Smug1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Smug1
Entrez Gene ID	315344
Full Name	single-strand-selective monofunctional uracil-DNA glycosylase 1
General protein information	Preferred Names single-strand-selective monofunctional uracil-DNA glycosylase 1 Names single-strand selective monofunctional uracil-DNA glycosylase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q36
Summary	involved in the repair of 5-formyluracil and other oxidized and deaminated base lesions [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017594902.1	XP_017450391.1	single-strand selective monofunctional uracil-DNA glycosylase isoform X1
XM_008765749.2	XP_008763971.1	single-strand selective monofunctional uracil-DNA glycosylase isoform X1
XM_017594901.1	XP_017450390.1	single-strand selective monofunctional uracil-DNA glycosylase isoform X1
NM_177934.3	NP_808795.1	single-strand selective monofunctional uracil-DNA glycosylase
XM_017594900.1	XP_017450389.1	single-strand selective monofunctional uracil-DNA glycosylase isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SMUG1	XP_001135205.2
Canis lupus familiaris (dog)	SMUG1	XP_005636805.1
Gallus gallus (chicken)	SMUG1	XP_428577.3
Drosophila melanogaster (fruit fly)	CG5285	NP_650609.1
Homo sapiens (human)	SMUG1	NP_001230717.1
Macaca mulatta (Rhesus monkey)	SMUG1	XP_001109204.1
Rattus norvegicus (Norway rat)	Smug1	NP_808795.1
Xenopus tropicalis (tropical clawed frog)	smug1	NP_001090786.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002876	XP_312038.5
Bos taurus (cattle)	SMUG1	NP_001014958.1
Mus musculus (house mouse)	Smug1	NP_082161.2
Danio rerio (zebrafish)	LOC570901	XP_699524.5

Gene Ontology
Bibliography

Related articles in PubMed

Mammalian 5-formyluracil-DNA glycosylase. 1. Identification and characterization of a novel activity that releases 5-formyluracil from DNA.
Matsubara M, Masaoka A, Tanaka T, Miyano T, Kato N, Terato H, Ohyama Y, Iwai S, Ide H
Biochemistry42(17)4993-5002(2003 May)

Mammalian 5-formyluracil-DNA glycosylase. 2. Role of SMUG1 uracil-DNA glycosylase in repair of 5-formyluracil and other oxidized and deaminated base lesions.
Masaoka A, Matsubara M, Hasegawa R, Tanaka T, Kurisu S, Terato H, Ohyama Y, Karino N, Matsuda A, Ide H
Biochemistry42(17)5003-12(2003 May)

hUNG2 is the major repair enzyme for removal of uracil from U:A matches, U:G mismatches, and U in single-stranded DNA, with hSMUG1 as a broad specificity backup.
Kavli B, Sundheim O, Akbari M, Otterlei M, Nilsen H, Skorpen F, Aas PA, Hagen L, Krokan HE, Slupphaug G
The Journal of biological chemistry277(42)39926-36(2002 Oct)

Definitive identification of mammalian 5-hydroxymethyluracil DNA N-glycosylase activity as SMUG1.
Boorstein RJ, Cummings A Jr, Marenstein DR, Chan MK, Ma Y, Neubert TA, Brown SM, Teebor GW
The Journal of biological chemistry276(45)41991-7(2001 Nov)

Testicular protein Spag5 has similarity to mitotic spindle protein Deepest and binds outer dense fiber protein Odf1.
Shao X, Xue J, van der Hoorn FA
Molecular reproduction and development59(4)410-6(2001 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Smug1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Smug1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa04083	NM_177934.3 Latest version!	Rattus norvegicus single-strand-selective monofunctional uracil-DNA glycosylase 1 (Smug1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
ORa04083	XM_017594900.1 Latest version!	Rattus norvegicus single-strand-selective monofunctional uracil-DNA glycosylase 1 (Smug1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
ORa04083	XM_017594902.1 Latest version!	Rattus norvegicus single-strand-selective monofunctional uracil-DNA glycosylase 1 (Smug1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
ORa04083	XM_008765749.2 Latest version!	Rattus norvegicus single-strand-selective monofunctional uracil-DNA glycosylase 1 (Smug1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
ORa04083	XM_017594901.1 Latest version!	Rattus norvegicus single-strand-selective monofunctional uracil-DNA glycosylase 1 (Smug1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Smug1 ( NM_177934.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_808795.1 Rattus norvegicus single-strand-selective monofunctional uracil-DNA glycosylase 1 (Smug1), mRNA.

Price & Availability↑

CloneID	ORa04083
Clone ID Related Accession (Same CDS sequence)	XM_017594902.1 , XM_008765749.2 , XM_017594901.1 , NM_177934.3 , XM_017594900.1
Accession Version	NM_177934.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 837bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1532016000000
Organism	Rattus norvegicus(Norway rat)
Product	single-strand selective monofunctional uracil-DNA glycosylase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY191521.1. On Feb 21, 2013 this sequence version replaced NM_177934.2. ##Evidence-Data-START## Transcript exon combination :: AY191521.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCTGTGT CCCAGACTTT CCCACCGGGG CCTGCCCATG AGCCTGCCAG TGCCCTGATG 
GAGCCTTGCG CTCGAAGCTT AGCTGAGGGC TTCCTGGAGG AAGAGCTTCG GCTCAATGCT 
GAGCTGAGCC AGCTGCAGTT TCCAGAGCCT GTGGGTGTCA TCTACAACCC AGTGGATTAT 
GCTTGGGAGC CACACCGGAA CTACGTGACT CGCTACTGCC AAGGCCCCAA GGAAGTGCTG 
TTCCTGGGCA TGAATCCAGG ACCTTTTGGC ATGGCCCAAA CTGGGGTACC TTTTGGGGAA 
GTGAATGTGG TCCGGGACTG GTTGGGCATT GGGGGCTCTG TGCTGTCCCC TCCCCAAGAG 
CACCCTAAGC GACCAGTGTT GGGACTGGAG TGCCCGCAGT CAGAGGTGAG CGGAGCCCGA 
TTCTGGGGCT TTTTTCGGAC CCTCTGCGGA CAGCCTCAAG TCTTCTTCCG GCACTGCTTT 
GTCCACAATC TGTGTCCTCT ACTCTTCTTG GCTCCCAGCG GGCGAAACCT TACCCCGGCT 
GATCTGCCCG CCAAGCACCG GGAGCAGCTA CTGTCGATCT GCGACGCCGC CCTCTGCCGG 
CAGGTGCAGC TGCTAGGGGT GCGGCTGGTA GTGGGAGTGG GGCGGCTGGC AGAGCAGAGA 
GCTCGAAGAG CTCTAGCAGG GCTGACCCCG GAGGTGCAGG TGGAGGGGCT CCTGCATCCA 
TCTCCTCGAA GCCCGCAGGC CAACAAAGGC TGGGAAACAG CAGCCAGGGA AAGACTCCAA 
GAGTTGGGGC TGCTGCCTCT GCTAACGGAT GAGGGTTCAG TCAGGCCTAC ACCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_808795.1
CDS	193..1029
Translation	MAVSQTFPPGPAHEPASALMEPCARSLAEGFLEEELRLNAELSQLQFPEPVGVIYNPVDYAWEPHRNYVTRYCQGPKEVLFLGMNPGPFGMAQTGVPFGEVNVVRDWLGIGGSVLSPPQEHPKRPVLGLECPQSEVSGARFWGFFRTLCGQPQVFFRHCFVHNLCPLLFLAPSGRNLTPADLPAKHREQLLSICDAALCRQVQLLGVRLVVGVGRLAEQRARRALAGLTPEVQVEGLLHPSPRSPQANKGWETAARERLQELGLLPLLTDEGSVRPTP

Target ORF information:

RefSeq Version	NM_177934.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus single-strand-selective monofunctional uracil-DNA glycosylase 1 (Smug1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_177934.3

ORF Insert Sequence:

ATGGCTGTGT CCCAGACTTT CCCACCGGGG CCTGCCCATG AGCCTGCCAG TGCCCTGATG 
GAGCCTTGCG CTCGAAGCTT AGCTGAGGGC TTCCTGGAGG AAGAGCTTCG GCTCAATGCT 
GAGCTGAGCC AGCTGCAGTT TCCAGAGCCT GTGGGTGTCA TCTACAACCC AGTGGATTAT 
GCTTGGGAGC CACACCGGAA CTACGTGACT CGCTACTGCC AAGGCCCCAA GGAAGTGCTG 
TTCCTGGGCA TGAATCCAGG ACCTTTTGGC ATGGCCCAAA CTGGGGTACC TTTTGGGGAA 
GTGAATGTGG TCCGGGACTG GTTGGGCATT GGGGGCTCTG TGCTGTCCCC TCCCCAAGAG 
CACCCTAAGC GACCAGTGTT GGGACTGGAG TGCCCGCAGT CAGAGGTGAG CGGAGCCCGA 
TTCTGGGGCT TTTTTCGGAC CCTCTGCGGA CAGCCTCAAG TCTTCTTCCG GCACTGCTTT 
GTCCACAATC TGTGTCCTCT ACTCTTCTTG GCTCCCAGCG GGCGAAACCT TACCCCGGCT 
GATCTGCCCG CCAAGCACCG GGAGCAGCTA CTGTCGATCT GCGACGCCGC CCTCTGCCGG 
CAGGTGCAGC TGCTAGGGGT GCGGCTGGTA GTGGGAGTGG GGCGGCTGGC AGAGCAGAGA 
GCTCGAAGAG CTCTAGCAGG GCTGACCCCG GAGGTGCAGG TGGAGGGGCT CCTGCATCCA 
TCTCCTCGAA GCCCGCAGGC CAACAAAGGC TGGGAAACAG CAGCCAGGGA AAGACTCCAA 
GAGTTGGGGC TGCTGCCTCT GCTAACGGAT GAGGGTTCAG TCAGGCCTAC ACCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smug1 ( XM_017594902.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017450391.1 Rattus norvegicus single-strand-selective monofunctional uracil-DNA glycosylase 1 (Smug1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa04083
Clone ID Related Accession (Same CDS sequence)	XM_017594902.1 , XM_008765749.2 , XM_017594901.1 , NM_177934.3 , XM_017594900.1
Accession Version	XM_017594902.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 837bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	single-strand selective monofunctional uracil-DNA glycosylase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTGT CCCAGACTTT CCCACCGGGG CCTGCCCATG AGCCTGCCAG TGCCCTGATG 
GAGCCTTGCG CTCGAAGCTT AGCTGAGGGC TTCCTGGAGG AAGAGCTTCG GCTCAATGCT 
GAGCTGAGCC AGCTGCAGTT TCCAGAGCCT GTGGGTGTCA TCTACAACCC AGTGGATTAT 
GCTTGGGAGC CACACCGGAA CTACGTGACT CGCTACTGCC AAGGCCCCAA GGAAGTGCTG 
TTCCTGGGCA TGAATCCAGG ACCTTTTGGC ATGGCCCAAA CTGGGGTACC TTTTGGGGAA 
GTGAATGTGG TCCGGGACTG GTTGGGCATT GGGGGCTCTG TGCTGTCCCC TCCCCAAGAG 
CACCCTAAGC GACCAGTGTT GGGACTGGAG TGCCCGCAGT CAGAGGTGAG CGGAGCCCGA 
TTCTGGGGCT TTTTTCGGAC CCTCTGCGGA CAGCCTCAAG TCTTCTTCCG GCACTGCTTT 
GTCCACAATC TGTGTCCTCT ACTCTTCTTG GCTCCCAGCG GGCGAAACCT TACCCCGGCT 
GATCTGCCCG CCAAGCACCG GGAGCAGCTA CTGTCGATCT GCGACGCCGC CCTCTGCCGG 
CAGGTGCAGC TGCTAGGGGT GCGGCTGGTA GTGGGAGTGG GGCGGCTGGC AGAGCAGAGA 
GCTCGAAGAG CTCTAGCAGG GCTGACCCCG GAGGTGCAGG TGGAGGGGCT CCTGCATCCA 
TCTCCTCGAA GCCCGCAGGC CAACAAAGGC TGGGAAACAG CAGCCAGGGA AAGACTCCAA 
GAGTTGGGGC TGCTGCCTCT GCTAACGGAT GAGGGTTCAG TCAGGCCTAC ACCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017450391.1
CDS	1127..1963
Translation	MAVSQTFPPGPAHEPASALMEPCARSLAEGFLEEELRLNAELSQLQFPEPVGVIYNPVDYAWEPHRNYVTRYCQGPKEVLFLGMNPGPFGMAQTGVPFGEVNVVRDWLGIGGSVLSPPQEHPKRPVLGLECPQSEVSGARFWGFFRTLCGQPQVFFRHCFVHNLCPLLFLAPSGRNLTPADLPAKHREQLLSICDAALCRQVQLLGVRLVVGVGRLAEQRARRALAGLTPEVQVEGLLHPSPRSPQANKGWETAARERLQELGLLPLLTDEGSVRPTP

Target ORF information:

RefSeq Version	XM_017594902.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus single-strand-selective monofunctional uracil-DNA glycosylase 1 (Smug1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017594902.1

ORF Insert Sequence:

ATGGCTGTGT CCCAGACTTT CCCACCGGGG CCTGCCCATG AGCCTGCCAG TGCCCTGATG 
GAGCCTTGCG CTCGAAGCTT AGCTGAGGGC TTCCTGGAGG AAGAGCTTCG GCTCAATGCT 
GAGCTGAGCC AGCTGCAGTT TCCAGAGCCT GTGGGTGTCA TCTACAACCC AGTGGATTAT 
GCTTGGGAGC CACACCGGAA CTACGTGACT CGCTACTGCC AAGGCCCCAA GGAAGTGCTG 
TTCCTGGGCA TGAATCCAGG ACCTTTTGGC ATGGCCCAAA CTGGGGTACC TTTTGGGGAA 
GTGAATGTGG TCCGGGACTG GTTGGGCATT GGGGGCTCTG TGCTGTCCCC TCCCCAAGAG 
CACCCTAAGC GACCAGTGTT GGGACTGGAG TGCCCGCAGT CAGAGGTGAG CGGAGCCCGA 
TTCTGGGGCT TTTTTCGGAC CCTCTGCGGA CAGCCTCAAG TCTTCTTCCG GCACTGCTTT 
GTCCACAATC TGTGTCCTCT ACTCTTCTTG GCTCCCAGCG GGCGAAACCT TACCCCGGCT 
GATCTGCCCG CCAAGCACCG GGAGCAGCTA CTGTCGATCT GCGACGCCGC CCTCTGCCGG 
CAGGTGCAGC TGCTAGGGGT GCGGCTGGTA GTGGGAGTGG GGCGGCTGGC AGAGCAGAGA 
GCTCGAAGAG CTCTAGCAGG GCTGACCCCG GAGGTGCAGG TGGAGGGGCT CCTGCATCCA 
TCTCCTCGAA GCCCGCAGGC CAACAAAGGC TGGGAAACAG CAGCCAGGGA AAGACTCCAA 
GAGTTGGGGC TGCTGCCTCT GCTAACGGAT GAGGGTTCAG TCAGGCCTAC ACCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smug1 ( XM_008765749.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008763971.1 Rattus norvegicus single-strand-selective monofunctional uracil-DNA glycosylase 1 (Smug1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa04083
Clone ID Related Accession (Same CDS sequence)	XM_017594902.1 , XM_008765749.2 , XM_017594901.1 , NM_177934.3 , XM_017594900.1
Accession Version	XM_008765749.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 837bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	single-strand selective monofunctional uracil-DNA glycosylase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008765749.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTGT CCCAGACTTT CCCACCGGGG CCTGCCCATG AGCCTGCCAG TGCCCTGATG 
GAGCCTTGCG CTCGAAGCTT AGCTGAGGGC TTCCTGGAGG AAGAGCTTCG GCTCAATGCT 
GAGCTGAGCC AGCTGCAGTT TCCAGAGCCT GTGGGTGTCA TCTACAACCC AGTGGATTAT 
GCTTGGGAGC CACACCGGAA CTACGTGACT CGCTACTGCC AAGGCCCCAA GGAAGTGCTG 
TTCCTGGGCA TGAATCCAGG ACCTTTTGGC ATGGCCCAAA CTGGGGTACC TTTTGGGGAA 
GTGAATGTGG TCCGGGACTG GTTGGGCATT GGGGGCTCTG TGCTGTCCCC TCCCCAAGAG 
CACCCTAAGC GACCAGTGTT GGGACTGGAG TGCCCGCAGT CAGAGGTGAG CGGAGCCCGA 
TTCTGGGGCT TTTTTCGGAC CCTCTGCGGA CAGCCTCAAG TCTTCTTCCG GCACTGCTTT 
GTCCACAATC TGTGTCCTCT ACTCTTCTTG GCTCCCAGCG GGCGAAACCT TACCCCGGCT 
GATCTGCCCG CCAAGCACCG GGAGCAGCTA CTGTCGATCT GCGACGCCGC CCTCTGCCGG 
CAGGTGCAGC TGCTAGGGGT GCGGCTGGTA GTGGGAGTGG GGCGGCTGGC AGAGCAGAGA 
GCTCGAAGAG CTCTAGCAGG GCTGACCCCG GAGGTGCAGG TGGAGGGGCT CCTGCATCCA 
TCTCCTCGAA GCCCGCAGGC CAACAAAGGC TGGGAAACAG CAGCCAGGGA AAGACTCCAA 
GAGTTGGGGC TGCTGCCTCT GCTAACGGAT GAGGGTTCAG TCAGGCCTAC ACCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008763971.1
CDS	1226..2062
Translation	MAVSQTFPPGPAHEPASALMEPCARSLAEGFLEEELRLNAELSQLQFPEPVGVIYNPVDYAWEPHRNYVTRYCQGPKEVLFLGMNPGPFGMAQTGVPFGEVNVVRDWLGIGGSVLSPPQEHPKRPVLGLECPQSEVSGARFWGFFRTLCGQPQVFFRHCFVHNLCPLLFLAPSGRNLTPADLPAKHREQLLSICDAALCRQVQLLGVRLVVGVGRLAEQRARRALAGLTPEVQVEGLLHPSPRSPQANKGWETAARERLQELGLLPLLTDEGSVRPTP

Target ORF information:

RefSeq Version	XM_008765749.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus single-strand-selective monofunctional uracil-DNA glycosylase 1 (Smug1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008765749.2

ORF Insert Sequence:

ATGGCTGTGT CCCAGACTTT CCCACCGGGG CCTGCCCATG AGCCTGCCAG TGCCCTGATG 
GAGCCTTGCG CTCGAAGCTT AGCTGAGGGC TTCCTGGAGG AAGAGCTTCG GCTCAATGCT 
GAGCTGAGCC AGCTGCAGTT TCCAGAGCCT GTGGGTGTCA TCTACAACCC AGTGGATTAT 
GCTTGGGAGC CACACCGGAA CTACGTGACT CGCTACTGCC AAGGCCCCAA GGAAGTGCTG 
TTCCTGGGCA TGAATCCAGG ACCTTTTGGC ATGGCCCAAA CTGGGGTACC TTTTGGGGAA 
GTGAATGTGG TCCGGGACTG GTTGGGCATT GGGGGCTCTG TGCTGTCCCC TCCCCAAGAG 
CACCCTAAGC GACCAGTGTT GGGACTGGAG TGCCCGCAGT CAGAGGTGAG CGGAGCCCGA 
TTCTGGGGCT TTTTTCGGAC CCTCTGCGGA CAGCCTCAAG TCTTCTTCCG GCACTGCTTT 
GTCCACAATC TGTGTCCTCT ACTCTTCTTG GCTCCCAGCG GGCGAAACCT TACCCCGGCT 
GATCTGCCCG CCAAGCACCG GGAGCAGCTA CTGTCGATCT GCGACGCCGC CCTCTGCCGG 
CAGGTGCAGC TGCTAGGGGT GCGGCTGGTA GTGGGAGTGG GGCGGCTGGC AGAGCAGAGA 
GCTCGAAGAG CTCTAGCAGG GCTGACCCCG GAGGTGCAGG TGGAGGGGCT CCTGCATCCA 
TCTCCTCGAA GCCCGCAGGC CAACAAAGGC TGGGAAACAG CAGCCAGGGA AAGACTCCAA 
GAGTTGGGGC TGCTGCCTCT GCTAACGGAT GAGGGTTCAG TCAGGCCTAC ACCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smug1 ( XM_017594901.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017450390.1 Rattus norvegicus single-strand-selective monofunctional uracil-DNA glycosylase 1 (Smug1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa04083
Clone ID Related Accession (Same CDS sequence)	XM_017594902.1 , XM_008765749.2 , XM_017594901.1 , NM_177934.3 , XM_017594900.1
Accession Version	XM_017594901.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 837bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	single-strand selective monofunctional uracil-DNA glycosylase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTGT CCCAGACTTT CCCACCGGGG CCTGCCCATG AGCCTGCCAG TGCCCTGATG 
GAGCCTTGCG CTCGAAGCTT AGCTGAGGGC TTCCTGGAGG AAGAGCTTCG GCTCAATGCT 
GAGCTGAGCC AGCTGCAGTT TCCAGAGCCT GTGGGTGTCA TCTACAACCC AGTGGATTAT 
GCTTGGGAGC CACACCGGAA CTACGTGACT CGCTACTGCC AAGGCCCCAA GGAAGTGCTG 
TTCCTGGGCA TGAATCCAGG ACCTTTTGGC ATGGCCCAAA CTGGGGTACC TTTTGGGGAA 
GTGAATGTGG TCCGGGACTG GTTGGGCATT GGGGGCTCTG TGCTGTCCCC TCCCCAAGAG 
CACCCTAAGC GACCAGTGTT GGGACTGGAG TGCCCGCAGT CAGAGGTGAG CGGAGCCCGA 
TTCTGGGGCT TTTTTCGGAC CCTCTGCGGA CAGCCTCAAG TCTTCTTCCG GCACTGCTTT 
GTCCACAATC TGTGTCCTCT ACTCTTCTTG GCTCCCAGCG GGCGAAACCT TACCCCGGCT 
GATCTGCCCG CCAAGCACCG GGAGCAGCTA CTGTCGATCT GCGACGCCGC CCTCTGCCGG 
CAGGTGCAGC TGCTAGGGGT GCGGCTGGTA GTGGGAGTGG GGCGGCTGGC AGAGCAGAGA 
GCTCGAAGAG CTCTAGCAGG GCTGACCCCG GAGGTGCAGG TGGAGGGGCT CCTGCATCCA 
TCTCCTCGAA GCCCGCAGGC CAACAAAGGC TGGGAAACAG CAGCCAGGGA AAGACTCCAA 
GAGTTGGGGC TGCTGCCTCT GCTAACGGAT GAGGGTTCAG TCAGGCCTAC ACCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017450390.1
CDS	1044..1880
Translation	MAVSQTFPPGPAHEPASALMEPCARSLAEGFLEEELRLNAELSQLQFPEPVGVIYNPVDYAWEPHRNYVTRYCQGPKEVLFLGMNPGPFGMAQTGVPFGEVNVVRDWLGIGGSVLSPPQEHPKRPVLGLECPQSEVSGARFWGFFRTLCGQPQVFFRHCFVHNLCPLLFLAPSGRNLTPADLPAKHREQLLSICDAALCRQVQLLGVRLVVGVGRLAEQRARRALAGLTPEVQVEGLLHPSPRSPQANKGWETAARERLQELGLLPLLTDEGSVRPTP

Target ORF information:

RefSeq Version	XM_017594901.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus single-strand-selective monofunctional uracil-DNA glycosylase 1 (Smug1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017594901.1

ORF Insert Sequence:

ATGGCTGTGT CCCAGACTTT CCCACCGGGG CCTGCCCATG AGCCTGCCAG TGCCCTGATG 
GAGCCTTGCG CTCGAAGCTT AGCTGAGGGC TTCCTGGAGG AAGAGCTTCG GCTCAATGCT 
GAGCTGAGCC AGCTGCAGTT TCCAGAGCCT GTGGGTGTCA TCTACAACCC AGTGGATTAT 
GCTTGGGAGC CACACCGGAA CTACGTGACT CGCTACTGCC AAGGCCCCAA GGAAGTGCTG 
TTCCTGGGCA TGAATCCAGG ACCTTTTGGC ATGGCCCAAA CTGGGGTACC TTTTGGGGAA 
GTGAATGTGG TCCGGGACTG GTTGGGCATT GGGGGCTCTG TGCTGTCCCC TCCCCAAGAG 
CACCCTAAGC GACCAGTGTT GGGACTGGAG TGCCCGCAGT CAGAGGTGAG CGGAGCCCGA 
TTCTGGGGCT TTTTTCGGAC CCTCTGCGGA CAGCCTCAAG TCTTCTTCCG GCACTGCTTT 
GTCCACAATC TGTGTCCTCT ACTCTTCTTG GCTCCCAGCG GGCGAAACCT TACCCCGGCT 
GATCTGCCCG CCAAGCACCG GGAGCAGCTA CTGTCGATCT GCGACGCCGC CCTCTGCCGG 
CAGGTGCAGC TGCTAGGGGT GCGGCTGGTA GTGGGAGTGG GGCGGCTGGC AGAGCAGAGA 
GCTCGAAGAG CTCTAGCAGG GCTGACCCCG GAGGTGCAGG TGGAGGGGCT CCTGCATCCA 
TCTCCTCGAA GCCCGCAGGC CAACAAAGGC TGGGAAACAG CAGCCAGGGA AAGACTCCAA 
GAGTTGGGGC TGCTGCCTCT GCTAACGGAT GAGGGTTCAG TCAGGCCTAC ACCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smug1 ( XM_017594900.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017450389.1 Rattus norvegicus single-strand-selective monofunctional uracil-DNA glycosylase 1 (Smug1), transcript variant X1, mRNA.

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CloneID	ORa04083
Clone ID Related Accession (Same CDS sequence)	XM_017594902.1 , XM_008765749.2 , XM_017594901.1 , NM_177934.3 , XM_017594900.1
Accession Version	XM_017594900.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 837bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	single-strand selective monofunctional uracil-DNA glycosylase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTGT CCCAGACTTT CCCACCGGGG CCTGCCCATG AGCCTGCCAG TGCCCTGATG 
GAGCCTTGCG CTCGAAGCTT AGCTGAGGGC TTCCTGGAGG AAGAGCTTCG GCTCAATGCT 
GAGCTGAGCC AGCTGCAGTT TCCAGAGCCT GTGGGTGTCA TCTACAACCC AGTGGATTAT 
GCTTGGGAGC CACACCGGAA CTACGTGACT CGCTACTGCC AAGGCCCCAA GGAAGTGCTG 
TTCCTGGGCA TGAATCCAGG ACCTTTTGGC ATGGCCCAAA CTGGGGTACC TTTTGGGGAA 
GTGAATGTGG TCCGGGACTG GTTGGGCATT GGGGGCTCTG TGCTGTCCCC TCCCCAAGAG 
CACCCTAAGC GACCAGTGTT GGGACTGGAG TGCCCGCAGT CAGAGGTGAG CGGAGCCCGA 
TTCTGGGGCT TTTTTCGGAC CCTCTGCGGA CAGCCTCAAG TCTTCTTCCG GCACTGCTTT 
GTCCACAATC TGTGTCCTCT ACTCTTCTTG GCTCCCAGCG GGCGAAACCT TACCCCGGCT 
GATCTGCCCG CCAAGCACCG GGAGCAGCTA CTGTCGATCT GCGACGCCGC CCTCTGCCGG 
CAGGTGCAGC TGCTAGGGGT GCGGCTGGTA GTGGGAGTGG GGCGGCTGGC AGAGCAGAGA 
GCTCGAAGAG CTCTAGCAGG GCTGACCCCG GAGGTGCAGG TGGAGGGGCT CCTGCATCCA 
TCTCCTCGAA GCCCGCAGGC CAACAAAGGC TGGGAAACAG CAGCCAGGGA AAGACTCCAA 
GAGTTGGGGC TGCTGCCTCT GCTAACGGAT GAGGGTTCAG TCAGGCCTAC ACCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017450389.1
CDS	1296..2132
Translation	MAVSQTFPPGPAHEPASALMEPCARSLAEGFLEEELRLNAELSQLQFPEPVGVIYNPVDYAWEPHRNYVTRYCQGPKEVLFLGMNPGPFGMAQTGVPFGEVNVVRDWLGIGGSVLSPPQEHPKRPVLGLECPQSEVSGARFWGFFRTLCGQPQVFFRHCFVHNLCPLLFLAPSGRNLTPADLPAKHREQLLSICDAALCRQVQLLGVRLVVGVGRLAEQRARRALAGLTPEVQVEGLLHPSPRSPQANKGWETAARERLQELGLLPLLTDEGSVRPTP

Target ORF information:

RefSeq Version	XM_017594900.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus single-strand-selective monofunctional uracil-DNA glycosylase 1 (Smug1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017594900.1

ORF Insert Sequence:

ATGGCTGTGT CCCAGACTTT CCCACCGGGG CCTGCCCATG AGCCTGCCAG TGCCCTGATG 
GAGCCTTGCG CTCGAAGCTT AGCTGAGGGC TTCCTGGAGG AAGAGCTTCG GCTCAATGCT 
GAGCTGAGCC AGCTGCAGTT TCCAGAGCCT GTGGGTGTCA TCTACAACCC AGTGGATTAT 
GCTTGGGAGC CACACCGGAA CTACGTGACT CGCTACTGCC AAGGCCCCAA GGAAGTGCTG 
TTCCTGGGCA TGAATCCAGG ACCTTTTGGC ATGGCCCAAA CTGGGGTACC TTTTGGGGAA 
GTGAATGTGG TCCGGGACTG GTTGGGCATT GGGGGCTCTG TGCTGTCCCC TCCCCAAGAG 
CACCCTAAGC GACCAGTGTT GGGACTGGAG TGCCCGCAGT CAGAGGTGAG CGGAGCCCGA 
TTCTGGGGCT TTTTTCGGAC CCTCTGCGGA CAGCCTCAAG TCTTCTTCCG GCACTGCTTT 
GTCCACAATC TGTGTCCTCT ACTCTTCTTG GCTCCCAGCG GGCGAAACCT TACCCCGGCT 
GATCTGCCCG CCAAGCACCG GGAGCAGCTA CTGTCGATCT GCGACGCCGC CCTCTGCCGG 
CAGGTGCAGC TGCTAGGGGT GCGGCTGGTA GTGGGAGTGG GGCGGCTGGC AGAGCAGAGA 
GCTCGAAGAG CTCTAGCAGG GCTGACCCCG GAGGTGCAGG TGGAGGGGCT CCTGCATCCA 
TCTCCTCGAA GCCCGCAGGC CAACAAAGGC TGGGAAACAG CAGCCAGGGA AAGACTCCAA 
GAGTTGGGGC TGCTGCCTCT GCTAACGGAT GAGGGTTCAG TCAGGCCTAC ACCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mammalian 5-formyluracil-DNA glycosylase. 1. Identification and characterization of a novel activity that releases 5-formyluracil from DNA.
Biochemistry42(17)4993-5002(2003 May)
Matsubara M,Masaoka A,Tanaka T,Miyano T,Kato N,Terato H,Ohyama Y,Iwai S,Ide H

Mammalian 5-formyluracil-DNA glycosylase. 2. Role of SMUG1 uracil-DNA glycosylase in repair of 5-formyluracil and other oxidized and deaminated base lesions.
Biochemistry42(17)5003-12(2003 May)
Masaoka A,Matsubara M,Hasegawa R,Tanaka T,Kurisu S,Terato H,Ohyama Y,Karino N,Matsuda A,Ide H

hUNG2 is the major repair enzyme for removal of uracil from U:A matches, U:G mismatches, and U in single-stranded DNA, with hSMUG1 as a broad specificity backup.
The Journal of biological chemistry277(42)39926-36(2002 Oct)
Kavli B,Sundheim O,Akbari M,Otterlei M,Nilsen H,Skorpen F,Aas PA,Hagen L,Krokan HE,Slupphaug G

Definitive identification of mammalian 5-hydroxymethyluracil DNA N-glycosylase activity as SMUG1.
The Journal of biological chemistry276(45)41991-7(2001 Nov)
Boorstein RJ,Cummings A Jr,Marenstein DR,Chan MK,Ma Y,Neubert TA,Brown SM,Teebor GW

Testicular protein Spag5 has similarity to mitotic spindle protein Deepest and binds outer dense fiber protein Odf1.
Molecular reproduction and development59(4)410-6(2001 Aug)
Shao X,Xue J,van der Hoorn FA

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Smug1 cDNA ORF clone, Rattus norvegicus(Norway rat)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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