Focad cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Focad
Entrez Gene ID	313346
Full Name	focadhesin
Synonyms	Ir22,RGD1311849
General protein information	Preferred Names focadhesin Names focadhesin
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 5 Map Location: 5q32

mRNA and Protein(s)

mRNA	Protein	Name
XM_017603024.1	XP_017458513.1	focadhesin isoform X1
XM_017603022.1	XP_017458511.1	focadhesin isoform X2
XM_001053590.5	XP_001053590.1	focadhesin isoform X2
XM_233144.8	XP_233144.5	focadhesin isoform X2
XM_017593754.1	XP_017449243.1	focadhesin isoform X1
XM_017603023.1	XP_017458512.1	focadhesin isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	FOCAD	NP_060264.3
Bos taurus (cattle)	FOCAD	NP_001193444.1
Rattus norvegicus (Norway rat)	Focad	XP_233144.5
Pan troglodytes (chimpanzee)	FOCAD	XP_001151442.1
Macaca mulatta (Rhesus monkey)	LOC711490	XP_002800166.1
Mus musculus (house mouse)	Focad	NP_001074653.1
Canis lupus familiaris (dog)	FOCAD	XP_538678.2
Gallus gallus (chicken)	FOCAD	NP_001182532.1
Danio rerio (zebrafish)	focad	XP_003198980.1

Gene Ontology

The following Focad gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Focad cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa16728	XM_017603022.1 Latest version!	Rattus norvegicus focadhesin (Focad), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ORa16728	XM_233144.8 Latest version!	Rattus norvegicus focadhesin (Focad), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ORa16728	XM_001053590.5 Latest version!	Rattus norvegicus focadhesin (Focad), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ORa16728	XM_017603023.1 Latest version!	Rattus norvegicus focadhesin (Focad), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ORa55473	XM_017603024.1 Latest version!	Rattus norvegicus focadhesin (Focad), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ORa55473	XM_017593754.1 Latest version!	Rattus norvegicus focadhesin (Focad), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Focad ( XM_017603022.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017458511.1 Rattus norvegicus focadhesin (Focad), transcript variant X3, mRNA.

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CloneID	ORa16728
Clone ID Related Accession (Same CDS sequence)	XM_017603022.1 , XM_001053590.5 , XM_233144.8 , XM_017603023.1
Accession Version	XM_017603022.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5397bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	focadhesin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000073.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGATG ATATCAAGAA AAGGTTTGAT TTTCCGAATT CCCTTATCCA GTCCCAGGCT 
GTAGGTCATC TTATTGCTGC TGTCTTAAAA GAACATGGCG CTTCAGAAAA GATACACCAG 
TCCACAAACC AGACTGCTGC CTTGAACTTG CTGTGGGAGA AGAGCTGCAG TGACAATGTG 
GTGGTCCGAA CAGCCTGCAG CGAGGGACTG GTGGCACTTG TTGCTCAGGG TCACGCAGAG 
TTCAGCTATG TTCTCAATGG GATACTCAAC CTGATTCCTT CTGCCAGGAA TATGTCCGGT 
TTGATAAAAG CCCTTATGAA ATTATTACAA ATGCAAGCAT CCAGGGAAGG ACAAGGTGGG 
GCAAAGGATC TTCAGGACCT ATATTCTATC AGAAATCCTC CTCAGCCTTT GATCACCGTG 
CTTGAGCACA GGCCTGATTG CTGGCCACTA CTCCTGCAGC AGCTGACAGC TTTTTTCCAG 
CAATGCCCTG AAAGGTCAGA AGCTTCATGT GTCCAAATAA TGGCACCATT TCTGTGGTAT 
CTCTACTGTG AACCATCCCA GTTACAAGAA TATGCTAAGC TTCGACTGTC CCTGCTCAAA 
GTCTTGCTTC AGCCCAGAGG TGTTTGTGAG GAAGCACAGC CATCAGTGTT GGAACAGCAG 
ATCCTTCAGC TGTGCTGTAA CATGGTTCCG TGCTTGCAGA TGAAAGACCT GATGCAGACA 
ACAGAGATGA CGCTGTTCGT GGAGGAAGTA TATCTAAGCC TTCTGCGCCA TCCTGCTTTC 
TGGAAGAGAC AGCTTACCCA GCTGACCGTT CAGTTGCTGT GCATCTGTGA AGTCAGCTTA 
AAGACAACTG GGGAATGCTC ATCTTTAATT CAGCTTTTAG AGCGCAGTGT TGAACTTCTT 
GGGGAGGATT TTCCTGTCAA ATTGGTCATT ATTGGAATAG CTTTAATACT TCTACAGACT 
CCAGCAAGTC AGCAGAAGCC AGTATTGAAC CTAACTCTGA AGCTCCTCTC GCTTACTGAG 
GGTCAGAGAA TCCCGAAGGC CTCTCTGCTG CTCCTGGTGC CTCTTCTGCA GATTCTGTCC 
TCCACTGCCT TGGAAGACTG TCTGGCCACG GGTGAGGAAG GCCCCTCCAG GCAGCAGTTA 
GCCCTCAGTC TGTTGGAAGT GCTACAGAGA GAGTGTCATG GCGACGACCG ACACACGATC 
TCCTGCAGGC TTGCTTTTCC TGTGAGCAGC ATGTATGGCT CAGTCTTCAC TGCCTGCAGG 
ATTCTTGAAG TCATGGGAGA AGCGTCCGCA GCGAGTGACT GGCTGGCTGC AGTAGAGTCC 
TTGCTCCCTG CCACCACAGT GATCCCCCCA CATGTTTTCT TACTGCTGGC TCACCTGCTT 
GTTGAAGATG GAGGACAGAA TCTTCAGCAG ATACTGAAGG TCACCACACA GTTAGCTCAA 
GCAGATTCCT CTCAGGTACC ACATCTGATT CCAGTTCTGA TGTTCAAATT GGGGAGACCC 
TTAGACCCTG TATCATATAA TCATATATTA TATACTTTGC CCACACTTGG TGTTCACAAG 
GTATGCGTAG GGCAGATTCT GCGGGTGATT CAACTGCTGG GGACCACACC ACGACTAAGA 
GCTGTCACGT TACGCCTGTT GACATCTCTG TGGGAAAAGC AGGATCGTGT GTACCCTGAA 
CTGCAGCGCT TCATGGCCAT GTCTGATGCA CCCTCTCTCT CTGTGGGCAA GGAGCTGCAG 
TGGGAGAAAC TGATTGCAAA AGCTGCATCC ATCAGAGACA TATGTAAACA GAGGCCCTAT 
CAGCATGGTG CAGACATGCT GGCAGCCATT TCTCAGGTGT TGAACGAATG CACCAAGCCT 
GATCAAGCTT CTCCAGCTGC TTTGGTGTTA CAAGGTCTTC ATGCACTCTG CCAGGCTGAG 
GTTGTCTGTA TTCGCTCCAC ATGGAATGCT CTCTCTCCCA AGTTGAGCTG TGACATGAGA 
CCACTTATAT TGAAGACCCT GAGTGAACTA TTCTCTCTGG TTCCTTCCTT AACAGTCAAT 
ACAGTTGAAT ATGAGAATTT TAAAGTTCAA GTCCTCACCT TCCTCTGGAC TCACACTCAA 
AACAAGGATC CCATTGTAGC AAGTGCCGCA TATAAATCTC TGTCTCACTT CAGTGCTGGA 
GAGCACACCA TTCTTCACCT GCCAGAAAAG ATAAGGCCAG AAATGCCGGT TCCCGATGAG 
TTGGATGAGG AGGAATCTGT GGACCTTTCC ATCCCTGGCT CTTGCTATCT CCGACTGCTG 
ACCATCACTG CTCCTTCAAT TTTACCAGCT TTGGAAGAAT TCTTCACGTC TCTTGTGAGA 
CAAGAGATGG TGAATATGCC CCGTGGGATA TACCACTCAG CATTGAAGGG AGGCGTCTGC 
TCAGACCAAG GGAAGACAGT AGCAGGAATC CCCAACTTTA TATTGAAGAC ATATGAAACA 
AACAAGCAAC CAGGACTGAA ACCCGGTCTG GCAGGTGGCA TGCTGTTTTG CTATGATGTG 
GCCATGTATC AGAGCAAAGA TGGTAAGCCA CTGAATCGGC TGATGGCCAG TAGAGGGCGA 
ACCTTCAAGC AGACAACACT GGCCCTTGTT CATGAAGTCC ACGTCCAGCT CTCAGAATGG 
CACCGTGCAA TCTTTCTTCC ACAGGCTTGG CTTGCATACA TGACTCGAGC TTACCATGCC 
ATTTTACAGG GACGGATAGC AGAGCTGGAG CTGCAGTTGC AACATGGCAA GGAGGGCCCC 
GAGGAGGTGC AGTACAAACG AAGCACGGCC TGGCTCTGGG TCAGAGACAT GCTGACAGAT 
GAGATCACCA AGACAGCTGC GAAAGAGAGT CCAGTGGTCA AAGGCAATGC TCTGTTGGCC 
TTAAGCAGCC TTGCCGTCGT GGTGTCCAAG CATGAAGCCA GCCTTTCCTC AGAGTCTGAT 
GGAGTCCTAG AGGTCCAACC TAATTTCCTT CCAGTGAAAG AGTGGGTTTC CATGGTGCTT 
AATACTGTCC TGGTCATTGT GGATAGCCAT TACCACCCCA GAGGTCAACT GTTCACCTGC 
TTTTATCATA AGTCCTATTC TGGCGAGAAC ACGGCAAGTG CTATTGCGCG CTCTGCCGCC 
GCCACGGCTT TGTCTCTCCT CGTGCCGGTT TTCATTATCT CTTGCAAAGA GAAGGTTGAG 
GAAATCCTGA ACATGCTGAC TGCCAGGTTA CCTGGGAAAC CAAGTGCTGA TGAATCTCAA 
GCCGTGCAAA TCCACATGGG CCTTGCCTTG GGGATGTTTC TCTCTCGCCT GTGTGAGGAG 
AAACTCAGTG ACATGTCTGG CCAACAGATG AGCCTTCTTC TGATGAAGTC CTTGGATGCC 
CTGGAGAGCT GCTGTTTCGA CCCCAGTCTT GAACACAACA CCGGCTGTAT ACTGGGAGTT 
GGACTTGCCC TGTCCCTCAT GAGCCACAGC AGCCACACAG AGTCACGCGT TCATGTGGCA 
GCATCCCTGC GGAAGCTGTC TGCCTACCTG GATGAGAGCG GGAGCCAGAG CAGGACATTT 
CAGGAGGTCC TTGCCTACAC ACTTAGCTGT GTGTGTACAT CAGCGTTCAG TGCTGGCATT 
ATTGAGGCTG TGGAGGCTGA AGAGACTATG ACCAAGCTCC AGCTGTTGGT GGAGAACAAC 
CAGCAGACTT CAGGCTTTGC ACTGGCCTTA GGAAACTTGG TTTATGGGTT GTCTGTGTGT 
GGACATGGAA AAGCTGAAGA CTTGGGCAAC AGACTTCGCC CCAGCTGGAT TAAAATTGTC 
CTGTCAGAGG GTGCCCCCAC CATGCTCTGT CTGGCAGCCC TTCATGGCCT GGTGGCACTG 
GTAGGCTCCG ATGTGGATGT CATGCAGTTC CAGTCAGAAG CCATCCACAA CTCCCAGTTT 
CAAGCAAGAC TTAATGAAGT CATTCGAACC TTAACTGAGG TCATCAGTGT CTCAGGGGTG 
ATTGGTCTTC AATCAAATGC AATATGGCTG CTAGGACATC TTCATCTATC CACCTTGTCT 
TCAAATCAAA GCAGAACTTC CGTTCCTACC GACTACAGCT ATTTGCCTGA AGGCAGTTTT 
ATTAGAGCAG CCATTGGCTT CTTCATCACA GGAGGAAAAA AAGGCCCTGA AGCTGTGCCA 
CCGTCCCTTC TCAAAGTAGT GATGAAACCC ATAGCAACTG TTGGGGAAAA CTACCAGTAC 
CCTCCTGCAA ACTTAGCCGC ACTCCTCTCT CCACTTATGA GGCTAAACTT TGGCGAGGAG 
ATACAGCAAC TGTGCCTTGA GATTGCTGTG TCCCAGGCAC CGTCGTCACA GAGTGCAGCT 
GCACTGCTGG GCTTGTGGGT GATGCCGCCG CTGGTCCACG GTCTGAGTCT GAATATCAAG 
AAATACCTCC TGGTATCTAT GCCTCTGTGG GCAAAGCATG TTTCTGATGA ACAGATCCAG 
GGTTTTGTTG AAAATCTGAT GGTGGCAGTT TTTAAACCGG CTTCCCAACC TTGTCATCCT 
GAGATGTGCC CAAGTGCCTT ACAGGGTCTG AGCCAGGCCA TGAAACTGCC CAGTCCTTCC 
CACCACCTCT GGAGTCTGCT CTGCGATGCT ACTGGGAGAA TTTTTGACCT TCTGCCAAAT 
AGGATTCGGA GAAATGATCT CGAGCTGTAC ATCAGTATAG CAAAGTGCCT CTCAGAGATG 
ACGGATGAAG GTGCCAATCA GGTTTCCCAG ATTACTAAGG AAAACATAGA AAAGGCTGCC 
TTTGTCAAGC TGTACTTAAT CTCTCAAGGA CGACTCCCGT TGATGAGCCT GACTGATTTA 
CTCACTGCTG CTATGCAACA CCCCTCGAAG GAGACTCTAG CCTGGATGAT CCTGCACAGC 
TTATACCAGG CACGCATTGT CAATCACACC AACACAGGTG TACTGAAGAG ACTGGAGTGG 
CTCCTGGAGC TGATGGGTTA TATGAGAAAC ATTGCTTACC AGTCGGCGTC TGCTCAGAAT 
GTGCCTCCTG CGGAGGCTTT GGACTTCTTG ATGCTGATAT TTGCTGCTGC AGTGGTAGCA 
TGGGCAGACC ATGAGGCCCC CCTTCTCCTT GGCCTCAGTG CCAGTTGGTT GCCATGGCAC 
CAGGAAAATG GCCCTGGTGG GCCAGCAGCA GCCTTACTTG GCAGGAGTCC GATGCACAGA 
GTCACTGTGC AGGAGGTTCT CACTCTCCTC CCCACGAGCA TGCTACTGCT CCTGCAAAAA 
GAGCCATGGA AGGAACAGAC CCAGAAGTTC ATTGACTGGC TGTTCAGCAT CATGGAAATT 
CCTAACGAAG CCTTTGCAGC CACGTCCAAG GATCTTTTGA AAGCCACCCT GCTGTCCTTG 
AGAGTTCTCC CAGAGTTTAA GAAGAAAGCT GTATGGACCC GAGCGTATGG CTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017458511.1
CDS	293..5689
Translation	MSDDIKKRFDFPNSLIQSQAVGHLIAAVLKEHGASEKIHQSTNQTAALNLLWEKSCSDNVVVRTACSEGLVALVAQGHAEFSYVLNGILNLIPSARNMSGLIKALMKLLQMQASREGQGGAKDLQDLYSIRNPPQPLITVLEHRPDCWPLLLQQLTAFFQQCPERSEASCVQIMAPFLWYLYCEPSQLQEYAKLRLSLLKVLLQPRGVCEEAQPSVLEQQILQLCCNMVPCLQMKDLMQTTEMTLFVEEVYLSLLRHPAFWKRQLTQLTVQLLCICEVSLKTTGECSSLIQLLERSVELLGEDFPVKLVIIGIALILLQTPASQQKPVLNLTLKLLSLTEGQRIPKASLLLLVPLLQILSSTALEDCLATGEEGPSRQQLALSLLEVLQRECHGDDRHTISCRLAFPVSSMYGSVFTACRILEVMGEASAASDWLAAVESLLPATTVIPPHVFLLLAHLLVEDGGQNLQQILKVTTQLAQADSSQVPHLIPVLMFKLGRPLDPVSYNHILYTLPTLGVHKVCVGQILRVIQLLGTTPRLRAVTLRLLTSLWEKQDRVYPELQRFMAMSDAPSLSVGKELQWEKLIAKAASIRDICKQRPYQHGADMLAAISQVLNECTKPDQASPAALVLQGLHALCQAEVVCIRSTWNALSPKLSCDMRPLILKTLSELFSLVPSLTVNTVEYENFKVQVLTFLWTHTQNKDPIVASAAYKSLSHFSAGEHTILHLPEKIRPEMPVPDELDEEESVDLSIPGSCYLRLLTITAPSILPALEEFFTSLVRQEMVNMPRGIYHSALKGGVCSDQGKTVAGIPNFILKTYETNKQPGLKPGLAGGMLFCYDVAMYQSKDGKPLNRLMASRGRTFKQTTLALVHEVHVQLSEWHRAIFLPQAWLAYMTRAYHAILQGRIAELELQLQHGKEGPEEVQYKRSTAWLWVRDMLTDEITKTAAKESPVVKGNALLALSSLAVVVSKHEASLSSESDGVLEVQPNFLPVKEWVSMVLNTVLVIVDSHYHPRGQLFTCFYHKSYSGENTASAIARSAAATALSLLVPVFIISCKEKVEEILNMLTARLPGKPSADESQAVQIHMGLALGMFLSRLCEEKLSDMSGQQMSLLLMKSLDALESCCFDPSLEHNTGCILGVGLALSLMSHSSHTESRVHVAASLRKLSAYLDESGSQSRTFQEVLAYTLSCVCTSAFSAGIIEAVEAEETMTKLQLLVENNQQTSGFALALGNLVYGLSVCGHGKAEDLGNRLRPSWIKIVLSEGAPTMLCLAALHGLVALVGSDVDVMQFQSEAIHNSQFQARLNEVIRTLTEVISVSGVIGLQSNAIWLLGHLHLSTLSSNQSRTSVPTDYSYLPEGSFIRAAIGFFITGGKKGPEAVPPSLLKVVMKPIATVGENYQYPPANLAALLSPLMRLNFGEEIQQLCLEIAVSQAPSSQSAAALLGLWVMPPLVHGLSLNIKKYLLVSMPLWAKHVSDEQIQGFVENLMVAVFKPASQPCHPEMCPSALQGLSQAMKLPSPSHHLWSLLCDATGRIFDLLPNRIRRNDLELYISIAKCLSEMTDEGANQVSQITKENIEKAAFVKLYLISQGRLPLMSLTDLLTAAMQHPSKETLAWMILHSLYQARIVNHTNTGVLKRLEWLLELMGYMRNIAYQSASAQNVPPAEALDFLMLIFAAAVVAWADHEAPLLLGLSASWLPWHQENGPGGPAAALLGRSPMHRVTVQEVLTLLPTSMLLLLQKEPWKEQTQKFIDWLFSIMEIPNEAFAATSKDLLKATLLSLRVLPEFKKKAVWTRAYGW

Target ORF information:

RefSeq Version	XM_017603022.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus focadhesin (Focad), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017603022.1

ORF Insert Sequence:

ATGTCAGATG ATATCAAGAA AAGGTTTGAT TTTCCGAATT CCCTTATCCA GTCCCAGGCT 
GTAGGTCATC TTATTGCTGC TGTCTTAAAA GAACATGGCG CTTCAGAAAA GATACACCAG 
TCCACAAACC AGACTGCTGC CTTGAACTTG CTGTGGGAGA AGAGCTGCAG TGACAATGTG 
GTGGTCCGAA CAGCCTGCAG CGAGGGACTG GTGGCACTTG TTGCTCAGGG TCACGCAGAG 
TTCAGCTATG TTCTCAATGG GATACTCAAC CTGATTCCTT CTGCCAGGAA TATGTCCGGT 
TTGATAAAAG CCCTTATGAA ATTATTACAA ATGCAAGCAT CCAGGGAAGG ACAAGGTGGG 
GCAAAGGATC TTCAGGACCT ATATTCTATC AGAAATCCTC CTCAGCCTTT GATCACCGTG 
CTTGAGCACA GGCCTGATTG CTGGCCACTA CTCCTGCAGC AGCTGACAGC TTTTTTCCAG 
CAATGCCCTG AAAGGTCAGA AGCTTCATGT GTCCAAATAA TGGCACCATT TCTGTGGTAT 
CTCTACTGTG AACCATCCCA GTTACAAGAA TATGCTAAGC TTCGACTGTC CCTGCTCAAA 
GTCTTGCTTC AGCCCAGAGG TGTTTGTGAG GAAGCACAGC CATCAGTGTT GGAACAGCAG 
ATCCTTCAGC TGTGCTGTAA CATGGTTCCG TGCTTGCAGA TGAAAGACCT GATGCAGACA 
ACAGAGATGA CGCTGTTCGT GGAGGAAGTA TATCTAAGCC TTCTGCGCCA TCCTGCTTTC 
TGGAAGAGAC AGCTTACCCA GCTGACCGTT CAGTTGCTGT GCATCTGTGA AGTCAGCTTA 
AAGACAACTG GGGAATGCTC ATCTTTAATT CAGCTTTTAG AGCGCAGTGT TGAACTTCTT 
GGGGAGGATT TTCCTGTCAA ATTGGTCATT ATTGGAATAG CTTTAATACT TCTACAGACT 
CCAGCAAGTC AGCAGAAGCC AGTATTGAAC CTAACTCTGA AGCTCCTCTC GCTTACTGAG 
GGTCAGAGAA TCCCGAAGGC CTCTCTGCTG CTCCTGGTGC CTCTTCTGCA GATTCTGTCC 
TCCACTGCCT TGGAAGACTG TCTGGCCACG GGTGAGGAAG GCCCCTCCAG GCAGCAGTTA 
GCCCTCAGTC TGTTGGAAGT GCTACAGAGA GAGTGTCATG GCGACGACCG ACACACGATC 
TCCTGCAGGC TTGCTTTTCC TGTGAGCAGC ATGTATGGCT CAGTCTTCAC TGCCTGCAGG 
ATTCTTGAAG TCATGGGAGA AGCGTCCGCA GCGAGTGACT GGCTGGCTGC AGTAGAGTCC 
TTGCTCCCTG CCACCACAGT GATCCCCCCA CATGTTTTCT TACTGCTGGC TCACCTGCTT 
GTTGAAGATG GAGGACAGAA TCTTCAGCAG ATACTGAAGG TCACCACACA GTTAGCTCAA 
GCAGATTCCT CTCAGGTACC ACATCTGATT CCAGTTCTGA TGTTCAAATT GGGGAGACCC 
TTAGACCCTG TATCATATAA TCATATATTA TATACTTTGC CCACACTTGG TGTTCACAAG 
GTATGCGTAG GGCAGATTCT GCGGGTGATT CAACTGCTGG GGACCACACC ACGACTAAGA 
GCTGTCACGT TACGCCTGTT GACATCTCTG TGGGAAAAGC AGGATCGTGT GTACCCTGAA 
CTGCAGCGCT TCATGGCCAT GTCTGATGCA CCCTCTCTCT CTGTGGGCAA GGAGCTGCAG 
TGGGAGAAAC TGATTGCAAA AGCTGCATCC ATCAGAGACA TATGTAAACA GAGGCCCTAT 
CAGCATGGTG CAGACATGCT GGCAGCCATT TCTCAGGTGT TGAACGAATG CACCAAGCCT 
GATCAAGCTT CTCCAGCTGC TTTGGTGTTA CAAGGTCTTC ATGCACTCTG CCAGGCTGAG 
GTTGTCTGTA TTCGCTCCAC ATGGAATGCT CTCTCTCCCA AGTTGAGCTG TGACATGAGA 
CCACTTATAT TGAAGACCCT GAGTGAACTA TTCTCTCTGG TTCCTTCCTT AACAGTCAAT 
ACAGTTGAAT ATGAGAATTT TAAAGTTCAA GTCCTCACCT TCCTCTGGAC TCACACTCAA 
AACAAGGATC CCATTGTAGC AAGTGCCGCA TATAAATCTC TGTCTCACTT CAGTGCTGGA 
GAGCACACCA TTCTTCACCT GCCAGAAAAG ATAAGGCCAG AAATGCCGGT TCCCGATGAG 
TTGGATGAGG AGGAATCTGT GGACCTTTCC ATCCCTGGCT CTTGCTATCT CCGACTGCTG 
ACCATCACTG CTCCTTCAAT TTTACCAGCT TTGGAAGAAT TCTTCACGTC TCTTGTGAGA 
CAAGAGATGG TGAATATGCC CCGTGGGATA TACCACTCAG CATTGAAGGG AGGCGTCTGC 
TCAGACCAAG GGAAGACAGT AGCAGGAATC CCCAACTTTA TATTGAAGAC ATATGAAACA 
AACAAGCAAC CAGGACTGAA ACCCGGTCTG GCAGGTGGCA TGCTGTTTTG CTATGATGTG 
GCCATGTATC AGAGCAAAGA TGGTAAGCCA CTGAATCGGC TGATGGCCAG TAGAGGGCGA 
ACCTTCAAGC AGACAACACT GGCCCTTGTT CATGAAGTCC ACGTCCAGCT CTCAGAATGG 
CACCGTGCAA TCTTTCTTCC ACAGGCTTGG CTTGCATACA TGACTCGAGC TTACCATGCC 
ATTTTACAGG GACGGATAGC AGAGCTGGAG CTGCAGTTGC AACATGGCAA GGAGGGCCCC 
GAGGAGGTGC AGTACAAACG AAGCACGGCC TGGCTCTGGG TCAGAGACAT GCTGACAGAT 
GAGATCACCA AGACAGCTGC GAAAGAGAGT CCAGTGGTCA AAGGCAATGC TCTGTTGGCC 
TTAAGCAGCC TTGCCGTCGT GGTGTCCAAG CATGAAGCCA GCCTTTCCTC AGAGTCTGAT 
GGAGTCCTAG AGGTCCAACC TAATTTCCTT CCAGTGAAAG AGTGGGTTTC CATGGTGCTT 
AATACTGTCC TGGTCATTGT GGATAGCCAT TACCACCCCA GAGGTCAACT GTTCACCTGC 
TTTTATCATA AGTCCTATTC TGGCGAGAAC ACGGCAAGTG CTATTGCGCG CTCTGCCGCC 
GCCACGGCTT TGTCTCTCCT CGTGCCGGTT TTCATTATCT CTTGCAAAGA GAAGGTTGAG 
GAAATCCTGA ACATGCTGAC TGCCAGGTTA CCTGGGAAAC CAAGTGCTGA TGAATCTCAA 
GCCGTGCAAA TCCACATGGG CCTTGCCTTG GGGATGTTTC TCTCTCGCCT GTGTGAGGAG 
AAACTCAGTG ACATGTCTGG CCAACAGATG AGCCTTCTTC TGATGAAGTC CTTGGATGCC 
CTGGAGAGCT GCTGTTTCGA CCCCAGTCTT GAACACAACA CCGGCTGTAT ACTGGGAGTT 
GGACTTGCCC TGTCCCTCAT GAGCCACAGC AGCCACACAG AGTCACGCGT TCATGTGGCA 
GCATCCCTGC GGAAGCTGTC TGCCTACCTG GATGAGAGCG GGAGCCAGAG CAGGACATTT 
CAGGAGGTCC TTGCCTACAC ACTTAGCTGT GTGTGTACAT CAGCGTTCAG TGCTGGCATT 
ATTGAGGCTG TGGAGGCTGA AGAGACTATG ACCAAGCTCC AGCTGTTGGT GGAGAACAAC 
CAGCAGACTT CAGGCTTTGC ACTGGCCTTA GGAAACTTGG TTTATGGGTT GTCTGTGTGT 
GGACATGGAA AAGCTGAAGA CTTGGGCAAC AGACTTCGCC CCAGCTGGAT TAAAATTGTC 
CTGTCAGAGG GTGCCCCCAC CATGCTCTGT CTGGCAGCCC TTCATGGCCT GGTGGCACTG 
GTAGGCTCCG ATGTGGATGT CATGCAGTTC CAGTCAGAAG CCATCCACAA CTCCCAGTTT 
CAAGCAAGAC TTAATGAAGT CATTCGAACC TTAACTGAGG TCATCAGTGT CTCAGGGGTG 
ATTGGTCTTC AATCAAATGC AATATGGCTG CTAGGACATC TTCATCTATC CACCTTGTCT 
TCAAATCAAA GCAGAACTTC CGTTCCTACC GACTACAGCT ATTTGCCTGA AGGCAGTTTT 
ATTAGAGCAG CCATTGGCTT CTTCATCACA GGAGGAAAAA AAGGCCCTGA AGCTGTGCCA 
CCGTCCCTTC TCAAAGTAGT GATGAAACCC ATAGCAACTG TTGGGGAAAA CTACCAGTAC 
CCTCCTGCAA ACTTAGCCGC ACTCCTCTCT CCACTTATGA GGCTAAACTT TGGCGAGGAG 
ATACAGCAAC TGTGCCTTGA GATTGCTGTG TCCCAGGCAC CGTCGTCACA GAGTGCAGCT 
GCACTGCTGG GCTTGTGGGT GATGCCGCCG CTGGTCCACG GTCTGAGTCT GAATATCAAG 
AAATACCTCC TGGTATCTAT GCCTCTGTGG GCAAAGCATG TTTCTGATGA ACAGATCCAG 
GGTTTTGTTG AAAATCTGAT GGTGGCAGTT TTTAAACCGG CTTCCCAACC TTGTCATCCT 
GAGATGTGCC CAAGTGCCTT ACAGGGTCTG AGCCAGGCCA TGAAACTGCC CAGTCCTTCC 
CACCACCTCT GGAGTCTGCT CTGCGATGCT ACTGGGAGAA TTTTTGACCT TCTGCCAAAT 
AGGATTCGGA GAAATGATCT CGAGCTGTAC ATCAGTATAG CAAAGTGCCT CTCAGAGATG 
ACGGATGAAG GTGCCAATCA GGTTTCCCAG ATTACTAAGG AAAACATAGA AAAGGCTGCC 
TTTGTCAAGC TGTACTTAAT CTCTCAAGGA CGACTCCCGT TGATGAGCCT GACTGATTTA 
CTCACTGCTG CTATGCAACA CCCCTCGAAG GAGACTCTAG CCTGGATGAT CCTGCACAGC 
TTATACCAGG CACGCATTGT CAATCACACC AACACAGGTG TACTGAAGAG ACTGGAGTGG 
CTCCTGGAGC TGATGGGTTA TATGAGAAAC ATTGCTTACC AGTCGGCGTC TGCTCAGAAT 
GTGCCTCCTG CGGAGGCTTT GGACTTCTTG ATGCTGATAT TTGCTGCTGC AGTGGTAGCA 
TGGGCAGACC ATGAGGCCCC CCTTCTCCTT GGCCTCAGTG CCAGTTGGTT GCCATGGCAC 
CAGGAAAATG GCCCTGGTGG GCCAGCAGCA GCCTTACTTG GCAGGAGTCC GATGCACAGA 
GTCACTGTGC AGGAGGTTCT CACTCTCCTC CCCACGAGCA TGCTACTGCT CCTGCAAAAA 
GAGCCATGGA AGGAACAGAC CCAGAAGTTC ATTGACTGGC TGTTCAGCAT CATGGAAATT 
CCTAACGAAG CCTTTGCAGC CACGTCCAAG GATCTTTTGA AAGCCACCCT GCTGTCCTTG 
AGAGTTCTCC CAGAGTTTAA GAAGAAAGCT GTATGGACCC GAGCGTATGG CTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Focad ( XM_001053590.5 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_001053590.1 Rattus norvegicus focadhesin (Focad), transcript variant X2, mRNA.

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CloneID	ORa16728
Clone ID Related Accession (Same CDS sequence)	XM_017603022.1 , XM_001053590.5 , XM_233144.8 , XM_017603023.1
Accession Version	XM_001053590.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5397bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	focadhesin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000073.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_001053590.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGATG ATATCAAGAA AAGGTTTGAT TTTCCGAATT CCCTTATCCA GTCCCAGGCT 
GTAGGTCATC TTATTGCTGC TGTCTTAAAA GAACATGGCG CTTCAGAAAA GATACACCAG 
TCCACAAACC AGACTGCTGC CTTGAACTTG CTGTGGGAGA AGAGCTGCAG TGACAATGTG 
GTGGTCCGAA CAGCCTGCAG CGAGGGACTG GTGGCACTTG TTGCTCAGGG TCACGCAGAG 
TTCAGCTATG TTCTCAATGG GATACTCAAC CTGATTCCTT CTGCCAGGAA TATGTCCGGT 
TTGATAAAAG CCCTTATGAA ATTATTACAA ATGCAAGCAT CCAGGGAAGG ACAAGGTGGG 
GCAAAGGATC TTCAGGACCT ATATTCTATC AGAAATCCTC CTCAGCCTTT GATCACCGTG 
CTTGAGCACA GGCCTGATTG CTGGCCACTA CTCCTGCAGC AGCTGACAGC TTTTTTCCAG 
CAATGCCCTG AAAGGTCAGA AGCTTCATGT GTCCAAATAA TGGCACCATT TCTGTGGTAT 
CTCTACTGTG AACCATCCCA GTTACAAGAA TATGCTAAGC TTCGACTGTC CCTGCTCAAA 
GTCTTGCTTC AGCCCAGAGG TGTTTGTGAG GAAGCACAGC CATCAGTGTT GGAACAGCAG 
ATCCTTCAGC TGTGCTGTAA CATGGTTCCG TGCTTGCAGA TGAAAGACCT GATGCAGACA 
ACAGAGATGA CGCTGTTCGT GGAGGAAGTA TATCTAAGCC TTCTGCGCCA TCCTGCTTTC 
TGGAAGAGAC AGCTTACCCA GCTGACCGTT CAGTTGCTGT GCATCTGTGA AGTCAGCTTA 
AAGACAACTG GGGAATGCTC ATCTTTAATT CAGCTTTTAG AGCGCAGTGT TGAACTTCTT 
GGGGAGGATT TTCCTGTCAA ATTGGTCATT ATTGGAATAG CTTTAATACT TCTACAGACT 
CCAGCAAGTC AGCAGAAGCC AGTATTGAAC CTAACTCTGA AGCTCCTCTC GCTTACTGAG 
GGTCAGAGAA TCCCGAAGGC CTCTCTGCTG CTCCTGGTGC CTCTTCTGCA GATTCTGTCC 
TCCACTGCCT TGGAAGACTG TCTGGCCACG GGTGAGGAAG GCCCCTCCAG GCAGCAGTTA 
GCCCTCAGTC TGTTGGAAGT GCTACAGAGA GAGTGTCATG GCGACGACCG ACACACGATC 
TCCTGCAGGC TTGCTTTTCC TGTGAGCAGC ATGTATGGCT CAGTCTTCAC TGCCTGCAGG 
ATTCTTGAAG TCATGGGAGA AGCGTCCGCA GCGAGTGACT GGCTGGCTGC AGTAGAGTCC 
TTGCTCCCTG CCACCACAGT GATCCCCCCA CATGTTTTCT TACTGCTGGC TCACCTGCTT 
GTTGAAGATG GAGGACAGAA TCTTCAGCAG ATACTGAAGG TCACCACACA GTTAGCTCAA 
GCAGATTCCT CTCAGGTACC ACATCTGATT CCAGTTCTGA TGTTCAAATT GGGGAGACCC 
TTAGACCCTG TATCATATAA TCATATATTA TATACTTTGC CCACACTTGG TGTTCACAAG 
GTATGCGTAG GGCAGATTCT GCGGGTGATT CAACTGCTGG GGACCACACC ACGACTAAGA 
GCTGTCACGT TACGCCTGTT GACATCTCTG TGGGAAAAGC AGGATCGTGT GTACCCTGAA 
CTGCAGCGCT TCATGGCCAT GTCTGATGCA CCCTCTCTCT CTGTGGGCAA GGAGCTGCAG 
TGGGAGAAAC TGATTGCAAA AGCTGCATCC ATCAGAGACA TATGTAAACA GAGGCCCTAT 
CAGCATGGTG CAGACATGCT GGCAGCCATT TCTCAGGTGT TGAACGAATG CACCAAGCCT 
GATCAAGCTT CTCCAGCTGC TTTGGTGTTA CAAGGTCTTC ATGCACTCTG CCAGGCTGAG 
GTTGTCTGTA TTCGCTCCAC ATGGAATGCT CTCTCTCCCA AGTTGAGCTG TGACATGAGA 
CCACTTATAT TGAAGACCCT GAGTGAACTA TTCTCTCTGG TTCCTTCCTT AACAGTCAAT 
ACAGTTGAAT ATGAGAATTT TAAAGTTCAA GTCCTCACCT TCCTCTGGAC TCACACTCAA 
AACAAGGATC CCATTGTAGC AAGTGCCGCA TATAAATCTC TGTCTCACTT CAGTGCTGGA 
GAGCACACCA TTCTTCACCT GCCAGAAAAG ATAAGGCCAG AAATGCCGGT TCCCGATGAG 
TTGGATGAGG AGGAATCTGT GGACCTTTCC ATCCCTGGCT CTTGCTATCT CCGACTGCTG 
ACCATCACTG CTCCTTCAAT TTTACCAGCT TTGGAAGAAT TCTTCACGTC TCTTGTGAGA 
CAAGAGATGG TGAATATGCC CCGTGGGATA TACCACTCAG CATTGAAGGG AGGCGTCTGC 
TCAGACCAAG GGAAGACAGT AGCAGGAATC CCCAACTTTA TATTGAAGAC ATATGAAACA 
AACAAGCAAC CAGGACTGAA ACCCGGTCTG GCAGGTGGCA TGCTGTTTTG CTATGATGTG 
GCCATGTATC AGAGCAAAGA TGGTAAGCCA CTGAATCGGC TGATGGCCAG TAGAGGGCGA 
ACCTTCAAGC AGACAACACT GGCCCTTGTT CATGAAGTCC ACGTCCAGCT CTCAGAATGG 
CACCGTGCAA TCTTTCTTCC ACAGGCTTGG CTTGCATACA TGACTCGAGC TTACCATGCC 
ATTTTACAGG GACGGATAGC AGAGCTGGAG CTGCAGTTGC AACATGGCAA GGAGGGCCCC 
GAGGAGGTGC AGTACAAACG AAGCACGGCC TGGCTCTGGG TCAGAGACAT GCTGACAGAT 
GAGATCACCA AGACAGCTGC GAAAGAGAGT CCAGTGGTCA AAGGCAATGC TCTGTTGGCC 
TTAAGCAGCC TTGCCGTCGT GGTGTCCAAG CATGAAGCCA GCCTTTCCTC AGAGTCTGAT 
GGAGTCCTAG AGGTCCAACC TAATTTCCTT CCAGTGAAAG AGTGGGTTTC CATGGTGCTT 
AATACTGTCC TGGTCATTGT GGATAGCCAT TACCACCCCA GAGGTCAACT GTTCACCTGC 
TTTTATCATA AGTCCTATTC TGGCGAGAAC ACGGCAAGTG CTATTGCGCG CTCTGCCGCC 
GCCACGGCTT TGTCTCTCCT CGTGCCGGTT TTCATTATCT CTTGCAAAGA GAAGGTTGAG 
GAAATCCTGA ACATGCTGAC TGCCAGGTTA CCTGGGAAAC CAAGTGCTGA TGAATCTCAA 
GCCGTGCAAA TCCACATGGG CCTTGCCTTG GGGATGTTTC TCTCTCGCCT GTGTGAGGAG 
AAACTCAGTG ACATGTCTGG CCAACAGATG AGCCTTCTTC TGATGAAGTC CTTGGATGCC 
CTGGAGAGCT GCTGTTTCGA CCCCAGTCTT GAACACAACA CCGGCTGTAT ACTGGGAGTT 
GGACTTGCCC TGTCCCTCAT GAGCCACAGC AGCCACACAG AGTCACGCGT TCATGTGGCA 
GCATCCCTGC GGAAGCTGTC TGCCTACCTG GATGAGAGCG GGAGCCAGAG CAGGACATTT 
CAGGAGGTCC TTGCCTACAC ACTTAGCTGT GTGTGTACAT CAGCGTTCAG TGCTGGCATT 
ATTGAGGCTG TGGAGGCTGA AGAGACTATG ACCAAGCTCC AGCTGTTGGT GGAGAACAAC 
CAGCAGACTT CAGGCTTTGC ACTGGCCTTA GGAAACTTGG TTTATGGGTT GTCTGTGTGT 
GGACATGGAA AAGCTGAAGA CTTGGGCAAC AGACTTCGCC CCAGCTGGAT TAAAATTGTC 
CTGTCAGAGG GTGCCCCCAC CATGCTCTGT CTGGCAGCCC TTCATGGCCT GGTGGCACTG 
GTAGGCTCCG ATGTGGATGT CATGCAGTTC CAGTCAGAAG CCATCCACAA CTCCCAGTTT 
CAAGCAAGAC TTAATGAAGT CATTCGAACC TTAACTGAGG TCATCAGTGT CTCAGGGGTG 
ATTGGTCTTC AATCAAATGC AATATGGCTG CTAGGACATC TTCATCTATC CACCTTGTCT 
TCAAATCAAA GCAGAACTTC CGTTCCTACC GACTACAGCT ATTTGCCTGA AGGCAGTTTT 
ATTAGAGCAG CCATTGGCTT CTTCATCACA GGAGGAAAAA AAGGCCCTGA AGCTGTGCCA 
CCGTCCCTTC TCAAAGTAGT GATGAAACCC ATAGCAACTG TTGGGGAAAA CTACCAGTAC 
CCTCCTGCAA ACTTAGCCGC ACTCCTCTCT CCACTTATGA GGCTAAACTT TGGCGAGGAG 
ATACAGCAAC TGTGCCTTGA GATTGCTGTG TCCCAGGCAC CGTCGTCACA GAGTGCAGCT 
GCACTGCTGG GCTTGTGGGT GATGCCGCCG CTGGTCCACG GTCTGAGTCT GAATATCAAG 
AAATACCTCC TGGTATCTAT GCCTCTGTGG GCAAAGCATG TTTCTGATGA ACAGATCCAG 
GGTTTTGTTG AAAATCTGAT GGTGGCAGTT TTTAAACCGG CTTCCCAACC TTGTCATCCT 
GAGATGTGCC CAAGTGCCTT ACAGGGTCTG AGCCAGGCCA TGAAACTGCC CAGTCCTTCC 
CACCACCTCT GGAGTCTGCT CTGCGATGCT ACTGGGAGAA TTTTTGACCT TCTGCCAAAT 
AGGATTCGGA GAAATGATCT CGAGCTGTAC ATCAGTATAG CAAAGTGCCT CTCAGAGATG 
ACGGATGAAG GTGCCAATCA GGTTTCCCAG ATTACTAAGG AAAACATAGA AAAGGCTGCC 
TTTGTCAAGC TGTACTTAAT CTCTCAAGGA CGACTCCCGT TGATGAGCCT GACTGATTTA 
CTCACTGCTG CTATGCAACA CCCCTCGAAG GAGACTCTAG CCTGGATGAT CCTGCACAGC 
TTATACCAGG CACGCATTGT CAATCACACC AACACAGGTG TACTGAAGAG ACTGGAGTGG 
CTCCTGGAGC TGATGGGTTA TATGAGAAAC ATTGCTTACC AGTCGGCGTC TGCTCAGAAT 
GTGCCTCCTG CGGAGGCTTT GGACTTCTTG ATGCTGATAT TTGCTGCTGC AGTGGTAGCA 
TGGGCAGACC ATGAGGCCCC CCTTCTCCTT GGCCTCAGTG CCAGTTGGTT GCCATGGCAC 
CAGGAAAATG GCCCTGGTGG GCCAGCAGCA GCCTTACTTG GCAGGAGTCC GATGCACAGA 
GTCACTGTGC AGGAGGTTCT CACTCTCCTC CCCACGAGCA TGCTACTGCT CCTGCAAAAA 
GAGCCATGGA AGGAACAGAC CCAGAAGTTC ATTGACTGGC TGTTCAGCAT CATGGAAATT 
CCTAACGAAG CCTTTGCAGC CACGTCCAAG GATCTTTTGA AAGCCACCCT GCTGTCCTTG 
AGAGTTCTCC CAGAGTTTAA GAAGAAAGCT GTATGGACCC GAGCGTATGG CTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001053590.1
CDS	133..5529
Translation	MSDDIKKRFDFPNSLIQSQAVGHLIAAVLKEHGASEKIHQSTNQTAALNLLWEKSCSDNVVVRTACSEGLVALVAQGHAEFSYVLNGILNLIPSARNMSGLIKALMKLLQMQASREGQGGAKDLQDLYSIRNPPQPLITVLEHRPDCWPLLLQQLTAFFQQCPERSEASCVQIMAPFLWYLYCEPSQLQEYAKLRLSLLKVLLQPRGVCEEAQPSVLEQQILQLCCNMVPCLQMKDLMQTTEMTLFVEEVYLSLLRHPAFWKRQLTQLTVQLLCICEVSLKTTGECSSLIQLLERSVELLGEDFPVKLVIIGIALILLQTPASQQKPVLNLTLKLLSLTEGQRIPKASLLLLVPLLQILSSTALEDCLATGEEGPSRQQLALSLLEVLQRECHGDDRHTISCRLAFPVSSMYGSVFTACRILEVMGEASAASDWLAAVESLLPATTVIPPHVFLLLAHLLVEDGGQNLQQILKVTTQLAQADSSQVPHLIPVLMFKLGRPLDPVSYNHILYTLPTLGVHKVCVGQILRVIQLLGTTPRLRAVTLRLLTSLWEKQDRVYPELQRFMAMSDAPSLSVGKELQWEKLIAKAASIRDICKQRPYQHGADMLAAISQVLNECTKPDQASPAALVLQGLHALCQAEVVCIRSTWNALSPKLSCDMRPLILKTLSELFSLVPSLTVNTVEYENFKVQVLTFLWTHTQNKDPIVASAAYKSLSHFSAGEHTILHLPEKIRPEMPVPDELDEEESVDLSIPGSCYLRLLTITAPSILPALEEFFTSLVRQEMVNMPRGIYHSALKGGVCSDQGKTVAGIPNFILKTYETNKQPGLKPGLAGGMLFCYDVAMYQSKDGKPLNRLMASRGRTFKQTTLALVHEVHVQLSEWHRAIFLPQAWLAYMTRAYHAILQGRIAELELQLQHGKEGPEEVQYKRSTAWLWVRDMLTDEITKTAAKESPVVKGNALLALSSLAVVVSKHEASLSSESDGVLEVQPNFLPVKEWVSMVLNTVLVIVDSHYHPRGQLFTCFYHKSYSGENTASAIARSAAATALSLLVPVFIISCKEKVEEILNMLTARLPGKPSADESQAVQIHMGLALGMFLSRLCEEKLSDMSGQQMSLLLMKSLDALESCCFDPSLEHNTGCILGVGLALSLMSHSSHTESRVHVAASLRKLSAYLDESGSQSRTFQEVLAYTLSCVCTSAFSAGIIEAVEAEETMTKLQLLVENNQQTSGFALALGNLVYGLSVCGHGKAEDLGNRLRPSWIKIVLSEGAPTMLCLAALHGLVALVGSDVDVMQFQSEAIHNSQFQARLNEVIRTLTEVISVSGVIGLQSNAIWLLGHLHLSTLSSNQSRTSVPTDYSYLPEGSFIRAAIGFFITGGKKGPEAVPPSLLKVVMKPIATVGENYQYPPANLAALLSPLMRLNFGEEIQQLCLEIAVSQAPSSQSAAALLGLWVMPPLVHGLSLNIKKYLLVSMPLWAKHVSDEQIQGFVENLMVAVFKPASQPCHPEMCPSALQGLSQAMKLPSPSHHLWSLLCDATGRIFDLLPNRIRRNDLELYISIAKCLSEMTDEGANQVSQITKENIEKAAFVKLYLISQGRLPLMSLTDLLTAAMQHPSKETLAWMILHSLYQARIVNHTNTGVLKRLEWLLELMGYMRNIAYQSASAQNVPPAEALDFLMLIFAAAVVAWADHEAPLLLGLSASWLPWHQENGPGGPAAALLGRSPMHRVTVQEVLTLLPTSMLLLLQKEPWKEQTQKFIDWLFSIMEIPNEAFAATSKDLLKATLLSLRVLPEFKKKAVWTRAYGW

Target ORF information:

RefSeq Version	XM_001053590.5
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus focadhesin (Focad), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001053590.5

ORF Insert Sequence:

ATGTCAGATG ATATCAAGAA AAGGTTTGAT TTTCCGAATT CCCTTATCCA GTCCCAGGCT 
GTAGGTCATC TTATTGCTGC TGTCTTAAAA GAACATGGCG CTTCAGAAAA GATACACCAG 
TCCACAAACC AGACTGCTGC CTTGAACTTG CTGTGGGAGA AGAGCTGCAG TGACAATGTG 
GTGGTCCGAA CAGCCTGCAG CGAGGGACTG GTGGCACTTG TTGCTCAGGG TCACGCAGAG 
TTCAGCTATG TTCTCAATGG GATACTCAAC CTGATTCCTT CTGCCAGGAA TATGTCCGGT 
TTGATAAAAG CCCTTATGAA ATTATTACAA ATGCAAGCAT CCAGGGAAGG ACAAGGTGGG 
GCAAAGGATC TTCAGGACCT ATATTCTATC AGAAATCCTC CTCAGCCTTT GATCACCGTG 
CTTGAGCACA GGCCTGATTG CTGGCCACTA CTCCTGCAGC AGCTGACAGC TTTTTTCCAG 
CAATGCCCTG AAAGGTCAGA AGCTTCATGT GTCCAAATAA TGGCACCATT TCTGTGGTAT 
CTCTACTGTG AACCATCCCA GTTACAAGAA TATGCTAAGC TTCGACTGTC CCTGCTCAAA 
GTCTTGCTTC AGCCCAGAGG TGTTTGTGAG GAAGCACAGC CATCAGTGTT GGAACAGCAG 
ATCCTTCAGC TGTGCTGTAA CATGGTTCCG TGCTTGCAGA TGAAAGACCT GATGCAGACA 
ACAGAGATGA CGCTGTTCGT GGAGGAAGTA TATCTAAGCC TTCTGCGCCA TCCTGCTTTC 
TGGAAGAGAC AGCTTACCCA GCTGACCGTT CAGTTGCTGT GCATCTGTGA AGTCAGCTTA 
AAGACAACTG GGGAATGCTC ATCTTTAATT CAGCTTTTAG AGCGCAGTGT TGAACTTCTT 
GGGGAGGATT TTCCTGTCAA ATTGGTCATT ATTGGAATAG CTTTAATACT TCTACAGACT 
CCAGCAAGTC AGCAGAAGCC AGTATTGAAC CTAACTCTGA AGCTCCTCTC GCTTACTGAG 
GGTCAGAGAA TCCCGAAGGC CTCTCTGCTG CTCCTGGTGC CTCTTCTGCA GATTCTGTCC 
TCCACTGCCT TGGAAGACTG TCTGGCCACG GGTGAGGAAG GCCCCTCCAG GCAGCAGTTA 
GCCCTCAGTC TGTTGGAAGT GCTACAGAGA GAGTGTCATG GCGACGACCG ACACACGATC 
TCCTGCAGGC TTGCTTTTCC TGTGAGCAGC ATGTATGGCT CAGTCTTCAC TGCCTGCAGG 
ATTCTTGAAG TCATGGGAGA AGCGTCCGCA GCGAGTGACT GGCTGGCTGC AGTAGAGTCC 
TTGCTCCCTG CCACCACAGT GATCCCCCCA CATGTTTTCT TACTGCTGGC TCACCTGCTT 
GTTGAAGATG GAGGACAGAA TCTTCAGCAG ATACTGAAGG TCACCACACA GTTAGCTCAA 
GCAGATTCCT CTCAGGTACC ACATCTGATT CCAGTTCTGA TGTTCAAATT GGGGAGACCC 
TTAGACCCTG TATCATATAA TCATATATTA TATACTTTGC CCACACTTGG TGTTCACAAG 
GTATGCGTAG GGCAGATTCT GCGGGTGATT CAACTGCTGG GGACCACACC ACGACTAAGA 
GCTGTCACGT TACGCCTGTT GACATCTCTG TGGGAAAAGC AGGATCGTGT GTACCCTGAA 
CTGCAGCGCT TCATGGCCAT GTCTGATGCA CCCTCTCTCT CTGTGGGCAA GGAGCTGCAG 
TGGGAGAAAC TGATTGCAAA AGCTGCATCC ATCAGAGACA TATGTAAACA GAGGCCCTAT 
CAGCATGGTG CAGACATGCT GGCAGCCATT TCTCAGGTGT TGAACGAATG CACCAAGCCT 
GATCAAGCTT CTCCAGCTGC TTTGGTGTTA CAAGGTCTTC ATGCACTCTG CCAGGCTGAG 
GTTGTCTGTA TTCGCTCCAC ATGGAATGCT CTCTCTCCCA AGTTGAGCTG TGACATGAGA 
CCACTTATAT TGAAGACCCT GAGTGAACTA TTCTCTCTGG TTCCTTCCTT AACAGTCAAT 
ACAGTTGAAT ATGAGAATTT TAAAGTTCAA GTCCTCACCT TCCTCTGGAC TCACACTCAA 
AACAAGGATC CCATTGTAGC AAGTGCCGCA TATAAATCTC TGTCTCACTT CAGTGCTGGA 
GAGCACACCA TTCTTCACCT GCCAGAAAAG ATAAGGCCAG AAATGCCGGT TCCCGATGAG 
TTGGATGAGG AGGAATCTGT GGACCTTTCC ATCCCTGGCT CTTGCTATCT CCGACTGCTG 
ACCATCACTG CTCCTTCAAT TTTACCAGCT TTGGAAGAAT TCTTCACGTC TCTTGTGAGA 
CAAGAGATGG TGAATATGCC CCGTGGGATA TACCACTCAG CATTGAAGGG AGGCGTCTGC 
TCAGACCAAG GGAAGACAGT AGCAGGAATC CCCAACTTTA TATTGAAGAC ATATGAAACA 
AACAAGCAAC CAGGACTGAA ACCCGGTCTG GCAGGTGGCA TGCTGTTTTG CTATGATGTG 
GCCATGTATC AGAGCAAAGA TGGTAAGCCA CTGAATCGGC TGATGGCCAG TAGAGGGCGA 
ACCTTCAAGC AGACAACACT GGCCCTTGTT CATGAAGTCC ACGTCCAGCT CTCAGAATGG 
CACCGTGCAA TCTTTCTTCC ACAGGCTTGG CTTGCATACA TGACTCGAGC TTACCATGCC 
ATTTTACAGG GACGGATAGC AGAGCTGGAG CTGCAGTTGC AACATGGCAA GGAGGGCCCC 
GAGGAGGTGC AGTACAAACG AAGCACGGCC TGGCTCTGGG TCAGAGACAT GCTGACAGAT 
GAGATCACCA AGACAGCTGC GAAAGAGAGT CCAGTGGTCA AAGGCAATGC TCTGTTGGCC 
TTAAGCAGCC TTGCCGTCGT GGTGTCCAAG CATGAAGCCA GCCTTTCCTC AGAGTCTGAT 
GGAGTCCTAG AGGTCCAACC TAATTTCCTT CCAGTGAAAG AGTGGGTTTC CATGGTGCTT 
AATACTGTCC TGGTCATTGT GGATAGCCAT TACCACCCCA GAGGTCAACT GTTCACCTGC 
TTTTATCATA AGTCCTATTC TGGCGAGAAC ACGGCAAGTG CTATTGCGCG CTCTGCCGCC 
GCCACGGCTT TGTCTCTCCT CGTGCCGGTT TTCATTATCT CTTGCAAAGA GAAGGTTGAG 
GAAATCCTGA ACATGCTGAC TGCCAGGTTA CCTGGGAAAC CAAGTGCTGA TGAATCTCAA 
GCCGTGCAAA TCCACATGGG CCTTGCCTTG GGGATGTTTC TCTCTCGCCT GTGTGAGGAG 
AAACTCAGTG ACATGTCTGG CCAACAGATG AGCCTTCTTC TGATGAAGTC CTTGGATGCC 
CTGGAGAGCT GCTGTTTCGA CCCCAGTCTT GAACACAACA CCGGCTGTAT ACTGGGAGTT 
GGACTTGCCC TGTCCCTCAT GAGCCACAGC AGCCACACAG AGTCACGCGT TCATGTGGCA 
GCATCCCTGC GGAAGCTGTC TGCCTACCTG GATGAGAGCG GGAGCCAGAG CAGGACATTT 
CAGGAGGTCC TTGCCTACAC ACTTAGCTGT GTGTGTACAT CAGCGTTCAG TGCTGGCATT 
ATTGAGGCTG TGGAGGCTGA AGAGACTATG ACCAAGCTCC AGCTGTTGGT GGAGAACAAC 
CAGCAGACTT CAGGCTTTGC ACTGGCCTTA GGAAACTTGG TTTATGGGTT GTCTGTGTGT 
GGACATGGAA AAGCTGAAGA CTTGGGCAAC AGACTTCGCC CCAGCTGGAT TAAAATTGTC 
CTGTCAGAGG GTGCCCCCAC CATGCTCTGT CTGGCAGCCC TTCATGGCCT GGTGGCACTG 
GTAGGCTCCG ATGTGGATGT CATGCAGTTC CAGTCAGAAG CCATCCACAA CTCCCAGTTT 
CAAGCAAGAC TTAATGAAGT CATTCGAACC TTAACTGAGG TCATCAGTGT CTCAGGGGTG 
ATTGGTCTTC AATCAAATGC AATATGGCTG CTAGGACATC TTCATCTATC CACCTTGTCT 
TCAAATCAAA GCAGAACTTC CGTTCCTACC GACTACAGCT ATTTGCCTGA AGGCAGTTTT 
ATTAGAGCAG CCATTGGCTT CTTCATCACA GGAGGAAAAA AAGGCCCTGA AGCTGTGCCA 
CCGTCCCTTC TCAAAGTAGT GATGAAACCC ATAGCAACTG TTGGGGAAAA CTACCAGTAC 
CCTCCTGCAA ACTTAGCCGC ACTCCTCTCT CCACTTATGA GGCTAAACTT TGGCGAGGAG 
ATACAGCAAC TGTGCCTTGA GATTGCTGTG TCCCAGGCAC CGTCGTCACA GAGTGCAGCT 
GCACTGCTGG GCTTGTGGGT GATGCCGCCG CTGGTCCACG GTCTGAGTCT GAATATCAAG 
AAATACCTCC TGGTATCTAT GCCTCTGTGG GCAAAGCATG TTTCTGATGA ACAGATCCAG 
GGTTTTGTTG AAAATCTGAT GGTGGCAGTT TTTAAACCGG CTTCCCAACC TTGTCATCCT 
GAGATGTGCC CAAGTGCCTT ACAGGGTCTG AGCCAGGCCA TGAAACTGCC CAGTCCTTCC 
CACCACCTCT GGAGTCTGCT CTGCGATGCT ACTGGGAGAA TTTTTGACCT TCTGCCAAAT 
AGGATTCGGA GAAATGATCT CGAGCTGTAC ATCAGTATAG CAAAGTGCCT CTCAGAGATG 
ACGGATGAAG GTGCCAATCA GGTTTCCCAG ATTACTAAGG AAAACATAGA AAAGGCTGCC 
TTTGTCAAGC TGTACTTAAT CTCTCAAGGA CGACTCCCGT TGATGAGCCT GACTGATTTA 
CTCACTGCTG CTATGCAACA CCCCTCGAAG GAGACTCTAG CCTGGATGAT CCTGCACAGC 
TTATACCAGG CACGCATTGT CAATCACACC AACACAGGTG TACTGAAGAG ACTGGAGTGG 
CTCCTGGAGC TGATGGGTTA TATGAGAAAC ATTGCTTACC AGTCGGCGTC TGCTCAGAAT 
GTGCCTCCTG CGGAGGCTTT GGACTTCTTG ATGCTGATAT TTGCTGCTGC AGTGGTAGCA 
TGGGCAGACC ATGAGGCCCC CCTTCTCCTT GGCCTCAGTG CCAGTTGGTT GCCATGGCAC 
CAGGAAAATG GCCCTGGTGG GCCAGCAGCA GCCTTACTTG GCAGGAGTCC GATGCACAGA 
GTCACTGTGC AGGAGGTTCT CACTCTCCTC CCCACGAGCA TGCTACTGCT CCTGCAAAAA 
GAGCCATGGA AGGAACAGAC CCAGAAGTTC ATTGACTGGC TGTTCAGCAT CATGGAAATT 
CCTAACGAAG CCTTTGCAGC CACGTCCAAG GATCTTTTGA AAGCCACCCT GCTGTCCTTG 
AGAGTTCTCC CAGAGTTTAA GAAGAAAGCT GTATGGACCC GAGCGTATGG CTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Focad ( XM_233144.8 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_233144.5 Rattus norvegicus focadhesin (Focad), transcript variant X2, mRNA.

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CloneID	ORa16728
Clone ID Related Accession (Same CDS sequence)	XM_017603022.1 , XM_001053590.5 , XM_233144.8 , XM_017603023.1
Accession Version	XM_233144.8 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5397bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	focadhesin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_233144.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGATG ATATCAAGAA AAGGTTTGAT TTTCCGAATT CCCTTATCCA GTCCCAGGCT 
GTAGGTCATC TTATTGCTGC TGTCTTAAAA GAACATGGCG CTTCAGAAAA GATACACCAG 
TCCACAAACC AGACTGCTGC CTTGAACTTG CTGTGGGAGA AGAGCTGCAG TGACAATGTG 
GTGGTCCGAA CAGCCTGCAG CGAGGGACTG GTGGCACTTG TTGCTCAGGG TCACGCAGAG 
TTCAGCTATG TTCTCAATGG GATACTCAAC CTGATTCCTT CTGCCAGGAA TATGTCCGGT 
TTGATAAAAG CCCTTATGAA ATTATTACAA ATGCAAGCAT CCAGGGAAGG ACAAGGTGGG 
GCAAAGGATC TTCAGGACCT ATATTCTATC AGAAATCCTC CTCAGCCTTT GATCACCGTG 
CTTGAGCACA GGCCTGATTG CTGGCCACTA CTCCTGCAGC AGCTGACAGC TTTTTTCCAG 
CAATGCCCTG AAAGGTCAGA AGCTTCATGT GTCCAAATAA TGGCACCATT TCTGTGGTAT 
CTCTACTGTG AACCATCCCA GTTACAAGAA TATGCTAAGC TTCGACTGTC CCTGCTCAAA 
GTCTTGCTTC AGCCCAGAGG TGTTTGTGAG GAAGCACAGC CATCAGTGTT GGAACAGCAG 
ATCCTTCAGC TGTGCTGTAA CATGGTTCCG TGCTTGCAGA TGAAAGACCT GATGCAGACA 
ACAGAGATGA CGCTGTTCGT GGAGGAAGTA TATCTAAGCC TTCTGCGCCA TCCTGCTTTC 
TGGAAGAGAC AGCTTACCCA GCTGACCGTT CAGTTGCTGT GCATCTGTGA AGTCAGCTTA 
AAGACAACTG GGGAATGCTC ATCTTTAATT CAGCTTTTAG AGCGCAGTGT TGAACTTCTT 
GGGGAGGATT TTCCTGTCAA ATTGGTCATT ATTGGAATAG CTTTAATACT TCTACAGACT 
CCAGCAAGTC AGCAGAAGCC AGTATTGAAC CTAACTCTGA AGCTCCTCTC GCTTACTGAG 
GGTCAGAGAA TCCCGAAGGC CTCTCTGCTG CTCCTGGTGC CTCTTCTGCA GATTCTGTCC 
TCCACTGCCT TGGAAGACTG TCTGGCCACG GGTGAGGAAG GCCCCTCCAG GCAGCAGTTA 
GCCCTCAGTC TGTTGGAAGT GCTACAGAGA GAGTGTCATG GCGACGACCG ACACACGATC 
TCCTGCAGGC TTGCTTTTCC TGTGAGCAGC ATGTATGGCT CAGTCTTCAC TGCCTGCAGG 
ATTCTTGAAG TCATGGGAGA AGCGTCCGCA GCGAGTGACT GGCTGGCTGC AGTAGAGTCC 
TTGCTCCCTG CCACCACAGT GATCCCCCCA CATGTTTTCT TACTGCTGGC TCACCTGCTT 
GTTGAAGATG GAGGACAGAA TCTTCAGCAG ATACTGAAGG TCACCACACA GTTAGCTCAA 
GCAGATTCCT CTCAGGTACC ACATCTGATT CCAGTTCTGA TGTTCAAATT GGGGAGACCC 
TTAGACCCTG TATCATATAA TCATATATTA TATACTTTGC CCACACTTGG TGTTCACAAG 
GTATGCGTAG GGCAGATTCT GCGGGTGATT CAACTGCTGG GGACCACACC ACGACTAAGA 
GCTGTCACGT TACGCCTGTT GACATCTCTG TGGGAAAAGC AGGATCGTGT GTACCCTGAA 
CTGCAGCGCT TCATGGCCAT GTCTGATGCA CCCTCTCTCT CTGTGGGCAA GGAGCTGCAG 
TGGGAGAAAC TGATTGCAAA AGCTGCATCC ATCAGAGACA TATGTAAACA GAGGCCCTAT 
CAGCATGGTG CAGACATGCT GGCAGCCATT TCTCAGGTGT TGAACGAATG CACCAAGCCT 
GATCAAGCTT CTCCAGCTGC TTTGGTGTTA CAAGGTCTTC ATGCACTCTG CCAGGCTGAG 
GTTGTCTGTA TTCGCTCCAC ATGGAATGCT CTCTCTCCCA AGTTGAGCTG TGACATGAGA 
CCACTTATAT TGAAGACCCT GAGTGAACTA TTCTCTCTGG TTCCTTCCTT AACAGTCAAT 
ACAGTTGAAT ATGAGAATTT TAAAGTTCAA GTCCTCACCT TCCTCTGGAC TCACACTCAA 
AACAAGGATC CCATTGTAGC AAGTGCCGCA TATAAATCTC TGTCTCACTT CAGTGCTGGA 
GAGCACACCA TTCTTCACCT GCCAGAAAAG ATAAGGCCAG AAATGCCGGT TCCCGATGAG 
TTGGATGAGG AGGAATCTGT GGACCTTTCC ATCCCTGGCT CTTGCTATCT CCGACTGCTG 
ACCATCACTG CTCCTTCAAT TTTACCAGCT TTGGAAGAAT TCTTCACGTC TCTTGTGAGA 
CAAGAGATGG TGAATATGCC CCGTGGGATA TACCACTCAG CATTGAAGGG AGGCGTCTGC 
TCAGACCAAG GGAAGACAGT AGCAGGAATC CCCAACTTTA TATTGAAGAC ATATGAAACA 
AACAAGCAAC CAGGACTGAA ACCCGGTCTG GCAGGTGGCA TGCTGTTTTG CTATGATGTG 
GCCATGTATC AGAGCAAAGA TGGTAAGCCA CTGAATCGGC TGATGGCCAG TAGAGGGCGA 
ACCTTCAAGC AGACAACACT GGCCCTTGTT CATGAAGTCC ACGTCCAGCT CTCAGAATGG 
CACCGTGCAA TCTTTCTTCC ACAGGCTTGG CTTGCATACA TGACTCGAGC TTACCATGCC 
ATTTTACAGG GACGGATAGC AGAGCTGGAG CTGCAGTTGC AACATGGCAA GGAGGGCCCC 
GAGGAGGTGC AGTACAAACG AAGCACGGCC TGGCTCTGGG TCAGAGACAT GCTGACAGAT 
GAGATCACCA AGACAGCTGC GAAAGAGAGT CCAGTGGTCA AAGGCAATGC TCTGTTGGCC 
TTAAGCAGCC TTGCCGTCGT GGTGTCCAAG CATGAAGCCA GCCTTTCCTC AGAGTCTGAT 
GGAGTCCTAG AGGTCCAACC TAATTTCCTT CCAGTGAAAG AGTGGGTTTC CATGGTGCTT 
AATACTGTCC TGGTCATTGT GGATAGCCAT TACCACCCCA GAGGTCAACT GTTCACCTGC 
TTTTATCATA AGTCCTATTC TGGCGAGAAC ACGGCAAGTG CTATTGCGCG CTCTGCCGCC 
GCCACGGCTT TGTCTCTCCT CGTGCCGGTT TTCATTATCT CTTGCAAAGA GAAGGTTGAG 
GAAATCCTGA ACATGCTGAC TGCCAGGTTA CCTGGGAAAC CAAGTGCTGA TGAATCTCAA 
GCCGTGCAAA TCCACATGGG CCTTGCCTTG GGGATGTTTC TCTCTCGCCT GTGTGAGGAG 
AAACTCAGTG ACATGTCTGG CCAACAGATG AGCCTTCTTC TGATGAAGTC CTTGGATGCC 
CTGGAGAGCT GCTGTTTCGA CCCCAGTCTT GAACACAACA CCGGCTGTAT ACTGGGAGTT 
GGACTTGCCC TGTCCCTCAT GAGCCACAGC AGCCACACAG AGTCACGCGT TCATGTGGCA 
GCATCCCTGC GGAAGCTGTC TGCCTACCTG GATGAGAGCG GGAGCCAGAG CAGGACATTT 
CAGGAGGTCC TTGCCTACAC ACTTAGCTGT GTGTGTACAT CAGCGTTCAG TGCTGGCATT 
ATTGAGGCTG TGGAGGCTGA AGAGACTATG ACCAAGCTCC AGCTGTTGGT GGAGAACAAC 
CAGCAGACTT CAGGCTTTGC ACTGGCCTTA GGAAACTTGG TTTATGGGTT GTCTGTGTGT 
GGACATGGAA AAGCTGAAGA CTTGGGCAAC AGACTTCGCC CCAGCTGGAT TAAAATTGTC 
CTGTCAGAGG GTGCCCCCAC CATGCTCTGT CTGGCAGCCC TTCATGGCCT GGTGGCACTG 
GTAGGCTCCG ATGTGGATGT CATGCAGTTC CAGTCAGAAG CCATCCACAA CTCCCAGTTT 
CAAGCAAGAC TTAATGAAGT CATTCGAACC TTAACTGAGG TCATCAGTGT CTCAGGGGTG 
ATTGGTCTTC AATCAAATGC AATATGGCTG CTAGGACATC TTCATCTATC CACCTTGTCT 
TCAAATCAAA GCAGAACTTC CGTTCCTACC GACTACAGCT ATTTGCCTGA AGGCAGTTTT 
ATTAGAGCAG CCATTGGCTT CTTCATCACA GGAGGAAAAA AAGGCCCTGA AGCTGTGCCA 
CCGTCCCTTC TCAAAGTAGT GATGAAACCC ATAGCAACTG TTGGGGAAAA CTACCAGTAC 
CCTCCTGCAA ACTTAGCCGC ACTCCTCTCT CCACTTATGA GGCTAAACTT TGGCGAGGAG 
ATACAGCAAC TGTGCCTTGA GATTGCTGTG TCCCAGGCAC CGTCGTCACA GAGTGCAGCT 
GCACTGCTGG GCTTGTGGGT GATGCCGCCG CTGGTCCACG GTCTGAGTCT GAATATCAAG 
AAATACCTCC TGGTATCTAT GCCTCTGTGG GCAAAGCATG TTTCTGATGA ACAGATCCAG 
GGTTTTGTTG AAAATCTGAT GGTGGCAGTT TTTAAACCGG CTTCCCAACC TTGTCATCCT 
GAGATGTGCC CAAGTGCCTT ACAGGGTCTG AGCCAGGCCA TGAAACTGCC CAGTCCTTCC 
CACCACCTCT GGAGTCTGCT CTGCGATGCT ACTGGGAGAA TTTTTGACCT TCTGCCAAAT 
AGGATTCGGA GAAATGATCT CGAGCTGTAC ATCAGTATAG CAAAGTGCCT CTCAGAGATG 
ACGGATGAAG GTGCCAATCA GGTTTCCCAG ATTACTAAGG AAAACATAGA AAAGGCTGCC 
TTTGTCAAGC TGTACTTAAT CTCTCAAGGA CGACTCCCGT TGATGAGCCT GACTGATTTA 
CTCACTGCTG CTATGCAACA CCCCTCGAAG GAGACTCTAG CCTGGATGAT CCTGCACAGC 
TTATACCAGG CACGCATTGT CAATCACACC AACACAGGTG TACTGAAGAG ACTGGAGTGG 
CTCCTGGAGC TGATGGGTTA TATGAGAAAC ATTGCTTACC AGTCGGCGTC TGCTCAGAAT 
GTGCCTCCTG CGGAGGCTTT GGACTTCTTG ATGCTGATAT TTGCTGCTGC AGTGGTAGCA 
TGGGCAGACC ATGAGGCCCC CCTTCTCCTT GGCCTCAGTG CCAGTTGGTT GCCATGGCAC 
CAGGAAAATG GCCCTGGTGG GCCAGCAGCA GCCTTACTTG GCAGGAGTCC GATGCACAGA 
GTCACTGTGC AGGAGGTTCT CACTCTCCTC CCCACGAGCA TGCTACTGCT CCTGCAAAAA 
GAGCCATGGA AGGAACAGAC CCAGAAGTTC ATTGACTGGC TGTTCAGCAT CATGGAAATT 
CCTAACGAAG CCTTTGCAGC CACGTCCAAG GATCTTTTGA AAGCCACCCT GCTGTCCTTG 
AGAGTTCTCC CAGAGTTTAA GAAGAAAGCT GTATGGACCC GAGCGTATGG CTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_233144.5
CDS	133..5529
Translation	MSDDIKKRFDFPNSLIQSQAVGHLIAAVLKEHGASEKIHQSTNQTAALNLLWEKSCSDNVVVRTACSEGLVALVAQGHAEFSYVLNGILNLIPSARNMSGLIKALMKLLQMQASREGQGGAKDLQDLYSIRNPPQPLITVLEHRPDCWPLLLQQLTAFFQQCPERSEASCVQIMAPFLWYLYCEPSQLQEYAKLRLSLLKVLLQPRGVCEEAQPSVLEQQILQLCCNMVPCLQMKDLMQTTEMTLFVEEVYLSLLRHPAFWKRQLTQLTVQLLCICEVSLKTTGECSSLIQLLERSVELLGEDFPVKLVIIGIALILLQTPASQQKPVLNLTLKLLSLTEGQRIPKASLLLLVPLLQILSSTALEDCLATGEEGPSRQQLALSLLEVLQRECHGDDRHTISCRLAFPVSSMYGSVFTACRILEVMGEASAASDWLAAVESLLPATTVIPPHVFLLLAHLLVEDGGQNLQQILKVTTQLAQADSSQVPHLIPVLMFKLGRPLDPVSYNHILYTLPTLGVHKVCVGQILRVIQLLGTTPRLRAVTLRLLTSLWEKQDRVYPELQRFMAMSDAPSLSVGKELQWEKLIAKAASIRDICKQRPYQHGADMLAAISQVLNECTKPDQASPAALVLQGLHALCQAEVVCIRSTWNALSPKLSCDMRPLILKTLSELFSLVPSLTVNTVEYENFKVQVLTFLWTHTQNKDPIVASAAYKSLSHFSAGEHTILHLPEKIRPEMPVPDELDEEESVDLSIPGSCYLRLLTITAPSILPALEEFFTSLVRQEMVNMPRGIYHSALKGGVCSDQGKTVAGIPNFILKTYETNKQPGLKPGLAGGMLFCYDVAMYQSKDGKPLNRLMASRGRTFKQTTLALVHEVHVQLSEWHRAIFLPQAWLAYMTRAYHAILQGRIAELELQLQHGKEGPEEVQYKRSTAWLWVRDMLTDEITKTAAKESPVVKGNALLALSSLAVVVSKHEASLSSESDGVLEVQPNFLPVKEWVSMVLNTVLVIVDSHYHPRGQLFTCFYHKSYSGENTASAIARSAAATALSLLVPVFIISCKEKVEEILNMLTARLPGKPSADESQAVQIHMGLALGMFLSRLCEEKLSDMSGQQMSLLLMKSLDALESCCFDPSLEHNTGCILGVGLALSLMSHSSHTESRVHVAASLRKLSAYLDESGSQSRTFQEVLAYTLSCVCTSAFSAGIIEAVEAEETMTKLQLLVENNQQTSGFALALGNLVYGLSVCGHGKAEDLGNRLRPSWIKIVLSEGAPTMLCLAALHGLVALVGSDVDVMQFQSEAIHNSQFQARLNEVIRTLTEVISVSGVIGLQSNAIWLLGHLHLSTLSSNQSRTSVPTDYSYLPEGSFIRAAIGFFITGGKKGPEAVPPSLLKVVMKPIATVGENYQYPPANLAALLSPLMRLNFGEEIQQLCLEIAVSQAPSSQSAAALLGLWVMPPLVHGLSLNIKKYLLVSMPLWAKHVSDEQIQGFVENLMVAVFKPASQPCHPEMCPSALQGLSQAMKLPSPSHHLWSLLCDATGRIFDLLPNRIRRNDLELYISIAKCLSEMTDEGANQVSQITKENIEKAAFVKLYLISQGRLPLMSLTDLLTAAMQHPSKETLAWMILHSLYQARIVNHTNTGVLKRLEWLLELMGYMRNIAYQSASAQNVPPAEALDFLMLIFAAAVVAWADHEAPLLLGLSASWLPWHQENGPGGPAAALLGRSPMHRVTVQEVLTLLPTSMLLLLQKEPWKEQTQKFIDWLFSIMEIPNEAFAATSKDLLKATLLSLRVLPEFKKKAVWTRAYGW

Target ORF information:

RefSeq Version	XM_233144.8
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus focadhesin (Focad), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_233144.8

ORF Insert Sequence:

ATGTCAGATG ATATCAAGAA AAGGTTTGAT TTTCCGAATT CCCTTATCCA GTCCCAGGCT 
GTAGGTCATC TTATTGCTGC TGTCTTAAAA GAACATGGCG CTTCAGAAAA GATACACCAG 
TCCACAAACC AGACTGCTGC CTTGAACTTG CTGTGGGAGA AGAGCTGCAG TGACAATGTG 
GTGGTCCGAA CAGCCTGCAG CGAGGGACTG GTGGCACTTG TTGCTCAGGG TCACGCAGAG 
TTCAGCTATG TTCTCAATGG GATACTCAAC CTGATTCCTT CTGCCAGGAA TATGTCCGGT 
TTGATAAAAG CCCTTATGAA ATTATTACAA ATGCAAGCAT CCAGGGAAGG ACAAGGTGGG 
GCAAAGGATC TTCAGGACCT ATATTCTATC AGAAATCCTC CTCAGCCTTT GATCACCGTG 
CTTGAGCACA GGCCTGATTG CTGGCCACTA CTCCTGCAGC AGCTGACAGC TTTTTTCCAG 
CAATGCCCTG AAAGGTCAGA AGCTTCATGT GTCCAAATAA TGGCACCATT TCTGTGGTAT 
CTCTACTGTG AACCATCCCA GTTACAAGAA TATGCTAAGC TTCGACTGTC CCTGCTCAAA 
GTCTTGCTTC AGCCCAGAGG TGTTTGTGAG GAAGCACAGC CATCAGTGTT GGAACAGCAG 
ATCCTTCAGC TGTGCTGTAA CATGGTTCCG TGCTTGCAGA TGAAAGACCT GATGCAGACA 
ACAGAGATGA CGCTGTTCGT GGAGGAAGTA TATCTAAGCC TTCTGCGCCA TCCTGCTTTC 
TGGAAGAGAC AGCTTACCCA GCTGACCGTT CAGTTGCTGT GCATCTGTGA AGTCAGCTTA 
AAGACAACTG GGGAATGCTC ATCTTTAATT CAGCTTTTAG AGCGCAGTGT TGAACTTCTT 
GGGGAGGATT TTCCTGTCAA ATTGGTCATT ATTGGAATAG CTTTAATACT TCTACAGACT 
CCAGCAAGTC AGCAGAAGCC AGTATTGAAC CTAACTCTGA AGCTCCTCTC GCTTACTGAG 
GGTCAGAGAA TCCCGAAGGC CTCTCTGCTG CTCCTGGTGC CTCTTCTGCA GATTCTGTCC 
TCCACTGCCT TGGAAGACTG TCTGGCCACG GGTGAGGAAG GCCCCTCCAG GCAGCAGTTA 
GCCCTCAGTC TGTTGGAAGT GCTACAGAGA GAGTGTCATG GCGACGACCG ACACACGATC 
TCCTGCAGGC TTGCTTTTCC TGTGAGCAGC ATGTATGGCT CAGTCTTCAC TGCCTGCAGG 
ATTCTTGAAG TCATGGGAGA AGCGTCCGCA GCGAGTGACT GGCTGGCTGC AGTAGAGTCC 
TTGCTCCCTG CCACCACAGT GATCCCCCCA CATGTTTTCT TACTGCTGGC TCACCTGCTT 
GTTGAAGATG GAGGACAGAA TCTTCAGCAG ATACTGAAGG TCACCACACA GTTAGCTCAA 
GCAGATTCCT CTCAGGTACC ACATCTGATT CCAGTTCTGA TGTTCAAATT GGGGAGACCC 
TTAGACCCTG TATCATATAA TCATATATTA TATACTTTGC CCACACTTGG TGTTCACAAG 
GTATGCGTAG GGCAGATTCT GCGGGTGATT CAACTGCTGG GGACCACACC ACGACTAAGA 
GCTGTCACGT TACGCCTGTT GACATCTCTG TGGGAAAAGC AGGATCGTGT GTACCCTGAA 
CTGCAGCGCT TCATGGCCAT GTCTGATGCA CCCTCTCTCT CTGTGGGCAA GGAGCTGCAG 
TGGGAGAAAC TGATTGCAAA AGCTGCATCC ATCAGAGACA TATGTAAACA GAGGCCCTAT 
CAGCATGGTG CAGACATGCT GGCAGCCATT TCTCAGGTGT TGAACGAATG CACCAAGCCT 
GATCAAGCTT CTCCAGCTGC TTTGGTGTTA CAAGGTCTTC ATGCACTCTG CCAGGCTGAG 
GTTGTCTGTA TTCGCTCCAC ATGGAATGCT CTCTCTCCCA AGTTGAGCTG TGACATGAGA 
CCACTTATAT TGAAGACCCT GAGTGAACTA TTCTCTCTGG TTCCTTCCTT AACAGTCAAT 
ACAGTTGAAT ATGAGAATTT TAAAGTTCAA GTCCTCACCT TCCTCTGGAC TCACACTCAA 
AACAAGGATC CCATTGTAGC AAGTGCCGCA TATAAATCTC TGTCTCACTT CAGTGCTGGA 
GAGCACACCA TTCTTCACCT GCCAGAAAAG ATAAGGCCAG AAATGCCGGT TCCCGATGAG 
TTGGATGAGG AGGAATCTGT GGACCTTTCC ATCCCTGGCT CTTGCTATCT CCGACTGCTG 
ACCATCACTG CTCCTTCAAT TTTACCAGCT TTGGAAGAAT TCTTCACGTC TCTTGTGAGA 
CAAGAGATGG TGAATATGCC CCGTGGGATA TACCACTCAG CATTGAAGGG AGGCGTCTGC 
TCAGACCAAG GGAAGACAGT AGCAGGAATC CCCAACTTTA TATTGAAGAC ATATGAAACA 
AACAAGCAAC CAGGACTGAA ACCCGGTCTG GCAGGTGGCA TGCTGTTTTG CTATGATGTG 
GCCATGTATC AGAGCAAAGA TGGTAAGCCA CTGAATCGGC TGATGGCCAG TAGAGGGCGA 
ACCTTCAAGC AGACAACACT GGCCCTTGTT CATGAAGTCC ACGTCCAGCT CTCAGAATGG 
CACCGTGCAA TCTTTCTTCC ACAGGCTTGG CTTGCATACA TGACTCGAGC TTACCATGCC 
ATTTTACAGG GACGGATAGC AGAGCTGGAG CTGCAGTTGC AACATGGCAA GGAGGGCCCC 
GAGGAGGTGC AGTACAAACG AAGCACGGCC TGGCTCTGGG TCAGAGACAT GCTGACAGAT 
GAGATCACCA AGACAGCTGC GAAAGAGAGT CCAGTGGTCA AAGGCAATGC TCTGTTGGCC 
TTAAGCAGCC TTGCCGTCGT GGTGTCCAAG CATGAAGCCA GCCTTTCCTC AGAGTCTGAT 
GGAGTCCTAG AGGTCCAACC TAATTTCCTT CCAGTGAAAG AGTGGGTTTC CATGGTGCTT 
AATACTGTCC TGGTCATTGT GGATAGCCAT TACCACCCCA GAGGTCAACT GTTCACCTGC 
TTTTATCATA AGTCCTATTC TGGCGAGAAC ACGGCAAGTG CTATTGCGCG CTCTGCCGCC 
GCCACGGCTT TGTCTCTCCT CGTGCCGGTT TTCATTATCT CTTGCAAAGA GAAGGTTGAG 
GAAATCCTGA ACATGCTGAC TGCCAGGTTA CCTGGGAAAC CAAGTGCTGA TGAATCTCAA 
GCCGTGCAAA TCCACATGGG CCTTGCCTTG GGGATGTTTC TCTCTCGCCT GTGTGAGGAG 
AAACTCAGTG ACATGTCTGG CCAACAGATG AGCCTTCTTC TGATGAAGTC CTTGGATGCC 
CTGGAGAGCT GCTGTTTCGA CCCCAGTCTT GAACACAACA CCGGCTGTAT ACTGGGAGTT 
GGACTTGCCC TGTCCCTCAT GAGCCACAGC AGCCACACAG AGTCACGCGT TCATGTGGCA 
GCATCCCTGC GGAAGCTGTC TGCCTACCTG GATGAGAGCG GGAGCCAGAG CAGGACATTT 
CAGGAGGTCC TTGCCTACAC ACTTAGCTGT GTGTGTACAT CAGCGTTCAG TGCTGGCATT 
ATTGAGGCTG TGGAGGCTGA AGAGACTATG ACCAAGCTCC AGCTGTTGGT GGAGAACAAC 
CAGCAGACTT CAGGCTTTGC ACTGGCCTTA GGAAACTTGG TTTATGGGTT GTCTGTGTGT 
GGACATGGAA AAGCTGAAGA CTTGGGCAAC AGACTTCGCC CCAGCTGGAT TAAAATTGTC 
CTGTCAGAGG GTGCCCCCAC CATGCTCTGT CTGGCAGCCC TTCATGGCCT GGTGGCACTG 
GTAGGCTCCG ATGTGGATGT CATGCAGTTC CAGTCAGAAG CCATCCACAA CTCCCAGTTT 
CAAGCAAGAC TTAATGAAGT CATTCGAACC TTAACTGAGG TCATCAGTGT CTCAGGGGTG 
ATTGGTCTTC AATCAAATGC AATATGGCTG CTAGGACATC TTCATCTATC CACCTTGTCT 
TCAAATCAAA GCAGAACTTC CGTTCCTACC GACTACAGCT ATTTGCCTGA AGGCAGTTTT 
ATTAGAGCAG CCATTGGCTT CTTCATCACA GGAGGAAAAA AAGGCCCTGA AGCTGTGCCA 
CCGTCCCTTC TCAAAGTAGT GATGAAACCC ATAGCAACTG TTGGGGAAAA CTACCAGTAC 
CCTCCTGCAA ACTTAGCCGC ACTCCTCTCT CCACTTATGA GGCTAAACTT TGGCGAGGAG 
ATACAGCAAC TGTGCCTTGA GATTGCTGTG TCCCAGGCAC CGTCGTCACA GAGTGCAGCT 
GCACTGCTGG GCTTGTGGGT GATGCCGCCG CTGGTCCACG GTCTGAGTCT GAATATCAAG 
AAATACCTCC TGGTATCTAT GCCTCTGTGG GCAAAGCATG TTTCTGATGA ACAGATCCAG 
GGTTTTGTTG AAAATCTGAT GGTGGCAGTT TTTAAACCGG CTTCCCAACC TTGTCATCCT 
GAGATGTGCC CAAGTGCCTT ACAGGGTCTG AGCCAGGCCA TGAAACTGCC CAGTCCTTCC 
CACCACCTCT GGAGTCTGCT CTGCGATGCT ACTGGGAGAA TTTTTGACCT TCTGCCAAAT 
AGGATTCGGA GAAATGATCT CGAGCTGTAC ATCAGTATAG CAAAGTGCCT CTCAGAGATG 
ACGGATGAAG GTGCCAATCA GGTTTCCCAG ATTACTAAGG AAAACATAGA AAAGGCTGCC 
TTTGTCAAGC TGTACTTAAT CTCTCAAGGA CGACTCCCGT TGATGAGCCT GACTGATTTA 
CTCACTGCTG CTATGCAACA CCCCTCGAAG GAGACTCTAG CCTGGATGAT CCTGCACAGC 
TTATACCAGG CACGCATTGT CAATCACACC AACACAGGTG TACTGAAGAG ACTGGAGTGG 
CTCCTGGAGC TGATGGGTTA TATGAGAAAC ATTGCTTACC AGTCGGCGTC TGCTCAGAAT 
GTGCCTCCTG CGGAGGCTTT GGACTTCTTG ATGCTGATAT TTGCTGCTGC AGTGGTAGCA 
TGGGCAGACC ATGAGGCCCC CCTTCTCCTT GGCCTCAGTG CCAGTTGGTT GCCATGGCAC 
CAGGAAAATG GCCCTGGTGG GCCAGCAGCA GCCTTACTTG GCAGGAGTCC GATGCACAGA 
GTCACTGTGC AGGAGGTTCT CACTCTCCTC CCCACGAGCA TGCTACTGCT CCTGCAAAAA 
GAGCCATGGA AGGAACAGAC CCAGAAGTTC ATTGACTGGC TGTTCAGCAT CATGGAAATT 
CCTAACGAAG CCTTTGCAGC CACGTCCAAG GATCTTTTGA AAGCCACCCT GCTGTCCTTG 
AGAGTTCTCC CAGAGTTTAA GAAGAAAGCT GTATGGACCC GAGCGTATGG CTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Focad ( XM_017603023.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017458512.1 Rattus norvegicus focadhesin (Focad), transcript variant X4, mRNA.

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CloneID	ORa16728
Clone ID Related Accession (Same CDS sequence)	XM_017603022.1 , XM_001053590.5 , XM_233144.8 , XM_017603023.1
Accession Version	XM_017603023.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5397bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	focadhesin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000073.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGATG ATATCAAGAA AAGGTTTGAT TTTCCGAATT CCCTTATCCA GTCCCAGGCT 
GTAGGTCATC TTATTGCTGC TGTCTTAAAA GAACATGGCG CTTCAGAAAA GATACACCAG 
TCCACAAACC AGACTGCTGC CTTGAACTTG CTGTGGGAGA AGAGCTGCAG TGACAATGTG 
GTGGTCCGAA CAGCCTGCAG CGAGGGACTG GTGGCACTTG TTGCTCAGGG TCACGCAGAG 
TTCAGCTATG TTCTCAATGG GATACTCAAC CTGATTCCTT CTGCCAGGAA TATGTCCGGT 
TTGATAAAAG CCCTTATGAA ATTATTACAA ATGCAAGCAT CCAGGGAAGG ACAAGGTGGG 
GCAAAGGATC TTCAGGACCT ATATTCTATC AGAAATCCTC CTCAGCCTTT GATCACCGTG 
CTTGAGCACA GGCCTGATTG CTGGCCACTA CTCCTGCAGC AGCTGACAGC TTTTTTCCAG 
CAATGCCCTG AAAGGTCAGA AGCTTCATGT GTCCAAATAA TGGCACCATT TCTGTGGTAT 
CTCTACTGTG AACCATCCCA GTTACAAGAA TATGCTAAGC TTCGACTGTC CCTGCTCAAA 
GTCTTGCTTC AGCCCAGAGG TGTTTGTGAG GAAGCACAGC CATCAGTGTT GGAACAGCAG 
ATCCTTCAGC TGTGCTGTAA CATGGTTCCG TGCTTGCAGA TGAAAGACCT GATGCAGACA 
ACAGAGATGA CGCTGTTCGT GGAGGAAGTA TATCTAAGCC TTCTGCGCCA TCCTGCTTTC 
TGGAAGAGAC AGCTTACCCA GCTGACCGTT CAGTTGCTGT GCATCTGTGA AGTCAGCTTA 
AAGACAACTG GGGAATGCTC ATCTTTAATT CAGCTTTTAG AGCGCAGTGT TGAACTTCTT 
GGGGAGGATT TTCCTGTCAA ATTGGTCATT ATTGGAATAG CTTTAATACT TCTACAGACT 
CCAGCAAGTC AGCAGAAGCC AGTATTGAAC CTAACTCTGA AGCTCCTCTC GCTTACTGAG 
GGTCAGAGAA TCCCGAAGGC CTCTCTGCTG CTCCTGGTGC CTCTTCTGCA GATTCTGTCC 
TCCACTGCCT TGGAAGACTG TCTGGCCACG GGTGAGGAAG GCCCCTCCAG GCAGCAGTTA 
GCCCTCAGTC TGTTGGAAGT GCTACAGAGA GAGTGTCATG GCGACGACCG ACACACGATC 
TCCTGCAGGC TTGCTTTTCC TGTGAGCAGC ATGTATGGCT CAGTCTTCAC TGCCTGCAGG 
ATTCTTGAAG TCATGGGAGA AGCGTCCGCA GCGAGTGACT GGCTGGCTGC AGTAGAGTCC 
TTGCTCCCTG CCACCACAGT GATCCCCCCA CATGTTTTCT TACTGCTGGC TCACCTGCTT 
GTTGAAGATG GAGGACAGAA TCTTCAGCAG ATACTGAAGG TCACCACACA GTTAGCTCAA 
GCAGATTCCT CTCAGGTACC ACATCTGATT CCAGTTCTGA TGTTCAAATT GGGGAGACCC 
TTAGACCCTG TATCATATAA TCATATATTA TATACTTTGC CCACACTTGG TGTTCACAAG 
GTATGCGTAG GGCAGATTCT GCGGGTGATT CAACTGCTGG GGACCACACC ACGACTAAGA 
GCTGTCACGT TACGCCTGTT GACATCTCTG TGGGAAAAGC AGGATCGTGT GTACCCTGAA 
CTGCAGCGCT TCATGGCCAT GTCTGATGCA CCCTCTCTCT CTGTGGGCAA GGAGCTGCAG 
TGGGAGAAAC TGATTGCAAA AGCTGCATCC ATCAGAGACA TATGTAAACA GAGGCCCTAT 
CAGCATGGTG CAGACATGCT GGCAGCCATT TCTCAGGTGT TGAACGAATG CACCAAGCCT 
GATCAAGCTT CTCCAGCTGC TTTGGTGTTA CAAGGTCTTC ATGCACTCTG CCAGGCTGAG 
GTTGTCTGTA TTCGCTCCAC ATGGAATGCT CTCTCTCCCA AGTTGAGCTG TGACATGAGA 
CCACTTATAT TGAAGACCCT GAGTGAACTA TTCTCTCTGG TTCCTTCCTT AACAGTCAAT 
ACAGTTGAAT ATGAGAATTT TAAAGTTCAA GTCCTCACCT TCCTCTGGAC TCACACTCAA 
AACAAGGATC CCATTGTAGC AAGTGCCGCA TATAAATCTC TGTCTCACTT CAGTGCTGGA 
GAGCACACCA TTCTTCACCT GCCAGAAAAG ATAAGGCCAG AAATGCCGGT TCCCGATGAG 
TTGGATGAGG AGGAATCTGT GGACCTTTCC ATCCCTGGCT CTTGCTATCT CCGACTGCTG 
ACCATCACTG CTCCTTCAAT TTTACCAGCT TTGGAAGAAT TCTTCACGTC TCTTGTGAGA 
CAAGAGATGG TGAATATGCC CCGTGGGATA TACCACTCAG CATTGAAGGG AGGCGTCTGC 
TCAGACCAAG GGAAGACAGT AGCAGGAATC CCCAACTTTA TATTGAAGAC ATATGAAACA 
AACAAGCAAC CAGGACTGAA ACCCGGTCTG GCAGGTGGCA TGCTGTTTTG CTATGATGTG 
GCCATGTATC AGAGCAAAGA TGGTAAGCCA CTGAATCGGC TGATGGCCAG TAGAGGGCGA 
ACCTTCAAGC AGACAACACT GGCCCTTGTT CATGAAGTCC ACGTCCAGCT CTCAGAATGG 
CACCGTGCAA TCTTTCTTCC ACAGGCTTGG CTTGCATACA TGACTCGAGC TTACCATGCC 
ATTTTACAGG GACGGATAGC AGAGCTGGAG CTGCAGTTGC AACATGGCAA GGAGGGCCCC 
GAGGAGGTGC AGTACAAACG AAGCACGGCC TGGCTCTGGG TCAGAGACAT GCTGACAGAT 
GAGATCACCA AGACAGCTGC GAAAGAGAGT CCAGTGGTCA AAGGCAATGC TCTGTTGGCC 
TTAAGCAGCC TTGCCGTCGT GGTGTCCAAG CATGAAGCCA GCCTTTCCTC AGAGTCTGAT 
GGAGTCCTAG AGGTCCAACC TAATTTCCTT CCAGTGAAAG AGTGGGTTTC CATGGTGCTT 
AATACTGTCC TGGTCATTGT GGATAGCCAT TACCACCCCA GAGGTCAACT GTTCACCTGC 
TTTTATCATA AGTCCTATTC TGGCGAGAAC ACGGCAAGTG CTATTGCGCG CTCTGCCGCC 
GCCACGGCTT TGTCTCTCCT CGTGCCGGTT TTCATTATCT CTTGCAAAGA GAAGGTTGAG 
GAAATCCTGA ACATGCTGAC TGCCAGGTTA CCTGGGAAAC CAAGTGCTGA TGAATCTCAA 
GCCGTGCAAA TCCACATGGG CCTTGCCTTG GGGATGTTTC TCTCTCGCCT GTGTGAGGAG 
AAACTCAGTG ACATGTCTGG CCAACAGATG AGCCTTCTTC TGATGAAGTC CTTGGATGCC 
CTGGAGAGCT GCTGTTTCGA CCCCAGTCTT GAACACAACA CCGGCTGTAT ACTGGGAGTT 
GGACTTGCCC TGTCCCTCAT GAGCCACAGC AGCCACACAG AGTCACGCGT TCATGTGGCA 
GCATCCCTGC GGAAGCTGTC TGCCTACCTG GATGAGAGCG GGAGCCAGAG CAGGACATTT 
CAGGAGGTCC TTGCCTACAC ACTTAGCTGT GTGTGTACAT CAGCGTTCAG TGCTGGCATT 
ATTGAGGCTG TGGAGGCTGA AGAGACTATG ACCAAGCTCC AGCTGTTGGT GGAGAACAAC 
CAGCAGACTT CAGGCTTTGC ACTGGCCTTA GGAAACTTGG TTTATGGGTT GTCTGTGTGT 
GGACATGGAA AAGCTGAAGA CTTGGGCAAC AGACTTCGCC CCAGCTGGAT TAAAATTGTC 
CTGTCAGAGG GTGCCCCCAC CATGCTCTGT CTGGCAGCCC TTCATGGCCT GGTGGCACTG 
GTAGGCTCCG ATGTGGATGT CATGCAGTTC CAGTCAGAAG CCATCCACAA CTCCCAGTTT 
CAAGCAAGAC TTAATGAAGT CATTCGAACC TTAACTGAGG TCATCAGTGT CTCAGGGGTG 
ATTGGTCTTC AATCAAATGC AATATGGCTG CTAGGACATC TTCATCTATC CACCTTGTCT 
TCAAATCAAA GCAGAACTTC CGTTCCTACC GACTACAGCT ATTTGCCTGA AGGCAGTTTT 
ATTAGAGCAG CCATTGGCTT CTTCATCACA GGAGGAAAAA AAGGCCCTGA AGCTGTGCCA 
CCGTCCCTTC TCAAAGTAGT GATGAAACCC ATAGCAACTG TTGGGGAAAA CTACCAGTAC 
CCTCCTGCAA ACTTAGCCGC ACTCCTCTCT CCACTTATGA GGCTAAACTT TGGCGAGGAG 
ATACAGCAAC TGTGCCTTGA GATTGCTGTG TCCCAGGCAC CGTCGTCACA GAGTGCAGCT 
GCACTGCTGG GCTTGTGGGT GATGCCGCCG CTGGTCCACG GTCTGAGTCT GAATATCAAG 
AAATACCTCC TGGTATCTAT GCCTCTGTGG GCAAAGCATG TTTCTGATGA ACAGATCCAG 
GGTTTTGTTG AAAATCTGAT GGTGGCAGTT TTTAAACCGG CTTCCCAACC TTGTCATCCT 
GAGATGTGCC CAAGTGCCTT ACAGGGTCTG AGCCAGGCCA TGAAACTGCC CAGTCCTTCC 
CACCACCTCT GGAGTCTGCT CTGCGATGCT ACTGGGAGAA TTTTTGACCT TCTGCCAAAT 
AGGATTCGGA GAAATGATCT CGAGCTGTAC ATCAGTATAG CAAAGTGCCT CTCAGAGATG 
ACGGATGAAG GTGCCAATCA GGTTTCCCAG ATTACTAAGG AAAACATAGA AAAGGCTGCC 
TTTGTCAAGC TGTACTTAAT CTCTCAAGGA CGACTCCCGT TGATGAGCCT GACTGATTTA 
CTCACTGCTG CTATGCAACA CCCCTCGAAG GAGACTCTAG CCTGGATGAT CCTGCACAGC 
TTATACCAGG CACGCATTGT CAATCACACC AACACAGGTG TACTGAAGAG ACTGGAGTGG 
CTCCTGGAGC TGATGGGTTA TATGAGAAAC ATTGCTTACC AGTCGGCGTC TGCTCAGAAT 
GTGCCTCCTG CGGAGGCTTT GGACTTCTTG ATGCTGATAT TTGCTGCTGC AGTGGTAGCA 
TGGGCAGACC ATGAGGCCCC CCTTCTCCTT GGCCTCAGTG CCAGTTGGTT GCCATGGCAC 
CAGGAAAATG GCCCTGGTGG GCCAGCAGCA GCCTTACTTG GCAGGAGTCC GATGCACAGA 
GTCACTGTGC AGGAGGTTCT CACTCTCCTC CCCACGAGCA TGCTACTGCT CCTGCAAAAA 
GAGCCATGGA AGGAACAGAC CCAGAAGTTC ATTGACTGGC TGTTCAGCAT CATGGAAATT 
CCTAACGAAG CCTTTGCAGC CACGTCCAAG GATCTTTTGA AAGCCACCCT GCTGTCCTTG 
AGAGTTCTCC CAGAGTTTAA GAAGAAAGCT GTATGGACCC GAGCGTATGG CTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017458512.1
CDS	1330..6726
Translation	MSDDIKKRFDFPNSLIQSQAVGHLIAAVLKEHGASEKIHQSTNQTAALNLLWEKSCSDNVVVRTACSEGLVALVAQGHAEFSYVLNGILNLIPSARNMSGLIKALMKLLQMQASREGQGGAKDLQDLYSIRNPPQPLITVLEHRPDCWPLLLQQLTAFFQQCPERSEASCVQIMAPFLWYLYCEPSQLQEYAKLRLSLLKVLLQPRGVCEEAQPSVLEQQILQLCCNMVPCLQMKDLMQTTEMTLFVEEVYLSLLRHPAFWKRQLTQLTVQLLCICEVSLKTTGECSSLIQLLERSVELLGEDFPVKLVIIGIALILLQTPASQQKPVLNLTLKLLSLTEGQRIPKASLLLLVPLLQILSSTALEDCLATGEEGPSRQQLALSLLEVLQRECHGDDRHTISCRLAFPVSSMYGSVFTACRILEVMGEASAASDWLAAVESLLPATTVIPPHVFLLLAHLLVEDGGQNLQQILKVTTQLAQADSSQVPHLIPVLMFKLGRPLDPVSYNHILYTLPTLGVHKVCVGQILRVIQLLGTTPRLRAVTLRLLTSLWEKQDRVYPELQRFMAMSDAPSLSVGKELQWEKLIAKAASIRDICKQRPYQHGADMLAAISQVLNECTKPDQASPAALVLQGLHALCQAEVVCIRSTWNALSPKLSCDMRPLILKTLSELFSLVPSLTVNTVEYENFKVQVLTFLWTHTQNKDPIVASAAYKSLSHFSAGEHTILHLPEKIRPEMPVPDELDEEESVDLSIPGSCYLRLLTITAPSILPALEEFFTSLVRQEMVNMPRGIYHSALKGGVCSDQGKTVAGIPNFILKTYETNKQPGLKPGLAGGMLFCYDVAMYQSKDGKPLNRLMASRGRTFKQTTLALVHEVHVQLSEWHRAIFLPQAWLAYMTRAYHAILQGRIAELELQLQHGKEGPEEVQYKRSTAWLWVRDMLTDEITKTAAKESPVVKGNALLALSSLAVVVSKHEASLSSESDGVLEVQPNFLPVKEWVSMVLNTVLVIVDSHYHPRGQLFTCFYHKSYSGENTASAIARSAAATALSLLVPVFIISCKEKVEEILNMLTARLPGKPSADESQAVQIHMGLALGMFLSRLCEEKLSDMSGQQMSLLLMKSLDALESCCFDPSLEHNTGCILGVGLALSLMSHSSHTESRVHVAASLRKLSAYLDESGSQSRTFQEVLAYTLSCVCTSAFSAGIIEAVEAEETMTKLQLLVENNQQTSGFALALGNLVYGLSVCGHGKAEDLGNRLRPSWIKIVLSEGAPTMLCLAALHGLVALVGSDVDVMQFQSEAIHNSQFQARLNEVIRTLTEVISVSGVIGLQSNAIWLLGHLHLSTLSSNQSRTSVPTDYSYLPEGSFIRAAIGFFITGGKKGPEAVPPSLLKVVMKPIATVGENYQYPPANLAALLSPLMRLNFGEEIQQLCLEIAVSQAPSSQSAAALLGLWVMPPLVHGLSLNIKKYLLVSMPLWAKHVSDEQIQGFVENLMVAVFKPASQPCHPEMCPSALQGLSQAMKLPSPSHHLWSLLCDATGRIFDLLPNRIRRNDLELYISIAKCLSEMTDEGANQVSQITKENIEKAAFVKLYLISQGRLPLMSLTDLLTAAMQHPSKETLAWMILHSLYQARIVNHTNTGVLKRLEWLLELMGYMRNIAYQSASAQNVPPAEALDFLMLIFAAAVVAWADHEAPLLLGLSASWLPWHQENGPGGPAAALLGRSPMHRVTVQEVLTLLPTSMLLLLQKEPWKEQTQKFIDWLFSIMEIPNEAFAATSKDLLKATLLSLRVLPEFKKKAVWTRAYGW

Target ORF information:

RefSeq Version	XM_017603023.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus focadhesin (Focad), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017603023.1

ORF Insert Sequence:

ATGTCAGATG ATATCAAGAA AAGGTTTGAT TTTCCGAATT CCCTTATCCA GTCCCAGGCT 
GTAGGTCATC TTATTGCTGC TGTCTTAAAA GAACATGGCG CTTCAGAAAA GATACACCAG 
TCCACAAACC AGACTGCTGC CTTGAACTTG CTGTGGGAGA AGAGCTGCAG TGACAATGTG 
GTGGTCCGAA CAGCCTGCAG CGAGGGACTG GTGGCACTTG TTGCTCAGGG TCACGCAGAG 
TTCAGCTATG TTCTCAATGG GATACTCAAC CTGATTCCTT CTGCCAGGAA TATGTCCGGT 
TTGATAAAAG CCCTTATGAA ATTATTACAA ATGCAAGCAT CCAGGGAAGG ACAAGGTGGG 
GCAAAGGATC TTCAGGACCT ATATTCTATC AGAAATCCTC CTCAGCCTTT GATCACCGTG 
CTTGAGCACA GGCCTGATTG CTGGCCACTA CTCCTGCAGC AGCTGACAGC TTTTTTCCAG 
CAATGCCCTG AAAGGTCAGA AGCTTCATGT GTCCAAATAA TGGCACCATT TCTGTGGTAT 
CTCTACTGTG AACCATCCCA GTTACAAGAA TATGCTAAGC TTCGACTGTC CCTGCTCAAA 
GTCTTGCTTC AGCCCAGAGG TGTTTGTGAG GAAGCACAGC CATCAGTGTT GGAACAGCAG 
ATCCTTCAGC TGTGCTGTAA CATGGTTCCG TGCTTGCAGA TGAAAGACCT GATGCAGACA 
ACAGAGATGA CGCTGTTCGT GGAGGAAGTA TATCTAAGCC TTCTGCGCCA TCCTGCTTTC 
TGGAAGAGAC AGCTTACCCA GCTGACCGTT CAGTTGCTGT GCATCTGTGA AGTCAGCTTA 
AAGACAACTG GGGAATGCTC ATCTTTAATT CAGCTTTTAG AGCGCAGTGT TGAACTTCTT 
GGGGAGGATT TTCCTGTCAA ATTGGTCATT ATTGGAATAG CTTTAATACT TCTACAGACT 
CCAGCAAGTC AGCAGAAGCC AGTATTGAAC CTAACTCTGA AGCTCCTCTC GCTTACTGAG 
GGTCAGAGAA TCCCGAAGGC CTCTCTGCTG CTCCTGGTGC CTCTTCTGCA GATTCTGTCC 
TCCACTGCCT TGGAAGACTG TCTGGCCACG GGTGAGGAAG GCCCCTCCAG GCAGCAGTTA 
GCCCTCAGTC TGTTGGAAGT GCTACAGAGA GAGTGTCATG GCGACGACCG ACACACGATC 
TCCTGCAGGC TTGCTTTTCC TGTGAGCAGC ATGTATGGCT CAGTCTTCAC TGCCTGCAGG 
ATTCTTGAAG TCATGGGAGA AGCGTCCGCA GCGAGTGACT GGCTGGCTGC AGTAGAGTCC 
TTGCTCCCTG CCACCACAGT GATCCCCCCA CATGTTTTCT TACTGCTGGC TCACCTGCTT 
GTTGAAGATG GAGGACAGAA TCTTCAGCAG ATACTGAAGG TCACCACACA GTTAGCTCAA 
GCAGATTCCT CTCAGGTACC ACATCTGATT CCAGTTCTGA TGTTCAAATT GGGGAGACCC 
TTAGACCCTG TATCATATAA TCATATATTA TATACTTTGC CCACACTTGG TGTTCACAAG 
GTATGCGTAG GGCAGATTCT GCGGGTGATT CAACTGCTGG GGACCACACC ACGACTAAGA 
GCTGTCACGT TACGCCTGTT GACATCTCTG TGGGAAAAGC AGGATCGTGT GTACCCTGAA 
CTGCAGCGCT TCATGGCCAT GTCTGATGCA CCCTCTCTCT CTGTGGGCAA GGAGCTGCAG 
TGGGAGAAAC TGATTGCAAA AGCTGCATCC ATCAGAGACA TATGTAAACA GAGGCCCTAT 
CAGCATGGTG CAGACATGCT GGCAGCCATT TCTCAGGTGT TGAACGAATG CACCAAGCCT 
GATCAAGCTT CTCCAGCTGC TTTGGTGTTA CAAGGTCTTC ATGCACTCTG CCAGGCTGAG 
GTTGTCTGTA TTCGCTCCAC ATGGAATGCT CTCTCTCCCA AGTTGAGCTG TGACATGAGA 
CCACTTATAT TGAAGACCCT GAGTGAACTA TTCTCTCTGG TTCCTTCCTT AACAGTCAAT 
ACAGTTGAAT ATGAGAATTT TAAAGTTCAA GTCCTCACCT TCCTCTGGAC TCACACTCAA 
AACAAGGATC CCATTGTAGC AAGTGCCGCA TATAAATCTC TGTCTCACTT CAGTGCTGGA 
GAGCACACCA TTCTTCACCT GCCAGAAAAG ATAAGGCCAG AAATGCCGGT TCCCGATGAG 
TTGGATGAGG AGGAATCTGT GGACCTTTCC ATCCCTGGCT CTTGCTATCT CCGACTGCTG 
ACCATCACTG CTCCTTCAAT TTTACCAGCT TTGGAAGAAT TCTTCACGTC TCTTGTGAGA 
CAAGAGATGG TGAATATGCC CCGTGGGATA TACCACTCAG CATTGAAGGG AGGCGTCTGC 
TCAGACCAAG GGAAGACAGT AGCAGGAATC CCCAACTTTA TATTGAAGAC ATATGAAACA 
AACAAGCAAC CAGGACTGAA ACCCGGTCTG GCAGGTGGCA TGCTGTTTTG CTATGATGTG 
GCCATGTATC AGAGCAAAGA TGGTAAGCCA CTGAATCGGC TGATGGCCAG TAGAGGGCGA 
ACCTTCAAGC AGACAACACT GGCCCTTGTT CATGAAGTCC ACGTCCAGCT CTCAGAATGG 
CACCGTGCAA TCTTTCTTCC ACAGGCTTGG CTTGCATACA TGACTCGAGC TTACCATGCC 
ATTTTACAGG GACGGATAGC AGAGCTGGAG CTGCAGTTGC AACATGGCAA GGAGGGCCCC 
GAGGAGGTGC AGTACAAACG AAGCACGGCC TGGCTCTGGG TCAGAGACAT GCTGACAGAT 
GAGATCACCA AGACAGCTGC GAAAGAGAGT CCAGTGGTCA AAGGCAATGC TCTGTTGGCC 
TTAAGCAGCC TTGCCGTCGT GGTGTCCAAG CATGAAGCCA GCCTTTCCTC AGAGTCTGAT 
GGAGTCCTAG AGGTCCAACC TAATTTCCTT CCAGTGAAAG AGTGGGTTTC CATGGTGCTT 
AATACTGTCC TGGTCATTGT GGATAGCCAT TACCACCCCA GAGGTCAACT GTTCACCTGC 
TTTTATCATA AGTCCTATTC TGGCGAGAAC ACGGCAAGTG CTATTGCGCG CTCTGCCGCC 
GCCACGGCTT TGTCTCTCCT CGTGCCGGTT TTCATTATCT CTTGCAAAGA GAAGGTTGAG 
GAAATCCTGA ACATGCTGAC TGCCAGGTTA CCTGGGAAAC CAAGTGCTGA TGAATCTCAA 
GCCGTGCAAA TCCACATGGG CCTTGCCTTG GGGATGTTTC TCTCTCGCCT GTGTGAGGAG 
AAACTCAGTG ACATGTCTGG CCAACAGATG AGCCTTCTTC TGATGAAGTC CTTGGATGCC 
CTGGAGAGCT GCTGTTTCGA CCCCAGTCTT GAACACAACA CCGGCTGTAT ACTGGGAGTT 
GGACTTGCCC TGTCCCTCAT GAGCCACAGC AGCCACACAG AGTCACGCGT TCATGTGGCA 
GCATCCCTGC GGAAGCTGTC TGCCTACCTG GATGAGAGCG GGAGCCAGAG CAGGACATTT 
CAGGAGGTCC TTGCCTACAC ACTTAGCTGT GTGTGTACAT CAGCGTTCAG TGCTGGCATT 
ATTGAGGCTG TGGAGGCTGA AGAGACTATG ACCAAGCTCC AGCTGTTGGT GGAGAACAAC 
CAGCAGACTT CAGGCTTTGC ACTGGCCTTA GGAAACTTGG TTTATGGGTT GTCTGTGTGT 
GGACATGGAA AAGCTGAAGA CTTGGGCAAC AGACTTCGCC CCAGCTGGAT TAAAATTGTC 
CTGTCAGAGG GTGCCCCCAC CATGCTCTGT CTGGCAGCCC TTCATGGCCT GGTGGCACTG 
GTAGGCTCCG ATGTGGATGT CATGCAGTTC CAGTCAGAAG CCATCCACAA CTCCCAGTTT 
CAAGCAAGAC TTAATGAAGT CATTCGAACC TTAACTGAGG TCATCAGTGT CTCAGGGGTG 
ATTGGTCTTC AATCAAATGC AATATGGCTG CTAGGACATC TTCATCTATC CACCTTGTCT 
TCAAATCAAA GCAGAACTTC CGTTCCTACC GACTACAGCT ATTTGCCTGA AGGCAGTTTT 
ATTAGAGCAG CCATTGGCTT CTTCATCACA GGAGGAAAAA AAGGCCCTGA AGCTGTGCCA 
CCGTCCCTTC TCAAAGTAGT GATGAAACCC ATAGCAACTG TTGGGGAAAA CTACCAGTAC 
CCTCCTGCAA ACTTAGCCGC ACTCCTCTCT CCACTTATGA GGCTAAACTT TGGCGAGGAG 
ATACAGCAAC TGTGCCTTGA GATTGCTGTG TCCCAGGCAC CGTCGTCACA GAGTGCAGCT 
GCACTGCTGG GCTTGTGGGT GATGCCGCCG CTGGTCCACG GTCTGAGTCT GAATATCAAG 
AAATACCTCC TGGTATCTAT GCCTCTGTGG GCAAAGCATG TTTCTGATGA ACAGATCCAG 
GGTTTTGTTG AAAATCTGAT GGTGGCAGTT TTTAAACCGG CTTCCCAACC TTGTCATCCT 
GAGATGTGCC CAAGTGCCTT ACAGGGTCTG AGCCAGGCCA TGAAACTGCC CAGTCCTTCC 
CACCACCTCT GGAGTCTGCT CTGCGATGCT ACTGGGAGAA TTTTTGACCT TCTGCCAAAT 
AGGATTCGGA GAAATGATCT CGAGCTGTAC ATCAGTATAG CAAAGTGCCT CTCAGAGATG 
ACGGATGAAG GTGCCAATCA GGTTTCCCAG ATTACTAAGG AAAACATAGA AAAGGCTGCC 
TTTGTCAAGC TGTACTTAAT CTCTCAAGGA CGACTCCCGT TGATGAGCCT GACTGATTTA 
CTCACTGCTG CTATGCAACA CCCCTCGAAG GAGACTCTAG CCTGGATGAT CCTGCACAGC 
TTATACCAGG CACGCATTGT CAATCACACC AACACAGGTG TACTGAAGAG ACTGGAGTGG 
CTCCTGGAGC TGATGGGTTA TATGAGAAAC ATTGCTTACC AGTCGGCGTC TGCTCAGAAT 
GTGCCTCCTG CGGAGGCTTT GGACTTCTTG ATGCTGATAT TTGCTGCTGC AGTGGTAGCA 
TGGGCAGACC ATGAGGCCCC CCTTCTCCTT GGCCTCAGTG CCAGTTGGTT GCCATGGCAC 
CAGGAAAATG GCCCTGGTGG GCCAGCAGCA GCCTTACTTG GCAGGAGTCC GATGCACAGA 
GTCACTGTGC AGGAGGTTCT CACTCTCCTC CCCACGAGCA TGCTACTGCT CCTGCAAAAA 
GAGCCATGGA AGGAACAGAC CCAGAAGTTC ATTGACTGGC TGTTCAGCAT CATGGAAATT 
CCTAACGAAG CCTTTGCAGC CACGTCCAAG GATCTTTTGA AAGCCACCCT GCTGTCCTTG 
AGAGTTCTCC CAGAGTTTAA GAAGAAAGCT GTATGGACCC GAGCGTATGG CTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Focad ( XM_017603024.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017458513.1 Rattus norvegicus focadhesin (Focad), transcript variant X1, mRNA.

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CloneID	ORa55473
Clone ID Related Accession (Same CDS sequence)	XM_017603024.1 , XM_017593754.1
Accession Version	XM_017603024.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5106bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	focadhesin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000073.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGTT TGATAAAAGC CCTTATGAAA TTATTACAAA TGCAAGCATC CAGGGAAGGA 
CAAGGTGGGG CAAAGGATCT TCAGGACCTA TATTCTATCA GAAATCCTCC TCAGCCTTTG 
ATCACCGTGC TTGAGCACAG GCCTGATTGC TGGCCACTAC TCCTGCAGCA GCTGACAGCT 
TTTTTCCAGC AATGCCCTGA AAGGTCAGAA GCTTCATGTG TCCAAATAAT GGCACCATTT 
CTGTGGTATC TCTACTGTGA ACCATCCCAG TTACAAGAAT ATGCTAAGCT TCGACTGTCC 
CTGCTCAAAG TCTTGCTTCA GCCCAGAGGT GTTTGTGAGG AAGCACAGCC ATCAGTGTTG 
GAACAGCAGA TCCTTCAGCT GTGCTGTAAC ATGGTTCCGT GCTTGCAGAT GAAAGACCTG 
ATGCAGACAA CAGAGATGAC GCTGTTCGTG GAGGAAGTAT ATCTAAGCCT TCTGCGCCAT 
CCTGCTTTCT GGAAGAGACA GCTTACCCAG CTGACCGTTC AGTTGCTGTG CATCTGTGAA 
GTCAGCTTAA AGACAACTGG GGAATGCTCA TCTTTAATTC AGCTTTTAGA GCGCAGTGTT 
GAACTTCTTG GGGAGGATTT TCCTGTCAAA TTGGTCATTA TTGGAATAGC TTTAATACTT 
CTACAGACTC CAGCAAGTCA GCAGAAGCCA GTATTGAACC TAACTCTGAA GCTCCTCTCG 
CTTACTGAGG GTCAGAGAAT CCCGAAGGCC TCTCTGCTGC TCCTGGTGCC TCTTCTGCAG 
ATTCTGTCCT CCACTGCCTT GGAAGACTGT CTGGCCACGG GTGAGGAAGG CCCCTCCAGG 
CAGCAGTTAG CCCTCAGTCT GTTGGAAGTG CTACAGAGAG AGTGTCATGG CGACGACCGA 
CACACGATCT CCTGCAGGCT TGCTTTTCCT GTGAGCAGCA TGTATGGCTC AGTCTTCACT 
GCCTGCAGGA TTCTTGAAGT CATGGGAGAA GCGTCCGCAG CGAGTGACTG GCTGGCTGCA 
GTAGAGTCCT TGCTCCCTGC CACCACAGTG ATCCCCCCAC ATGTTTTCTT ACTGCTGGCT 
CACCTGCTTG TTGAAGATGG AGGACAGAAT CTTCAGCAGA TACTGAAGGT CACCACACAG 
TTAGCTCAAG CAGATTCCTC TCAGGTACCA CATCTGATTC CAGTTCTGAT GTTCAAATTG 
GGGAGACCCT TAGACCCTGT ATCATATAAT CATATATTAT ATACTTTGCC CACACTTGGT 
GTTCACAAGG TATGCGTAGG GCAGATTCTG CGGGTGATTC AACTGCTGGG GACCACACCA 
CGACTAAGAG CTGTCACGTT ACGCCTGTTG ACATCTCTGT GGGAAAAGCA GGATCGTGTG 
TACCCTGAAC TGCAGCGCTT CATGGCCATG TCTGATGCAC CCTCTCTCTC TGTGGGCAAG 
GAGCTGCAGT GGGAGAAACT GATTGCAAAA GCTGCATCCA TCAGAGACAT ATGTAAACAG 
AGGCCCTATC AGCATGGTGC AGACATGCTG GCAGCCATTT CTCAGGTGTT GAACGAATGC 
ACCAAGCCTG ATCAAGCTTC TCCAGCTGCT TTGGTGTTAC AAGGTCTTCA TGCACTCTGC 
CAGGCTGAGG TTGTCTGTAT TCGCTCCACA TGGAATGCTC TCTCTCCCAA GTTGAGCTGT 
GACATGAGAC CACTTATATT GAAGACCCTG AGTGAACTAT TCTCTCTGGT TCCTTCCTTA 
ACAGTCAATA CAGTTGAATA TGAGAATTTT AAAGTTCAAG TCCTCACCTT CCTCTGGACT 
CACACTCAAA ACAAGGATCC CATTGTAGCA AGTGCCGCAT ATAAATCTCT GTCTCACTTC 
AGTGCTGGAG AGCACACCAT TCTTCACCTG CCAGAAAAGA TAAGGCCAGA AATGCCGGTT 
CCCGATGAGT TGGATGAGGA GGAATCTGTG GACCTTTCCA TCCCTGGCTC TTGCTATCTC 
CGACTGCTGA CCATCACTGC TCCTTCAATT TTACCAGCTT TGGAAGAATT CTTCACGTCT 
CTTGTGAGAC AAGAGATGGT GAATATGCCC CGTGGGATAT ACCACTCAGC ATTGAAGGGA 
GGCGTCTGCT CAGACCAAGG GAAGACAGTA GCAGGAATCC CCAACTTTAT ATTGAAGACA 
TATGAAACAA ACAAGCAACC AGGACTGAAA CCCGGTCTGG CAGGTGGCAT GCTGTTTTGC 
TATGATGTGG CCATGTATCA GAGCAAAGAT GGTAAGCCAC TGAATCGGCT GATGGCCAGT 
AGAGGGCGAA CCTTCAAGCA GACAACACTG GCCCTTGTTC ATGAAGTCCA CGTCCAGCTC 
TCAGAATGGC ACCGTGCAAT CTTTCTTCCA CAGGCTTGGC TTGCATACAT GACTCGAGCT 
TACCATGCCA TTTTACAGGG ACGGATAGCA GAGCTGGAGC TGCAGTTGCA ACATGGCAAG 
GAGGGCCCCG AGGAGGTGCA GTACAAACGA AGCACGGCCT GGCTCTGGGT CAGAGACATG 
CTGACAGATG AGATCACCAA GACAGCTGCG AAAGAGAGTC CAGTGGTCAA AGGCAATGCT 
CTGTTGGCCT TAAGCAGCCT TGCCGTCGTG GTGTCCAAGC ATGAAGCCAG CCTTTCCTCA 
GAGTCTGATG GAGTCCTAGA GGTCCAACCT AATTTCCTTC CAGTGAAAGA GTGGGTTTCC 
ATGGTGCTTA ATACTGTCCT GGTCATTGTG GATAGCCATT ACCACCCCAG AGGTCAACTG 
TTCACCTGCT TTTATCATAA GTCCTATTCT GGCGAGAACA CGGCAAGTGC TATTGCGCGC 
TCTGCCGCCG CCACGGCTTT GTCTCTCCTC GTGCCGGTTT TCATTATCTC TTGCAAAGAG 
AAGGTTGAGG AAATCCTGAA CATGCTGACT GCCAGGTTAC CTGGGAAACC AAGTGCTGAT 
GAATCTCAAG CCGTGCAAAT CCACATGGGC CTTGCCTTGG GGATGTTTCT CTCTCGCCTG 
TGTGAGGAGA AACTCAGTGA CATGTCTGGC CAACAGATGA GCCTTCTTCT GATGAAGTCC 
TTGGATGCCC TGGAGAGCTG CTGTTTCGAC CCCAGTCTTG AACACAACAC CGGCTGTATA 
CTGGGAGTTG GACTTGCCCT GTCCCTCATG AGCCACAGCA GCCACACAGA GTCACGCGTT 
CATGTGGCAG CATCCCTGCG GAAGCTGTCT GCCTACCTGG ATGAGAGCGG GAGCCAGAGC 
AGGACATTTC AGGAGGTCCT TGCCTACACA CTTAGCTGTG TGTGTACATC AGCGTTCAGT 
GCTGGCATTA TTGAGGCTGT GGAGGCTGAA GAGACTATGA CCAAGCTCCA GCTGTTGGTG 
GAGAACAACC AGCAGACTTC AGGCTTTGCA CTGGCCTTAG GAAACTTGGT TTATGGGTTG 
TCTGTGTGTG GACATGGAAA AGCTGAAGAC TTGGGCAACA GACTTCGCCC CAGCTGGATT 
AAAATTGTCC TGTCAGAGGG TGCCCCCACC ATGCTCTGTC TGGCAGCCCT TCATGGCCTG 
GTGGCACTGG TAGGCTCCGA TGTGGATGTC ATGCAGTTCC AGTCAGAAGC CATCCACAAC 
TCCCAGTTTC AAGCAAGACT TAATGAAGTC ATTCGAACCT TAACTGAGGT CATCAGTGTC 
TCAGGGGTGA TTGGTCTTCA ATCAAATGCA ATATGGCTGC TAGGACATCT TCATCTATCC 
ACCTTGTCTT CAAATCAAAG CAGAACTTCC GTTCCTACCG ACTACAGCTA TTTGCCTGAA 
GGCAGTTTTA TTAGAGCAGC CATTGGCTTC TTCATCACAG GAGGAAAAAA AGGCCCTGAA 
GCTGTGCCAC CGTCCCTTCT CAAAGTAGTG ATGAAACCCA TAGCAACTGT TGGGGAAAAC 
TACCAGTACC CTCCTGCAAA CTTAGCCGCA CTCCTCTCTC CACTTATGAG GCTAAACTTT 
GGCGAGGAGA TACAGCAACT GTGCCTTGAG ATTGCTGTGT CCCAGGCACC GTCGTCACAG 
AGTGCAGCTG CACTGCTGGG CTTGTGGGTG ATGCCGCCGC TGGTCCACGG TCTGAGTCTG 
AATATCAAGA AATACCTCCT GGTATCTATG CCTCTGTGGG CAAAGCATGT TTCTGATGAA 
CAGATCCAGG GTTTTGTTGA AAATCTGATG GTGGCAGTTT TTAAACCGGC TTCCCAACCT 
TGTCATCCTG AGATGTGCCC AAGTGCCTTA CAGGGTCTGA GCCAGGCCAT GAAACTGCCC 
AGTCCTTCCC ACCACCTCTG GAGTCTGCTC TGCGATGCTA CTGGGAGAAT TTTTGACCTT 
CTGCCAAATA GGATTCGGAG AAATGATCTC GAGCTGTACA TCAGTATAGC AAAGTGCCTC 
TCAGAGATGA CGGATGAAGG TGCCAATCAG GTTTCCCAGA TTACTAAGGA AAACATAGAA 
AAGGCTGCCT TTGTCAAGCT GTACTTAATC TCTCAAGGAC GACTCCCGTT GATGAGCCTG 
ACTGATTTAC TCACTGCTGC TATGCAACAC CCCTCGAAGG AGACTCTAGC CTGGATGATC 
CTGCACAGCT TATACCAGGC ACGCATTGTC AATCACACCA ACACAGGTGT ACTGAAGAGA 
CTGGAGTGGC TCCTGGAGCT GATGGGTTAT ATGAGAAACA TTGCTTACCA GTCGGCGTCT 
GCTCAGAATG TGCCTCCTGC GGAGGCTTTG GACTTCTTGA TGCTGATATT TGCTGCTGCA 
GTGGTAGCAT GGGCAGACCA TGAGGCCCCC CTTCTCCTTG GCCTCAGTGC CAGTTGGTTG 
CCATGGCACC AGGAAAATGG CCCTGGTGGG CCAGCAGCAG CCTTACTTGG CAGGAGTCCG 
ATGCACAGAG TCACTGTGCA GGAGGTTCTC ACTCTCCTCC CCACGAGCAT GCTACTGCTC 
CTGCAAAAAG AGCCATGGAA GGAACAGACC CAGAAGTTCA TTGACTGGCT GTTCAGCATC 
ATGGAAATTC CTAACGAAGC CTTTGCAGCC ACGTCCAAGG ATCTTTTGAA AGCCACCCTG 
CTGTCCTTGA GAGTTCTCCC AGAGTTTAAG AAGAAAGCTG TATGGACCCG AGCGTATGGC 
TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017458513.1
CDS	845..5950
Translation	MSGLIKALMKLLQMQASREGQGGAKDLQDLYSIRNPPQPLITVLEHRPDCWPLLLQQLTAFFQQCPERSEASCVQIMAPFLWYLYCEPSQLQEYAKLRLSLLKVLLQPRGVCEEAQPSVLEQQILQLCCNMVPCLQMKDLMQTTEMTLFVEEVYLSLLRHPAFWKRQLTQLTVQLLCICEVSLKTTGECSSLIQLLERSVELLGEDFPVKLVIIGIALILLQTPASQQKPVLNLTLKLLSLTEGQRIPKASLLLLVPLLQILSSTALEDCLATGEEGPSRQQLALSLLEVLQRECHGDDRHTISCRLAFPVSSMYGSVFTACRILEVMGEASAASDWLAAVESLLPATTVIPPHVFLLLAHLLVEDGGQNLQQILKVTTQLAQADSSQVPHLIPVLMFKLGRPLDPVSYNHILYTLPTLGVHKVCVGQILRVIQLLGTTPRLRAVTLRLLTSLWEKQDRVYPELQRFMAMSDAPSLSVGKELQWEKLIAKAASIRDICKQRPYQHGADMLAAISQVLNECTKPDQASPAALVLQGLHALCQAEVVCIRSTWNALSPKLSCDMRPLILKTLSELFSLVPSLTVNTVEYENFKVQVLTFLWTHTQNKDPIVASAAYKSLSHFSAGEHTILHLPEKIRPEMPVPDELDEEESVDLSIPGSCYLRLLTITAPSILPALEEFFTSLVRQEMVNMPRGIYHSALKGGVCSDQGKTVAGIPNFILKTYETNKQPGLKPGLAGGMLFCYDVAMYQSKDGKPLNRLMASRGRTFKQTTLALVHEVHVQLSEWHRAIFLPQAWLAYMTRAYHAILQGRIAELELQLQHGKEGPEEVQYKRSTAWLWVRDMLTDEITKTAAKESPVVKGNALLALSSLAVVVSKHEASLSSESDGVLEVQPNFLPVKEWVSMVLNTVLVIVDSHYHPRGQLFTCFYHKSYSGENTASAIARSAAATALSLLVPVFIISCKEKVEEILNMLTARLPGKPSADESQAVQIHMGLALGMFLSRLCEEKLSDMSGQQMSLLLMKSLDALESCCFDPSLEHNTGCILGVGLALSLMSHSSHTESRVHVAASLRKLSAYLDESGSQSRTFQEVLAYTLSCVCTSAFSAGIIEAVEAEETMTKLQLLVENNQQTSGFALALGNLVYGLSVCGHGKAEDLGNRLRPSWIKIVLSEGAPTMLCLAALHGLVALVGSDVDVMQFQSEAIHNSQFQARLNEVIRTLTEVISVSGVIGLQSNAIWLLGHLHLSTLSSNQSRTSVPTDYSYLPEGSFIRAAIGFFITGGKKGPEAVPPSLLKVVMKPIATVGENYQYPPANLAALLSPLMRLNFGEEIQQLCLEIAVSQAPSSQSAAALLGLWVMPPLVHGLSLNIKKYLLVSMPLWAKHVSDEQIQGFVENLMVAVFKPASQPCHPEMCPSALQGLSQAMKLPSPSHHLWSLLCDATGRIFDLLPNRIRRNDLELYISIAKCLSEMTDEGANQVSQITKENIEKAAFVKLYLISQGRLPLMSLTDLLTAAMQHPSKETLAWMILHSLYQARIVNHTNTGVLKRLEWLLELMGYMRNIAYQSASAQNVPPAEALDFLMLIFAAAVVAWADHEAPLLLGLSASWLPWHQENGPGGPAAALLGRSPMHRVTVQEVLTLLPTSMLLLLQKEPWKEQTQKFIDWLFSIMEIPNEAFAATSKDLLKATLLSLRVLPEFKKKAVWTRAYGW

Target ORF information:

RefSeq Version	XM_017603024.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus focadhesin (Focad), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017603024.1

ORF Insert Sequence:

ATGTCCGGTT TGATAAAAGC CCTTATGAAA TTATTACAAA TGCAAGCATC CAGGGAAGGA 
CAAGGTGGGG CAAAGGATCT TCAGGACCTA TATTCTATCA GAAATCCTCC TCAGCCTTTG 
ATCACCGTGC TTGAGCACAG GCCTGATTGC TGGCCACTAC TCCTGCAGCA GCTGACAGCT 
TTTTTCCAGC AATGCCCTGA AAGGTCAGAA GCTTCATGTG TCCAAATAAT GGCACCATTT 
CTGTGGTATC TCTACTGTGA ACCATCCCAG TTACAAGAAT ATGCTAAGCT TCGACTGTCC 
CTGCTCAAAG TCTTGCTTCA GCCCAGAGGT GTTTGTGAGG AAGCACAGCC ATCAGTGTTG 
GAACAGCAGA TCCTTCAGCT GTGCTGTAAC ATGGTTCCGT GCTTGCAGAT GAAAGACCTG 
ATGCAGACAA CAGAGATGAC GCTGTTCGTG GAGGAAGTAT ATCTAAGCCT TCTGCGCCAT 
CCTGCTTTCT GGAAGAGACA GCTTACCCAG CTGACCGTTC AGTTGCTGTG CATCTGTGAA 
GTCAGCTTAA AGACAACTGG GGAATGCTCA TCTTTAATTC AGCTTTTAGA GCGCAGTGTT 
GAACTTCTTG GGGAGGATTT TCCTGTCAAA TTGGTCATTA TTGGAATAGC TTTAATACTT 
CTACAGACTC CAGCAAGTCA GCAGAAGCCA GTATTGAACC TAACTCTGAA GCTCCTCTCG 
CTTACTGAGG GTCAGAGAAT CCCGAAGGCC TCTCTGCTGC TCCTGGTGCC TCTTCTGCAG 
ATTCTGTCCT CCACTGCCTT GGAAGACTGT CTGGCCACGG GTGAGGAAGG CCCCTCCAGG 
CAGCAGTTAG CCCTCAGTCT GTTGGAAGTG CTACAGAGAG AGTGTCATGG CGACGACCGA 
CACACGATCT CCTGCAGGCT TGCTTTTCCT GTGAGCAGCA TGTATGGCTC AGTCTTCACT 
GCCTGCAGGA TTCTTGAAGT CATGGGAGAA GCGTCCGCAG CGAGTGACTG GCTGGCTGCA 
GTAGAGTCCT TGCTCCCTGC CACCACAGTG ATCCCCCCAC ATGTTTTCTT ACTGCTGGCT 
CACCTGCTTG TTGAAGATGG AGGACAGAAT CTTCAGCAGA TACTGAAGGT CACCACACAG 
TTAGCTCAAG CAGATTCCTC TCAGGTACCA CATCTGATTC CAGTTCTGAT GTTCAAATTG 
GGGAGACCCT TAGACCCTGT ATCATATAAT CATATATTAT ATACTTTGCC CACACTTGGT 
GTTCACAAGG TATGCGTAGG GCAGATTCTG CGGGTGATTC AACTGCTGGG GACCACACCA 
CGACTAAGAG CTGTCACGTT ACGCCTGTTG ACATCTCTGT GGGAAAAGCA GGATCGTGTG 
TACCCTGAAC TGCAGCGCTT CATGGCCATG TCTGATGCAC CCTCTCTCTC TGTGGGCAAG 
GAGCTGCAGT GGGAGAAACT GATTGCAAAA GCTGCATCCA TCAGAGACAT ATGTAAACAG 
AGGCCCTATC AGCATGGTGC AGACATGCTG GCAGCCATTT CTCAGGTGTT GAACGAATGC 
ACCAAGCCTG ATCAAGCTTC TCCAGCTGCT TTGGTGTTAC AAGGTCTTCA TGCACTCTGC 
CAGGCTGAGG TTGTCTGTAT TCGCTCCACA TGGAATGCTC TCTCTCCCAA GTTGAGCTGT 
GACATGAGAC CACTTATATT GAAGACCCTG AGTGAACTAT TCTCTCTGGT TCCTTCCTTA 
ACAGTCAATA CAGTTGAATA TGAGAATTTT AAAGTTCAAG TCCTCACCTT CCTCTGGACT 
CACACTCAAA ACAAGGATCC CATTGTAGCA AGTGCCGCAT ATAAATCTCT GTCTCACTTC 
AGTGCTGGAG AGCACACCAT TCTTCACCTG CCAGAAAAGA TAAGGCCAGA AATGCCGGTT 
CCCGATGAGT TGGATGAGGA GGAATCTGTG GACCTTTCCA TCCCTGGCTC TTGCTATCTC 
CGACTGCTGA CCATCACTGC TCCTTCAATT TTACCAGCTT TGGAAGAATT CTTCACGTCT 
CTTGTGAGAC AAGAGATGGT GAATATGCCC CGTGGGATAT ACCACTCAGC ATTGAAGGGA 
GGCGTCTGCT CAGACCAAGG GAAGACAGTA GCAGGAATCC CCAACTTTAT ATTGAAGACA 
TATGAAACAA ACAAGCAACC AGGACTGAAA CCCGGTCTGG CAGGTGGCAT GCTGTTTTGC 
TATGATGTGG CCATGTATCA GAGCAAAGAT GGTAAGCCAC TGAATCGGCT GATGGCCAGT 
AGAGGGCGAA CCTTCAAGCA GACAACACTG GCCCTTGTTC ATGAAGTCCA CGTCCAGCTC 
TCAGAATGGC ACCGTGCAAT CTTTCTTCCA CAGGCTTGGC TTGCATACAT GACTCGAGCT 
TACCATGCCA TTTTACAGGG ACGGATAGCA GAGCTGGAGC TGCAGTTGCA ACATGGCAAG 
GAGGGCCCCG AGGAGGTGCA GTACAAACGA AGCACGGCCT GGCTCTGGGT CAGAGACATG 
CTGACAGATG AGATCACCAA GACAGCTGCG AAAGAGAGTC CAGTGGTCAA AGGCAATGCT 
CTGTTGGCCT TAAGCAGCCT TGCCGTCGTG GTGTCCAAGC ATGAAGCCAG CCTTTCCTCA 
GAGTCTGATG GAGTCCTAGA GGTCCAACCT AATTTCCTTC CAGTGAAAGA GTGGGTTTCC 
ATGGTGCTTA ATACTGTCCT GGTCATTGTG GATAGCCATT ACCACCCCAG AGGTCAACTG 
TTCACCTGCT TTTATCATAA GTCCTATTCT GGCGAGAACA CGGCAAGTGC TATTGCGCGC 
TCTGCCGCCG CCACGGCTTT GTCTCTCCTC GTGCCGGTTT TCATTATCTC TTGCAAAGAG 
AAGGTTGAGG AAATCCTGAA CATGCTGACT GCCAGGTTAC CTGGGAAACC AAGTGCTGAT 
GAATCTCAAG CCGTGCAAAT CCACATGGGC CTTGCCTTGG GGATGTTTCT CTCTCGCCTG 
TGTGAGGAGA AACTCAGTGA CATGTCTGGC CAACAGATGA GCCTTCTTCT GATGAAGTCC 
TTGGATGCCC TGGAGAGCTG CTGTTTCGAC CCCAGTCTTG AACACAACAC CGGCTGTATA 
CTGGGAGTTG GACTTGCCCT GTCCCTCATG AGCCACAGCA GCCACACAGA GTCACGCGTT 
CATGTGGCAG CATCCCTGCG GAAGCTGTCT GCCTACCTGG ATGAGAGCGG GAGCCAGAGC 
AGGACATTTC AGGAGGTCCT TGCCTACACA CTTAGCTGTG TGTGTACATC AGCGTTCAGT 
GCTGGCATTA TTGAGGCTGT GGAGGCTGAA GAGACTATGA CCAAGCTCCA GCTGTTGGTG 
GAGAACAACC AGCAGACTTC AGGCTTTGCA CTGGCCTTAG GAAACTTGGT TTATGGGTTG 
TCTGTGTGTG GACATGGAAA AGCTGAAGAC TTGGGCAACA GACTTCGCCC CAGCTGGATT 
AAAATTGTCC TGTCAGAGGG TGCCCCCACC ATGCTCTGTC TGGCAGCCCT TCATGGCCTG 
GTGGCACTGG TAGGCTCCGA TGTGGATGTC ATGCAGTTCC AGTCAGAAGC CATCCACAAC 
TCCCAGTTTC AAGCAAGACT TAATGAAGTC ATTCGAACCT TAACTGAGGT CATCAGTGTC 
TCAGGGGTGA TTGGTCTTCA ATCAAATGCA ATATGGCTGC TAGGACATCT TCATCTATCC 
ACCTTGTCTT CAAATCAAAG CAGAACTTCC GTTCCTACCG ACTACAGCTA TTTGCCTGAA 
GGCAGTTTTA TTAGAGCAGC CATTGGCTTC TTCATCACAG GAGGAAAAAA AGGCCCTGAA 
GCTGTGCCAC CGTCCCTTCT CAAAGTAGTG ATGAAACCCA TAGCAACTGT TGGGGAAAAC 
TACCAGTACC CTCCTGCAAA CTTAGCCGCA CTCCTCTCTC CACTTATGAG GCTAAACTTT 
GGCGAGGAGA TACAGCAACT GTGCCTTGAG ATTGCTGTGT CCCAGGCACC GTCGTCACAG 
AGTGCAGCTG CACTGCTGGG CTTGTGGGTG ATGCCGCCGC TGGTCCACGG TCTGAGTCTG 
AATATCAAGA AATACCTCCT GGTATCTATG CCTCTGTGGG CAAAGCATGT TTCTGATGAA 
CAGATCCAGG GTTTTGTTGA AAATCTGATG GTGGCAGTTT TTAAACCGGC TTCCCAACCT 
TGTCATCCTG AGATGTGCCC AAGTGCCTTA CAGGGTCTGA GCCAGGCCAT GAAACTGCCC 
AGTCCTTCCC ACCACCTCTG GAGTCTGCTC TGCGATGCTA CTGGGAGAAT TTTTGACCTT 
CTGCCAAATA GGATTCGGAG AAATGATCTC GAGCTGTACA TCAGTATAGC AAAGTGCCTC 
TCAGAGATGA CGGATGAAGG TGCCAATCAG GTTTCCCAGA TTACTAAGGA AAACATAGAA 
AAGGCTGCCT TTGTCAAGCT GTACTTAATC TCTCAAGGAC GACTCCCGTT GATGAGCCTG 
ACTGATTTAC TCACTGCTGC TATGCAACAC CCCTCGAAGG AGACTCTAGC CTGGATGATC 
CTGCACAGCT TATACCAGGC ACGCATTGTC AATCACACCA ACACAGGTGT ACTGAAGAGA 
CTGGAGTGGC TCCTGGAGCT GATGGGTTAT ATGAGAAACA TTGCTTACCA GTCGGCGTCT 
GCTCAGAATG TGCCTCCTGC GGAGGCTTTG GACTTCTTGA TGCTGATATT TGCTGCTGCA 
GTGGTAGCAT GGGCAGACCA TGAGGCCCCC CTTCTCCTTG GCCTCAGTGC CAGTTGGTTG 
CCATGGCACC AGGAAAATGG CCCTGGTGGG CCAGCAGCAG CCTTACTTGG CAGGAGTCCG 
ATGCACAGAG TCACTGTGCA GGAGGTTCTC ACTCTCCTCC CCACGAGCAT GCTACTGCTC 
CTGCAAAAAG AGCCATGGAA GGAACAGACC CAGAAGTTCA TTGACTGGCT GTTCAGCATC 
ATGGAAATTC CTAACGAAGC CTTTGCAGCC ACGTCCAAGG ATCTTTTGAA AGCCACCCTG 
CTGTCCTTGA GAGTTCTCCC AGAGTTTAAG AAGAAAGCTG TATGGACCCG AGCGTATGGC 
TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Focad ( XM_017593754.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017449243.1 Rattus norvegicus focadhesin (Focad), transcript variant X1, mRNA.

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CloneID	ORa55473
Clone ID Related Accession (Same CDS sequence)	XM_017603024.1 , XM_017593754.1
Accession Version	XM_017593754.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5106bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	focadhesin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGTT TGATAAAAGC CCTTATGAAA TTATTACAAA TGCAAGCATC CAGGGAAGGA 
CAAGGTGGGG CAAAGGATCT TCAGGACCTA TATTCTATCA GAAATCCTCC TCAGCCTTTG 
ATCACCGTGC TTGAGCACAG GCCTGATTGC TGGCCACTAC TCCTGCAGCA GCTGACAGCT 
TTTTTCCAGC AATGCCCTGA AAGGTCAGAA GCTTCATGTG TCCAAATAAT GGCACCATTT 
CTGTGGTATC TCTACTGTGA ACCATCCCAG TTACAAGAAT ATGCTAAGCT TCGACTGTCC 
CTGCTCAAAG TCTTGCTTCA GCCCAGAGGT GTTTGTGAGG AAGCACAGCC ATCAGTGTTG 
GAACAGCAGA TCCTTCAGCT GTGCTGTAAC ATGGTTCCGT GCTTGCAGAT GAAAGACCTG 
ATGCAGACAA CAGAGATGAC GCTGTTCGTG GAGGAAGTAT ATCTAAGCCT TCTGCGCCAT 
CCTGCTTTCT GGAAGAGACA GCTTACCCAG CTGACCGTTC AGTTGCTGTG CATCTGTGAA 
GTCAGCTTAA AGACAACTGG GGAATGCTCA TCTTTAATTC AGCTTTTAGA GCGCAGTGTT 
GAACTTCTTG GGGAGGATTT TCCTGTCAAA TTGGTCATTA TTGGAATAGC TTTAATACTT 
CTACAGACTC CAGCAAGTCA GCAGAAGCCA GTATTGAACC TAACTCTGAA GCTCCTCTCG 
CTTACTGAGG GTCAGAGAAT CCCGAAGGCC TCTCTGCTGC TCCTGGTGCC TCTTCTGCAG 
ATTCTGTCCT CCACTGCCTT GGAAGACTGT CTGGCCACGG GTGAGGAAGG CCCCTCCAGG 
CAGCAGTTAG CCCTCAGTCT GTTGGAAGTG CTACAGAGAG AGTGTCATGG CGACGACCGA 
CACACGATCT CCTGCAGGCT TGCTTTTCCT GTGAGCAGCA TGTATGGCTC AGTCTTCACT 
GCCTGCAGGA TTCTTGAAGT CATGGGAGAA GCGTCCGCAG CGAGTGACTG GCTGGCTGCA 
GTAGAGTCCT TGCTCCCTGC CACCACAGTG ATCCCCCCAC ATGTTTTCTT ACTGCTGGCT 
CACCTGCTTG TTGAAGATGG AGGACAGAAT CTTCAGCAGA TACTGAAGGT CACCACACAG 
TTAGCTCAAG CAGATTCCTC TCAGGTACCA CATCTGATTC CAGTTCTGAT GTTCAAATTG 
GGGAGACCCT TAGACCCTGT ATCATATAAT CATATATTAT ATACTTTGCC CACACTTGGT 
GTTCACAAGG TATGCGTAGG GCAGATTCTG CGGGTGATTC AACTGCTGGG GACCACACCA 
CGACTAAGAG CTGTCACGTT ACGCCTGTTG ACATCTCTGT GGGAAAAGCA GGATCGTGTG 
TACCCTGAAC TGCAGCGCTT CATGGCCATG TCTGATGCAC CCTCTCTCTC TGTGGGCAAG 
GAGCTGCAGT GGGAGAAACT GATTGCAAAA GCTGCATCCA TCAGAGACAT ATGTAAACAG 
AGGCCCTATC AGCATGGTGC AGACATGCTG GCAGCCATTT CTCAGGTGTT GAACGAATGC 
ACCAAGCCTG ATCAAGCTTC TCCAGCTGCT TTGGTGTTAC AAGGTCTTCA TGCACTCTGC 
CAGGCTGAGG TTGTCTGTAT TCGCTCCACA TGGAATGCTC TCTCTCCCAA GTTGAGCTGT 
GACATGAGAC CACTTATATT GAAGACCCTG AGTGAACTAT TCTCTCTGGT TCCTTCCTTA 
ACAGTCAATA CAGTTGAATA TGAGAATTTT AAAGTTCAAG TCCTCACCTT CCTCTGGACT 
CACACTCAAA ACAAGGATCC CATTGTAGCA AGTGCCGCAT ATAAATCTCT GTCTCACTTC 
AGTGCTGGAG AGCACACCAT TCTTCACCTG CCAGAAAAGA TAAGGCCAGA AATGCCGGTT 
CCCGATGAGT TGGATGAGGA GGAATCTGTG GACCTTTCCA TCCCTGGCTC TTGCTATCTC 
CGACTGCTGA CCATCACTGC TCCTTCAATT TTACCAGCTT TGGAAGAATT CTTCACGTCT 
CTTGTGAGAC AAGAGATGGT GAATATGCCC CGTGGGATAT ACCACTCAGC ATTGAAGGGA 
GGCGTCTGCT CAGACCAAGG GAAGACAGTA GCAGGAATCC CCAACTTTAT ATTGAAGACA 
TATGAAACAA ACAAGCAACC AGGACTGAAA CCCGGTCTGG CAGGTGGCAT GCTGTTTTGC 
TATGATGTGG CCATGTATCA GAGCAAAGAT GGTAAGCCAC TGAATCGGCT GATGGCCAGT 
AGAGGGCGAA CCTTCAAGCA GACAACACTG GCCCTTGTTC ATGAAGTCCA CGTCCAGCTC 
TCAGAATGGC ACCGTGCAAT CTTTCTTCCA CAGGCTTGGC TTGCATACAT GACTCGAGCT 
TACCATGCCA TTTTACAGGG ACGGATAGCA GAGCTGGAGC TGCAGTTGCA ACATGGCAAG 
GAGGGCCCCG AGGAGGTGCA GTACAAACGA AGCACGGCCT GGCTCTGGGT CAGAGACATG 
CTGACAGATG AGATCACCAA GACAGCTGCG AAAGAGAGTC CAGTGGTCAA AGGCAATGCT 
CTGTTGGCCT TAAGCAGCCT TGCCGTCGTG GTGTCCAAGC ATGAAGCCAG CCTTTCCTCA 
GAGTCTGATG GAGTCCTAGA GGTCCAACCT AATTTCCTTC CAGTGAAAGA GTGGGTTTCC 
ATGGTGCTTA ATACTGTCCT GGTCATTGTG GATAGCCATT ACCACCCCAG AGGTCAACTG 
TTCACCTGCT TTTATCATAA GTCCTATTCT GGCGAGAACA CGGCAAGTGC TATTGCGCGC 
TCTGCCGCCG CCACGGCTTT GTCTCTCCTC GTGCCGGTTT TCATTATCTC TTGCAAAGAG 
AAGGTTGAGG AAATCCTGAA CATGCTGACT GCCAGGTTAC CTGGGAAACC AAGTGCTGAT 
GAATCTCAAG CCGTGCAAAT CCACATGGGC CTTGCCTTGG GGATGTTTCT CTCTCGCCTG 
TGTGAGGAGA AACTCAGTGA CATGTCTGGC CAACAGATGA GCCTTCTTCT GATGAAGTCC 
TTGGATGCCC TGGAGAGCTG CTGTTTCGAC CCCAGTCTTG AACACAACAC CGGCTGTATA 
CTGGGAGTTG GACTTGCCCT GTCCCTCATG AGCCACAGCA GCCACACAGA GTCACGCGTT 
CATGTGGCAG CATCCCTGCG GAAGCTGTCT GCCTACCTGG ATGAGAGCGG GAGCCAGAGC 
AGGACATTTC AGGAGGTCCT TGCCTACACA CTTAGCTGTG TGTGTACATC AGCGTTCAGT 
GCTGGCATTA TTGAGGCTGT GGAGGCTGAA GAGACTATGA CCAAGCTCCA GCTGTTGGTG 
GAGAACAACC AGCAGACTTC AGGCTTTGCA CTGGCCTTAG GAAACTTGGT TTATGGGTTG 
TCTGTGTGTG GACATGGAAA AGCTGAAGAC TTGGGCAACA GACTTCGCCC CAGCTGGATT 
AAAATTGTCC TGTCAGAGGG TGCCCCCACC ATGCTCTGTC TGGCAGCCCT TCATGGCCTG 
GTGGCACTGG TAGGCTCCGA TGTGGATGTC ATGCAGTTCC AGTCAGAAGC CATCCACAAC 
TCCCAGTTTC AAGCAAGACT TAATGAAGTC ATTCGAACCT TAACTGAGGT CATCAGTGTC 
TCAGGGGTGA TTGGTCTTCA ATCAAATGCA ATATGGCTGC TAGGACATCT TCATCTATCC 
ACCTTGTCTT CAAATCAAAG CAGAACTTCC GTTCCTACCG ACTACAGCTA TTTGCCTGAA 
GGCAGTTTTA TTAGAGCAGC CATTGGCTTC TTCATCACAG GAGGAAAAAA AGGCCCTGAA 
GCTGTGCCAC CGTCCCTTCT CAAAGTAGTG ATGAAACCCA TAGCAACTGT TGGGGAAAAC 
TACCAGTACC CTCCTGCAAA CTTAGCCGCA CTCCTCTCTC CACTTATGAG GCTAAACTTT 
GGCGAGGAGA TACAGCAACT GTGCCTTGAG ATTGCTGTGT CCCAGGCACC GTCGTCACAG 
AGTGCAGCTG CACTGCTGGG CTTGTGGGTG ATGCCGCCGC TGGTCCACGG TCTGAGTCTG 
AATATCAAGA AATACCTCCT GGTATCTATG CCTCTGTGGG CAAAGCATGT TTCTGATGAA 
CAGATCCAGG GTTTTGTTGA AAATCTGATG GTGGCAGTTT TTAAACCGGC TTCCCAACCT 
TGTCATCCTG AGATGTGCCC AAGTGCCTTA CAGGGTCTGA GCCAGGCCAT GAAACTGCCC 
AGTCCTTCCC ACCACCTCTG GAGTCTGCTC TGCGATGCTA CTGGGAGAAT TTTTGACCTT 
CTGCCAAATA GGATTCGGAG AAATGATCTC GAGCTGTACA TCAGTATAGC AAAGTGCCTC 
TCAGAGATGA CGGATGAAGG TGCCAATCAG GTTTCCCAGA TTACTAAGGA AAACATAGAA 
AAGGCTGCCT TTGTCAAGCT GTACTTAATC TCTCAAGGAC GACTCCCGTT GATGAGCCTG 
ACTGATTTAC TCACTGCTGC TATGCAACAC CCCTCGAAGG AGACTCTAGC CTGGATGATC 
CTGCACAGCT TATACCAGGC ACGCATTGTC AATCACACCA ACACAGGTGT ACTGAAGAGA 
CTGGAGTGGC TCCTGGAGCT GATGGGTTAT ATGAGAAACA TTGCTTACCA GTCGGCGTCT 
GCTCAGAATG TGCCTCCTGC GGAGGCTTTG GACTTCTTGA TGCTGATATT TGCTGCTGCA 
GTGGTAGCAT GGGCAGACCA TGAGGCCCCC CTTCTCCTTG GCCTCAGTGC CAGTTGGTTG 
CCATGGCACC AGGAAAATGG CCCTGGTGGG CCAGCAGCAG CCTTACTTGG CAGGAGTCCG 
ATGCACAGAG TCACTGTGCA GGAGGTTCTC ACTCTCCTCC CCACGAGCAT GCTACTGCTC 
CTGCAAAAAG AGCCATGGAA GGAACAGACC CAGAAGTTCA TTGACTGGCT GTTCAGCATC 
ATGGAAATTC CTAACGAAGC CTTTGCAGCC ACGTCCAAGG ATCTTTTGAA AGCCACCCTG 
CTGTCCTTGA GAGTTCTCCC AGAGTTTAAG AAGAAAGCTG TATGGACCCG AGCGTATGGC 
TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017449243.1
CDS	845..5950
Translation	MSGLIKALMKLLQMQASREGQGGAKDLQDLYSIRNPPQPLITVLEHRPDCWPLLLQQLTAFFQQCPERSEASCVQIMAPFLWYLYCEPSQLQEYAKLRLSLLKVLLQPRGVCEEAQPSVLEQQILQLCCNMVPCLQMKDLMQTTEMTLFVEEVYLSLLRHPAFWKRQLTQLTVQLLCICEVSLKTTGECSSLIQLLERSVELLGEDFPVKLVIIGIALILLQTPASQQKPVLNLTLKLLSLTEGQRIPKASLLLLVPLLQILSSTALEDCLATGEEGPSRQQLALSLLEVLQRECHGDDRHTISCRLAFPVSSMYGSVFTACRILEVMGEASAASDWLAAVESLLPATTVIPPHVFLLLAHLLVEDGGQNLQQILKVTTQLAQADSSQVPHLIPVLMFKLGRPLDPVSYNHILYTLPTLGVHKVCVGQILRVIQLLGTTPRLRAVTLRLLTSLWEKQDRVYPELQRFMAMSDAPSLSVGKELQWEKLIAKAASIRDICKQRPYQHGADMLAAISQVLNECTKPDQASPAALVLQGLHALCQAEVVCIRSTWNALSPKLSCDMRPLILKTLSELFSLVPSLTVNTVEYENFKVQVLTFLWTHTQNKDPIVASAAYKSLSHFSAGEHTILHLPEKIRPEMPVPDELDEEESVDLSIPGSCYLRLLTITAPSILPALEEFFTSLVRQEMVNMPRGIYHSALKGGVCSDQGKTVAGIPNFILKTYETNKQPGLKPGLAGGMLFCYDVAMYQSKDGKPLNRLMASRGRTFKQTTLALVHEVHVQLSEWHRAIFLPQAWLAYMTRAYHAILQGRIAELELQLQHGKEGPEEVQYKRSTAWLWVRDMLTDEITKTAAKESPVVKGNALLALSSLAVVVSKHEASLSSESDGVLEVQPNFLPVKEWVSMVLNTVLVIVDSHYHPRGQLFTCFYHKSYSGENTASAIARSAAATALSLLVPVFIISCKEKVEEILNMLTARLPGKPSADESQAVQIHMGLALGMFLSRLCEEKLSDMSGQQMSLLLMKSLDALESCCFDPSLEHNTGCILGVGLALSLMSHSSHTESRVHVAASLRKLSAYLDESGSQSRTFQEVLAYTLSCVCTSAFSAGIIEAVEAEETMTKLQLLVENNQQTSGFALALGNLVYGLSVCGHGKAEDLGNRLRPSWIKIVLSEGAPTMLCLAALHGLVALVGSDVDVMQFQSEAIHNSQFQARLNEVIRTLTEVISVSGVIGLQSNAIWLLGHLHLSTLSSNQSRTSVPTDYSYLPEGSFIRAAIGFFITGGKKGPEAVPPSLLKVVMKPIATVGENYQYPPANLAALLSPLMRLNFGEEIQQLCLEIAVSQAPSSQSAAALLGLWVMPPLVHGLSLNIKKYLLVSMPLWAKHVSDEQIQGFVENLMVAVFKPASQPCHPEMCPSALQGLSQAMKLPSPSHHLWSLLCDATGRIFDLLPNRIRRNDLELYISIAKCLSEMTDEGANQVSQITKENIEKAAFVKLYLISQGRLPLMSLTDLLTAAMQHPSKETLAWMILHSLYQARIVNHTNTGVLKRLEWLLELMGYMRNIAYQSASAQNVPPAEALDFLMLIFAAAVVAWADHEAPLLLGLSASWLPWHQENGPGGPAAALLGRSPMHRVTVQEVLTLLPTSMLLLLQKEPWKEQTQKFIDWLFSIMEIPNEAFAATSKDLLKATLLSLRVLPEFKKKAVWTRAYGW

Target ORF information:

RefSeq Version	XM_017593754.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus focadhesin (Focad), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017593754.1

ORF Insert Sequence:

ATGTCCGGTT TGATAAAAGC CCTTATGAAA TTATTACAAA TGCAAGCATC CAGGGAAGGA 
CAAGGTGGGG CAAAGGATCT TCAGGACCTA TATTCTATCA GAAATCCTCC TCAGCCTTTG 
ATCACCGTGC TTGAGCACAG GCCTGATTGC TGGCCACTAC TCCTGCAGCA GCTGACAGCT 
TTTTTCCAGC AATGCCCTGA AAGGTCAGAA GCTTCATGTG TCCAAATAAT GGCACCATTT 
CTGTGGTATC TCTACTGTGA ACCATCCCAG TTACAAGAAT ATGCTAAGCT TCGACTGTCC 
CTGCTCAAAG TCTTGCTTCA GCCCAGAGGT GTTTGTGAGG AAGCACAGCC ATCAGTGTTG 
GAACAGCAGA TCCTTCAGCT GTGCTGTAAC ATGGTTCCGT GCTTGCAGAT GAAAGACCTG 
ATGCAGACAA CAGAGATGAC GCTGTTCGTG GAGGAAGTAT ATCTAAGCCT TCTGCGCCAT 
CCTGCTTTCT GGAAGAGACA GCTTACCCAG CTGACCGTTC AGTTGCTGTG CATCTGTGAA 
GTCAGCTTAA AGACAACTGG GGAATGCTCA TCTTTAATTC AGCTTTTAGA GCGCAGTGTT 
GAACTTCTTG GGGAGGATTT TCCTGTCAAA TTGGTCATTA TTGGAATAGC TTTAATACTT 
CTACAGACTC CAGCAAGTCA GCAGAAGCCA GTATTGAACC TAACTCTGAA GCTCCTCTCG 
CTTACTGAGG GTCAGAGAAT CCCGAAGGCC TCTCTGCTGC TCCTGGTGCC TCTTCTGCAG 
ATTCTGTCCT CCACTGCCTT GGAAGACTGT CTGGCCACGG GTGAGGAAGG CCCCTCCAGG 
CAGCAGTTAG CCCTCAGTCT GTTGGAAGTG CTACAGAGAG AGTGTCATGG CGACGACCGA 
CACACGATCT CCTGCAGGCT TGCTTTTCCT GTGAGCAGCA TGTATGGCTC AGTCTTCACT 
GCCTGCAGGA TTCTTGAAGT CATGGGAGAA GCGTCCGCAG CGAGTGACTG GCTGGCTGCA 
GTAGAGTCCT TGCTCCCTGC CACCACAGTG ATCCCCCCAC ATGTTTTCTT ACTGCTGGCT 
CACCTGCTTG TTGAAGATGG AGGACAGAAT CTTCAGCAGA TACTGAAGGT CACCACACAG 
TTAGCTCAAG CAGATTCCTC TCAGGTACCA CATCTGATTC CAGTTCTGAT GTTCAAATTG 
GGGAGACCCT TAGACCCTGT ATCATATAAT CATATATTAT ATACTTTGCC CACACTTGGT 
GTTCACAAGG TATGCGTAGG GCAGATTCTG CGGGTGATTC AACTGCTGGG GACCACACCA 
CGACTAAGAG CTGTCACGTT ACGCCTGTTG ACATCTCTGT GGGAAAAGCA GGATCGTGTG 
TACCCTGAAC TGCAGCGCTT CATGGCCATG TCTGATGCAC CCTCTCTCTC TGTGGGCAAG 
GAGCTGCAGT GGGAGAAACT GATTGCAAAA GCTGCATCCA TCAGAGACAT ATGTAAACAG 
AGGCCCTATC AGCATGGTGC AGACATGCTG GCAGCCATTT CTCAGGTGTT GAACGAATGC 
ACCAAGCCTG ATCAAGCTTC TCCAGCTGCT TTGGTGTTAC AAGGTCTTCA TGCACTCTGC 
CAGGCTGAGG TTGTCTGTAT TCGCTCCACA TGGAATGCTC TCTCTCCCAA GTTGAGCTGT 
GACATGAGAC CACTTATATT GAAGACCCTG AGTGAACTAT TCTCTCTGGT TCCTTCCTTA 
ACAGTCAATA CAGTTGAATA TGAGAATTTT AAAGTTCAAG TCCTCACCTT CCTCTGGACT 
CACACTCAAA ACAAGGATCC CATTGTAGCA AGTGCCGCAT ATAAATCTCT GTCTCACTTC 
AGTGCTGGAG AGCACACCAT TCTTCACCTG CCAGAAAAGA TAAGGCCAGA AATGCCGGTT 
CCCGATGAGT TGGATGAGGA GGAATCTGTG GACCTTTCCA TCCCTGGCTC TTGCTATCTC 
CGACTGCTGA CCATCACTGC TCCTTCAATT TTACCAGCTT TGGAAGAATT CTTCACGTCT 
CTTGTGAGAC AAGAGATGGT GAATATGCCC CGTGGGATAT ACCACTCAGC ATTGAAGGGA 
GGCGTCTGCT CAGACCAAGG GAAGACAGTA GCAGGAATCC CCAACTTTAT ATTGAAGACA 
TATGAAACAA ACAAGCAACC AGGACTGAAA CCCGGTCTGG CAGGTGGCAT GCTGTTTTGC 
TATGATGTGG CCATGTATCA GAGCAAAGAT GGTAAGCCAC TGAATCGGCT GATGGCCAGT 
AGAGGGCGAA CCTTCAAGCA GACAACACTG GCCCTTGTTC ATGAAGTCCA CGTCCAGCTC 
TCAGAATGGC ACCGTGCAAT CTTTCTTCCA CAGGCTTGGC TTGCATACAT GACTCGAGCT 
TACCATGCCA TTTTACAGGG ACGGATAGCA GAGCTGGAGC TGCAGTTGCA ACATGGCAAG 
GAGGGCCCCG AGGAGGTGCA GTACAAACGA AGCACGGCCT GGCTCTGGGT CAGAGACATG 
CTGACAGATG AGATCACCAA GACAGCTGCG AAAGAGAGTC CAGTGGTCAA AGGCAATGCT 
CTGTTGGCCT TAAGCAGCCT TGCCGTCGTG GTGTCCAAGC ATGAAGCCAG CCTTTCCTCA 
GAGTCTGATG GAGTCCTAGA GGTCCAACCT AATTTCCTTC CAGTGAAAGA GTGGGTTTCC 
ATGGTGCTTA ATACTGTCCT GGTCATTGTG GATAGCCATT ACCACCCCAG AGGTCAACTG 
TTCACCTGCT TTTATCATAA GTCCTATTCT GGCGAGAACA CGGCAAGTGC TATTGCGCGC 
TCTGCCGCCG CCACGGCTTT GTCTCTCCTC GTGCCGGTTT TCATTATCTC TTGCAAAGAG 
AAGGTTGAGG AAATCCTGAA CATGCTGACT GCCAGGTTAC CTGGGAAACC AAGTGCTGAT 
GAATCTCAAG CCGTGCAAAT CCACATGGGC CTTGCCTTGG GGATGTTTCT CTCTCGCCTG 
TGTGAGGAGA AACTCAGTGA CATGTCTGGC CAACAGATGA GCCTTCTTCT GATGAAGTCC 
TTGGATGCCC TGGAGAGCTG CTGTTTCGAC CCCAGTCTTG AACACAACAC CGGCTGTATA 
CTGGGAGTTG GACTTGCCCT GTCCCTCATG AGCCACAGCA GCCACACAGA GTCACGCGTT 
CATGTGGCAG CATCCCTGCG GAAGCTGTCT GCCTACCTGG ATGAGAGCGG GAGCCAGAGC 
AGGACATTTC AGGAGGTCCT TGCCTACACA CTTAGCTGTG TGTGTACATC AGCGTTCAGT 
GCTGGCATTA TTGAGGCTGT GGAGGCTGAA GAGACTATGA CCAAGCTCCA GCTGTTGGTG 
GAGAACAACC AGCAGACTTC AGGCTTTGCA CTGGCCTTAG GAAACTTGGT TTATGGGTTG 
TCTGTGTGTG GACATGGAAA AGCTGAAGAC TTGGGCAACA GACTTCGCCC CAGCTGGATT 
AAAATTGTCC TGTCAGAGGG TGCCCCCACC ATGCTCTGTC TGGCAGCCCT TCATGGCCTG 
GTGGCACTGG TAGGCTCCGA TGTGGATGTC ATGCAGTTCC AGTCAGAAGC CATCCACAAC 
TCCCAGTTTC AAGCAAGACT TAATGAAGTC ATTCGAACCT TAACTGAGGT CATCAGTGTC 
TCAGGGGTGA TTGGTCTTCA ATCAAATGCA ATATGGCTGC TAGGACATCT TCATCTATCC 
ACCTTGTCTT CAAATCAAAG CAGAACTTCC GTTCCTACCG ACTACAGCTA TTTGCCTGAA 
GGCAGTTTTA TTAGAGCAGC CATTGGCTTC TTCATCACAG GAGGAAAAAA AGGCCCTGAA 
GCTGTGCCAC CGTCCCTTCT CAAAGTAGTG ATGAAACCCA TAGCAACTGT TGGGGAAAAC 
TACCAGTACC CTCCTGCAAA CTTAGCCGCA CTCCTCTCTC CACTTATGAG GCTAAACTTT 
GGCGAGGAGA TACAGCAACT GTGCCTTGAG ATTGCTGTGT CCCAGGCACC GTCGTCACAG 
AGTGCAGCTG CACTGCTGGG CTTGTGGGTG ATGCCGCCGC TGGTCCACGG TCTGAGTCTG 
AATATCAAGA AATACCTCCT GGTATCTATG CCTCTGTGGG CAAAGCATGT TTCTGATGAA 
CAGATCCAGG GTTTTGTTGA AAATCTGATG GTGGCAGTTT TTAAACCGGC TTCCCAACCT 
TGTCATCCTG AGATGTGCCC AAGTGCCTTA CAGGGTCTGA GCCAGGCCAT GAAACTGCCC 
AGTCCTTCCC ACCACCTCTG GAGTCTGCTC TGCGATGCTA CTGGGAGAAT TTTTGACCTT 
CTGCCAAATA GGATTCGGAG AAATGATCTC GAGCTGTACA TCAGTATAGC AAAGTGCCTC 
TCAGAGATGA CGGATGAAGG TGCCAATCAG GTTTCCCAGA TTACTAAGGA AAACATAGAA 
AAGGCTGCCT TTGTCAAGCT GTACTTAATC TCTCAAGGAC GACTCCCGTT GATGAGCCTG 
ACTGATTTAC TCACTGCTGC TATGCAACAC CCCTCGAAGG AGACTCTAGC CTGGATGATC 
CTGCACAGCT TATACCAGGC ACGCATTGTC AATCACACCA ACACAGGTGT ACTGAAGAGA 
CTGGAGTGGC TCCTGGAGCT GATGGGTTAT ATGAGAAACA TTGCTTACCA GTCGGCGTCT 
GCTCAGAATG TGCCTCCTGC GGAGGCTTTG GACTTCTTGA TGCTGATATT TGCTGCTGCA 
GTGGTAGCAT GGGCAGACCA TGAGGCCCCC CTTCTCCTTG GCCTCAGTGC CAGTTGGTTG 
CCATGGCACC AGGAAAATGG CCCTGGTGGG CCAGCAGCAG CCTTACTTGG CAGGAGTCCG 
ATGCACAGAG TCACTGTGCA GGAGGTTCTC ACTCTCCTCC CCACGAGCAT GCTACTGCTC 
CTGCAAAAAG AGCCATGGAA GGAACAGACC CAGAAGTTCA TTGACTGGCT GTTCAGCATC 
ATGGAAATTC CTAACGAAGC CTTTGCAGCC ACGTCCAAGG ATCTTTTGAA AGCCACCCTG 
CTGTCCTTGA GAGTTCTCCC AGAGTTTAAG AAGAAAGCTG TATGGACCCG AGCGTATGGC 
TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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Focad cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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