Man2b2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Man2b2
Entrez Gene ID	360955
Full Name	mannosidase, alpha, class 2B, member 2
Synonyms	Ab2-450
General protein information	Preferred Names mannosidase, alpha, class 2B, member 2 Names epididymis-specific alpha-mannosidase mannosidase 2, alpha B2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 14 Map Location: 14q21

mRNA and Protein(s)

mRNA	Protein	Name
NM_001134971.2	NP_001128443.1	epididymis-specific alpha-mannosidase precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MAN2B2	XP_003310289.1
Macaca mulatta (Rhesus monkey)	MAN2B2	XP_002804053.1
Bos taurus (cattle)	LOC532207	XP_003586284.2
Gallus gallus (chicken)	MAN2B2	XP_420805.2
Bos taurus (cattle)	MAN2B2	XP_002688458.1
Mus musculus (house mouse)	Man2b2	NP_032576.2
Canis lupus familiaris (dog)	LOC100855835	XP_005618789.1
Canis lupus familiaris (dog)	MAN2B2	XP_005642316.1
Rattus norvegicus (Norway rat)	Man2b2	NP_001128443.1
Homo sapiens (human)	MAN2B2	NP_056089.1
Danio rerio (zebrafish)	man2b2	XP_001337519.3
Xenopus tropicalis (tropical clawed frog)	man2b2	NP_001106515.1

Gene Ontology
Bibliography

Related articles in PubMed

Extracellular matrix secretion by cardiac fibroblasts: role of microRNA-29b and microRNA-30c.
Abonnenc M, Nabeebaccus AA, Mayr U, Barallobre-Barreiro J, Dong X, Cuello F, Sur S, Drozdov I, Langley SR, Lu R, Stathopoulou K, Didangelos A, Yin X, Zimmermann WH, Shah AM, Zampetaki A, Mayr M
Circulation research113(10)1138-47(2013 Oct)

Gene and alternative splicing annotation with AIR.
Florea L, Di Francesco V, Miller J, Turner R, Yao A, Harris M, Walenz B, Mobarry C, Merkulov GV, Charlab R, Dew I, Deng Z, Istrail S, Li P, Sutton G
Genome research15(1)54-66(2005 Jan)

The core-specific lysosomal alpha(1-6)-mannosidase activity depends on aspartamidohydrolase activity.
Haeuw JF, Grard T, Alonso C, Strecker G, Michalski JC
The Biochemical journal297 ( Pt 3)463-6(1994 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Man2b2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Man2b2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa03772	NM_001134971.2 Latest version!	Rattus norvegicus mannosidase, alpha, class 2B, member 2 (Man2b2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Man2b2 ( NM_001134971.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001128443.1 Rattus norvegicus mannosidase, alpha, class 2B, member 2 (Man2b2), mRNA.

Price & Availability↑

CloneID	ORa03772
Clone ID Related Accession (Same CDS sequence)	NM_001134971.2
Accession Version	NM_001134971.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3015bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1527350400000
Organism	Rattus norvegicus(Norway rat)
Product	epididymis-specific alpha-mannosidase precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC168692.1. On Aug 30, 2012 this sequence version replaced NM_001134971.1. ##Evidence-Data-START## Transcript exon combination :: BC168692.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGGGCCGC TGCGCTGGCT GCCGCTGCTC GGCCAGCTAT TGCTGCTGTG GCCGCTGGCG 
GCCCGGCCCG CCAGCCCCAT CCGGGCCTTT GTGGTGCCCC ACAGTCACAT GGACGTGGGC 
TGGGTCTACA CGGTCCAGGA AAGCATGCGG GCCTATGCAG CCAATGTCTA CACCACCGTG 
GTGGAGGAAC TGGTCCGAGG CAGGCAGCGC CGCTTCATCG CTGTGGAGCA GGAGTTCTTC 
CGGCTGTGGT GGGATGGTGT AGCCTCTGAG CAGCAGAAAC AGCAGGTCCG CCAGCTCCTG 
GATGAAGGAT GCCTGGAGTT CGTTCTTGGA GGCCAGGTCA TGCATGACGA GGCTGTGACC 
CACCTGGATG ACCAGATCCT ACAGCTCACA GAAGGACATG GCTTTCTCTA CGAAACGTTT 
GGAATCCGGC CACAGTTCTC CTGGCACGTG GACCCCTTTG GTGCATCAGC GACGACCCCT 
ACCCTATTTT CCCTGGCCGG CTTCAATGCC CACCTCATCT CCCGGATTGA CTACGACCTC 
AAGGATGCCA TGCAGGAAGC TCAGGGGCTA CAGTTCGTGT GGCACGGGTC CCCATCTCTG 
TCAGAGCAGC AGGAGATCTA CACCCACGTC ATGGACCTTT ATAGCTACTG CACCCCCTCA 
CACATCCCTT TCTCTAACAG GTCTGGATTT TACTGGAATG GTGTGGCCGT CTTCCCAGAG 
CCACCCCCAG ATGGCGTGTA TCCTAACATG AGTGAACCTG TCACCGTAGA CAACATCCAC 
CTCTACGCTG AGGCTCTGGT GGCCAACATT AAACAGAGGG CCGCATGGTT CCGGACTCCC 
CACGTCCTCT GGCCCTGGGG ATGTGACAAG CAGTTCTTCA ACGCCTCGGT GCAGTTTGAC 
AACATGGACC CCCTGCTGGA GTACATTAAC AACCGCTCTG CCCAGTTTGG CATCTCAGTG 
CAGTACGCCA CACTGAATGA TTACTTCCAG GCCCTGCACG ACTTCAACGT GACCTGGGAC 
ATCCGAGACC ACCATGACTT CCTACCCTAT TCTTCAGAGC CACAGCAGGC CTGGACCGGA 
TTCTACACAT CCCGGAGCAC TCTCAAGGGT CTAGCGAGAC AAGCCAGTGC CTTGCTATAT 
GCTGGGGAGT CCATGTTCAC ACGCTATATG TGGCCACACC CCCGTGGAAC TCTGGACCCT 
ACCTGGGCCC TGCAGCAGCT CCAACAGCTT CGCTGGGCCG TGTCTGAGGT CCAGCACCAC 
GATGCCATCA CAGGCACTGA ATCCCCCAAG GTGAAAAACA TGTACATGGA ACATCTCAGC 
ATGGGGATGC GAGGTGTACA TAAGCTGATG GCCTCCATTG CCCTGGACAG GTCCCCAGGG 
TCTGGCACAG ACACGAGGCC TGTGGGATAC TCTGCCTCTG TGTACAACCC GCTAGCCTGG 
AAGATCACTA CCATTATCAC CCTGACTGTC GCTTTCCCCA GTGTCAGCGT CACAGATGAG 
TTGGGCCGTC CAGTGTCAGC ACAGATTCAG AACTCGACAA AGGACCCTTC TGCGTATGAC 
CTGCTCATCC TGACCACCAT CCCAGGCCTC AGCTACCGGT ACTACAAAGT CAGGCGCATA 
CTCGGGGACC AGGCAGGCAC CAGAGAGCTG GTCACCCCCA GGGCCAACAC CCTCAAGTTT 
AGTCGCAAAC TGAGGAACCA GACCAGCCAG GAAGGCAAGC TCCTGGTGCC TGTAATGAAT 
GACTGCTACA CTTTGCTGTT CGACCAGGAC ACCAACATGT TACATAGCAT TCAGGACAGA 
CAGAGTAACC GCACAGTGCA CATGACCCAA GAGTTCCTGG AGTATCGAGC CAACGGGGAC 
GTGAAGCAGG GCCCCATTTC TGACAACTAC CTCTTTGCAT CCAACGATAC CGCTGAGCCT 
TCCTGGGAAG CGGTGGGGAT GGAGATGGTG GTGGGCACGC TGGCCACGGA CGTAAGGCAG 
TATTTCTACA GGAACATAAC AGACCAGGGT TACATCTATT CGATCCACAC TCGTCTGGCC 
CATGTGCATC CGAGCCTTGC TGGGGAACTG CTCTGCCGAC GGATAGAGCA GCAGTACCAA 
GTGGGCCCCC TGGACCTAAA CCGTGAGGCC ATCCTGAGGA CCAGCAGTGA CCTAAACAGC 
CAACAAGTCC TCTACTCTGA CAACAATGGC TACCAGATGC AGCGGAGGCC CTACAAGGCT 
TTCAAGAGCA ATCCCATCCC CCGGAATTAC TACCCCATGG TACAGTCGGC CTTCATCGAG 
GATGACAAGA GCAGGCTGGT GCTGCTGGCA GAGCGACCGC ATGGTGTCTC CAGCCAAGAG 
AATGGGCAGG TGGAGGTAAT GCTCCATCGG CGGCTGTGGA ACAACCTGGT CTGGGACCTG 
AACTACAACC TCACACTGAA TGACACCTCT GTCGTCCACC CAGTGCTCTG GCTTCTGCTG 
GGGCCCAAGT CCACCACAAC TGCCCTGCGC CCGAGGAGTG GGGTGGCCCT CCAGCACAGG 
CCTGTCGTGT TGTTAAAAGA GCTAACTGAT GAAGAAGAGA CAGCCATTCA CAGACCCCAT 
CACCCCTGGC CTGTGACATT GCCCCCAAGC CTGCACCTGC AGGTCCTCAG CTTACGTGGC 
TGGCAGTACA GCAGGAACCA TGCCCAGCAC CTGAAGAACC TTCAGAGAGG CCACCCAGAG 
AAACCACAGG TCAACCTACA ACGTGTGCTG CTGCGCCTGC ACCACCTGTA TGAAGCAGGG 
GAGGACCCGG TACTGTCTCG GCCTGCTACA GTGGATCTCA AGATTGCACT TCGAGGACTA 
GGGTCTGTAG TGGCCGTGGA GGAACGCTCA CTCACAGGGA CCTGGGATGT GCAGACGCTG 
CAGCGCTGGC ACTGGAGGAC AAAGATTGGC CAGCTAAAAG GGCACCCTAC CTCTCCACCA 
AGGTCACCAG GAGGCTCCAT CATCACTGTG TACCCCAAGG AAATCCGGAC CTTCTTCATT 
TACCTCCAGC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001128443.1
CDS	3..3017
Translation	MGPLRWLPLLGQLLLLWPLAARPASPIRAFVVPHSHMDVGWVYTVQESMRAYAANVYTTVVEELVRGRQRRFIAVEQEFFRLWWDGVASEQQKQQVRQLLDEGCLEFVLGGQVMHDEAVTHLDDQILQLTEGHGFLYETFGIRPQFSWHVDPFGASATTPTLFSLAGFNAHLISRIDYDLKDAMQEAQGLQFVWHGSPSLSEQQEIYTHVMDLYSYCTPSHIPFSNRSGFYWNGVAVFPEPPPDGVYPNMSEPVTVDNIHLYAEALVANIKQRAAWFRTPHVLWPWGCDKQFFNASVQFDNMDPLLEYINNRSAQFGISVQYATLNDYFQALHDFNVTWDIRDHHDFLPYSSEPQQAWTGFYTSRSTLKGLARQASALLYAGESMFTRYMWPHPRGTLDPTWALQQLQQLRWAVSEVQHHDAITGTESPKVKNMYMEHLSMGMRGVHKLMASIALDRSPGSGTDTRPVGYSASVYNPLAWKITTIITLTVAFPSVSVTDELGRPVSAQIQNSTKDPSAYDLLILTTIPGLSYRYYKVRRILGDQAGTRELVTPRANTLKFSRKLRNQTSQEGKLLVPVMNDCYTLLFDQDTNMLHSIQDRQSNRTVHMTQEFLEYRANGDVKQGPISDNYLFASNDTAEPSWEAVGMEMVVGTLATDVRQYFYRNITDQGYIYSIHTRLAHVHPSLAGELLCRRIEQQYQVGPLDLNREAILRTSSDLNSQQVLYSDNNGYQMQRRPYKAFKSNPIPRNYYPMVQSAFIEDDKSRLVLLAERPHGVSSQENGQVEVMLHRRLWNNLVWDLNYNLTLNDTSVVHPVLWLLLGPKSTTTALRPRSGVALQHRPVVLLKELTDEEETAIHRPHHPWPVTLPPSLHLQVLSLRGWQYSRNHAQHLKNLQRGHPEKPQVNLQRVLLRLHHLYEAGEDPVLSRPATVDLKIALRGLGSVVAVEERSLTGTWDVQTLQRWHWRTKIGQLKGHPTSPPRSPGGSIITVYPKEIRTFFIYLQQ

Target ORF information:

RefSeq Version	NM_001134971.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mannosidase, alpha, class 2B, member 2 (Man2b2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001134971.2

ORF Insert Sequence:

ATGGGGCCGC TGCGCTGGCT GCCGCTGCTC GGCCAGCTAT TGCTGCTGTG GCCGCTGGCG 
GCCCGGCCCG CCAGCCCCAT CCGGGCCTTT GTGGTGCCCC ACAGTCACAT GGACGTGGGC 
TGGGTCTACA CGGTCCAGGA AAGCATGCGG GCCTATGCAG CCAATGTCTA CACCACCGTG 
GTGGAGGAAC TGGTCCGAGG CAGGCAGCGC CGCTTCATCG CTGTGGAGCA GGAGTTCTTC 
CGGCTGTGGT GGGATGGTGT AGCCTCTGAG CAGCAGAAAC AGCAGGTCCG CCAGCTCCTG 
GATGAAGGAT GCCTGGAGTT CGTTCTTGGA GGCCAGGTCA TGCATGACGA GGCTGTGACC 
CACCTGGATG ACCAGATCCT ACAGCTCACA GAAGGACATG GCTTTCTCTA CGAAACGTTT 
GGAATCCGGC CACAGTTCTC CTGGCACGTG GACCCCTTTG GTGCATCAGC GACGACCCCT 
ACCCTATTTT CCCTGGCCGG CTTCAATGCC CACCTCATCT CCCGGATTGA CTACGACCTC 
AAGGATGCCA TGCAGGAAGC TCAGGGGCTA CAGTTCGTGT GGCACGGGTC CCCATCTCTG 
TCAGAGCAGC AGGAGATCTA CACCCACGTC ATGGACCTTT ATAGCTACTG CACCCCCTCA 
CACATCCCTT TCTCTAACAG GTCTGGATTT TACTGGAATG GTGTGGCCGT CTTCCCAGAG 
CCACCCCCAG ATGGCGTGTA TCCTAACATG AGTGAACCTG TCACCGTAGA CAACATCCAC 
CTCTACGCTG AGGCTCTGGT GGCCAACATT AAACAGAGGG CCGCATGGTT CCGGACTCCC 
CACGTCCTCT GGCCCTGGGG ATGTGACAAG CAGTTCTTCA ACGCCTCGGT GCAGTTTGAC 
AACATGGACC CCCTGCTGGA GTACATTAAC AACCGCTCTG CCCAGTTTGG CATCTCAGTG 
CAGTACGCCA CACTGAATGA TTACTTCCAG GCCCTGCACG ACTTCAACGT GACCTGGGAC 
ATCCGAGACC ACCATGACTT CCTACCCTAT TCTTCAGAGC CACAGCAGGC CTGGACCGGA 
TTCTACACAT CCCGGAGCAC TCTCAAGGGT CTAGCGAGAC AAGCCAGTGC CTTGCTATAT 
GCTGGGGAGT CCATGTTCAC ACGCTATATG TGGCCACACC CCCGTGGAAC TCTGGACCCT 
ACCTGGGCCC TGCAGCAGCT CCAACAGCTT CGCTGGGCCG TGTCTGAGGT CCAGCACCAC 
GATGCCATCA CAGGCACTGA ATCCCCCAAG GTGAAAAACA TGTACATGGA ACATCTCAGC 
ATGGGGATGC GAGGTGTACA TAAGCTGATG GCCTCCATTG CCCTGGACAG GTCCCCAGGG 
TCTGGCACAG ACACGAGGCC TGTGGGATAC TCTGCCTCTG TGTACAACCC GCTAGCCTGG 
AAGATCACTA CCATTATCAC CCTGACTGTC GCTTTCCCCA GTGTCAGCGT CACAGATGAG 
TTGGGCCGTC CAGTGTCAGC ACAGATTCAG AACTCGACAA AGGACCCTTC TGCGTATGAC 
CTGCTCATCC TGACCACCAT CCCAGGCCTC AGCTACCGGT ACTACAAAGT CAGGCGCATA 
CTCGGGGACC AGGCAGGCAC CAGAGAGCTG GTCACCCCCA GGGCCAACAC CCTCAAGTTT 
AGTCGCAAAC TGAGGAACCA GACCAGCCAG GAAGGCAAGC TCCTGGTGCC TGTAATGAAT 
GACTGCTACA CTTTGCTGTT CGACCAGGAC ACCAACATGT TACATAGCAT TCAGGACAGA 
CAGAGTAACC GCACAGTGCA CATGACCCAA GAGTTCCTGG AGTATCGAGC CAACGGGGAC 
GTGAAGCAGG GCCCCATTTC TGACAACTAC CTCTTTGCAT CCAACGATAC CGCTGAGCCT 
TCCTGGGAAG CGGTGGGGAT GGAGATGGTG GTGGGCACGC TGGCCACGGA CGTAAGGCAG 
TATTTCTACA GGAACATAAC AGACCAGGGT TACATCTATT CGATCCACAC TCGTCTGGCC 
CATGTGCATC CGAGCCTTGC TGGGGAACTG CTCTGCCGAC GGATAGAGCA GCAGTACCAA 
GTGGGCCCCC TGGACCTAAA CCGTGAGGCC ATCCTGAGGA CCAGCAGTGA CCTAAACAGC 
CAACAAGTCC TCTACTCTGA CAACAATGGC TACCAGATGC AGCGGAGGCC CTACAAGGCT 
TTCAAGAGCA ATCCCATCCC CCGGAATTAC TACCCCATGG TACAGTCGGC CTTCATCGAG 
GATGACAAGA GCAGGCTGGT GCTGCTGGCA GAGCGACCGC ATGGTGTCTC CAGCCAAGAG 
AATGGGCAGG TGGAGGTAAT GCTCCATCGG CGGCTGTGGA ACAACCTGGT CTGGGACCTG 
AACTACAACC TCACACTGAA TGACACCTCT GTCGTCCACC CAGTGCTCTG GCTTCTGCTG 
GGGCCCAAGT CCACCACAAC TGCCCTGCGC CCGAGGAGTG GGGTGGCCCT CCAGCACAGG 
CCTGTCGTGT TGTTAAAAGA GCTAACTGAT GAAGAAGAGA CAGCCATTCA CAGACCCCAT 
CACCCCTGGC CTGTGACATT GCCCCCAAGC CTGCACCTGC AGGTCCTCAG CTTACGTGGC 
TGGCAGTACA GCAGGAACCA TGCCCAGCAC CTGAAGAACC TTCAGAGAGG CCACCCAGAG 
AAACCACAGG TCAACCTACA ACGTGTGCTG CTGCGCCTGC ACCACCTGTA TGAAGCAGGG 
GAGGACCCGG TACTGTCTCG GCCTGCTACA GTGGATCTCA AGATTGCACT TCGAGGACTA 
GGGTCTGTAG TGGCCGTGGA GGAACGCTCA CTCACAGGGA CCTGGGATGT GCAGACGCTG 
CAGCGCTGGC ACTGGAGGAC AAAGATTGGC CAGCTAAAAG GGCACCCTAC CTCTCCACCA 
AGGTCACCAG GAGGCTCCAT CATCACTGTG TACCCCAAGG AAATCCGGAC CTTCTTCATT 
TACCTCCAGC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Extracellular matrix secretion by cardiac fibroblasts: role of microRNA-29b and microRNA-30c.
Circulation research113(10)1138-47(2013 Oct)
Abonnenc M,Nabeebaccus AA,Mayr U,Barallobre-Barreiro J,Dong X,Cuello F,Sur S,Drozdov I,Langley SR,Lu R,Stathopoulou K,Didangelos A,Yin X,Zimmermann WH,Shah AM,Zampetaki A,Mayr M

Gene and alternative splicing annotation with AIR.
Genome research15(1)54-66(2005 Jan)
Florea L,Di Francesco V,Miller J,Turner R,Yao A,Harris M,Walenz B,Mobarry C,Merkulov GV,Charlab R,Dew I,Deng Z,Istrail S,Li P,Sutton G

The core-specific lysosomal alpha(1-6)-mannosidase activity depends on aspartamidohydrolase activity.
The Biochemical journal297 ( Pt 3)463-6(1994 Feb)
Haeuw JF,Grard T,Alonso C,Strecker G,Michalski JC

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Man2b2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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