Ndc1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ndc1
Entrez Gene ID	362557
Full Name	NDC1 transmembrane nucleoporin
Synonyms	Tmem48
General protein information	Preferred Names NDC1 transmembrane nucleoporin Names nucleoporin NDC1 transmembrane protein 48
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 5 Map Location: 5q34

mRNA and Protein(s)

mRNA	Protein	Name
XM_017593491.1	XP_017448980.1	nucleoporin NDC1 isoform X1
NM_001025023.1	NP_001020194.1	nucleoporin NDC1

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	TMEM48	XP_001235442.3
Pan troglodytes (chimpanzee)	TMEM48	XP_513417.4
Drosophila melanogaster (fruit fly)	CG5857	NP_651191.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004558	XP_313856.5
Homo sapiens (human)	NDC1	NP_060557.3
Xenopus tropicalis (tropical clawed frog)	ndc1	NP_001016813.1
Bos taurus (cattle)	NDC1	XP_003585949.2
Mus musculus (house mouse)	Ndc1	NP_082631.1
Rattus norvegicus (Norway rat)	Ndc1	NP_001020194.1
Danio rerio (zebrafish)	ndc1	NP_956237.1
Canis lupus familiaris (dog)	NDC1	XP_003434706.1

Gene Ontology
Bibliography

Related articles in PubMed

A mutation in the nuclear pore complex gene Tmem48 causes gametogenesis defects in skeletal fusions with sterility (sks) mice.
Akiyama K, Noguchi J, Hirose M, Kajita S, Katayama K, Khalaj M, Tsuji T, Fairfield H, Byers C, Reinholdt L, Ogura A, Kunieda T
The Journal of biological chemistry288(44)31830-41(2013 Nov)

Defining the membrane proteome of NK cells.
Ghosh D, Lippert D, Krokhin O, Cortens JP, Wilkins JA
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)

The conserved transmembrane nucleoporin NDC1 is required for nuclear pore complex assembly in vertebrate cells.
Mansfeld J, Güttinger S, Hawryluk-Gara LA, Panté N, Mall M, Galy V, Haselmann U, Mühlhäusser P, Wozniak RW, Mattaj IW, Kutay U, Antonin W
Molecular cell22(1)93-103(2006 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ndc1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ndc1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa07056	NM_001025023.1 Latest version!	Rattus norvegicus NDC1 transmembrane nucleoporin (Ndc1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ORa61303	XM_017593491.1 Latest version!	Rattus norvegicus NDC1 transmembrane nucleoporin (Ndc1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ndc1 ( NM_001025023.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001020194.1 Rattus norvegicus NDC1 transmembrane nucleoporin (Ndc1), mRNA.

Price & Availability↑

CloneID	ORa07056
Clone ID Related Accession (Same CDS sequence)	NM_001025023.1
Accession Version	NM_001025023.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2022bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577203200000
Organism	Rattus norvegicus(Norway rat)
Product	nucleoporin NDC1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC079443.1. On Jun 25, 2005 this sequence version replaced XM_575879.1. ##Evidence-Data-START## Transcript exon combination :: BC079443.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCCACGG CTGCGAGCGG GCCCTGCGCC GGCGGGTCAC CGCGGGACAT CCTTTGGCGC 
GTTTTGGGCT GGAGGATAGT TACTAGTGTC GTTTGGTCAG TGCTGCTTCT CCCTGTCTGC 
ATCACAGCAT TTATAGTCTT CAGCAGCATC GATTTATTCC ACCCTATACA GTGGCTGTCA 
GATTCTCTCA ATGAGTTCTA TAGTTCCCAA GTAATCTTTT ACCTCCTGCT GCTGGCCGTG 
GTCATTATAA TAATAAGTAT TTTCAATGTG GAGTTCTATA CAGTTGTGCC CTCCATACCC 
GGCTCCCGAC TGGCGCTGAT AGCACGGATC CTTCACCCTC AGCAGCTCAC GCACTCCTTC 
ATACACGCTG TGATGGGAAT GGCAGTGGCT TGGTGTGCGG CCATCATGAC CAAGGGCCAG 
TCCAGGTCTC TGGTAGTTCC CTGCACTGGA ACGGAGAGCT TAGATAGCCC TGCTGCTCAA 
ACCTGCATGA ATGAATATCA TCTGTTTTTC CTACTGTCTG GAGCATTCAT GGGCTATAGC 
TATAGTCTTC TGTATTTCAT TAACAACATG AATTATCTTC CGTTTCCTAT TATACAGCAG 
TACAAATTTT TGCGTTTCCG GAGATCTCTC CTCTTACTAG TTAAACATAG CTGTGTGGAA 
TCCCTGTTCA TGGTCAGAAA TTTCTGCATT GTATATTATT TTTTTGGTCA TATTCCCAAA 
GCATGGATTA GCACTGCTCT GGACCTTCGC ACAGACGAGC AGGCCCACAG GCCTCTTGAC 
ACGATAGGCG GTCTCCTGAA CGTCTCATTG CTCTACCATG TCTGGCTGTG TGGTGTCTTT 
CTCTTGATGA CTTGGTATAT CTCATGGATA CTCTTCAAAA TCTATGCCAC AGAGGCTTAT 
ATGTTCCCTG TTCAGCCACC ATTTGCGGAA GAATCAGATG AATGCCTTCC TAAAGTCTTA 
AATAGCAATC CCCCACTCAT CATAAAGTAT TTGGCCTTAC AGGACCTGAT GTTGCTTTCT 
CAGTATTCTC CTTCTCGAAG GCAAGAAGTT TTCAGCCTCA GCCAACCAGG TGGACATCCC 
CACAATTGGA CTGCTATTTC GAGGGAGTGT TTGAGTCTTT TAAATGATAT GACTCAGAAG 
CTGGTTCTCT ACCAAGAAGC TGCTGCTACG AATGGGCGGA TGTATTCCTC ATACTCAGTG 
GAACCCAAGA GACTGAGTTC TCCAGAAGAA ACTGCTTTTC AGACTCCAAA ACCTAGCCAG 
ATGCCTTCCG TGCCGCCATT AGTTAAAACC TCATTGTTTT CTCCAAAATT AAGTACACCT 
GACGGTTCAA GTCCTTTTGG GACTCCGTTT GGCTCCAGTG TAGTGAACCG AATGGCTGGA 
ATTTTTGATG TAAACACCTG CTGTGGATCT CCCCAGAGTC CTCAGCTTGT CAGAAGAGGG 
CCGAGACTGT GGACTCACAC TTCTGATCAG CAAGTGTCTG CAGTCTCTAA TCCCTCTCCT 
TGTGCCTCTG TCACTGCTGA GGGCAAGACA GTGAGGCAGC CCAGTGTGCT CTACTCGTGG 
ATTCAGAATA AACGGGAGCA GATTAAGAAT TTCTTGTCGA AACGGGTGCT GATAATGTAC 
TTTTTCAGTA AGCATCCAGA GGCCTCCATT CAGGCTGTTT TTTCAGATGC CCAAATGCAT 
ATTTGGGCTT TAGAAGGTCT GTCTCACTTA GTAGCAGCAT CATTTACAGA AGATAGATTT 
GGAGTTGTCC AGACCACGCT CCCAGCTATC CTTAATACTT TGCTGACTCT GCAGGAGGCT 
GTTGACAAAT ACTTCAAGCT GCCCCATGCT TCTAGTAAAC CACCCCGAAC TTCAGGAAGC 
CTCGTGGACA CATCCTACAA AACATTAAGA TTTGCTTTCA GGGCCTCACT GAAAACTGCT 
ATCTACCGTA TAACCACCAC GTTTGGTGAA CATCTGAATG CTGTGCAAGC ATCTGCAGAA 
CATCAGAAAA GACTTCAACA GTTCTTGGAG TTCAAAGAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001020194.1
CDS	339..2360
Translation	MATAASGPCAGGSPRDILWRVLGWRIVTSVVWSVLLLPVCITAFIVFSSIDLFHPIQWLSDSLNEFYSSQVIFYLLLLAVVIIIISIFNVEFYTVVPSIPGSRLALIARILHPQQLTHSFIHAVMGMAVAWCAAIMTKGQSRSLVVPCTGTESLDSPAAQTCMNEYHLFFLLSGAFMGYSYSLLYFINNMNYLPFPIIQQYKFLRFRRSLLLLVKHSCVESLFMVRNFCIVYYFFGHIPKAWISTALDLRTDEQAHRPLDTIGGLLNVSLLYHVWLCGVFLLMTWYISWILFKIYATEAYMFPVQPPFAEESDECLPKVLNSNPPLIIKYLALQDLMLLSQYSPSRRQEVFSLSQPGGHPHNWTAISRECLSLLNDMTQKLVLYQEAAATNGRMYSSYSVEPKRLSSPEETAFQTPKPSQMPSVPPLVKTSLFSPKLSTPDGSSPFGTPFGSSVVNRMAGIFDVNTCCGSPQSPQLVRRGPRLWTHTSDQQVSAVSNPSPCASVTAEGKTVRQPSVLYSWIQNKREQIKNFLSKRVLIMYFFSKHPEASIQAVFSDAQMHIWALEGLSHLVAASFTEDRFGVVQTTLPAILNTLLTLQEAVDKYFKLPHASSKPPRTSGSLVDTSYKTLRFAFRASLKTAIYRITTTFGEHLNAVQASAEHQKRLQQFLEFKE

Target ORF information:

RefSeq Version	NM_001025023.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus NDC1 transmembrane nucleoporin (Ndc1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001025023.1

ORF Insert Sequence:

ATGGCCACGG CTGCGAGCGG GCCCTGCGCC GGCGGGTCAC CGCGGGACAT CCTTTGGCGC 
GTTTTGGGCT GGAGGATAGT TACTAGTGTC GTTTGGTCAG TGCTGCTTCT CCCTGTCTGC 
ATCACAGCAT TTATAGTCTT CAGCAGCATC GATTTATTCC ACCCTATACA GTGGCTGTCA 
GATTCTCTCA ATGAGTTCTA TAGTTCCCAA GTAATCTTTT ACCTCCTGCT GCTGGCCGTG 
GTCATTATAA TAATAAGTAT TTTCAATGTG GAGTTCTATA CAGTTGTGCC CTCCATACCC 
GGCTCCCGAC TGGCGCTGAT AGCACGGATC CTTCACCCTC AGCAGCTCAC GCACTCCTTC 
ATACACGCTG TGATGGGAAT GGCAGTGGCT TGGTGTGCGG CCATCATGAC CAAGGGCCAG 
TCCAGGTCTC TGGTAGTTCC CTGCACTGGA ACGGAGAGCT TAGATAGCCC TGCTGCTCAA 
ACCTGCATGA ATGAATATCA TCTGTTTTTC CTACTGTCTG GAGCATTCAT GGGCTATAGC 
TATAGTCTTC TGTATTTCAT TAACAACATG AATTATCTTC CGTTTCCTAT TATACAGCAG 
TACAAATTTT TGCGTTTCCG GAGATCTCTC CTCTTACTAG TTAAACATAG CTGTGTGGAA 
TCCCTGTTCA TGGTCAGAAA TTTCTGCATT GTATATTATT TTTTTGGTCA TATTCCCAAA 
GCATGGATTA GCACTGCTCT GGACCTTCGC ACAGACGAGC AGGCCCACAG GCCTCTTGAC 
ACGATAGGCG GTCTCCTGAA CGTCTCATTG CTCTACCATG TCTGGCTGTG TGGTGTCTTT 
CTCTTGATGA CTTGGTATAT CTCATGGATA CTCTTCAAAA TCTATGCCAC AGAGGCTTAT 
ATGTTCCCTG TTCAGCCACC ATTTGCGGAA GAATCAGATG AATGCCTTCC TAAAGTCTTA 
AATAGCAATC CCCCACTCAT CATAAAGTAT TTGGCCTTAC AGGACCTGAT GTTGCTTTCT 
CAGTATTCTC CTTCTCGAAG GCAAGAAGTT TTCAGCCTCA GCCAACCAGG TGGACATCCC 
CACAATTGGA CTGCTATTTC GAGGGAGTGT TTGAGTCTTT TAAATGATAT GACTCAGAAG 
CTGGTTCTCT ACCAAGAAGC TGCTGCTACG AATGGGCGGA TGTATTCCTC ATACTCAGTG 
GAACCCAAGA GACTGAGTTC TCCAGAAGAA ACTGCTTTTC AGACTCCAAA ACCTAGCCAG 
ATGCCTTCCG TGCCGCCATT AGTTAAAACC TCATTGTTTT CTCCAAAATT AAGTACACCT 
GACGGTTCAA GTCCTTTTGG GACTCCGTTT GGCTCCAGTG TAGTGAACCG AATGGCTGGA 
ATTTTTGATG TAAACACCTG CTGTGGATCT CCCCAGAGTC CTCAGCTTGT CAGAAGAGGG 
CCGAGACTGT GGACTCACAC TTCTGATCAG CAAGTGTCTG CAGTCTCTAA TCCCTCTCCT 
TGTGCCTCTG TCACTGCTGA GGGCAAGACA GTGAGGCAGC CCAGTGTGCT CTACTCGTGG 
ATTCAGAATA AACGGGAGCA GATTAAGAAT TTCTTGTCGA AACGGGTGCT GATAATGTAC 
TTTTTCAGTA AGCATCCAGA GGCCTCCATT CAGGCTGTTT TTTCAGATGC CCAAATGCAT 
ATTTGGGCTT TAGAAGGTCT GTCTCACTTA GTAGCAGCAT CATTTACAGA AGATAGATTT 
GGAGTTGTCC AGACCACGCT CCCAGCTATC CTTAATACTT TGCTGACTCT GCAGGAGGCT 
GTTGACAAAT ACTTCAAGCT GCCCCATGCT TCTAGTAAAC CACCCCGAAC TTCAGGAAGC 
CTCGTGGACA CATCCTACAA AACATTAAGA TTTGCTTTCA GGGCCTCACT GAAAACTGCT 
ATCTACCGTA TAACCACCAC GTTTGGTGAA CATCTGAATG CTGTGCAAGC ATCTGCAGAA 
CATCAGAAAA GACTTCAACA GTTCTTGGAG TTCAAAGAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ndc1 ( XM_017593491.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017448980.1 Rattus norvegicus NDC1 transmembrane nucleoporin (Ndc1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa61303
Clone ID Related Accession (Same CDS sequence)	XM_017593491.1
Accession Version	XM_017593491.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2091bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	nucleoporin NDC1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCACC GTCGCCCTTT GTTTTTGACG CCCCGATGGA CCCTGCTTCG GAAGGATTTT 
TTGGACTCCA GAGGACGGTT CTTCGAGTCA CAGCCCACCT GGAGGCATGT GGCTACGGGG 
AAGTTCTGGC CGGTTGAGTC TGTTCAGGCG AAGCTGCGGA GAGATGGCCT GGCGGTTTTG 
CAGGACTCTG TCCAGGCAGC GTTCCGGGCA GCCCCAGCTG GGTCCTCCTG GGGCCTTCCT 
GCCTTTGAAC AGTGGTGGCG GGCGGGCACT CTGTCCCCAG CACGCTTGAG AGCGGGGGCC 
GCCATCTCCA TGGCCACGGC TGCGAGCGGG CCCTGCGCCG GCGGGTCACC GCGGGACATC 
CTTTGGCGCG TTTTGGGCTG GAGGATAGTT ACTAGTGTCG TTTGGTCAGT GCTGCTTCTC 
CCTGTCTGCA TCACAGCATT TATAGTCTTC AGCAGCATCG ATTTATTCCA CCCTATACAG 
TGGCTGTCAG ATTCTCTCAA TGAGTTCTAT AGTTCCCAAG TAATCTTTTA CCTCCTGCTG 
CTGGCCGTGG TCATTATAAT AATAAGTATT TTCAATGTGG AGTTCTATAC AGTTGTGCCC 
TCCATACCCG GCTCCCGACT GGCGCTGATA GCACGGATCC TTCACCCTCA GCAGCTCACG 
CACTCCTTCA TACACGCTGT GATGGGAATG GCAGTGGCTT GGTGTGCGGC CATCATGACC 
AAGGGCCAGT CCAGGTCTCT GGTAGTTCCC TGCACTGGAA CGGAGAGCTT AGATAGCCCT 
GCTGCTCAAA CCTGCATGAA TGAATATCAT CTGTTTTTCC TACTGTCTGG AGCATTCATG 
GGCTATAGCT ATAGTCTTCT GTATTTCATT AACAACATGA ATTATCTTCC GTTTCCTATT 
ATACAGCAGT ACAAATTTTT GCGTTTCCGG AGATCTCTCC TCTTACTAGT TAAACATAGC 
TGTGTGGAAT CCCTGTTCAT GGTCAGAAAT TTCTGCATTG TATATTATTT TTTTGGTCAT 
ATTCCCAAAG CATGGATTAG CACTGCTCTG GACCTTCGCA CAGACGAGCA GGCCCACAGG 
CCTCTTGACA CGATAGGCGG TCTCCTGAAC GTCTCATTGC TCTACCATGT CTGGCTGTGT 
GGTGTCTTTC TCTTGATGAC TTGGTATATC TCATGGATAC TCTTCAAAAT CTATGCCACA 
GAGGCTTATA TGTTCCCTGT TCAGCCACCA TTTGCGGAAG AATCAGATGA ATGCCTTCCT 
AAAGTCTTAA ATAGCAATCC CCCACTCATC ATAAAGTATT TGGCCTTACA GGACCTGATG 
TTGCTTTCTC AGTATTCTCC TTCTCGAAGG CAAGAAGTTT TCAGCCTCAG CCAACCAGGT 
GGACATCCCC ACAATTGGAC TGCTATTTCG AGGGAGTGTT TGAGTCTTTT AAATGATATG 
ACTCAGAAGC TGGTTCTCTA CCAAGAAGCT GCTGCTACGA ATGGGCGGAT GTATTCCTCA 
TACTCAGTGG AACCCAAGAG ACTGAGTTCT CCAGATCAGC AAGTGTCTGC AGTCTCTAAT 
CCCTCTCCTT GTGCCTCTGT CACTGCTGAG GGCAAGACAG TGAGGCAGCC CAGTGTGCTC 
TACTCGTGGA TTCAGAATAA ACGGGAGCAG ATTAAGAATT TCTTGTCGAA ACGGGTGCTG 
ATAATGTACT TTTTCAGTAA GCATCCAGAG GCCTCCATTC AGGCTGTTTT TTCAGATGCC 
CAAATGCATA TTTGGGCTTT AGAAGGTCTG TCTCACTTAG TAGCAGCATC ATTTACAGAA 
GATAGATTTG GAGTTGTCCA GACCACGCTC CCAGCTATCC TTAATACTTT GCTGACTCTG 
CAGGAGGCTG TTGACAAATA CTTCAAGCTG CCCCATGCTT CTAGTAAACC ACCCCGAACT 
TCAGGAAGCC TCGTGGACAC ATCCTACAAA ACATTAAGAT TTGCTTTCAG GGCCTCACTG 
AAAACTGCTA TCTACCGTAT AACCACCACG TTTGGTGAAC ATCTGAATGC TGTGCAAGCA 
TCTGCAGAAC ATCAGAAAAG ACTTCAACAG TTCTTGGAGT TCAAAGAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017448980.1
CDS	34..2124
Translation	MQHRRPLFLTPRWTLLRKDFLDSRGRFFESQPTWRHVATGKFWPVESVQAKLRRDGLAVLQDSVQAAFRAAPAGSSWGLPAFEQWWRAGTLSPARLRAGAAISMATAASGPCAGGSPRDILWRVLGWRIVTSVVWSVLLLPVCITAFIVFSSIDLFHPIQWLSDSLNEFYSSQVIFYLLLLAVVIIIISIFNVEFYTVVPSIPGSRLALIARILHPQQLTHSFIHAVMGMAVAWCAAIMTKGQSRSLVVPCTGTESLDSPAAQTCMNEYHLFFLLSGAFMGYSYSLLYFINNMNYLPFPIIQQYKFLRFRRSLLLLVKHSCVESLFMVRNFCIVYYFFGHIPKAWISTALDLRTDEQAHRPLDTIGGLLNVSLLYHVWLCGVFLLMTWYISWILFKIYATEAYMFPVQPPFAEESDECLPKVLNSNPPLIIKYLALQDLMLLSQYSPSRRQEVFSLSQPGGHPHNWTAISRECLSLLNDMTQKLVLYQEAAATNGRMYSSYSVEPKRLSSPDQQVSAVSNPSPCASVTAEGKTVRQPSVLYSWIQNKREQIKNFLSKRVLIMYFFSKHPEASIQAVFSDAQMHIWALEGLSHLVAASFTEDRFGVVQTTLPAILNTLLTLQEAVDKYFKLPHASSKPPRTSGSLVDTSYKTLRFAFRASLKTAIYRITTTFGEHLNAVQASAEHQKRLQQFLEFKE

Target ORF information:

RefSeq Version	XM_017593491.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus NDC1 transmembrane nucleoporin (Ndc1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017593491.1

ORF Insert Sequence:

ATGCAGCACC GTCGCCCTTT GTTTTTGACG CCCCGATGGA CCCTGCTTCG GAAGGATTTT 
TTGGACTCCA GAGGACGGTT CTTCGAGTCA CAGCCCACCT GGAGGCATGT GGCTACGGGG 
AAGTTCTGGC CGGTTGAGTC TGTTCAGGCG AAGCTGCGGA GAGATGGCCT GGCGGTTTTG 
CAGGACTCTG TCCAGGCAGC GTTCCGGGCA GCCCCAGCTG GGTCCTCCTG GGGCCTTCCT 
GCCTTTGAAC AGTGGTGGCG GGCGGGCACT CTGTCCCCAG CACGCTTGAG AGCGGGGGCC 
GCCATCTCCA TGGCCACGGC TGCGAGCGGG CCCTGCGCCG GCGGGTCACC GCGGGACATC 
CTTTGGCGCG TTTTGGGCTG GAGGATAGTT ACTAGTGTCG TTTGGTCAGT GCTGCTTCTC 
CCTGTCTGCA TCACAGCATT TATAGTCTTC AGCAGCATCG ATTTATTCCA CCCTATACAG 
TGGCTGTCAG ATTCTCTCAA TGAGTTCTAT AGTTCCCAAG TAATCTTTTA CCTCCTGCTG 
CTGGCCGTGG TCATTATAAT AATAAGTATT TTCAATGTGG AGTTCTATAC AGTTGTGCCC 
TCCATACCCG GCTCCCGACT GGCGCTGATA GCACGGATCC TTCACCCTCA GCAGCTCACG 
CACTCCTTCA TACACGCTGT GATGGGAATG GCAGTGGCTT GGTGTGCGGC CATCATGACC 
AAGGGCCAGT CCAGGTCTCT GGTAGTTCCC TGCACTGGAA CGGAGAGCTT AGATAGCCCT 
GCTGCTCAAA CCTGCATGAA TGAATATCAT CTGTTTTTCC TACTGTCTGG AGCATTCATG 
GGCTATAGCT ATAGTCTTCT GTATTTCATT AACAACATGA ATTATCTTCC GTTTCCTATT 
ATACAGCAGT ACAAATTTTT GCGTTTCCGG AGATCTCTCC TCTTACTAGT TAAACATAGC 
TGTGTGGAAT CCCTGTTCAT GGTCAGAAAT TTCTGCATTG TATATTATTT TTTTGGTCAT 
ATTCCCAAAG CATGGATTAG CACTGCTCTG GACCTTCGCA CAGACGAGCA GGCCCACAGG 
CCTCTTGACA CGATAGGCGG TCTCCTGAAC GTCTCATTGC TCTACCATGT CTGGCTGTGT 
GGTGTCTTTC TCTTGATGAC TTGGTATATC TCATGGATAC TCTTCAAAAT CTATGCCACA 
GAGGCTTATA TGTTCCCTGT TCAGCCACCA TTTGCGGAAG AATCAGATGA ATGCCTTCCT 
AAAGTCTTAA ATAGCAATCC CCCACTCATC ATAAAGTATT TGGCCTTACA GGACCTGATG 
TTGCTTTCTC AGTATTCTCC TTCTCGAAGG CAAGAAGTTT TCAGCCTCAG CCAACCAGGT 
GGACATCCCC ACAATTGGAC TGCTATTTCG AGGGAGTGTT TGAGTCTTTT AAATGATATG 
ACTCAGAAGC TGGTTCTCTA CCAAGAAGCT GCTGCTACGA ATGGGCGGAT GTATTCCTCA 
TACTCAGTGG AACCCAAGAG ACTGAGTTCT CCAGATCAGC AAGTGTCTGC AGTCTCTAAT 
CCCTCTCCTT GTGCCTCTGT CACTGCTGAG GGCAAGACAG TGAGGCAGCC CAGTGTGCTC 
TACTCGTGGA TTCAGAATAA ACGGGAGCAG ATTAAGAATT TCTTGTCGAA ACGGGTGCTG 
ATAATGTACT TTTTCAGTAA GCATCCAGAG GCCTCCATTC AGGCTGTTTT TTCAGATGCC 
CAAATGCATA TTTGGGCTTT AGAAGGTCTG TCTCACTTAG TAGCAGCATC ATTTACAGAA 
GATAGATTTG GAGTTGTCCA GACCACGCTC CCAGCTATCC TTAATACTTT GCTGACTCTG 
CAGGAGGCTG TTGACAAATA CTTCAAGCTG CCCCATGCTT CTAGTAAACC ACCCCGAACT 
TCAGGAAGCC TCGTGGACAC ATCCTACAAA ACATTAAGAT TTGCTTTCAG GGCCTCACTG 
AAAACTGCTA TCTACCGTAT AACCACCACG TTTGGTGAAC ATCTGAATGC TGTGCAAGCA 
TCTGCAGAAC ATCAGAAAAG ACTTCAACAG TTCTTGGAGT TCAAAGAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A mutation in the nuclear pore complex gene Tmem48 causes gametogenesis defects in skeletal fusions with sterility (sks) mice.
The Journal of biological chemistry288(44)31830-41(2013 Nov)
Akiyama K,Noguchi J,Hirose M,Kajita S,Katayama K,Khalaj M,Tsuji T,Fairfield H,Byers C,Reinholdt L,Ogura A,Kunieda T

Defining the membrane proteome of NK cells.
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)
Ghosh D,Lippert D,Krokhin O,Cortens JP,Wilkins JA

The conserved transmembrane nucleoporin NDC1 is required for nuclear pore complex assembly in vertebrate cells.
Molecular cell22(1)93-103(2006 Apr)
Mansfeld J,Güttinger S,Hawryluk-Gara LA,Panté N,Mall M,Galy V,Haselmann U,Mühlhäusser P,Wozniak RW,Mattaj IW,Kutay U,Antonin W

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Ndc1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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