Spink5 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Spink5
Entrez Gene ID	361319
Full Name	serine peptidase inhibitor, Kazal type 5
General protein information	Preferred Names serine peptidase inhibitor, Kazal type 5 Names serine peptidase inhibitor, Kazal type 5 serine protease inhibitor, Kazal type 5
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 18 Map Location: 18p11

mRNA and Protein(s)

mRNA	Protein	Name
NM_001170606.1	NP_001164077.1	serine peptidase inhibitor, Kazal type 5 precursor
XM_017600996.1	XP_017456485.1	serine peptidase inhibitor, Kazal type 5 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SPINK5	XP_001160618.1
Macaca mulatta (Rhesus monkey)	SPINK5	XP_001103296.1
Rattus norvegicus (Norway rat)	Spink5	NP_001164077.1
Canis lupus familiaris (dog)	SPINK5	NP_001020568.1
Gallus gallus (chicken)	SPINK7	NP_001106132.1
Rattus norvegicus (Norway rat)	LOC100910851	XP_003749120.1
Homo sapiens (human)	SPINK5	NP_001121170.1
Bos taurus (cattle)	SPINK5	NP_001095572.1
Gallus gallus (chicken)	SPINK5	XP_004944964.1
Mus musculus (house mouse)	Spink5	NP_001074649.1

Gene Ontology
Bibliography

Related articles in PubMed

Elastase 2 is expressed in human and mouse epidermis and impairs skin barrier function in Netherton syndrome through filaggrin and lipid misprocessing.
Bonnart C, Deraison C, Lacroix M, Uchida Y, Besson C, Robin A, Briot A, Gonthier M, Lamant L, Dubus P, Monsarrat B, Hovnanian A
The Journal of clinical investigation120(3)871-82(2010 Mar)

Kallikrein-mediated proteolysis regulates the antimicrobial effects of cathelicidins in skin.
Yamasaki K, Schauber J, Coda A, Lin H, Dorschner RA, Schechter NM, Bonnart C, Descargues P, Hovnanian A, Gallo RL
FASEB journal : official publication of the Federation of American Societies for Experimental Biology20(12)2068-80(2006 Oct)

Consequences of C-terminal domains and N-terminal signal peptide deletions on LEKTI secretion, stability, and subcellular distribution.
Jayakumar A, Kang Y, Henderson Y, Mitsudo K, Liu X, Briggs K, Wang M, Frederick MJ, El-Naggar AK, Bebök Z, Clayman GL
Archives of biochemistry and biophysics435(1)89-102(2005 Mar)

LEKTI is localized in lamellar granules, separated from KLK5 and KLK7, and is secreted in the extracellular spaces of the superficial stratum granulosum.
Ishida-Yamamoto A, Deraison C, Bonnart C, Bitoun E, Robinson R, O'Brien TJ, Wakamatsu K, Ohtsubo S, Takahashi H, Hashimoto Y, Dopping-Hepenstal PJ, McGrath JA, Iizuka H, Richard G, Hovnanian A
The Journal of investigative dermatology124(2)360-6(2005 Feb)

Epidermal detachment, desmosomal dissociation, and destabilization of corneodesmosin in Spink5-/- mice.
Yang T, Liang D, Koch PJ, Hohl D, Kheradmand F, Overbeek PA
Genes & development18(19)2354-8(2004 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Spink5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Spink5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa06383	NM_001170606.1 Latest version!	Rattus norvegicus serine peptidase inhibitor, Kazal type 5 (Spink5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa06383	XM_017600996.1 Latest version!	Rattus norvegicus serine peptidase inhibitor, Kazal type 5 (Spink5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Spink5 ( NM_001170606.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001164077.1 Rattus norvegicus serine peptidase inhibitor, Kazal type 5 (Spink5), mRNA.

Price & Availability↑

CloneID	ORa06383
Clone ID Related Accession (Same CDS sequence)	NM_001170606.1 , XM_017600996.1
Accession Version	NM_001170606.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3060bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1506787200000
Organism	Rattus norvegicus(Norway rat)
Product	serine peptidase inhibitor, Kazal type 5 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07031808.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2689602 [ECO:0000348] ##Evidence-Data-END##

ATGAAGACAG CCACGGTGCC AATGCTTCTC ACCCTGGCAT TTTATCTCAC ACAAGATGCC 
TCCAGTTGGA AAGGAAATCA GGACCCATGC ATGAAATTTC GAGCACTGAT GAAAAATGGG 
ACACTGTCAT GTCCACAAGG CAACAATTCT TCTCAAAGTC TCAATGACAT TATTTTCCAA 
AGTGAATGCA TCTTGTGCAA AAAAACACTA GAACAAGAAA CAAATACCAA GGACATGGTG 
ATGGTGAGGT TTTTGTCAAG AGCCACCAGG GCCACTGCCC CAGCAAAGTT GAATTGTGAG 
AACTTCAAGC AAAGAAGAAA AGATGGGGAT TTCATCTGTC CATCTGATGC TTCTGCAGTT 
TGTGGCACAG ATGGGAAAAC GTACCCCAGC AGATGTCACC TGTGTGCTGA GAATGCGAAA 
AGCCAGAGCC ATGTTGAAGT AAAAAGTGAA GGTGAATGTG GCAGCAGCCA CCTAGAGACG 
GATATGTGCA GTGATTTTCG GGCCTACGTG CAAGATGGAA GACTCGGATG TACAAGGGAA 
AATGATCCAA TCCGTGGTCC TGATGGGAGG ACACATGGCA ATAGGTGTGC CATGTGTGCT 
GAGCTCTTTT TAAAAGAAGC TCAAGTAAAT GCCACACGTG ACAGAGAAAG CAGAATTCGG 
AGAGATGCCG AGGAGGAACT TTGCAAGGAA TTTGAAAACC AAGTGAGGAG TGGAAGACTC 
TTTTGTACCC GAGAAAGTGA CCCAATCCGT GGCCCTGATG GCAAGATGCA TGGGAACAAG 
TGTGCCCTCT GTGCTGAGAT ATTCATGAAG CGTTTTACAG AAGGAAAAGA TAAAGCAGAG 
ACAAAACAGA GAGATGCTGA AGAAAGGGCT AAAGCTAAAA TGGAAATTAG GAAACGATGC 
AGTGAATTCC AGGATCGTGC AAAGAATGGA ACACTGTTCT GTACCAGAGA AAATGATCCT 
ATTCGTGGTC TAGATGGAAA AACACATGGC AACTTGTGTT CCATGTGCCA AGCTTTCTTC 
AAAACAGAAG CTGAAGAAAA GAAGGCTGAA GCTGGAACCA GAAACAGAAG AGGGTCTGAA 
AAATCAGAAA CATATGCAAA GCTATGTGAT GAATTTCGGA AGGTCAGAAA GAATGGACAA 
CTATATTGCA CCAGAGAGAA TGACCCAGTC AGGGGTCCTG ATGGGAAAAT CCATGGGAAC 
ATATGCTCCA TGTGTCAGTC TTTCTTTATT CAAGAAGACA AAGCAAGAGC AAAGGTTAAA 
AGAGAAGCTG CAAAGGAAAT GTGCAGTGAG TTCCAGGACC AAGCAAAGAA TGGAATGCTC 
ATTTGCACCA GAGAAAATGA CCCTGTTGTT GGCCCAGATG GAAAAACACA TGCAAACAAG 
TGTGCAATGT GTGCAAGTGT GTTTCTACTA GAAGAAGAAG AGAAGAAAAA TGATGACAAA 
GGGGAAAGGG AGAAGGCTGA GGCTGGAAAG ACGAAGAGAG AAGCAGTGCA GAATCTATGT 
AGTAAATATC GTACCTTGCT AAAAAATGGG CGGCTCCCCT GCACCAAAAA GAATGACCCC 
ATTGAGGGCC TGGATGGGAA GATCTATGCA AGTACCTGCT CCATGTGTGA GGCCTTCTTC 
CACCAAGAAG CGAAGAAAAA TGAAGCTGGC TTCGGAGCAA AGGCTAAAAG AGAAGTTAAG 
GTTGATTGCA GTGAATTTCT GACTCTTTTA AGAGGTGGAG AACTCTTTTG CACACGAGAA 
AATGACCCTG TGCGTGGCCC AGATGGCAAG ACCCATGGCA ACAAGTGTGC CATGTGCAAG 
GCTGTCTTTG AGAAAGAAAA TGAAGAAAGG AAGAGGAAAG AAGGGGAAAA CCAGAGAATT 
GCTGCAGGGC AGAGTTCCGG TGGTGGGAAC CAGAAAGCTA AGGATGAATG TGCTCAATAT 
TGGGAAAGCA TGAAAAATGG AGAACTTAGC TGTACTCGGG AGAGTGACCC TGTACGTGGT 
GCTGATGGGA AACCATACAA CAATAAATGT GTCATGTGTA AAGAACTACT GCGGAGAGAA 
ATGGAGGAAA AAAATAAAAA TTCTGCCTCA AGGTCAAATG GGACTGAAAC AAGAAAGGAT 
GTATGCGACC AGTTTAGAAA CCAAATGAAA AATGGGAAAC TCCTGTGCAC TCGGGAAAGC 
GACCCTACCC GGGGTCCAGA TGGCAAGACC CATGGCAATA AGTGTGCCAT GTGCAAGGAG 
AGATTGGAAA GAGAAGCTGC TGAAAAGAAA AAGAAAGAGG ATGAAGACAA GAGAAACACA 
GGAGCAAACA AGAGCAATAA GAAGGATCAG TGTCATGAAT TCCGGAGCAT GCAGCTAAAT 
GGAAGGCTGA TCTGCACCCG AGAAAATGAT CCCATTCGAG GTCCAGATGG CAAGACACAT 
GTAAACAAGT GTGCCATGTG TCAGATGATG TTTGAGCATG AAGCCAGTGA AAGAAAAATG 
AGACATGAAG AAAACTCACG CTCCCAGTCC ACAAATGAGG CAGAGGACAA CTGTGGAGAA 
GTTCATAACA CAGCACAGGA TGTCAAACCC AGGCAGGCCA GGAGTTCTTT GCCCTCCATA 
CGTGGGATCA GTGAAGATGA GTGCAGCAAT TTTCAAAATC TCATAAGTAA TGGAAAGCTT 
ATCTGTCCAG AAATGGATGA TCCATTGCAT GGTGCTGATG GAAGCTTTTA TCAGAACAAG 
TGCAACATGT GCAGAGATGT CCTTGAAAAA AAAGCTTTAG AAAGGTCAGG ATTTCAAGAA 
AAGTCATCAC AAATTAGATC TACTAAAGAA GATGGTCCAG AATTTTCCAG CTCCTCTCTG 
GACTCTCACA TGTGCAAAAA CTACCGAATA TTACCCAGGG TGGGATATCT TTGCCCAAAG 
AATCTAAACC CTGTCTGTGG TGATGATGGC CAAACATATG CCAACCCTTG TATGCTCTGT 
CATGAAAACT TGATGCGTCA GACTAATACA CACATACATA AACAAGGAAC ATGTGAGGAG 
AACAGTAACC TGGAAGCAGT GTCTGCTGGC ACACTTGCCT CTGAAAAAAT GATGCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001164077.1
CDS	66..3125
Translation	MKTATVPMLLTLAFYLTQDASSWKGNQDPCMKFRALMKNGTLSCPQGNNSSQSLNDIIFQSECILCKKTLEQETNTKDMVMVRFLSRATRATAPAKLNCENFKQRRKDGDFICPSDASAVCGTDGKTYPSRCHLCAENAKSQSHVEVKSEGECGSSHLETDMCSDFRAYVQDGRLGCTRENDPIRGPDGRTHGNRCAMCAELFLKEAQVNATRDRESRIRRDAEEELCKEFENQVRSGRLFCTRESDPIRGPDGKMHGNKCALCAEIFMKRFTEGKDKAETKQRDAEERAKAKMEIRKRCSEFQDRAKNGTLFCTRENDPIRGLDGKTHGNLCSMCQAFFKTEAEEKKAEAGTRNRRGSEKSETYAKLCDEFRKVRKNGQLYCTRENDPVRGPDGKIHGNICSMCQSFFIQEDKARAKVKREAAKEMCSEFQDQAKNGMLICTRENDPVVGPDGKTHANKCAMCASVFLLEEEEKKNDDKGEREKAEAGKTKREAVQNLCSKYRTLLKNGRLPCTKKNDPIEGLDGKIYASTCSMCEAFFHQEAKKNEAGFGAKAKREVKVDCSEFLTLLRGGELFCTRENDPVRGPDGKTHGNKCAMCKAVFEKENEERKRKEGENQRIAAGQSSGGGNQKAKDECAQYWESMKNGELSCTRESDPVRGADGKPYNNKCVMCKELLRREMEEKNKNSASRSNGTETRKDVCDQFRNQMKNGKLLCTRESDPTRGPDGKTHGNKCAMCKERLEREAAEKKKKEDEDKRNTGANKSNKKDQCHEFRSMQLNGRLICTRENDPIRGPDGKTHVNKCAMCQMMFEHEASERKMRHEENSRSQSTNEAEDNCGEVHNTAQDVKPRQARSSLPSIRGISEDECSNFQNLISNGKLICPEMDDPLHGADGSFYQNKCNMCRDVLEKKALERSGFQEKSSQIRSTKEDGPEFSSSSLDSHMCKNYRILPRVGYLCPKNLNPVCGDDGQTYANPCMLCHENLMRQTNTHIHKQGTCEENSNLEAVSAGTLASEKMMQ

Target ORF information:

RefSeq Version	NM_001170606.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus serine peptidase inhibitor, Kazal type 5 (Spink5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170606.1

ORF Insert Sequence:

ATGAAGACAG CCACGGTGCC AATGCTTCTC ACCCTGGCAT TTTATCTCAC ACAAGATGCC 
TCCAGTTGGA AAGGAAATCA GGACCCATGC ATGAAATTTC GAGCACTGAT GAAAAATGGG 
ACACTGTCAT GTCCACAAGG CAACAATTCT TCTCAAAGTC TCAATGACAT TATTTTCCAA 
AGTGAATGCA TCTTGTGCAA AAAAACACTA GAACAAGAAA CAAATACCAA GGACATGGTG 
ATGGTGAGGT TTTTGTCAAG AGCCACCAGG GCCACTGCCC CAGCAAAGTT GAATTGTGAG 
AACTTCAAGC AAAGAAGAAA AGATGGGGAT TTCATCTGTC CATCTGATGC TTCTGCAGTT 
TGTGGCACAG ATGGGAAAAC GTACCCCAGC AGATGTCACC TGTGTGCTGA GAATGCGAAA 
AGCCAGAGCC ATGTTGAAGT AAAAAGTGAA GGTGAATGTG GCAGCAGCCA CCTAGAGACG 
GATATGTGCA GTGATTTTCG GGCCTACGTG CAAGATGGAA GACTCGGATG TACAAGGGAA 
AATGATCCAA TCCGTGGTCC TGATGGGAGG ACACATGGCA ATAGGTGTGC CATGTGTGCT 
GAGCTCTTTT TAAAAGAAGC TCAAGTAAAT GCCACACGTG ACAGAGAAAG CAGAATTCGG 
AGAGATGCCG AGGAGGAACT TTGCAAGGAA TTTGAAAACC AAGTGAGGAG TGGAAGACTC 
TTTTGTACCC GAGAAAGTGA CCCAATCCGT GGCCCTGATG GCAAGATGCA TGGGAACAAG 
TGTGCCCTCT GTGCTGAGAT ATTCATGAAG CGTTTTACAG AAGGAAAAGA TAAAGCAGAG 
ACAAAACAGA GAGATGCTGA AGAAAGGGCT AAAGCTAAAA TGGAAATTAG GAAACGATGC 
AGTGAATTCC AGGATCGTGC AAAGAATGGA ACACTGTTCT GTACCAGAGA AAATGATCCT 
ATTCGTGGTC TAGATGGAAA AACACATGGC AACTTGTGTT CCATGTGCCA AGCTTTCTTC 
AAAACAGAAG CTGAAGAAAA GAAGGCTGAA GCTGGAACCA GAAACAGAAG AGGGTCTGAA 
AAATCAGAAA CATATGCAAA GCTATGTGAT GAATTTCGGA AGGTCAGAAA GAATGGACAA 
CTATATTGCA CCAGAGAGAA TGACCCAGTC AGGGGTCCTG ATGGGAAAAT CCATGGGAAC 
ATATGCTCCA TGTGTCAGTC TTTCTTTATT CAAGAAGACA AAGCAAGAGC AAAGGTTAAA 
AGAGAAGCTG CAAAGGAAAT GTGCAGTGAG TTCCAGGACC AAGCAAAGAA TGGAATGCTC 
ATTTGCACCA GAGAAAATGA CCCTGTTGTT GGCCCAGATG GAAAAACACA TGCAAACAAG 
TGTGCAATGT GTGCAAGTGT GTTTCTACTA GAAGAAGAAG AGAAGAAAAA TGATGACAAA 
GGGGAAAGGG AGAAGGCTGA GGCTGGAAAG ACGAAGAGAG AAGCAGTGCA GAATCTATGT 
AGTAAATATC GTACCTTGCT AAAAAATGGG CGGCTCCCCT GCACCAAAAA GAATGACCCC 
ATTGAGGGCC TGGATGGGAA GATCTATGCA AGTACCTGCT CCATGTGTGA GGCCTTCTTC 
CACCAAGAAG CGAAGAAAAA TGAAGCTGGC TTCGGAGCAA AGGCTAAAAG AGAAGTTAAG 
GTTGATTGCA GTGAATTTCT GACTCTTTTA AGAGGTGGAG AACTCTTTTG CACACGAGAA 
AATGACCCTG TGCGTGGCCC AGATGGCAAG ACCCATGGCA ACAAGTGTGC CATGTGCAAG 
GCTGTCTTTG AGAAAGAAAA TGAAGAAAGG AAGAGGAAAG AAGGGGAAAA CCAGAGAATT 
GCTGCAGGGC AGAGTTCCGG TGGTGGGAAC CAGAAAGCTA AGGATGAATG TGCTCAATAT 
TGGGAAAGCA TGAAAAATGG AGAACTTAGC TGTACTCGGG AGAGTGACCC TGTACGTGGT 
GCTGATGGGA AACCATACAA CAATAAATGT GTCATGTGTA AAGAACTACT GCGGAGAGAA 
ATGGAGGAAA AAAATAAAAA TTCTGCCTCA AGGTCAAATG GGACTGAAAC AAGAAAGGAT 
GTATGCGACC AGTTTAGAAA CCAAATGAAA AATGGGAAAC TCCTGTGCAC TCGGGAAAGC 
GACCCTACCC GGGGTCCAGA TGGCAAGACC CATGGCAATA AGTGTGCCAT GTGCAAGGAG 
AGATTGGAAA GAGAAGCTGC TGAAAAGAAA AAGAAAGAGG ATGAAGACAA GAGAAACACA 
GGAGCAAACA AGAGCAATAA GAAGGATCAG TGTCATGAAT TCCGGAGCAT GCAGCTAAAT 
GGAAGGCTGA TCTGCACCCG AGAAAATGAT CCCATTCGAG GTCCAGATGG CAAGACACAT 
GTAAACAAGT GTGCCATGTG TCAGATGATG TTTGAGCATG AAGCCAGTGA AAGAAAAATG 
AGACATGAAG AAAACTCACG CTCCCAGTCC ACAAATGAGG CAGAGGACAA CTGTGGAGAA 
GTTCATAACA CAGCACAGGA TGTCAAACCC AGGCAGGCCA GGAGTTCTTT GCCCTCCATA 
CGTGGGATCA GTGAAGATGA GTGCAGCAAT TTTCAAAATC TCATAAGTAA TGGAAAGCTT 
ATCTGTCCAG AAATGGATGA TCCATTGCAT GGTGCTGATG GAAGCTTTTA TCAGAACAAG 
TGCAACATGT GCAGAGATGT CCTTGAAAAA AAAGCTTTAG AAAGGTCAGG ATTTCAAGAA 
AAGTCATCAC AAATTAGATC TACTAAAGAA GATGGTCCAG AATTTTCCAG CTCCTCTCTG 
GACTCTCACA TGTGCAAAAA CTACCGAATA TTACCCAGGG TGGGATATCT TTGCCCAAAG 
AATCTAAACC CTGTCTGTGG TGATGATGGC CAAACATATG CCAACCCTTG TATGCTCTGT 
CATGAAAACT TGATGCGTCA GACTAATACA CACATACATA AACAAGGAAC ATGTGAGGAG 
AACAGTAACC TGGAAGCAGT GTCTGCTGGC ACACTTGCCT CTGAAAAAAT GATGCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spink5 ( XM_017600996.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017456485.1 Rattus norvegicus serine peptidase inhibitor, Kazal type 5 (Spink5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa06383
Clone ID Related Accession (Same CDS sequence)	NM_001170606.1 , XM_017600996.1
Accession Version	XM_017600996.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3060bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	serine peptidase inhibitor, Kazal type 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005117.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGACAG CCACGGTGCC AATGCTTCTC ACCCTGGCAT TTTATCTCAC ACAAGATGCC 
TCCAGTTGGA AAGGAAATCA GGACCCATGC ATGAAATTTC GAGCACTGAT GAAAAATGGG 
ACACTGTCAT GTCCACAAGG CAACAATTCT TCTCAAAGTC TCAATGACAT TATTTTCCAA 
AGTGAATGCA TCTTGTGCAA AAAAACACTA GAACAAGAAA CAAATACCAA GGACATGGTG 
ATGGTGAGGT TTTTGTCAAG AGCCACCAGG GCCACTGCCC CAGCAAAGTT GAATTGTGAG 
AACTTCAAGC AAAGAAGAAA AGATGGGGAT TTCATCTGTC CATCTGATGC TTCTGCAGTT 
TGTGGCACAG ATGGGAAAAC GTACCCCAGC AGATGTCACC TGTGTGCTGA GAATGCGAAA 
AGCCAGAGCC ATGTTGAAGT AAAAAGTGAA GGTGAATGTG GCAGCAGCCA CCTAGAGACG 
GATATGTGCA GTGATTTTCG GGCCTACGTG CAAGATGGAA GACTCGGATG TACAAGGGAA 
AATGATCCAA TCCGTGGTCC TGATGGGAGG ACACATGGCA ATAGGTGTGC CATGTGTGCT 
GAGCTCTTTT TAAAAGAAGC TCAAGTAAAT GCCACACGTG ACAGAGAAAG CAGAATTCGG 
AGAGATGCCG AGGAGGAACT TTGCAAGGAA TTTGAAAACC AAGTGAGGAG TGGAAGACTC 
TTTTGTACCC GAGAAAGTGA CCCAATCCGT GGCCCTGATG GCAAGATGCA TGGGAACAAG 
TGTGCCCTCT GTGCTGAGAT ATTCATGAAG CGTTTTACAG AAGGAAAAGA TAAAGCAGAG 
ACAAAACAGA GAGATGCTGA AGAAAGGGCT AAAGCTAAAA TGGAAATTAG GAAACGATGC 
AGTGAATTCC AGGATCGTGC AAAGAATGGA ACACTGTTCT GTACCAGAGA AAATGATCCT 
ATTCGTGGTC TAGATGGAAA AACACATGGC AACTTGTGTT CCATGTGCCA AGCTTTCTTC 
AAAACAGAAG CTGAAGAAAA GAAGGCTGAA GCTGGAACCA GAAACAGAAG AGGGTCTGAA 
AAATCAGAAA CATATGCAAA GCTATGTGAT GAATTTCGGA AGGTCAGAAA GAATGGACAA 
CTATATTGCA CCAGAGAGAA TGACCCAGTC AGGGGTCCTG ATGGGAAAAT CCATGGGAAC 
ATATGCTCCA TGTGTCAGTC TTTCTTTATT CAAGAAGACA AAGCAAGAGC AAAGGTTAAA 
AGAGAAGCTG CAAAGGAAAT GTGCAGTGAG TTCCAGGACC AAGCAAAGAA TGGAATGCTC 
ATTTGCACCA GAGAAAATGA CCCTGTTGTT GGCCCAGATG GAAAAACACA TGCAAACAAG 
TGTGCAATGT GTGCAAGTGT GTTTCTACTA GAAGAAGAAG AGAAGAAAAA TGATGACAAA 
GGGGAAAGGG AGAAGGCTGA GGCTGGAAAG ACGAAGAGAG AAGCAGTGCA GAATCTATGT 
AGTAAATATC GTACCTTGCT AAAAAATGGG CGGCTCCCCT GCACCAAAAA GAATGACCCC 
ATTGAGGGCC TGGATGGGAA GATCTATGCA AGTACCTGCT CCATGTGTGA GGCCTTCTTC 
CACCAAGAAG CGAAGAAAAA TGAAGCTGGC TTCGGAGCAA AGGCTAAAAG AGAAGTTAAG 
GTTGATTGCA GTGAATTTCT GACTCTTTTA AGAGGTGGAG AACTCTTTTG CACACGAGAA 
AATGACCCTG TGCGTGGCCC AGATGGCAAG ACCCATGGCA ACAAGTGTGC CATGTGCAAG 
GCTGTCTTTG AGAAAGAAAA TGAAGAAAGG AAGAGGAAAG AAGGGGAAAA CCAGAGAATT 
GCTGCAGGGC AGAGTTCCGG TGGTGGGAAC CAGAAAGCTA AGGATGAATG TGCTCAATAT 
TGGGAAAGCA TGAAAAATGG AGAACTTAGC TGTACTCGGG AGAGTGACCC TGTACGTGGT 
GCTGATGGGA AACCATACAA CAATAAATGT GTCATGTGTA AAGAACTACT GCGGAGAGAA 
ATGGAGGAAA AAAATAAAAA TTCTGCCTCA AGGTCAAATG GGACTGAAAC AAGAAAGGAT 
GTATGCGACC AGTTTAGAAA CCAAATGAAA AATGGGAAAC TCCTGTGCAC TCGGGAAAGC 
GACCCTACCC GGGGTCCAGA TGGCAAGACC CATGGCAATA AGTGTGCCAT GTGCAAGGAG 
AGATTGGAAA GAGAAGCTGC TGAAAAGAAA AAGAAAGAGG ATGAAGACAA GAGAAACACA 
GGAGCAAACA AGAGCAATAA GAAGGATCAG TGTCATGAAT TCCGGAGCAT GCAGCTAAAT 
GGAAGGCTGA TCTGCACCCG AGAAAATGAT CCCATTCGAG GTCCAGATGG CAAGACACAT 
GTAAACAAGT GTGCCATGTG TCAGATGATG TTTGAGCATG AAGCCAGTGA AAGAAAAATG 
AGACATGAAG AAAACTCACG CTCCCAGTCC ACAAATGAGG CAGAGGACAA CTGTGGAGAA 
GTTCATAACA CAGCACAGGA TGTCAAACCC AGGCAGGCCA GGAGTTCTTT GCCCTCCATA 
CGTGGGATCA GTGAAGATGA GTGCAGCAAT TTTCAAAATC TCATAAGTAA TGGAAAGCTT 
ATCTGTCCAG AAATGGATGA TCCATTGCAT GGTGCTGATG GAAGCTTTTA TCAGAACAAG 
TGCAACATGT GCAGAGATGT CCTTGAAAAA AAAGCTTTAG AAAGGTCAGG ATTTCAAGAA 
AAGTCATCAC AAATTAGATC TACTAAAGAA GATGGTCCAG AATTTTCCAG CTCCTCTCTG 
GACTCTCACA TGTGCAAAAA CTACCGAATA TTACCCAGGG TGGGATATCT TTGCCCAAAG 
AATCTAAACC CTGTCTGTGG TGATGATGGC CAAACATATG CCAACCCTTG TATGCTCTGT 
CATGAAAACT TGATGCGTCA GACTAATACA CACATACATA AACAAGGAAC ATGTGAGGAG 
AACAGTAACC TGGAAGCAGT GTCTGCTGGC ACACTTGCCT CTGAAAAAAT GATGCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017456485.1
CDS	134..3193
Translation	MKTATVPMLLTLAFYLTQDASSWKGNQDPCMKFRALMKNGTLSCPQGNNSSQSLNDIIFQSECILCKKTLEQETNTKDMVMVRFLSRATRATAPAKLNCENFKQRRKDGDFICPSDASAVCGTDGKTYPSRCHLCAENAKSQSHVEVKSEGECGSSHLETDMCSDFRAYVQDGRLGCTRENDPIRGPDGRTHGNRCAMCAELFLKEAQVNATRDRESRIRRDAEEELCKEFENQVRSGRLFCTRESDPIRGPDGKMHGNKCALCAEIFMKRFTEGKDKAETKQRDAEERAKAKMEIRKRCSEFQDRAKNGTLFCTRENDPIRGLDGKTHGNLCSMCQAFFKTEAEEKKAEAGTRNRRGSEKSETYAKLCDEFRKVRKNGQLYCTRENDPVRGPDGKIHGNICSMCQSFFIQEDKARAKVKREAAKEMCSEFQDQAKNGMLICTRENDPVVGPDGKTHANKCAMCASVFLLEEEEKKNDDKGEREKAEAGKTKREAVQNLCSKYRTLLKNGRLPCTKKNDPIEGLDGKIYASTCSMCEAFFHQEAKKNEAGFGAKAKREVKVDCSEFLTLLRGGELFCTRENDPVRGPDGKTHGNKCAMCKAVFEKENEERKRKEGENQRIAAGQSSGGGNQKAKDECAQYWESMKNGELSCTRESDPVRGADGKPYNNKCVMCKELLRREMEEKNKNSASRSNGTETRKDVCDQFRNQMKNGKLLCTRESDPTRGPDGKTHGNKCAMCKERLEREAAEKKKKEDEDKRNTGANKSNKKDQCHEFRSMQLNGRLICTRENDPIRGPDGKTHVNKCAMCQMMFEHEASERKMRHEENSRSQSTNEAEDNCGEVHNTAQDVKPRQARSSLPSIRGISEDECSNFQNLISNGKLICPEMDDPLHGADGSFYQNKCNMCRDVLEKKALERSGFQEKSSQIRSTKEDGPEFSSSSLDSHMCKNYRILPRVGYLCPKNLNPVCGDDGQTYANPCMLCHENLMRQTNTHIHKQGTCEENSNLEAVSAGTLASEKMMQ

Target ORF information:

RefSeq Version	XM_017600996.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus serine peptidase inhibitor, Kazal type 5 (Spink5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017600996.1

ORF Insert Sequence:

ATGAAGACAG CCACGGTGCC AATGCTTCTC ACCCTGGCAT TTTATCTCAC ACAAGATGCC 
TCCAGTTGGA AAGGAAATCA GGACCCATGC ATGAAATTTC GAGCACTGAT GAAAAATGGG 
ACACTGTCAT GTCCACAAGG CAACAATTCT TCTCAAAGTC TCAATGACAT TATTTTCCAA 
AGTGAATGCA TCTTGTGCAA AAAAACACTA GAACAAGAAA CAAATACCAA GGACATGGTG 
ATGGTGAGGT TTTTGTCAAG AGCCACCAGG GCCACTGCCC CAGCAAAGTT GAATTGTGAG 
AACTTCAAGC AAAGAAGAAA AGATGGGGAT TTCATCTGTC CATCTGATGC TTCTGCAGTT 
TGTGGCACAG ATGGGAAAAC GTACCCCAGC AGATGTCACC TGTGTGCTGA GAATGCGAAA 
AGCCAGAGCC ATGTTGAAGT AAAAAGTGAA GGTGAATGTG GCAGCAGCCA CCTAGAGACG 
GATATGTGCA GTGATTTTCG GGCCTACGTG CAAGATGGAA GACTCGGATG TACAAGGGAA 
AATGATCCAA TCCGTGGTCC TGATGGGAGG ACACATGGCA ATAGGTGTGC CATGTGTGCT 
GAGCTCTTTT TAAAAGAAGC TCAAGTAAAT GCCACACGTG ACAGAGAAAG CAGAATTCGG 
AGAGATGCCG AGGAGGAACT TTGCAAGGAA TTTGAAAACC AAGTGAGGAG TGGAAGACTC 
TTTTGTACCC GAGAAAGTGA CCCAATCCGT GGCCCTGATG GCAAGATGCA TGGGAACAAG 
TGTGCCCTCT GTGCTGAGAT ATTCATGAAG CGTTTTACAG AAGGAAAAGA TAAAGCAGAG 
ACAAAACAGA GAGATGCTGA AGAAAGGGCT AAAGCTAAAA TGGAAATTAG GAAACGATGC 
AGTGAATTCC AGGATCGTGC AAAGAATGGA ACACTGTTCT GTACCAGAGA AAATGATCCT 
ATTCGTGGTC TAGATGGAAA AACACATGGC AACTTGTGTT CCATGTGCCA AGCTTTCTTC 
AAAACAGAAG CTGAAGAAAA GAAGGCTGAA GCTGGAACCA GAAACAGAAG AGGGTCTGAA 
AAATCAGAAA CATATGCAAA GCTATGTGAT GAATTTCGGA AGGTCAGAAA GAATGGACAA 
CTATATTGCA CCAGAGAGAA TGACCCAGTC AGGGGTCCTG ATGGGAAAAT CCATGGGAAC 
ATATGCTCCA TGTGTCAGTC TTTCTTTATT CAAGAAGACA AAGCAAGAGC AAAGGTTAAA 
AGAGAAGCTG CAAAGGAAAT GTGCAGTGAG TTCCAGGACC AAGCAAAGAA TGGAATGCTC 
ATTTGCACCA GAGAAAATGA CCCTGTTGTT GGCCCAGATG GAAAAACACA TGCAAACAAG 
TGTGCAATGT GTGCAAGTGT GTTTCTACTA GAAGAAGAAG AGAAGAAAAA TGATGACAAA 
GGGGAAAGGG AGAAGGCTGA GGCTGGAAAG ACGAAGAGAG AAGCAGTGCA GAATCTATGT 
AGTAAATATC GTACCTTGCT AAAAAATGGG CGGCTCCCCT GCACCAAAAA GAATGACCCC 
ATTGAGGGCC TGGATGGGAA GATCTATGCA AGTACCTGCT CCATGTGTGA GGCCTTCTTC 
CACCAAGAAG CGAAGAAAAA TGAAGCTGGC TTCGGAGCAA AGGCTAAAAG AGAAGTTAAG 
GTTGATTGCA GTGAATTTCT GACTCTTTTA AGAGGTGGAG AACTCTTTTG CACACGAGAA 
AATGACCCTG TGCGTGGCCC AGATGGCAAG ACCCATGGCA ACAAGTGTGC CATGTGCAAG 
GCTGTCTTTG AGAAAGAAAA TGAAGAAAGG AAGAGGAAAG AAGGGGAAAA CCAGAGAATT 
GCTGCAGGGC AGAGTTCCGG TGGTGGGAAC CAGAAAGCTA AGGATGAATG TGCTCAATAT 
TGGGAAAGCA TGAAAAATGG AGAACTTAGC TGTACTCGGG AGAGTGACCC TGTACGTGGT 
GCTGATGGGA AACCATACAA CAATAAATGT GTCATGTGTA AAGAACTACT GCGGAGAGAA 
ATGGAGGAAA AAAATAAAAA TTCTGCCTCA AGGTCAAATG GGACTGAAAC AAGAAAGGAT 
GTATGCGACC AGTTTAGAAA CCAAATGAAA AATGGGAAAC TCCTGTGCAC TCGGGAAAGC 
GACCCTACCC GGGGTCCAGA TGGCAAGACC CATGGCAATA AGTGTGCCAT GTGCAAGGAG 
AGATTGGAAA GAGAAGCTGC TGAAAAGAAA AAGAAAGAGG ATGAAGACAA GAGAAACACA 
GGAGCAAACA AGAGCAATAA GAAGGATCAG TGTCATGAAT TCCGGAGCAT GCAGCTAAAT 
GGAAGGCTGA TCTGCACCCG AGAAAATGAT CCCATTCGAG GTCCAGATGG CAAGACACAT 
GTAAACAAGT GTGCCATGTG TCAGATGATG TTTGAGCATG AAGCCAGTGA AAGAAAAATG 
AGACATGAAG AAAACTCACG CTCCCAGTCC ACAAATGAGG CAGAGGACAA CTGTGGAGAA 
GTTCATAACA CAGCACAGGA TGTCAAACCC AGGCAGGCCA GGAGTTCTTT GCCCTCCATA 
CGTGGGATCA GTGAAGATGA GTGCAGCAAT TTTCAAAATC TCATAAGTAA TGGAAAGCTT 
ATCTGTCCAG AAATGGATGA TCCATTGCAT GGTGCTGATG GAAGCTTTTA TCAGAACAAG 
TGCAACATGT GCAGAGATGT CCTTGAAAAA AAAGCTTTAG AAAGGTCAGG ATTTCAAGAA 
AAGTCATCAC AAATTAGATC TACTAAAGAA GATGGTCCAG AATTTTCCAG CTCCTCTCTG 
GACTCTCACA TGTGCAAAAA CTACCGAATA TTACCCAGGG TGGGATATCT TTGCCCAAAG 
AATCTAAACC CTGTCTGTGG TGATGATGGC CAAACATATG CCAACCCTTG TATGCTCTGT 
CATGAAAACT TGATGCGTCA GACTAATACA CACATACATA AACAAGGAAC ATGTGAGGAG 
AACAGTAACC TGGAAGCAGT GTCTGCTGGC ACACTTGCCT CTGAAAAAAT GATGCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Elastase 2 is expressed in human and mouse epidermis and impairs skin barrier function in Netherton syndrome through filaggrin and lipid misprocessing.
The Journal of clinical investigation120(3)871-82(2010 Mar)
Bonnart C,Deraison C,Lacroix M,Uchida Y,Besson C,Robin A,Briot A,Gonthier M,Lamant L,Dubus P,Monsarrat B,Hovnanian A

Kallikrein-mediated proteolysis regulates the antimicrobial effects of cathelicidins in skin.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology20(12)2068-80(2006 Oct)
Yamasaki K,Schauber J,Coda A,Lin H,Dorschner RA,Schechter NM,Bonnart C,Descargues P,Hovnanian A,Gallo RL

Consequences of C-terminal domains and N-terminal signal peptide deletions on LEKTI secretion, stability, and subcellular distribution.
Archives of biochemistry and biophysics435(1)89-102(2005 Mar)
Jayakumar A,Kang Y,Henderson Y,Mitsudo K,Liu X,Briggs K,Wang M,Frederick MJ,El-Naggar AK,Bebök Z,Clayman GL

LEKTI is localized in lamellar granules, separated from KLK5 and KLK7, and is secreted in the extracellular spaces of the superficial stratum granulosum.
The Journal of investigative dermatology124(2)360-6(2005 Feb)
Ishida-Yamamoto A,Deraison C,Bonnart C,Bitoun E,Robinson R,O'Brien TJ,Wakamatsu K,Ohtsubo S,Takahashi H,Hashimoto Y,Dopping-Hepenstal PJ,McGrath JA,Iizuka H,Richard G,Hovnanian A

Epidermal detachment, desmosomal dissociation, and destabilization of corneodesmosin in Spink5-/- mice.
Genes & development18(19)2354-8(2004 Oct)
Yang T,Liang D,Koch PJ,Hohl D,Kheradmand F,Overbeek PA

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Spink5 cDNA ORF clone, Rattus norvegicus(Norway rat)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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