Usp9x cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Usp9x
Entrez Gene ID	363445
Full Name	ubiquitin specific peptidase 9, X-linked
Synonyms	RGD1560056
General protein information	Preferred Names ubiquitin specific peptidase 9, X-linked Names probable ubiquitin carboxyl-terminal hydrolase FAF-X ubiquitin specific peptidase 9, X chromosome
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: X Map Location: Xq12

mRNA and Protein(s)

mRNA	Protein	Name
NM_001135923.1	NP_001129395.1	probable ubiquitin carboxyl-terminal hydrolase FAF-X isoform 2
NM_001135893.1	NP_001129365.1	probable ubiquitin carboxyl-terminal hydrolase FAF-X isoform 1
XM_006256670.3	XP_006256732.1	probable ubiquitin carboxyl-terminal hydrolase FAF-X isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	USP9X	XP_003317476.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010088	XP_319246.4
Homo sapiens (human)	USP9X	NP_001034679.2
Mus musculus (house mouse)	Usp9x	NP_033507.2
Gallus gallus (chicken)	USP9X	XP_004934669.1
Danio rerio (zebrafish)	usp9	NP_001070917.1
Drosophila melanogaster (fruit fly)	faf	NP_733455.1
Bos taurus (cattle)	USP9X	XP_002700252.1
Rattus norvegicus (Norway rat)	Usp9x	NP_001129395.1
Macaca mulatta (Rhesus monkey)	USP9X	XP_002806244.1

Gene Ontology
Bibliography

Related articles in PubMed

Deubiquitinating enzyme USP9X regulates cellular clock function by modulating the ubiquitination and degradation of a core circadian protein BMAL1.
Zhang Y, Duan C, Yang J, Chen S, Liu Q, Zhou L, Huang Z, Xu Y, Xu G
The Biochemical journal475(8)1507-1522(2018 04)

Noncanonical regulation of alkylation damage resistance by the OTUD4 deubiquitinase.
Zhao Y, Majid MC, Soll JM, Brickner JR, Dango S, Mosammaparast N
The EMBO journal34(12)1687-703(2015 Jun)

Mutations in USP9X are associated with X-linked intellectual disability and disrupt neuronal cell migration and growth.
Homan CC, Kumar R, Nguyen LS, Haan E, Raymond FL, Abidi F, Raynaud M, Schwartz CE, Wood SA, Gecz J, Jolly LA
American journal of human genetics94(3)470-8(2014 Mar)

Loss of Usp9x disrupts cortical architecture, hippocampal development and TGFβ-mediated axonogenesis.
Stegeman S, Jolly LA, Premarathne S, Gecz J, Richards LJ, Mackay-Sim A, Wood SA
PloS one8(7)e68287(2013)

Identification of ubiquitin-specific protease 9X (USP9X) as a deubiquitinase acting on ubiquitin-peroxin 5 (PEX5) thioester conjugate.
Grou CP, Francisco T, Rodrigues TA, Freitas MO, Pinto MP, Carvalho AF, Domingues P, Wood SA, Rodríguez-Borges JE, Sá-Miranda C, Fransen M, Azevedo JE
The Journal of biological chemistry287(16)12815-27(2012 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Usp9x gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Usp9x cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa30228	NM_001135923.1 Latest version!	Rattus norvegicus ubiquitin specific peptidase 9, X-linked (Usp9x), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
ORa40415	NM_001135893.1 Latest version!	Rattus norvegicus ubiquitin specific peptidase 9, X-linked (Usp9x), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
ORa30228	XM_006256670.3 Latest version!	Rattus norvegicus ubiquitin specific peptidase 9, X-linked (Usp9x), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Usp9x ( NM_001135923.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001129395.1 Rattus norvegicus ubiquitin specific peptidase 9, X-linked (Usp9x), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ORa30228
Clone ID Related Accession (Same CDS sequence)	NM_001135923.1 , XM_006256670.3
Accession Version	NM_001135923.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7665bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1305388800000
Organism	Rattus norvegicus(Norway rat)
Product	probable ubiquitin carboxyl-terminal hydrolase FAF-X isoform 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AABR03125778.1, AABR03119668.1, AABR03120761.1, AABR03120669.1 and AABR03119288.1. On Oct 17, 2008 this sequence version replaced XM_343766.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

ATGACAGCCA CGACTCGTGG CTCTCCAGTT GGAGGGAATG ACAACCAGGG CCAAGCTCCT 
GATGGACAGT CTCAGCCCCC CCTCCAACAG AATCAGACTT CATCGCCTGA TTCATCCAAT 
GAAAATTCCC CTGCAACTCC TCCTGATGAG CAAGGCCAAG GTGATGCTCC TCCCCAGATT 
GAAGATGAGG AACCTGCATT TCCACACACT GACCTGGCAA AGTTAGATGA TATGATCAAC 
AGGCCTCGCT GGGTCGTTCC AGTTTTGCCG AAAGGGGAAT TAGAAGTGCT TTTAGAAGCT 
GCTATTGATC TTAGTAAGAA AGGCCTTGAT GTCAAAAGTG AAGCATGTCA GAGATTTTTC 
CGAGATGGGC TAACAATCTC ATTCACAAAA ATCCTTACAG ATGAAGCAGT GAGTGGCTGG 
AAGTTTGAAA TTCATAGATG TATTATTAAC AATACTCATC GCCTGGTGGA GCTGTGTGTG 
GCTAAGTTAG CCCAAGACTG GTTTCCACTT CTAGAACTTC TTGCCATGGC CTTAAATCCT 
CATTGCAAAT TCCATATCTA CAATGGTACA CGTCCCTGCG AGTCAGTGTC CTCAAGTGTT 
CAGTTGCCTG AAGATGAACT CTTTGCTCGT TCTCCAGACC CTCGATCACC AAAAGGTTGG 
CTAGTGGATC TCCTCAACAA ATTTGGCACT TTAAATGGAT TCCAGATACT GCATGATCGT 
TTTATTAATG GATCAGCATT AAATGTTCAG ATAATTGCAG CCCTTATTAA ACCATTTGGA 
CAGTGCTATG AGTTTCTTAC TCTTCACACG GTGAAAAAAT ACTTTCTTCC AATAATAGAA 
ATGGTTCCAC AGTTTTTAGA AAACTTAACT GATGAAGAGC TGAAGAAAGA AGCAAAGAAT 
GAAGCCAAAA ATGATGCTCT CTCAATGATT ATTAAATCAT TGAAGAGTTT AGCTTCACGA 
GTTCCTGGAC AGGAAGAAAC TGTAAAGAAC TTAGAAATAT TTAGGTTAAA AATGATACTT 
AGATTATTGC AAATTTCTTC CTTCAATGGA AAGATGAATG CACTGAATGA GGTTAATAAG 
GTGATTTCCA GTGTGTCATA CTATACCCAT CGGCATGGCA GTTCTGAGGA AGAAGAGTGG 
CTCACAGCAG AGAGGATGGC TGAATGGATA CAGCAGAACA ATATTTTATC AATAGTTTTA 
CGAGATAGTC TTCATCAACC ACAGTATGTA GAGAAACTAG AAAAGATTCT TCGCTTTGTC 
ATAAAAGAAA AAGCTCTGAC CTTGCAAGAT CTTGATAATA TCTGGGCAGC ACAGGCAGGG 
AAACATGAAG CCATCGTAAA GAATGTCCAT GATCTACTGG CCAAATTGGC ATGGGATTTT 
TCTCCTGAAC AACTTGATCA TCTTTTTGAT TGCTTTAAGG CCAGTTGGAC AAATGCAAGT 
AAAAAGCAAC GTGAAAAGCT CCTTGAGCTG ATTCGTCGTC TTGCAGAAGA TGATAAAGAT 
GGTGTGATGG CACATAAAGT GTTGAACCTT CTGTGGAATC TAGCTCACAG TGATGATGTG 
CCTGTGGATA TCATGGACTT GGCTCTCAGT GCTCACATCA AAATACTAGA TTATAGTTGC 
TCCCAGGACC GGGACACACA AAAGATCCAG TGGATAGATC GCTTCATAGA AGAACTTCGC 
ACAAATGACA AGTGGGTCAT TCCTGCACTG AAACAAATTA GAGAAATTTG CAGTTTGTTT 
GGTGAAGCAC CTCAAAATTT GAGTCAAACT CAGAGAAGTC CCCATGTATT TTATCGCCAT 
GATTTAATCA ATCAACTTCA GCACAATCAT GCCCTAGTTA CTTTGGTAGC AGAAAACCTT 
GCAACTTACA TGGAAAGCAT GAGACTGTAT GGCAGAGACA ATGAAGACTA TGACCCACAA 
ACTGTGAGAG TGGGAAGTAG ATATAGTCAT GTTCAAGAAG TCCAAGAACG TCTTAACTTT 
CTTAGATTTT TATTGAAGGA TGGCCAGCTA TGGCTGTGTG CTCCTCAGGC AAAACAAATA 
TGGAAGTGCT TAGCAGAGAA TGCGGTTTAT CTTTGTGACC GTGAAGCCTG CTTTAAGTGG 
TATTCCAAAT TAATGGGAGA TGAACCAGAC TTAGATCCTG ATATCAATAA GGACTTCTTT 
GAAAGTAATG TGCTTCAGCT TGATCCTTCA CTATTAACTG AAAATGGAAT GAAATGTTTT 
GAGAGATTCT TCAAAGCTGT GAATTGTCGA GAAGGAAAAC TAGTAGCAAA AAGAAGAGCC 
TATATGATGG ATGATTTGGA ATTGATAGGA TTAGACTATC TTTGGAGGGT TGTAATTCAG 
AGTAATGATG ATATTGCCAG CCGAGCTATT GATCTCCTCA AAGAGATATA CACAAACCTT 
GGTCCAAGAC TGCAGGTCAA TCAGGTGGTG ATCCATGAAG ACTTCATTCA GTCTTGCTTT 
GATCGTTTGA AAGCCTCTTA TGACACGTTG TGTGTTTTGG ATGGTGACAA AGACAGTATT 
AATTGTGCAA GACAGGAAGC TGTTCGAATG GTCCGAGTAT TAACTGTTTT AAGAGAATAT 
ATAAATGAAT GTGACAGTGA TTATCATGAA GAAAGAACAA TTTTGCCTAT GTCAAGAGCT 
TTCCGTGGTA AACACCTCTC TTTTATAGTT CGGTTTCCAA ACCAGGGCAG ACAAGTAGAT 
GACTTGGAAG TTTGGTCTCA TACAAATGAT ACCATTGGTT CAGTTCGACG ATGTATACTA 
AATCGCATTA AAGCCAATGT AGCTCATACA AAAATTGAAC TCTTTGTGGG TGGTGAGCTT 
ATTGATCCTG GAGATGACAG AAAGTTGATT GGACAATTAA ACCTAAAAGA TAAATCACTA 
ATTACAGCCA AACTTACACA AATCAGCTCC AATATGCCGT CAAGTCCTGA TAGCTCCTCT 
GATTCCTCAA CTGGATCTCC TGGAAACCAT GGTAATCATT ACAGTGATGG TCCCAACCCA 
GAGGTAGAAA GCTGTTTGCC TGGAGTGATA ATGTCACTTC ATCCCAGATA CATCTCTTTC 
CTTTGGCAAG TTGCAGACTT AGGGAGCAGC CTAAATATGC CCCCTCTTAG AGATGGAGCA 
AGAGTTCTTA TGAAACTTAT GCCGCCAGAT AGTACAACAA TAGAAAAATT AAGAGCTATT 
TGTTTAGATC ATGCCAAACT TGGAGAAAGC AGCCTTAGTC CATCTCTTGA CTCACTTTTC 
TTTGGTCCTT CAGCCTCACA AGTGCTATAC CTAACAGAGG TAGTCTATGC CCTGTTAATG 
CCTGCTGGTG CACCCCTGGC TGATGACTCC TCTGATTTTC AGTTTCACTT CTTGAAAAGT 
GGTGGTTTGC CCCTTGTTCT GAGTATGCTA ACCAGAAATA ACTTCCTACC AAATGCTGAT 
ATGGAAACTC GAAGGGGTGC CTACCTCAAT GCTCTTAAAA TAGCCAAACT GTTACTAACT 
GCCATTGGCT ATGGCCATGT TCGGGCTGTG GCAGAAGCAT GTCAGCCAGG TGTAGAAGGC 
GTGAATCCTA TGACATCGGT CAACCAAGTA ACTCATGATC AAGCAGTGGT GCTACAAAGT 
GCCCTTCAGA GCATTCCTAA CCCATCATCT GAATGCATGC TTAGAAACGT GTCTGTTCGT 
CTTGCTCAGC AGATTTCTGA TGAGGCTTCA AGATATATGC CTGATATTTG TGTAATTAGA 
GCTATACAAA AGATTATTTG GACATCAGGA TGTGGGGGAT TACAACTGGT ATTCAGCCCA 
AATGAGGAAG TCACAAAAAT TTATGAGAAG ACCAATGCAG GCAATGAGCC AGACCTTGAA 
GATGAACAGG TTTGCTGTGA GGCATTGGAA GTGATGACAC TGTGTTTCGC CCTGATTCCA 
ACAGCCTTAG ATGCTCTAAG TAAAGAAAAG GCTTGGCAGA CATTTATTAT TGACTTACTG 
TTACACTGTC ACAGCAAAAC TGTCCGTCAA GTAGCACAGG AACAATTCTT CTTAATGTGC 
ACCAGATGTT GCATGGGACA CAGGCCTCTA CTTTTCTTCA TTACTCTACT CTTTACTGTT 
TTGGGGAGCA CAGCCAGAGA GAGGGCTAAA CATTCAGGCG ACTACTTTAC TCTTTTAAGG 
CATCTTCTTA ATTACGCTTA CAACAGTAAC ATTAATGTAC CCAATGCTGA AGTTCTTCTA 
AATAATGAAA TTGATTGGCT TAAAAGAATT AGGGATGATG TTAAAAGAAC AGGTGAAACA 
GGCGTTGAAG AGACAATCTT AGAAGGACAC CTTGGGGTTA CAAAGGAGCT ACTGGCTTTT 
CAGACCCCTG AGAAAAAGTT TCATATTGGT TGTGAAAAGG GGGGTGCTAA TCTCATTAAA 
GAATTAATCG ACGATTTCAT CTTTCCTGCA TCCAATGTTT ACCTCCAGTA TATGAGAAAT 
GGAGAACTCC CTGCTGAGCA GGCCATTCCT GTCTGTGGTT CACCAGCCAC AATTAATGCT 
GGTTTTGAGT TACTTGTAGC ATTAGCTGTT GGCTGTGTAA GGAACCTCAA ACAGATAGTA 
GATTCTTTGA CTGAAATGTA TTACATTGGT ACAGCAATAA CTACTTGTGA GGCACTTACT 
GAGTGGGAAT ACCTGCCACC TGTTGGACCC CGTCCACCAA AAGGATTTGT GGGGCTGAAA 
AATGCTGGTG CTACTTGTTA CATGAATTCT GTGATTCAGC AACTCTATAT GATTCCCTCT 
ATCAGGAACG GTATTCTTGC AATTGAAGGC ACAGGTAGTG ATGTAGATGA TGATATGTCT 
GGGGATGAGA AGCAGGACAA TGAGAGCAAT GTTGATCCCA GGGATGATGT GTTTGGATAT 
CCTCAACAAT TTGAAGACAA ACCACCACTG AGTAAAACAG AAGATAGAAA AGAGTACAAT 
ATTGGTGTTC TAAGACACCT TCAAGTTATC TTTGGCCATT TAGCTGCTTC TCGACTACAG 
TACTATGTGC CCAGAGGATT TTGGAAACAG TTCAGGCTTT GGGGTGAGCC TGTTAATCTT 
CGTGAACAAC ATGATGCTTT AGAGTTCTTT AATTCATTGG TGGATAGTTT AGATGAAGCT 
TTAAAAGCCT TAGGGCATCC AGCTATGCTA AGTAAAGTCT TGGGAGGTTC CTTCGCTGAT 
CAGAAAATTT GTCAAGGCTG CCCACATAGG TATGAATGTG AAGAATCTTT TACGACTCTG 
AATGTAGACA TTAGAAACCA CCAAAATCTT CTTGATTCTT TGGAACAGTA TGTCAAAGGA 
GATTTACTAG AAGGGGCAAA TGCTTATCAT TGTGAAAAAT GCAATAAAAA GGTTGATACT 
GTAAAGCGCT TGCTAATTAA AAAGTTGCCT CCTGTTCTTG CTATCCAACT AAAACGATTC 
GATTATGACT GGGAAAGAGA ATGTGCAATC AAGTTCAATG ATTACTTTGA GTTTCCTCGA 
GAGTTAGACA TGGAGCCGTA CACAGTTGCA GGTGTTGCTA AACTAGAGGG AGATAATGTC 
AACCCAGAAA GTCAGCTGAT ACAACAGAAT GAGCAGTCTG AAAGCGAAAA AGCCGGAAGC 
ACAAAATACA GACTTGTGGG TGTGCTTGTA CACAGTGGTC AAGCAAGTGG GGGACATTAC 
TATTCTTACA TCATTCAGAG GAATGGAGGA GATGGTGAAA AAAATCGTTG GTATAAATTT 
GATGATGGAG ATGTAACAGA GTGTAAAATG GATGATGATG AAGAAATGAA AAACCAGTGT 
TTTGGTGGCG AGTACATGGG AGAAGTGTTT GATCACATGA TGAAGCGCAT GTCGTACAGG 
CGGCAGAAAA GGTGGTGGAA TGCATATATA CTTTTTTATG AAAGAATGGA TACGATAGAT 
CATGATGATG AGGTGATAAG ATACATATCA GAGATTGCTA TCACCACAAG GCCCCATCAG 
ATTGTTATGC CATCGGCCAT TGAAAGAAGT GTGCGGAAGC AGAATGTCCA ATTCATGCAT 
AACCGAATGC AGTACAGTTT GGAATATTTT CAGTTTATGA AAAAGCTACT TACATGTAAT 
GGTGTTTATT TAAACCCTCC ACCAGGGCAA GATCACCTGT CTCCTGAAGC AGAAGAAATC 
ACTATGATTA GTATTCAGCT TGCTGCTAGG TTCCTTTTTA CTACAGGATT TCACACAAAG 
AAAATAGTCC GAGGATCCGC CAGTGATTGG TATGATGCAT TGTGTATTCT CCTCCGTCAC 
AGCAAGAATG TGCGGTTTTG GTTTGCTCAC AATGTGCTTT TTAATGTTTC AAATCGATTC 
TCTGAGTACC TTTTGGAATG CCCTAGTGCA GAAGTGAGAG GTGCATTTGC TAAACTTATA 
GTTTTCATTG CACACTTTTC CTTACAAGAT GGGCCTTGTC CTTCACCTTT TGCATCTCCT 
GGACCTTCTA GTCAGGCTTA TGATAACTTA AGTTTGAGTG ACCACCTACT AAGAGCAGTA 
CTAAATCTCT TGAGGAGGGA GGTTTCAGAG CATGGACGTC ATTTACAGCA GTATTTCAAC 
TTGTTTGTAA TGTATGCCAA TTTAGGAGTG GCAGAGAAAA CACAGCTGCT CAAACTGAGT 
GTACCTGCTA CCTTTATGCT TGTGTCCTTA GATGAAGGTC CTGGTCCTCC AATTAAATAT 
CAGTATGCTG AATTAGGCAA ATTATACTCC GTAGTGTCAC AGTTAATCCG CTGTTGCAAT 
GTCTCTTCAA GAATGCAGTC TTCAATCAAT GGTAATCCTT CTCTTCCAAA TCCTTTTGGT 
GATCCTAATT TATCACAACC TATAATGCCA ATTCAACAAA ATGTGGTAGA CATTTTATTT 
GTGAGAACAA GTTATGTGAA GAAAATTATT GAAGACTGCA GTAACTCTGA TGAGACCGTC 
AAATTGCTTC GCTTTTGCTG CTGGGAGAAT CCTCAGTTCT CATCTACTGT CCTGAGTGAA 
CTTCTTTGGC AGGTTGCGTA TTCCTATACT TACGAACTCC GGCCCTATTT GGATCTGCTT 
TTACAAATCT TACTGATTGA AGACTCCTGG CAGACTCACA GAATTCATAA TGCCCTTAAA 
GGAATTCCAG ATGACCGAGA TGGGCTGTTT GACACAATTC AGCGTTCTAA GAATCACTAT 
CAAAAAAGAG CATACCAATG TATAAAATGT ATGGTAGCCC TCTTTAGCAG TTGTCCTGTG 
GCTTACCAAA TCCTGCAGGG CAATGGAGAT CTTAAAAGGA AATGGACCTG GGCAGTAGAG 
TGGCTTGGAG ATGAACTCGA AAGAAGACCA TACACTGGCA ATCCTCAGTA CACTTACAAC 
AACTGGTCTC CTCCAGTGCA AAGCAATGAA ACATCAAATG GCTACTTCTT GGAGAGATCA 
CATAGCGCTA GGATGACACT TGCAAAAGCT TGTGAACTCT GTCCTGAGGA GGAACCAGAT 
GACCAAGATG CCCCAGATGA GCATGAATCA CCTCCACCTG AAGATGCCCC ATTATACCCC 
CATTCCCCTG GATCTCAATA TCAGCAGAAT AACCATGTGC ATGGACAGCC CTATACAGGC 
CCAGCCGCAC ATCACATGAA CAACCCTCAG AGAACTGGCC AACGAGCACA AGAAAATTAT 
GAAGGCAGTG AAGAAGTGTC CCCACCTCAG ACCAAGGATC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001129395.1
CDS	373..8037
Translation	MTATTRGSPVGGNDNQGQAPDGQSQPPLQQNQTSSPDSSNENSPATPPDEQGQGDAPPQIEDEEPAFPHTDLAKLDDMINRPRWVVPVLPKGELEVLLEAAIDLSKKGLDVKSEACQRFFRDGLTISFTKILTDEAVSGWKFEIHRCIINNTHRLVELCVAKLAQDWFPLLELLAMALNPHCKFHIYNGTRPCESVSSSVQLPEDELFARSPDPRSPKGWLVDLLNKFGTLNGFQILHDRFINGSALNVQIIAALIKPFGQCYEFLTLHTVKKYFLPIIEMVPQFLENLTDEELKKEAKNEAKNDALSMIIKSLKSLASRVPGQEETVKNLEIFRLKMILRLLQISSFNGKMNALNEVNKVISSVSYYTHRHGSSEEEEWLTAERMAEWIQQNNILSIVLRDSLHQPQYVEKLEKILRFVIKEKALTLQDLDNIWAAQAGKHEAIVKNVHDLLAKLAWDFSPEQLDHLFDCFKASWTNASKKQREKLLELIRRLAEDDKDGVMAHKVLNLLWNLAHSDDVPVDIMDLALSAHIKILDYSCSQDRDTQKIQWIDRFIEELRTNDKWVIPALKQIREICSLFGEAPQNLSQTQRSPHVFYRHDLINQLQHNHALVTLVAENLATYMESMRLYGRDNEDYDPQTVRVGSRYSHVQEVQERLNFLRFLLKDGQLWLCAPQAKQIWKCLAENAVYLCDREACFKWYSKLMGDEPDLDPDINKDFFESNVLQLDPSLLTENGMKCFERFFKAVNCREGKLVAKRRAYMMDDLELIGLDYLWRVVIQSNDDIASRAIDLLKEIYTNLGPRLQVNQVVIHEDFIQSCFDRLKASYDTLCVLDGDKDSINCARQEAVRMVRVLTVLREYINECDSDYHEERTILPMSRAFRGKHLSFIVRFPNQGRQVDDLEVWSHTNDTIGSVRRCILNRIKANVAHTKIELFVGGELIDPGDDRKLIGQLNLKDKSLITAKLTQISSNMPSSPDSSSDSSTGSPGNHGNHYSDGPNPEVESCLPGVIMSLHPRYISFLWQVADLGSSLNMPPLRDGARVLMKLMPPDSTTIEKLRAICLDHAKLGESSLSPSLDSLFFGPSASQVLYLTEVVYALLMPAGAPLADDSSDFQFHFLKSGGLPLVLSMLTRNNFLPNADMETRRGAYLNALKIAKLLLTAIGYGHVRAVAEACQPGVEGVNPMTSVNQVTHDQAVVLQSALQSIPNPSSECMLRNVSVRLAQQISDEASRYMPDICVIRAIQKIIWTSGCGGLQLVFSPNEEVTKIYEKTNAGNEPDLEDEQVCCEALEVMTLCFALIPTALDALSKEKAWQTFIIDLLLHCHSKTVRQVAQEQFFLMCTRCCMGHRPLLFFITLLFTVLGSTARERAKHSGDYFTLLRHLLNYAYNSNINVPNAEVLLNNEIDWLKRIRDDVKRTGETGVEETILEGHLGVTKELLAFQTPEKKFHIGCEKGGANLIKELIDDFIFPASNVYLQYMRNGELPAEQAIPVCGSPATINAGFELLVALAVGCVRNLKQIVDSLTEMYYIGTAITTCEALTEWEYLPPVGPRPPKGFVGLKNAGATCYMNSVIQQLYMIPSIRNGILAIEGTGSDVDDDMSGDEKQDNESNVDPRDDVFGYPQQFEDKPPLSKTEDRKEYNIGVLRHLQVIFGHLAASRLQYYVPRGFWKQFRLWGEPVNLREQHDALEFFNSLVDSLDEALKALGHPAMLSKVLGGSFADQKICQGCPHRYECEESFTTLNVDIRNHQNLLDSLEQYVKGDLLEGANAYHCEKCNKKVDTVKRLLIKKLPPVLAIQLKRFDYDWERECAIKFNDYFEFPRELDMEPYTVAGVAKLEGDNVNPESQLIQQNEQSESEKAGSTKYRLVGVLVHSGQASGGHYYSYIIQRNGGDGEKNRWYKFDDGDVTECKMDDDEEMKNQCFGGEYMGEVFDHMMKRMSYRRQKRWWNAYILFYERMDTIDHDDEVIRYISEIAITTRPHQIVMPSAIERSVRKQNVQFMHNRMQYSLEYFQFMKKLLTCNGVYLNPPPGQDHLSPEAEEITMISIQLAARFLFTTGFHTKKIVRGSASDWYDALCILLRHSKNVRFWFAHNVLFNVSNRFSEYLLECPSAEVRGAFAKLIVFIAHFSLQDGPCPSPFASPGPSSQAYDNLSLSDHLLRAVLNLLRREVSEHGRHLQQYFNLFVMYANLGVAEKTQLLKLSVPATFMLVSLDEGPGPPIKYQYAELGKLYSVVSQLIRCCNVSSRMQSSINGNPSLPNPFGDPNLSQPIMPIQQNVVDILFVRTSYVKKIIEDCSNSDETVKLLRFCCWENPQFSSTVLSELLWQVAYSYTYELRPYLDLLLQILLIEDSWQTHRIHNALKGIPDDRDGLFDTIQRSKNHYQKRAYQCIKCMVALFSSCPVAYQILQGNGDLKRKWTWAVEWLGDELERRPYTGNPQYTYNNWSPPVQSNETSNGYFLERSHSARMTLAKACELCPEEEPDDQDAPDEHESPPPEDAPLYPHSPGSQYQQNNHVHGQPYTGPAAHHMNNPQRTGQRAQENYEGSEEVSPPQTKDQ

Target ORF information:

RefSeq Version	NM_001135923.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ubiquitin specific peptidase 9, X-linked (Usp9x), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001135923.1

ORF Insert Sequence:

ATGACAGCCA CGACTCGTGG CTCTCCAGTT GGAGGGAATG ACAACCAGGG CCAAGCTCCT 
GATGGACAGT CTCAGCCCCC CCTCCAACAG AATCAGACTT CATCGCCTGA TTCATCCAAT 
GAAAATTCCC CTGCAACTCC TCCTGATGAG CAAGGCCAAG GTGATGCTCC TCCCCAGATT 
GAAGATGAGG AACCTGCATT TCCACACACT GACCTGGCAA AGTTAGATGA TATGATCAAC 
AGGCCTCGCT GGGTCGTTCC AGTTTTGCCG AAAGGGGAAT TAGAAGTGCT TTTAGAAGCT 
GCTATTGATC TTAGTAAGAA AGGCCTTGAT GTCAAAAGTG AAGCATGTCA GAGATTTTTC 
CGAGATGGGC TAACAATCTC ATTCACAAAA ATCCTTACAG ATGAAGCAGT GAGTGGCTGG 
AAGTTTGAAA TTCATAGATG TATTATTAAC AATACTCATC GCCTGGTGGA GCTGTGTGTG 
GCTAAGTTAG CCCAAGACTG GTTTCCACTT CTAGAACTTC TTGCCATGGC CTTAAATCCT 
CATTGCAAAT TCCATATCTA CAATGGTACA CGTCCCTGCG AGTCAGTGTC CTCAAGTGTT 
CAGTTGCCTG AAGATGAACT CTTTGCTCGT TCTCCAGACC CTCGATCACC AAAAGGTTGG 
CTAGTGGATC TCCTCAACAA ATTTGGCACT TTAAATGGAT TCCAGATACT GCATGATCGT 
TTTATTAATG GATCAGCATT AAATGTTCAG ATAATTGCAG CCCTTATTAA ACCATTTGGA 
CAGTGCTATG AGTTTCTTAC TCTTCACACG GTGAAAAAAT ACTTTCTTCC AATAATAGAA 
ATGGTTCCAC AGTTTTTAGA AAACTTAACT GATGAAGAGC TGAAGAAAGA AGCAAAGAAT 
GAAGCCAAAA ATGATGCTCT CTCAATGATT ATTAAATCAT TGAAGAGTTT AGCTTCACGA 
GTTCCTGGAC AGGAAGAAAC TGTAAAGAAC TTAGAAATAT TTAGGTTAAA AATGATACTT 
AGATTATTGC AAATTTCTTC CTTCAATGGA AAGATGAATG CACTGAATGA GGTTAATAAG 
GTGATTTCCA GTGTGTCATA CTATACCCAT CGGCATGGCA GTTCTGAGGA AGAAGAGTGG 
CTCACAGCAG AGAGGATGGC TGAATGGATA CAGCAGAACA ATATTTTATC AATAGTTTTA 
CGAGATAGTC TTCATCAACC ACAGTATGTA GAGAAACTAG AAAAGATTCT TCGCTTTGTC 
ATAAAAGAAA AAGCTCTGAC CTTGCAAGAT CTTGATAATA TCTGGGCAGC ACAGGCAGGG 
AAACATGAAG CCATCGTAAA GAATGTCCAT GATCTACTGG CCAAATTGGC ATGGGATTTT 
TCTCCTGAAC AACTTGATCA TCTTTTTGAT TGCTTTAAGG CCAGTTGGAC AAATGCAAGT 
AAAAAGCAAC GTGAAAAGCT CCTTGAGCTG ATTCGTCGTC TTGCAGAAGA TGATAAAGAT 
GGTGTGATGG CACATAAAGT GTTGAACCTT CTGTGGAATC TAGCTCACAG TGATGATGTG 
CCTGTGGATA TCATGGACTT GGCTCTCAGT GCTCACATCA AAATACTAGA TTATAGTTGC 
TCCCAGGACC GGGACACACA AAAGATCCAG TGGATAGATC GCTTCATAGA AGAACTTCGC 
ACAAATGACA AGTGGGTCAT TCCTGCACTG AAACAAATTA GAGAAATTTG CAGTTTGTTT 
GGTGAAGCAC CTCAAAATTT GAGTCAAACT CAGAGAAGTC CCCATGTATT TTATCGCCAT 
GATTTAATCA ATCAACTTCA GCACAATCAT GCCCTAGTTA CTTTGGTAGC AGAAAACCTT 
GCAACTTACA TGGAAAGCAT GAGACTGTAT GGCAGAGACA ATGAAGACTA TGACCCACAA 
ACTGTGAGAG TGGGAAGTAG ATATAGTCAT GTTCAAGAAG TCCAAGAACG TCTTAACTTT 
CTTAGATTTT TATTGAAGGA TGGCCAGCTA TGGCTGTGTG CTCCTCAGGC AAAACAAATA 
TGGAAGTGCT TAGCAGAGAA TGCGGTTTAT CTTTGTGACC GTGAAGCCTG CTTTAAGTGG 
TATTCCAAAT TAATGGGAGA TGAACCAGAC TTAGATCCTG ATATCAATAA GGACTTCTTT 
GAAAGTAATG TGCTTCAGCT TGATCCTTCA CTATTAACTG AAAATGGAAT GAAATGTTTT 
GAGAGATTCT TCAAAGCTGT GAATTGTCGA GAAGGAAAAC TAGTAGCAAA AAGAAGAGCC 
TATATGATGG ATGATTTGGA ATTGATAGGA TTAGACTATC TTTGGAGGGT TGTAATTCAG 
AGTAATGATG ATATTGCCAG CCGAGCTATT GATCTCCTCA AAGAGATATA CACAAACCTT 
GGTCCAAGAC TGCAGGTCAA TCAGGTGGTG ATCCATGAAG ACTTCATTCA GTCTTGCTTT 
GATCGTTTGA AAGCCTCTTA TGACACGTTG TGTGTTTTGG ATGGTGACAA AGACAGTATT 
AATTGTGCAA GACAGGAAGC TGTTCGAATG GTCCGAGTAT TAACTGTTTT AAGAGAATAT 
ATAAATGAAT GTGACAGTGA TTATCATGAA GAAAGAACAA TTTTGCCTAT GTCAAGAGCT 
TTCCGTGGTA AACACCTCTC TTTTATAGTT CGGTTTCCAA ACCAGGGCAG ACAAGTAGAT 
GACTTGGAAG TTTGGTCTCA TACAAATGAT ACCATTGGTT CAGTTCGACG ATGTATACTA 
AATCGCATTA AAGCCAATGT AGCTCATACA AAAATTGAAC TCTTTGTGGG TGGTGAGCTT 
ATTGATCCTG GAGATGACAG AAAGTTGATT GGACAATTAA ACCTAAAAGA TAAATCACTA 
ATTACAGCCA AACTTACACA AATCAGCTCC AATATGCCGT CAAGTCCTGA TAGCTCCTCT 
GATTCCTCAA CTGGATCTCC TGGAAACCAT GGTAATCATT ACAGTGATGG TCCCAACCCA 
GAGGTAGAAA GCTGTTTGCC TGGAGTGATA ATGTCACTTC ATCCCAGATA CATCTCTTTC 
CTTTGGCAAG TTGCAGACTT AGGGAGCAGC CTAAATATGC CCCCTCTTAG AGATGGAGCA 
AGAGTTCTTA TGAAACTTAT GCCGCCAGAT AGTACAACAA TAGAAAAATT AAGAGCTATT 
TGTTTAGATC ATGCCAAACT TGGAGAAAGC AGCCTTAGTC CATCTCTTGA CTCACTTTTC 
TTTGGTCCTT CAGCCTCACA AGTGCTATAC CTAACAGAGG TAGTCTATGC CCTGTTAATG 
CCTGCTGGTG CACCCCTGGC TGATGACTCC TCTGATTTTC AGTTTCACTT CTTGAAAAGT 
GGTGGTTTGC CCCTTGTTCT GAGTATGCTA ACCAGAAATA ACTTCCTACC AAATGCTGAT 
ATGGAAACTC GAAGGGGTGC CTACCTCAAT GCTCTTAAAA TAGCCAAACT GTTACTAACT 
GCCATTGGCT ATGGCCATGT TCGGGCTGTG GCAGAAGCAT GTCAGCCAGG TGTAGAAGGC 
GTGAATCCTA TGACATCGGT CAACCAAGTA ACTCATGATC AAGCAGTGGT GCTACAAAGT 
GCCCTTCAGA GCATTCCTAA CCCATCATCT GAATGCATGC TTAGAAACGT GTCTGTTCGT 
CTTGCTCAGC AGATTTCTGA TGAGGCTTCA AGATATATGC CTGATATTTG TGTAATTAGA 
GCTATACAAA AGATTATTTG GACATCAGGA TGTGGGGGAT TACAACTGGT ATTCAGCCCA 
AATGAGGAAG TCACAAAAAT TTATGAGAAG ACCAATGCAG GCAATGAGCC AGACCTTGAA 
GATGAACAGG TTTGCTGTGA GGCATTGGAA GTGATGACAC TGTGTTTCGC CCTGATTCCA 
ACAGCCTTAG ATGCTCTAAG TAAAGAAAAG GCTTGGCAGA CATTTATTAT TGACTTACTG 
TTACACTGTC ACAGCAAAAC TGTCCGTCAA GTAGCACAGG AACAATTCTT CTTAATGTGC 
ACCAGATGTT GCATGGGACA CAGGCCTCTA CTTTTCTTCA TTACTCTACT CTTTACTGTT 
TTGGGGAGCA CAGCCAGAGA GAGGGCTAAA CATTCAGGCG ACTACTTTAC TCTTTTAAGG 
CATCTTCTTA ATTACGCTTA CAACAGTAAC ATTAATGTAC CCAATGCTGA AGTTCTTCTA 
AATAATGAAA TTGATTGGCT TAAAAGAATT AGGGATGATG TTAAAAGAAC AGGTGAAACA 
GGCGTTGAAG AGACAATCTT AGAAGGACAC CTTGGGGTTA CAAAGGAGCT ACTGGCTTTT 
CAGACCCCTG AGAAAAAGTT TCATATTGGT TGTGAAAAGG GGGGTGCTAA TCTCATTAAA 
GAATTAATCG ACGATTTCAT CTTTCCTGCA TCCAATGTTT ACCTCCAGTA TATGAGAAAT 
GGAGAACTCC CTGCTGAGCA GGCCATTCCT GTCTGTGGTT CACCAGCCAC AATTAATGCT 
GGTTTTGAGT TACTTGTAGC ATTAGCTGTT GGCTGTGTAA GGAACCTCAA ACAGATAGTA 
GATTCTTTGA CTGAAATGTA TTACATTGGT ACAGCAATAA CTACTTGTGA GGCACTTACT 
GAGTGGGAAT ACCTGCCACC TGTTGGACCC CGTCCACCAA AAGGATTTGT GGGGCTGAAA 
AATGCTGGTG CTACTTGTTA CATGAATTCT GTGATTCAGC AACTCTATAT GATTCCCTCT 
ATCAGGAACG GTATTCTTGC AATTGAAGGC ACAGGTAGTG ATGTAGATGA TGATATGTCT 
GGGGATGAGA AGCAGGACAA TGAGAGCAAT GTTGATCCCA GGGATGATGT GTTTGGATAT 
CCTCAACAAT TTGAAGACAA ACCACCACTG AGTAAAACAG AAGATAGAAA AGAGTACAAT 
ATTGGTGTTC TAAGACACCT TCAAGTTATC TTTGGCCATT TAGCTGCTTC TCGACTACAG 
TACTATGTGC CCAGAGGATT TTGGAAACAG TTCAGGCTTT GGGGTGAGCC TGTTAATCTT 
CGTGAACAAC ATGATGCTTT AGAGTTCTTT AATTCATTGG TGGATAGTTT AGATGAAGCT 
TTAAAAGCCT TAGGGCATCC AGCTATGCTA AGTAAAGTCT TGGGAGGTTC CTTCGCTGAT 
CAGAAAATTT GTCAAGGCTG CCCACATAGG TATGAATGTG AAGAATCTTT TACGACTCTG 
AATGTAGACA TTAGAAACCA CCAAAATCTT CTTGATTCTT TGGAACAGTA TGTCAAAGGA 
GATTTACTAG AAGGGGCAAA TGCTTATCAT TGTGAAAAAT GCAATAAAAA GGTTGATACT 
GTAAAGCGCT TGCTAATTAA AAAGTTGCCT CCTGTTCTTG CTATCCAACT AAAACGATTC 
GATTATGACT GGGAAAGAGA ATGTGCAATC AAGTTCAATG ATTACTTTGA GTTTCCTCGA 
GAGTTAGACA TGGAGCCGTA CACAGTTGCA GGTGTTGCTA AACTAGAGGG AGATAATGTC 
AACCCAGAAA GTCAGCTGAT ACAACAGAAT GAGCAGTCTG AAAGCGAAAA AGCCGGAAGC 
ACAAAATACA GACTTGTGGG TGTGCTTGTA CACAGTGGTC AAGCAAGTGG GGGACATTAC 
TATTCTTACA TCATTCAGAG GAATGGAGGA GATGGTGAAA AAAATCGTTG GTATAAATTT 
GATGATGGAG ATGTAACAGA GTGTAAAATG GATGATGATG AAGAAATGAA AAACCAGTGT 
TTTGGTGGCG AGTACATGGG AGAAGTGTTT GATCACATGA TGAAGCGCAT GTCGTACAGG 
CGGCAGAAAA GGTGGTGGAA TGCATATATA CTTTTTTATG AAAGAATGGA TACGATAGAT 
CATGATGATG AGGTGATAAG ATACATATCA GAGATTGCTA TCACCACAAG GCCCCATCAG 
ATTGTTATGC CATCGGCCAT TGAAAGAAGT GTGCGGAAGC AGAATGTCCA ATTCATGCAT 
AACCGAATGC AGTACAGTTT GGAATATTTT CAGTTTATGA AAAAGCTACT TACATGTAAT 
GGTGTTTATT TAAACCCTCC ACCAGGGCAA GATCACCTGT CTCCTGAAGC AGAAGAAATC 
ACTATGATTA GTATTCAGCT TGCTGCTAGG TTCCTTTTTA CTACAGGATT TCACACAAAG 
AAAATAGTCC GAGGATCCGC CAGTGATTGG TATGATGCAT TGTGTATTCT CCTCCGTCAC 
AGCAAGAATG TGCGGTTTTG GTTTGCTCAC AATGTGCTTT TTAATGTTTC AAATCGATTC 
TCTGAGTACC TTTTGGAATG CCCTAGTGCA GAAGTGAGAG GTGCATTTGC TAAACTTATA 
GTTTTCATTG CACACTTTTC CTTACAAGAT GGGCCTTGTC CTTCACCTTT TGCATCTCCT 
GGACCTTCTA GTCAGGCTTA TGATAACTTA AGTTTGAGTG ACCACCTACT AAGAGCAGTA 
CTAAATCTCT TGAGGAGGGA GGTTTCAGAG CATGGACGTC ATTTACAGCA GTATTTCAAC 
TTGTTTGTAA TGTATGCCAA TTTAGGAGTG GCAGAGAAAA CACAGCTGCT CAAACTGAGT 
GTACCTGCTA CCTTTATGCT TGTGTCCTTA GATGAAGGTC CTGGTCCTCC AATTAAATAT 
CAGTATGCTG AATTAGGCAA ATTATACTCC GTAGTGTCAC AGTTAATCCG CTGTTGCAAT 
GTCTCTTCAA GAATGCAGTC TTCAATCAAT GGTAATCCTT CTCTTCCAAA TCCTTTTGGT 
GATCCTAATT TATCACAACC TATAATGCCA ATTCAACAAA ATGTGGTAGA CATTTTATTT 
GTGAGAACAA GTTATGTGAA GAAAATTATT GAAGACTGCA GTAACTCTGA TGAGACCGTC 
AAATTGCTTC GCTTTTGCTG CTGGGAGAAT CCTCAGTTCT CATCTACTGT CCTGAGTGAA 
CTTCTTTGGC AGGTTGCGTA TTCCTATACT TACGAACTCC GGCCCTATTT GGATCTGCTT 
TTACAAATCT TACTGATTGA AGACTCCTGG CAGACTCACA GAATTCATAA TGCCCTTAAA 
GGAATTCCAG ATGACCGAGA TGGGCTGTTT GACACAATTC AGCGTTCTAA GAATCACTAT 
CAAAAAAGAG CATACCAATG TATAAAATGT ATGGTAGCCC TCTTTAGCAG TTGTCCTGTG 
GCTTACCAAA TCCTGCAGGG CAATGGAGAT CTTAAAAGGA AATGGACCTG GGCAGTAGAG 
TGGCTTGGAG ATGAACTCGA AAGAAGACCA TACACTGGCA ATCCTCAGTA CACTTACAAC 
AACTGGTCTC CTCCAGTGCA AAGCAATGAA ACATCAAATG GCTACTTCTT GGAGAGATCA 
CATAGCGCTA GGATGACACT TGCAAAAGCT TGTGAACTCT GTCCTGAGGA GGAACCAGAT 
GACCAAGATG CCCCAGATGA GCATGAATCA CCTCCACCTG AAGATGCCCC ATTATACCCC 
CATTCCCCTG GATCTCAATA TCAGCAGAAT AACCATGTGC ATGGACAGCC CTATACAGGC 
CCAGCCGCAC ATCACATGAA CAACCCTCAG AGAACTGGCC AACGAGCACA AGAAAATTAT 
GAAGGCAGTG AAGAAGTGTC CCCACCTCAG ACCAAGGATC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usp9x ( NM_001135893.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001129365.1 Rattus norvegicus ubiquitin specific peptidase 9, X-linked (Usp9x), transcript variant 1, mRNA.

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CloneID	ORa40415
Clone ID Related Accession (Same CDS sequence)	NM_001135893.1
Accession Version	NM_001135893.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7644bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1555516800000
Organism	Rattus norvegicus(Norway rat)
Product	probable ubiquitin carboxyl-terminal hydrolase FAF-X isoform 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH474009.2. On Oct 21, 2008 this sequence version replaced XM_001056701.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMEA2689594 [ECO:0000350] ##Evidence-Data-END##

ATGACAGCCA CGACTCGTGG CTCTCCAGTT GGAGGGAATG ACAACCAGGG CCAAGCTCCT 
GATGGACAGT CTCAGCCCCC CCTCCAACAG AATCAGACTT CATCGCCTGA TTCATCCAAT 
GAAAATTCCC CTGCAACTCC TCCTGATGAG CAAGGCCAAG GTGATGCTCC TCCCCAGATT 
GAAGATGAGG AACCTGCATT TCCACACACT GACCTGGCAA AGTTAGATGA TATGATCAAC 
AGGCCTCGCT GGGTCGTTCC AGTTTTGCCG AAAGGGGAAT TAGAAGTGCT TTTAGAAGCT 
GCTATTGATC TTAGTAAGAA AGGCCTTGAT GTCAAAAGTG AAGCATGTCA GAGATTTTTC 
CGAGATGGGC TAACAATCTC ATTCACAAAA ATCCTTACAG ATGAAGCAGT GAGTGGCTGG 
AAGTTTGAAA TTCATAGATG TATTATTAAC AATACTCATC GCCTGGTGGA GCTGTGTGTG 
GCTAAGTTAG CCCAAGACTG GTTTCCACTT CTAGAACTTC TTGCCATGGC CTTAAATCCT 
CATTGCAAAT TCCATATCTA CAATGGTACA CGTCCCTGCG AGTCAGTGTC CTCAAGTGTT 
CAGTTGCCTG AAGATGAACT CTTTGCTCGT TCTCCAGACC CTCGATCACC AAAAGGTTGG 
CTAGTGGATC TCCTCAACAA ATTTGGCACT TTAAATGGAT TCCAGATACT GCATGATCGT 
TTTATTAATG GATCAGCATT AAATGTTCAG ATAATTGCAG CCCTTATTAA ACCATTTGGA 
CAGTGCTATG AGTTTCTTAC TCTTCACACG GTGAAAAAAT ACTTTCTTCC AATAATAGAA 
ATGGTTCCAC AGTTTTTAGA AAACTTAACT GATGAAGAGC TGAAGAAAGA AGCAAAGAAT 
GAAGCCAAAA ATGATGCTCT CTCAATGATT ATTAAATCAT TGAAGAGTTT AGCTTCACGA 
GTTCCTGGAC AGGAAGAAAC TGTAAAGAAC TTAGAAATAT TTAGGTTAAA AATGATACTT 
AGATTATTGC AAATTTCTTC CTTCAATGGA AAGATGAATG CACTGAATGA GGTTAATAAG 
GTGATTTCCA GTGTGTCATA CTATACCCAT CGGCATGGCA GTTCTGAGGA AGAAGAGTGG 
CTCACAGCAG AGAGGATGGC TGAATGGATA CAGCAGAACA ATATTTTATC AATAGTTTTA 
CGAGATAGTC TTCATCAACC ACAGTATGTA GAGAAACTAG AAAAGATTCT TCGCTTTGTC 
ATAAAAGAAA AAGCTCTGAC CTTGCAAGAT CTTGATAATA TCTGGGCAGC ACAGGCAGGG 
AAACATGAAG CCATCGTAAA GAATGTCCAT GATCTACTGG CCAAATTGGC ATGGGATTTT 
TCTCCTGAAC AACTTGATCA TCTTTTTGAT TGCTTTAAGG CCAGTTGGAC AAATGCAAGT 
AAAAAGCAAC GTGAAAAGCT CCTTGAGCTG ATTCGTCGTC TTGCAGAAGA TGATAAAGAT 
GGTGTGATGG CACATAAAGT GTTGAACCTT CTGTGGAATC TAGCTCACAG TGATGATGTG 
CCTGTGGATA TCATGGACTT GGCTCTCAGT GCTCACATCA AAATACTAGA TTATAGTTGC 
TCCCAGGACC GGGACACACA AAAGATCCAG TGGATAGATC GCTTCATAGA AGAACTTCGC 
ACAAATGACA AGTGGGTCAT TCCTGCACTG AAACAAATTA GAGAAATTTG CAGTTTGTTT 
GGTGAAGCAC CTCAAAATTT GAGTCAAACT CAGAGAAGTC CCCATGTATT TTATCGCCAT 
GATTTAATCA ATCAACTTCA GCACAATCAT GCCCTAGTTA CTTTGGTAGC AGAAAACCTT 
GCAACTTACA TGGAAAGCAT GAGACTGTAT GGCAGAGACA ATGAAGACTA TGACCCACAA 
ACTGTGAGAG TGGGAAGTAG ATATAGTCAT GTTCAAGAAG TCCAAGAACG TCTTAACTTT 
CTTAGATTTT TATTGAAGGA TGGCCAGCTA TGGCTGTGTG CTCCTCAGGC AAAACAAATA 
TGGAAGTGCT TAGCAGAGAA TGCGGTTTAT CTTTGTGACC GTGAAGCCTG CTTTAAGTGG 
TATTCCAAAT TAATGGGAGA TGAACCAGAC TTAGATCCTG ATATCAATAA GGACTTCTTT 
GAAAGTAATG TGCTTCAGCT TGATCCTTCA CTATTAACTG AAAATGGAAT GAAATGTTTT 
GAGAGATTCT TCAAAGCTGT GAATTGTCGA GAAGGAAAAC TAGTAGCAAA AAGAAGAGCC 
TATATGATGG ATGATTTGGA ATTGATAGGA TTAGACTATC TTTGGAGGGT TGTAATTCAG 
AGTAATGATG ATATTGCCAG CCGAGCTATT GATCTCCTCA AAGAGATATA CACAAACCTT 
GGTCCAAGAC TGCAGGTCAA TCAGGTGGTG ATCCATGAAG ACTTCATTCA GTCTTGCTTT 
GATCGTTTGA AAGCCTCTTA TGACACGTTG TGTGTTTTGG ATGGTGACAA AGACAGTATT 
AATTGTGCAA GACAGGAAGC TGTTCGAATG GTCCGAGTAT TAACTGTTTT AAGAGAATAT 
ATAAATGAAT GTGACAGTGA TTATCATGAA GAAAGAACAA TTTTGCCTAT GTCAAGAGCT 
TTCCGTGGTA AACACCTCTC TTTTATAGTT CGGTTTCCAA ACCAGGGCAG ACAAGTAGAT 
GACTTGGAAG TTTGGTCTCA TACAAATGAT ACCATTGGTT CAGTTCGACG ATGTATACTA 
AATCGCATTA AAGCCAATGT AGCTCATACA AAAATTGAAC TCTTTGTGGG TGGTGAGCTT 
ATTGATCCTG GAGATGACAG AAAGTTGATT GGACAATTAA ACCTAAAAGA TAAATCACTA 
ATTACAGCCA AACTTACACA AATCAGCTCC AATATGCCGT CAAGTCCTGA TAGCTCCTCT 
GATTCCTCAA CTGGATCTCC TGGAAACCAT GGTAATCATT ACAGTGATGG TCCCAACCCA 
GAGGTAGAAA GCTGTTTGCC TGGAGTGATA ATGTCACTTC ATCCCAGATA CATCTCTTTC 
CTTTGGCAAG TTGCAGACTT AGGGAGCAGC CTAAATATGC CCCCTCTTAG AGATGGAGCA 
AGAGTTCTTA TGAAACTTAT GCCGCCAGAT AGTACAACAA TAGAAAAATT AAGAGCTATT 
TGTTTAGATC ATGCCAAACT TGGAGAAAGC AGCCTTAGTC CATCTCTTGA CTCACTTTTC 
TTTGGTCCTT CAGCCTCACA AGTGCTATAC CTAACAGAGG TAGTCTATGC CCTGTTAATG 
CCTGCTGGTG CACCCCTGGC TGATGACTCC TCTGATTTTC AGTTTCACTT CTTGAAAAGT 
GGTGGTTTGC CCCTTGTTCT GAGTATGCTA ACCAGAAATA ACTTCCTACC AAATGCTGAT 
ATGGAAACTC GAAGGGGTGC CTACCTCAAT GCTCTTAAAA TAGCCAAACT GTTACTAACT 
GCCATTGGCT ATGGCCATGT TCGGGCTGTG GCAGAAGCAT GTCAGCCAGG TGTAGAAGGC 
GTGAATCCTA TGACATCGGT CAACCAAGTA ACTCATGATC AAGCAGTGGT GCTACAAAGT 
GCCCTTCAGA GCATTCCTAA CCCATCATCT GAATGCATGC TTAGAAACGT GTCTGTTCGT 
CTTGCTCAGC AGATTTCTGA TGAGGCTTCA AGATATATGC CTGATATTTG TGTAATTAGA 
GCTATACAAA AGATTATTTG GACATCAGGA TGTGGGGGAT TACAACTGGT ATTCAGCCCA 
AATGAGGAAG TCACAAAAAT TTATGAGAAG ACCAATGCAG GCAATGAGCC AGACCTTGAA 
GATGAACAGG TTTGCTGTGA GGCATTGGAA GTGATGACAC TGTGTTTCGC CCTGATTCCA 
ACAGCCTTAG ATGCTCTAAG TAAAGAAAAG GCTTGGCAGA CATTTATTAT TGACTTACTG 
TTACACTGTC ACAGCAAAAC TGTCCGTCAA GTAGCACAGG AACAATTCTT CTTAATGTGC 
ACCAGATGTT GCATGGGACA CAGGCCTCTA CTTTTCTTCA TTACTCTACT CTTTACTGTT 
TTGGGGAGCA CAGCCAGAGA GAGGGCTAAA CATTCAGGCG ACTACTTTAC TCTTTTAAGG 
CATCTTCTTA ATTACGCTTA CAACAGTAAC ATTAATGTAC CCAATGCTGA AGTTCTTCTA 
AATAATGAAA TTGATTGGCT TAAAAGAATT AGGGATGATG TTAAAAGAAC AGGTGAAACA 
GGCGTTGAAG AGACAATCTT AGAAGGACAC CTTGGGGTTA CAAAGGAGCT ACTGGCTTTT 
CAGACCCCTG AGAAAAAGTT TCATATTGGT TGTGAAAAGG GGGGTGCTAA TCTCATTAAA 
GAATTAATCG ACGATTTCAT CTTTCCTGCA TCCAATGTTT ACCTCCAGTA TATGAGAAAT 
GGAGAACTCC CTGCTGAGCA GGCCATTCCT GTCTGTGGTT CACCAGCCAC AATTAATGCT 
GGTTTTGAGT TACTTGTAGC ATTAGCTGTT GGCTGTGTAA GGAACCTCAA ACAGATAGTA 
GATTCTTTGA CTGAAATGTA TTACATTGGT ACAGCAATAA CTACTTGTGA GGCACTTACT 
GAGTGGGAAT ACCTGCCACC TGTTGGACCC CGTCCACCAA AAGGATTTGT GGGGCTGAAA 
AATGCTGGTG CTACTTGTTA CATGAATTCT GTGATTCAGC AACTCTATAT GATTCCCTCT 
ATCAGGAACG GTATTCTTGC AATTGAAGGC ACAGGTAGTG ATGTAGATGA TGATATGTCT 
GGGGATGAGA AGCAGGACAA TGAGAGCAAT GTTGATCCCA GGGATGATGT GTTTGGATAT 
CCTCAACAAT TTGAAGACAA ACCACCACTG AGTAAAACAG AAGATAGAAA AGAGTACAAT 
ATTGGTGTTC TAAGACACCT TCAAGTTATC TTTGGCCATT TAGCTGCTTC TCGACTACAG 
TACTATGTGC CCAGAGGATT TTGGAAACAG TTCAGGCTTT GGGGTGAGCC TGTTAATCTT 
CGTGAACAAC ATGATGCTTT AGAGTTCTTT AATTCATTGG TGGATAGTTT AGATGAAGCT 
TTAAAAGCCT TAGGGCATCC AGCTATGCTA AGTAAAGTCT TGGGAGGTTC CTTCGCTGAT 
CAGAAAATTT GTCAAGGCTG CCCACATAGG TATGAATGTG AAGAATCTTT TACGACTCTG 
AATGTAGACA TTAGAAACCA CCAAAATCTT CTTGATTCTT TGGAACAGTA TGTCAAAGGA 
GATTTACTAG AAGGGGCAAA TGCTTATCAT TGTGAAAAAT GCAATAAAAA GGTTGATACT 
GTAAAGCGCT TGCTAATTAA AAAGTTGCCT CCTGTTCTTG CTATCCAACT AAAACGATTC 
GATTATGACT GGGAAAGAGA ATGTGCAATC AAGTTCAATG ATTACTTTGA GTTTCCTCGA 
GAGTTAGACA TGGAGCCGTA CACAGTTGCA GGTGTTGCTA AACTAGAGGG AGATAATGTC 
AACCCAGAAA GTCAGCTGAT ACAACAGAAT GAGCAGTCTG AAAGCGAAAA AGCCGGAAGC 
ACAAAATACA GACTTGTGGG TGTGCTTGTA CACAGTGGTC AAGCAAGTGG GGGACATTAC 
TATTCTTACA TCATTCAGAG GAATGGAGGA GATGGTGAAA AAAATCGTTG GTATAAATTT 
GATGATGGAG ATGTAACAGA GTGTAAAATG GATGATGATG AAGAAATGAA AAACCAGTGT 
TTTGGTGGCG AGTACATGGG AGAAGTGTTT GATCACATGA TGAAGCGCAT GTCGTACAGG 
CGGCAGAAAA GGTGGTGGAA TGCATATATA CTTTTTTATG AAAGAATGGA TACGATAGAT 
CATGATGATG AGGTGATAAG ATACATATCA GAGATTGCTA TCACCACAAG GCCCCATCAG 
ATTGTTATGC CATCGGCCAT TGAAAGAAGT GTGCGGAAGC AGAATGTCCA ATTCATGCAT 
AACCGAATGC AGTACAGTTT GGAATATTTT CAGTTTATGA AAAAGCTACT TACATGTAAT 
GGTGTTTATT TAAACCCTCC ACCAGGGCAA GATCACCTGT CTCCTGAAGC AGAAGAAATC 
ACTATGATTA GTATTCAGCT TGCTGCTAGG TTCCTTTTTA CTACAGGATT TCACACAAAG 
AAAATAGTCC GAGGATCCGC CAGTGATTGG TATGATGCAT TGTGTATTCT CCTCCGTCAC 
AGCAAGAATG TGCGGTTTTG GTTTGCTCAC AATGTGCTTT TTAATGTTTC AAATCGATTC 
TCTGAGTACC TTTTGGAATG CCCTAGTGCA GAAGTGAGAG GTGCATTTGC TAAACTTATA 
GTTTTCATTG CACACTTTTC CTTACAAGAT GGGCCTTGTC CTTCACCTTT TGCATCTCCT 
GGACCTTCTA GTCAGGCTTA TGATAACTTA AGTTTGAGTG ACCACCTACT AAGAGCAGTA 
CTAAATCTCT TGAGGAGGGA GGTTTCAGAG CATGGACGTC ATTTACAGCA GTATTTCAAC 
TTGTTTGTAA TGTATGCCAA TTTAGGAGTG GCAGAGAAAA CACAGCTGCT CAAACTGAGT 
GTACCTGCTA CCTTTATGCT TGTGTCCTTA GATGAAGGTC CTGGTCCTCC AATTAAATAT 
CAGTATGCTG AATTAGGCAA ATTATACTCC GTAGTGTCAC AGTTAATCCG CTGTTGCAAT 
GTCTCTTCAA GAATGCAGTC TTCAATCAAT GGTAATCCTT CTCTTCCAAA TCCTTTTGGT 
GATCCTAATT TATCACAACC TATAATGCCA ATTCAACAAA ATGTGGTAGA CATTTTATTT 
GTGAGAACAA GTTATGTGAA GAAAATTATT GAAGACTGCA GTAACTCTGA TGAGACCGTC 
AAATTGCTTC GCTTTTGCTG CTGGGAGAAT CCTCAGTTCT CATCTACTGT CCTGAGTGAA 
CTTCTTTGGC AGGTTGCGTA TTCCTATACT TACGAACTCC GGCCCTATTT GGATCTGCTT 
TTACAAATCT TACTGATTGA AGACTCCTGG CAGACTCACA GAATTCATAA TGCCCTTAAA 
GGAATTCCAG ATGACCGAGA TGGGCTGTTT GACACAATTC AGCGTTCTAA GAATCACTAT 
CAAAAAAGAG CATACCAATG TATAAAATGT ATGGTAGCCC TCTTTAGCAG TTGTCCTGTG 
GCTTACCAAA TCCTGCAGGG CAATGGAGAT CTTAAAAGGA AATGGACCTG GGCAGTAGAG 
TGGCTTGGAG ATGAACTCGA AAGAAGACCA TACACTGGCA ATCCTCAGTA CACTTACAAC 
AACTGGTCTC CTCCAGTGCA AAGCAATGAA ACATCAAATG GCTACTTCTT GGAGAGATCA 
CATAGCGCTA GGATGACACT TGCAAAAGCT TGTGAACTCT GTCCTGAGGA GGAACCAGAT 
GACCAAGATG CCCCAGATGA GCATGAATCA CCTCCACCTG AAGATGCCCC ATTATACCCC 
CATTCCCCTG GATCTCAATA TCAGCAGCCC TATACAGGCC CAGCCGCACA TCACATGAAC 
AACCCTCAGA GAACTGGCCA ACGAGCACAA GAAAATTATG AAGGCAGTGA AGAAGTGTCC 
CCACCTCAGA CCAAGGATCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001129365.1
CDS	500..8143
Translation	MTATTRGSPVGGNDNQGQAPDGQSQPPLQQNQTSSPDSSNENSPATPPDEQGQGDAPPQIEDEEPAFPHTDLAKLDDMINRPRWVVPVLPKGELEVLLEAAIDLSKKGLDVKSEACQRFFRDGLTISFTKILTDEAVSGWKFEIHRCIINNTHRLVELCVAKLAQDWFPLLELLAMALNPHCKFHIYNGTRPCESVSSSVQLPEDELFARSPDPRSPKGWLVDLLNKFGTLNGFQILHDRFINGSALNVQIIAALIKPFGQCYEFLTLHTVKKYFLPIIEMVPQFLENLTDEELKKEAKNEAKNDALSMIIKSLKSLASRVPGQEETVKNLEIFRLKMILRLLQISSFNGKMNALNEVNKVISSVSYYTHRHGSSEEEEWLTAERMAEWIQQNNILSIVLRDSLHQPQYVEKLEKILRFVIKEKALTLQDLDNIWAAQAGKHEAIVKNVHDLLAKLAWDFSPEQLDHLFDCFKASWTNASKKQREKLLELIRRLAEDDKDGVMAHKVLNLLWNLAHSDDVPVDIMDLALSAHIKILDYSCSQDRDTQKIQWIDRFIEELRTNDKWVIPALKQIREICSLFGEAPQNLSQTQRSPHVFYRHDLINQLQHNHALVTLVAENLATYMESMRLYGRDNEDYDPQTVRVGSRYSHVQEVQERLNFLRFLLKDGQLWLCAPQAKQIWKCLAENAVYLCDREACFKWYSKLMGDEPDLDPDINKDFFESNVLQLDPSLLTENGMKCFERFFKAVNCREGKLVAKRRAYMMDDLELIGLDYLWRVVIQSNDDIASRAIDLLKEIYTNLGPRLQVNQVVIHEDFIQSCFDRLKASYDTLCVLDGDKDSINCARQEAVRMVRVLTVLREYINECDSDYHEERTILPMSRAFRGKHLSFIVRFPNQGRQVDDLEVWSHTNDTIGSVRRCILNRIKANVAHTKIELFVGGELIDPGDDRKLIGQLNLKDKSLITAKLTQISSNMPSSPDSSSDSSTGSPGNHGNHYSDGPNPEVESCLPGVIMSLHPRYISFLWQVADLGSSLNMPPLRDGARVLMKLMPPDSTTIEKLRAICLDHAKLGESSLSPSLDSLFFGPSASQVLYLTEVVYALLMPAGAPLADDSSDFQFHFLKSGGLPLVLSMLTRNNFLPNADMETRRGAYLNALKIAKLLLTAIGYGHVRAVAEACQPGVEGVNPMTSVNQVTHDQAVVLQSALQSIPNPSSECMLRNVSVRLAQQISDEASRYMPDICVIRAIQKIIWTSGCGGLQLVFSPNEEVTKIYEKTNAGNEPDLEDEQVCCEALEVMTLCFALIPTALDALSKEKAWQTFIIDLLLHCHSKTVRQVAQEQFFLMCTRCCMGHRPLLFFITLLFTVLGSTARERAKHSGDYFTLLRHLLNYAYNSNINVPNAEVLLNNEIDWLKRIRDDVKRTGETGVEETILEGHLGVTKELLAFQTPEKKFHIGCEKGGANLIKELIDDFIFPASNVYLQYMRNGELPAEQAIPVCGSPATINAGFELLVALAVGCVRNLKQIVDSLTEMYYIGTAITTCEALTEWEYLPPVGPRPPKGFVGLKNAGATCYMNSVIQQLYMIPSIRNGILAIEGTGSDVDDDMSGDEKQDNESNVDPRDDVFGYPQQFEDKPPLSKTEDRKEYNIGVLRHLQVIFGHLAASRLQYYVPRGFWKQFRLWGEPVNLREQHDALEFFNSLVDSLDEALKALGHPAMLSKVLGGSFADQKICQGCPHRYECEESFTTLNVDIRNHQNLLDSLEQYVKGDLLEGANAYHCEKCNKKVDTVKRLLIKKLPPVLAIQLKRFDYDWERECAIKFNDYFEFPRELDMEPYTVAGVAKLEGDNVNPESQLIQQNEQSESEKAGSTKYRLVGVLVHSGQASGGHYYSYIIQRNGGDGEKNRWYKFDDGDVTECKMDDDEEMKNQCFGGEYMGEVFDHMMKRMSYRRQKRWWNAYILFYERMDTIDHDDEVIRYISEIAITTRPHQIVMPSAIERSVRKQNVQFMHNRMQYSLEYFQFMKKLLTCNGVYLNPPPGQDHLSPEAEEITMISIQLAARFLFTTGFHTKKIVRGSASDWYDALCILLRHSKNVRFWFAHNVLFNVSNRFSEYLLECPSAEVRGAFAKLIVFIAHFSLQDGPCPSPFASPGPSSQAYDNLSLSDHLLRAVLNLLRREVSEHGRHLQQYFNLFVMYANLGVAEKTQLLKLSVPATFMLVSLDEGPGPPIKYQYAELGKLYSVVSQLIRCCNVSSRMQSSINGNPSLPNPFGDPNLSQPIMPIQQNVVDILFVRTSYVKKIIEDCSNSDETVKLLRFCCWENPQFSSTVLSELLWQVAYSYTYELRPYLDLLLQILLIEDSWQTHRIHNALKGIPDDRDGLFDTIQRSKNHYQKRAYQCIKCMVALFSSCPVAYQILQGNGDLKRKWTWAVEWLGDELERRPYTGNPQYTYNNWSPPVQSNETSNGYFLERSHSARMTLAKACELCPEEEPDDQDAPDEHESPPPEDAPLYPHSPGSQYQQPYTGPAAHHMNNPQRTGQRAQENYEGSEEVSPPQTKDQ

Target ORF information:

RefSeq Version	NM_001135893.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ubiquitin specific peptidase 9, X-linked (Usp9x), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001135893.1

ORF Insert Sequence:

ATGACAGCCA CGACTCGTGG CTCTCCAGTT GGAGGGAATG ACAACCAGGG CCAAGCTCCT 
GATGGACAGT CTCAGCCCCC CCTCCAACAG AATCAGACTT CATCGCCTGA TTCATCCAAT 
GAAAATTCCC CTGCAACTCC TCCTGATGAG CAAGGCCAAG GTGATGCTCC TCCCCAGATT 
GAAGATGAGG AACCTGCATT TCCACACACT GACCTGGCAA AGTTAGATGA TATGATCAAC 
AGGCCTCGCT GGGTCGTTCC AGTTTTGCCG AAAGGGGAAT TAGAAGTGCT TTTAGAAGCT 
GCTATTGATC TTAGTAAGAA AGGCCTTGAT GTCAAAAGTG AAGCATGTCA GAGATTTTTC 
CGAGATGGGC TAACAATCTC ATTCACAAAA ATCCTTACAG ATGAAGCAGT GAGTGGCTGG 
AAGTTTGAAA TTCATAGATG TATTATTAAC AATACTCATC GCCTGGTGGA GCTGTGTGTG 
GCTAAGTTAG CCCAAGACTG GTTTCCACTT CTAGAACTTC TTGCCATGGC CTTAAATCCT 
CATTGCAAAT TCCATATCTA CAATGGTACA CGTCCCTGCG AGTCAGTGTC CTCAAGTGTT 
CAGTTGCCTG AAGATGAACT CTTTGCTCGT TCTCCAGACC CTCGATCACC AAAAGGTTGG 
CTAGTGGATC TCCTCAACAA ATTTGGCACT TTAAATGGAT TCCAGATACT GCATGATCGT 
TTTATTAATG GATCAGCATT AAATGTTCAG ATAATTGCAG CCCTTATTAA ACCATTTGGA 
CAGTGCTATG AGTTTCTTAC TCTTCACACG GTGAAAAAAT ACTTTCTTCC AATAATAGAA 
ATGGTTCCAC AGTTTTTAGA AAACTTAACT GATGAAGAGC TGAAGAAAGA AGCAAAGAAT 
GAAGCCAAAA ATGATGCTCT CTCAATGATT ATTAAATCAT TGAAGAGTTT AGCTTCACGA 
GTTCCTGGAC AGGAAGAAAC TGTAAAGAAC TTAGAAATAT TTAGGTTAAA AATGATACTT 
AGATTATTGC AAATTTCTTC CTTCAATGGA AAGATGAATG CACTGAATGA GGTTAATAAG 
GTGATTTCCA GTGTGTCATA CTATACCCAT CGGCATGGCA GTTCTGAGGA AGAAGAGTGG 
CTCACAGCAG AGAGGATGGC TGAATGGATA CAGCAGAACA ATATTTTATC AATAGTTTTA 
CGAGATAGTC TTCATCAACC ACAGTATGTA GAGAAACTAG AAAAGATTCT TCGCTTTGTC 
ATAAAAGAAA AAGCTCTGAC CTTGCAAGAT CTTGATAATA TCTGGGCAGC ACAGGCAGGG 
AAACATGAAG CCATCGTAAA GAATGTCCAT GATCTACTGG CCAAATTGGC ATGGGATTTT 
TCTCCTGAAC AACTTGATCA TCTTTTTGAT TGCTTTAAGG CCAGTTGGAC AAATGCAAGT 
AAAAAGCAAC GTGAAAAGCT CCTTGAGCTG ATTCGTCGTC TTGCAGAAGA TGATAAAGAT 
GGTGTGATGG CACATAAAGT GTTGAACCTT CTGTGGAATC TAGCTCACAG TGATGATGTG 
CCTGTGGATA TCATGGACTT GGCTCTCAGT GCTCACATCA AAATACTAGA TTATAGTTGC 
TCCCAGGACC GGGACACACA AAAGATCCAG TGGATAGATC GCTTCATAGA AGAACTTCGC 
ACAAATGACA AGTGGGTCAT TCCTGCACTG AAACAAATTA GAGAAATTTG CAGTTTGTTT 
GGTGAAGCAC CTCAAAATTT GAGTCAAACT CAGAGAAGTC CCCATGTATT TTATCGCCAT 
GATTTAATCA ATCAACTTCA GCACAATCAT GCCCTAGTTA CTTTGGTAGC AGAAAACCTT 
GCAACTTACA TGGAAAGCAT GAGACTGTAT GGCAGAGACA ATGAAGACTA TGACCCACAA 
ACTGTGAGAG TGGGAAGTAG ATATAGTCAT GTTCAAGAAG TCCAAGAACG TCTTAACTTT 
CTTAGATTTT TATTGAAGGA TGGCCAGCTA TGGCTGTGTG CTCCTCAGGC AAAACAAATA 
TGGAAGTGCT TAGCAGAGAA TGCGGTTTAT CTTTGTGACC GTGAAGCCTG CTTTAAGTGG 
TATTCCAAAT TAATGGGAGA TGAACCAGAC TTAGATCCTG ATATCAATAA GGACTTCTTT 
GAAAGTAATG TGCTTCAGCT TGATCCTTCA CTATTAACTG AAAATGGAAT GAAATGTTTT 
GAGAGATTCT TCAAAGCTGT GAATTGTCGA GAAGGAAAAC TAGTAGCAAA AAGAAGAGCC 
TATATGATGG ATGATTTGGA ATTGATAGGA TTAGACTATC TTTGGAGGGT TGTAATTCAG 
AGTAATGATG ATATTGCCAG CCGAGCTATT GATCTCCTCA AAGAGATATA CACAAACCTT 
GGTCCAAGAC TGCAGGTCAA TCAGGTGGTG ATCCATGAAG ACTTCATTCA GTCTTGCTTT 
GATCGTTTGA AAGCCTCTTA TGACACGTTG TGTGTTTTGG ATGGTGACAA AGACAGTATT 
AATTGTGCAA GACAGGAAGC TGTTCGAATG GTCCGAGTAT TAACTGTTTT AAGAGAATAT 
ATAAATGAAT GTGACAGTGA TTATCATGAA GAAAGAACAA TTTTGCCTAT GTCAAGAGCT 
TTCCGTGGTA AACACCTCTC TTTTATAGTT CGGTTTCCAA ACCAGGGCAG ACAAGTAGAT 
GACTTGGAAG TTTGGTCTCA TACAAATGAT ACCATTGGTT CAGTTCGACG ATGTATACTA 
AATCGCATTA AAGCCAATGT AGCTCATACA AAAATTGAAC TCTTTGTGGG TGGTGAGCTT 
ATTGATCCTG GAGATGACAG AAAGTTGATT GGACAATTAA ACCTAAAAGA TAAATCACTA 
ATTACAGCCA AACTTACACA AATCAGCTCC AATATGCCGT CAAGTCCTGA TAGCTCCTCT 
GATTCCTCAA CTGGATCTCC TGGAAACCAT GGTAATCATT ACAGTGATGG TCCCAACCCA 
GAGGTAGAAA GCTGTTTGCC TGGAGTGATA ATGTCACTTC ATCCCAGATA CATCTCTTTC 
CTTTGGCAAG TTGCAGACTT AGGGAGCAGC CTAAATATGC CCCCTCTTAG AGATGGAGCA 
AGAGTTCTTA TGAAACTTAT GCCGCCAGAT AGTACAACAA TAGAAAAATT AAGAGCTATT 
TGTTTAGATC ATGCCAAACT TGGAGAAAGC AGCCTTAGTC CATCTCTTGA CTCACTTTTC 
TTTGGTCCTT CAGCCTCACA AGTGCTATAC CTAACAGAGG TAGTCTATGC CCTGTTAATG 
CCTGCTGGTG CACCCCTGGC TGATGACTCC TCTGATTTTC AGTTTCACTT CTTGAAAAGT 
GGTGGTTTGC CCCTTGTTCT GAGTATGCTA ACCAGAAATA ACTTCCTACC AAATGCTGAT 
ATGGAAACTC GAAGGGGTGC CTACCTCAAT GCTCTTAAAA TAGCCAAACT GTTACTAACT 
GCCATTGGCT ATGGCCATGT TCGGGCTGTG GCAGAAGCAT GTCAGCCAGG TGTAGAAGGC 
GTGAATCCTA TGACATCGGT CAACCAAGTA ACTCATGATC AAGCAGTGGT GCTACAAAGT 
GCCCTTCAGA GCATTCCTAA CCCATCATCT GAATGCATGC TTAGAAACGT GTCTGTTCGT 
CTTGCTCAGC AGATTTCTGA TGAGGCTTCA AGATATATGC CTGATATTTG TGTAATTAGA 
GCTATACAAA AGATTATTTG GACATCAGGA TGTGGGGGAT TACAACTGGT ATTCAGCCCA 
AATGAGGAAG TCACAAAAAT TTATGAGAAG ACCAATGCAG GCAATGAGCC AGACCTTGAA 
GATGAACAGG TTTGCTGTGA GGCATTGGAA GTGATGACAC TGTGTTTCGC CCTGATTCCA 
ACAGCCTTAG ATGCTCTAAG TAAAGAAAAG GCTTGGCAGA CATTTATTAT TGACTTACTG 
TTACACTGTC ACAGCAAAAC TGTCCGTCAA GTAGCACAGG AACAATTCTT CTTAATGTGC 
ACCAGATGTT GCATGGGACA CAGGCCTCTA CTTTTCTTCA TTACTCTACT CTTTACTGTT 
TTGGGGAGCA CAGCCAGAGA GAGGGCTAAA CATTCAGGCG ACTACTTTAC TCTTTTAAGG 
CATCTTCTTA ATTACGCTTA CAACAGTAAC ATTAATGTAC CCAATGCTGA AGTTCTTCTA 
AATAATGAAA TTGATTGGCT TAAAAGAATT AGGGATGATG TTAAAAGAAC AGGTGAAACA 
GGCGTTGAAG AGACAATCTT AGAAGGACAC CTTGGGGTTA CAAAGGAGCT ACTGGCTTTT 
CAGACCCCTG AGAAAAAGTT TCATATTGGT TGTGAAAAGG GGGGTGCTAA TCTCATTAAA 
GAATTAATCG ACGATTTCAT CTTTCCTGCA TCCAATGTTT ACCTCCAGTA TATGAGAAAT 
GGAGAACTCC CTGCTGAGCA GGCCATTCCT GTCTGTGGTT CACCAGCCAC AATTAATGCT 
GGTTTTGAGT TACTTGTAGC ATTAGCTGTT GGCTGTGTAA GGAACCTCAA ACAGATAGTA 
GATTCTTTGA CTGAAATGTA TTACATTGGT ACAGCAATAA CTACTTGTGA GGCACTTACT 
GAGTGGGAAT ACCTGCCACC TGTTGGACCC CGTCCACCAA AAGGATTTGT GGGGCTGAAA 
AATGCTGGTG CTACTTGTTA CATGAATTCT GTGATTCAGC AACTCTATAT GATTCCCTCT 
ATCAGGAACG GTATTCTTGC AATTGAAGGC ACAGGTAGTG ATGTAGATGA TGATATGTCT 
GGGGATGAGA AGCAGGACAA TGAGAGCAAT GTTGATCCCA GGGATGATGT GTTTGGATAT 
CCTCAACAAT TTGAAGACAA ACCACCACTG AGTAAAACAG AAGATAGAAA AGAGTACAAT 
ATTGGTGTTC TAAGACACCT TCAAGTTATC TTTGGCCATT TAGCTGCTTC TCGACTACAG 
TACTATGTGC CCAGAGGATT TTGGAAACAG TTCAGGCTTT GGGGTGAGCC TGTTAATCTT 
CGTGAACAAC ATGATGCTTT AGAGTTCTTT AATTCATTGG TGGATAGTTT AGATGAAGCT 
TTAAAAGCCT TAGGGCATCC AGCTATGCTA AGTAAAGTCT TGGGAGGTTC CTTCGCTGAT 
CAGAAAATTT GTCAAGGCTG CCCACATAGG TATGAATGTG AAGAATCTTT TACGACTCTG 
AATGTAGACA TTAGAAACCA CCAAAATCTT CTTGATTCTT TGGAACAGTA TGTCAAAGGA 
GATTTACTAG AAGGGGCAAA TGCTTATCAT TGTGAAAAAT GCAATAAAAA GGTTGATACT 
GTAAAGCGCT TGCTAATTAA AAAGTTGCCT CCTGTTCTTG CTATCCAACT AAAACGATTC 
GATTATGACT GGGAAAGAGA ATGTGCAATC AAGTTCAATG ATTACTTTGA GTTTCCTCGA 
GAGTTAGACA TGGAGCCGTA CACAGTTGCA GGTGTTGCTA AACTAGAGGG AGATAATGTC 
AACCCAGAAA GTCAGCTGAT ACAACAGAAT GAGCAGTCTG AAAGCGAAAA AGCCGGAAGC 
ACAAAATACA GACTTGTGGG TGTGCTTGTA CACAGTGGTC AAGCAAGTGG GGGACATTAC 
TATTCTTACA TCATTCAGAG GAATGGAGGA GATGGTGAAA AAAATCGTTG GTATAAATTT 
GATGATGGAG ATGTAACAGA GTGTAAAATG GATGATGATG AAGAAATGAA AAACCAGTGT 
TTTGGTGGCG AGTACATGGG AGAAGTGTTT GATCACATGA TGAAGCGCAT GTCGTACAGG 
CGGCAGAAAA GGTGGTGGAA TGCATATATA CTTTTTTATG AAAGAATGGA TACGATAGAT 
CATGATGATG AGGTGATAAG ATACATATCA GAGATTGCTA TCACCACAAG GCCCCATCAG 
ATTGTTATGC CATCGGCCAT TGAAAGAAGT GTGCGGAAGC AGAATGTCCA ATTCATGCAT 
AACCGAATGC AGTACAGTTT GGAATATTTT CAGTTTATGA AAAAGCTACT TACATGTAAT 
GGTGTTTATT TAAACCCTCC ACCAGGGCAA GATCACCTGT CTCCTGAAGC AGAAGAAATC 
ACTATGATTA GTATTCAGCT TGCTGCTAGG TTCCTTTTTA CTACAGGATT TCACACAAAG 
AAAATAGTCC GAGGATCCGC CAGTGATTGG TATGATGCAT TGTGTATTCT CCTCCGTCAC 
AGCAAGAATG TGCGGTTTTG GTTTGCTCAC AATGTGCTTT TTAATGTTTC AAATCGATTC 
TCTGAGTACC TTTTGGAATG CCCTAGTGCA GAAGTGAGAG GTGCATTTGC TAAACTTATA 
GTTTTCATTG CACACTTTTC CTTACAAGAT GGGCCTTGTC CTTCACCTTT TGCATCTCCT 
GGACCTTCTA GTCAGGCTTA TGATAACTTA AGTTTGAGTG ACCACCTACT AAGAGCAGTA 
CTAAATCTCT TGAGGAGGGA GGTTTCAGAG CATGGACGTC ATTTACAGCA GTATTTCAAC 
TTGTTTGTAA TGTATGCCAA TTTAGGAGTG GCAGAGAAAA CACAGCTGCT CAAACTGAGT 
GTACCTGCTA CCTTTATGCT TGTGTCCTTA GATGAAGGTC CTGGTCCTCC AATTAAATAT 
CAGTATGCTG AATTAGGCAA ATTATACTCC GTAGTGTCAC AGTTAATCCG CTGTTGCAAT 
GTCTCTTCAA GAATGCAGTC TTCAATCAAT GGTAATCCTT CTCTTCCAAA TCCTTTTGGT 
GATCCTAATT TATCACAACC TATAATGCCA ATTCAACAAA ATGTGGTAGA CATTTTATTT 
GTGAGAACAA GTTATGTGAA GAAAATTATT GAAGACTGCA GTAACTCTGA TGAGACCGTC 
AAATTGCTTC GCTTTTGCTG CTGGGAGAAT CCTCAGTTCT CATCTACTGT CCTGAGTGAA 
CTTCTTTGGC AGGTTGCGTA TTCCTATACT TACGAACTCC GGCCCTATTT GGATCTGCTT 
TTACAAATCT TACTGATTGA AGACTCCTGG CAGACTCACA GAATTCATAA TGCCCTTAAA 
GGAATTCCAG ATGACCGAGA TGGGCTGTTT GACACAATTC AGCGTTCTAA GAATCACTAT 
CAAAAAAGAG CATACCAATG TATAAAATGT ATGGTAGCCC TCTTTAGCAG TTGTCCTGTG 
GCTTACCAAA TCCTGCAGGG CAATGGAGAT CTTAAAAGGA AATGGACCTG GGCAGTAGAG 
TGGCTTGGAG ATGAACTCGA AAGAAGACCA TACACTGGCA ATCCTCAGTA CACTTACAAC 
AACTGGTCTC CTCCAGTGCA AAGCAATGAA ACATCAAATG GCTACTTCTT GGAGAGATCA 
CATAGCGCTA GGATGACACT TGCAAAAGCT TGTGAACTCT GTCCTGAGGA GGAACCAGAT 
GACCAAGATG CCCCAGATGA GCATGAATCA CCTCCACCTG AAGATGCCCC ATTATACCCC 
CATTCCCCTG GATCTCAATA TCAGCAGCCC TATACAGGCC CAGCCGCACA TCACATGAAC 
AACCCTCAGA GAACTGGCCA ACGAGCACAA GAAAATTATG AAGGCAGTGA AGAAGTGTCC 
CCACCTCAGA CCAAGGATCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usp9x ( XM_006256670.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006256732.1 Rattus norvegicus ubiquitin specific peptidase 9, X-linked (Usp9x), transcript variant X1, mRNA.

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CloneID	ORa30228
Clone ID Related Accession (Same CDS sequence)	NM_001135923.1 , XM_006256670.3
Accession Version	XM_006256670.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7665bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	probable ubiquitin carboxyl-terminal hydrolase FAF-X isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) and transcript sequence (NM_001135893.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006256670.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 4 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGACAGCCA CGACTCGTGG CTCTCCAGTT GGAGGGAATG ACAACCAGGG CCAAGCTCCT 
GATGGACAGT CTCAGCCCCC CCTCCAACAG AATCAGACTT CATCGCCTGA TTCATCCAAT 
GAAAATTCCC CTGCAACTCC TCCTGATGAG CAAGGCCAAG GTGATGCTCC TCCCCAGATT 
GAAGATGAGG AACCTGCATT TCCACACACT GACCTGGCAA AGTTAGATGA TATGATCAAC 
AGGCCTCGCT GGGTCGTTCC AGTTTTGCCG AAAGGGGAAT TAGAAGTGCT TTTAGAAGCT 
GCTATTGATC TTAGTAAGAA AGGCCTTGAT GTCAAAAGTG AAGCATGTCA GAGATTTTTC 
CGAGATGGGC TAACAATCTC ATTCACAAAA ATCCTTACAG ATGAAGCAGT GAGTGGCTGG 
AAGTTTGAAA TTCATAGATG TATTATTAAC AATACTCATC GCCTGGTGGA GCTGTGTGTG 
GCTAAGTTAG CCCAAGACTG GTTTCCACTT CTAGAACTTC TTGCCATGGC CTTAAATCCT 
CATTGCAAAT TCCATATCTA CAATGGTACA CGTCCCTGCG AGTCAGTGTC CTCAAGTGTT 
CAGTTGCCTG AAGATGAACT CTTTGCTCGT TCTCCAGACC CTCGATCACC AAAAGGTTGG 
CTAGTGGATC TCCTCAACAA ATTTGGCACT TTAAATGGAT TCCAGATACT GCATGATCGT 
TTTATTAATG GATCAGCATT AAATGTTCAG ATAATTGCAG CCCTTATTAA ACCATTTGGA 
CAGTGCTATG AGTTTCTTAC TCTTCACACG GTGAAAAAAT ACTTTCTTCC AATAATAGAA 
ATGGTTCCAC AGTTTTTAGA AAACTTAACT GATGAAGAGC TGAAGAAAGA AGCAAAGAAT 
GAAGCCAAAA ATGATGCTCT CTCAATGATT ATTAAATCAT TGAAGAGTTT AGCTTCACGA 
GTTCCTGGAC AGGAAGAAAC TGTAAAGAAC TTAGAAATAT TTAGGTTAAA AATGATACTT 
AGATTATTGC AAATTTCTTC CTTCAATGGA AAGATGAATG CACTGAATGA GGTTAATAAG 
GTGATTTCCA GTGTGTCATA CTATACCCAT CGGCATGGCA GTTCTGAGGA AGAAGAGTGG 
CTCACAGCAG AGAGGATGGC TGAATGGATA CAGCAGAACA ATATTTTATC AATAGTTTTA 
CGAGATAGTC TTCATCAACC ACAGTATGTA GAGAAACTAG AAAAGATTCT TCGCTTTGTC 
ATAAAAGAAA AAGCTCTGAC CTTGCAAGAT CTTGATAATA TCTGGGCAGC ACAGGCAGGG 
AAACATGAAG CCATCGTAAA GAATGTCCAT GATCTACTGG CCAAATTGGC ATGGGATTTT 
TCTCCTGAAC AACTTGATCA TCTTTTTGAT TGCTTTAAGG CCAGTTGGAC AAATGCAAGT 
AAAAAGCAAC GTGAAAAGCT CCTTGAGCTG ATTCGTCGTC TTGCAGAAGA TGATAAAGAT 
GGTGTGATGG CACATAAAGT GTTGAACCTT CTGTGGAATC TAGCTCACAG TGATGATGTG 
CCTGTGGATA TCATGGACTT GGCTCTCAGT GCTCACATCA AAATACTAGA TTATAGTTGC 
TCCCAGGACC GGGACACACA AAAGATCCAG TGGATAGATC GCTTCATAGA AGAACTTCGC 
ACAAATGACA AGTGGGTCAT TCCTGCACTG AAACAAATTA GAGAAATTTG CAGTTTGTTT 
GGTGAAGCAC CTCAAAATTT GAGTCAAACT CAGAGAAGTC CCCATGTATT TTATCGCCAT 
GATTTAATCA ATCAACTTCA GCACAATCAT GCCCTAGTTA CTTTGGTAGC AGAAAACCTT 
GCAACTTACA TGGAAAGCAT GAGACTGTAT GGCAGAGACA ATGAAGACTA TGACCCACAA 
ACTGTGAGAG TGGGAAGTAG ATATAGTCAT GTTCAAGAAG TCCAAGAACG TCTTAACTTT 
CTTAGATTTT TATTGAAGGA TGGCCAGCTA TGGCTGTGTG CTCCTCAGGC AAAACAAATA 
TGGAAGTGCT TAGCAGAGAA TGCGGTTTAT CTTTGTGACC GTGAAGCCTG CTTTAAGTGG 
TATTCCAAAT TAATGGGAGA TGAACCAGAC TTAGATCCTG ATATCAATAA GGACTTCTTT 
GAAAGTAATG TGCTTCAGCT TGATCCTTCA CTATTAACTG AAAATGGAAT GAAATGTTTT 
GAGAGATTCT TCAAAGCTGT GAATTGTCGA GAAGGAAAAC TAGTAGCAAA AAGAAGAGCC 
TATATGATGG ATGATTTGGA ATTGATAGGA TTAGACTATC TTTGGAGGGT TGTAATTCAG 
AGTAATGATG ATATTGCCAG CCGAGCTATT GATCTCCTCA AAGAGATATA CACAAACCTT 
GGTCCAAGAC TGCAGGTCAA TCAGGTGGTG ATCCATGAAG ACTTCATTCA GTCTTGCTTT 
GATCGTTTGA AAGCCTCTTA TGACACGTTG TGTGTTTTGG ATGGTGACAA AGACAGTATT 
AATTGTGCAA GACAGGAAGC TGTTCGAATG GTCCGAGTAT TAACTGTTTT AAGAGAATAT 
ATAAATGAAT GTGACAGTGA TTATCATGAA GAAAGAACAA TTTTGCCTAT GTCAAGAGCT 
TTCCGTGGTA AACACCTCTC TTTTATAGTT CGGTTTCCAA ACCAGGGCAG ACAAGTAGAT 
GACTTGGAAG TTTGGTCTCA TACAAATGAT ACCATTGGTT CAGTTCGACG ATGTATACTA 
AATCGCATTA AAGCCAATGT AGCTCATACA AAAATTGAAC TCTTTGTGGG TGGTGAGCTT 
ATTGATCCTG GAGATGACAG AAAGTTGATT GGACAATTAA ACCTAAAAGA TAAATCACTA 
ATTACAGCCA AACTTACACA AATCAGCTCC AATATGCCGT CAAGTCCTGA TAGCTCCTCT 
GATTCCTCAA CTGGATCTCC TGGAAACCAT GGTAATCATT ACAGTGATGG TCCCAACCCA 
GAGGTAGAAA GCTGTTTGCC TGGAGTGATA ATGTCACTTC ATCCCAGATA CATCTCTTTC 
CTTTGGCAAG TTGCAGACTT AGGGAGCAGC CTAAATATGC CCCCTCTTAG AGATGGAGCA 
AGAGTTCTTA TGAAACTTAT GCCGCCAGAT AGTACAACAA TAGAAAAATT AAGAGCTATT 
TGTTTAGATC ATGCCAAACT TGGAGAAAGC AGCCTTAGTC CATCTCTTGA CTCACTTTTC 
TTTGGTCCTT CAGCCTCACA AGTGCTATAC CTAACAGAGG TAGTCTATGC CCTGTTAATG 
CCTGCTGGTG CACCCCTGGC TGATGACTCC TCTGATTTTC AGTTTCACTT CTTGAAAAGT 
GGTGGTTTGC CCCTTGTTCT GAGTATGCTA ACCAGAAATA ACTTCCTACC AAATGCTGAT 
ATGGAAACTC GAAGGGGTGC CTACCTCAAT GCTCTTAAAA TAGCCAAACT GTTACTAACT 
GCCATTGGCT ATGGCCATGT TCGGGCTGTG GCAGAAGCAT GTCAGCCAGG TGTAGAAGGC 
GTGAATCCTA TGACATCGGT CAACCAAGTA ACTCATGATC AAGCAGTGGT GCTACAAAGT 
GCCCTTCAGA GCATTCCTAA CCCATCATCT GAATGCATGC TTAGAAACGT GTCTGTTCGT 
CTTGCTCAGC AGATTTCTGA TGAGGCTTCA AGATATATGC CTGATATTTG TGTAATTAGA 
GCTATACAAA AGATTATTTG GACATCAGGA TGTGGGGGAT TACAACTGGT ATTCAGCCCA 
AATGAGGAAG TCACAAAAAT TTATGAGAAG ACCAATGCAG GCAATGAGCC AGACCTTGAA 
GATGAACAGG TTTGCTGTGA GGCATTGGAA GTGATGACAC TGTGTTTCGC CCTGATTCCA 
ACAGCCTTAG ATGCTCTAAG TAAAGAAAAG GCTTGGCAGA CATTTATTAT TGACTTACTG 
TTACACTGTC ACAGCAAAAC TGTCCGTCAA GTAGCACAGG AACAATTCTT CTTAATGTGC 
ACCAGATGTT GCATGGGACA CAGGCCTCTA CTTTTCTTCA TTACTCTACT CTTTACTGTT 
TTGGGGAGCA CAGCCAGAGA GAGGGCTAAA CATTCAGGCG ACTACTTTAC TCTTTTAAGG 
CATCTTCTTA ATTACGCTTA CAACAGTAAC ATTAATGTAC CCAATGCTGA AGTTCTTCTA 
AATAATGAAA TTGATTGGCT TAAAAGAATT AGGGATGATG TTAAAAGAAC AGGTGAAACA 
GGCGTTGAAG AGACAATCTT AGAAGGACAC CTTGGGGTTA CAAAGGAGCT ACTGGCTTTT 
CAGACCCCTG AGAAAAAGTT TCATATTGGT TGTGAAAAGG GGGGTGCTAA TCTCATTAAA 
GAATTAATCG ACGATTTCAT CTTTCCTGCA TCCAATGTTT ACCTCCAGTA TATGAGAAAT 
GGAGAACTCC CTGCTGAGCA GGCCATTCCT GTCTGTGGTT CACCAGCCAC AATTAATGCT 
GGTTTTGAGT TACTTGTAGC ATTAGCTGTT GGCTGTGTAA GGAACCTCAA ACAGATAGTA 
GATTCTTTGA CTGAAATGTA TTACATTGGT ACAGCAATAA CTACTTGTGA GGCACTTACT 
GAGTGGGAAT ACCTGCCACC TGTTGGACCC CGTCCACCAA AAGGATTTGT GGGGCTGAAA 
AATGCTGGTG CTACTTGTTA CATGAATTCT GTGATTCAGC AACTCTATAT GATTCCCTCT 
ATCAGGAACG GTATTCTTGC AATTGAAGGC ACAGGTAGTG ATGTAGATGA TGATATGTCT 
GGGGATGAGA AGCAGGACAA TGAGAGCAAT GTTGATCCCA GGGATGATGT GTTTGGATAT 
CCTCAACAAT TTGAAGACAA ACCACCACTG AGTAAAACAG AAGATAGAAA AGAGTACAAT 
ATTGGTGTTC TAAGACACCT TCAAGTTATC TTTGGCCATT TAGCTGCTTC TCGACTACAG 
TACTATGTGC CCAGAGGATT TTGGAAACAG TTCAGGCTTT GGGGTGAGCC TGTTAATCTT 
CGTGAACAAC ATGATGCTTT AGAGTTCTTT AATTCATTGG TGGATAGTTT AGATGAAGCT 
TTAAAAGCCT TAGGGCATCC AGCTATGCTA AGTAAAGTCT TGGGAGGTTC CTTCGCTGAT 
CAGAAAATTT GTCAAGGCTG CCCACATAGG TATGAATGTG AAGAATCTTT TACGACTCTG 
AATGTAGACA TTAGAAACCA CCAAAATCTT CTTGATTCTT TGGAACAGTA TGTCAAAGGA 
GATTTACTAG AAGGGGCAAA TGCTTATCAT TGTGAAAAAT GCAATAAAAA GGTTGATACT 
GTAAAGCGCT TGCTAATTAA AAAGTTGCCT CCTGTTCTTG CTATCCAACT AAAACGATTC 
GATTATGACT GGGAAAGAGA ATGTGCAATC AAGTTCAATG ATTACTTTGA GTTTCCTCGA 
GAGTTAGACA TGGAGCCGTA CACAGTTGCA GGTGTTGCTA AACTAGAGGG AGATAATGTC 
AACCCAGAAA GTCAGCTGAT ACAACAGAAT GAGCAGTCTG AAAGCGAAAA AGCCGGAAGC 
ACAAAATACA GACTTGTGGG TGTGCTTGTA CACAGTGGTC AAGCAAGTGG GGGACATTAC 
TATTCTTACA TCATTCAGAG GAATGGAGGA GATGGTGAAA AAAATCGTTG GTATAAATTT 
GATGATGGAG ATGTAACAGA GTGTAAAATG GATGATGATG AAGAAATGAA AAACCAGTGT 
TTTGGTGGCG AGTACATGGG AGAAGTGTTT GATCACATGA TGAAGCGCAT GTCGTACAGG 
CGGCAGAAAA GGTGGTGGAA TGCATATATA CTTTTTTATG AAAGAATGGA TACGATAGAT 
CATGATGATG AGGTGATAAG ATACATATCA GAGATTGCTA TCACCACAAG GCCCCATCAG 
ATTGTTATGC CATCGGCCAT TGAAAGAAGT GTGCGGAAGC AGAATGTCCA ATTCATGCAT 
AACCGAATGC AGTACAGTTT GGAATATTTT CAGTTTATGA AAAAGCTACT TACATGTAAT 
GGTGTTTATT TAAACCCTCC ACCAGGGCAA GATCACCTGT CTCCTGAAGC AGAAGAAATC 
ACTATGATTA GTATTCAGCT TGCTGCTAGG TTCCTTTTTA CTACAGGATT TCACACAAAG 
AAAATAGTCC GAGGATCCGC CAGTGATTGG TATGATGCAT TGTGTATTCT CCTCCGTCAC 
AGCAAGAATG TGCGGTTTTG GTTTGCTCAC AATGTGCTTT TTAATGTTTC AAATCGATTC 
TCTGAGTACC TTTTGGAATG CCCTAGTGCA GAAGTGAGAG GTGCATTTGC TAAACTTATA 
GTTTTCATTG CACACTTTTC CTTACAAGAT GGGCCTTGTC CTTCACCTTT TGCATCTCCT 
GGACCTTCTA GTCAGGCTTA TGATAACTTA AGTTTGAGTG ACCACCTACT AAGAGCAGTA 
CTAAATCTCT TGAGGAGGGA GGTTTCAGAG CATGGACGTC ATTTACAGCA GTATTTCAAC 
TTGTTTGTAA TGTATGCCAA TTTAGGAGTG GCAGAGAAAA CACAGCTGCT CAAACTGAGT 
GTACCTGCTA CCTTTATGCT TGTGTCCTTA GATGAAGGTC CTGGTCCTCC AATTAAATAT 
CAGTATGCTG AATTAGGCAA ATTATACTCC GTAGTGTCAC AGTTAATCCG CTGTTGCAAT 
GTCTCTTCAA GAATGCAGTC TTCAATCAAT GGTAATCCTT CTCTTCCAAA TCCTTTTGGT 
GATCCTAATT TATCACAACC TATAATGCCA ATTCAACAAA ATGTGGTAGA CATTTTATTT 
GTGAGAACAA GTTATGTGAA GAAAATTATT GAAGACTGCA GTAACTCTGA TGAGACCGTC 
AAATTGCTTC GCTTTTGCTG CTGGGAGAAT CCTCAGTTCT CATCTACTGT CCTGAGTGAA 
CTTCTTTGGC AGGTTGCGTA TTCCTATACT TACGAACTCC GGCCCTATTT GGATCTGCTT 
TTACAAATCT TACTGATTGA AGACTCCTGG CAGACTCACA GAATTCATAA TGCCCTTAAA 
GGAATTCCAG ATGACCGAGA TGGGCTGTTT GACACAATTC AGCGTTCTAA GAATCACTAT 
CAAAAAAGAG CATACCAATG TATAAAATGT ATGGTAGCCC TCTTTAGCAG TTGTCCTGTG 
GCTTACCAAA TCCTGCAGGG CAATGGAGAT CTTAAAAGGA AATGGACCTG GGCAGTAGAG 
TGGCTTGGAG ATGAACTCGA AAGAAGACCA TACACTGGCA ATCCTCAGTA CACTTACAAC 
AACTGGTCTC CTCCAGTGCA AAGCAATGAA ACATCAAATG GCTACTTCTT GGAGAGATCA 
CATAGCGCTA GGATGACACT TGCAAAAGCT TGTGAACTCT GTCCTGAGGA GGAACCAGAT 
GACCAAGATG CCCCAGATGA GCATGAATCA CCTCCACCTG AAGATGCCCC ATTATACCCC 
CATTCCCCTG GATCTCAATA TCAGCAGAAT AACCATGTGC ATGGACAGCC CTATACAGGC 
CCAGCCGCAC ATCACATGAA CAACCCTCAG AGAACTGGCC AACGAGCACA AGAAAATTAT 
GAAGGCAGTG AAGAAGTGTC CCCACCTCAG ACCAAGGATC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006256732.1
CDS	1045..8709
Translation	MTATTRGSPVGGNDNQGQAPDGQSQPPLQQNQTSSPDSSNENSPATPPDEQGQGDAPPQIEDEEPAFPHTDLAKLDDMINRPRWVVPVLPKGELEVLLEAAIDLSKKGLDVKSEACQRFFRDGLTISFTKILTDEAVSGWKFEIHRCIINNTHRLVELCVAKLAQDWFPLLELLAMALNPHCKFHIYNGTRPCESVSSSVQLPEDELFARSPDPRSPKGWLVDLLNKFGTLNGFQILHDRFINGSALNVQIIAALIKPFGQCYEFLTLHTVKKYFLPIIEMVPQFLENLTDEELKKEAKNEAKNDALSMIIKSLKSLASRVPGQEETVKNLEIFRLKMILRLLQISSFNGKMNALNEVNKVISSVSYYTHRHGSSEEEEWLTAERMAEWIQQNNILSIVLRDSLHQPQYVEKLEKILRFVIKEKALTLQDLDNIWAAQAGKHEAIVKNVHDLLAKLAWDFSPEQLDHLFDCFKASWTNASKKQREKLLELIRRLAEDDKDGVMAHKVLNLLWNLAHSDDVPVDIMDLALSAHIKILDYSCSQDRDTQKIQWIDRFIEELRTNDKWVIPALKQIREICSLFGEAPQNLSQTQRSPHVFYRHDLINQLQHNHALVTLVAENLATYMESMRLYGRDNEDYDPQTVRVGSRYSHVQEVQERLNFLRFLLKDGQLWLCAPQAKQIWKCLAENAVYLCDREACFKWYSKLMGDEPDLDPDINKDFFESNVLQLDPSLLTENGMKCFERFFKAVNCREGKLVAKRRAYMMDDLELIGLDYLWRVVIQSNDDIASRAIDLLKEIYTNLGPRLQVNQVVIHEDFIQSCFDRLKASYDTLCVLDGDKDSINCARQEAVRMVRVLTVLREYINECDSDYHEERTILPMSRAFRGKHLSFIVRFPNQGRQVDDLEVWSHTNDTIGSVRRCILNRIKANVAHTKIELFVGGELIDPGDDRKLIGQLNLKDKSLITAKLTQISSNMPSSPDSSSDSSTGSPGNHGNHYSDGPNPEVESCLPGVIMSLHPRYISFLWQVADLGSSLNMPPLRDGARVLMKLMPPDSTTIEKLRAICLDHAKLGESSLSPSLDSLFFGPSASQVLYLTEVVYALLMPAGAPLADDSSDFQFHFLKSGGLPLVLSMLTRNNFLPNADMETRRGAYLNALKIAKLLLTAIGYGHVRAVAEACQPGVEGVNPMTSVNQVTHDQAVVLQSALQSIPNPSSECMLRNVSVRLAQQISDEASRYMPDICVIRAIQKIIWTSGCGGLQLVFSPNEEVTKIYEKTNAGNEPDLEDEQVCCEALEVMTLCFALIPTALDALSKEKAWQTFIIDLLLHCHSKTVRQVAQEQFFLMCTRCCMGHRPLLFFITLLFTVLGSTARERAKHSGDYFTLLRHLLNYAYNSNINVPNAEVLLNNEIDWLKRIRDDVKRTGETGVEETILEGHLGVTKELLAFQTPEKKFHIGCEKGGANLIKELIDDFIFPASNVYLQYMRNGELPAEQAIPVCGSPATINAGFELLVALAVGCVRNLKQIVDSLTEMYYIGTAITTCEALTEWEYLPPVGPRPPKGFVGLKNAGATCYMNSVIQQLYMIPSIRNGILAIEGTGSDVDDDMSGDEKQDNESNVDPRDDVFGYPQQFEDKPPLSKTEDRKEYNIGVLRHLQVIFGHLAASRLQYYVPRGFWKQFRLWGEPVNLREQHDALEFFNSLVDSLDEALKALGHPAMLSKVLGGSFADQKICQGCPHRYECEESFTTLNVDIRNHQNLLDSLEQYVKGDLLEGANAYHCEKCNKKVDTVKRLLIKKLPPVLAIQLKRFDYDWERECAIKFNDYFEFPRELDMEPYTVAGVAKLEGDNVNPESQLIQQNEQSESEKAGSTKYRLVGVLVHSGQASGGHYYSYIIQRNGGDGEKNRWYKFDDGDVTECKMDDDEEMKNQCFGGEYMGEVFDHMMKRMSYRRQKRWWNAYILFYERMDTIDHDDEVIRYISEIAITTRPHQIVMPSAIERSVRKQNVQFMHNRMQYSLEYFQFMKKLLTCNGVYLNPPPGQDHLSPEAEEITMISIQLAARFLFTTGFHTKKIVRGSASDWYDALCILLRHSKNVRFWFAHNVLFNVSNRFSEYLLECPSAEVRGAFAKLIVFIAHFSLQDGPCPSPFASPGPSSQAYDNLSLSDHLLRAVLNLLRREVSEHGRHLQQYFNLFVMYANLGVAEKTQLLKLSVPATFMLVSLDEGPGPPIKYQYAELGKLYSVVSQLIRCCNVSSRMQSSINGNPSLPNPFGDPNLSQPIMPIQQNVVDILFVRTSYVKKIIEDCSNSDETVKLLRFCCWENPQFSSTVLSELLWQVAYSYTYELRPYLDLLLQILLIEDSWQTHRIHNALKGIPDDRDGLFDTIQRSKNHYQKRAYQCIKCMVALFSSCPVAYQILQGNGDLKRKWTWAVEWLGDELERRPYTGNPQYTYNNWSPPVQSNETSNGYFLERSHSARMTLAKACELCPEEEPDDQDAPDEHESPPPEDAPLYPHSPGSQYQQNNHVHGQPYTGPAAHHMNNPQRTGQRAQENYEGSEEVSPPQTKDQ

Target ORF information:

RefSeq Version	XM_006256670.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ubiquitin specific peptidase 9, X-linked (Usp9x), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006256670.3

ORF Insert Sequence:

ATGACAGCCA CGACTCGTGG CTCTCCAGTT GGAGGGAATG ACAACCAGGG CCAAGCTCCT 
GATGGACAGT CTCAGCCCCC CCTCCAACAG AATCAGACTT CATCGCCTGA TTCATCCAAT 
GAAAATTCCC CTGCAACTCC TCCTGATGAG CAAGGCCAAG GTGATGCTCC TCCCCAGATT 
GAAGATGAGG AACCTGCATT TCCACACACT GACCTGGCAA AGTTAGATGA TATGATCAAC 
AGGCCTCGCT GGGTCGTTCC AGTTTTGCCG AAAGGGGAAT TAGAAGTGCT TTTAGAAGCT 
GCTATTGATC TTAGTAAGAA AGGCCTTGAT GTCAAAAGTG AAGCATGTCA GAGATTTTTC 
CGAGATGGGC TAACAATCTC ATTCACAAAA ATCCTTACAG ATGAAGCAGT GAGTGGCTGG 
AAGTTTGAAA TTCATAGATG TATTATTAAC AATACTCATC GCCTGGTGGA GCTGTGTGTG 
GCTAAGTTAG CCCAAGACTG GTTTCCACTT CTAGAACTTC TTGCCATGGC CTTAAATCCT 
CATTGCAAAT TCCATATCTA CAATGGTACA CGTCCCTGCG AGTCAGTGTC CTCAAGTGTT 
CAGTTGCCTG AAGATGAACT CTTTGCTCGT TCTCCAGACC CTCGATCACC AAAAGGTTGG 
CTAGTGGATC TCCTCAACAA ATTTGGCACT TTAAATGGAT TCCAGATACT GCATGATCGT 
TTTATTAATG GATCAGCATT AAATGTTCAG ATAATTGCAG CCCTTATTAA ACCATTTGGA 
CAGTGCTATG AGTTTCTTAC TCTTCACACG GTGAAAAAAT ACTTTCTTCC AATAATAGAA 
ATGGTTCCAC AGTTTTTAGA AAACTTAACT GATGAAGAGC TGAAGAAAGA AGCAAAGAAT 
GAAGCCAAAA ATGATGCTCT CTCAATGATT ATTAAATCAT TGAAGAGTTT AGCTTCACGA 
GTTCCTGGAC AGGAAGAAAC TGTAAAGAAC TTAGAAATAT TTAGGTTAAA AATGATACTT 
AGATTATTGC AAATTTCTTC CTTCAATGGA AAGATGAATG CACTGAATGA GGTTAATAAG 
GTGATTTCCA GTGTGTCATA CTATACCCAT CGGCATGGCA GTTCTGAGGA AGAAGAGTGG 
CTCACAGCAG AGAGGATGGC TGAATGGATA CAGCAGAACA ATATTTTATC AATAGTTTTA 
CGAGATAGTC TTCATCAACC ACAGTATGTA GAGAAACTAG AAAAGATTCT TCGCTTTGTC 
ATAAAAGAAA AAGCTCTGAC CTTGCAAGAT CTTGATAATA TCTGGGCAGC ACAGGCAGGG 
AAACATGAAG CCATCGTAAA GAATGTCCAT GATCTACTGG CCAAATTGGC ATGGGATTTT 
TCTCCTGAAC AACTTGATCA TCTTTTTGAT TGCTTTAAGG CCAGTTGGAC AAATGCAAGT 
AAAAAGCAAC GTGAAAAGCT CCTTGAGCTG ATTCGTCGTC TTGCAGAAGA TGATAAAGAT 
GGTGTGATGG CACATAAAGT GTTGAACCTT CTGTGGAATC TAGCTCACAG TGATGATGTG 
CCTGTGGATA TCATGGACTT GGCTCTCAGT GCTCACATCA AAATACTAGA TTATAGTTGC 
TCCCAGGACC GGGACACACA AAAGATCCAG TGGATAGATC GCTTCATAGA AGAACTTCGC 
ACAAATGACA AGTGGGTCAT TCCTGCACTG AAACAAATTA GAGAAATTTG CAGTTTGTTT 
GGTGAAGCAC CTCAAAATTT GAGTCAAACT CAGAGAAGTC CCCATGTATT TTATCGCCAT 
GATTTAATCA ATCAACTTCA GCACAATCAT GCCCTAGTTA CTTTGGTAGC AGAAAACCTT 
GCAACTTACA TGGAAAGCAT GAGACTGTAT GGCAGAGACA ATGAAGACTA TGACCCACAA 
ACTGTGAGAG TGGGAAGTAG ATATAGTCAT GTTCAAGAAG TCCAAGAACG TCTTAACTTT 
CTTAGATTTT TATTGAAGGA TGGCCAGCTA TGGCTGTGTG CTCCTCAGGC AAAACAAATA 
TGGAAGTGCT TAGCAGAGAA TGCGGTTTAT CTTTGTGACC GTGAAGCCTG CTTTAAGTGG 
TATTCCAAAT TAATGGGAGA TGAACCAGAC TTAGATCCTG ATATCAATAA GGACTTCTTT 
GAAAGTAATG TGCTTCAGCT TGATCCTTCA CTATTAACTG AAAATGGAAT GAAATGTTTT 
GAGAGATTCT TCAAAGCTGT GAATTGTCGA GAAGGAAAAC TAGTAGCAAA AAGAAGAGCC 
TATATGATGG ATGATTTGGA ATTGATAGGA TTAGACTATC TTTGGAGGGT TGTAATTCAG 
AGTAATGATG ATATTGCCAG CCGAGCTATT GATCTCCTCA AAGAGATATA CACAAACCTT 
GGTCCAAGAC TGCAGGTCAA TCAGGTGGTG ATCCATGAAG ACTTCATTCA GTCTTGCTTT 
GATCGTTTGA AAGCCTCTTA TGACACGTTG TGTGTTTTGG ATGGTGACAA AGACAGTATT 
AATTGTGCAA GACAGGAAGC TGTTCGAATG GTCCGAGTAT TAACTGTTTT AAGAGAATAT 
ATAAATGAAT GTGACAGTGA TTATCATGAA GAAAGAACAA TTTTGCCTAT GTCAAGAGCT 
TTCCGTGGTA AACACCTCTC TTTTATAGTT CGGTTTCCAA ACCAGGGCAG ACAAGTAGAT 
GACTTGGAAG TTTGGTCTCA TACAAATGAT ACCATTGGTT CAGTTCGACG ATGTATACTA 
AATCGCATTA AAGCCAATGT AGCTCATACA AAAATTGAAC TCTTTGTGGG TGGTGAGCTT 
ATTGATCCTG GAGATGACAG AAAGTTGATT GGACAATTAA ACCTAAAAGA TAAATCACTA 
ATTACAGCCA AACTTACACA AATCAGCTCC AATATGCCGT CAAGTCCTGA TAGCTCCTCT 
GATTCCTCAA CTGGATCTCC TGGAAACCAT GGTAATCATT ACAGTGATGG TCCCAACCCA 
GAGGTAGAAA GCTGTTTGCC TGGAGTGATA ATGTCACTTC ATCCCAGATA CATCTCTTTC 
CTTTGGCAAG TTGCAGACTT AGGGAGCAGC CTAAATATGC CCCCTCTTAG AGATGGAGCA 
AGAGTTCTTA TGAAACTTAT GCCGCCAGAT AGTACAACAA TAGAAAAATT AAGAGCTATT 
TGTTTAGATC ATGCCAAACT TGGAGAAAGC AGCCTTAGTC CATCTCTTGA CTCACTTTTC 
TTTGGTCCTT CAGCCTCACA AGTGCTATAC CTAACAGAGG TAGTCTATGC CCTGTTAATG 
CCTGCTGGTG CACCCCTGGC TGATGACTCC TCTGATTTTC AGTTTCACTT CTTGAAAAGT 
GGTGGTTTGC CCCTTGTTCT GAGTATGCTA ACCAGAAATA ACTTCCTACC AAATGCTGAT 
ATGGAAACTC GAAGGGGTGC CTACCTCAAT GCTCTTAAAA TAGCCAAACT GTTACTAACT 
GCCATTGGCT ATGGCCATGT TCGGGCTGTG GCAGAAGCAT GTCAGCCAGG TGTAGAAGGC 
GTGAATCCTA TGACATCGGT CAACCAAGTA ACTCATGATC AAGCAGTGGT GCTACAAAGT 
GCCCTTCAGA GCATTCCTAA CCCATCATCT GAATGCATGC TTAGAAACGT GTCTGTTCGT 
CTTGCTCAGC AGATTTCTGA TGAGGCTTCA AGATATATGC CTGATATTTG TGTAATTAGA 
GCTATACAAA AGATTATTTG GACATCAGGA TGTGGGGGAT TACAACTGGT ATTCAGCCCA 
AATGAGGAAG TCACAAAAAT TTATGAGAAG ACCAATGCAG GCAATGAGCC AGACCTTGAA 
GATGAACAGG TTTGCTGTGA GGCATTGGAA GTGATGACAC TGTGTTTCGC CCTGATTCCA 
ACAGCCTTAG ATGCTCTAAG TAAAGAAAAG GCTTGGCAGA CATTTATTAT TGACTTACTG 
TTACACTGTC ACAGCAAAAC TGTCCGTCAA GTAGCACAGG AACAATTCTT CTTAATGTGC 
ACCAGATGTT GCATGGGACA CAGGCCTCTA CTTTTCTTCA TTACTCTACT CTTTACTGTT 
TTGGGGAGCA CAGCCAGAGA GAGGGCTAAA CATTCAGGCG ACTACTTTAC TCTTTTAAGG 
CATCTTCTTA ATTACGCTTA CAACAGTAAC ATTAATGTAC CCAATGCTGA AGTTCTTCTA 
AATAATGAAA TTGATTGGCT TAAAAGAATT AGGGATGATG TTAAAAGAAC AGGTGAAACA 
GGCGTTGAAG AGACAATCTT AGAAGGACAC CTTGGGGTTA CAAAGGAGCT ACTGGCTTTT 
CAGACCCCTG AGAAAAAGTT TCATATTGGT TGTGAAAAGG GGGGTGCTAA TCTCATTAAA 
GAATTAATCG ACGATTTCAT CTTTCCTGCA TCCAATGTTT ACCTCCAGTA TATGAGAAAT 
GGAGAACTCC CTGCTGAGCA GGCCATTCCT GTCTGTGGTT CACCAGCCAC AATTAATGCT 
GGTTTTGAGT TACTTGTAGC ATTAGCTGTT GGCTGTGTAA GGAACCTCAA ACAGATAGTA 
GATTCTTTGA CTGAAATGTA TTACATTGGT ACAGCAATAA CTACTTGTGA GGCACTTACT 
GAGTGGGAAT ACCTGCCACC TGTTGGACCC CGTCCACCAA AAGGATTTGT GGGGCTGAAA 
AATGCTGGTG CTACTTGTTA CATGAATTCT GTGATTCAGC AACTCTATAT GATTCCCTCT 
ATCAGGAACG GTATTCTTGC AATTGAAGGC ACAGGTAGTG ATGTAGATGA TGATATGTCT 
GGGGATGAGA AGCAGGACAA TGAGAGCAAT GTTGATCCCA GGGATGATGT GTTTGGATAT 
CCTCAACAAT TTGAAGACAA ACCACCACTG AGTAAAACAG AAGATAGAAA AGAGTACAAT 
ATTGGTGTTC TAAGACACCT TCAAGTTATC TTTGGCCATT TAGCTGCTTC TCGACTACAG 
TACTATGTGC CCAGAGGATT TTGGAAACAG TTCAGGCTTT GGGGTGAGCC TGTTAATCTT 
CGTGAACAAC ATGATGCTTT AGAGTTCTTT AATTCATTGG TGGATAGTTT AGATGAAGCT 
TTAAAAGCCT TAGGGCATCC AGCTATGCTA AGTAAAGTCT TGGGAGGTTC CTTCGCTGAT 
CAGAAAATTT GTCAAGGCTG CCCACATAGG TATGAATGTG AAGAATCTTT TACGACTCTG 
AATGTAGACA TTAGAAACCA CCAAAATCTT CTTGATTCTT TGGAACAGTA TGTCAAAGGA 
GATTTACTAG AAGGGGCAAA TGCTTATCAT TGTGAAAAAT GCAATAAAAA GGTTGATACT 
GTAAAGCGCT TGCTAATTAA AAAGTTGCCT CCTGTTCTTG CTATCCAACT AAAACGATTC 
GATTATGACT GGGAAAGAGA ATGTGCAATC AAGTTCAATG ATTACTTTGA GTTTCCTCGA 
GAGTTAGACA TGGAGCCGTA CACAGTTGCA GGTGTTGCTA AACTAGAGGG AGATAATGTC 
AACCCAGAAA GTCAGCTGAT ACAACAGAAT GAGCAGTCTG AAAGCGAAAA AGCCGGAAGC 
ACAAAATACA GACTTGTGGG TGTGCTTGTA CACAGTGGTC AAGCAAGTGG GGGACATTAC 
TATTCTTACA TCATTCAGAG GAATGGAGGA GATGGTGAAA AAAATCGTTG GTATAAATTT 
GATGATGGAG ATGTAACAGA GTGTAAAATG GATGATGATG AAGAAATGAA AAACCAGTGT 
TTTGGTGGCG AGTACATGGG AGAAGTGTTT GATCACATGA TGAAGCGCAT GTCGTACAGG 
CGGCAGAAAA GGTGGTGGAA TGCATATATA CTTTTTTATG AAAGAATGGA TACGATAGAT 
CATGATGATG AGGTGATAAG ATACATATCA GAGATTGCTA TCACCACAAG GCCCCATCAG 
ATTGTTATGC CATCGGCCAT TGAAAGAAGT GTGCGGAAGC AGAATGTCCA ATTCATGCAT 
AACCGAATGC AGTACAGTTT GGAATATTTT CAGTTTATGA AAAAGCTACT TACATGTAAT 
GGTGTTTATT TAAACCCTCC ACCAGGGCAA GATCACCTGT CTCCTGAAGC AGAAGAAATC 
ACTATGATTA GTATTCAGCT TGCTGCTAGG TTCCTTTTTA CTACAGGATT TCACACAAAG 
AAAATAGTCC GAGGATCCGC CAGTGATTGG TATGATGCAT TGTGTATTCT CCTCCGTCAC 
AGCAAGAATG TGCGGTTTTG GTTTGCTCAC AATGTGCTTT TTAATGTTTC AAATCGATTC 
TCTGAGTACC TTTTGGAATG CCCTAGTGCA GAAGTGAGAG GTGCATTTGC TAAACTTATA 
GTTTTCATTG CACACTTTTC CTTACAAGAT GGGCCTTGTC CTTCACCTTT TGCATCTCCT 
GGACCTTCTA GTCAGGCTTA TGATAACTTA AGTTTGAGTG ACCACCTACT AAGAGCAGTA 
CTAAATCTCT TGAGGAGGGA GGTTTCAGAG CATGGACGTC ATTTACAGCA GTATTTCAAC 
TTGTTTGTAA TGTATGCCAA TTTAGGAGTG GCAGAGAAAA CACAGCTGCT CAAACTGAGT 
GTACCTGCTA CCTTTATGCT TGTGTCCTTA GATGAAGGTC CTGGTCCTCC AATTAAATAT 
CAGTATGCTG AATTAGGCAA ATTATACTCC GTAGTGTCAC AGTTAATCCG CTGTTGCAAT 
GTCTCTTCAA GAATGCAGTC TTCAATCAAT GGTAATCCTT CTCTTCCAAA TCCTTTTGGT 
GATCCTAATT TATCACAACC TATAATGCCA ATTCAACAAA ATGTGGTAGA CATTTTATTT 
GTGAGAACAA GTTATGTGAA GAAAATTATT GAAGACTGCA GTAACTCTGA TGAGACCGTC 
AAATTGCTTC GCTTTTGCTG CTGGGAGAAT CCTCAGTTCT CATCTACTGT CCTGAGTGAA 
CTTCTTTGGC AGGTTGCGTA TTCCTATACT TACGAACTCC GGCCCTATTT GGATCTGCTT 
TTACAAATCT TACTGATTGA AGACTCCTGG CAGACTCACA GAATTCATAA TGCCCTTAAA 
GGAATTCCAG ATGACCGAGA TGGGCTGTTT GACACAATTC AGCGTTCTAA GAATCACTAT 
CAAAAAAGAG CATACCAATG TATAAAATGT ATGGTAGCCC TCTTTAGCAG TTGTCCTGTG 
GCTTACCAAA TCCTGCAGGG CAATGGAGAT CTTAAAAGGA AATGGACCTG GGCAGTAGAG 
TGGCTTGGAG ATGAACTCGA AAGAAGACCA TACACTGGCA ATCCTCAGTA CACTTACAAC 
AACTGGTCTC CTCCAGTGCA AAGCAATGAA ACATCAAATG GCTACTTCTT GGAGAGATCA 
CATAGCGCTA GGATGACACT TGCAAAAGCT TGTGAACTCT GTCCTGAGGA GGAACCAGAT 
GACCAAGATG CCCCAGATGA GCATGAATCA CCTCCACCTG AAGATGCCCC ATTATACCCC 
CATTCCCCTG GATCTCAATA TCAGCAGAAT AACCATGTGC ATGGACAGCC CTATACAGGC 
CCAGCCGCAC ATCACATGAA CAACCCTCAG AGAACTGGCC AACGAGCACA AGAAAATTAT 
GAAGGCAGTG AAGAAGTGTC CCCACCTCAG ACCAAGGATC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Deubiquitinating enzyme USP9X regulates cellular clock function by modulating the ubiquitination and degradation of a core circadian protein BMAL1.
The Biochemical journal475(8)1507-1522(2018 04)
Zhang Y,Duan C,Yang J,Chen S,Liu Q,Zhou L,Huang Z,Xu Y,Xu G

Noncanonical regulation of alkylation damage resistance by the OTUD4 deubiquitinase.
The EMBO journal34(12)1687-703(2015 Jun)
Zhao Y,Majid MC,Soll JM,Brickner JR,Dango S,Mosammaparast N

Mutations in USP9X are associated with X-linked intellectual disability and disrupt neuronal cell migration and growth.
American journal of human genetics94(3)470-8(2014 Mar)
Homan CC,Kumar R,Nguyen LS,Haan E,Raymond FL,Abidi F,Raynaud M,Schwartz CE,Wood SA,Gecz J,Jolly LA

Loss of Usp9x disrupts cortical architecture, hippocampal development and TGFβ-mediated axonogenesis.
PloS one8(7)e68287(2013)
Stegeman S,Jolly LA,Premarathne S,Gecz J,Richards LJ,Mackay-Sim A,Wood SA

Identification of ubiquitin-specific protease 9X (USP9X) as a deubiquitinase acting on ubiquitin-peroxin 5 (PEX5) thioester conjugate.
The Journal of biological chemistry287(16)12815-27(2012 Apr)
Grou CP,Francisco T,Rodrigues TA,Freitas MO,Pinto MP,Carvalho AF,Domingues P,Wood SA,Rodríguez-Borges JE,Sá-Miranda C,Fransen M,Azevedo JE

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Usp9x cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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