Ccar1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ccar1
Entrez Gene ID	361849
Full Name	cell division cycle and apoptosis regulator 1
Synonyms	RGD1560358
General protein information	Preferred Names cell division cycle and apoptosis regulator 1 Names cell division cycle and apoptosis regulator protein 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 20 Map Location: 20q11

mRNA and Protein(s)

mRNA	Protein	Name
XM_006256462.2	XP_006256524.1	cell division cycle and apoptosis regulator protein 1 isoform X1
NM_001108535.1	NP_001102005.1	cell division cycle and apoptosis regulator protein 1
XM_006256464.3	XP_006256526.1	cell division cycle and apoptosis regulator protein 1 isoform X2
XM_008772894.1	XP_008771116.1	cell division cycle and apoptosis regulator protein 1 isoform X1
XM_008772897.2	XP_008771119.1	cell division cycle and apoptosis regulator protein 1 isoform X3
XM_006256463.2	XP_006256525.1	cell division cycle and apoptosis regulator protein 1 isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	CCAR1	XP_536372.2
Bos taurus (cattle)	CCAR1	XP_005226582.1
Gallus gallus (chicken)	CCAR1	XP_004942066.1
Danio rerio (zebrafish)	LOC568087	NP_001139071.1
Xenopus tropicalis (tropical clawed frog)	ccar1	NP_001135676.1
Macaca mulatta (Rhesus monkey)	CCAR1	XP_001083295.1
Rattus norvegicus (Norway rat)	Ccar1	NP_001102005.1
Pan troglodytes (chimpanzee)	CCAR1	XP_001168607.1
Mus musculus (house mouse)	Ccar1	NP_080477.1
Homo sapiens (human)	CCAR1	NP_060707.2

Gene Ontology
Bibliography

Related articles in PubMed

CCAR1/CoCoA pair-mediated recruitment of the Mediator defines a novel pathway for GATA1 function.
Mizuta S, Minami T, Fujita H, Kaminaga C, Matsui K, Ishino R, Fujita A, Oda K, Kawai A, Hasegawa N, Urahama N, Roeder RG, Ito M
Genes to cells : devoted to molecular & cellular mechanisms19(1)28-51(2014 Jan)

CCAR1 promotes chromatin loading of androgen receptor (AR) transcription complex by stabilizing the association between AR and GATA2.
Seo WY, Jeong BC, Yu EJ, Kim HJ, Kim SH, Lim JE, Kwon GY, Lee HM, Kim JH
Nucleic acids research41(18)8526-36(2013 Oct)

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanhäusser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

CCAR1, a key regulator of mediator complex recruitment to nuclear receptor transcription complexes.
Kim JH, Yang CK, Heo K, Roeder RG, An W, Stallcup MR
Molecular cell31(4)510-9(2008 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ccar1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ccar1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa48033	XM_008772897.2 Latest version!	Rattus norvegicus cell division cycle and apoptosis regulator 1 (Ccar1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ORa07722	NM_001108535.1 Latest version!	Rattus norvegicus cell division cycle and apoptosis regulator 1 (Ccar1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa39466	XM_006256463.2 Latest version!	Rattus norvegicus cell division cycle and apoptosis regulator 1 (Ccar1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa39466	XM_008772894.1 Latest version!	Rattus norvegicus cell division cycle and apoptosis regulator 1 (Ccar1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa39466	XM_006256462.2 Latest version!	Rattus norvegicus cell division cycle and apoptosis regulator 1 (Ccar1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa39467	XM_006256464.3 Latest version!	Rattus norvegicus cell division cycle and apoptosis regulator 1 (Ccar1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ccar1 ( XM_008772897.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008771119.1 Rattus norvegicus cell division cycle and apoptosis regulator 1 (Ccar1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ORa48033
Clone ID Related Accession (Same CDS sequence)	XM_008772897.2
Accession Version	XM_008772897.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2898bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	cell division cycle and apoptosis regulator protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005119.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008772897.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTTTA AATGGAATGC ACAAAGAATT CAAACACTAC CAAATCAGAA TCAGTCACAA 
ACCCAACCTT TACTGAAGAC TCCTACTGCT GTTATCCAGC CGATTGTGCC ACAGACAACA 
TTTGGTGTTC AGGCACAGCC CCAACCCCAG TCATTATTGC AAGCCCAGAT CTCAGCTGCT 
TCTATTACAC CCCTATTGCA GACGCAGCCA CAGCCCTTAC TACAGCAGCC ACAGCAAAAA 
GCTGGCTTAT TGCAGCCTCC TGTCCGAATA GTATCACAGC CACAACCCGC ACGGAGATTA 
GATCCGCCAT CTAGATTTTC AGGAAGAAAT GACAGAGGGG ATCAAGTACC TAATAGAAAA 
GATGACCGAA GTCGTGAAAG GGACAGAGAA AGACGCAGAT CCAGAGAAAG ATCACCTCAG 
AGGAAACGTT CCCGGGAGAG GTCGCCCCGG AGAGAAAGAG AGCGATCCCC TCGGAGAGTC 
CGCCGTGTCG TTCCACGGTA CACAGTGCAG TTTTCAAAGT TTTCTTTAGA TTGTCCCAGT 
TGTGACATGA TGGAATTAAG GCGCCGTTAT CAGAACTTAT ATATTCCTAG TGACTTTTTT 
GATGCTCAGT TTACATGGGT GGATGCTTTC CCTTTGTCAA GACCATTTCA ACTGGGAAAT 
TACTGCAATT TTTATGTGAT GCACCGAGAA GTGGAGTCCT TAGAAAAAAA TGTGGCTGTT 
CTTGACCCAC CTGATGCTGA CCACCTGTAC AGTGCAAAGG TAATGCTGAT GGCTAGCCCT 
AGTATGGAAG ATTTGTATCA TAAGTCATGT GCTCTTGCTG AAGACCCACA AGACCTTCGT 
GATGGTTTTC AGCATCCTGC TAGACTTGTT AAGTTTCTAG TGGGAATGAA AGGCAAGGAT 
GAAGCCATGG CCATTGGAGG CCACTGGTCT CCTTCGCTGG ATGGACCAAA CCCAGAAAAA 
GACCCCTCTG TGTTGATTAA GACTGCCATT CGTTGTTGTA AGGCCCTGAC AGGCATTGAT 
CTAAGTGTGT GCACACAATG GTACCGTTTT GCAGAGATTC GCTACCATCG CCCTGAGGAG 
ACCCACAAGG GGCGTACAGT TCCAGCTCAT GTGGAGACAG TGGTTTTATT TTTCCCGGAT 
GTTTGGCATT GCCTTCCCAC CCGCTCAGAG TGGGAAACCC TCTCCCGAGG ATACAAGCAG 
CAGCTGGTCG AGAAGCTTCA GGGTGAACGC AAGAAGGCTG ATGGAGAACA GGATGAAGAA 
GAGAAGGATG ATGGTGAAGT TAAAGAAATT GCTACTCCTA CCCATTGGTC TAAACTTGAT 
CCAAAGGCAA TGAAGGTAAA CGATCTCCGA AAAGAATTAG AAAGTCGAGC TCTTAGTTCC 
AAAGGACTAA AATCGCAGTT AATAGCTCGC CTGACAAAGC AGCTTAAAAT AGAAGAACAG 
AAAGAAGAGC AGAAGGAATT AGAGAAGTCT GAAAAGGAAG AGGAAGATGA GGATGATAAG 
AAGTCTGAGG ATGATAAAGA GGAAGAAGAA AGAAAACGTC AAGAAGAAGT GGAACGCCAA 
CGCCAAGAAA GAAGATACAT TTTGCCTGAT GAGCCTGCCA TAATTGTCCA TCCAAACTGG 
GCTGCAAAAA GTGGGAAGTT TGATTGCAGC ATCATGTCTT TGAGTGTCCT TTTAGACTAC 
AGATTGGAAG ATAATAAAGA ACATTCGTTT GAGGTTTCAT TGTTTGCAGA ACTTTTCAAT 
GAAATGCTTC AAAGAGATTT TGGGGTCAGA ATATACAAAT CATTACTCTC TCTTCCTGAG 
AAAGAGGACA AAAAAGATAA GGAAAAGAAA AGCAAAAAAG AAGAGAGAAA AGATAAAAAA 
GAAGAGAGAG AAGATGATAT TGATGAACCA AAACCAAAAC GGAGAAAATC GGGCGATGAT 
AAAGACAAAA AAGAAGACAG AGATGAGAGG AAGAAAGAAG AGAAAAGAAA AGATGATTCT 
AAGGATGATG ATGAAACTGA AGAAGATAAC AATCAGGATG AGTATGACCC CATGGAGGCA 
GAGGAGGCGG AGGAGGAGGA GGACGCGGAC CTGTCTCCCC TTTGCTTTAC AGACAGGGAG 
GAGGAGGAAG TGAACAAACG AGACGACAAA AGGGACGTCA GCCGATACTG CAAGGACAGA 
CCCTCAAAGG ACAAGGAAAA AGAGAAGCCT CAAATGGTCA CAGTTAACAG GGATCTGCTA 
ATGGCCTTTG TTTATTTTGA TCAAAGTCAT TGTGGTTACC TTCTTGAAAA GGATTTGGAA 
GAAATACTTT ATACTCTTGG ACTGCATCTT TCTCGGGCTC AGGTAAAGAA ACTGCTCAAT 
AAAGTAGTGC TCCGAGAATC GTGCTTCTAT CGGAAATTAA CAGACACCTC AAAAGACGGC 
GAGAGCCATG ACGAGCCAGA GTCGCTGCAG GGGGACACGA CGCTGGGAAA CAGGTTACTA 
CTTCCCACAC CAACAGTGAA GCAGGAATCG AAGGATGGAG AAGAAAACGT AGGGCTTATT 
GTGTACAACG GTGCAATGGT GGATGTTGGG AGTCTCTTAC AAAAGCTGGA AAAGAGTGAA 
AAAGTAAGAG CTGAGGTAGA ACAGAAGCTC CAGTTACTAG AGGAGAAAAC AGATGAAGAC 
GGAAAAACTA TATTAAACTT GGAGAATTCT AACAAAAGCC TCTCTGGTGA ACTTAGAGAG 
GTCAAAAAAG ACCTTGGTCA ATTACAAGAA AACTTGAAGG TTTCAGAAAA CATGAATTTG 
CAATTTGAAA ACCAATTGAA TAAAACACTC AGAAACTTAT CCACAGTCAT GGATGATATC 
CACACTGTCC TAAAAAAGGA TAACGTAAAG AATGAAGACA GAGATCAGAA ATCCAAGGAG 
AATGGTTCAA GTGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008771119.1
CDS	146..3043
Translation	MPFKWNAQRIQTLPNQNQSQTQPLLKTPTAVIQPIVPQTTFGVQAQPQPQSLLQAQISAASITPLLQTQPQPLLQQPQQKAGLLQPPVRIVSQPQPARRLDPPSRFSGRNDRGDQVPNRKDDRSRERDRERRRSRERSPQRKRSRERSPRRERERSPRRVRRVVPRYTVQFSKFSLDCPSCDMMELRRRYQNLYIPSDFFDAQFTWVDAFPLSRPFQLGNYCNFYVMHREVESLEKNVAVLDPPDADHLYSAKVMLMASPSMEDLYHKSCALAEDPQDLRDGFQHPARLVKFLVGMKGKDEAMAIGGHWSPSLDGPNPEKDPSVLIKTAIRCCKALTGIDLSVCTQWYRFAEIRYHRPEETHKGRTVPAHVETVVLFFPDVWHCLPTRSEWETLSRGYKQQLVEKLQGERKKADGEQDEEEKDDGEVKEIATPTHWSKLDPKAMKVNDLRKELESRALSSKGLKSQLIARLTKQLKIEEQKEEQKELEKSEKEEEDEDDKKSEDDKEEEERKRQEEVERQRQERRYILPDEPAIIVHPNWAAKSGKFDCSIMSLSVLLDYRLEDNKEHSFEVSLFAELFNEMLQRDFGVRIYKSLLSLPEKEDKKDKEKKSKKEERKDKKEEREDDIDEPKPKRRKSGDDKDKKEDRDERKKEEKRKDDSKDDDETEEDNNQDEYDPMEAEEAEEEEDADLSPLCFTDREEEEVNKRDDKRDVSRYCKDRPSKDKEKEKPQMVTVNRDLLMAFVYFDQSHCGYLLEKDLEEILYTLGLHLSRAQVKKLLNKVVLRESCFYRKLTDTSKDGESHDEPESLQGDTTLGNRLLLPTPTVKQESKDGEENVGLIVYNGAMVDVGSLLQKLEKSEKVRAEVEQKLQLLEEKTDEDGKTILNLENSNKSLSGELREVKKDLGQLQENLKVSENMNLQFENQLNKTLRNLSTVMDDIHTVLKKDNVKNEDRDQKSKENGSSV

Target ORF information:

RefSeq Version	XM_008772897.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cell division cycle and apoptosis regulator 1 (Ccar1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008772897.2

ORF Insert Sequence:

ATGCCTTTTA AATGGAATGC ACAAAGAATT CAAACACTAC CAAATCAGAA TCAGTCACAA 
ACCCAACCTT TACTGAAGAC TCCTACTGCT GTTATCCAGC CGATTGTGCC ACAGACAACA 
TTTGGTGTTC AGGCACAGCC CCAACCCCAG TCATTATTGC AAGCCCAGAT CTCAGCTGCT 
TCTATTACAC CCCTATTGCA GACGCAGCCA CAGCCCTTAC TACAGCAGCC ACAGCAAAAA 
GCTGGCTTAT TGCAGCCTCC TGTCCGAATA GTATCACAGC CACAACCCGC ACGGAGATTA 
GATCCGCCAT CTAGATTTTC AGGAAGAAAT GACAGAGGGG ATCAAGTACC TAATAGAAAA 
GATGACCGAA GTCGTGAAAG GGACAGAGAA AGACGCAGAT CCAGAGAAAG ATCACCTCAG 
AGGAAACGTT CCCGGGAGAG GTCGCCCCGG AGAGAAAGAG AGCGATCCCC TCGGAGAGTC 
CGCCGTGTCG TTCCACGGTA CACAGTGCAG TTTTCAAAGT TTTCTTTAGA TTGTCCCAGT 
TGTGACATGA TGGAATTAAG GCGCCGTTAT CAGAACTTAT ATATTCCTAG TGACTTTTTT 
GATGCTCAGT TTACATGGGT GGATGCTTTC CCTTTGTCAA GACCATTTCA ACTGGGAAAT 
TACTGCAATT TTTATGTGAT GCACCGAGAA GTGGAGTCCT TAGAAAAAAA TGTGGCTGTT 
CTTGACCCAC CTGATGCTGA CCACCTGTAC AGTGCAAAGG TAATGCTGAT GGCTAGCCCT 
AGTATGGAAG ATTTGTATCA TAAGTCATGT GCTCTTGCTG AAGACCCACA AGACCTTCGT 
GATGGTTTTC AGCATCCTGC TAGACTTGTT AAGTTTCTAG TGGGAATGAA AGGCAAGGAT 
GAAGCCATGG CCATTGGAGG CCACTGGTCT CCTTCGCTGG ATGGACCAAA CCCAGAAAAA 
GACCCCTCTG TGTTGATTAA GACTGCCATT CGTTGTTGTA AGGCCCTGAC AGGCATTGAT 
CTAAGTGTGT GCACACAATG GTACCGTTTT GCAGAGATTC GCTACCATCG CCCTGAGGAG 
ACCCACAAGG GGCGTACAGT TCCAGCTCAT GTGGAGACAG TGGTTTTATT TTTCCCGGAT 
GTTTGGCATT GCCTTCCCAC CCGCTCAGAG TGGGAAACCC TCTCCCGAGG ATACAAGCAG 
CAGCTGGTCG AGAAGCTTCA GGGTGAACGC AAGAAGGCTG ATGGAGAACA GGATGAAGAA 
GAGAAGGATG ATGGTGAAGT TAAAGAAATT GCTACTCCTA CCCATTGGTC TAAACTTGAT 
CCAAAGGCAA TGAAGGTAAA CGATCTCCGA AAAGAATTAG AAAGTCGAGC TCTTAGTTCC 
AAAGGACTAA AATCGCAGTT AATAGCTCGC CTGACAAAGC AGCTTAAAAT AGAAGAACAG 
AAAGAAGAGC AGAAGGAATT AGAGAAGTCT GAAAAGGAAG AGGAAGATGA GGATGATAAG 
AAGTCTGAGG ATGATAAAGA GGAAGAAGAA AGAAAACGTC AAGAAGAAGT GGAACGCCAA 
CGCCAAGAAA GAAGATACAT TTTGCCTGAT GAGCCTGCCA TAATTGTCCA TCCAAACTGG 
GCTGCAAAAA GTGGGAAGTT TGATTGCAGC ATCATGTCTT TGAGTGTCCT TTTAGACTAC 
AGATTGGAAG ATAATAAAGA ACATTCGTTT GAGGTTTCAT TGTTTGCAGA ACTTTTCAAT 
GAAATGCTTC AAAGAGATTT TGGGGTCAGA ATATACAAAT CATTACTCTC TCTTCCTGAG 
AAAGAGGACA AAAAAGATAA GGAAAAGAAA AGCAAAAAAG AAGAGAGAAA AGATAAAAAA 
GAAGAGAGAG AAGATGATAT TGATGAACCA AAACCAAAAC GGAGAAAATC GGGCGATGAT 
AAAGACAAAA AAGAAGACAG AGATGAGAGG AAGAAAGAAG AGAAAAGAAA AGATGATTCT 
AAGGATGATG ATGAAACTGA AGAAGATAAC AATCAGGATG AGTATGACCC CATGGAGGCA 
GAGGAGGCGG AGGAGGAGGA GGACGCGGAC CTGTCTCCCC TTTGCTTTAC AGACAGGGAG 
GAGGAGGAAG TGAACAAACG AGACGACAAA AGGGACGTCA GCCGATACTG CAAGGACAGA 
CCCTCAAAGG ACAAGGAAAA AGAGAAGCCT CAAATGGTCA CAGTTAACAG GGATCTGCTA 
ATGGCCTTTG TTTATTTTGA TCAAAGTCAT TGTGGTTACC TTCTTGAAAA GGATTTGGAA 
GAAATACTTT ATACTCTTGG ACTGCATCTT TCTCGGGCTC AGGTAAAGAA ACTGCTCAAT 
AAAGTAGTGC TCCGAGAATC GTGCTTCTAT CGGAAATTAA CAGACACCTC AAAAGACGGC 
GAGAGCCATG ACGAGCCAGA GTCGCTGCAG GGGGACACGA CGCTGGGAAA CAGGTTACTA 
CTTCCCACAC CAACAGTGAA GCAGGAATCG AAGGATGGAG AAGAAAACGT AGGGCTTATT 
GTGTACAACG GTGCAATGGT GGATGTTGGG AGTCTCTTAC AAAAGCTGGA AAAGAGTGAA 
AAAGTAAGAG CTGAGGTAGA ACAGAAGCTC CAGTTACTAG AGGAGAAAAC AGATGAAGAC 
GGAAAAACTA TATTAAACTT GGAGAATTCT AACAAAAGCC TCTCTGGTGA ACTTAGAGAG 
GTCAAAAAAG ACCTTGGTCA ATTACAAGAA AACTTGAAGG TTTCAGAAAA CATGAATTTG 
CAATTTGAAA ACCAATTGAA TAAAACACTC AGAAACTTAT CCACAGTCAT GGATGATATC 
CACACTGTCC TAAAAAAGGA TAACGTAAAG AATGAAGACA GAGATCAGAA ATCCAAGGAG 
AATGGTTCAA GTGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ccar1 ( NM_001108535.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001102005.1 Rattus norvegicus cell division cycle and apoptosis regulator 1 (Ccar1), mRNA.

Price & Availability↑

CloneID	ORa07722
Clone ID Related Accession (Same CDS sequence)	NM_001108535.1
Accession Version	NM_001108535.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3447bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1506787200000
Organism	Rattus norvegicus(Norway rat)
Product	cell division cycle and apoptosis regulator protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH474016.2. On or before Oct 4, 2007 this sequence version replaced XM_342143.3, XM_001058883.1, XM_001058761.1. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCTCAGT TTGGAGGACA GAAGAATCCT CCATGGGCTA CTCAGTTTAC AGCCACGGCG 
GTGTCACAAC CAGCTGCACT AGGTGTTCAG CAGCCATCAC TTCTGGGAGC ATCCCCTACC 
ATCTATACGC AGCAGACTGC ATTGGCGGCC GCAGGCCTTA CCACGCAGAC GCCAGCAAAC 
TATCAGTTAA CACAAACTGC GGCACTGCAG CAGCAAGCTG CAGCTGTCTT ACAGCAGCAA 
TATTCACAAC CTCAGCAGGC CTTGTATAGT GTGCAACAGC AGTTACAGCA ACCCCAGCAG 
ACCATTTTAA CACAGCCAGC TGTTGCATTG CCCACAAGCC TTAGCCTGTC TACTCCTCAG 
CCTGCAGCAC AGATTACTGT GTCCTATCCA ACACCAAGGT CCAGTCAACA GCAGACTCAA 
CCTCAGAAGC AGCGTGTTTT CACAGGAGTG GTTACAAAAC TACATGATAC GTTTGGATTT 
GTGGATGAAG ATGTATTCTT TCAGCTTGGT GCTGTTAAAG GGAAAACCCC CCAAGTGGGT 
GATAGAGTCT TGGTTGAAGC AACCTATAAT CCTAATATGC CTTTTAAATG GAATGCACAA 
AGAATTCAAA CACTACCAAA TCAGAATCAG TCACAAACCC AACCTTTACT GAAGACTCCT 
ACTGCTGTTA TCCAGCCGAT TGTGCCACAG ACAACATTTG GTGTTCAGGC ACAGCCCCAA 
CCCCAGTCAT TATTGCAAGC CCAGATCTCA GCTGCTTCTA TTACACCCCT ATTGCAGACG 
CAGCCACAGC CCTTACTACA GCAGCCACAG CAAAAAGCTG GCTTATTGCA GCCTCCTGTC 
CGAATAGTAT CACAGCCACA ACCCGCACGG AGATTAGATC CGCCATCTAG ATTTTCAGGA 
AGAAATGACA GAGGGGATCA AGTACCTAAT AGAAAAGATG ACCGAAGTCG TGAAAGGGAC 
AGAGAAAGAC GCAGATCCAG AGAAAGATCA CCTCAGAGGA AACGTTCCCG GGAGAGGTCG 
CCCCGGAGAG AAAGAGAGCG ATCCCCTCGG AGAGTCCGCC GTGTCGTTCC ACGGTACACA 
GTGCAGTTTT CAAAGTTTTC TTTAGATTGT CCCAGTTGTG ACATGATGGA ATTAAGGCGC 
CGTTATCAGA ACTTATATAT TCCTAGTGAC TTTTTTGATG CTCAGTTTAC ATGGGTGGAT 
GCTTTCCCTT TGTCAAGACC ATTTCAACTG GGAAATTACT GCAATTTTTA TGTGATGCAC 
CGAGAAGTGG AGTCCTTAGA AAAAAATGTG GCTGTTCTTG ACCCACCTGA TGCTGACCAC 
CTGTACAGTG CAAAGGTAAT GCTGATGGCT AGCCCTAGTA TGGAAGATTT GTATCATAAG 
TCATGTGCTC TTGCTGAAGA CCCACAAGAC CTTCGTGATG GTTTTCAGCA TCCTGCTAGA 
CTTGTTAAGT TTCTAGTGGG AATGAAAGGC AAGGATGAAG CCATGGCCAT TGGAGGCCAC 
TGGTCTCCTT CGCTGGATGG ACCAAACCCA GAAAAAGACC CCTCTGTGTT GATTAAGACT 
GCCATTCGTT GTTGTAAGGC CCTGACAGGC ATTGATCTAA GTGTGTGCAC ACAATGGTAC 
CGTTTTGCAG AGATTCGCTA CCATCGCCCT GAGGAGACCC ACAAGGGGCG TACAGTTCCA 
GCTCATGTGG AGACAGTGGT TTTATTTTTC CCGGATGTTT GGCATTGCCT TCCCACCCGC 
TCAGAGTGGG AAACCCTCTC CCGAGGATAC AAGCAGCAGC TGGTCGAGAA GCTTCAGGGT 
GAACGCAAGA AGGCTGATGG AGAACAGGAT GAAGAAGAGA AGGATGATGG TGAAGTTAAA 
GAAATTGCTA CTCCTACCCA TTGGTCTAAA CTTGATCCAA AGGCAATGAA GGTAAACGAT 
CTCCGAAAAG AATTAGAAAG TCGAGCTCTT AGTTCCAAAG GACTAAAATC GCAGTTAATA 
GCTCGCCTGA CAAAGCAGCT TAAAATAGAA GAACAGAAAG AAGAGCAGAA GGAATTAGAG 
AAGTCTGAAA AGGAAGAGGA AGATGAGGAT GATAAGAAGT CTGAGGATGA TAAAGAGGAA 
GAAGAAAGAA AACGTCAAGA AGAAGTGGAA CGCCAACGCC AAGAAAGAAG ATACATTTTG 
CCTGATGAGC CTGCCATAAT TGTCCATCCA AACTGGGCTG CAAAAAGTGG GAAGTTTGAT 
TGCAGCATCA TGTCTTTGAG TGTCCTTTTA GACTACAGAT TGGAAGATAA TAAAGAACAT 
TCGTTTGAGG TTTCATTGTT TGCAGAACTT TTCAATGAAA TGCTTCAAAG AGATTTTGGG 
GTCAGAATAT ACAAATCATT ACTCTCTCTT CCTGAGAAAG AGGACAAAAA AGATAAGGAA 
AAGAAAAGCA AAAAAGAAGA GAGAAAAGAT AAAAAAGAAG AGAGAGAAGA TGATATTGAT 
GAACCAAAAC CAAAACGGAG AAAATCGGGC GATGATAAAG ACAAAAAAGA AGACAGAGAT 
GAGAGGAAGA AAGAAGAGAA AAGAAAAGAT GATTCTAAGG ATGATGATGA AACTGAAGAA 
GATAACAATC AGGATGAGTA TGACCCCATG GAGGCAGAGG AGGCGGAGGA GGAGGAGGAC 
GACAGGGAGG AGGAGGAAGT GAACAAACGA GACGACAAAA GGGACGTCAG CCGATACTGC 
AAGGACAGAC CCTCAAAGGA CAAGGAAAAA GAGAAGCCTC AAATGGTCAC AGTTAACAGG 
GATCTGCTAA TGGCCTTTGT TTATTTTGAT CAAAGTCATT GTGGTTACCT TCTTGAAAAG 
GATTTGGAAG AAATACTTTA TACTCTTGGA CTGCATCTTT CTCGGGCTCA GGTAAAGAAA 
CTGCTCAATA AAGTAGTGCT CCGAGAATCG TGCTTCTATC GGAAATTAAC AGACACCTCA 
AAAGACGGCG AGAGCCATGA CGAGCCAGAG TCGCTGCAGG GGGACACGAC GCTGGGAAAC 
AGGTTACTAC TTCCCACACC AACAGTGAAG CAGGAATCGA AGGATGGAGA AGAAAACGTA 
GGGCTTATTG TGTACAACGG TGCAATGGTG GATGTTGGGA GTCTCTTACA AAAGCTGGAA 
AAGAGTGAAA AAGTAAGAGC TGAGGTAGAA CAGAAGCTCC AGTTACTAGA GGAGAAAACA 
GATGAAGACG GAAAAACTAT ATTAAACTTG GAGAATTCTA ACAAAAGCCT CTCTGGTGAA 
CTTAGAGAGG TCAAAAAAGA CCTTGGTCAA TTACAAGAAA ACTTGAAGGT TTCAGAAAAC 
ATGAATTTGC AATTTGAAAA CCAATTGAAT AAAACACTCA GAAACTTATC CACAGTCATG 
GATGATATCC ACACTGTCCT AAAAAAGGAT AACGTAAAGA ATGAAGACAG AGATCAGAAA 
TCCAAGGAGA ATGGTTCAAG TGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001102005.1
CDS	189..3635
Translation	MAQFGGQKNPPWATQFTATAVSQPAALGVQQPSLLGASPTIYTQQTALAAAGLTTQTPANYQLTQTAALQQQAAAVLQQQYSQPQQALYSVQQQLQQPQQTILTQPAVALPTSLSLSTPQPAAQITVSYPTPRSSQQQTQPQKQRVFTGVVTKLHDTFGFVDEDVFFQLGAVKGKTPQVGDRVLVEATYNPNMPFKWNAQRIQTLPNQNQSQTQPLLKTPTAVIQPIVPQTTFGVQAQPQPQSLLQAQISAASITPLLQTQPQPLLQQPQQKAGLLQPPVRIVSQPQPARRLDPPSRFSGRNDRGDQVPNRKDDRSRERDRERRRSRERSPQRKRSRERSPRRERERSPRRVRRVVPRYTVQFSKFSLDCPSCDMMELRRRYQNLYIPSDFFDAQFTWVDAFPLSRPFQLGNYCNFYVMHREVESLEKNVAVLDPPDADHLYSAKVMLMASPSMEDLYHKSCALAEDPQDLRDGFQHPARLVKFLVGMKGKDEAMAIGGHWSPSLDGPNPEKDPSVLIKTAIRCCKALTGIDLSVCTQWYRFAEIRYHRPEETHKGRTVPAHVETVVLFFPDVWHCLPTRSEWETLSRGYKQQLVEKLQGERKKADGEQDEEEKDDGEVKEIATPTHWSKLDPKAMKVNDLRKELESRALSSKGLKSQLIARLTKQLKIEEQKEEQKELEKSEKEEEDEDDKKSEDDKEEEERKRQEEVERQRQERRYILPDEPAIIVHPNWAAKSGKFDCSIMSLSVLLDYRLEDNKEHSFEVSLFAELFNEMLQRDFGVRIYKSLLSLPEKEDKKDKEKKSKKEERKDKKEEREDDIDEPKPKRRKSGDDKDKKEDRDERKKEEKRKDDSKDDDETEEDNNQDEYDPMEAEEAEEEEDDREEEEVNKRDDKRDVSRYCKDRPSKDKEKEKPQMVTVNRDLLMAFVYFDQSHCGYLLEKDLEEILYTLGLHLSRAQVKKLLNKVVLRESCFYRKLTDTSKDGESHDEPESLQGDTTLGNRLLLPTPTVKQESKDGEENVGLIVYNGAMVDVGSLLQKLEKSEKVRAEVEQKLQLLEEKTDEDGKTILNLENSNKSLSGELREVKKDLGQLQENLKVSENMNLQFENQLNKTLRNLSTVMDDIHTVLKKDNVKNEDRDQKSKENGSSV

Target ORF information:

RefSeq Version	NM_001108535.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cell division cycle and apoptosis regulator 1 (Ccar1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001108535.1

ORF Insert Sequence:

ATGGCTCAGT TTGGAGGACA GAAGAATCCT CCATGGGCTA CTCAGTTTAC AGCCACGGCG 
GTGTCACAAC CAGCTGCACT AGGTGTTCAG CAGCCATCAC TTCTGGGAGC ATCCCCTACC 
ATCTATACGC AGCAGACTGC ATTGGCGGCC GCAGGCCTTA CCACGCAGAC GCCAGCAAAC 
TATCAGTTAA CACAAACTGC GGCACTGCAG CAGCAAGCTG CAGCTGTCTT ACAGCAGCAA 
TATTCACAAC CTCAGCAGGC CTTGTATAGT GTGCAACAGC AGTTACAGCA ACCCCAGCAG 
ACCATTTTAA CACAGCCAGC TGTTGCATTG CCCACAAGCC TTAGCCTGTC TACTCCTCAG 
CCTGCAGCAC AGATTACTGT GTCCTATCCA ACACCAAGGT CCAGTCAACA GCAGACTCAA 
CCTCAGAAGC AGCGTGTTTT CACAGGAGTG GTTACAAAAC TACATGATAC GTTTGGATTT 
GTGGATGAAG ATGTATTCTT TCAGCTTGGT GCTGTTAAAG GGAAAACCCC CCAAGTGGGT 
GATAGAGTCT TGGTTGAAGC AACCTATAAT CCTAATATGC CTTTTAAATG GAATGCACAA 
AGAATTCAAA CACTACCAAA TCAGAATCAG TCACAAACCC AACCTTTACT GAAGACTCCT 
ACTGCTGTTA TCCAGCCGAT TGTGCCACAG ACAACATTTG GTGTTCAGGC ACAGCCCCAA 
CCCCAGTCAT TATTGCAAGC CCAGATCTCA GCTGCTTCTA TTACACCCCT ATTGCAGACG 
CAGCCACAGC CCTTACTACA GCAGCCACAG CAAAAAGCTG GCTTATTGCA GCCTCCTGTC 
CGAATAGTAT CACAGCCACA ACCCGCACGG AGATTAGATC CGCCATCTAG ATTTTCAGGA 
AGAAATGACA GAGGGGATCA AGTACCTAAT AGAAAAGATG ACCGAAGTCG TGAAAGGGAC 
AGAGAAAGAC GCAGATCCAG AGAAAGATCA CCTCAGAGGA AACGTTCCCG GGAGAGGTCG 
CCCCGGAGAG AAAGAGAGCG ATCCCCTCGG AGAGTCCGCC GTGTCGTTCC ACGGTACACA 
GTGCAGTTTT CAAAGTTTTC TTTAGATTGT CCCAGTTGTG ACATGATGGA ATTAAGGCGC 
CGTTATCAGA ACTTATATAT TCCTAGTGAC TTTTTTGATG CTCAGTTTAC ATGGGTGGAT 
GCTTTCCCTT TGTCAAGACC ATTTCAACTG GGAAATTACT GCAATTTTTA TGTGATGCAC 
CGAGAAGTGG AGTCCTTAGA AAAAAATGTG GCTGTTCTTG ACCCACCTGA TGCTGACCAC 
CTGTACAGTG CAAAGGTAAT GCTGATGGCT AGCCCTAGTA TGGAAGATTT GTATCATAAG 
TCATGTGCTC TTGCTGAAGA CCCACAAGAC CTTCGTGATG GTTTTCAGCA TCCTGCTAGA 
CTTGTTAAGT TTCTAGTGGG AATGAAAGGC AAGGATGAAG CCATGGCCAT TGGAGGCCAC 
TGGTCTCCTT CGCTGGATGG ACCAAACCCA GAAAAAGACC CCTCTGTGTT GATTAAGACT 
GCCATTCGTT GTTGTAAGGC CCTGACAGGC ATTGATCTAA GTGTGTGCAC ACAATGGTAC 
CGTTTTGCAG AGATTCGCTA CCATCGCCCT GAGGAGACCC ACAAGGGGCG TACAGTTCCA 
GCTCATGTGG AGACAGTGGT TTTATTTTTC CCGGATGTTT GGCATTGCCT TCCCACCCGC 
TCAGAGTGGG AAACCCTCTC CCGAGGATAC AAGCAGCAGC TGGTCGAGAA GCTTCAGGGT 
GAACGCAAGA AGGCTGATGG AGAACAGGAT GAAGAAGAGA AGGATGATGG TGAAGTTAAA 
GAAATTGCTA CTCCTACCCA TTGGTCTAAA CTTGATCCAA AGGCAATGAA GGTAAACGAT 
CTCCGAAAAG AATTAGAAAG TCGAGCTCTT AGTTCCAAAG GACTAAAATC GCAGTTAATA 
GCTCGCCTGA CAAAGCAGCT TAAAATAGAA GAACAGAAAG AAGAGCAGAA GGAATTAGAG 
AAGTCTGAAA AGGAAGAGGA AGATGAGGAT GATAAGAAGT CTGAGGATGA TAAAGAGGAA 
GAAGAAAGAA AACGTCAAGA AGAAGTGGAA CGCCAACGCC AAGAAAGAAG ATACATTTTG 
CCTGATGAGC CTGCCATAAT TGTCCATCCA AACTGGGCTG CAAAAAGTGG GAAGTTTGAT 
TGCAGCATCA TGTCTTTGAG TGTCCTTTTA GACTACAGAT TGGAAGATAA TAAAGAACAT 
TCGTTTGAGG TTTCATTGTT TGCAGAACTT TTCAATGAAA TGCTTCAAAG AGATTTTGGG 
GTCAGAATAT ACAAATCATT ACTCTCTCTT CCTGAGAAAG AGGACAAAAA AGATAAGGAA 
AAGAAAAGCA AAAAAGAAGA GAGAAAAGAT AAAAAAGAAG AGAGAGAAGA TGATATTGAT 
GAACCAAAAC CAAAACGGAG AAAATCGGGC GATGATAAAG ACAAAAAAGA AGACAGAGAT 
GAGAGGAAGA AAGAAGAGAA AAGAAAAGAT GATTCTAAGG ATGATGATGA AACTGAAGAA 
GATAACAATC AGGATGAGTA TGACCCCATG GAGGCAGAGG AGGCGGAGGA GGAGGAGGAC 
GACAGGGAGG AGGAGGAAGT GAACAAACGA GACGACAAAA GGGACGTCAG CCGATACTGC 
AAGGACAGAC CCTCAAAGGA CAAGGAAAAA GAGAAGCCTC AAATGGTCAC AGTTAACAGG 
GATCTGCTAA TGGCCTTTGT TTATTTTGAT CAAAGTCATT GTGGTTACCT TCTTGAAAAG 
GATTTGGAAG AAATACTTTA TACTCTTGGA CTGCATCTTT CTCGGGCTCA GGTAAAGAAA 
CTGCTCAATA AAGTAGTGCT CCGAGAATCG TGCTTCTATC GGAAATTAAC AGACACCTCA 
AAAGACGGCG AGAGCCATGA CGAGCCAGAG TCGCTGCAGG GGGACACGAC GCTGGGAAAC 
AGGTTACTAC TTCCCACACC AACAGTGAAG CAGGAATCGA AGGATGGAGA AGAAAACGTA 
GGGCTTATTG TGTACAACGG TGCAATGGTG GATGTTGGGA GTCTCTTACA AAAGCTGGAA 
AAGAGTGAAA AAGTAAGAGC TGAGGTAGAA CAGAAGCTCC AGTTACTAGA GGAGAAAACA 
GATGAAGACG GAAAAACTAT ATTAAACTTG GAGAATTCTA ACAAAAGCCT CTCTGGTGAA 
CTTAGAGAGG TCAAAAAAGA CCTTGGTCAA TTACAAGAAA ACTTGAAGGT TTCAGAAAAC 
ATGAATTTGC AATTTGAAAA CCAATTGAAT AAAACACTCA GAAACTTATC CACAGTCATG 
GATGATATCC ACACTGTCCT AAAAAAGGAT AACGTAAAGA ATGAAGACAG AGATCAGAAA 
TCCAAGGAGA ATGGTTCAAG TGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ccar1 ( XM_006256462.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006256524.1 Rattus norvegicus cell division cycle and apoptosis regulator 1 (Ccar1), transcript variant X1, mRNA.

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CloneID	ORa39466
Clone ID Related Accession (Same CDS sequence)	XM_006256462.2 , XM_008772894.1 , XM_006256463.2
Accession Version	XM_006256462.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3474bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	cell division cycle and apoptosis regulator protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005119.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006256462.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGT TTGGAGGACA GAAGAATCCT CCATGGGCTA CTCAGTTTAC AGCCACGGCG 
GTGTCACAAC CAGCTGCACT AGGTGTTCAG CAGCCATCAC TTCTGGGAGC ATCCCCTACC 
ATCTATACGC AGCAGACTGC ATTGGCGGCC GCAGGCCTTA CCACGCAGAC GCCAGCAAAC 
TATCAGTTAA CACAAACTGC GGCACTGCAG CAGCAAGCTG CAGCTGTCTT ACAGCAGCAA 
TATTCACAAC CTCAGCAGGC CTTGTATAGT GTGCAACAGC AGTTACAGCA ACCCCAGCAG 
ACCATTTTAA CACAGCCAGC TGTTGCATTG CCCACAAGCC TTAGCCTGTC TACTCCTCAG 
CCTGCAGCAC AGATTACTGT GTCCTATCCA ACACCAAGGT CCAGTCAACA GCAGACTCAA 
CCTCAGAAGC AGCGTGTTTT CACAGGAGTG GTTACAAAAC TACATGATAC GTTTGGATTT 
GTGGATGAAG ATGTATTCTT TCAGCTTGGT GCTGTTAAAG GGAAAACCCC CCAAGTGGGT 
GATAGAGTCT TGGTTGAAGC AACCTATAAT CCTAATATGC CTTTTAAATG GAATGCACAA 
AGAATTCAAA CACTACCAAA TCAGAATCAG TCACAAACCC AACCTTTACT GAAGACTCCT 
ACTGCTGTTA TCCAGCCGAT TGTGCCACAG ACAACATTTG GTGTTCAGGC ACAGCCCCAA 
CCCCAGTCAT TATTGCAAGC CCAGATCTCA GCTGCTTCTA TTACACCCCT ATTGCAGACG 
CAGCCACAGC CCTTACTACA GCAGCCACAG CAAAAAGCTG GCTTATTGCA GCCTCCTGTC 
CGAATAGTAT CACAGCCACA ACCCGCACGG AGATTAGATC CGCCATCTAG ATTTTCAGGA 
AGAAATGACA GAGGGGATCA AGTACCTAAT AGAAAAGATG ACCGAAGTCG TGAAAGGGAC 
AGAGAAAGAC GCAGATCCAG AGAAAGATCA CCTCAGAGGA AACGTTCCCG GGAGAGGTCG 
CCCCGGAGAG AAAGAGAGCG ATCCCCTCGG AGAGTCCGCC GTGTCGTTCC ACGGTACACA 
GTGCAGTTTT CAAAGTTTTC TTTAGATTGT CCCAGTTGTG ACATGATGGA ATTAAGGCGC 
CGTTATCAGA ACTTATATAT TCCTAGTGAC TTTTTTGATG CTCAGTTTAC ATGGGTGGAT 
GCTTTCCCTT TGTCAAGACC ATTTCAACTG GGAAATTACT GCAATTTTTA TGTGATGCAC 
CGAGAAGTGG AGTCCTTAGA AAAAAATGTG GCTGTTCTTG ACCCACCTGA TGCTGACCAC 
CTGTACAGTG CAAAGGTAAT GCTGATGGCT AGCCCTAGTA TGGAAGATTT GTATCATAAG 
TCATGTGCTC TTGCTGAAGA CCCACAAGAC CTTCGTGATG GTTTTCAGCA TCCTGCTAGA 
CTTGTTAAGT TTCTAGTGGG AATGAAAGGC AAGGATGAAG CCATGGCCAT TGGAGGCCAC 
TGGTCTCCTT CGCTGGATGG ACCAAACCCA GAAAAAGACC CCTCTGTGTT GATTAAGACT 
GCCATTCGTT GTTGTAAGGC CCTGACAGGC ATTGATCTAA GTGTGTGCAC ACAATGGTAC 
CGTTTTGCAG AGATTCGCTA CCATCGCCCT GAGGAGACCC ACAAGGGGCG TACAGTTCCA 
GCTCATGTGG AGACAGTGGT TTTATTTTTC CCGGATGTTT GGCATTGCCT TCCCACCCGC 
TCAGAGTGGG AAACCCTCTC CCGAGGATAC AAGCAGCAGC TGGTCGAGAA GCTTCAGGGT 
GAACGCAAGA AGGCTGATGG AGAACAGGAT GAAGAAGAGA AGGATGATGG TGAAGTTAAA 
GAAATTGCTA CTCCTACCCA TTGGTCTAAA CTTGATCCAA AGGCAATGAA GGTAAACGAT 
CTCCGAAAAG AATTAGAAAG TCGAGCTCTT AGTTCCAAAG GACTAAAATC GCAGTTAATA 
GCTCGCCTGA CAAAGCAGCT TAAAATAGAA GAACAGAAAG AAGAGCAGAA GGAATTAGAG 
AAGTCTGAAA AGGAAGAGGA AGATGAGGAT GATAAGAAGT CTGAGGATGA TAAAGAGGAA 
GAAGAAAGAA AACGTCAAGA AGAAGTGGAA CGCCAACGCC AAGAAAGAAG ATACATTTTG 
CCTGATGAGC CTGCCATAAT TGTCCATCCA AACTGGGCTG CAAAAAGTGG GAAGTTTGAT 
TGCAGCATCA TGTCTTTGAG TGTCCTTTTA GACTACAGAT TGGAAGATAA TAAAGAACAT 
TCGTTTGAGG TTTCATTGTT TGCAGAACTT TTCAATGAAA TGCTTCAAAG AGATTTTGGG 
GTCAGAATAT ACAAATCATT ACTCTCTCTT CCTGAGAAAG AGGACAAAAA AGATAAGGAA 
AAGAAAAGCA AAAAAGAAGA GAGAAAAGAT AAAAAAGAAG AGAGAGAAGA TGATATTGAT 
GAACCAAAAC CAAAACGGAG AAAATCGGGC GATGATAAAG ACAAAAAAGA AGACAGAGAT 
GAGAGGAAGA AAGAAGAGAA AAGAAAAGAT GATTCTAAGG ATGATGATGA AACTGAAGAA 
GATAACAATC AGGATGAGTA TGACCCCATG GAGGCAGAGG AGGCGGAGGA GGAGGAGGAC 
GCGGACCTGT CTCCCCTTTG CTTTACAGAC AGGGAGGAGG AGGAAGTGAA CAAACGAGAC 
GACAAAAGGG ACGTCAGCCG ATACTGCAAG GACAGACCCT CAAAGGACAA GGAAAAAGAG 
AAGCCTCAAA TGGTCACAGT TAACAGGGAT CTGCTAATGG CCTTTGTTTA TTTTGATCAA 
AGTCATTGTG GTTACCTTCT TGAAAAGGAT TTGGAAGAAA TACTTTATAC TCTTGGACTG 
CATCTTTCTC GGGCTCAGGT AAAGAAACTG CTCAATAAAG TAGTGCTCCG AGAATCGTGC 
TTCTATCGGA AATTAACAGA CACCTCAAAA GACGGCGAGA GCCATGACGA GCCAGAGTCG 
CTGCAGGGGG ACACGACGCT GGGAAACAGG TTACTACTTC CCACACCAAC AGTGAAGCAG 
GAATCGAAGG ATGGAGAAGA AAACGTAGGG CTTATTGTGT ACAACGGTGC AATGGTGGAT 
GTTGGGAGTC TCTTACAAAA GCTGGAAAAG AGTGAAAAAG TAAGAGCTGA GGTAGAACAG 
AAGCTCCAGT TACTAGAGGA GAAAACAGAT GAAGACGGAA AAACTATATT AAACTTGGAG 
AATTCTAACA AAAGCCTCTC TGGTGAACTT AGAGAGGTCA AAAAAGACCT TGGTCAATTA 
CAAGAAAACT TGAAGGTTTC AGAAAACATG AATTTGCAAT TTGAAAACCA ATTGAATAAA 
ACACTCAGAA ACTTATCCAC AGTCATGGAT GATATCCACA CTGTCCTAAA AAAGGATAAC 
GTAAAGAATG AAGACAGAGA TCAGAAATCC AAGGAGAATG GTTCAAGTGT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006256524.1
CDS	142..3615
Translation	MAQFGGQKNPPWATQFTATAVSQPAALGVQQPSLLGASPTIYTQQTALAAAGLTTQTPANYQLTQTAALQQQAAAVLQQQYSQPQQALYSVQQQLQQPQQTILTQPAVALPTSLSLSTPQPAAQITVSYPTPRSSQQQTQPQKQRVFTGVVTKLHDTFGFVDEDVFFQLGAVKGKTPQVGDRVLVEATYNPNMPFKWNAQRIQTLPNQNQSQTQPLLKTPTAVIQPIVPQTTFGVQAQPQPQSLLQAQISAASITPLLQTQPQPLLQQPQQKAGLLQPPVRIVSQPQPARRLDPPSRFSGRNDRGDQVPNRKDDRSRERDRERRRSRERSPQRKRSRERSPRRERERSPRRVRRVVPRYTVQFSKFSLDCPSCDMMELRRRYQNLYIPSDFFDAQFTWVDAFPLSRPFQLGNYCNFYVMHREVESLEKNVAVLDPPDADHLYSAKVMLMASPSMEDLYHKSCALAEDPQDLRDGFQHPARLVKFLVGMKGKDEAMAIGGHWSPSLDGPNPEKDPSVLIKTAIRCCKALTGIDLSVCTQWYRFAEIRYHRPEETHKGRTVPAHVETVVLFFPDVWHCLPTRSEWETLSRGYKQQLVEKLQGERKKADGEQDEEEKDDGEVKEIATPTHWSKLDPKAMKVNDLRKELESRALSSKGLKSQLIARLTKQLKIEEQKEEQKELEKSEKEEEDEDDKKSEDDKEEEERKRQEEVERQRQERRYILPDEPAIIVHPNWAAKSGKFDCSIMSLSVLLDYRLEDNKEHSFEVSLFAELFNEMLQRDFGVRIYKSLLSLPEKEDKKDKEKKSKKEERKDKKEEREDDIDEPKPKRRKSGDDKDKKEDRDERKKEEKRKDDSKDDDETEEDNNQDEYDPMEAEEAEEEEDADLSPLCFTDREEEEVNKRDDKRDVSRYCKDRPSKDKEKEKPQMVTVNRDLLMAFVYFDQSHCGYLLEKDLEEILYTLGLHLSRAQVKKLLNKVVLRESCFYRKLTDTSKDGESHDEPESLQGDTTLGNRLLLPTPTVKQESKDGEENVGLIVYNGAMVDVGSLLQKLEKSEKVRAEVEQKLQLLEEKTDEDGKTILNLENSNKSLSGELREVKKDLGQLQENLKVSENMNLQFENQLNKTLRNLSTVMDDIHTVLKKDNVKNEDRDQKSKENGSSV

Target ORF information:

RefSeq Version	XM_006256462.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cell division cycle and apoptosis regulator 1 (Ccar1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006256462.2

ORF Insert Sequence:

ATGGCTCAGT TTGGAGGACA GAAGAATCCT CCATGGGCTA CTCAGTTTAC AGCCACGGCG 
GTGTCACAAC CAGCTGCACT AGGTGTTCAG CAGCCATCAC TTCTGGGAGC ATCCCCTACC 
ATCTATACGC AGCAGACTGC ATTGGCGGCC GCAGGCCTTA CCACGCAGAC GCCAGCAAAC 
TATCAGTTAA CACAAACTGC GGCACTGCAG CAGCAAGCTG CAGCTGTCTT ACAGCAGCAA 
TATTCACAAC CTCAGCAGGC CTTGTATAGT GTGCAACAGC AGTTACAGCA ACCCCAGCAG 
ACCATTTTAA CACAGCCAGC TGTTGCATTG CCCACAAGCC TTAGCCTGTC TACTCCTCAG 
CCTGCAGCAC AGATTACTGT GTCCTATCCA ACACCAAGGT CCAGTCAACA GCAGACTCAA 
CCTCAGAAGC AGCGTGTTTT CACAGGAGTG GTTACAAAAC TACATGATAC GTTTGGATTT 
GTGGATGAAG ATGTATTCTT TCAGCTTGGT GCTGTTAAAG GGAAAACCCC CCAAGTGGGT 
GATAGAGTCT TGGTTGAAGC AACCTATAAT CCTAATATGC CTTTTAAATG GAATGCACAA 
AGAATTCAAA CACTACCAAA TCAGAATCAG TCACAAACCC AACCTTTACT GAAGACTCCT 
ACTGCTGTTA TCCAGCCGAT TGTGCCACAG ACAACATTTG GTGTTCAGGC ACAGCCCCAA 
CCCCAGTCAT TATTGCAAGC CCAGATCTCA GCTGCTTCTA TTACACCCCT ATTGCAGACG 
CAGCCACAGC CCTTACTACA GCAGCCACAG CAAAAAGCTG GCTTATTGCA GCCTCCTGTC 
CGAATAGTAT CACAGCCACA ACCCGCACGG AGATTAGATC CGCCATCTAG ATTTTCAGGA 
AGAAATGACA GAGGGGATCA AGTACCTAAT AGAAAAGATG ACCGAAGTCG TGAAAGGGAC 
AGAGAAAGAC GCAGATCCAG AGAAAGATCA CCTCAGAGGA AACGTTCCCG GGAGAGGTCG 
CCCCGGAGAG AAAGAGAGCG ATCCCCTCGG AGAGTCCGCC GTGTCGTTCC ACGGTACACA 
GTGCAGTTTT CAAAGTTTTC TTTAGATTGT CCCAGTTGTG ACATGATGGA ATTAAGGCGC 
CGTTATCAGA ACTTATATAT TCCTAGTGAC TTTTTTGATG CTCAGTTTAC ATGGGTGGAT 
GCTTTCCCTT TGTCAAGACC ATTTCAACTG GGAAATTACT GCAATTTTTA TGTGATGCAC 
CGAGAAGTGG AGTCCTTAGA AAAAAATGTG GCTGTTCTTG ACCCACCTGA TGCTGACCAC 
CTGTACAGTG CAAAGGTAAT GCTGATGGCT AGCCCTAGTA TGGAAGATTT GTATCATAAG 
TCATGTGCTC TTGCTGAAGA CCCACAAGAC CTTCGTGATG GTTTTCAGCA TCCTGCTAGA 
CTTGTTAAGT TTCTAGTGGG AATGAAAGGC AAGGATGAAG CCATGGCCAT TGGAGGCCAC 
TGGTCTCCTT CGCTGGATGG ACCAAACCCA GAAAAAGACC CCTCTGTGTT GATTAAGACT 
GCCATTCGTT GTTGTAAGGC CCTGACAGGC ATTGATCTAA GTGTGTGCAC ACAATGGTAC 
CGTTTTGCAG AGATTCGCTA CCATCGCCCT GAGGAGACCC ACAAGGGGCG TACAGTTCCA 
GCTCATGTGG AGACAGTGGT TTTATTTTTC CCGGATGTTT GGCATTGCCT TCCCACCCGC 
TCAGAGTGGG AAACCCTCTC CCGAGGATAC AAGCAGCAGC TGGTCGAGAA GCTTCAGGGT 
GAACGCAAGA AGGCTGATGG AGAACAGGAT GAAGAAGAGA AGGATGATGG TGAAGTTAAA 
GAAATTGCTA CTCCTACCCA TTGGTCTAAA CTTGATCCAA AGGCAATGAA GGTAAACGAT 
CTCCGAAAAG AATTAGAAAG TCGAGCTCTT AGTTCCAAAG GACTAAAATC GCAGTTAATA 
GCTCGCCTGA CAAAGCAGCT TAAAATAGAA GAACAGAAAG AAGAGCAGAA GGAATTAGAG 
AAGTCTGAAA AGGAAGAGGA AGATGAGGAT GATAAGAAGT CTGAGGATGA TAAAGAGGAA 
GAAGAAAGAA AACGTCAAGA AGAAGTGGAA CGCCAACGCC AAGAAAGAAG ATACATTTTG 
CCTGATGAGC CTGCCATAAT TGTCCATCCA AACTGGGCTG CAAAAAGTGG GAAGTTTGAT 
TGCAGCATCA TGTCTTTGAG TGTCCTTTTA GACTACAGAT TGGAAGATAA TAAAGAACAT 
TCGTTTGAGG TTTCATTGTT TGCAGAACTT TTCAATGAAA TGCTTCAAAG AGATTTTGGG 
GTCAGAATAT ACAAATCATT ACTCTCTCTT CCTGAGAAAG AGGACAAAAA AGATAAGGAA 
AAGAAAAGCA AAAAAGAAGA GAGAAAAGAT AAAAAAGAAG AGAGAGAAGA TGATATTGAT 
GAACCAAAAC CAAAACGGAG AAAATCGGGC GATGATAAAG ACAAAAAAGA AGACAGAGAT 
GAGAGGAAGA AAGAAGAGAA AAGAAAAGAT GATTCTAAGG ATGATGATGA AACTGAAGAA 
GATAACAATC AGGATGAGTA TGACCCCATG GAGGCAGAGG AGGCGGAGGA GGAGGAGGAC 
GCGGACCTGT CTCCCCTTTG CTTTACAGAC AGGGAGGAGG AGGAAGTGAA CAAACGAGAC 
GACAAAAGGG ACGTCAGCCG ATACTGCAAG GACAGACCCT CAAAGGACAA GGAAAAAGAG 
AAGCCTCAAA TGGTCACAGT TAACAGGGAT CTGCTAATGG CCTTTGTTTA TTTTGATCAA 
AGTCATTGTG GTTACCTTCT TGAAAAGGAT TTGGAAGAAA TACTTTATAC TCTTGGACTG 
CATCTTTCTC GGGCTCAGGT AAAGAAACTG CTCAATAAAG TAGTGCTCCG AGAATCGTGC 
TTCTATCGGA AATTAACAGA CACCTCAAAA GACGGCGAGA GCCATGACGA GCCAGAGTCG 
CTGCAGGGGG ACACGACGCT GGGAAACAGG TTACTACTTC CCACACCAAC AGTGAAGCAG 
GAATCGAAGG ATGGAGAAGA AAACGTAGGG CTTATTGTGT ACAACGGTGC AATGGTGGAT 
GTTGGGAGTC TCTTACAAAA GCTGGAAAAG AGTGAAAAAG TAAGAGCTGA GGTAGAACAG 
AAGCTCCAGT TACTAGAGGA GAAAACAGAT GAAGACGGAA AAACTATATT AAACTTGGAG 
AATTCTAACA AAAGCCTCTC TGGTGAACTT AGAGAGGTCA AAAAAGACCT TGGTCAATTA 
CAAGAAAACT TGAAGGTTTC AGAAAACATG AATTTGCAAT TTGAAAACCA ATTGAATAAA 
ACACTCAGAA ACTTATCCAC AGTCATGGAT GATATCCACA CTGTCCTAAA AAAGGATAAC 
GTAAAGAATG AAGACAGAGA TCAGAAATCC AAGGAGAATG GTTCAAGTGT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ccar1 ( XM_008772894.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008771116.1 Rattus norvegicus cell division cycle and apoptosis regulator 1 (Ccar1), transcript variant X3, mRNA.

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CloneID	ORa39466
Clone ID Related Accession (Same CDS sequence)	XM_006256462.2 , XM_008772894.1 , XM_006256463.2
Accession Version	XM_008772894.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3474bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	cell division cycle and apoptosis regulator protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005119.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGT TTGGAGGACA GAAGAATCCT CCATGGGCTA CTCAGTTTAC AGCCACGGCG 
GTGTCACAAC CAGCTGCACT AGGTGTTCAG CAGCCATCAC TTCTGGGAGC ATCCCCTACC 
ATCTATACGC AGCAGACTGC ATTGGCGGCC GCAGGCCTTA CCACGCAGAC GCCAGCAAAC 
TATCAGTTAA CACAAACTGC GGCACTGCAG CAGCAAGCTG CAGCTGTCTT ACAGCAGCAA 
TATTCACAAC CTCAGCAGGC CTTGTATAGT GTGCAACAGC AGTTACAGCA ACCCCAGCAG 
ACCATTTTAA CACAGCCAGC TGTTGCATTG CCCACAAGCC TTAGCCTGTC TACTCCTCAG 
CCTGCAGCAC AGATTACTGT GTCCTATCCA ACACCAAGGT CCAGTCAACA GCAGACTCAA 
CCTCAGAAGC AGCGTGTTTT CACAGGAGTG GTTACAAAAC TACATGATAC GTTTGGATTT 
GTGGATGAAG ATGTATTCTT TCAGCTTGGT GCTGTTAAAG GGAAAACCCC CCAAGTGGGT 
GATAGAGTCT TGGTTGAAGC AACCTATAAT CCTAATATGC CTTTTAAATG GAATGCACAA 
AGAATTCAAA CACTACCAAA TCAGAATCAG TCACAAACCC AACCTTTACT GAAGACTCCT 
ACTGCTGTTA TCCAGCCGAT TGTGCCACAG ACAACATTTG GTGTTCAGGC ACAGCCCCAA 
CCCCAGTCAT TATTGCAAGC CCAGATCTCA GCTGCTTCTA TTACACCCCT ATTGCAGACG 
CAGCCACAGC CCTTACTACA GCAGCCACAG CAAAAAGCTG GCTTATTGCA GCCTCCTGTC 
CGAATAGTAT CACAGCCACA ACCCGCACGG AGATTAGATC CGCCATCTAG ATTTTCAGGA 
AGAAATGACA GAGGGGATCA AGTACCTAAT AGAAAAGATG ACCGAAGTCG TGAAAGGGAC 
AGAGAAAGAC GCAGATCCAG AGAAAGATCA CCTCAGAGGA AACGTTCCCG GGAGAGGTCG 
CCCCGGAGAG AAAGAGAGCG ATCCCCTCGG AGAGTCCGCC GTGTCGTTCC ACGGTACACA 
GTGCAGTTTT CAAAGTTTTC TTTAGATTGT CCCAGTTGTG ACATGATGGA ATTAAGGCGC 
CGTTATCAGA ACTTATATAT TCCTAGTGAC TTTTTTGATG CTCAGTTTAC ATGGGTGGAT 
GCTTTCCCTT TGTCAAGACC ATTTCAACTG GGAAATTACT GCAATTTTTA TGTGATGCAC 
CGAGAAGTGG AGTCCTTAGA AAAAAATGTG GCTGTTCTTG ACCCACCTGA TGCTGACCAC 
CTGTACAGTG CAAAGGTAAT GCTGATGGCT AGCCCTAGTA TGGAAGATTT GTATCATAAG 
TCATGTGCTC TTGCTGAAGA CCCACAAGAC CTTCGTGATG GTTTTCAGCA TCCTGCTAGA 
CTTGTTAAGT TTCTAGTGGG AATGAAAGGC AAGGATGAAG CCATGGCCAT TGGAGGCCAC 
TGGTCTCCTT CGCTGGATGG ACCAAACCCA GAAAAAGACC CCTCTGTGTT GATTAAGACT 
GCCATTCGTT GTTGTAAGGC CCTGACAGGC ATTGATCTAA GTGTGTGCAC ACAATGGTAC 
CGTTTTGCAG AGATTCGCTA CCATCGCCCT GAGGAGACCC ACAAGGGGCG TACAGTTCCA 
GCTCATGTGG AGACAGTGGT TTTATTTTTC CCGGATGTTT GGCATTGCCT TCCCACCCGC 
TCAGAGTGGG AAACCCTCTC CCGAGGATAC AAGCAGCAGC TGGTCGAGAA GCTTCAGGGT 
GAACGCAAGA AGGCTGATGG AGAACAGGAT GAAGAAGAGA AGGATGATGG TGAAGTTAAA 
GAAATTGCTA CTCCTACCCA TTGGTCTAAA CTTGATCCAA AGGCAATGAA GGTAAACGAT 
CTCCGAAAAG AATTAGAAAG TCGAGCTCTT AGTTCCAAAG GACTAAAATC GCAGTTAATA 
GCTCGCCTGA CAAAGCAGCT TAAAATAGAA GAACAGAAAG AAGAGCAGAA GGAATTAGAG 
AAGTCTGAAA AGGAAGAGGA AGATGAGGAT GATAAGAAGT CTGAGGATGA TAAAGAGGAA 
GAAGAAAGAA AACGTCAAGA AGAAGTGGAA CGCCAACGCC AAGAAAGAAG ATACATTTTG 
CCTGATGAGC CTGCCATAAT TGTCCATCCA AACTGGGCTG CAAAAAGTGG GAAGTTTGAT 
TGCAGCATCA TGTCTTTGAG TGTCCTTTTA GACTACAGAT TGGAAGATAA TAAAGAACAT 
TCGTTTGAGG TTTCATTGTT TGCAGAACTT TTCAATGAAA TGCTTCAAAG AGATTTTGGG 
GTCAGAATAT ACAAATCATT ACTCTCTCTT CCTGAGAAAG AGGACAAAAA AGATAAGGAA 
AAGAAAAGCA AAAAAGAAGA GAGAAAAGAT AAAAAAGAAG AGAGAGAAGA TGATATTGAT 
GAACCAAAAC CAAAACGGAG AAAATCGGGC GATGATAAAG ACAAAAAAGA AGACAGAGAT 
GAGAGGAAGA AAGAAGAGAA AAGAAAAGAT GATTCTAAGG ATGATGATGA AACTGAAGAA 
GATAACAATC AGGATGAGTA TGACCCCATG GAGGCAGAGG AGGCGGAGGA GGAGGAGGAC 
GCGGACCTGT CTCCCCTTTG CTTTACAGAC AGGGAGGAGG AGGAAGTGAA CAAACGAGAC 
GACAAAAGGG ACGTCAGCCG ATACTGCAAG GACAGACCCT CAAAGGACAA GGAAAAAGAG 
AAGCCTCAAA TGGTCACAGT TAACAGGGAT CTGCTAATGG CCTTTGTTTA TTTTGATCAA 
AGTCATTGTG GTTACCTTCT TGAAAAGGAT TTGGAAGAAA TACTTTATAC TCTTGGACTG 
CATCTTTCTC GGGCTCAGGT AAAGAAACTG CTCAATAAAG TAGTGCTCCG AGAATCGTGC 
TTCTATCGGA AATTAACAGA CACCTCAAAA GACGGCGAGA GCCATGACGA GCCAGAGTCG 
CTGCAGGGGG ACACGACGCT GGGAAACAGG TTACTACTTC CCACACCAAC AGTGAAGCAG 
GAATCGAAGG ATGGAGAAGA AAACGTAGGG CTTATTGTGT ACAACGGTGC AATGGTGGAT 
GTTGGGAGTC TCTTACAAAA GCTGGAAAAG AGTGAAAAAG TAAGAGCTGA GGTAGAACAG 
AAGCTCCAGT TACTAGAGGA GAAAACAGAT GAAGACGGAA AAACTATATT AAACTTGGAG 
AATTCTAACA AAAGCCTCTC TGGTGAACTT AGAGAGGTCA AAAAAGACCT TGGTCAATTA 
CAAGAAAACT TGAAGGTTTC AGAAAACATG AATTTGCAAT TTGAAAACCA ATTGAATAAA 
ACACTCAGAA ACTTATCCAC AGTCATGGAT GATATCCACA CTGTCCTAAA AAAGGATAAC 
GTAAAGAATG AAGACAGAGA TCAGAAATCC AAGGAGAATG GTTCAAGTGT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008771116.1
CDS	133..3606
Translation	MAQFGGQKNPPWATQFTATAVSQPAALGVQQPSLLGASPTIYTQQTALAAAGLTTQTPANYQLTQTAALQQQAAAVLQQQYSQPQQALYSVQQQLQQPQQTILTQPAVALPTSLSLSTPQPAAQITVSYPTPRSSQQQTQPQKQRVFTGVVTKLHDTFGFVDEDVFFQLGAVKGKTPQVGDRVLVEATYNPNMPFKWNAQRIQTLPNQNQSQTQPLLKTPTAVIQPIVPQTTFGVQAQPQPQSLLQAQISAASITPLLQTQPQPLLQQPQQKAGLLQPPVRIVSQPQPARRLDPPSRFSGRNDRGDQVPNRKDDRSRERDRERRRSRERSPQRKRSRERSPRRERERSPRRVRRVVPRYTVQFSKFSLDCPSCDMMELRRRYQNLYIPSDFFDAQFTWVDAFPLSRPFQLGNYCNFYVMHREVESLEKNVAVLDPPDADHLYSAKVMLMASPSMEDLYHKSCALAEDPQDLRDGFQHPARLVKFLVGMKGKDEAMAIGGHWSPSLDGPNPEKDPSVLIKTAIRCCKALTGIDLSVCTQWYRFAEIRYHRPEETHKGRTVPAHVETVVLFFPDVWHCLPTRSEWETLSRGYKQQLVEKLQGERKKADGEQDEEEKDDGEVKEIATPTHWSKLDPKAMKVNDLRKELESRALSSKGLKSQLIARLTKQLKIEEQKEEQKELEKSEKEEEDEDDKKSEDDKEEEERKRQEEVERQRQERRYILPDEPAIIVHPNWAAKSGKFDCSIMSLSVLLDYRLEDNKEHSFEVSLFAELFNEMLQRDFGVRIYKSLLSLPEKEDKKDKEKKSKKEERKDKKEEREDDIDEPKPKRRKSGDDKDKKEDRDERKKEEKRKDDSKDDDETEEDNNQDEYDPMEAEEAEEEEDADLSPLCFTDREEEEVNKRDDKRDVSRYCKDRPSKDKEKEKPQMVTVNRDLLMAFVYFDQSHCGYLLEKDLEEILYTLGLHLSRAQVKKLLNKVVLRESCFYRKLTDTSKDGESHDEPESLQGDTTLGNRLLLPTPTVKQESKDGEENVGLIVYNGAMVDVGSLLQKLEKSEKVRAEVEQKLQLLEEKTDEDGKTILNLENSNKSLSGELREVKKDLGQLQENLKVSENMNLQFENQLNKTLRNLSTVMDDIHTVLKKDNVKNEDRDQKSKENGSSV

Target ORF information:

RefSeq Version	XM_008772894.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cell division cycle and apoptosis regulator 1 (Ccar1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008772894.1

ORF Insert Sequence:

ATGGCTCAGT TTGGAGGACA GAAGAATCCT CCATGGGCTA CTCAGTTTAC AGCCACGGCG 
GTGTCACAAC CAGCTGCACT AGGTGTTCAG CAGCCATCAC TTCTGGGAGC ATCCCCTACC 
ATCTATACGC AGCAGACTGC ATTGGCGGCC GCAGGCCTTA CCACGCAGAC GCCAGCAAAC 
TATCAGTTAA CACAAACTGC GGCACTGCAG CAGCAAGCTG CAGCTGTCTT ACAGCAGCAA 
TATTCACAAC CTCAGCAGGC CTTGTATAGT GTGCAACAGC AGTTACAGCA ACCCCAGCAG 
ACCATTTTAA CACAGCCAGC TGTTGCATTG CCCACAAGCC TTAGCCTGTC TACTCCTCAG 
CCTGCAGCAC AGATTACTGT GTCCTATCCA ACACCAAGGT CCAGTCAACA GCAGACTCAA 
CCTCAGAAGC AGCGTGTTTT CACAGGAGTG GTTACAAAAC TACATGATAC GTTTGGATTT 
GTGGATGAAG ATGTATTCTT TCAGCTTGGT GCTGTTAAAG GGAAAACCCC CCAAGTGGGT 
GATAGAGTCT TGGTTGAAGC AACCTATAAT CCTAATATGC CTTTTAAATG GAATGCACAA 
AGAATTCAAA CACTACCAAA TCAGAATCAG TCACAAACCC AACCTTTACT GAAGACTCCT 
ACTGCTGTTA TCCAGCCGAT TGTGCCACAG ACAACATTTG GTGTTCAGGC ACAGCCCCAA 
CCCCAGTCAT TATTGCAAGC CCAGATCTCA GCTGCTTCTA TTACACCCCT ATTGCAGACG 
CAGCCACAGC CCTTACTACA GCAGCCACAG CAAAAAGCTG GCTTATTGCA GCCTCCTGTC 
CGAATAGTAT CACAGCCACA ACCCGCACGG AGATTAGATC CGCCATCTAG ATTTTCAGGA 
AGAAATGACA GAGGGGATCA AGTACCTAAT AGAAAAGATG ACCGAAGTCG TGAAAGGGAC 
AGAGAAAGAC GCAGATCCAG AGAAAGATCA CCTCAGAGGA AACGTTCCCG GGAGAGGTCG 
CCCCGGAGAG AAAGAGAGCG ATCCCCTCGG AGAGTCCGCC GTGTCGTTCC ACGGTACACA 
GTGCAGTTTT CAAAGTTTTC TTTAGATTGT CCCAGTTGTG ACATGATGGA ATTAAGGCGC 
CGTTATCAGA ACTTATATAT TCCTAGTGAC TTTTTTGATG CTCAGTTTAC ATGGGTGGAT 
GCTTTCCCTT TGTCAAGACC ATTTCAACTG GGAAATTACT GCAATTTTTA TGTGATGCAC 
CGAGAAGTGG AGTCCTTAGA AAAAAATGTG GCTGTTCTTG ACCCACCTGA TGCTGACCAC 
CTGTACAGTG CAAAGGTAAT GCTGATGGCT AGCCCTAGTA TGGAAGATTT GTATCATAAG 
TCATGTGCTC TTGCTGAAGA CCCACAAGAC CTTCGTGATG GTTTTCAGCA TCCTGCTAGA 
CTTGTTAAGT TTCTAGTGGG AATGAAAGGC AAGGATGAAG CCATGGCCAT TGGAGGCCAC 
TGGTCTCCTT CGCTGGATGG ACCAAACCCA GAAAAAGACC CCTCTGTGTT GATTAAGACT 
GCCATTCGTT GTTGTAAGGC CCTGACAGGC ATTGATCTAA GTGTGTGCAC ACAATGGTAC 
CGTTTTGCAG AGATTCGCTA CCATCGCCCT GAGGAGACCC ACAAGGGGCG TACAGTTCCA 
GCTCATGTGG AGACAGTGGT TTTATTTTTC CCGGATGTTT GGCATTGCCT TCCCACCCGC 
TCAGAGTGGG AAACCCTCTC CCGAGGATAC AAGCAGCAGC TGGTCGAGAA GCTTCAGGGT 
GAACGCAAGA AGGCTGATGG AGAACAGGAT GAAGAAGAGA AGGATGATGG TGAAGTTAAA 
GAAATTGCTA CTCCTACCCA TTGGTCTAAA CTTGATCCAA AGGCAATGAA GGTAAACGAT 
CTCCGAAAAG AATTAGAAAG TCGAGCTCTT AGTTCCAAAG GACTAAAATC GCAGTTAATA 
GCTCGCCTGA CAAAGCAGCT TAAAATAGAA GAACAGAAAG AAGAGCAGAA GGAATTAGAG 
AAGTCTGAAA AGGAAGAGGA AGATGAGGAT GATAAGAAGT CTGAGGATGA TAAAGAGGAA 
GAAGAAAGAA AACGTCAAGA AGAAGTGGAA CGCCAACGCC AAGAAAGAAG ATACATTTTG 
CCTGATGAGC CTGCCATAAT TGTCCATCCA AACTGGGCTG CAAAAAGTGG GAAGTTTGAT 
TGCAGCATCA TGTCTTTGAG TGTCCTTTTA GACTACAGAT TGGAAGATAA TAAAGAACAT 
TCGTTTGAGG TTTCATTGTT TGCAGAACTT TTCAATGAAA TGCTTCAAAG AGATTTTGGG 
GTCAGAATAT ACAAATCATT ACTCTCTCTT CCTGAGAAAG AGGACAAAAA AGATAAGGAA 
AAGAAAAGCA AAAAAGAAGA GAGAAAAGAT AAAAAAGAAG AGAGAGAAGA TGATATTGAT 
GAACCAAAAC CAAAACGGAG AAAATCGGGC GATGATAAAG ACAAAAAAGA AGACAGAGAT 
GAGAGGAAGA AAGAAGAGAA AAGAAAAGAT GATTCTAAGG ATGATGATGA AACTGAAGAA 
GATAACAATC AGGATGAGTA TGACCCCATG GAGGCAGAGG AGGCGGAGGA GGAGGAGGAC 
GCGGACCTGT CTCCCCTTTG CTTTACAGAC AGGGAGGAGG AGGAAGTGAA CAAACGAGAC 
GACAAAAGGG ACGTCAGCCG ATACTGCAAG GACAGACCCT CAAAGGACAA GGAAAAAGAG 
AAGCCTCAAA TGGTCACAGT TAACAGGGAT CTGCTAATGG CCTTTGTTTA TTTTGATCAA 
AGTCATTGTG GTTACCTTCT TGAAAAGGAT TTGGAAGAAA TACTTTATAC TCTTGGACTG 
CATCTTTCTC GGGCTCAGGT AAAGAAACTG CTCAATAAAG TAGTGCTCCG AGAATCGTGC 
TTCTATCGGA AATTAACAGA CACCTCAAAA GACGGCGAGA GCCATGACGA GCCAGAGTCG 
CTGCAGGGGG ACACGACGCT GGGAAACAGG TTACTACTTC CCACACCAAC AGTGAAGCAG 
GAATCGAAGG ATGGAGAAGA AAACGTAGGG CTTATTGTGT ACAACGGTGC AATGGTGGAT 
GTTGGGAGTC TCTTACAAAA GCTGGAAAAG AGTGAAAAAG TAAGAGCTGA GGTAGAACAG 
AAGCTCCAGT TACTAGAGGA GAAAACAGAT GAAGACGGAA AAACTATATT AAACTTGGAG 
AATTCTAACA AAAGCCTCTC TGGTGAACTT AGAGAGGTCA AAAAAGACCT TGGTCAATTA 
CAAGAAAACT TGAAGGTTTC AGAAAACATG AATTTGCAAT TTGAAAACCA ATTGAATAAA 
ACACTCAGAA ACTTATCCAC AGTCATGGAT GATATCCACA CTGTCCTAAA AAAGGATAAC 
GTAAAGAATG AAGACAGAGA TCAGAAATCC AAGGAGAATG GTTCAAGTGT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ccar1 ( XM_006256463.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006256525.1 Rattus norvegicus cell division cycle and apoptosis regulator 1 (Ccar1), transcript variant X2, mRNA.

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CloneID	ORa39466
Clone ID Related Accession (Same CDS sequence)	XM_006256462.2 , XM_008772894.1 , XM_006256463.2
Accession Version	XM_006256463.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3474bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	cell division cycle and apoptosis regulator protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005119.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006256463.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGT TTGGAGGACA GAAGAATCCT CCATGGGCTA CTCAGTTTAC AGCCACGGCG 
GTGTCACAAC CAGCTGCACT AGGTGTTCAG CAGCCATCAC TTCTGGGAGC ATCCCCTACC 
ATCTATACGC AGCAGACTGC ATTGGCGGCC GCAGGCCTTA CCACGCAGAC GCCAGCAAAC 
TATCAGTTAA CACAAACTGC GGCACTGCAG CAGCAAGCTG CAGCTGTCTT ACAGCAGCAA 
TATTCACAAC CTCAGCAGGC CTTGTATAGT GTGCAACAGC AGTTACAGCA ACCCCAGCAG 
ACCATTTTAA CACAGCCAGC TGTTGCATTG CCCACAAGCC TTAGCCTGTC TACTCCTCAG 
CCTGCAGCAC AGATTACTGT GTCCTATCCA ACACCAAGGT CCAGTCAACA GCAGACTCAA 
CCTCAGAAGC AGCGTGTTTT CACAGGAGTG GTTACAAAAC TACATGATAC GTTTGGATTT 
GTGGATGAAG ATGTATTCTT TCAGCTTGGT GCTGTTAAAG GGAAAACCCC CCAAGTGGGT 
GATAGAGTCT TGGTTGAAGC AACCTATAAT CCTAATATGC CTTTTAAATG GAATGCACAA 
AGAATTCAAA CACTACCAAA TCAGAATCAG TCACAAACCC AACCTTTACT GAAGACTCCT 
ACTGCTGTTA TCCAGCCGAT TGTGCCACAG ACAACATTTG GTGTTCAGGC ACAGCCCCAA 
CCCCAGTCAT TATTGCAAGC CCAGATCTCA GCTGCTTCTA TTACACCCCT ATTGCAGACG 
CAGCCACAGC CCTTACTACA GCAGCCACAG CAAAAAGCTG GCTTATTGCA GCCTCCTGTC 
CGAATAGTAT CACAGCCACA ACCCGCACGG AGATTAGATC CGCCATCTAG ATTTTCAGGA 
AGAAATGACA GAGGGGATCA AGTACCTAAT AGAAAAGATG ACCGAAGTCG TGAAAGGGAC 
AGAGAAAGAC GCAGATCCAG AGAAAGATCA CCTCAGAGGA AACGTTCCCG GGAGAGGTCG 
CCCCGGAGAG AAAGAGAGCG ATCCCCTCGG AGAGTCCGCC GTGTCGTTCC ACGGTACACA 
GTGCAGTTTT CAAAGTTTTC TTTAGATTGT CCCAGTTGTG ACATGATGGA ATTAAGGCGC 
CGTTATCAGA ACTTATATAT TCCTAGTGAC TTTTTTGATG CTCAGTTTAC ATGGGTGGAT 
GCTTTCCCTT TGTCAAGACC ATTTCAACTG GGAAATTACT GCAATTTTTA TGTGATGCAC 
CGAGAAGTGG AGTCCTTAGA AAAAAATGTG GCTGTTCTTG ACCCACCTGA TGCTGACCAC 
CTGTACAGTG CAAAGGTAAT GCTGATGGCT AGCCCTAGTA TGGAAGATTT GTATCATAAG 
TCATGTGCTC TTGCTGAAGA CCCACAAGAC CTTCGTGATG GTTTTCAGCA TCCTGCTAGA 
CTTGTTAAGT TTCTAGTGGG AATGAAAGGC AAGGATGAAG CCATGGCCAT TGGAGGCCAC 
TGGTCTCCTT CGCTGGATGG ACCAAACCCA GAAAAAGACC CCTCTGTGTT GATTAAGACT 
GCCATTCGTT GTTGTAAGGC CCTGACAGGC ATTGATCTAA GTGTGTGCAC ACAATGGTAC 
CGTTTTGCAG AGATTCGCTA CCATCGCCCT GAGGAGACCC ACAAGGGGCG TACAGTTCCA 
GCTCATGTGG AGACAGTGGT TTTATTTTTC CCGGATGTTT GGCATTGCCT TCCCACCCGC 
TCAGAGTGGG AAACCCTCTC CCGAGGATAC AAGCAGCAGC TGGTCGAGAA GCTTCAGGGT 
GAACGCAAGA AGGCTGATGG AGAACAGGAT GAAGAAGAGA AGGATGATGG TGAAGTTAAA 
GAAATTGCTA CTCCTACCCA TTGGTCTAAA CTTGATCCAA AGGCAATGAA GGTAAACGAT 
CTCCGAAAAG AATTAGAAAG TCGAGCTCTT AGTTCCAAAG GACTAAAATC GCAGTTAATA 
GCTCGCCTGA CAAAGCAGCT TAAAATAGAA GAACAGAAAG AAGAGCAGAA GGAATTAGAG 
AAGTCTGAAA AGGAAGAGGA AGATGAGGAT GATAAGAAGT CTGAGGATGA TAAAGAGGAA 
GAAGAAAGAA AACGTCAAGA AGAAGTGGAA CGCCAACGCC AAGAAAGAAG ATACATTTTG 
CCTGATGAGC CTGCCATAAT TGTCCATCCA AACTGGGCTG CAAAAAGTGG GAAGTTTGAT 
TGCAGCATCA TGTCTTTGAG TGTCCTTTTA GACTACAGAT TGGAAGATAA TAAAGAACAT 
TCGTTTGAGG TTTCATTGTT TGCAGAACTT TTCAATGAAA TGCTTCAAAG AGATTTTGGG 
GTCAGAATAT ACAAATCATT ACTCTCTCTT CCTGAGAAAG AGGACAAAAA AGATAAGGAA 
AAGAAAAGCA AAAAAGAAGA GAGAAAAGAT AAAAAAGAAG AGAGAGAAGA TGATATTGAT 
GAACCAAAAC CAAAACGGAG AAAATCGGGC GATGATAAAG ACAAAAAAGA AGACAGAGAT 
GAGAGGAAGA AAGAAGAGAA AAGAAAAGAT GATTCTAAGG ATGATGATGA AACTGAAGAA 
GATAACAATC AGGATGAGTA TGACCCCATG GAGGCAGAGG AGGCGGAGGA GGAGGAGGAC 
GCGGACCTGT CTCCCCTTTG CTTTACAGAC AGGGAGGAGG AGGAAGTGAA CAAACGAGAC 
GACAAAAGGG ACGTCAGCCG ATACTGCAAG GACAGACCCT CAAAGGACAA GGAAAAAGAG 
AAGCCTCAAA TGGTCACAGT TAACAGGGAT CTGCTAATGG CCTTTGTTTA TTTTGATCAA 
AGTCATTGTG GTTACCTTCT TGAAAAGGAT TTGGAAGAAA TACTTTATAC TCTTGGACTG 
CATCTTTCTC GGGCTCAGGT AAAGAAACTG CTCAATAAAG TAGTGCTCCG AGAATCGTGC 
TTCTATCGGA AATTAACAGA CACCTCAAAA GACGGCGAGA GCCATGACGA GCCAGAGTCG 
CTGCAGGGGG ACACGACGCT GGGAAACAGG TTACTACTTC CCACACCAAC AGTGAAGCAG 
GAATCGAAGG ATGGAGAAGA AAACGTAGGG CTTATTGTGT ACAACGGTGC AATGGTGGAT 
GTTGGGAGTC TCTTACAAAA GCTGGAAAAG AGTGAAAAAG TAAGAGCTGA GGTAGAACAG 
AAGCTCCAGT TACTAGAGGA GAAAACAGAT GAAGACGGAA AAACTATATT AAACTTGGAG 
AATTCTAACA AAAGCCTCTC TGGTGAACTT AGAGAGGTCA AAAAAGACCT TGGTCAATTA 
CAAGAAAACT TGAAGGTTTC AGAAAACATG AATTTGCAAT TTGAAAACCA ATTGAATAAA 
ACACTCAGAA ACTTATCCAC AGTCATGGAT GATATCCACA CTGTCCTAAA AAAGGATAAC 
GTAAAGAATG AAGACAGAGA TCAGAAATCC AAGGAGAATG GTTCAAGTGT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006256525.1
CDS	138..3611
Translation	MAQFGGQKNPPWATQFTATAVSQPAALGVQQPSLLGASPTIYTQQTALAAAGLTTQTPANYQLTQTAALQQQAAAVLQQQYSQPQQALYSVQQQLQQPQQTILTQPAVALPTSLSLSTPQPAAQITVSYPTPRSSQQQTQPQKQRVFTGVVTKLHDTFGFVDEDVFFQLGAVKGKTPQVGDRVLVEATYNPNMPFKWNAQRIQTLPNQNQSQTQPLLKTPTAVIQPIVPQTTFGVQAQPQPQSLLQAQISAASITPLLQTQPQPLLQQPQQKAGLLQPPVRIVSQPQPARRLDPPSRFSGRNDRGDQVPNRKDDRSRERDRERRRSRERSPQRKRSRERSPRRERERSPRRVRRVVPRYTVQFSKFSLDCPSCDMMELRRRYQNLYIPSDFFDAQFTWVDAFPLSRPFQLGNYCNFYVMHREVESLEKNVAVLDPPDADHLYSAKVMLMASPSMEDLYHKSCALAEDPQDLRDGFQHPARLVKFLVGMKGKDEAMAIGGHWSPSLDGPNPEKDPSVLIKTAIRCCKALTGIDLSVCTQWYRFAEIRYHRPEETHKGRTVPAHVETVVLFFPDVWHCLPTRSEWETLSRGYKQQLVEKLQGERKKADGEQDEEEKDDGEVKEIATPTHWSKLDPKAMKVNDLRKELESRALSSKGLKSQLIARLTKQLKIEEQKEEQKELEKSEKEEEDEDDKKSEDDKEEEERKRQEEVERQRQERRYILPDEPAIIVHPNWAAKSGKFDCSIMSLSVLLDYRLEDNKEHSFEVSLFAELFNEMLQRDFGVRIYKSLLSLPEKEDKKDKEKKSKKEERKDKKEEREDDIDEPKPKRRKSGDDKDKKEDRDERKKEEKRKDDSKDDDETEEDNNQDEYDPMEAEEAEEEEDADLSPLCFTDREEEEVNKRDDKRDVSRYCKDRPSKDKEKEKPQMVTVNRDLLMAFVYFDQSHCGYLLEKDLEEILYTLGLHLSRAQVKKLLNKVVLRESCFYRKLTDTSKDGESHDEPESLQGDTTLGNRLLLPTPTVKQESKDGEENVGLIVYNGAMVDVGSLLQKLEKSEKVRAEVEQKLQLLEEKTDEDGKTILNLENSNKSLSGELREVKKDLGQLQENLKVSENMNLQFENQLNKTLRNLSTVMDDIHTVLKKDNVKNEDRDQKSKENGSSV

Target ORF information:

RefSeq Version	XM_006256463.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cell division cycle and apoptosis regulator 1 (Ccar1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006256463.2

ORF Insert Sequence:

ATGGCTCAGT TTGGAGGACA GAAGAATCCT CCATGGGCTA CTCAGTTTAC AGCCACGGCG 
GTGTCACAAC CAGCTGCACT AGGTGTTCAG CAGCCATCAC TTCTGGGAGC ATCCCCTACC 
ATCTATACGC AGCAGACTGC ATTGGCGGCC GCAGGCCTTA CCACGCAGAC GCCAGCAAAC 
TATCAGTTAA CACAAACTGC GGCACTGCAG CAGCAAGCTG CAGCTGTCTT ACAGCAGCAA 
TATTCACAAC CTCAGCAGGC CTTGTATAGT GTGCAACAGC AGTTACAGCA ACCCCAGCAG 
ACCATTTTAA CACAGCCAGC TGTTGCATTG CCCACAAGCC TTAGCCTGTC TACTCCTCAG 
CCTGCAGCAC AGATTACTGT GTCCTATCCA ACACCAAGGT CCAGTCAACA GCAGACTCAA 
CCTCAGAAGC AGCGTGTTTT CACAGGAGTG GTTACAAAAC TACATGATAC GTTTGGATTT 
GTGGATGAAG ATGTATTCTT TCAGCTTGGT GCTGTTAAAG GGAAAACCCC CCAAGTGGGT 
GATAGAGTCT TGGTTGAAGC AACCTATAAT CCTAATATGC CTTTTAAATG GAATGCACAA 
AGAATTCAAA CACTACCAAA TCAGAATCAG TCACAAACCC AACCTTTACT GAAGACTCCT 
ACTGCTGTTA TCCAGCCGAT TGTGCCACAG ACAACATTTG GTGTTCAGGC ACAGCCCCAA 
CCCCAGTCAT TATTGCAAGC CCAGATCTCA GCTGCTTCTA TTACACCCCT ATTGCAGACG 
CAGCCACAGC CCTTACTACA GCAGCCACAG CAAAAAGCTG GCTTATTGCA GCCTCCTGTC 
CGAATAGTAT CACAGCCACA ACCCGCACGG AGATTAGATC CGCCATCTAG ATTTTCAGGA 
AGAAATGACA GAGGGGATCA AGTACCTAAT AGAAAAGATG ACCGAAGTCG TGAAAGGGAC 
AGAGAAAGAC GCAGATCCAG AGAAAGATCA CCTCAGAGGA AACGTTCCCG GGAGAGGTCG 
CCCCGGAGAG AAAGAGAGCG ATCCCCTCGG AGAGTCCGCC GTGTCGTTCC ACGGTACACA 
GTGCAGTTTT CAAAGTTTTC TTTAGATTGT CCCAGTTGTG ACATGATGGA ATTAAGGCGC 
CGTTATCAGA ACTTATATAT TCCTAGTGAC TTTTTTGATG CTCAGTTTAC ATGGGTGGAT 
GCTTTCCCTT TGTCAAGACC ATTTCAACTG GGAAATTACT GCAATTTTTA TGTGATGCAC 
CGAGAAGTGG AGTCCTTAGA AAAAAATGTG GCTGTTCTTG ACCCACCTGA TGCTGACCAC 
CTGTACAGTG CAAAGGTAAT GCTGATGGCT AGCCCTAGTA TGGAAGATTT GTATCATAAG 
TCATGTGCTC TTGCTGAAGA CCCACAAGAC CTTCGTGATG GTTTTCAGCA TCCTGCTAGA 
CTTGTTAAGT TTCTAGTGGG AATGAAAGGC AAGGATGAAG CCATGGCCAT TGGAGGCCAC 
TGGTCTCCTT CGCTGGATGG ACCAAACCCA GAAAAAGACC CCTCTGTGTT GATTAAGACT 
GCCATTCGTT GTTGTAAGGC CCTGACAGGC ATTGATCTAA GTGTGTGCAC ACAATGGTAC 
CGTTTTGCAG AGATTCGCTA CCATCGCCCT GAGGAGACCC ACAAGGGGCG TACAGTTCCA 
GCTCATGTGG AGACAGTGGT TTTATTTTTC CCGGATGTTT GGCATTGCCT TCCCACCCGC 
TCAGAGTGGG AAACCCTCTC CCGAGGATAC AAGCAGCAGC TGGTCGAGAA GCTTCAGGGT 
GAACGCAAGA AGGCTGATGG AGAACAGGAT GAAGAAGAGA AGGATGATGG TGAAGTTAAA 
GAAATTGCTA CTCCTACCCA TTGGTCTAAA CTTGATCCAA AGGCAATGAA GGTAAACGAT 
CTCCGAAAAG AATTAGAAAG TCGAGCTCTT AGTTCCAAAG GACTAAAATC GCAGTTAATA 
GCTCGCCTGA CAAAGCAGCT TAAAATAGAA GAACAGAAAG AAGAGCAGAA GGAATTAGAG 
AAGTCTGAAA AGGAAGAGGA AGATGAGGAT GATAAGAAGT CTGAGGATGA TAAAGAGGAA 
GAAGAAAGAA AACGTCAAGA AGAAGTGGAA CGCCAACGCC AAGAAAGAAG ATACATTTTG 
CCTGATGAGC CTGCCATAAT TGTCCATCCA AACTGGGCTG CAAAAAGTGG GAAGTTTGAT 
TGCAGCATCA TGTCTTTGAG TGTCCTTTTA GACTACAGAT TGGAAGATAA TAAAGAACAT 
TCGTTTGAGG TTTCATTGTT TGCAGAACTT TTCAATGAAA TGCTTCAAAG AGATTTTGGG 
GTCAGAATAT ACAAATCATT ACTCTCTCTT CCTGAGAAAG AGGACAAAAA AGATAAGGAA 
AAGAAAAGCA AAAAAGAAGA GAGAAAAGAT AAAAAAGAAG AGAGAGAAGA TGATATTGAT 
GAACCAAAAC CAAAACGGAG AAAATCGGGC GATGATAAAG ACAAAAAAGA AGACAGAGAT 
GAGAGGAAGA AAGAAGAGAA AAGAAAAGAT GATTCTAAGG ATGATGATGA AACTGAAGAA 
GATAACAATC AGGATGAGTA TGACCCCATG GAGGCAGAGG AGGCGGAGGA GGAGGAGGAC 
GCGGACCTGT CTCCCCTTTG CTTTACAGAC AGGGAGGAGG AGGAAGTGAA CAAACGAGAC 
GACAAAAGGG ACGTCAGCCG ATACTGCAAG GACAGACCCT CAAAGGACAA GGAAAAAGAG 
AAGCCTCAAA TGGTCACAGT TAACAGGGAT CTGCTAATGG CCTTTGTTTA TTTTGATCAA 
AGTCATTGTG GTTACCTTCT TGAAAAGGAT TTGGAAGAAA TACTTTATAC TCTTGGACTG 
CATCTTTCTC GGGCTCAGGT AAAGAAACTG CTCAATAAAG TAGTGCTCCG AGAATCGTGC 
TTCTATCGGA AATTAACAGA CACCTCAAAA GACGGCGAGA GCCATGACGA GCCAGAGTCG 
CTGCAGGGGG ACACGACGCT GGGAAACAGG TTACTACTTC CCACACCAAC AGTGAAGCAG 
GAATCGAAGG ATGGAGAAGA AAACGTAGGG CTTATTGTGT ACAACGGTGC AATGGTGGAT 
GTTGGGAGTC TCTTACAAAA GCTGGAAAAG AGTGAAAAAG TAAGAGCTGA GGTAGAACAG 
AAGCTCCAGT TACTAGAGGA GAAAACAGAT GAAGACGGAA AAACTATATT AAACTTGGAG 
AATTCTAACA AAAGCCTCTC TGGTGAACTT AGAGAGGTCA AAAAAGACCT TGGTCAATTA 
CAAGAAAACT TGAAGGTTTC AGAAAACATG AATTTGCAAT TTGAAAACCA ATTGAATAAA 
ACACTCAGAA ACTTATCCAC AGTCATGGAT GATATCCACA CTGTCCTAAA AAAGGATAAC 
GTAAAGAATG AAGACAGAGA TCAGAAATCC AAGGAGAATG GTTCAAGTGT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ccar1 ( XM_006256464.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006256526.1 Rattus norvegicus cell division cycle and apoptosis regulator 1 (Ccar1), transcript variant X4, mRNA.

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CloneID	ORa39467
Clone ID Related Accession (Same CDS sequence)	XM_006256464.3
Accession Version	XM_006256464.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3288bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	cell division cycle and apoptosis regulator protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005119.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006256464.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGT TTGGAGGACA GAAGAATCCT CCATGGGCTA CTCAGTTTAC AGCCACGGCG 
GTGTCACAAC CAGCTGCACT AGGTGTTCAG CAGCCATCAC TTCTGGGAGC ATCCCCTACC 
ATCTATACGC AGCAGACTGC ATTGGCGGCC GCAGGCCTTA CCACGCAGAC GCCAGCAAAC 
TATCAGTTAA CACAAACTGC GGCACTGCAG CAGCAAGCTG CAGCTGTCTT ACAGCAGCAA 
TATTCACAAC CTCAGCAGGC CTTGTATAGT GTGCAACAGC AGTTACAGCA ACCCCAGCAG 
ACCATTTTAA CACAGCCAGC TGTTGCATTG CCCACAAGCC TTAGCCTGTC TACTCCTCAG 
CCTGCAGCAC AGATTACTGT GTCCTATCCA ACACCAAGGT CCAGTCAACA GCAGACTCAA 
CCTCAGAAGC AGCGTGTTTT CACAGGAGTG GTTACAAAAC TACATGATAC GTTTGGATTT 
GTGGATGAAG ATGTATTCTT TCAGCTTGGT GCTGTTAAAG GGAAAACCCC CCAAGTGGGT 
GATAGAGTCT TGGTTGAAGC AACCTATAAT CCTAATATGC CTTTTAAATG GAATGCACAA 
AGAATTCAAA CACTACCAAA TCAGAATCAG TCACAAACCC AACCTTTACT GAAGACTCCT 
ACTGCTGTTA TCCAGCCGAT TGTGCCACAG ACAACATTTG GTGTTCAGGC ACAGCCCCAA 
CCCCAGTCAT TATTGCAAGC CCAGATCTCA GCTGCTTCTA TTACACCCCT ATTGCAGACG 
CAGCCACAGC CCTTACTACA GCAGCCACAG CAAAAAGCTG GCTTATTGCA GCCTCCTGTC 
CGAATAGTAT CACAGCCACA ACCCGCACGG AGATTAGATC CGCCATCTAG ATTTTCAGGA 
AGAAATGACA GAGGGGATCA AGTACCTAAT AGAAAAGATG ACCGAAGTCG TGAAAGGGAC 
AGAGAAAGAC GCAGATCCAG AGAAAGATCA CCTCAGAGGA AACGTTCCCG GGAGAGGTCG 
CCCCGGAGAG AAAGAGAGCG ATCCCCTCGG AGAGTCCGCC GTGTCGTTCC ACGGTACACA 
GTGCAGTTTT CAAAGTTTTC TTTAGATTGT CCCAGTTGTG ACATGATGGA ATTAAGGCGC 
CGTTATCAGA ACTTATATAT TCCTAGTGAC TTTTTTGATG CTCAGTTTAC ATGGGTGGAT 
GCTTTCCCTT TGTCAAGACC ATTTCAACTG GGAAATTACT GCAATTTTTA TGTGATGCAC 
CGAGAAGTGG AGTCCTTAGA AAAAAATGTG GCTGTTCTTG ACCCACCTGA TGCTGACCAC 
CTGTACAGTG CAAAGGTAAT GCTGATGGCT AGCCCTAGTA TGGAAGATTT GTATCATAAG 
TCATGTGCTC TTGCTGAAGA CCCACAAGAC CTTCGTGATG GTTTTCAGCA TCCTGCTAGA 
CTTGTTAAGT TTCTAGTGGG AATGAAAGGC AAGGATGAAG CCATGGCCAT TGGAGGCCAC 
TGGTCTCCTT CGCTGGATGG ACCAAACCCA GAAAAAGACC CCTCTGTGTT GATTAAGACT 
GCCATTCGTT GTTGTAAGGC CCTGACAGGC ATTGATCTAA GTGTGTGCAC ACAATGGTAC 
CGTTTTGCAG AGATTCGCTA CCATCGCCCT GAGGAGACCC ACAAGGGGCG TACAGTTCCA 
GCTCATGTGG AGACAGTGGT TTTATTTTTC CCGGATGTTT GGCATTGCCT TCCCACCCGC 
TCAGAGTGGG AAACCCTCTC CCGAGGATAC AAGCAGCAGC TGGTCGAGAA GCTTCAGGGT 
GAACGCAAGA AGGCTGATGG AGAACAGGAT GAAGAAGAGA AGGATGATGG TGAAGTTAAA 
GAAATTGCTA CTCCTACCCA TTGGTCTAAA CTTGATCCAA AGGCAATGAA GGAAGAAGAA 
AGAAAACGTC AAGAAGAAGT GGAACGCCAA CGCCAAGAAA GAAGATACAT TTTGCCTGAT 
GAGCCTGCCA TAATTGTCCA TCCAAACTGG GCTGCAAAAA GTGGGAAGTT TGATTGCAGC 
ATCATGTCTT TGAGTGTCCT TTTAGACTAC AGATTGGAAG ATAATAAAGA ACATTCGTTT 
GAGGTTTCAT TGTTTGCAGA ACTTTTCAAT GAAATGCTTC AAAGAGATTT TGGGGTCAGA 
ATATACAAAT CATTACTCTC TCTTCCTGAG AAAGAGGACA AAAAAGATAA GGAAAAGAAA 
AGCAAAAAAG AAGAGAGAAA AGATAAAAAA GAAGAGAGAG AAGATGATAT TGATGAACCA 
AAACCAAAAC GGAGAAAATC GGGCGATGAT AAAGACAAAA AAGAAGACAG AGATGAGAGG 
AAGAAAGAAG AGAAAAGAAA AGATGATTCT AAGGATGATG ATGAAACTGA AGAAGATAAC 
AATCAGGATG AGTATGACCC CATGGAGGCA GAGGAGGCGG AGGAGGAGGA GGACGCGGAC 
CTGTCTCCCC TTTGCTTTAC AGACAGGGAG GAGGAGGAAG TGAACAAACG AGACGACAAA 
AGGGACGTCA GCCGATACTG CAAGGACAGA CCCTCAAAGG ACAAGGAAAA AGAGAAGCCT 
CAAATGGTCA CAGTTAACAG GGATCTGCTA ATGGCCTTTG TTTATTTTGA TCAAAGTCAT 
TGTGGTTACC TTCTTGAAAA GGATTTGGAA GAAATACTTT ATACTCTTGG ACTGCATCTT 
TCTCGGGCTC AGGTAAAGAA ACTGCTCAAT AAAGTAGTGC TCCGAGAATC GTGCTTCTAT 
CGGAAATTAA CAGACACCTC AAAAGACGGC GAGAGCCATG ACGAGCCAGA GTCGCTGCAG 
GGGGACACGA CGCTGGGAAA CAGGTTACTA CTTCCCACAC CAACAGTGAA GCAGGAATCG 
AAGGATGGAG AAGAAAACGT AGGGCTTATT GTGTACAACG GTGCAATGGT GGATGTTGGG 
AGTCTCTTAC AAAAGCTGGA AAAGAGTGAA AAAGTAAGAG CTGAGGTAGA ACAGAAGCTC 
CAGTTACTAG AGGAGAAAAC AGATGAAGAC GGAAAAACTA TATTAAACTT GGAGAATTCT 
AACAAAAGCC TCTCTGGTGA ACTTAGAGAG GTCAAAAAAG ACCTTGGTCA ATTACAAGAA 
AACTTGAAGG TTTCAGAAAA CATGAATTTG CAATTTGAAA ACCAATTGAA TAAAACACTC 
AGAAACTTAT CCACAGTCAT GGATGATATC CACACTGTCC TAAAAAAGGA TAACGTAAAG 
AATGAAGACA GAGATCAGAA ATCCAAGGAG AATGGTTCAA GTGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006256526.1
CDS	142..3429
Translation	MAQFGGQKNPPWATQFTATAVSQPAALGVQQPSLLGASPTIYTQQTALAAAGLTTQTPANYQLTQTAALQQQAAAVLQQQYSQPQQALYSVQQQLQQPQQTILTQPAVALPTSLSLSTPQPAAQITVSYPTPRSSQQQTQPQKQRVFTGVVTKLHDTFGFVDEDVFFQLGAVKGKTPQVGDRVLVEATYNPNMPFKWNAQRIQTLPNQNQSQTQPLLKTPTAVIQPIVPQTTFGVQAQPQPQSLLQAQISAASITPLLQTQPQPLLQQPQQKAGLLQPPVRIVSQPQPARRLDPPSRFSGRNDRGDQVPNRKDDRSRERDRERRRSRERSPQRKRSRERSPRRERERSPRRVRRVVPRYTVQFSKFSLDCPSCDMMELRRRYQNLYIPSDFFDAQFTWVDAFPLSRPFQLGNYCNFYVMHREVESLEKNVAVLDPPDADHLYSAKVMLMASPSMEDLYHKSCALAEDPQDLRDGFQHPARLVKFLVGMKGKDEAMAIGGHWSPSLDGPNPEKDPSVLIKTAIRCCKALTGIDLSVCTQWYRFAEIRYHRPEETHKGRTVPAHVETVVLFFPDVWHCLPTRSEWETLSRGYKQQLVEKLQGERKKADGEQDEEEKDDGEVKEIATPTHWSKLDPKAMKEEERKRQEEVERQRQERRYILPDEPAIIVHPNWAAKSGKFDCSIMSLSVLLDYRLEDNKEHSFEVSLFAELFNEMLQRDFGVRIYKSLLSLPEKEDKKDKEKKSKKEERKDKKEEREDDIDEPKPKRRKSGDDKDKKEDRDERKKEEKRKDDSKDDDETEEDNNQDEYDPMEAEEAEEEEDADLSPLCFTDREEEEVNKRDDKRDVSRYCKDRPSKDKEKEKPQMVTVNRDLLMAFVYFDQSHCGYLLEKDLEEILYTLGLHLSRAQVKKLLNKVVLRESCFYRKLTDTSKDGESHDEPESLQGDTTLGNRLLLPTPTVKQESKDGEENVGLIVYNGAMVDVGSLLQKLEKSEKVRAEVEQKLQLLEEKTDEDGKTILNLENSNKSLSGELREVKKDLGQLQENLKVSENMNLQFENQLNKTLRNLSTVMDDIHTVLKKDNVKNEDRDQKSKENGSSV

Target ORF information:

RefSeq Version	XM_006256464.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cell division cycle and apoptosis regulator 1 (Ccar1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006256464.3

ORF Insert Sequence:

ATGGCTCAGT TTGGAGGACA GAAGAATCCT CCATGGGCTA CTCAGTTTAC AGCCACGGCG 
GTGTCACAAC CAGCTGCACT AGGTGTTCAG CAGCCATCAC TTCTGGGAGC ATCCCCTACC 
ATCTATACGC AGCAGACTGC ATTGGCGGCC GCAGGCCTTA CCACGCAGAC GCCAGCAAAC 
TATCAGTTAA CACAAACTGC GGCACTGCAG CAGCAAGCTG CAGCTGTCTT ACAGCAGCAA 
TATTCACAAC CTCAGCAGGC CTTGTATAGT GTGCAACAGC AGTTACAGCA ACCCCAGCAG 
ACCATTTTAA CACAGCCAGC TGTTGCATTG CCCACAAGCC TTAGCCTGTC TACTCCTCAG 
CCTGCAGCAC AGATTACTGT GTCCTATCCA ACACCAAGGT CCAGTCAACA GCAGACTCAA 
CCTCAGAAGC AGCGTGTTTT CACAGGAGTG GTTACAAAAC TACATGATAC GTTTGGATTT 
GTGGATGAAG ATGTATTCTT TCAGCTTGGT GCTGTTAAAG GGAAAACCCC CCAAGTGGGT 
GATAGAGTCT TGGTTGAAGC AACCTATAAT CCTAATATGC CTTTTAAATG GAATGCACAA 
AGAATTCAAA CACTACCAAA TCAGAATCAG TCACAAACCC AACCTTTACT GAAGACTCCT 
ACTGCTGTTA TCCAGCCGAT TGTGCCACAG ACAACATTTG GTGTTCAGGC ACAGCCCCAA 
CCCCAGTCAT TATTGCAAGC CCAGATCTCA GCTGCTTCTA TTACACCCCT ATTGCAGACG 
CAGCCACAGC CCTTACTACA GCAGCCACAG CAAAAAGCTG GCTTATTGCA GCCTCCTGTC 
CGAATAGTAT CACAGCCACA ACCCGCACGG AGATTAGATC CGCCATCTAG ATTTTCAGGA 
AGAAATGACA GAGGGGATCA AGTACCTAAT AGAAAAGATG ACCGAAGTCG TGAAAGGGAC 
AGAGAAAGAC GCAGATCCAG AGAAAGATCA CCTCAGAGGA AACGTTCCCG GGAGAGGTCG 
CCCCGGAGAG AAAGAGAGCG ATCCCCTCGG AGAGTCCGCC GTGTCGTTCC ACGGTACACA 
GTGCAGTTTT CAAAGTTTTC TTTAGATTGT CCCAGTTGTG ACATGATGGA ATTAAGGCGC 
CGTTATCAGA ACTTATATAT TCCTAGTGAC TTTTTTGATG CTCAGTTTAC ATGGGTGGAT 
GCTTTCCCTT TGTCAAGACC ATTTCAACTG GGAAATTACT GCAATTTTTA TGTGATGCAC 
CGAGAAGTGG AGTCCTTAGA AAAAAATGTG GCTGTTCTTG ACCCACCTGA TGCTGACCAC 
CTGTACAGTG CAAAGGTAAT GCTGATGGCT AGCCCTAGTA TGGAAGATTT GTATCATAAG 
TCATGTGCTC TTGCTGAAGA CCCACAAGAC CTTCGTGATG GTTTTCAGCA TCCTGCTAGA 
CTTGTTAAGT TTCTAGTGGG AATGAAAGGC AAGGATGAAG CCATGGCCAT TGGAGGCCAC 
TGGTCTCCTT CGCTGGATGG ACCAAACCCA GAAAAAGACC CCTCTGTGTT GATTAAGACT 
GCCATTCGTT GTTGTAAGGC CCTGACAGGC ATTGATCTAA GTGTGTGCAC ACAATGGTAC 
CGTTTTGCAG AGATTCGCTA CCATCGCCCT GAGGAGACCC ACAAGGGGCG TACAGTTCCA 
GCTCATGTGG AGACAGTGGT TTTATTTTTC CCGGATGTTT GGCATTGCCT TCCCACCCGC 
TCAGAGTGGG AAACCCTCTC CCGAGGATAC AAGCAGCAGC TGGTCGAGAA GCTTCAGGGT 
GAACGCAAGA AGGCTGATGG AGAACAGGAT GAAGAAGAGA AGGATGATGG TGAAGTTAAA 
GAAATTGCTA CTCCTACCCA TTGGTCTAAA CTTGATCCAA AGGCAATGAA GGAAGAAGAA 
AGAAAACGTC AAGAAGAAGT GGAACGCCAA CGCCAAGAAA GAAGATACAT TTTGCCTGAT 
GAGCCTGCCA TAATTGTCCA TCCAAACTGG GCTGCAAAAA GTGGGAAGTT TGATTGCAGC 
ATCATGTCTT TGAGTGTCCT TTTAGACTAC AGATTGGAAG ATAATAAAGA ACATTCGTTT 
GAGGTTTCAT TGTTTGCAGA ACTTTTCAAT GAAATGCTTC AAAGAGATTT TGGGGTCAGA 
ATATACAAAT CATTACTCTC TCTTCCTGAG AAAGAGGACA AAAAAGATAA GGAAAAGAAA 
AGCAAAAAAG AAGAGAGAAA AGATAAAAAA GAAGAGAGAG AAGATGATAT TGATGAACCA 
AAACCAAAAC GGAGAAAATC GGGCGATGAT AAAGACAAAA AAGAAGACAG AGATGAGAGG 
AAGAAAGAAG AGAAAAGAAA AGATGATTCT AAGGATGATG ATGAAACTGA AGAAGATAAC 
AATCAGGATG AGTATGACCC CATGGAGGCA GAGGAGGCGG AGGAGGAGGA GGACGCGGAC 
CTGTCTCCCC TTTGCTTTAC AGACAGGGAG GAGGAGGAAG TGAACAAACG AGACGACAAA 
AGGGACGTCA GCCGATACTG CAAGGACAGA CCCTCAAAGG ACAAGGAAAA AGAGAAGCCT 
CAAATGGTCA CAGTTAACAG GGATCTGCTA ATGGCCTTTG TTTATTTTGA TCAAAGTCAT 
TGTGGTTACC TTCTTGAAAA GGATTTGGAA GAAATACTTT ATACTCTTGG ACTGCATCTT 
TCTCGGGCTC AGGTAAAGAA ACTGCTCAAT AAAGTAGTGC TCCGAGAATC GTGCTTCTAT 
CGGAAATTAA CAGACACCTC AAAAGACGGC GAGAGCCATG ACGAGCCAGA GTCGCTGCAG 
GGGGACACGA CGCTGGGAAA CAGGTTACTA CTTCCCACAC CAACAGTGAA GCAGGAATCG 
AAGGATGGAG AAGAAAACGT AGGGCTTATT GTGTACAACG GTGCAATGGT GGATGTTGGG 
AGTCTCTTAC AAAAGCTGGA AAAGAGTGAA AAAGTAAGAG CTGAGGTAGA ACAGAAGCTC 
CAGTTACTAG AGGAGAAAAC AGATGAAGAC GGAAAAACTA TATTAAACTT GGAGAATTCT 
AACAAAAGCC TCTCTGGTGA ACTTAGAGAG GTCAAAAAAG ACCTTGGTCA ATTACAAGAA 
AACTTGAAGG TTTCAGAAAA CATGAATTTG CAATTTGAAA ACCAATTGAA TAAAACACTC 
AGAAACTTAT CCACAGTCAT GGATGATATC CACACTGTCC TAAAAAAGGA TAACGTAAAG 
AATGAAGACA GAGATCAGAA ATCCAAGGAG AATGGTTCAA GTGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

CCAR1/CoCoA pair-mediated recruitment of the Mediator defines a novel pathway for GATA1 function.
Genes to cells : devoted to molecular & cellular mechanisms19(1)28-51(2014 Jan)
Mizuta S,Minami T,Fujita H,Kaminaga C,Matsui K,Ishino R,Fujita A,Oda K,Kawai A,Hasegawa N,Urahama N,Roeder RG,Ito M

CCAR1 promotes chromatin loading of androgen receptor (AR) transcription complex by stabilizing the association between AR and GATA2.
Nucleic acids research41(18)8526-36(2013 Oct)
Seo WY,Jeong BC,Yu EJ,Kim HJ,Kim SH,Lim JE,Kwon GY,Lee HM,Kim JH

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanhäusser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

CCAR1, a key regulator of mediator complex recruitment to nuclear receptor transcription complexes.
Molecular cell31(4)510-9(2008 Aug)
Kim JH,Yang CK,Heo K,Roeder RG,An W,Stallcup MR

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Ccar1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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