Pex5 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pex5
Entrez Gene ID	312703
Full Name	peroxisomal biogenesis factor 5
Synonyms	PTS1-BP,PTS1R
General protein information	Preferred Names peroxisomal biogenesis factor 5 Names peroxisomal targeting signal 1 receptor PTS1 receptor peroxin-5 peroxisomal C-terminal targeting signal import receptor peroxisome biogenesis factor 5 peroxisome receptor 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 4 Map Location: 4q42

mRNA and Protein(s)

mRNA	Protein	Name
XM_006237385.3	XP_006237447.1	peroxisomal targeting signal 1 receptor isoform X1
NM_001170584.1	NP_001164055.1	peroxisomal targeting signal 1 receptor
XM_006237384.2	XP_006237446.1	peroxisomal targeting signal 1 receptor isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	PEX5	NP_001039648.1
Mus musculus (house mouse)	Pex5	NP_001264259.1
Gallus gallus (chicken)	PEX5	NP_001012836.1
Danio rerio (zebrafish)	pex5	NP_957450.1
Homo sapiens (human)	PEX5	NP_001124498.1
Pan troglodytes (chimpanzee)	PEX5	XP_001164247.2
Xenopus tropicalis (tropical clawed frog)	pex5	NP_001011381.1
Macaca mulatta (Rhesus monkey)	PEX5	XP_002798517.1
Canis lupus familiaris (dog)	PEX5	XP_005637261.1
Rattus norvegicus (Norway rat)	Pex5	XP_006237446.1
Drosophila melanogaster (fruit fly)	Pex5	NP_569949.3
Caenorhabditis elegans (roundworm)	prx-5	NP_001022020.1

Gene Ontology
Bibliography

Related articles in PubMed

Identification of ubiquitin-specific protease 9X (USP9X) as a deubiquitinase acting on ubiquitin-peroxin 5 (PEX5) thioester conjugate.
Grou CP, Francisco T, Rodrigues TA, Freitas MO, Pinto MP, Carvalho AF, Domingues P, Wood SA, Rodríguez-Borges JE, Sá-Miranda C, Fransen M, Azevedo JE
The Journal of biological chemistry287(16)12815-27(2012 Apr)

PEX5 protein binds monomeric catalase blocking its tetramerization and releases it upon binding the N-terminal domain of PEX14.
Freitas MO, Francisco T, Rodrigues TA, Alencastre IS, Pinto MP, Grou CP, Carvalho AF, Fransen M, Sá-Miranda C, Azevedo JE
The Journal of biological chemistry286(47)40509-19(2011 Nov)

PEX14 is required for microtubule-based peroxisome motility in human cells.
Bharti P, Schliebs W, Schievelbusch T, Neuhaus A, David C, Kock K, Herrmann C, Meyer HE, Wiese S, Warscheid B, Theiss C, Erdmann R
Journal of cell science124(Pt 10)1759-68(2011 May)

The Peroxisomal Targeting Signal 1 in sterol carrier protein 2 is autonomous and essential for receptor recognition.
Williams CP, Schueller N, Thompson CA, van den Berg M, Van Haren SD, Erdmann R, Bond CS, Distel B, Schliebs W, Wilmanns M, Stanley WA
BMC biochemistry1212(2011 Mar)

A proteome-wide perspective on peroxisome targeting signal 1(PTS1)-Pex5p affinities.
Ghosh D, Berg JM
Journal of the American Chemical Society132(11)3973-9(2010 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Pex5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pex5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa06350	NM_001170584.1 Latest version!	Rattus norvegicus peroxisomal biogenesis factor 5 (Pex5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ORa27779	XM_006237385.3 Latest version!	Rattus norvegicus peroxisomal biogenesis factor 5 (Pex5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ORa27779	XM_006237384.2 Latest version!	Rattus norvegicus peroxisomal biogenesis factor 5 (Pex5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pex5 ( NM_001170584.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001164055.1 Rattus norvegicus peroxisomal biogenesis factor 5 (Pex5), mRNA.

Price & Availability↑

CloneID	ORa06350
Clone ID Related Accession (Same CDS sequence)	NM_001170584.1
Accession Version	NM_001170584.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1568995200000
Organism	Rattus norvegicus(Norway rat)
Product	peroxisomal targeting signal 1 receptor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from FM036217.1, CK477113.1, CB809540.1, CK469240.1, EV779934.1 and BC111709.1. On or before Dec 15, 2013 this sequence version replaced XM_006237386.1, XM_232343.5, XM_001062081.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAATGC GGGAGCTGGT GGAGAGCGAA TGTGGGGGTG CCAACCCGCT GATGAAGCTG 
GCCACCCACT TCACCCAGGA CAAGGCCCTT CGGCAGGAGG GACTGAGGCC TGGCCCCTGG 
CCTCCAGGAG CCTCAGCGGC GGAAACGGTT TCCAAGCCTT TGGGAGTAGG CACTGAAGAT 
GAGTTGGTGG CAGAATTCCT GCAGGACCAG AATGCAACTC TTGTATCCCG AGCCCCTCAG 
ACCTTCAAGA TGGATGACCT CTTGGCGGAG ATGCAGGAGA TTGAACAGTC AAACTTTCGC 
CAGGCTCCTC AGAGAGCCCC TGGTGTGGCA GACTTGGCTT TGTCTGAGAA TTGGGCCCAG 
GAGTTTCTTG CCGCTGGAGA TGCTGTGGAT GTAGCTCAGG ATTATAACGA GACCGACTGG 
TCCCAAGAAT TCATCGCCGA AGTCACGGAC CCTCTGTCTG TGTCCCCAGC CCGATGGGCT 
GAGGAGTATC TGGAACAGTC TGAGGAGAAG CTGTGGTTGG GAGATCCAGA AGGAACCTCT 
ACCACTGACC GATGGTACGA TGAATACCAT CCTGAGGAGG ATCTGCAGCA CACAGCCAGT 
GACTTTGTGT CCAAGGTGGA CGACCCCAAA TTGGCTAACT CTGAGGGCAC ATCGGAGGCC 
TGGGTTGATC AGTTCACAAG ACCAGGAAAC AAACTGGCTG CTCTTCAGGT GGAATTTGAA 
CGAGCCAAGT CAGCTATAGA GTCAGACGTC GATTTCTGGG ACAAGTTGCA GGCAGAGTTG 
GAGGAGATGG CAAAACGGGA TGCTGAGGCG CACCCCTGGC TTTCTGACTA TGACGACCTC 
ACGTCTGCTT CCTATGACAA GGGCTACCAG TTTGAGGAGG AGAATCCCTT GCGTGACCAC 
CCTCAGCCTT TTGAAGAAGG GCTGCGTCGA CTTGAGGAGG GAGACCTGCC CAATGCTGTG 
CTGCTCTTCG AGGCTGCGGT GCAGCAGGAT CCTAAGCACA TGGAGGCCTG GCAGTACCTG 
GGCACCACTC AGGCTGAGAA CGAGCAAGAG CTCTTGGCCA TCAGTGCCCT GCGCAGGTGT 
CTGGAGCTAA AGCCAGATAA CCGGACAGCA CTGATGGCGC TGGCTGTAAG CTTCACCAAT 
GAGTCCCTGC AGCGGCAAGC CTGTGAGACG CTGCGGGACT GGCTGCGCTA CTCACCGGCC 
TACGCTCACC TGGTGGCTCC TGGTGAAGAA GGGGCTAGTG GGGCAGGACT GGGTCCCAGC 
AAGCGCGTCT TGGGATCTCT GTTGTCTGAC TCCCTGTTTC TTGAAGTAAA AGAACTCTTC 
CTGGCAGCTG TGCGTCTGGA CCCTACATCC ATTGACCCTG ACGTGCAGTG TGGCCTGGGA 
GTCCTCTTCA ACCTGAGTGG GGAGTACGAC AAGGCTGTGG ACTGTTTCAC GGCTGCCCTC 
AGTGTCCGCC CCAATGACTA TTTGCTGTGG AACAAGCTTG GGGCCACCCT GGCCAACGGG 
AACCAGAGTG AAGAAGCAGT GGCTGCTTAC CGCAGAGCCC TTGAACTACA GCCTGGGTAC 
ATCCGCTCCC GCTACAACCT AGGCATCAGC TGCATCAACC TTGGGGCTCA CCGTGAGGCC 
GTGGAACACT TTCTGGAGGC CCTGAACATG CAGAGGAAGA GCCGGGGCCC TCGGGGTGAG 
GGGGGCGCCA TGTCTGAGAA CATCTGGAGT ACTCTGCGGT TGGCATTGTC CATGCTAGGC 
CAGAGTGATG CCTATGGGGC AGCCGATGCT CGGGACCTGC CTGCCCTTCT AACTATGTTT 
GGCCTGCCCC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001164055.1
CDS	207..2021
Translation	MAMRELVESECGGANPLMKLATHFTQDKALRQEGLRPGPWPPGASAAETVSKPLGVGTEDELVAEFLQDQNATLVSRAPQTFKMDDLLAEMQEIEQSNFRQAPQRAPGVADLALSENWAQEFLAAGDAVDVAQDYNETDWSQEFIAEVTDPLSVSPARWAEEYLEQSEEKLWLGDPEGTSTTDRWYDEYHPEEDLQHTASDFVSKVDDPKLANSEGTSEAWVDQFTRPGNKLAALQVEFERAKSAIESDVDFWDKLQAELEEMAKRDAEAHPWLSDYDDLTSASYDKGYQFEEENPLRDHPQPFEEGLRRLEEGDLPNAVLLFEAAVQQDPKHMEAWQYLGTTQAENEQELLAISALRRCLELKPDNRTALMALAVSFTNESLQRQACETLRDWLRYSPAYAHLVAPGEEGASGAGLGPSKRVLGSLLSDSLFLEVKELFLAAVRLDPTSIDPDVQCGLGVLFNLSGEYDKAVDCFTAALSVRPNDYLLWNKLGATLANGNQSEEAVAAYRRALELQPGYIRSRYNLGISCINLGAHREAVEHFLEALNMQRKSRGPRGEGGAMSENIWSTLRLALSMLGQSDAYGAADARDLPALLTMFGLPQ

Target ORF information:

RefSeq Version	NM_001170584.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus peroxisomal biogenesis factor 5 (Pex5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170584.1

ORF Insert Sequence:

ATGGCAATGC GGGAGCTGGT GGAGAGCGAA TGTGGGGGTG CCAACCCGCT GATGAAGCTG 
GCCACCCACT TCACCCAGGA CAAGGCCCTT CGGCAGGAGG GACTGAGGCC TGGCCCCTGG 
CCTCCAGGAG CCTCAGCGGC GGAAACGGTT TCCAAGCCTT TGGGAGTAGG CACTGAAGAT 
GAGTTGGTGG CAGAATTCCT GCAGGACCAG AATGCAACTC TTGTATCCCG AGCCCCTCAG 
ACCTTCAAGA TGGATGACCT CTTGGCGGAG ATGCAGGAGA TTGAACAGTC AAACTTTCGC 
CAGGCTCCTC AGAGAGCCCC TGGTGTGGCA GACTTGGCTT TGTCTGAGAA TTGGGCCCAG 
GAGTTTCTTG CCGCTGGAGA TGCTGTGGAT GTAGCTCAGG ATTATAACGA GACCGACTGG 
TCCCAAGAAT TCATCGCCGA AGTCACGGAC CCTCTGTCTG TGTCCCCAGC CCGATGGGCT 
GAGGAGTATC TGGAACAGTC TGAGGAGAAG CTGTGGTTGG GAGATCCAGA AGGAACCTCT 
ACCACTGACC GATGGTACGA TGAATACCAT CCTGAGGAGG ATCTGCAGCA CACAGCCAGT 
GACTTTGTGT CCAAGGTGGA CGACCCCAAA TTGGCTAACT CTGAGGGCAC ATCGGAGGCC 
TGGGTTGATC AGTTCACAAG ACCAGGAAAC AAACTGGCTG CTCTTCAGGT GGAATTTGAA 
CGAGCCAAGT CAGCTATAGA GTCAGACGTC GATTTCTGGG ACAAGTTGCA GGCAGAGTTG 
GAGGAGATGG CAAAACGGGA TGCTGAGGCG CACCCCTGGC TTTCTGACTA TGACGACCTC 
ACGTCTGCTT CCTATGACAA GGGCTACCAG TTTGAGGAGG AGAATCCCTT GCGTGACCAC 
CCTCAGCCTT TTGAAGAAGG GCTGCGTCGA CTTGAGGAGG GAGACCTGCC CAATGCTGTG 
CTGCTCTTCG AGGCTGCGGT GCAGCAGGAT CCTAAGCACA TGGAGGCCTG GCAGTACCTG 
GGCACCACTC AGGCTGAGAA CGAGCAAGAG CTCTTGGCCA TCAGTGCCCT GCGCAGGTGT 
CTGGAGCTAA AGCCAGATAA CCGGACAGCA CTGATGGCGC TGGCTGTAAG CTTCACCAAT 
GAGTCCCTGC AGCGGCAAGC CTGTGAGACG CTGCGGGACT GGCTGCGCTA CTCACCGGCC 
TACGCTCACC TGGTGGCTCC TGGTGAAGAA GGGGCTAGTG GGGCAGGACT GGGTCCCAGC 
AAGCGCGTCT TGGGATCTCT GTTGTCTGAC TCCCTGTTTC TTGAAGTAAA AGAACTCTTC 
CTGGCAGCTG TGCGTCTGGA CCCTACATCC ATTGACCCTG ACGTGCAGTG TGGCCTGGGA 
GTCCTCTTCA ACCTGAGTGG GGAGTACGAC AAGGCTGTGG ACTGTTTCAC GGCTGCCCTC 
AGTGTCCGCC CCAATGACTA TTTGCTGTGG AACAAGCTTG GGGCCACCCT GGCCAACGGG 
AACCAGAGTG AAGAAGCAGT GGCTGCTTAC CGCAGAGCCC TTGAACTACA GCCTGGGTAC 
ATCCGCTCCC GCTACAACCT AGGCATCAGC TGCATCAACC TTGGGGCTCA CCGTGAGGCC 
GTGGAACACT TTCTGGAGGC CCTGAACATG CAGAGGAAGA GCCGGGGCCC TCGGGGTGAG 
GGGGGCGCCA TGTCTGAGAA CATCTGGAGT ACTCTGCGGT TGGCATTGTC CATGCTAGGC 
CAGAGTGATG CCTATGGGGC AGCCGATGCT CGGGACCTGC CTGCCCTTCT AACTATGTTT 
GGCCTGCCCC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pex5 ( XM_006237385.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006237447.1 Rattus norvegicus peroxisomal biogenesis factor 5 (Pex5), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa27779
Clone ID Related Accession (Same CDS sequence)	XM_006237385.3 , XM_006237384.2
Accession Version	XM_006237385.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1926bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	peroxisomal targeting signal 1 receptor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006237385.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAATGC GGGAGCTGGT GGAGAGCGAA TGTGGGGGTG CCAACCCGCT GATGAAGCTG 
GCCACCCACT TCACCCAGGA CAAGGCCCTT CGGCAGGAGG GACTGAGGCC TGGCCCCTGG 
CCTCCAGGAG CCTCAGCGGC GGAAACGGTT TCCAAGCCTT TGGGAGTAGG CACTGAAGAT 
GAGTTGGTGG CAGAATTCCT GCAGGACCAG AATGCAACTC TTGTATCCCG AGCCCCTCAG 
ACCTTCAAGA TGGATGACCT CTTGGCGGAG ATGCAGGAGA TTGAACAGTC AAACTTTCGC 
CAGGCTCCTC AGAGAGCCCC TGGTGTGGCA GACTTGGCTT TGTCTGAGAA TTGGGCCCAG 
GAGTTTCTTG CCGCTGGAGA TGCTGTGGAT GTAGCTCAGG ATTATAACGA GACCGACTGG 
TCCCAAGAAT TCATCGCCGA AGTCACGGAC CCTCTGTCTG TGTCCCCAGC CCGATGGGCT 
GAGGAGTATC TGGAACAGTC TGAGGAGAAG CTGTGGTTGG GAGATCCAGA AGGAACCTCT 
ACCACTGACC GATGGTACGA TGAATACCAT CCTGAGGAGG ATCTGCAGCA CACAGCCAGT 
GACTTTGTGT CCAAGGTGGA CGACCCCAAA TTGGCTAACT CTGAGTTCCT GAAATTCGTG 
CGGCAGATTG GCGAGGGGCA GGTGTCCCTG GAGTCCGCTG CAGGGTCGGG CCGAGCTCAG 
GCAGAACAGT GGGCAGCAGA GTTTATACAG CAGCAGGGCA CATCGGAGGC CTGGGTTGAT 
CAGTTCACAA GACCAGGAAA CAAACTGGCT GCTCTTCAGG TGGAATTTGA ACGAGCCAAG 
TCAGCTATAG AGTCAGACGT CGATTTCTGG GACAAGTTGC AGGCAGAGTT GGAGGAGATG 
GCAAAACGGG ATGCTGAGGC GCACCCCTGG CTTTCTGACT ATGACGACCT CACGTCTGCT 
TCCTATGACA AGGGCTACCA GTTTGAGGAG GAGAATCCCT TGCGTGACCA CCCTCAGCCT 
TTTGAAGAAG GGCTGCGTCG ACTTGAGGAG GGAGACCTGC CCAATGCTGT GCTGCTCTTC 
GAGGCTGCGG TGCAGCAGGA TCCTAAGCAC ATGGAGGCCT GGCAGTACCT GGGCACCACT 
CAGGCTGAGA ACGAGCAAGA GCTCTTGGCC ATCAGTGCCC TGCGCAGGTG TCTGGAGCTA 
AAGCCAGATA ACCGGACAGC ACTGATGGCG CTGGCTGTAA GCTTCACCAA TGAGTCCCTG 
CAGCGGCAAG CCTGTGAGAC GCTGCGGGAC TGGCTGCGCT ACTCACCGGC CTACGCTCAC 
CTGGTGGCTC CTGGTGAAGA AGGGGCTAGT GGGGCAGGAC TGGGTCCCAG CAAGCGCGTC 
TTGGGATCTC TGTTGTCTGA CTCCCTGTTT CTTGAAGTAA AAGAACTCTT CCTGGCAGCT 
GTGCGTCTGG ACCCTACATC CATTGACCCT GACGTGCAGT GTGGCCTGGG AGTCCTCTTC 
AACCTGAGTG GGGAGTACGA CAAGGCTGTG GACTGTTTCA CGGCTGCCCT CAGTGTCCGC 
CCCAATGACT ATTTGCTGTG GAACAAGCTT GGGGCCACCC TGGCCAACGG GAACCAGAGT 
GAAGAAGCAG TGGCTGCTTA CCGCAGAGCC CTTGAACTAC AGCCTGGGTA CATCCGCTCC 
CGCTACAACC TAGGCATCAG CTGCATCAAC CTTGGGGCTC ACCGTGAGGC CGTGGAACAC 
TTTCTGGAGG CCCTGAACAT GCAGAGGAAG AGCCGGGGCC CTCGGGGTGA GGGGGGCGCC 
ATGTCTGAGA ACATCTGGAG TACTCTGCGG TTGGCATTGT CCATGCTAGG CCAGAGTGAT 
GCCTATGGGG CAGCCGATGC TCGGGACCTG CCTGCCCTTC TAACTATGTT TGGCCTGCCC 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006237447.1
CDS	142..2067
Translation	MAMRELVESECGGANPLMKLATHFTQDKALRQEGLRPGPWPPGASAAETVSKPLGVGTEDELVAEFLQDQNATLVSRAPQTFKMDDLLAEMQEIEQSNFRQAPQRAPGVADLALSENWAQEFLAAGDAVDVAQDYNETDWSQEFIAEVTDPLSVSPARWAEEYLEQSEEKLWLGDPEGTSTTDRWYDEYHPEEDLQHTASDFVSKVDDPKLANSEFLKFVRQIGEGQVSLESAAGSGRAQAEQWAAEFIQQQGTSEAWVDQFTRPGNKLAALQVEFERAKSAIESDVDFWDKLQAELEEMAKRDAEAHPWLSDYDDLTSASYDKGYQFEEENPLRDHPQPFEEGLRRLEEGDLPNAVLLFEAAVQQDPKHMEAWQYLGTTQAENEQELLAISALRRCLELKPDNRTALMALAVSFTNESLQRQACETLRDWLRYSPAYAHLVAPGEEGASGAGLGPSKRVLGSLLSDSLFLEVKELFLAAVRLDPTSIDPDVQCGLGVLFNLSGEYDKAVDCFTAALSVRPNDYLLWNKLGATLANGNQSEEAVAAYRRALELQPGYIRSRYNLGISCINLGAHREAVEHFLEALNMQRKSRGPRGEGGAMSENIWSTLRLALSMLGQSDAYGAADARDLPALLTMFGLPQ

Target ORF information:

RefSeq Version	XM_006237385.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus peroxisomal biogenesis factor 5 (Pex5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006237385.3

ORF Insert Sequence:

ATGGCAATGC GGGAGCTGGT GGAGAGCGAA TGTGGGGGTG CCAACCCGCT GATGAAGCTG 
GCCACCCACT TCACCCAGGA CAAGGCCCTT CGGCAGGAGG GACTGAGGCC TGGCCCCTGG 
CCTCCAGGAG CCTCAGCGGC GGAAACGGTT TCCAAGCCTT TGGGAGTAGG CACTGAAGAT 
GAGTTGGTGG CAGAATTCCT GCAGGACCAG AATGCAACTC TTGTATCCCG AGCCCCTCAG 
ACCTTCAAGA TGGATGACCT CTTGGCGGAG ATGCAGGAGA TTGAACAGTC AAACTTTCGC 
CAGGCTCCTC AGAGAGCCCC TGGTGTGGCA GACTTGGCTT TGTCTGAGAA TTGGGCCCAG 
GAGTTTCTTG CCGCTGGAGA TGCTGTGGAT GTAGCTCAGG ATTATAACGA GACCGACTGG 
TCCCAAGAAT TCATCGCCGA AGTCACGGAC CCTCTGTCTG TGTCCCCAGC CCGATGGGCT 
GAGGAGTATC TGGAACAGTC TGAGGAGAAG CTGTGGTTGG GAGATCCAGA AGGAACCTCT 
ACCACTGACC GATGGTACGA TGAATACCAT CCTGAGGAGG ATCTGCAGCA CACAGCCAGT 
GACTTTGTGT CCAAGGTGGA CGACCCCAAA TTGGCTAACT CTGAGTTCCT GAAATTCGTG 
CGGCAGATTG GCGAGGGGCA GGTGTCCCTG GAGTCCGCTG CAGGGTCGGG CCGAGCTCAG 
GCAGAACAGT GGGCAGCAGA GTTTATACAG CAGCAGGGCA CATCGGAGGC CTGGGTTGAT 
CAGTTCACAA GACCAGGAAA CAAACTGGCT GCTCTTCAGG TGGAATTTGA ACGAGCCAAG 
TCAGCTATAG AGTCAGACGT CGATTTCTGG GACAAGTTGC AGGCAGAGTT GGAGGAGATG 
GCAAAACGGG ATGCTGAGGC GCACCCCTGG CTTTCTGACT ATGACGACCT CACGTCTGCT 
TCCTATGACA AGGGCTACCA GTTTGAGGAG GAGAATCCCT TGCGTGACCA CCCTCAGCCT 
TTTGAAGAAG GGCTGCGTCG ACTTGAGGAG GGAGACCTGC CCAATGCTGT GCTGCTCTTC 
GAGGCTGCGG TGCAGCAGGA TCCTAAGCAC ATGGAGGCCT GGCAGTACCT GGGCACCACT 
CAGGCTGAGA ACGAGCAAGA GCTCTTGGCC ATCAGTGCCC TGCGCAGGTG TCTGGAGCTA 
AAGCCAGATA ACCGGACAGC ACTGATGGCG CTGGCTGTAA GCTTCACCAA TGAGTCCCTG 
CAGCGGCAAG CCTGTGAGAC GCTGCGGGAC TGGCTGCGCT ACTCACCGGC CTACGCTCAC 
CTGGTGGCTC CTGGTGAAGA AGGGGCTAGT GGGGCAGGAC TGGGTCCCAG CAAGCGCGTC 
TTGGGATCTC TGTTGTCTGA CTCCCTGTTT CTTGAAGTAA AAGAACTCTT CCTGGCAGCT 
GTGCGTCTGG ACCCTACATC CATTGACCCT GACGTGCAGT GTGGCCTGGG AGTCCTCTTC 
AACCTGAGTG GGGAGTACGA CAAGGCTGTG GACTGTTTCA CGGCTGCCCT CAGTGTCCGC 
CCCAATGACT ATTTGCTGTG GAACAAGCTT GGGGCCACCC TGGCCAACGG GAACCAGAGT 
GAAGAAGCAG TGGCTGCTTA CCGCAGAGCC CTTGAACTAC AGCCTGGGTA CATCCGCTCC 
CGCTACAACC TAGGCATCAG CTGCATCAAC CTTGGGGCTC ACCGTGAGGC CGTGGAACAC 
TTTCTGGAGG CCCTGAACAT GCAGAGGAAG AGCCGGGGCC CTCGGGGTGA GGGGGGCGCC 
ATGTCTGAGA ACATCTGGAG TACTCTGCGG TTGGCATTGT CCATGCTAGG CCAGAGTGAT 
GCCTATGGGG CAGCCGATGC TCGGGACCTG CCTGCCCTTC TAACTATGTT TGGCCTGCCC 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pex5 ( XM_006237384.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006237446.1 Rattus norvegicus peroxisomal biogenesis factor 5 (Pex5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa27779
Clone ID Related Accession (Same CDS sequence)	XM_006237385.3 , XM_006237384.2
Accession Version	XM_006237384.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1926bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	peroxisomal targeting signal 1 receptor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006237384.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAATGC GGGAGCTGGT GGAGAGCGAA TGTGGGGGTG CCAACCCGCT GATGAAGCTG 
GCCACCCACT TCACCCAGGA CAAGGCCCTT CGGCAGGAGG GACTGAGGCC TGGCCCCTGG 
CCTCCAGGAG CCTCAGCGGC GGAAACGGTT TCCAAGCCTT TGGGAGTAGG CACTGAAGAT 
GAGTTGGTGG CAGAATTCCT GCAGGACCAG AATGCAACTC TTGTATCCCG AGCCCCTCAG 
ACCTTCAAGA TGGATGACCT CTTGGCGGAG ATGCAGGAGA TTGAACAGTC AAACTTTCGC 
CAGGCTCCTC AGAGAGCCCC TGGTGTGGCA GACTTGGCTT TGTCTGAGAA TTGGGCCCAG 
GAGTTTCTTG CCGCTGGAGA TGCTGTGGAT GTAGCTCAGG ATTATAACGA GACCGACTGG 
TCCCAAGAAT TCATCGCCGA AGTCACGGAC CCTCTGTCTG TGTCCCCAGC CCGATGGGCT 
GAGGAGTATC TGGAACAGTC TGAGGAGAAG CTGTGGTTGG GAGATCCAGA AGGAACCTCT 
ACCACTGACC GATGGTACGA TGAATACCAT CCTGAGGAGG ATCTGCAGCA CACAGCCAGT 
GACTTTGTGT CCAAGGTGGA CGACCCCAAA TTGGCTAACT CTGAGTTCCT GAAATTCGTG 
CGGCAGATTG GCGAGGGGCA GGTGTCCCTG GAGTCCGCTG CAGGGTCGGG CCGAGCTCAG 
GCAGAACAGT GGGCAGCAGA GTTTATACAG CAGCAGGGCA CATCGGAGGC CTGGGTTGAT 
CAGTTCACAA GACCAGGAAA CAAACTGGCT GCTCTTCAGG TGGAATTTGA ACGAGCCAAG 
TCAGCTATAG AGTCAGACGT CGATTTCTGG GACAAGTTGC AGGCAGAGTT GGAGGAGATG 
GCAAAACGGG ATGCTGAGGC GCACCCCTGG CTTTCTGACT ATGACGACCT CACGTCTGCT 
TCCTATGACA AGGGCTACCA GTTTGAGGAG GAGAATCCCT TGCGTGACCA CCCTCAGCCT 
TTTGAAGAAG GGCTGCGTCG ACTTGAGGAG GGAGACCTGC CCAATGCTGT GCTGCTCTTC 
GAGGCTGCGG TGCAGCAGGA TCCTAAGCAC ATGGAGGCCT GGCAGTACCT GGGCACCACT 
CAGGCTGAGA ACGAGCAAGA GCTCTTGGCC ATCAGTGCCC TGCGCAGGTG TCTGGAGCTA 
AAGCCAGATA ACCGGACAGC ACTGATGGCG CTGGCTGTAA GCTTCACCAA TGAGTCCCTG 
CAGCGGCAAG CCTGTGAGAC GCTGCGGGAC TGGCTGCGCT ACTCACCGGC CTACGCTCAC 
CTGGTGGCTC CTGGTGAAGA AGGGGCTAGT GGGGCAGGAC TGGGTCCCAG CAAGCGCGTC 
TTGGGATCTC TGTTGTCTGA CTCCCTGTTT CTTGAAGTAA AAGAACTCTT CCTGGCAGCT 
GTGCGTCTGG ACCCTACATC CATTGACCCT GACGTGCAGT GTGGCCTGGG AGTCCTCTTC 
AACCTGAGTG GGGAGTACGA CAAGGCTGTG GACTGTTTCA CGGCTGCCCT CAGTGTCCGC 
CCCAATGACT ATTTGCTGTG GAACAAGCTT GGGGCCACCC TGGCCAACGG GAACCAGAGT 
GAAGAAGCAG TGGCTGCTTA CCGCAGAGCC CTTGAACTAC AGCCTGGGTA CATCCGCTCC 
CGCTACAACC TAGGCATCAG CTGCATCAAC CTTGGGGCTC ACCGTGAGGC CGTGGAACAC 
TTTCTGGAGG CCCTGAACAT GCAGAGGAAG AGCCGGGGCC CTCGGGGTGA GGGGGGCGCC 
ATGTCTGAGA ACATCTGGAG TACTCTGCGG TTGGCATTGT CCATGCTAGG CCAGAGTGAT 
GCCTATGGGG CAGCCGATGC TCGGGACCTG CCTGCCCTTC TAACTATGTT TGGCCTGCCC 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006237446.1
CDS	208..2133
Translation	MAMRELVESECGGANPLMKLATHFTQDKALRQEGLRPGPWPPGASAAETVSKPLGVGTEDELVAEFLQDQNATLVSRAPQTFKMDDLLAEMQEIEQSNFRQAPQRAPGVADLALSENWAQEFLAAGDAVDVAQDYNETDWSQEFIAEVTDPLSVSPARWAEEYLEQSEEKLWLGDPEGTSTTDRWYDEYHPEEDLQHTASDFVSKVDDPKLANSEFLKFVRQIGEGQVSLESAAGSGRAQAEQWAAEFIQQQGTSEAWVDQFTRPGNKLAALQVEFERAKSAIESDVDFWDKLQAELEEMAKRDAEAHPWLSDYDDLTSASYDKGYQFEEENPLRDHPQPFEEGLRRLEEGDLPNAVLLFEAAVQQDPKHMEAWQYLGTTQAENEQELLAISALRRCLELKPDNRTALMALAVSFTNESLQRQACETLRDWLRYSPAYAHLVAPGEEGASGAGLGPSKRVLGSLLSDSLFLEVKELFLAAVRLDPTSIDPDVQCGLGVLFNLSGEYDKAVDCFTAALSVRPNDYLLWNKLGATLANGNQSEEAVAAYRRALELQPGYIRSRYNLGISCINLGAHREAVEHFLEALNMQRKSRGPRGEGGAMSENIWSTLRLALSMLGQSDAYGAADARDLPALLTMFGLPQ

Target ORF information:

RefSeq Version	XM_006237384.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus peroxisomal biogenesis factor 5 (Pex5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006237384.2

ORF Insert Sequence:

ATGGCAATGC GGGAGCTGGT GGAGAGCGAA TGTGGGGGTG CCAACCCGCT GATGAAGCTG 
GCCACCCACT TCACCCAGGA CAAGGCCCTT CGGCAGGAGG GACTGAGGCC TGGCCCCTGG 
CCTCCAGGAG CCTCAGCGGC GGAAACGGTT TCCAAGCCTT TGGGAGTAGG CACTGAAGAT 
GAGTTGGTGG CAGAATTCCT GCAGGACCAG AATGCAACTC TTGTATCCCG AGCCCCTCAG 
ACCTTCAAGA TGGATGACCT CTTGGCGGAG ATGCAGGAGA TTGAACAGTC AAACTTTCGC 
CAGGCTCCTC AGAGAGCCCC TGGTGTGGCA GACTTGGCTT TGTCTGAGAA TTGGGCCCAG 
GAGTTTCTTG CCGCTGGAGA TGCTGTGGAT GTAGCTCAGG ATTATAACGA GACCGACTGG 
TCCCAAGAAT TCATCGCCGA AGTCACGGAC CCTCTGTCTG TGTCCCCAGC CCGATGGGCT 
GAGGAGTATC TGGAACAGTC TGAGGAGAAG CTGTGGTTGG GAGATCCAGA AGGAACCTCT 
ACCACTGACC GATGGTACGA TGAATACCAT CCTGAGGAGG ATCTGCAGCA CACAGCCAGT 
GACTTTGTGT CCAAGGTGGA CGACCCCAAA TTGGCTAACT CTGAGTTCCT GAAATTCGTG 
CGGCAGATTG GCGAGGGGCA GGTGTCCCTG GAGTCCGCTG CAGGGTCGGG CCGAGCTCAG 
GCAGAACAGT GGGCAGCAGA GTTTATACAG CAGCAGGGCA CATCGGAGGC CTGGGTTGAT 
CAGTTCACAA GACCAGGAAA CAAACTGGCT GCTCTTCAGG TGGAATTTGA ACGAGCCAAG 
TCAGCTATAG AGTCAGACGT CGATTTCTGG GACAAGTTGC AGGCAGAGTT GGAGGAGATG 
GCAAAACGGG ATGCTGAGGC GCACCCCTGG CTTTCTGACT ATGACGACCT CACGTCTGCT 
TCCTATGACA AGGGCTACCA GTTTGAGGAG GAGAATCCCT TGCGTGACCA CCCTCAGCCT 
TTTGAAGAAG GGCTGCGTCG ACTTGAGGAG GGAGACCTGC CCAATGCTGT GCTGCTCTTC 
GAGGCTGCGG TGCAGCAGGA TCCTAAGCAC ATGGAGGCCT GGCAGTACCT GGGCACCACT 
CAGGCTGAGA ACGAGCAAGA GCTCTTGGCC ATCAGTGCCC TGCGCAGGTG TCTGGAGCTA 
AAGCCAGATA ACCGGACAGC ACTGATGGCG CTGGCTGTAA GCTTCACCAA TGAGTCCCTG 
CAGCGGCAAG CCTGTGAGAC GCTGCGGGAC TGGCTGCGCT ACTCACCGGC CTACGCTCAC 
CTGGTGGCTC CTGGTGAAGA AGGGGCTAGT GGGGCAGGAC TGGGTCCCAG CAAGCGCGTC 
TTGGGATCTC TGTTGTCTGA CTCCCTGTTT CTTGAAGTAA AAGAACTCTT CCTGGCAGCT 
GTGCGTCTGG ACCCTACATC CATTGACCCT GACGTGCAGT GTGGCCTGGG AGTCCTCTTC 
AACCTGAGTG GGGAGTACGA CAAGGCTGTG GACTGTTTCA CGGCTGCCCT CAGTGTCCGC 
CCCAATGACT ATTTGCTGTG GAACAAGCTT GGGGCCACCC TGGCCAACGG GAACCAGAGT 
GAAGAAGCAG TGGCTGCTTA CCGCAGAGCC CTTGAACTAC AGCCTGGGTA CATCCGCTCC 
CGCTACAACC TAGGCATCAG CTGCATCAAC CTTGGGGCTC ACCGTGAGGC CGTGGAACAC 
TTTCTGGAGG CCCTGAACAT GCAGAGGAAG AGCCGGGGCC CTCGGGGTGA GGGGGGCGCC 
ATGTCTGAGA ACATCTGGAG TACTCTGCGG TTGGCATTGT CCATGCTAGG CCAGAGTGAT 
GCCTATGGGG CAGCCGATGC TCGGGACCTG CCTGCCCTTC TAACTATGTT TGGCCTGCCC 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Identification of ubiquitin-specific protease 9X (USP9X) as a deubiquitinase acting on ubiquitin-peroxin 5 (PEX5) thioester conjugate.
The Journal of biological chemistry287(16)12815-27(2012 Apr)
Grou CP,Francisco T,Rodrigues TA,Freitas MO,Pinto MP,Carvalho AF,Domingues P,Wood SA,Rodríguez-Borges JE,Sá-Miranda C,Fransen M,Azevedo JE

PEX5 protein binds monomeric catalase blocking its tetramerization and releases it upon binding the N-terminal domain of PEX14.
The Journal of biological chemistry286(47)40509-19(2011 Nov)
Freitas MO,Francisco T,Rodrigues TA,Alencastre IS,Pinto MP,Grou CP,Carvalho AF,Fransen M,Sá-Miranda C,Azevedo JE

PEX14 is required for microtubule-based peroxisome motility in human cells.
Journal of cell science124(Pt 10)1759-68(2011 May)
Bharti P,Schliebs W,Schievelbusch T,Neuhaus A,David C,Kock K,Herrmann C,Meyer HE,Wiese S,Warscheid B,Theiss C,Erdmann R

The Peroxisomal Targeting Signal 1 in sterol carrier protein 2 is autonomous and essential for receptor recognition.
BMC biochemistry1212(2011 Mar)
Williams CP,Schueller N,Thompson CA,van den Berg M,Van Haren SD,Erdmann R,Bond CS,Distel B,Schliebs W,Wilmanns M,Stanley WA

A proteome-wide perspective on peroxisome targeting signal 1(PTS1)-Pex5p affinities.
Journal of the American Chemical Society132(11)3973-9(2010 Mar)
Ghosh D,Berg JM

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Pex5 cDNA ORF clone, Rattus norvegicus(Norway rat)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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