCRHGDLCNNASSTRILEDLSTPTVPGSLRCPLCLSNNDCENAPEQVCPVGSIYCYNGVLRLRGEGIRTNLKVQGCMAQPGCNLLNGTTAVGTLDMSENCGLQLGPQALDCNSASLDIVKDVSDLHLSWTTGWKTCEADEGCYETVMLIQNGQEIHMALTKGCTSSVNREAQLTRHRTGPGISIVSYVHVCRNGDFCNDLSSTETLWIPPPDTVPGTLRCPHCLSTRDCENAPEQVCPEGTHCYNGVLRLRGGAIATNLRVQGCTSQPGCGLLNGTKTIGPIGVREDCGPQSGAQALECRSGWAEGLRKVSSLPLDWTTAWETCKIGEGCQETALLIQNGPLVNLVLIKGCTAEEDHKPQITRHRLSPSLSIVSYSHVCRHWDFCNDLSSTHPLWLPPPPVEGLGNVSCPFCLSKDGCPQNGPKQVCPVDSSHCYSGVLSLRGGGFISDLKVQGCTSQSQSQSDCNLLNGIQTIGPIKLHESCGFHLAAESLKCQHGTLEAVQHISKLPLQWTAEQKTCNVGEGCQDTLVIIENGEHVNLVLTKGCTAAEDHKAKVTEHRTGPGLAVISYTRVCREKDLCNDLSTTVPLWAPPPVTAPGNTRCPLCFTAQACENAPEQFCPAGSTHCYSGVLSLRGGEIISKLKVQGCMSQPGCNLLNGTQKIGPVDVSEDCSPRSNSLTCHRGTMLKIGNGFAEKAVEWTPLSSQVCEPDEICQETLLLIDVGQKSALLGSKGCSSLGAQNDVGVSIFSRPPGMVVASYTRFCSSNLCNAASSTSVLLSNLPRPEVLPPGSVKCPVCVQLFRSCFQTSDFVTCPRGATHCYRGDIALRGGGLSSTLSIQGCMASPVKSLLGNSKTIGILSAKESSENERGDEDDEKPLLDGASASSPASMLGLLVLLSSLCAGICPLH

Target ORF information:

RefSeq Version	XM_006228547.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus CD177 molecule (Cd177), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006228547.3

ORF Insert Sequence:

ATGAATCCTA TGCCAGTGCT GACCCTCCTG GGGGTCAGCA CCTTGCTACC CTGTGTCCCA 
GCTCTGATCT GCCAGAGAGG CAGAATACAG ACCGTGAGGA ACGAGTCAGA GCTGCCCCTC 
GAGTGGGGGA CGGGTGAGAA AGCTTGCGAG GTCGGCGAGG GTTGCCAAGA TGTGGTGATG 
CTCCTACACA ATGGACCCCT GATCAACTTG GTGATCATCA AGGGCTGCAT AAAGGCTGAG 
GACCAAGAGC CTCGGGTTAC CTGGCTCAGT ACAGGCCCTG GGCTCTCTGT CGTCTCCTAC 
ACACATGTGT GTCGCCATGG TGACCTCTGT AACAATGCAT CCAGCACCAG GATCCTTGAG 
GACCTATCCA CACCCACAGT CCCAGGGTCC CTGCGCTGCC CGCTCTGCCT TTCTAACAAT 
GACTGTGAGA ATGCACCAGA ACAGGTCTGC CCTGTAGGAA GCATATACTG CTACAATGGA 
GTCCTCAGGC TCAGGGGAGA AGGCATCAGG ACCAATCTCA AGGTCCAGGG CTGCATGGCC 
CAGCCAGGCT GCAACCTGCT CAATGGCACC ACAGCAGTTG GGACCTTGGA TATGAGTGAA 
AACTGTGGTC TTCAGTTAGG TCCGCAGGCT CTGGACTGCA ACAGTGCGAG TTTGGACATT 
GTGAAGGACG TATCTGATCT GCACCTGAGC TGGACGACTG GCTGGAAAAC CTGCGAGGCT 
GACGAGGGGT GTTACGAAAC AGTCATGCTA ATACAGAATG GACAAGAAAT TCATATGGCT 
CTCACTAAGG GATGTACTAG CAGTGTAAAC AGAGAGGCTC AACTCACCAG GCATAGAACA 
GGTCCGGGAA TCTCCATCGT CTCCTACGTC CATGTATGTC GCAACGGGGA CTTCTGTAAT 
GACCTGTCTT CAACGGAAAC TCTCTGGATC CCGCCCCCTG ACACAGTCCC AGGGACTCTG 
CGCTGCCCAC ACTGCCTTTC AACGCGTGAC TGTGAGAATG CACCGGAGCA GGTCTGTCCT 
GAAGGCACAC ACTGCTACAA TGGAGTCCTC AGGCTCCGGG GAGGGGCCAT TGCCACAAAT 
TTGAGAGTGC AGGGCTGCAC TTCACAGCCA GGCTGTGGAC TGCTCAATGG CACCAAGACA 
ATTGGACCTA TAGGTGTGCG TGAGGACTGC GGTCCCCAGT CAGGCGCTCA GGCACTAGAA 
TGCCGGAGTG GGTGGGCAGA AGGCCTAAGG AAAGTGTCCT CACTGCCCCT GGACTGGACA 
ACCGCCTGGG AGACTTGTAA AATCGGCGAA GGGTGCCAGG AAACTGCCTT GCTCATACAG 
AATGGACCTC TTGTGAACCT GGTGCTCATC AAAGGCTGCA CAGCAGAAGA AGACCACAAG 
CCCCAAATCA CTCGGCATAG GCTGAGTCCC AGCCTATCCA TCGTGTCTTA TAGCCATGTG 
TGCCGCCACT GGGACTTCTG CAATGACCTG TCCTCCACCC ACCCTCTCTG GCTCCCACCA 
CCACCTGTCG AAGGCCTGGG GAATGTGAGC TGCCCATTCT GCCTTTCTAA AGATGGCTGT 
CCTCAGAACG GACCCAAGCA GGTCTGCCCT GTAGACAGTT CACACTGCTA CAGTGGAGTC 
CTCAGCCTCA GGGGAGGAGG GTTCATCTCT GATCTGAAGG TACAGGGATG CACGTCCCAG 
TCCCAGTCCC AGTCAGACTG CAACCTGCTC AACGGCATCC AGACAATCGG ACCCATAAAA 
CTACATGAGA GCTGTGGTTT TCATTTAGCT GCAGAGTCTC TGAAATGCCA GCATGGGACA 
CTGGAGGCAG TGCAGCATAT ATCGAAACTT CCTCTCCAGT GGACAGCTGA GCAGAAAACC 
TGTAATGTAG GTGAAGGCTG CCAAGACACG CTGGTGATAA TAGAGAACGG AGAGCACGTG 
AATTTGGTTC TCACAAAGGG CTGCACTGCC GCTGAGGACC ACAAGGCCAA AGTCACGGAA 
CACAGGACTG GCCCGGGGCT GGCTGTCATC TCCTACACCC GAGTATGCCG TGAAAAAGAC 
TTATGCAATG ACCTGTCTAC CACAGTCCCT CTCTGGGCTC CACCTCCGGT GACAGCCCCA 
GGGAACACAC GTTGTCCTCT CTGTTTTACT GCACAAGCCT GCGAGAATGC TCCAGAGCAG 
TTCTGCCCTG CAGGCAGCAC GCACTGCTAC AGTGGAGTCC TCAGCCTCAG GGGAGGAGAG 
ATCATCTCCA AACTCAAGGT GCAGGGCTGT ATGTCCCAGC CAGGCTGCAA CCTGCTCAAT 
GGCACACAGA AAATCGGACC TGTGGATGTG AGTGAGGACT GCAGCCCTCG GTCAAATTCT 
CTGACCTGTC ACAGGGGGAC CATGTTGAAG ATTGGCAATG GCTTTGCTGA GAAAGCTGTC 
GAGTGGACTC CACTGTCGTC TCAGGTGTGT GAACCTGATG AGATATGTCA GGAGACGCTG 
CTGCTCATAG ATGTAGGACA AAAATCAGCC CTCCTGGGGA GCAAAGGCTG CAGCAGTCTT 
GGAGCACAGA ACGATGTTGG TGTCTCCATA TTCTCCCGGC CACCTGGGAT GGTGGTAGCT 
TCCTATACCC GGTTCTGTTC CTCCAACCTG TGCAATGCAG CCAGCAGCAC CAGCGTCCTT 
CTAAGCAACC TCCCTCGTCC AGAGGTCCTT CCCCCAGGAA GTGTGAAGTG TCCCGTGTGT 
GTACAGTTAT TCAGATCCTG CTTTCAGACG TCTGACTTTG TCACCTGTCC TCGGGGCGCC 
ACTCACTGTT ATAGAGGTGA CATCGCGCTA AGAGGAGGTG GACTGTCTTC CACATTGAGC 
ATTCAGGGGT GCATGGCCTC ACCTGTCAAA TCATTATTGG GTAACTCCAA AACAATTGGG 
ATCCTCTCGG CAAAGGAGAG CTCTGAGAAT GAGCGTGGGG ATGAAGATGA TGAGAAGCCT 
CTTCTTGATG GTGCCTCAGC CTCCTCCCCG GCCTCCATGT TAGGGCTCCT GGTTCTTCTC 
AGCTCTTTGT GTGCTGGAAT TTGTCCTCTC CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

CD177 modulates human neutrophil migration through activation-mediated integrin and chemoreceptor regulation.
Blood130(19)2092-2100(2017 11)
Bai M,Grieshaber-Bouyer R,Wang J,Schmider AB,Wilson ZS,Zeng L,Halyabar O,Godin MD,Nguyen HN,Levescot A,Cunin P,Lefort CT,Soberman RJ,Nigrovic PA

Characterization of the CD177 interaction with the ANCA antigen proteinase 3.
Scientific reports743328(2017 02)
Jerke U,Marino SF,Daumke O,Kettritz R

Heterogeneity of Human Neutrophil CD177 Expression Results from CD177P1 Pseudogene Conversion.
PLoS genetics12(5)e1006067(2016 05)
Wu Z,Liang R,Ohnesorg T,Cho V,Lam W,Abhayaratna WP,Gatenby PA,Perera C,Zhang Y,Whittle B,Sinclair A,Goodnow CC,Field M,Andrews TD,Cook MC

Differential expression of granulopoiesis related genes in neutrophil subsets distinguished by membrane expression of CD177.
PloS one9(6)e99671(2014)
Hu N,Mora-Jensen H,Theilgaard-Mönch K,Doornbos-van der Meer B,Huitema MG,Stegeman CA,Heeringa P,Kallenberg CG,Westra J

Surface receptor CD177/NB1 does not confer a recruitment advantage to neutrophilic granulocytes during human peritonitis.
European journal of haematology90(5)436-7(2013 May)
Wang L,Ge S,Agustian A,Hiss M,Haller H,von Vietinghoff S

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Cd177 cDNA ORF clone, Rattus norvegicus(Norway rat)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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