Knl1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Knl1
Entrez Gene ID	311327
Full Name	kinetochore scaffold 1
Synonyms	Casc5
General protein information	Preferred Names kinetochore scaffold 1 Names protein CASC5 RAD51 homolog cancer susceptibility candidate 5
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q35

mRNA and Protein(s)

mRNA	Protein	Name
XM_008762098.1	XP_008760320.1	protein CASC5 isoform X3
XM_008762099.2	XP_008760321.1	protein CASC5 isoform X2
NM_001170594.1	NP_001164065.1	protein CASC5
XM_008762095.1	XP_008760317.1	protein CASC5 isoform X1
XM_008762097.2	XP_008760319.1	protein CASC5 isoform X2
XM_008762096.1	XP_008760318.1	protein CASC5 isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	CASC5	NP_733468.3
Pan troglodytes (chimpanzee)	CASC5	XP_510312.3
Canis lupus familiaris (dog)	CASC5	XP_005638312.1
Bos taurus (cattle)	CASC5	XP_002690867.3
Mus musculus (house mouse)	Casc5	NP_083893.2
Rattus norvegicus (Norway rat)	Casc5	NP_001164065.1
Gallus gallus (chicken)	CASC5	XP_420938.4

Gene Ontology
Bibliography

Related articles in PubMed

Kinetochore KMN network gene CASC5 mutated in primary microcephaly.
Genin A, Desir J, Lambert N, Biervliet M, Van Der Aa N, Pierquin G, Killian A, Tosi M, Urbina M, Lefort A, Libert F, Pirson I, Abramowicz M
Human molecular genetics21(24)5306-17(2012 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Knl1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Knl1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa06355	NM_001170594.1 Latest version!	Rattus norvegicus kinetochore scaffold 1 (Knl1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ORa26224	XM_008762095.1 Latest version!	Rattus norvegicus kinetochore scaffold 1 (Knl1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ORa46967	XM_008762099.2 Latest version!	Rattus norvegicus kinetochore scaffold 1 (Knl1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ORa46967	XM_008762096.1 Latest version!	Rattus norvegicus kinetochore scaffold 1 (Knl1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ORa46967	XM_008762097.2 Latest version!	Rattus norvegicus kinetochore scaffold 1 (Knl1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ORa46968	XM_008762098.1 Latest version!	Rattus norvegicus kinetochore scaffold 1 (Knl1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Knl1 ( NM_001170594.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001164065.1 Rattus norvegicus kinetochore scaffold 1 (Knl1), mRNA.

Price & Availability↑

CloneID	ORa06355
Clone ID Related Accession (Same CDS sequence)	NM_001170594.1
Accession Version	NM_001170594.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6345bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1477670400000
Organism	Rattus norvegicus(Norway rat)
Product	protein CASC5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC111293.6. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence. The extent of this transcript is supported by orthologous data. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052297, SAMEA2689594 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATGGGA TATATTCAGA GGCTAATGAA GAGAATGACA ATACACAGAG GCCTGTTAGA 
AGACAGCATT CCTCAATTTT GAAACCCCCA AGGAGTCCTC TTCAGGACCT CAAATGTGGA 
AATGAAATAG TTCAGGAATC TAATGCTCCG AGAACCAGGA AAAGCTCTCG TCGGGTCAGC 
TTTGCAGATA CTATAAAAGT ATTCCAGACA GAGTCCCATA TGAAAACGGA AAAAAATTCA 
GAAATTTCAG GAATGAACAC TTTGCTTTGT GCTCCAATTC AGACTCAGAT GCAACAAAAG 
GAGTTTTCAA TTACAGACTG CAACCCTGAA AGGAAACATG CAAATGATCA AACAGTCATC 
TTTTCAGATG AAAATCAGAT GGATCTGACA GCAAGTCACA CGGTGATGAT TGCCAAAGGT 
CTTGTAGACT GTACCAAACA TGAAAATTCC ACAAAAATAG ATACCACATC CTTTCTGGAG 
AATTTAAAGC ATCGTGCTGC GAATTCAAGA GTTAAAAACG ATTTAGCATG TCCAATAATT 
TCTATAAATC AAAACATTTT TTCAGAAAAG AAAAATTCTG ACAACTTCAT AAAAAGATTG 
AAAATGGGGA AATATAATAC TTCTTCTACA GAACTTGATA AGGAAAATGC TGAAATACCT 
GTTCATTCCA AAGACTCAAA CAGTGCCTCT TCCACGTGTC AAATACATGC ATCGCTTAGT 
GTCGATGAAC ATAGTAGTAA TAAGACTAGA CTCTTTACAG AACAAGATGA TGGGATGAAT 
TTGACCCAGT GTCATACAGC CTGTATTCAG ACTTGGATTC CTCCATCCAG GGAGGCCACC 
TTGGGAGAGG TTAAAGGTGA TAATACAATT TATGGCAATG AGTGTATGGA GTTGACAACT 
AACTACACGA TACAGGTGTT GTCATCAGAA AATAACTTAC CTGAAAGAGA AAATCAAGCT 
CAAAATGATG TGATTGATGT TACAACAGTC GATGGAGCTA CAGCTCCAGA GAAAAAAACA 
GCCCTTAAGG ATAAACTAAA TGCTGCTTTT CACAGCTCTT TCCCAAACCC TGAAAATAAA 
CTTCATATTA CCAAATGTCA TCCTATAGAG TCAGAAACTC ATACAGTCAC ACAGATTTCT 
AATCAAAATG CCAACACATT GGCTATGACC TCAGAATCTG TATGTTCTAG CCCAGCTATT 
CAAGGGTATA AAACAATTTT TTACTCTAGT AGCAATGATG CAATGGAACT GACTAAGTGT 
CTATCAATTA TGCAAGAGGA AAAAAAATTG CTGAAGACTG ATGATAAGTA TTCTAAAATA 
TGTACCAATT CAGATGCTAG CCCACTCAGA GAGAAAACTA TTTATTCAGA AGAGGATAGC 
ATGGACATTA CCAAAAGTCA CACAGTTGCA ATAGATAATA AGATATTTAA ACATGATCAA 
GAAAACATTA AAAAAGAAAT AACAGCTCTA CCAATATTTG AAAAAGAAAT GATGCTCCAA 
AATCTTAAAA CTATGTCCAA AGATGAGAAA ATGAATGTGA ATTCTATTAC AGTTCCTCAA 
GTATCTAAAG AAAGTTTACA ACAGAGTCTG ACAAATGCCT TATCTATTTC ATTGGCTGAT 
AAAACAATGG AATTCTTAGC AGGTGAAGAT ATGGATTTGA CTAAAAGTCA CACAACTAAG 
TTAAGTCAAG TTATTCCTAC AACTTTTGAC TTAGCATCAA AGAATGTCAC CAAATCTTCC 
TCTCACAGCA AAAGCCCTTC AGATGAGTGG GAAGGCCTGG AAAAACAAAT TGTGCTAGGC 
CAGCATTCTA AACTTCCTTC ACCACAGAGG AAAGATTTAG ATGATCCTGA CTGTTCTCAT 
CATAAGATAA TGTATTCAGA GGAGGTACAA ACTATGGATC TAACCAAGAG CCACACTATT 
GTGATTGGGT TTGGTTCTTC TGAGGTACAA GAACACAGTA AGATTAATTT AGAACACAAA 
AGCAGCCAAC TTACAGCAGA AAACAGACAA ACAGCTGTGA ACATTCCTGG AATAAACTTT 
AGAGTAGTAA CAACTAATGA TATGGACATG TTAAAGGACA GAAGTATACA TAAGCCAGAA 
TTGCTGGAGG AAAAGCAAAA CGTCAAAATT TATGGAAGAA AAAGTATGGG TGGACTGAAG 
ATTGATAAAA CTATATTATT TTCAGAGGGT AATGAGGATG ATATGGATAT CACTAAGAGT 
TGCACAGTCA AAATAAACCA TCGGTCTTTA TTAGATAAAC ATGATTCTCA TTTAGTATCT 
TTGGCAGGAA CTTCTAAAAC CATTTTGCAT GCACGTGGAC AGGATGAAAT GGAAATTAAT 
AGAAATCACA CGACTCCTTT AGAGTGTAAA ATTATCTCAC CCAGTGATAT AACTTCTGGT 
GATCTGAGTA AGACTGTGAT GTCTATAGAT GATCATGAGG AACTAGAAAT GACTAAGTCC 
CATACTGTTT TCATTGACTA CCAAGCAGAG GCAAAAGGTG TACTTCCAGA CAGACTTGAC 
TTTCAATTAT CCAAAAAGAA AAGCCTACAG AAGCCAAAAA TGACATCTAC TCATGCTGAA 
GAGATTGTTT CCATTTCAAA AAATAGTGAA AGCAACTATC TACCAGCCAA AGGCAGCCAG 
GTAACAATAT TAGAGGAAGG ATCTAATAGT GGGCCTGGGG AAGAAACTAA TGATGCACAA 
AAGCCTGGAT TTCTGAATGA ACCTCTGTCA GGCAAAACTC AAAGAAGAGA AAGCCTTAAG 
CTCAAAAATA AGACTATTAC ATTTTCAGGA AATGATAAAA GTAATAGAGA TATCACTCAG 
AGTTCTGTGG TAGAAATAAA TAATGAAACT AACCTGGAAG ATAGAAAGGG CTTCAGTTTT 
GTGCCATTGG CAGGAACTTC CAAGCCAGTT TTATCTGCAT ATCGGCCAGA GGACATGGAG 
ATCTCTATAA GTCAAACCTC TGCTTCGGAG TATAAAACTA TCCCACCAGA TGAAATGACT 
ACTATACCTA TGGATAAAAC TGTAATGTTT GTAGATAATC TCAGTGATCT GGGTGTCACC 
AGATCCCATA CGTTTTTCAT CGATTGTCAA GCCAAAGAGA AAGTACTTCA TGAATATACC 
AACTTGGGAA TACCAAAACC ACAAAACTTG AGTGGTTCTG AAGATAATTC CTGTATTCAA 
GAAATCACCA AAAAACCAGT AGCTGAATAC AAACACCATA TGACAACTGT TATTCCTTCT 
AATACATTAT TTAGTGATCA AAGTACAATG AAAATCAAGA TGCATAAAGC TGATATAGAT 
GAAGAGGGCA TAGGAAAGGA AGTGGAGGCC GATACATTGA AACAAACTAA ACTTGAAAGG 
TGTCTGTTAA ATAGAACTGA TGGAAAAAAT GTGGATTTTA CAAGTAGTCA TATAGCTGAT 
GTTTGTACAT CCAGAGACAA GTATTCCTCT TTACCAAATG TTATTTCTTC TTCTGATAAT 
TTAGATAGTA ATACTAAGTC CTTGTGTGAT AAAAGTAGGG AGAAAGCTTA TAATTGCCAA 
GTGCCAAATG AGTTTACTTT TGCAGATAAT TTACCAAGTA GATATCACCT GGATTCTGAA 
ATACCATCTG TCTTTGTATC ATGTCCTTCA AAAGAAGTTA CTCAAACTGA AAGTGCCATA 
GCACTACTTC AAGATCAAGA TCAAGAGCCT CTGGGACTCT CAAAGTGTGC ATCTCTTAAG 
TGTGCAAAGG ATCAAATGGA GGCCTTTGTT GATGTTAGTG TTGCTTCTCA GCCTCATCTC 
TCAGCCCAAC AACCTCCATC AACTCAAAAA GGGCAGGATG CCGCCAATAG AGATGAAGGA 
ATGCTATCTA AAGCTGGGAA GAAGACTTTA CCTTTTCTTA TAGGAAATGT CTCTGCACCC 
ACATGTGAAA ATGCGTCCAA AATGCCTACT AATGAGGAAC ACTTTGCTGT AACCTATAAC 
AAAGAACTGG AAAAAAGTAT TCAAACAGAT AAATGTAATA CAAATGTACA CAACCATAGT 
AACTCAGTCC TGACAACACA AGTCATTCAA ACACATGCCA GTGCCGAAGC AGCATCAGAC 
TCTATAGTTC CATCTAATGG TTCCTGTTTT AGTAGTGCCA AACCAAGTCT GAGTAATTTG 
AATAGAAAAA CTGGGAAGGT TTTAGACTTT AATACTGTTC ATATATTACC TCCTGCAGAA 
CAGTTACTTG AAGCAGGAAA TCAGGCACAT AGTATGAGTA TAGTACAAGG TACAGAAATA 
CATAGCCTAG GTTCCAGCAA TGACAGAGAT AAAGAAAGTA AAACTCTGCA TAATGAGGCC 
GAAGCTACAT CTGTACCACT AAAAGCAGTT GTTAAAGACA AAGCAAGAAG ATGTTCATTG 
GGAATATTTT TACCCAAATT ACCAAACAAG AAAAGTTGTA GTATCACTGG TGTTGATGAT 
GTGGAACAGA TTCTAGCAGA TGCAACTGAC TTAACTCACC TAGAAACTCA GCCAGTCTGT 
AGTCAAGATC CGGGCATTGG CTCTGTTGCA GCCAAACTGA ACTTGAGTCC ATCTCAGTAT 
ATAAATGAGG AGAATCTCCC TGTATATCCC GGTGAGATAC TTTCATCAGA TTCTGTCATC 
CTGGACATTG AGGAAAGTGC TCCGATCGAC ACATATCATA AAGAGATTTT ACCATCTGAA 
AACAAAACAG AAACCTGCAG GGCCCAAAAA AGAGCCTGGG TTCAAGAAAA TGAGGTTAAA 
AATGAGAAAA AAATCAGAAC ACATGATGGT GCACAAGATC AAGAGATTTT TGATCATTAT 
ACTGAAGAGG ACATCAATAA AAGTATTAAC AATGTATTTC TAAAAAGCCT GAGCAGGACA 
CCGTCTAGTT CTAGCAGCTC TCTGGATTCA ATCAAGGCTG ATGGAATCTC TCTGGATGCC 
AGCACACAGC GTAATAGCCA AATGGAATCA CAGTTTCTTG GTGACACTAT CTGTGAAGAG 
AGTTTGAGGG AGAAACTGAA GGATGGACAA ATTACAATCA AAGAGTTCTT CATACTTCTT 
CAAGTTCATA TCTTAATACA GAAACCTAGG CAGAGCACTC TCCCAGCCAA ATTTACTATA 
AACACATTAC CTACCACAGA AGATCTGATG TTGAGACAAT ATGTTTACGG ACCCAAGATA 
CAAATTTACA GAGAAGATTG TGAGCTTCTT CGACAGAAGA TTGAAGAATT GAAGGTATCT 
GCGTTGAACC AAGATAAACT ATTGGTGGAT GTAAATAGGA ACTTGTGGGA AAAAATGAAA 
GACTACTCTG ACGAAGAGCT GAAGACCTAT GGAATTTACC TTAACAAAAT AAAATCACGT 
TATACCAAGA TGACGAAAGT CTTCACTCAC CAAGGAAAAG TGGATCTGTA TAGCAAATTG 
GTGCACTCAG CTGAGACTGA GAAGAGGAAA CTTCAGATAA AAATCAACGA GATGGACACC 
ATACTTAAGA AGATCAATAA CTGTCTCACT GCAGTGGAAA TAGAAACTAA GAATTTGGAG 
GCTGAAGAGA AAGATGATGC CATGGAAGAT TGGGATTCTG AAATACGAGA TGCAGAGAAA 
GAACTGGAAA GGCTGAAAAC TGAGGAAGAG GAGCTTCAAA GAAAGTTCTT AGAAGTAGAG 
ACACAGAAAA CGCAGACCCT TGCTCAGATA GAATTTATAA AAGAACAAAC AACTGAAACA 
GAAGAATTAT TGGATCAATT GAGTTTTTCT GAGTGGGATA TCATCGAGTG GAGTGATGAA 
CAAGCGGTAT TTACCTTTGT TTATGATTCA ATAGAACTTA TCATCACTTT TGGAGAGCCT 
CAGGTTGGTC TCCCTTTCTT GGACAAGGCT TACAGGACGA TTAATGAACT CAGTTTTCAG 
TCTCTGTTAG ACGAAGATAA AGCGCCTCCT TCCTCCCTCT TGGTCCACAA GCTTATTTTC 
CAGTACATTG AAGAGCAGGA GTTCTGGAAG AAGAAGTGCA CAACACAGCA TCAGGTGCCC 
CAGATGCTTC AAGAACTCTC ATTGGTAGTT AACCATTGTA GGCTTCTTGG AGAAGAGATT 
GAATTTTTAA AGCGATGGGG ACCAAATTAT AGCCTAATGC ATATAAATGT GAATAATACT 
GAATTACGGC TTCTATTCTC CAGTTCTGCA GCATTTGCAA AGTTTGAGAT AACTTTGTTT 
CCCTCGGCCC ATTATCCCTT GGTCCCACTA CCATTCACCA TTCAAAATCA CCTTGGGAAG 
ACTGGCCATG ATGAAATTGC TGCCGTTATC TCTAAAGTGC CCTTAGAGGA AAACTACCTG 
AAGAATGTAG TCAAGCAGAT TTACCAAGAT CTACTTAAGG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001164065.1
CDS	1..6345
Translation	MDGIYSEANEENDNTQRPVRRQHSSILKPPRSPLQDLKCGNEIVQESNAPRTRKSSRRVSFADTIKVFQTESHMKTEKNSEISGMNTLLCAPIQTQMQQKEFSITDCNPERKHANDQTVIFSDENQMDLTASHTVMIAKGLVDCTKHENSTKIDTTSFLENLKHRAANSRVKNDLACPIISINQNIFSEKKNSDNFIKRLKMGKYNTSSTELDKENAEIPVHSKDSNSASSTCQIHASLSVDEHSSNKTRLFTEQDDGMNLTQCHTACIQTWIPPSREATLGEVKGDNTIYGNECMELTTNYTIQVLSSENNLPERENQAQNDVIDVTTVDGATAPEKKTALKDKLNAAFHSSFPNPENKLHITKCHPIESETHTVTQISNQNANTLAMTSESVCSSPAIQGYKTIFYSSSNDAMELTKCLSIMQEEKKLLKTDDKYSKICTNSDASPLREKTIYSEEDSMDITKSHTVAIDNKIFKHDQENIKKEITALPIFEKEMMLQNLKTMSKDEKMNVNSITVPQVSKESLQQSLTNALSISLADKTMEFLAGEDMDLTKSHTTKLSQVIPTTFDLASKNVTKSSSHSKSPSDEWEGLEKQIVLGQHSKLPSPQRKDLDDPDCSHHKIMYSEEVQTMDLTKSHTIVIGFGSSEVQEHSKINLEHKSSQLTAENRQTAVNIPGINFRVVTTNDMDMLKDRSIHKPELLEEKQNVKIYGRKSMGGLKIDKTILFSEGNEDDMDITKSCTVKINHRSLLDKHDSHLVSLAGTSKTILHARGQDEMEINRNHTTPLECKIISPSDITSGDLSKTVMSIDDHEELEMTKSHTVFIDYQAEAKGVLPDRLDFQLSKKKSLQKPKMTSTHAEEIVSISKNSESNYLPAKGSQVTILEEGSNSGPGEETNDAQKPGFLNEPLSGKTQRRESLKLKNKTITFSGNDKSNRDITQSSVVEINNETNLEDRKGFSFVPLAGTSKPVLSAYRPEDMEISISQTSASEYKTIPPDEMTTIPMDKTVMFVDNLSDLGVTRSHTFFIDCQAKEKVLHEYTNLGIPKPQNLSGSEDNSCIQEITKKPVAEYKHHMTTVIPSNTLFSDQSTMKIKMHKADIDEEGIGKEVEADTLKQTKLERCLLNRTDGKNVDFTSSHIADVCTSRDKYSSLPNVISSSDNLDSNTKSLCDKSREKAYNCQVPNEFTFADNLPSRYHLDSEIPSVFVSCPSKEVTQTESAIALLQDQDQEPLGLSKCASLKCAKDQMEAFVDVSVASQPHLSAQQPPSTQKGQDAANRDEGMLSKAGKKTLPFLIGNVSAPTCENASKMPTNEEHFAVTYNKELEKSIQTDKCNTNVHNHSNSVLTTQVIQTHASAEAASDSIVPSNGSCFSSAKPSLSNLNRKTGKVLDFNTVHILPPAEQLLEAGNQAHSMSIVQGTEIHSLGSSNDRDKESKTLHNEAEATSVPLKAVVKDKARRCSLGIFLPKLPNKKSCSITGVDDVEQILADATDLTHLETQPVCSQDPGIGSVAAKLNLSPSQYINEENLPVYPGEILSSDSVILDIEESAPIDTYHKEILPSENKTETCRAQKRAWVQENEVKNEKKIRTHDGAQDQEIFDHYTEEDINKSINNVFLKSLSRTPSSSSSSLDSIKADGISLDASTQRNSQMESQFLGDTICEESLREKLKDGQITIKEFFILLQVHILIQKPRQSTLPAKFTINTLPTTEDLMLRQYVYGPKIQIYREDCELLRQKIEELKVSALNQDKLLVDVNRNLWEKMKDYSDEELKTYGIYLNKIKSRYTKMTKVFTHQGKVDLYSKLVHSAETEKRKLQIKINEMDTILKKINNCLTAVEIETKNLEAEEKDDAMEDWDSEIRDAEKELERLKTEEEELQRKFLEVETQKTQTLAQIEFIKEQTTETEELLDQLSFSEWDIIEWSDEQAVFTFVYDSIELIITFGEPQVGLPFLDKAYRTINELSFQSLLDEDKAPPSSLLVHKLIFQYIEEQEFWKKKCTTQHQVPQMLQELSLVVNHCRLLGEEIEFLKRWGPNYSLMHINVNNTELRLLFSSSAAFAKFEITLFPSAHYPLVPLPFTIQNHLGKTGHDEIAAVISKVPLEENYLKNVVKQIYQDLLKD

Target ORF information:

RefSeq Version	NM_001170594.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus kinetochore scaffold 1 (Knl1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170594.1

ORF Insert Sequence:

ATGGATGGGA TATATTCAGA GGCTAATGAA GAGAATGACA ATACACAGAG GCCTGTTAGA 
AGACAGCATT CCTCAATTTT GAAACCCCCA AGGAGTCCTC TTCAGGACCT CAAATGTGGA 
AATGAAATAG TTCAGGAATC TAATGCTCCG AGAACCAGGA AAAGCTCTCG TCGGGTCAGC 
TTTGCAGATA CTATAAAAGT ATTCCAGACA GAGTCCCATA TGAAAACGGA AAAAAATTCA 
GAAATTTCAG GAATGAACAC TTTGCTTTGT GCTCCAATTC AGACTCAGAT GCAACAAAAG 
GAGTTTTCAA TTACAGACTG CAACCCTGAA AGGAAACATG CAAATGATCA AACAGTCATC 
TTTTCAGATG AAAATCAGAT GGATCTGACA GCAAGTCACA CGGTGATGAT TGCCAAAGGT 
CTTGTAGACT GTACCAAACA TGAAAATTCC ACAAAAATAG ATACCACATC CTTTCTGGAG 
AATTTAAAGC ATCGTGCTGC GAATTCAAGA GTTAAAAACG ATTTAGCATG TCCAATAATT 
TCTATAAATC AAAACATTTT TTCAGAAAAG AAAAATTCTG ACAACTTCAT AAAAAGATTG 
AAAATGGGGA AATATAATAC TTCTTCTACA GAACTTGATA AGGAAAATGC TGAAATACCT 
GTTCATTCCA AAGACTCAAA CAGTGCCTCT TCCACGTGTC AAATACATGC ATCGCTTAGT 
GTCGATGAAC ATAGTAGTAA TAAGACTAGA CTCTTTACAG AACAAGATGA TGGGATGAAT 
TTGACCCAGT GTCATACAGC CTGTATTCAG ACTTGGATTC CTCCATCCAG GGAGGCCACC 
TTGGGAGAGG TTAAAGGTGA TAATACAATT TATGGCAATG AGTGTATGGA GTTGACAACT 
AACTACACGA TACAGGTGTT GTCATCAGAA AATAACTTAC CTGAAAGAGA AAATCAAGCT 
CAAAATGATG TGATTGATGT TACAACAGTC GATGGAGCTA CAGCTCCAGA GAAAAAAACA 
GCCCTTAAGG ATAAACTAAA TGCTGCTTTT CACAGCTCTT TCCCAAACCC TGAAAATAAA 
CTTCATATTA CCAAATGTCA TCCTATAGAG TCAGAAACTC ATACAGTCAC ACAGATTTCT 
AATCAAAATG CCAACACATT GGCTATGACC TCAGAATCTG TATGTTCTAG CCCAGCTATT 
CAAGGGTATA AAACAATTTT TTACTCTAGT AGCAATGATG CAATGGAACT GACTAAGTGT 
CTATCAATTA TGCAAGAGGA AAAAAAATTG CTGAAGACTG ATGATAAGTA TTCTAAAATA 
TGTACCAATT CAGATGCTAG CCCACTCAGA GAGAAAACTA TTTATTCAGA AGAGGATAGC 
ATGGACATTA CCAAAAGTCA CACAGTTGCA ATAGATAATA AGATATTTAA ACATGATCAA 
GAAAACATTA AAAAAGAAAT AACAGCTCTA CCAATATTTG AAAAAGAAAT GATGCTCCAA 
AATCTTAAAA CTATGTCCAA AGATGAGAAA ATGAATGTGA ATTCTATTAC AGTTCCTCAA 
GTATCTAAAG AAAGTTTACA ACAGAGTCTG ACAAATGCCT TATCTATTTC ATTGGCTGAT 
AAAACAATGG AATTCTTAGC AGGTGAAGAT ATGGATTTGA CTAAAAGTCA CACAACTAAG 
TTAAGTCAAG TTATTCCTAC AACTTTTGAC TTAGCATCAA AGAATGTCAC CAAATCTTCC 
TCTCACAGCA AAAGCCCTTC AGATGAGTGG GAAGGCCTGG AAAAACAAAT TGTGCTAGGC 
CAGCATTCTA AACTTCCTTC ACCACAGAGG AAAGATTTAG ATGATCCTGA CTGTTCTCAT 
CATAAGATAA TGTATTCAGA GGAGGTACAA ACTATGGATC TAACCAAGAG CCACACTATT 
GTGATTGGGT TTGGTTCTTC TGAGGTACAA GAACACAGTA AGATTAATTT AGAACACAAA 
AGCAGCCAAC TTACAGCAGA AAACAGACAA ACAGCTGTGA ACATTCCTGG AATAAACTTT 
AGAGTAGTAA CAACTAATGA TATGGACATG TTAAAGGACA GAAGTATACA TAAGCCAGAA 
TTGCTGGAGG AAAAGCAAAA CGTCAAAATT TATGGAAGAA AAAGTATGGG TGGACTGAAG 
ATTGATAAAA CTATATTATT TTCAGAGGGT AATGAGGATG ATATGGATAT CACTAAGAGT 
TGCACAGTCA AAATAAACCA TCGGTCTTTA TTAGATAAAC ATGATTCTCA TTTAGTATCT 
TTGGCAGGAA CTTCTAAAAC CATTTTGCAT GCACGTGGAC AGGATGAAAT GGAAATTAAT 
AGAAATCACA CGACTCCTTT AGAGTGTAAA ATTATCTCAC CCAGTGATAT AACTTCTGGT 
GATCTGAGTA AGACTGTGAT GTCTATAGAT GATCATGAGG AACTAGAAAT GACTAAGTCC 
CATACTGTTT TCATTGACTA CCAAGCAGAG GCAAAAGGTG TACTTCCAGA CAGACTTGAC 
TTTCAATTAT CCAAAAAGAA AAGCCTACAG AAGCCAAAAA TGACATCTAC TCATGCTGAA 
GAGATTGTTT CCATTTCAAA AAATAGTGAA AGCAACTATC TACCAGCCAA AGGCAGCCAG 
GTAACAATAT TAGAGGAAGG ATCTAATAGT GGGCCTGGGG AAGAAACTAA TGATGCACAA 
AAGCCTGGAT TTCTGAATGA ACCTCTGTCA GGCAAAACTC AAAGAAGAGA AAGCCTTAAG 
CTCAAAAATA AGACTATTAC ATTTTCAGGA AATGATAAAA GTAATAGAGA TATCACTCAG 
AGTTCTGTGG TAGAAATAAA TAATGAAACT AACCTGGAAG ATAGAAAGGG CTTCAGTTTT 
GTGCCATTGG CAGGAACTTC CAAGCCAGTT TTATCTGCAT ATCGGCCAGA GGACATGGAG 
ATCTCTATAA GTCAAACCTC TGCTTCGGAG TATAAAACTA TCCCACCAGA TGAAATGACT 
ACTATACCTA TGGATAAAAC TGTAATGTTT GTAGATAATC TCAGTGATCT GGGTGTCACC 
AGATCCCATA CGTTTTTCAT CGATTGTCAA GCCAAAGAGA AAGTACTTCA TGAATATACC 
AACTTGGGAA TACCAAAACC ACAAAACTTG AGTGGTTCTG AAGATAATTC CTGTATTCAA 
GAAATCACCA AAAAACCAGT AGCTGAATAC AAACACCATA TGACAACTGT TATTCCTTCT 
AATACATTAT TTAGTGATCA AAGTACAATG AAAATCAAGA TGCATAAAGC TGATATAGAT 
GAAGAGGGCA TAGGAAAGGA AGTGGAGGCC GATACATTGA AACAAACTAA ACTTGAAAGG 
TGTCTGTTAA ATAGAACTGA TGGAAAAAAT GTGGATTTTA CAAGTAGTCA TATAGCTGAT 
GTTTGTACAT CCAGAGACAA GTATTCCTCT TTACCAAATG TTATTTCTTC TTCTGATAAT 
TTAGATAGTA ATACTAAGTC CTTGTGTGAT AAAAGTAGGG AGAAAGCTTA TAATTGCCAA 
GTGCCAAATG AGTTTACTTT TGCAGATAAT TTACCAAGTA GATATCACCT GGATTCTGAA 
ATACCATCTG TCTTTGTATC ATGTCCTTCA AAAGAAGTTA CTCAAACTGA AAGTGCCATA 
GCACTACTTC AAGATCAAGA TCAAGAGCCT CTGGGACTCT CAAAGTGTGC ATCTCTTAAG 
TGTGCAAAGG ATCAAATGGA GGCCTTTGTT GATGTTAGTG TTGCTTCTCA GCCTCATCTC 
TCAGCCCAAC AACCTCCATC AACTCAAAAA GGGCAGGATG CCGCCAATAG AGATGAAGGA 
ATGCTATCTA AAGCTGGGAA GAAGACTTTA CCTTTTCTTA TAGGAAATGT CTCTGCACCC 
ACATGTGAAA ATGCGTCCAA AATGCCTACT AATGAGGAAC ACTTTGCTGT AACCTATAAC 
AAAGAACTGG AAAAAAGTAT TCAAACAGAT AAATGTAATA CAAATGTACA CAACCATAGT 
AACTCAGTCC TGACAACACA AGTCATTCAA ACACATGCCA GTGCCGAAGC AGCATCAGAC 
TCTATAGTTC CATCTAATGG TTCCTGTTTT AGTAGTGCCA AACCAAGTCT GAGTAATTTG 
AATAGAAAAA CTGGGAAGGT TTTAGACTTT AATACTGTTC ATATATTACC TCCTGCAGAA 
CAGTTACTTG AAGCAGGAAA TCAGGCACAT AGTATGAGTA TAGTACAAGG TACAGAAATA 
CATAGCCTAG GTTCCAGCAA TGACAGAGAT AAAGAAAGTA AAACTCTGCA TAATGAGGCC 
GAAGCTACAT CTGTACCACT AAAAGCAGTT GTTAAAGACA AAGCAAGAAG ATGTTCATTG 
GGAATATTTT TACCCAAATT ACCAAACAAG AAAAGTTGTA GTATCACTGG TGTTGATGAT 
GTGGAACAGA TTCTAGCAGA TGCAACTGAC TTAACTCACC TAGAAACTCA GCCAGTCTGT 
AGTCAAGATC CGGGCATTGG CTCTGTTGCA GCCAAACTGA ACTTGAGTCC ATCTCAGTAT 
ATAAATGAGG AGAATCTCCC TGTATATCCC GGTGAGATAC TTTCATCAGA TTCTGTCATC 
CTGGACATTG AGGAAAGTGC TCCGATCGAC ACATATCATA AAGAGATTTT ACCATCTGAA 
AACAAAACAG AAACCTGCAG GGCCCAAAAA AGAGCCTGGG TTCAAGAAAA TGAGGTTAAA 
AATGAGAAAA AAATCAGAAC ACATGATGGT GCACAAGATC AAGAGATTTT TGATCATTAT 
ACTGAAGAGG ACATCAATAA AAGTATTAAC AATGTATTTC TAAAAAGCCT GAGCAGGACA 
CCGTCTAGTT CTAGCAGCTC TCTGGATTCA ATCAAGGCTG ATGGAATCTC TCTGGATGCC 
AGCACACAGC GTAATAGCCA AATGGAATCA CAGTTTCTTG GTGACACTAT CTGTGAAGAG 
AGTTTGAGGG AGAAACTGAA GGATGGACAA ATTACAATCA AAGAGTTCTT CATACTTCTT 
CAAGTTCATA TCTTAATACA GAAACCTAGG CAGAGCACTC TCCCAGCCAA ATTTACTATA 
AACACATTAC CTACCACAGA AGATCTGATG TTGAGACAAT ATGTTTACGG ACCCAAGATA 
CAAATTTACA GAGAAGATTG TGAGCTTCTT CGACAGAAGA TTGAAGAATT GAAGGTATCT 
GCGTTGAACC AAGATAAACT ATTGGTGGAT GTAAATAGGA ACTTGTGGGA AAAAATGAAA 
GACTACTCTG ACGAAGAGCT GAAGACCTAT GGAATTTACC TTAACAAAAT AAAATCACGT 
TATACCAAGA TGACGAAAGT CTTCACTCAC CAAGGAAAAG TGGATCTGTA TAGCAAATTG 
GTGCACTCAG CTGAGACTGA GAAGAGGAAA CTTCAGATAA AAATCAACGA GATGGACACC 
ATACTTAAGA AGATCAATAA CTGTCTCACT GCAGTGGAAA TAGAAACTAA GAATTTGGAG 
GCTGAAGAGA AAGATGATGC CATGGAAGAT TGGGATTCTG AAATACGAGA TGCAGAGAAA 
GAACTGGAAA GGCTGAAAAC TGAGGAAGAG GAGCTTCAAA GAAAGTTCTT AGAAGTAGAG 
ACACAGAAAA CGCAGACCCT TGCTCAGATA GAATTTATAA AAGAACAAAC AACTGAAACA 
GAAGAATTAT TGGATCAATT GAGTTTTTCT GAGTGGGATA TCATCGAGTG GAGTGATGAA 
CAAGCGGTAT TTACCTTTGT TTATGATTCA ATAGAACTTA TCATCACTTT TGGAGAGCCT 
CAGGTTGGTC TCCCTTTCTT GGACAAGGCT TACAGGACGA TTAATGAACT CAGTTTTCAG 
TCTCTGTTAG ACGAAGATAA AGCGCCTCCT TCCTCCCTCT TGGTCCACAA GCTTATTTTC 
CAGTACATTG AAGAGCAGGA GTTCTGGAAG AAGAAGTGCA CAACACAGCA TCAGGTGCCC 
CAGATGCTTC AAGAACTCTC ATTGGTAGTT AACCATTGTA GGCTTCTTGG AGAAGAGATT 
GAATTTTTAA AGCGATGGGG ACCAAATTAT AGCCTAATGC ATATAAATGT GAATAATACT 
GAATTACGGC TTCTATTCTC CAGTTCTGCA GCATTTGCAA AGTTTGAGAT AACTTTGTTT 
CCCTCGGCCC ATTATCCCTT GGTCCCACTA CCATTCACCA TTCAAAATCA CCTTGGGAAG 
ACTGGCCATG ATGAAATTGC TGCCGTTATC TCTAAAGTGC CCTTAGAGGA AAACTACCTG 
AAGAATGTAG TCAAGCAGAT TTACCAAGAT CTACTTAAGG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Knl1 ( XM_008762095.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008760317.1 Rattus norvegicus kinetochore scaffold 1 (Knl1), transcript variant X1, mRNA.

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CloneID	ORa26224
Clone ID Related Accession (Same CDS sequence)	XM_008762095.1
Accession Version	XM_008762095.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6471bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	protein CASC5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGAGGA GCGCGCGGAC CCCAGACAAG TCAAGCAGAA AAACGTTTTC AAAAATGGAT 
GGGATATATT CAGAGGCTAA TGAAGAGAAT GACAATACAC AGAGGCCTGT TAGAAGACAG 
CATTCCTCAA TTTTGAAACC CCCAAGGAGT CCTCTTCAGG ACCTCAAATG TGGAAATGAA 
ATAGTTCAGG AATCTAATGC TCCGAGAACC AGGAAAAGCT CTCGTCGGGT CAGCTTTGCA 
GATACTATAA AAGTATTCCA GACAGAGTCC CATATGAAAA CGGAAAAAAA TTCAGAAATT 
TCAGAGACAG AAGCCAGAAA AAATGTGTTG ACCCTACAGA ACAAGAATTT AGATGATAAC 
TATTGTGCAA TTACTGGAAT GAACACTTTG CTTTGTGCTC CAATTCAGAC TCAGATGCAA 
CAAAAGGAGT TTTCAATTAC AGACTGCAAC CCTGAAAGGA AACATGCAAA TGATCAAACA 
GTCATCTTTT CAGATGAAAA TCAGATGGAT CTGACAGCAA GTCACACGGT GATGATTGCC 
AAAGGTCTTG TAGACTGTAC CAAACATGAA AATTCCACAA AAATAGATAC CACATCCTTT 
CTGGAGAATT TAAAGCATCG TGCTGCGAAT TCAAGAGTTA AAAACGATTT AGCATGTCCA 
ATAATTTCTA TAAATCAAAA CATTTTTTCA GAAAAGAAAA ATTCTGACAA CTTCATAAAA 
AGATTGAAAA TGGGGAAATA TAATACTTCT TCTACAGAAC TTGATAAGGA AAATGCTGAA 
ATACCTGTTC ATTCCAAAGA CTCAAACAGT GCCTCTTCCA CGTGTCAAAT ACATGCATCG 
CTTAGTGTCG ATGAACATAG TAGTAATAAG ACTAGACTCT TTACAGAACA AGATGATGGG 
ATGAATTTGA CCCAGTGTCA TACAGCCTGT ATTCAGACTT GGATTCCTCC ATCCAGGGAG 
GCCACCTTGG GAGAGGTTAA AGGTGATAAT ACAATTTATG GCAATGAGTG TATGGAGTTG 
ACAACTAACT ACACGATACA GGTGTTGTCA TCAGAAAATA ACTTACCTGA AAGAGAAAAT 
CAAGCTCAAA ATGATGTGAT TGATGTTACA ACAGTCGATG GAGCTACAGC TCCAGAGAAA 
AAAACAGCCC TTAAGGATAA ACTAAATGCT GCTTTTCACA GCTCTTTCCC AAACCCTGAA 
AATAAACTTC ATATTACCAA ATGTCATCCT ATAGAGTCAG AAACTCATAC AGTCACACAG 
ATTTCTAATC AAAATGCCAA CACATTGGCT ATGACCTCAG AATCTGTATG TTCTAGCCCA 
GCTATTCAAG GGTATAAAAC AATTTTTTAC TCTAGTAGCA ATGATGCAAT GGAACTGACT 
AAGTGTCTAT CAATTATGCA AGAGGAAAAA AAATTGCTGA AGACTGATGA TAAGTATTCT 
AAAATATGTA CCAATTCAGA TGCTAGCCCA CTCAGAGAGA AAACTATTTA TTCAGAAGAG 
GATAGCATGG ACATTACCAA AAGTCACACA GTTGCAATAG ATAATAAGAT ATTTAAACAT 
GATCAAGAAA ACATTAAAAA AGAAATAACA GCTCTACCAA TATTTGAAAA AGAAATGATG 
CTCCAAAATC TTAAAACTAT GTCCAAAGAT GAGAAAATGA ATGTGAATTC TATTACAGTT 
CCTCAAGTAT CTAAAGAAAG TTTACAACAG AGTCTGACAA ATGCCTTATC TATTTCATTG 
GCTGATAAAA CAATGGAATT CTTAGCAGGT GAAGATATGG ATTTGACTAA AAGTCACACA 
ACTAAGTTAA GTCAAGTTAT TCCTACAACT TTTGACTTAG CATCAAAGAA TGTCACCAAA 
TCTTCCTCTC ACAGCAAAAG CCCTTCAGAT GAGTGGGAAG GCCTGGAAAA ACAAATTGTG 
CTAGGCCAGC ATTCTAAACT TCCTTCACCA CAGAGGAAAG ATTTAGATGA TCCTGACTGT 
TCTCATCATA AGATAATGTA TTCAGAGGAG GTACAAACTA TGGATCTAAC CAAGAGCCAC 
ACTATTGTGA TTGGGTTTGG TTCTTCTGAG GTACAAGAAC ACAGTAAGAT TAATTTAGAA 
CACAAAAGCA GCCAACTTAC AGCAGAAAAC AGACAAACAG CTGTGAACAT TCCTGGAATA 
AACTTTAGAG TAGTAACAAC TAATGATATG GACATGTTAA AGGACAGAAG TATACATAAG 
CCAGAATTGC TGGAGGAAAA GCAAAACGTC AAAATTTATG GAAGAAAAAG TATGGGTGGA 
CTGAAGATTG ATAAAACTAT ATTATTTTCA GAGGGTAATG AGGATGATAT GGATATCACT 
AAGAGTTGCA CAGTCAAAAT AAACCATCGG TCTTTATTAG ATAAACATGA TTCTCATTTA 
GTATCTTTGG CAGGAACTTC TAAAACCATT TTGCATGCAC GTGGACAGGA TGAAATGGAA 
ATTAATAGAA ATCACACGAC TCCTTTAGAG TGTAAAATTA TCTCACCCAG TGATATAACT 
TCTGGTGATC TGAGTAAGAC TGTGATGTCT ATAGATGATC ATGAGGAACT AGAAATGACT 
AAGTCCCATA CTGTTTTCAT TGACTACCAA GCAGAGGCAA AAGGTGTACT TCCAGACAGA 
CTTGACTTTC AATTATCCAA AAAGAAAAGC CTACAGAAGC CAAAAATGAC ATCTACTCAT 
GCTGAAGAGA TTGTTTCCAT TTCAAAAAAT AGTGAAAGCA ACTATCTACC AGCCAAAGGC 
AGCCAGGTAA CAATATTAGA GGAAGGATCT AATAGTGGGC CTGGGGAAGA AACTAATGAT 
GCACAAAAGC CTGGATTTCT GAATGAACCT CTGTCAGGCA AAACTCAAAG AAGAGAAAGC 
CTTAAGCTCA AAAATAAGAC TATTACATTT TCAGGAAATG ATAAAAGTAA TAGAGATATC 
ACTCAGAGTT CTGTGGTAGA AATAAATAAT GAAACTAACC TGGAAGATAG AAAGGGCTTC 
AGTTTTGTGC CATTGGCAGG AACTTCCAAG CCAGTTTTAT CTGCATATCG GCCAGAGGAC 
ATGGAGATCT CTATAAGTCA AACCTCTGCT TCGGAGTATA AAACTATCCC ACCAGATGAA 
ATGACTACTA TACCTATGGA TAAAACTGTA ATGTTTGTAG ATAATCTCAG TGATCTGGGT 
GTCACCAGAT CCCATACGTT TTTCATCGAT TGTCAAGCCA AAGAGAAAGT ACTTCATGAA 
TATACCAACT TGGGAATACC AAAACCACAA AACTTGAGTG GTTCTGAAGA TAATTCCTGT 
ATTCAAGAAA TCACCAAAAA ACCAGTAGCT GAATACAAAC ACCATATGAC AACTGTTATT 
CCTTCTAATA CATTATTTAG TGATCAAAGT ACAATGAAAA TCAAGATGCA TAAAGCTGAT 
ATAGATGAAG AGGGCATAGG AAAGGAAGTG GAGGCCGATA CATTGAAACA AACTAAACTT 
GAAAGGTGTC TGTTAAATAG AACTGATGGA AAAAATGTGG ATTTTACAAG TAGTCATATA 
GCTGATGTTT GTACATCCAG AGACAAGTAT TCCTCTTTAC CAAATGTTAT TTCTTCTTCT 
GATAATTTAG ATAGTAATAC TAAGTCCTTG TGTGATAAAA GTAGGGAGAA AGCTTATAAT 
TGCCAAGTGC CAAATGAGTT TACTTTTGCA GATAATTTAC CAAGTAGATA TCACCTGGAT 
TCTGAAATAC CATCTGTCTT TGTATCATGT CCTTCAAAAG AAGTTACTCA AACTGAAAGT 
GCCATAGCAC TACTTCAAGA TCAAGATCAA GAGCCTCTGG GACTCTCAAA GTGTGCATCT 
CTTAAGTGTG CAAAGGATCA AATGGAGGCC TTTGTTGATG TTAGTGTTGC TTCTCAGCCT 
CATCTCTCAG CCCAACAACC TCCATCAACT CAAAAAGGGC AGGATGCCGC CAATAGAGAT 
GAAGGAATGC TATCTAAAGC TGGGAAGAAG ACTTTACCTT TTCTTATAGG AAATGTCTCT 
GCACCCACAT GTGAAAATGC GTCCAAAATG CCTACTAATG AGGAACACTT TGCTGTAACC 
TATAACAAAG AACTGGAAAA AAGTATTCAA ACAGATAAAT GTAATACAAA TGTACACAAC 
CATAGTAACT CAGTCCTGAC AACACAAGTC ATTCAAACAC ATGCCAGTGC CGAAGCAGCA 
TCAGACTCTA TAGTTCCATC TAATGGTTCC TGTTTTAGTA GTGCCAAACC AAGTCTGAGT 
AATTTGAATA GAAAAACTGG GAAGGTTTTA GACTTTAATA CTGTTCATAT ATTACCTCCT 
GCAGAACAGT TACTTGAAGC AGGAAATCAG GCACATAGTA TGAGTATAGT ACAAGGTACA 
GAAATACATA GCCTAGGTTC CAGCAATGAC AGAGATAAAG AAAGTAAAAC TCTGCATAAT 
GAGGCCGAAG CTACATCTGT ACCACTAAAA GCAGTTGTTA AAGACAAAGC AAGAAGATGT 
TCATTGGGAA TATTTTTACC CAAATTACCA AACAAGAAAA GTTGTAGTAT CACTGGTGTT 
GATGATGTGG AACAGATTCT AGCAGATGCA ACTGACTTAA CTCACCTAGA AACTCAGCCA 
GTCTGTAGTC AAGATCCGGG CATTGGCTCT GTTGCAGCCA AACTGAACTT GAGTCCATCT 
CAGTATATAA ATGAGGAGAA TCTCCCTGTA TATCCCGGTG AGATACTTTC ATCAGATTCT 
GTCATCCTGG ACATTGAGGA AAGTGCTCCG ATCGACACAT ATCATAAAGA GATTTTACCA 
TCTGAAAACA AAACAGAAAC CTGCAGGGCC CAAAAAAGAG CCTGGGTTCA AGAAAATGAG 
GTTAAAAATG AGAAAAAAAT CAGAACACAT GATGGTGCAC AAGATCAAGA GATTTTTGAT 
CATTATACTG AAGAGGACAT CAATAAAAGT ATTAACAATG TATTTCTAAA AAGCCTGAGC 
AGGACACCGT CTAGTTCTAG CAGCTCTCTG GATTCAATCA AGGCTGATGG AATCTCTCTG 
GATGCCAGCA CACAGCGTAA TAGCCAAATG GAATCACAGT TTCTTGGTGA CACTATCTGT 
GAAGAGAGTT TGAGGGAGAA ACTGAAGGAT GGACAAATTA CAATCAAAGA GTTCTTCATA 
CTTCTTCAAG TTCATATCTT AATACAGAAA CCTAGGCAGA GCACTCTCCC AGCCAAATTT 
ACTATAAACA CATTACCTAC CACAGAAGAT CTGATGTTGA GACAATATGT TTACGGACCC 
AAGATACAAA TTTACAGAGA AGATTGTGAG CTTCTTCGAC AGAAGATTGA AGAATTGAAG 
GTATCTGCGT TGAACCAAGA TAAACTATTG GTGGATGTAA ATAGGAACTT GTGGGAAAAA 
ATGAAAGACT ACTCTGACGA AGAGCTGAAG ACCTATGGAA TTTACCTTAA CAAAATAAAA 
TCACGTTATA CCAAGATGAC GAAAGTCTTC ACTCACCAAG GAAAAGTGGA TCTGTATAGC 
AAATTGGTGC ACTCAGCTGA GACTGAGAAG AGGAAACTTC AGATAAAAAT CAACGAGATG 
GACACCATAC TTAAGAAGAT CAATAACTGT CTCACTGCAG TGGAAATAGA AACTAAGAAT 
TTGGAGGCTG AAGAGAAAGA TGATGCCATG GAAGATTGGG ATTCTGAAAT ACGAGATGCA 
GAGAAAGAAC TGGAAAGGCT GAAAACTGAG GAAGAGGAGC TTCAAAGAAA GTTCTTAGAA 
GTAGAGACAC AGAAAACGCA GACCCTTGCT CAGATAGAAT TTATAAAAGA ACAAACAACT 
GAAACAGAAG AATTATTGGA TCAATTGAGT TTTTCTGAGT GGGATATCAT CGAGTGGAGT 
GATGAACAAG CGGTATTTAC CTTTGTTTAT GATTCAATAG AACTTATCAT CACTTTTGGA 
GAGCCTCAGG TTGGTCTCCC TTTCTTGGAC AAGGCTTACA GGACGATTAA TGAACTCAGT 
TTTCAGTCTC TGTTAGACGA AGATAAAGCG CCTCCTTCCT CCCTCTTGGT CCACAAGCTT 
ATTTTCCAGT ACATTGAAGA GCAGGAGTTC TGGAAGAAGA AGTGCACAAC ACAGCATCAG 
GTGCCCCAGA TGCTTCAAGA ACTCTCATTG GTAGTTAACC ATTGTAGGCT TCTTGGAGAA 
GAGATTGAAT TTTTAAAGCG ATGGGGACCA AATTATAGCC TAATGCATAT AAATGTGAAT 
AATACTGAAT TACGGCTTCT ATTCTCCAGT TCTGCAGCAT TTGCAAAGTT TGAGATAACT 
TTGTTTCCCT CGGCCCATTA TCCCTTGGTC CCACTACCAT TCACCATTCA AAATCACCTT 
GGGAAGACTG GCCATGATGA AATTGCTGCC GTTATCTCTA AAGTGCCCTT AGAGGAAAAC 
TACCTGAAGA ATGTAGTCAA GCAGATTTAC CAAGATCTAC TTAAGGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008760317.1
CDS	61..6531
Translation	MRRSARTPDKSSRKTFSKMDGIYSEANEENDNTQRPVRRQHSSILKPPRSPLQDLKCGNEIVQESNAPRTRKSSRRVSFADTIKVFQTESHMKTEKNSEISETEARKNVLTLQNKNLDDNYCAITGMNTLLCAPIQTQMQQKEFSITDCNPERKHANDQTVIFSDENQMDLTASHTVMIAKGLVDCTKHENSTKIDTTSFLENLKHRAANSRVKNDLACPIISINQNIFSEKKNSDNFIKRLKMGKYNTSSTELDKENAEIPVHSKDSNSASSTCQIHASLSVDEHSSNKTRLFTEQDDGMNLTQCHTACIQTWIPPSREATLGEVKGDNTIYGNECMELTTNYTIQVLSSENNLPERENQAQNDVIDVTTVDGATAPEKKTALKDKLNAAFHSSFPNPENKLHITKCHPIESETHTVTQISNQNANTLAMTSESVCSSPAIQGYKTIFYSSSNDAMELTKCLSIMQEEKKLLKTDDKYSKICTNSDASPLREKTIYSEEDSMDITKSHTVAIDNKIFKHDQENIKKEITALPIFEKEMMLQNLKTMSKDEKMNVNSITVPQVSKESLQQSLTNALSISLADKTMEFLAGEDMDLTKSHTTKLSQVIPTTFDLASKNVTKSSSHSKSPSDEWEGLEKQIVLGQHSKLPSPQRKDLDDPDCSHHKIMYSEEVQTMDLTKSHTIVIGFGSSEVQEHSKINLEHKSSQLTAENRQTAVNIPGINFRVVTTNDMDMLKDRSIHKPELLEEKQNVKIYGRKSMGGLKIDKTILFSEGNEDDMDITKSCTVKINHRSLLDKHDSHLVSLAGTSKTILHARGQDEMEINRNHTTPLECKIISPSDITSGDLSKTVMSIDDHEELEMTKSHTVFIDYQAEAKGVLPDRLDFQLSKKKSLQKPKMTSTHAEEIVSISKNSESNYLPAKGSQVTILEEGSNSGPGEETNDAQKPGFLNEPLSGKTQRRESLKLKNKTITFSGNDKSNRDITQSSVVEINNETNLEDRKGFSFVPLAGTSKPVLSAYRPEDMEISISQTSASEYKTIPPDEMTTIPMDKTVMFVDNLSDLGVTRSHTFFIDCQAKEKVLHEYTNLGIPKPQNLSGSEDNSCIQEITKKPVAEYKHHMTTVIPSNTLFSDQSTMKIKMHKADIDEEGIGKEVEADTLKQTKLERCLLNRTDGKNVDFTSSHIADVCTSRDKYSSLPNVISSSDNLDSNTKSLCDKSREKAYNCQVPNEFTFADNLPSRYHLDSEIPSVFVSCPSKEVTQTESAIALLQDQDQEPLGLSKCASLKCAKDQMEAFVDVSVASQPHLSAQQPPSTQKGQDAANRDEGMLSKAGKKTLPFLIGNVSAPTCENASKMPTNEEHFAVTYNKELEKSIQTDKCNTNVHNHSNSVLTTQVIQTHASAEAASDSIVPSNGSCFSSAKPSLSNLNRKTGKVLDFNTVHILPPAEQLLEAGNQAHSMSIVQGTEIHSLGSSNDRDKESKTLHNEAEATSVPLKAVVKDKARRCSLGIFLPKLPNKKSCSITGVDDVEQILADATDLTHLETQPVCSQDPGIGSVAAKLNLSPSQYINEENLPVYPGEILSSDSVILDIEESAPIDTYHKEILPSENKTETCRAQKRAWVQENEVKNEKKIRTHDGAQDQEIFDHYTEEDINKSINNVFLKSLSRTPSSSSSSLDSIKADGISLDASTQRNSQMESQFLGDTICEESLREKLKDGQITIKEFFILLQVHILIQKPRQSTLPAKFTINTLPTTEDLMLRQYVYGPKIQIYREDCELLRQKIEELKVSALNQDKLLVDVNRNLWEKMKDYSDEELKTYGIYLNKIKSRYTKMTKVFTHQGKVDLYSKLVHSAETEKRKLQIKINEMDTILKKINNCLTAVEIETKNLEAEEKDDAMEDWDSEIRDAEKELERLKTEEEELQRKFLEVETQKTQTLAQIEFIKEQTTETEELLDQLSFSEWDIIEWSDEQAVFTFVYDSIELIITFGEPQVGLPFLDKAYRTINELSFQSLLDEDKAPPSSLLVHKLIFQYIEEQEFWKKKCTTQHQVPQMLQELSLVVNHCRLLGEEIEFLKRWGPNYSLMHINVNNTELRLLFSSSAAFAKFEITLFPSAHYPLVPLPFTIQNHLGKTGHDEIAAVISKVPLEENYLKNVVKQIYQDLLKD

Target ORF information:

RefSeq Version	XM_008762095.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus kinetochore scaffold 1 (Knl1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008762095.1

ORF Insert Sequence:

ATGCGGAGGA GCGCGCGGAC CCCAGACAAG TCAAGCAGAA AAACGTTTTC AAAAATGGAT 
GGGATATATT CAGAGGCTAA TGAAGAGAAT GACAATACAC AGAGGCCTGT TAGAAGACAG 
CATTCCTCAA TTTTGAAACC CCCAAGGAGT CCTCTTCAGG ACCTCAAATG TGGAAATGAA 
ATAGTTCAGG AATCTAATGC TCCGAGAACC AGGAAAAGCT CTCGTCGGGT CAGCTTTGCA 
GATACTATAA AAGTATTCCA GACAGAGTCC CATATGAAAA CGGAAAAAAA TTCAGAAATT 
TCAGAGACAG AAGCCAGAAA AAATGTGTTG ACCCTACAGA ACAAGAATTT AGATGATAAC 
TATTGTGCAA TTACTGGAAT GAACACTTTG CTTTGTGCTC CAATTCAGAC TCAGATGCAA 
CAAAAGGAGT TTTCAATTAC AGACTGCAAC CCTGAAAGGA AACATGCAAA TGATCAAACA 
GTCATCTTTT CAGATGAAAA TCAGATGGAT CTGACAGCAA GTCACACGGT GATGATTGCC 
AAAGGTCTTG TAGACTGTAC CAAACATGAA AATTCCACAA AAATAGATAC CACATCCTTT 
CTGGAGAATT TAAAGCATCG TGCTGCGAAT TCAAGAGTTA AAAACGATTT AGCATGTCCA 
ATAATTTCTA TAAATCAAAA CATTTTTTCA GAAAAGAAAA ATTCTGACAA CTTCATAAAA 
AGATTGAAAA TGGGGAAATA TAATACTTCT TCTACAGAAC TTGATAAGGA AAATGCTGAA 
ATACCTGTTC ATTCCAAAGA CTCAAACAGT GCCTCTTCCA CGTGTCAAAT ACATGCATCG 
CTTAGTGTCG ATGAACATAG TAGTAATAAG ACTAGACTCT TTACAGAACA AGATGATGGG 
ATGAATTTGA CCCAGTGTCA TACAGCCTGT ATTCAGACTT GGATTCCTCC ATCCAGGGAG 
GCCACCTTGG GAGAGGTTAA AGGTGATAAT ACAATTTATG GCAATGAGTG TATGGAGTTG 
ACAACTAACT ACACGATACA GGTGTTGTCA TCAGAAAATA ACTTACCTGA AAGAGAAAAT 
CAAGCTCAAA ATGATGTGAT TGATGTTACA ACAGTCGATG GAGCTACAGC TCCAGAGAAA 
AAAACAGCCC TTAAGGATAA ACTAAATGCT GCTTTTCACA GCTCTTTCCC AAACCCTGAA 
AATAAACTTC ATATTACCAA ATGTCATCCT ATAGAGTCAG AAACTCATAC AGTCACACAG 
ATTTCTAATC AAAATGCCAA CACATTGGCT ATGACCTCAG AATCTGTATG TTCTAGCCCA 
GCTATTCAAG GGTATAAAAC AATTTTTTAC TCTAGTAGCA ATGATGCAAT GGAACTGACT 
AAGTGTCTAT CAATTATGCA AGAGGAAAAA AAATTGCTGA AGACTGATGA TAAGTATTCT 
AAAATATGTA CCAATTCAGA TGCTAGCCCA CTCAGAGAGA AAACTATTTA TTCAGAAGAG 
GATAGCATGG ACATTACCAA AAGTCACACA GTTGCAATAG ATAATAAGAT ATTTAAACAT 
GATCAAGAAA ACATTAAAAA AGAAATAACA GCTCTACCAA TATTTGAAAA AGAAATGATG 
CTCCAAAATC TTAAAACTAT GTCCAAAGAT GAGAAAATGA ATGTGAATTC TATTACAGTT 
CCTCAAGTAT CTAAAGAAAG TTTACAACAG AGTCTGACAA ATGCCTTATC TATTTCATTG 
GCTGATAAAA CAATGGAATT CTTAGCAGGT GAAGATATGG ATTTGACTAA AAGTCACACA 
ACTAAGTTAA GTCAAGTTAT TCCTACAACT TTTGACTTAG CATCAAAGAA TGTCACCAAA 
TCTTCCTCTC ACAGCAAAAG CCCTTCAGAT GAGTGGGAAG GCCTGGAAAA ACAAATTGTG 
CTAGGCCAGC ATTCTAAACT TCCTTCACCA CAGAGGAAAG ATTTAGATGA TCCTGACTGT 
TCTCATCATA AGATAATGTA TTCAGAGGAG GTACAAACTA TGGATCTAAC CAAGAGCCAC 
ACTATTGTGA TTGGGTTTGG TTCTTCTGAG GTACAAGAAC ACAGTAAGAT TAATTTAGAA 
CACAAAAGCA GCCAACTTAC AGCAGAAAAC AGACAAACAG CTGTGAACAT TCCTGGAATA 
AACTTTAGAG TAGTAACAAC TAATGATATG GACATGTTAA AGGACAGAAG TATACATAAG 
CCAGAATTGC TGGAGGAAAA GCAAAACGTC AAAATTTATG GAAGAAAAAG TATGGGTGGA 
CTGAAGATTG ATAAAACTAT ATTATTTTCA GAGGGTAATG AGGATGATAT GGATATCACT 
AAGAGTTGCA CAGTCAAAAT AAACCATCGG TCTTTATTAG ATAAACATGA TTCTCATTTA 
GTATCTTTGG CAGGAACTTC TAAAACCATT TTGCATGCAC GTGGACAGGA TGAAATGGAA 
ATTAATAGAA ATCACACGAC TCCTTTAGAG TGTAAAATTA TCTCACCCAG TGATATAACT 
TCTGGTGATC TGAGTAAGAC TGTGATGTCT ATAGATGATC ATGAGGAACT AGAAATGACT 
AAGTCCCATA CTGTTTTCAT TGACTACCAA GCAGAGGCAA AAGGTGTACT TCCAGACAGA 
CTTGACTTTC AATTATCCAA AAAGAAAAGC CTACAGAAGC CAAAAATGAC ATCTACTCAT 
GCTGAAGAGA TTGTTTCCAT TTCAAAAAAT AGTGAAAGCA ACTATCTACC AGCCAAAGGC 
AGCCAGGTAA CAATATTAGA GGAAGGATCT AATAGTGGGC CTGGGGAAGA AACTAATGAT 
GCACAAAAGC CTGGATTTCT GAATGAACCT CTGTCAGGCA AAACTCAAAG AAGAGAAAGC 
CTTAAGCTCA AAAATAAGAC TATTACATTT TCAGGAAATG ATAAAAGTAA TAGAGATATC 
ACTCAGAGTT CTGTGGTAGA AATAAATAAT GAAACTAACC TGGAAGATAG AAAGGGCTTC 
AGTTTTGTGC CATTGGCAGG AACTTCCAAG CCAGTTTTAT CTGCATATCG GCCAGAGGAC 
ATGGAGATCT CTATAAGTCA AACCTCTGCT TCGGAGTATA AAACTATCCC ACCAGATGAA 
ATGACTACTA TACCTATGGA TAAAACTGTA ATGTTTGTAG ATAATCTCAG TGATCTGGGT 
GTCACCAGAT CCCATACGTT TTTCATCGAT TGTCAAGCCA AAGAGAAAGT ACTTCATGAA 
TATACCAACT TGGGAATACC AAAACCACAA AACTTGAGTG GTTCTGAAGA TAATTCCTGT 
ATTCAAGAAA TCACCAAAAA ACCAGTAGCT GAATACAAAC ACCATATGAC AACTGTTATT 
CCTTCTAATA CATTATTTAG TGATCAAAGT ACAATGAAAA TCAAGATGCA TAAAGCTGAT 
ATAGATGAAG AGGGCATAGG AAAGGAAGTG GAGGCCGATA CATTGAAACA AACTAAACTT 
GAAAGGTGTC TGTTAAATAG AACTGATGGA AAAAATGTGG ATTTTACAAG TAGTCATATA 
GCTGATGTTT GTACATCCAG AGACAAGTAT TCCTCTTTAC CAAATGTTAT TTCTTCTTCT 
GATAATTTAG ATAGTAATAC TAAGTCCTTG TGTGATAAAA GTAGGGAGAA AGCTTATAAT 
TGCCAAGTGC CAAATGAGTT TACTTTTGCA GATAATTTAC CAAGTAGATA TCACCTGGAT 
TCTGAAATAC CATCTGTCTT TGTATCATGT CCTTCAAAAG AAGTTACTCA AACTGAAAGT 
GCCATAGCAC TACTTCAAGA TCAAGATCAA GAGCCTCTGG GACTCTCAAA GTGTGCATCT 
CTTAAGTGTG CAAAGGATCA AATGGAGGCC TTTGTTGATG TTAGTGTTGC TTCTCAGCCT 
CATCTCTCAG CCCAACAACC TCCATCAACT CAAAAAGGGC AGGATGCCGC CAATAGAGAT 
GAAGGAATGC TATCTAAAGC TGGGAAGAAG ACTTTACCTT TTCTTATAGG AAATGTCTCT 
GCACCCACAT GTGAAAATGC GTCCAAAATG CCTACTAATG AGGAACACTT TGCTGTAACC 
TATAACAAAG AACTGGAAAA AAGTATTCAA ACAGATAAAT GTAATACAAA TGTACACAAC 
CATAGTAACT CAGTCCTGAC AACACAAGTC ATTCAAACAC ATGCCAGTGC CGAAGCAGCA 
TCAGACTCTA TAGTTCCATC TAATGGTTCC TGTTTTAGTA GTGCCAAACC AAGTCTGAGT 
AATTTGAATA GAAAAACTGG GAAGGTTTTA GACTTTAATA CTGTTCATAT ATTACCTCCT 
GCAGAACAGT TACTTGAAGC AGGAAATCAG GCACATAGTA TGAGTATAGT ACAAGGTACA 
GAAATACATA GCCTAGGTTC CAGCAATGAC AGAGATAAAG AAAGTAAAAC TCTGCATAAT 
GAGGCCGAAG CTACATCTGT ACCACTAAAA GCAGTTGTTA AAGACAAAGC AAGAAGATGT 
TCATTGGGAA TATTTTTACC CAAATTACCA AACAAGAAAA GTTGTAGTAT CACTGGTGTT 
GATGATGTGG AACAGATTCT AGCAGATGCA ACTGACTTAA CTCACCTAGA AACTCAGCCA 
GTCTGTAGTC AAGATCCGGG CATTGGCTCT GTTGCAGCCA AACTGAACTT GAGTCCATCT 
CAGTATATAA ATGAGGAGAA TCTCCCTGTA TATCCCGGTG AGATACTTTC ATCAGATTCT 
GTCATCCTGG ACATTGAGGA AAGTGCTCCG ATCGACACAT ATCATAAAGA GATTTTACCA 
TCTGAAAACA AAACAGAAAC CTGCAGGGCC CAAAAAAGAG CCTGGGTTCA AGAAAATGAG 
GTTAAAAATG AGAAAAAAAT CAGAACACAT GATGGTGCAC AAGATCAAGA GATTTTTGAT 
CATTATACTG AAGAGGACAT CAATAAAAGT ATTAACAATG TATTTCTAAA AAGCCTGAGC 
AGGACACCGT CTAGTTCTAG CAGCTCTCTG GATTCAATCA AGGCTGATGG AATCTCTCTG 
GATGCCAGCA CACAGCGTAA TAGCCAAATG GAATCACAGT TTCTTGGTGA CACTATCTGT 
GAAGAGAGTT TGAGGGAGAA ACTGAAGGAT GGACAAATTA CAATCAAAGA GTTCTTCATA 
CTTCTTCAAG TTCATATCTT AATACAGAAA CCTAGGCAGA GCACTCTCCC AGCCAAATTT 
ACTATAAACA CATTACCTAC CACAGAAGAT CTGATGTTGA GACAATATGT TTACGGACCC 
AAGATACAAA TTTACAGAGA AGATTGTGAG CTTCTTCGAC AGAAGATTGA AGAATTGAAG 
GTATCTGCGT TGAACCAAGA TAAACTATTG GTGGATGTAA ATAGGAACTT GTGGGAAAAA 
ATGAAAGACT ACTCTGACGA AGAGCTGAAG ACCTATGGAA TTTACCTTAA CAAAATAAAA 
TCACGTTATA CCAAGATGAC GAAAGTCTTC ACTCACCAAG GAAAAGTGGA TCTGTATAGC 
AAATTGGTGC ACTCAGCTGA GACTGAGAAG AGGAAACTTC AGATAAAAAT CAACGAGATG 
GACACCATAC TTAAGAAGAT CAATAACTGT CTCACTGCAG TGGAAATAGA AACTAAGAAT 
TTGGAGGCTG AAGAGAAAGA TGATGCCATG GAAGATTGGG ATTCTGAAAT ACGAGATGCA 
GAGAAAGAAC TGGAAAGGCT GAAAACTGAG GAAGAGGAGC TTCAAAGAAA GTTCTTAGAA 
GTAGAGACAC AGAAAACGCA GACCCTTGCT CAGATAGAAT TTATAAAAGA ACAAACAACT 
GAAACAGAAG AATTATTGGA TCAATTGAGT TTTTCTGAGT GGGATATCAT CGAGTGGAGT 
GATGAACAAG CGGTATTTAC CTTTGTTTAT GATTCAATAG AACTTATCAT CACTTTTGGA 
GAGCCTCAGG TTGGTCTCCC TTTCTTGGAC AAGGCTTACA GGACGATTAA TGAACTCAGT 
TTTCAGTCTC TGTTAGACGA AGATAAAGCG CCTCCTTCCT CCCTCTTGGT CCACAAGCTT 
ATTTTCCAGT ACATTGAAGA GCAGGAGTTC TGGAAGAAGA AGTGCACAAC ACAGCATCAG 
GTGCCCCAGA TGCTTCAAGA ACTCTCATTG GTAGTTAACC ATTGTAGGCT TCTTGGAGAA 
GAGATTGAAT TTTTAAAGCG ATGGGGACCA AATTATAGCC TAATGCATAT AAATGTGAAT 
AATACTGAAT TACGGCTTCT ATTCTCCAGT TCTGCAGCAT TTGCAAAGTT TGAGATAACT 
TTGTTTCCCT CGGCCCATTA TCCCTTGGTC CCACTACCAT TCACCATTCA AAATCACCTT 
GGGAAGACTG GCCATGATGA AATTGCTGCC GTTATCTCTA AAGTGCCCTT AGAGGAAAAC 
TACCTGAAGA ATGTAGTCAA GCAGATTTAC CAAGATCTAC TTAAGGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Knl1 ( XM_008762099.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008760321.1 Rattus norvegicus kinetochore scaffold 1 (Knl1), transcript variant X5, mRNA.

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CloneID	ORa46967
Clone ID Related Accession (Same CDS sequence)	XM_008762099.2 , XM_008762097.2 , XM_008762096.1
Accession Version	XM_008762099.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6417bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	protein CASC5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008762099.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGGGA TATATTCAGA GGCTAATGAA GAGAATGACA ATACACAGAG GCCTGTTAGA 
AGACAGCATT CCTCAATTTT GAAACCCCCA AGGAGTCCTC TTCAGGACCT CAAATGTGGA 
AATGAAATAG TTCAGGAATC TAATGCTCCG AGAACCAGGA AAAGCTCTCG TCGGGTCAGC 
TTTGCAGATA CTATAAAAGT ATTCCAGACA GAGTCCCATA TGAAAACGGA AAAAAATTCA 
GAAATTTCAG AGACAGAAGC CAGAAAAAAT GTGTTGACCC TACAGAACAA GAATTTAGAT 
GATAACTATT GTGCAATTAC TGGAATGAAC ACTTTGCTTT GTGCTCCAAT TCAGACTCAG 
ATGCAACAAA AGGAGTTTTC AATTACAGAC TGCAACCCTG AAAGGAAACA TGCAAATGAT 
CAAACAGTCA TCTTTTCAGA TGAAAATCAG ATGGATCTGA CAGCAAGTCA CACGGTGATG 
ATTGCCAAAG GTCTTGTAGA CTGTACCAAA CATGAAAATT CCACAAAAAT AGATACCACA 
TCCTTTCTGG AGAATTTAAA GCATCGTGCT GCGAATTCAA GAGTTAAAAA CGATTTAGCA 
TGTCCAATAA TTTCTATAAA TCAAAACATT TTTTCAGAAA AGAAAAATTC TGACAACTTC 
ATAAAAAGAT TGAAAATGGG GAAATATAAT ACTTCTTCTA CAGAACTTGA TAAGGAAAAT 
GCTGAAATAC CTGTTCATTC CAAAGACTCA AACAGTGCCT CTTCCACGTG TCAAATACAT 
GCATCGCTTA GTGTCGATGA ACATAGTAGT AATAAGACTA GACTCTTTAC AGAACAAGAT 
GATGGGATGA ATTTGACCCA GTGTCATACA GCCTGTATTC AGACTTGGAT TCCTCCATCC 
AGGGAGGCCA CCTTGGGAGA GGTTAAAGGT GATAATACAA TTTATGGCAA TGAGTGTATG 
GAGTTGACAA CTAACTACAC GATACAGGTG TTGTCATCAG AAAATAACTT ACCTGAAAGA 
GAAAATCAAG CTCAAAATGA TGTGATTGAT GTTACAACAG TCGATGGAGC TACAGCTCCA 
GAGAAAAAAA CAGCCCTTAA GGATAAACTA AATGCTGCTT TTCACAGCTC TTTCCCAAAC 
CCTGAAAATA AACTTCATAT TACCAAATGT CATCCTATAG AGTCAGAAAC TCATACAGTC 
ACACAGATTT CTAATCAAAA TGCCAACACA TTGGCTATGA CCTCAGAATC TGTATGTTCT 
AGCCCAGCTA TTCAAGGGTA TAAAACAATT TTTTACTCTA GTAGCAATGA TGCAATGGAA 
CTGACTAAGT GTCTATCAAT TATGCAAGAG GAAAAAAAAT TGCTGAAGAC TGATGATAAG 
TATTCTAAAA TATGTACCAA TTCAGATGCT AGCCCACTCA GAGAGAAAAC TATTTATTCA 
GAAGAGGATA GCATGGACAT TACCAAAAGT CACACAGTTG CAATAGATAA TAAGATATTT 
AAACATGATC AAGAAAACAT TAAAAAAGAA ATAACAGCTC TACCAATATT TGAAAAAGAA 
ATGATGCTCC AAAATCTTAA AACTATGTCC AAAGATGAGA AAATGAATGT GAATTCTATT 
ACAGTTCCTC AAGTATCTAA AGAAAGTTTA CAACAGAGTC TGACAAATGC CTTATCTATT 
TCATTGGCTG ATAAAACAAT GGAATTCTTA GCAGGTGAAG ATATGGATTT GACTAAAAGT 
CACACAACTA AGTTAAGTCA AGTTATTCCT ACAACTTTTG ACTTAGCATC AAAGAATGTC 
ACCAAATCTT CCTCTCACAG CAAAAGCCCT TCAGATGAGT GGGAAGGCCT GGAAAAACAA 
ATTGTGCTAG GCCAGCATTC TAAACTTCCT TCACCACAGA GGAAAGATTT AGATGATCCT 
GACTGTTCTC ATCATAAGAT AATGTATTCA GAGGAGGTAC AAACTATGGA TCTAACCAAG 
AGCCACACTA TTGTGATTGG GTTTGGTTCT TCTGAGGTAC AAGAACACAG TAAGATTAAT 
TTAGAACACA AAAGCAGCCA ACTTACAGCA GAAAACAGAC AAACAGCTGT GAACATTCCT 
GGAATAAACT TTAGAGTAGT AACAACTAAT GATATGGACA TGTTAAAGGA CAGAAGTATA 
CATAAGCCAG AATTGCTGGA GGAAAAGCAA AACGTCAAAA TTTATGGAAG AAAAAGTATG 
GGTGGACTGA AGATTGATAA AACTATATTA TTTTCAGAGG GTAATGAGGA TGATATGGAT 
ATCACTAAGA GTTGCACAGT CAAAATAAAC CATCGGTCTT TATTAGATAA ACATGATTCT 
CATTTAGTAT CTTTGGCAGG AACTTCTAAA ACCATTTTGC ATGCACGTGG ACAGGATGAA 
ATGGAAATTA ATAGAAATCA CACGACTCCT TTAGAGTGTA AAATTATCTC ACCCAGTGAT 
ATAACTTCTG GTGATCTGAG TAAGACTGTG ATGTCTATAG ATGATCATGA GGAACTAGAA 
ATGACTAAGT CCCATACTGT TTTCATTGAC TACCAAGCAG AGGCAAAAGG TGTACTTCCA 
GACAGACTTG ACTTTCAATT ATCCAAAAAG AAAAGCCTAC AGAAGCCAAA AATGACATCT 
ACTCATGCTG AAGAGATTGT TTCCATTTCA AAAAATAGTG AAAGCAACTA TCTACCAGCC 
AAAGGCAGCC AGGTAACAAT ATTAGAGGAA GGATCTAATA GTGGGCCTGG GGAAGAAACT 
AATGATGCAC AAAAGCCTGG ATTTCTGAAT GAACCTCTGT CAGGCAAAAC TCAAAGAAGA 
GAAAGCCTTA AGCTCAAAAA TAAGACTATT ACATTTTCAG GAAATGATAA AAGTAATAGA 
GATATCACTC AGAGTTCTGT GGTAGAAATA AATAATGAAA CTAACCTGGA AGATAGAAAG 
GGCTTCAGTT TTGTGCCATT GGCAGGAACT TCCAAGCCAG TTTTATCTGC ATATCGGCCA 
GAGGACATGG AGATCTCTAT AAGTCAAACC TCTGCTTCGG AGTATAAAAC TATCCCACCA 
GATGAAATGA CTACTATACC TATGGATAAA ACTGTAATGT TTGTAGATAA TCTCAGTGAT 
CTGGGTGTCA CCAGATCCCA TACGTTTTTC ATCGATTGTC AAGCCAAAGA GAAAGTACTT 
CATGAATATA CCAACTTGGG AATACCAAAA CCACAAAACT TGAGTGGTTC TGAAGATAAT 
TCCTGTATTC AAGAAATCAC CAAAAAACCA GTAGCTGAAT ACAAACACCA TATGACAACT 
GTTATTCCTT CTAATACATT ATTTAGTGAT CAAAGTACAA TGAAAATCAA GATGCATAAA 
GCTGATATAG ATGAAGAGGG CATAGGAAAG GAAGTGGAGG CCGATACATT GAAACAAACT 
AAACTTGAAA GGTGTCTGTT AAATAGAACT GATGGAAAAA ATGTGGATTT TACAAGTAGT 
CATATAGCTG ATGTTTGTAC ATCCAGAGAC AAGTATTCCT CTTTACCAAA TGTTATTTCT 
TCTTCTGATA ATTTAGATAG TAATACTAAG TCCTTGTGTG ATAAAAGTAG GGAGAAAGCT 
TATAATTGCC AAGTGCCAAA TGAGTTTACT TTTGCAGATA ATTTACCAAG TAGATATCAC 
CTGGATTCTG AAATACCATC TGTCTTTGTA TCATGTCCTT CAAAAGAAGT TACTCAAACT 
GAAAGTGCCA TAGCACTACT TCAAGATCAA GATCAAGAGC CTCTGGGACT CTCAAAGTGT 
GCATCTCTTA AGTGTGCAAA GGATCAAATG GAGGCCTTTG TTGATGTTAG TGTTGCTTCT 
CAGCCTCATC TCTCAGCCCA ACAACCTCCA TCAACTCAAA AAGGGCAGGA TGCCGCCAAT 
AGAGATGAAG GAATGCTATC TAAAGCTGGG AAGAAGACTT TACCTTTTCT TATAGGAAAT 
GTCTCTGCAC CCACATGTGA AAATGCGTCC AAAATGCCTA CTAATGAGGA ACACTTTGCT 
GTAACCTATA ACAAAGAACT GGAAAAAAGT ATTCAAACAG ATAAATGTAA TACAAATGTA 
CACAACCATA GTAACTCAGT CCTGACAACA CAAGTCATTC AAACACATGC CAGTGCCGAA 
GCAGCATCAG ACTCTATAGT TCCATCTAAT GGTTCCTGTT TTAGTAGTGC CAAACCAAGT 
CTGAGTAATT TGAATAGAAA AACTGGGAAG GTTTTAGACT TTAATACTGT TCATATATTA 
CCTCCTGCAG AACAGTTACT TGAAGCAGGA AATCAGGCAC ATAGTATGAG TATAGTACAA 
GGTACAGAAA TACATAGCCT AGGTTCCAGC AATGACAGAG ATAAAGAAAG TAAAACTCTG 
CATAATGAGG CCGAAGCTAC ATCTGTACCA CTAAAAGCAG TTGTTAAAGA CAAAGCAAGA 
AGATGTTCAT TGGGAATATT TTTACCCAAA TTACCAAACA AGAAAAGTTG TAGTATCACT 
GGTGTTGATG ATGTGGAACA GATTCTAGCA GATGCAACTG ACTTAACTCA CCTAGAAACT 
CAGCCAGTCT GTAGTCAAGA TCCGGGCATT GGCTCTGTTG CAGCCAAACT GAACTTGAGT 
CCATCTCAGT ATATAAATGA GGAGAATCTC CCTGTATATC CCGGTGAGAT ACTTTCATCA 
GATTCTGTCA TCCTGGACAT TGAGGAAAGT GCTCCGATCG ACACATATCA TAAAGAGATT 
TTACCATCTG AAAACAAAAC AGAAACCTGC AGGGCCCAAA AAAGAGCCTG GGTTCAAGAA 
AATGAGGTTA AAAATGAGAA AAAAATCAGA ACACATGATG GTGCACAAGA TCAAGAGATT 
TTTGATCATT ATACTGAAGA GGACATCAAT AAAAGTATTA ACAATGTATT TCTAAAAAGC 
CTGAGCAGGA CACCGTCTAG TTCTAGCAGC TCTCTGGATT CAATCAAGGC TGATGGAATC 
TCTCTGGATG CCAGCACACA GCGTAATAGC CAAATGGAAT CACAGTTTCT TGGTGACACT 
ATCTGTGAAG AGAGTTTGAG GGAGAAACTG AAGGATGGAC AAATTACAAT CAAAGAGTTC 
TTCATACTTC TTCAAGTTCA TATCTTAATA CAGAAACCTA GGCAGAGCAC TCTCCCAGCC 
AAATTTACTA TAAACACATT ACCTACCACA GAAGATCTGA TGTTGAGACA ATATGTTTAC 
GGACCCAAGA TACAAATTTA CAGAGAAGAT TGTGAGCTTC TTCGACAGAA GATTGAAGAA 
TTGAAGGTAT CTGCGTTGAA CCAAGATAAA CTATTGGTGG ATGTAAATAG GAACTTGTGG 
GAAAAAATGA AAGACTACTC TGACGAAGAG CTGAAGACCT ATGGAATTTA CCTTAACAAA 
ATAAAATCAC GTTATACCAA GATGACGAAA GTCTTCACTC ACCAAGGAAA AGTGGATCTG 
TATAGCAAAT TGGTGCACTC AGCTGAGACT GAGAAGAGGA AACTTCAGAT AAAAATCAAC 
GAGATGGACA CCATACTTAA GAAGATCAAT AACTGTCTCA CTGCAGTGGA AATAGAAACT 
AAGAATTTGG AGGCTGAAGA GAAAGATGAT GCCATGGAAG ATTGGGATTC TGAAATACGA 
GATGCAGAGA AAGAACTGGA AAGGCTGAAA ACTGAGGAAG AGGAGCTTCA AAGAAAGTTC 
TTAGAAGTAG AGACACAGAA AACGCAGACC CTTGCTCAGA TAGAATTTAT AAAAGAACAA 
ACAACTGAAA CAGAAGAATT ATTGGATCAA TTGAGTTTTT CTGAGTGGGA TATCATCGAG 
TGGAGTGATG AACAAGCGGT ATTTACCTTT GTTTATGATT CAATAGAACT TATCATCACT 
TTTGGAGAGC CTCAGGTTGG TCTCCCTTTC TTGGACAAGG CTTACAGGAC GATTAATGAA 
CTCAGTTTTC AGTCTCTGTT AGACGAAGAT AAAGCGCCTC CTTCCTCCCT CTTGGTCCAC 
AAGCTTATTT TCCAGTACAT TGAAGAGCAG GAGTTCTGGA AGAAGAAGTG CACAACACAG 
CATCAGGTGC CCCAGATGCT TCAAGAACTC TCATTGGTAG TTAACCATTG TAGGCTTCTT 
GGAGAAGAGA TTGAATTTTT AAAGCGATGG GGACCAAATT ATAGCCTAAT GCATATAAAT 
GTGAATAATA CTGAATTACG GCTTCTATTC TCCAGTTCTG CAGCATTTGC AAAGTTTGAG 
ATAACTTTGT TTCCCTCGGC CCATTATCCC TTGGTCCCAC TACCATTCAC CATTCAAAAT 
CACCTTGGGA AGACTGGCCA TGATGAAATT GCTGCCGTTA TCTCTAAAGT GCCCTTAGAG 
GAAAACTACC TGAAGAATGT AGTCAAGCAG ATTTACCAAG ATCTACTTAA GGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008760321.1
CDS	100..6516
Translation	MDGIYSEANEENDNTQRPVRRQHSSILKPPRSPLQDLKCGNEIVQESNAPRTRKSSRRVSFADTIKVFQTESHMKTEKNSEISETEARKNVLTLQNKNLDDNYCAITGMNTLLCAPIQTQMQQKEFSITDCNPERKHANDQTVIFSDENQMDLTASHTVMIAKGLVDCTKHENSTKIDTTSFLENLKHRAANSRVKNDLACPIISINQNIFSEKKNSDNFIKRLKMGKYNTSSTELDKENAEIPVHSKDSNSASSTCQIHASLSVDEHSSNKTRLFTEQDDGMNLTQCHTACIQTWIPPSREATLGEVKGDNTIYGNECMELTTNYTIQVLSSENNLPERENQAQNDVIDVTTVDGATAPEKKTALKDKLNAAFHSSFPNPENKLHITKCHPIESETHTVTQISNQNANTLAMTSESVCSSPAIQGYKTIFYSSSNDAMELTKCLSIMQEEKKLLKTDDKYSKICTNSDASPLREKTIYSEEDSMDITKSHTVAIDNKIFKHDQENIKKEITALPIFEKEMMLQNLKTMSKDEKMNVNSITVPQVSKESLQQSLTNALSISLADKTMEFLAGEDMDLTKSHTTKLSQVIPTTFDLASKNVTKSSSHSKSPSDEWEGLEKQIVLGQHSKLPSPQRKDLDDPDCSHHKIMYSEEVQTMDLTKSHTIVIGFGSSEVQEHSKINLEHKSSQLTAENRQTAVNIPGINFRVVTTNDMDMLKDRSIHKPELLEEKQNVKIYGRKSMGGLKIDKTILFSEGNEDDMDITKSCTVKINHRSLLDKHDSHLVSLAGTSKTILHARGQDEMEINRNHTTPLECKIISPSDITSGDLSKTVMSIDDHEELEMTKSHTVFIDYQAEAKGVLPDRLDFQLSKKKSLQKPKMTSTHAEEIVSISKNSESNYLPAKGSQVTILEEGSNSGPGEETNDAQKPGFLNEPLSGKTQRRESLKLKNKTITFSGNDKSNRDITQSSVVEINNETNLEDRKGFSFVPLAGTSKPVLSAYRPEDMEISISQTSASEYKTIPPDEMTTIPMDKTVMFVDNLSDLGVTRSHTFFIDCQAKEKVLHEYTNLGIPKPQNLSGSEDNSCIQEITKKPVAEYKHHMTTVIPSNTLFSDQSTMKIKMHKADIDEEGIGKEVEADTLKQTKLERCLLNRTDGKNVDFTSSHIADVCTSRDKYSSLPNVISSSDNLDSNTKSLCDKSREKAYNCQVPNEFTFADNLPSRYHLDSEIPSVFVSCPSKEVTQTESAIALLQDQDQEPLGLSKCASLKCAKDQMEAFVDVSVASQPHLSAQQPPSTQKGQDAANRDEGMLSKAGKKTLPFLIGNVSAPTCENASKMPTNEEHFAVTYNKELEKSIQTDKCNTNVHNHSNSVLTTQVIQTHASAEAASDSIVPSNGSCFSSAKPSLSNLNRKTGKVLDFNTVHILPPAEQLLEAGNQAHSMSIVQGTEIHSLGSSNDRDKESKTLHNEAEATSVPLKAVVKDKARRCSLGIFLPKLPNKKSCSITGVDDVEQILADATDLTHLETQPVCSQDPGIGSVAAKLNLSPSQYINEENLPVYPGEILSSDSVILDIEESAPIDTYHKEILPSENKTETCRAQKRAWVQENEVKNEKKIRTHDGAQDQEIFDHYTEEDINKSINNVFLKSLSRTPSSSSSSLDSIKADGISLDASTQRNSQMESQFLGDTICEESLREKLKDGQITIKEFFILLQVHILIQKPRQSTLPAKFTINTLPTTEDLMLRQYVYGPKIQIYREDCELLRQKIEELKVSALNQDKLLVDVNRNLWEKMKDYSDEELKTYGIYLNKIKSRYTKMTKVFTHQGKVDLYSKLVHSAETEKRKLQIKINEMDTILKKINNCLTAVEIETKNLEAEEKDDAMEDWDSEIRDAEKELERLKTEEEELQRKFLEVETQKTQTLAQIEFIKEQTTETEELLDQLSFSEWDIIEWSDEQAVFTFVYDSIELIITFGEPQVGLPFLDKAYRTINELSFQSLLDEDKAPPSSLLVHKLIFQYIEEQEFWKKKCTTQHQVPQMLQELSLVVNHCRLLGEEIEFLKRWGPNYSLMHINVNNTELRLLFSSSAAFAKFEITLFPSAHYPLVPLPFTIQNHLGKTGHDEIAAVISKVPLEENYLKNVVKQIYQDLLKD

Target ORF information:

RefSeq Version	XM_008762099.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus kinetochore scaffold 1 (Knl1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008762099.2

ORF Insert Sequence:

ATGGATGGGA TATATTCAGA GGCTAATGAA GAGAATGACA ATACACAGAG GCCTGTTAGA 
AGACAGCATT CCTCAATTTT GAAACCCCCA AGGAGTCCTC TTCAGGACCT CAAATGTGGA 
AATGAAATAG TTCAGGAATC TAATGCTCCG AGAACCAGGA AAAGCTCTCG TCGGGTCAGC 
TTTGCAGATA CTATAAAAGT ATTCCAGACA GAGTCCCATA TGAAAACGGA AAAAAATTCA 
GAAATTTCAG AGACAGAAGC CAGAAAAAAT GTGTTGACCC TACAGAACAA GAATTTAGAT 
GATAACTATT GTGCAATTAC TGGAATGAAC ACTTTGCTTT GTGCTCCAAT TCAGACTCAG 
ATGCAACAAA AGGAGTTTTC AATTACAGAC TGCAACCCTG AAAGGAAACA TGCAAATGAT 
CAAACAGTCA TCTTTTCAGA TGAAAATCAG ATGGATCTGA CAGCAAGTCA CACGGTGATG 
ATTGCCAAAG GTCTTGTAGA CTGTACCAAA CATGAAAATT CCACAAAAAT AGATACCACA 
TCCTTTCTGG AGAATTTAAA GCATCGTGCT GCGAATTCAA GAGTTAAAAA CGATTTAGCA 
TGTCCAATAA TTTCTATAAA TCAAAACATT TTTTCAGAAA AGAAAAATTC TGACAACTTC 
ATAAAAAGAT TGAAAATGGG GAAATATAAT ACTTCTTCTA CAGAACTTGA TAAGGAAAAT 
GCTGAAATAC CTGTTCATTC CAAAGACTCA AACAGTGCCT CTTCCACGTG TCAAATACAT 
GCATCGCTTA GTGTCGATGA ACATAGTAGT AATAAGACTA GACTCTTTAC AGAACAAGAT 
GATGGGATGA ATTTGACCCA GTGTCATACA GCCTGTATTC AGACTTGGAT TCCTCCATCC 
AGGGAGGCCA CCTTGGGAGA GGTTAAAGGT GATAATACAA TTTATGGCAA TGAGTGTATG 
GAGTTGACAA CTAACTACAC GATACAGGTG TTGTCATCAG AAAATAACTT ACCTGAAAGA 
GAAAATCAAG CTCAAAATGA TGTGATTGAT GTTACAACAG TCGATGGAGC TACAGCTCCA 
GAGAAAAAAA CAGCCCTTAA GGATAAACTA AATGCTGCTT TTCACAGCTC TTTCCCAAAC 
CCTGAAAATA AACTTCATAT TACCAAATGT CATCCTATAG AGTCAGAAAC TCATACAGTC 
ACACAGATTT CTAATCAAAA TGCCAACACA TTGGCTATGA CCTCAGAATC TGTATGTTCT 
AGCCCAGCTA TTCAAGGGTA TAAAACAATT TTTTACTCTA GTAGCAATGA TGCAATGGAA 
CTGACTAAGT GTCTATCAAT TATGCAAGAG GAAAAAAAAT TGCTGAAGAC TGATGATAAG 
TATTCTAAAA TATGTACCAA TTCAGATGCT AGCCCACTCA GAGAGAAAAC TATTTATTCA 
GAAGAGGATA GCATGGACAT TACCAAAAGT CACACAGTTG CAATAGATAA TAAGATATTT 
AAACATGATC AAGAAAACAT TAAAAAAGAA ATAACAGCTC TACCAATATT TGAAAAAGAA 
ATGATGCTCC AAAATCTTAA AACTATGTCC AAAGATGAGA AAATGAATGT GAATTCTATT 
ACAGTTCCTC AAGTATCTAA AGAAAGTTTA CAACAGAGTC TGACAAATGC CTTATCTATT 
TCATTGGCTG ATAAAACAAT GGAATTCTTA GCAGGTGAAG ATATGGATTT GACTAAAAGT 
CACACAACTA AGTTAAGTCA AGTTATTCCT ACAACTTTTG ACTTAGCATC AAAGAATGTC 
ACCAAATCTT CCTCTCACAG CAAAAGCCCT TCAGATGAGT GGGAAGGCCT GGAAAAACAA 
ATTGTGCTAG GCCAGCATTC TAAACTTCCT TCACCACAGA GGAAAGATTT AGATGATCCT 
GACTGTTCTC ATCATAAGAT AATGTATTCA GAGGAGGTAC AAACTATGGA TCTAACCAAG 
AGCCACACTA TTGTGATTGG GTTTGGTTCT TCTGAGGTAC AAGAACACAG TAAGATTAAT 
TTAGAACACA AAAGCAGCCA ACTTACAGCA GAAAACAGAC AAACAGCTGT GAACATTCCT 
GGAATAAACT TTAGAGTAGT AACAACTAAT GATATGGACA TGTTAAAGGA CAGAAGTATA 
CATAAGCCAG AATTGCTGGA GGAAAAGCAA AACGTCAAAA TTTATGGAAG AAAAAGTATG 
GGTGGACTGA AGATTGATAA AACTATATTA TTTTCAGAGG GTAATGAGGA TGATATGGAT 
ATCACTAAGA GTTGCACAGT CAAAATAAAC CATCGGTCTT TATTAGATAA ACATGATTCT 
CATTTAGTAT CTTTGGCAGG AACTTCTAAA ACCATTTTGC ATGCACGTGG ACAGGATGAA 
ATGGAAATTA ATAGAAATCA CACGACTCCT TTAGAGTGTA AAATTATCTC ACCCAGTGAT 
ATAACTTCTG GTGATCTGAG TAAGACTGTG ATGTCTATAG ATGATCATGA GGAACTAGAA 
ATGACTAAGT CCCATACTGT TTTCATTGAC TACCAAGCAG AGGCAAAAGG TGTACTTCCA 
GACAGACTTG ACTTTCAATT ATCCAAAAAG AAAAGCCTAC AGAAGCCAAA AATGACATCT 
ACTCATGCTG AAGAGATTGT TTCCATTTCA AAAAATAGTG AAAGCAACTA TCTACCAGCC 
AAAGGCAGCC AGGTAACAAT ATTAGAGGAA GGATCTAATA GTGGGCCTGG GGAAGAAACT 
AATGATGCAC AAAAGCCTGG ATTTCTGAAT GAACCTCTGT CAGGCAAAAC TCAAAGAAGA 
GAAAGCCTTA AGCTCAAAAA TAAGACTATT ACATTTTCAG GAAATGATAA AAGTAATAGA 
GATATCACTC AGAGTTCTGT GGTAGAAATA AATAATGAAA CTAACCTGGA AGATAGAAAG 
GGCTTCAGTT TTGTGCCATT GGCAGGAACT TCCAAGCCAG TTTTATCTGC ATATCGGCCA 
GAGGACATGG AGATCTCTAT AAGTCAAACC TCTGCTTCGG AGTATAAAAC TATCCCACCA 
GATGAAATGA CTACTATACC TATGGATAAA ACTGTAATGT TTGTAGATAA TCTCAGTGAT 
CTGGGTGTCA CCAGATCCCA TACGTTTTTC ATCGATTGTC AAGCCAAAGA GAAAGTACTT 
CATGAATATA CCAACTTGGG AATACCAAAA CCACAAAACT TGAGTGGTTC TGAAGATAAT 
TCCTGTATTC AAGAAATCAC CAAAAAACCA GTAGCTGAAT ACAAACACCA TATGACAACT 
GTTATTCCTT CTAATACATT ATTTAGTGAT CAAAGTACAA TGAAAATCAA GATGCATAAA 
GCTGATATAG ATGAAGAGGG CATAGGAAAG GAAGTGGAGG CCGATACATT GAAACAAACT 
AAACTTGAAA GGTGTCTGTT AAATAGAACT GATGGAAAAA ATGTGGATTT TACAAGTAGT 
CATATAGCTG ATGTTTGTAC ATCCAGAGAC AAGTATTCCT CTTTACCAAA TGTTATTTCT 
TCTTCTGATA ATTTAGATAG TAATACTAAG TCCTTGTGTG ATAAAAGTAG GGAGAAAGCT 
TATAATTGCC AAGTGCCAAA TGAGTTTACT TTTGCAGATA ATTTACCAAG TAGATATCAC 
CTGGATTCTG AAATACCATC TGTCTTTGTA TCATGTCCTT CAAAAGAAGT TACTCAAACT 
GAAAGTGCCA TAGCACTACT TCAAGATCAA GATCAAGAGC CTCTGGGACT CTCAAAGTGT 
GCATCTCTTA AGTGTGCAAA GGATCAAATG GAGGCCTTTG TTGATGTTAG TGTTGCTTCT 
CAGCCTCATC TCTCAGCCCA ACAACCTCCA TCAACTCAAA AAGGGCAGGA TGCCGCCAAT 
AGAGATGAAG GAATGCTATC TAAAGCTGGG AAGAAGACTT TACCTTTTCT TATAGGAAAT 
GTCTCTGCAC CCACATGTGA AAATGCGTCC AAAATGCCTA CTAATGAGGA ACACTTTGCT 
GTAACCTATA ACAAAGAACT GGAAAAAAGT ATTCAAACAG ATAAATGTAA TACAAATGTA 
CACAACCATA GTAACTCAGT CCTGACAACA CAAGTCATTC AAACACATGC CAGTGCCGAA 
GCAGCATCAG ACTCTATAGT TCCATCTAAT GGTTCCTGTT TTAGTAGTGC CAAACCAAGT 
CTGAGTAATT TGAATAGAAA AACTGGGAAG GTTTTAGACT TTAATACTGT TCATATATTA 
CCTCCTGCAG AACAGTTACT TGAAGCAGGA AATCAGGCAC ATAGTATGAG TATAGTACAA 
GGTACAGAAA TACATAGCCT AGGTTCCAGC AATGACAGAG ATAAAGAAAG TAAAACTCTG 
CATAATGAGG CCGAAGCTAC ATCTGTACCA CTAAAAGCAG TTGTTAAAGA CAAAGCAAGA 
AGATGTTCAT TGGGAATATT TTTACCCAAA TTACCAAACA AGAAAAGTTG TAGTATCACT 
GGTGTTGATG ATGTGGAACA GATTCTAGCA GATGCAACTG ACTTAACTCA CCTAGAAACT 
CAGCCAGTCT GTAGTCAAGA TCCGGGCATT GGCTCTGTTG CAGCCAAACT GAACTTGAGT 
CCATCTCAGT ATATAAATGA GGAGAATCTC CCTGTATATC CCGGTGAGAT ACTTTCATCA 
GATTCTGTCA TCCTGGACAT TGAGGAAAGT GCTCCGATCG ACACATATCA TAAAGAGATT 
TTACCATCTG AAAACAAAAC AGAAACCTGC AGGGCCCAAA AAAGAGCCTG GGTTCAAGAA 
AATGAGGTTA AAAATGAGAA AAAAATCAGA ACACATGATG GTGCACAAGA TCAAGAGATT 
TTTGATCATT ATACTGAAGA GGACATCAAT AAAAGTATTA ACAATGTATT TCTAAAAAGC 
CTGAGCAGGA CACCGTCTAG TTCTAGCAGC TCTCTGGATT CAATCAAGGC TGATGGAATC 
TCTCTGGATG CCAGCACACA GCGTAATAGC CAAATGGAAT CACAGTTTCT TGGTGACACT 
ATCTGTGAAG AGAGTTTGAG GGAGAAACTG AAGGATGGAC AAATTACAAT CAAAGAGTTC 
TTCATACTTC TTCAAGTTCA TATCTTAATA CAGAAACCTA GGCAGAGCAC TCTCCCAGCC 
AAATTTACTA TAAACACATT ACCTACCACA GAAGATCTGA TGTTGAGACA ATATGTTTAC 
GGACCCAAGA TACAAATTTA CAGAGAAGAT TGTGAGCTTC TTCGACAGAA GATTGAAGAA 
TTGAAGGTAT CTGCGTTGAA CCAAGATAAA CTATTGGTGG ATGTAAATAG GAACTTGTGG 
GAAAAAATGA AAGACTACTC TGACGAAGAG CTGAAGACCT ATGGAATTTA CCTTAACAAA 
ATAAAATCAC GTTATACCAA GATGACGAAA GTCTTCACTC ACCAAGGAAA AGTGGATCTG 
TATAGCAAAT TGGTGCACTC AGCTGAGACT GAGAAGAGGA AACTTCAGAT AAAAATCAAC 
GAGATGGACA CCATACTTAA GAAGATCAAT AACTGTCTCA CTGCAGTGGA AATAGAAACT 
AAGAATTTGG AGGCTGAAGA GAAAGATGAT GCCATGGAAG ATTGGGATTC TGAAATACGA 
GATGCAGAGA AAGAACTGGA AAGGCTGAAA ACTGAGGAAG AGGAGCTTCA AAGAAAGTTC 
TTAGAAGTAG AGACACAGAA AACGCAGACC CTTGCTCAGA TAGAATTTAT AAAAGAACAA 
ACAACTGAAA CAGAAGAATT ATTGGATCAA TTGAGTTTTT CTGAGTGGGA TATCATCGAG 
TGGAGTGATG AACAAGCGGT ATTTACCTTT GTTTATGATT CAATAGAACT TATCATCACT 
TTTGGAGAGC CTCAGGTTGG TCTCCCTTTC TTGGACAAGG CTTACAGGAC GATTAATGAA 
CTCAGTTTTC AGTCTCTGTT AGACGAAGAT AAAGCGCCTC CTTCCTCCCT CTTGGTCCAC 
AAGCTTATTT TCCAGTACAT TGAAGAGCAG GAGTTCTGGA AGAAGAAGTG CACAACACAG 
CATCAGGTGC CCCAGATGCT TCAAGAACTC TCATTGGTAG TTAACCATTG TAGGCTTCTT 
GGAGAAGAGA TTGAATTTTT AAAGCGATGG GGACCAAATT ATAGCCTAAT GCATATAAAT 
GTGAATAATA CTGAATTACG GCTTCTATTC TCCAGTTCTG CAGCATTTGC AAAGTTTGAG 
ATAACTTTGT TTCCCTCGGC CCATTATCCC TTGGTCCCAC TACCATTCAC CATTCAAAAT 
CACCTTGGGA AGACTGGCCA TGATGAAATT GCTGCCGTTA TCTCTAAAGT GCCCTTAGAG 
GAAAACTACC TGAAGAATGT AGTCAAGCAG ATTTACCAAG ATCTACTTAA GGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Knl1 ( XM_008762097.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008760319.1 Rattus norvegicus kinetochore scaffold 1 (Knl1), transcript variant X3, mRNA.

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CloneID	ORa46967
Clone ID Related Accession (Same CDS sequence)	XM_008762099.2 , XM_008762097.2 , XM_008762096.1
Accession Version	XM_008762097.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6417bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	protein CASC5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008762097.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGGGA TATATTCAGA GGCTAATGAA GAGAATGACA ATACACAGAG GCCTGTTAGA 
AGACAGCATT CCTCAATTTT GAAACCCCCA AGGAGTCCTC TTCAGGACCT CAAATGTGGA 
AATGAAATAG TTCAGGAATC TAATGCTCCG AGAACCAGGA AAAGCTCTCG TCGGGTCAGC 
TTTGCAGATA CTATAAAAGT ATTCCAGACA GAGTCCCATA TGAAAACGGA AAAAAATTCA 
GAAATTTCAG AGACAGAAGC CAGAAAAAAT GTGTTGACCC TACAGAACAA GAATTTAGAT 
GATAACTATT GTGCAATTAC TGGAATGAAC ACTTTGCTTT GTGCTCCAAT TCAGACTCAG 
ATGCAACAAA AGGAGTTTTC AATTACAGAC TGCAACCCTG AAAGGAAACA TGCAAATGAT 
CAAACAGTCA TCTTTTCAGA TGAAAATCAG ATGGATCTGA CAGCAAGTCA CACGGTGATG 
ATTGCCAAAG GTCTTGTAGA CTGTACCAAA CATGAAAATT CCACAAAAAT AGATACCACA 
TCCTTTCTGG AGAATTTAAA GCATCGTGCT GCGAATTCAA GAGTTAAAAA CGATTTAGCA 
TGTCCAATAA TTTCTATAAA TCAAAACATT TTTTCAGAAA AGAAAAATTC TGACAACTTC 
ATAAAAAGAT TGAAAATGGG GAAATATAAT ACTTCTTCTA CAGAACTTGA TAAGGAAAAT 
GCTGAAATAC CTGTTCATTC CAAAGACTCA AACAGTGCCT CTTCCACGTG TCAAATACAT 
GCATCGCTTA GTGTCGATGA ACATAGTAGT AATAAGACTA GACTCTTTAC AGAACAAGAT 
GATGGGATGA ATTTGACCCA GTGTCATACA GCCTGTATTC AGACTTGGAT TCCTCCATCC 
AGGGAGGCCA CCTTGGGAGA GGTTAAAGGT GATAATACAA TTTATGGCAA TGAGTGTATG 
GAGTTGACAA CTAACTACAC GATACAGGTG TTGTCATCAG AAAATAACTT ACCTGAAAGA 
GAAAATCAAG CTCAAAATGA TGTGATTGAT GTTACAACAG TCGATGGAGC TACAGCTCCA 
GAGAAAAAAA CAGCCCTTAA GGATAAACTA AATGCTGCTT TTCACAGCTC TTTCCCAAAC 
CCTGAAAATA AACTTCATAT TACCAAATGT CATCCTATAG AGTCAGAAAC TCATACAGTC 
ACACAGATTT CTAATCAAAA TGCCAACACA TTGGCTATGA CCTCAGAATC TGTATGTTCT 
AGCCCAGCTA TTCAAGGGTA TAAAACAATT TTTTACTCTA GTAGCAATGA TGCAATGGAA 
CTGACTAAGT GTCTATCAAT TATGCAAGAG GAAAAAAAAT TGCTGAAGAC TGATGATAAG 
TATTCTAAAA TATGTACCAA TTCAGATGCT AGCCCACTCA GAGAGAAAAC TATTTATTCA 
GAAGAGGATA GCATGGACAT TACCAAAAGT CACACAGTTG CAATAGATAA TAAGATATTT 
AAACATGATC AAGAAAACAT TAAAAAAGAA ATAACAGCTC TACCAATATT TGAAAAAGAA 
ATGATGCTCC AAAATCTTAA AACTATGTCC AAAGATGAGA AAATGAATGT GAATTCTATT 
ACAGTTCCTC AAGTATCTAA AGAAAGTTTA CAACAGAGTC TGACAAATGC CTTATCTATT 
TCATTGGCTG ATAAAACAAT GGAATTCTTA GCAGGTGAAG ATATGGATTT GACTAAAAGT 
CACACAACTA AGTTAAGTCA AGTTATTCCT ACAACTTTTG ACTTAGCATC AAAGAATGTC 
ACCAAATCTT CCTCTCACAG CAAAAGCCCT TCAGATGAGT GGGAAGGCCT GGAAAAACAA 
ATTGTGCTAG GCCAGCATTC TAAACTTCCT TCACCACAGA GGAAAGATTT AGATGATCCT 
GACTGTTCTC ATCATAAGAT AATGTATTCA GAGGAGGTAC AAACTATGGA TCTAACCAAG 
AGCCACACTA TTGTGATTGG GTTTGGTTCT TCTGAGGTAC AAGAACACAG TAAGATTAAT 
TTAGAACACA AAAGCAGCCA ACTTACAGCA GAAAACAGAC AAACAGCTGT GAACATTCCT 
GGAATAAACT TTAGAGTAGT AACAACTAAT GATATGGACA TGTTAAAGGA CAGAAGTATA 
CATAAGCCAG AATTGCTGGA GGAAAAGCAA AACGTCAAAA TTTATGGAAG AAAAAGTATG 
GGTGGACTGA AGATTGATAA AACTATATTA TTTTCAGAGG GTAATGAGGA TGATATGGAT 
ATCACTAAGA GTTGCACAGT CAAAATAAAC CATCGGTCTT TATTAGATAA ACATGATTCT 
CATTTAGTAT CTTTGGCAGG AACTTCTAAA ACCATTTTGC ATGCACGTGG ACAGGATGAA 
ATGGAAATTA ATAGAAATCA CACGACTCCT TTAGAGTGTA AAATTATCTC ACCCAGTGAT 
ATAACTTCTG GTGATCTGAG TAAGACTGTG ATGTCTATAG ATGATCATGA GGAACTAGAA 
ATGACTAAGT CCCATACTGT TTTCATTGAC TACCAAGCAG AGGCAAAAGG TGTACTTCCA 
GACAGACTTG ACTTTCAATT ATCCAAAAAG AAAAGCCTAC AGAAGCCAAA AATGACATCT 
ACTCATGCTG AAGAGATTGT TTCCATTTCA AAAAATAGTG AAAGCAACTA TCTACCAGCC 
AAAGGCAGCC AGGTAACAAT ATTAGAGGAA GGATCTAATA GTGGGCCTGG GGAAGAAACT 
AATGATGCAC AAAAGCCTGG ATTTCTGAAT GAACCTCTGT CAGGCAAAAC TCAAAGAAGA 
GAAAGCCTTA AGCTCAAAAA TAAGACTATT ACATTTTCAG GAAATGATAA AAGTAATAGA 
GATATCACTC AGAGTTCTGT GGTAGAAATA AATAATGAAA CTAACCTGGA AGATAGAAAG 
GGCTTCAGTT TTGTGCCATT GGCAGGAACT TCCAAGCCAG TTTTATCTGC ATATCGGCCA 
GAGGACATGG AGATCTCTAT AAGTCAAACC TCTGCTTCGG AGTATAAAAC TATCCCACCA 
GATGAAATGA CTACTATACC TATGGATAAA ACTGTAATGT TTGTAGATAA TCTCAGTGAT 
CTGGGTGTCA CCAGATCCCA TACGTTTTTC ATCGATTGTC AAGCCAAAGA GAAAGTACTT 
CATGAATATA CCAACTTGGG AATACCAAAA CCACAAAACT TGAGTGGTTC TGAAGATAAT 
TCCTGTATTC AAGAAATCAC CAAAAAACCA GTAGCTGAAT ACAAACACCA TATGACAACT 
GTTATTCCTT CTAATACATT ATTTAGTGAT CAAAGTACAA TGAAAATCAA GATGCATAAA 
GCTGATATAG ATGAAGAGGG CATAGGAAAG GAAGTGGAGG CCGATACATT GAAACAAACT 
AAACTTGAAA GGTGTCTGTT AAATAGAACT GATGGAAAAA ATGTGGATTT TACAAGTAGT 
CATATAGCTG ATGTTTGTAC ATCCAGAGAC AAGTATTCCT CTTTACCAAA TGTTATTTCT 
TCTTCTGATA ATTTAGATAG TAATACTAAG TCCTTGTGTG ATAAAAGTAG GGAGAAAGCT 
TATAATTGCC AAGTGCCAAA TGAGTTTACT TTTGCAGATA ATTTACCAAG TAGATATCAC 
CTGGATTCTG AAATACCATC TGTCTTTGTA TCATGTCCTT CAAAAGAAGT TACTCAAACT 
GAAAGTGCCA TAGCACTACT TCAAGATCAA GATCAAGAGC CTCTGGGACT CTCAAAGTGT 
GCATCTCTTA AGTGTGCAAA GGATCAAATG GAGGCCTTTG TTGATGTTAG TGTTGCTTCT 
CAGCCTCATC TCTCAGCCCA ACAACCTCCA TCAACTCAAA AAGGGCAGGA TGCCGCCAAT 
AGAGATGAAG GAATGCTATC TAAAGCTGGG AAGAAGACTT TACCTTTTCT TATAGGAAAT 
GTCTCTGCAC CCACATGTGA AAATGCGTCC AAAATGCCTA CTAATGAGGA ACACTTTGCT 
GTAACCTATA ACAAAGAACT GGAAAAAAGT ATTCAAACAG ATAAATGTAA TACAAATGTA 
CACAACCATA GTAACTCAGT CCTGACAACA CAAGTCATTC AAACACATGC CAGTGCCGAA 
GCAGCATCAG ACTCTATAGT TCCATCTAAT GGTTCCTGTT TTAGTAGTGC CAAACCAAGT 
CTGAGTAATT TGAATAGAAA AACTGGGAAG GTTTTAGACT TTAATACTGT TCATATATTA 
CCTCCTGCAG AACAGTTACT TGAAGCAGGA AATCAGGCAC ATAGTATGAG TATAGTACAA 
GGTACAGAAA TACATAGCCT AGGTTCCAGC AATGACAGAG ATAAAGAAAG TAAAACTCTG 
CATAATGAGG CCGAAGCTAC ATCTGTACCA CTAAAAGCAG TTGTTAAAGA CAAAGCAAGA 
AGATGTTCAT TGGGAATATT TTTACCCAAA TTACCAAACA AGAAAAGTTG TAGTATCACT 
GGTGTTGATG ATGTGGAACA GATTCTAGCA GATGCAACTG ACTTAACTCA CCTAGAAACT 
CAGCCAGTCT GTAGTCAAGA TCCGGGCATT GGCTCTGTTG CAGCCAAACT GAACTTGAGT 
CCATCTCAGT ATATAAATGA GGAGAATCTC CCTGTATATC CCGGTGAGAT ACTTTCATCA 
GATTCTGTCA TCCTGGACAT TGAGGAAAGT GCTCCGATCG ACACATATCA TAAAGAGATT 
TTACCATCTG AAAACAAAAC AGAAACCTGC AGGGCCCAAA AAAGAGCCTG GGTTCAAGAA 
AATGAGGTTA AAAATGAGAA AAAAATCAGA ACACATGATG GTGCACAAGA TCAAGAGATT 
TTTGATCATT ATACTGAAGA GGACATCAAT AAAAGTATTA ACAATGTATT TCTAAAAAGC 
CTGAGCAGGA CACCGTCTAG TTCTAGCAGC TCTCTGGATT CAATCAAGGC TGATGGAATC 
TCTCTGGATG CCAGCACACA GCGTAATAGC CAAATGGAAT CACAGTTTCT TGGTGACACT 
ATCTGTGAAG AGAGTTTGAG GGAGAAACTG AAGGATGGAC AAATTACAAT CAAAGAGTTC 
TTCATACTTC TTCAAGTTCA TATCTTAATA CAGAAACCTA GGCAGAGCAC TCTCCCAGCC 
AAATTTACTA TAAACACATT ACCTACCACA GAAGATCTGA TGTTGAGACA ATATGTTTAC 
GGACCCAAGA TACAAATTTA CAGAGAAGAT TGTGAGCTTC TTCGACAGAA GATTGAAGAA 
TTGAAGGTAT CTGCGTTGAA CCAAGATAAA CTATTGGTGG ATGTAAATAG GAACTTGTGG 
GAAAAAATGA AAGACTACTC TGACGAAGAG CTGAAGACCT ATGGAATTTA CCTTAACAAA 
ATAAAATCAC GTTATACCAA GATGACGAAA GTCTTCACTC ACCAAGGAAA AGTGGATCTG 
TATAGCAAAT TGGTGCACTC AGCTGAGACT GAGAAGAGGA AACTTCAGAT AAAAATCAAC 
GAGATGGACA CCATACTTAA GAAGATCAAT AACTGTCTCA CTGCAGTGGA AATAGAAACT 
AAGAATTTGG AGGCTGAAGA GAAAGATGAT GCCATGGAAG ATTGGGATTC TGAAATACGA 
GATGCAGAGA AAGAACTGGA AAGGCTGAAA ACTGAGGAAG AGGAGCTTCA AAGAAAGTTC 
TTAGAAGTAG AGACACAGAA AACGCAGACC CTTGCTCAGA TAGAATTTAT AAAAGAACAA 
ACAACTGAAA CAGAAGAATT ATTGGATCAA TTGAGTTTTT CTGAGTGGGA TATCATCGAG 
TGGAGTGATG AACAAGCGGT ATTTACCTTT GTTTATGATT CAATAGAACT TATCATCACT 
TTTGGAGAGC CTCAGGTTGG TCTCCCTTTC TTGGACAAGG CTTACAGGAC GATTAATGAA 
CTCAGTTTTC AGTCTCTGTT AGACGAAGAT AAAGCGCCTC CTTCCTCCCT CTTGGTCCAC 
AAGCTTATTT TCCAGTACAT TGAAGAGCAG GAGTTCTGGA AGAAGAAGTG CACAACACAG 
CATCAGGTGC CCCAGATGCT TCAAGAACTC TCATTGGTAG TTAACCATTG TAGGCTTCTT 
GGAGAAGAGA TTGAATTTTT AAAGCGATGG GGACCAAATT ATAGCCTAAT GCATATAAAT 
GTGAATAATA CTGAATTACG GCTTCTATTC TCCAGTTCTG CAGCATTTGC AAAGTTTGAG 
ATAACTTTGT TTCCCTCGGC CCATTATCCC TTGGTCCCAC TACCATTCAC CATTCAAAAT 
CACCTTGGGA AGACTGGCCA TGATGAAATT GCTGCCGTTA TCTCTAAAGT GCCCTTAGAG 
GAAAACTACC TGAAGAATGT AGTCAAGCAG ATTTACCAAG ATCTACTTAA GGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008760319.1
CDS	172..6588
Translation	MDGIYSEANEENDNTQRPVRRQHSSILKPPRSPLQDLKCGNEIVQESNAPRTRKSSRRVSFADTIKVFQTESHMKTEKNSEISETEARKNVLTLQNKNLDDNYCAITGMNTLLCAPIQTQMQQKEFSITDCNPERKHANDQTVIFSDENQMDLTASHTVMIAKGLVDCTKHENSTKIDTTSFLENLKHRAANSRVKNDLACPIISINQNIFSEKKNSDNFIKRLKMGKYNTSSTELDKENAEIPVHSKDSNSASSTCQIHASLSVDEHSSNKTRLFTEQDDGMNLTQCHTACIQTWIPPSREATLGEVKGDNTIYGNECMELTTNYTIQVLSSENNLPERENQAQNDVIDVTTVDGATAPEKKTALKDKLNAAFHSSFPNPENKLHITKCHPIESETHTVTQISNQNANTLAMTSESVCSSPAIQGYKTIFYSSSNDAMELTKCLSIMQEEKKLLKTDDKYSKICTNSDASPLREKTIYSEEDSMDITKSHTVAIDNKIFKHDQENIKKEITALPIFEKEMMLQNLKTMSKDEKMNVNSITVPQVSKESLQQSLTNALSISLADKTMEFLAGEDMDLTKSHTTKLSQVIPTTFDLASKNVTKSSSHSKSPSDEWEGLEKQIVLGQHSKLPSPQRKDLDDPDCSHHKIMYSEEVQTMDLTKSHTIVIGFGSSEVQEHSKINLEHKSSQLTAENRQTAVNIPGINFRVVTTNDMDMLKDRSIHKPELLEEKQNVKIYGRKSMGGLKIDKTILFSEGNEDDMDITKSCTVKINHRSLLDKHDSHLVSLAGTSKTILHARGQDEMEINRNHTTPLECKIISPSDITSGDLSKTVMSIDDHEELEMTKSHTVFIDYQAEAKGVLPDRLDFQLSKKKSLQKPKMTSTHAEEIVSISKNSESNYLPAKGSQVTILEEGSNSGPGEETNDAQKPGFLNEPLSGKTQRRESLKLKNKTITFSGNDKSNRDITQSSVVEINNETNLEDRKGFSFVPLAGTSKPVLSAYRPEDMEISISQTSASEYKTIPPDEMTTIPMDKTVMFVDNLSDLGVTRSHTFFIDCQAKEKVLHEYTNLGIPKPQNLSGSEDNSCIQEITKKPVAEYKHHMTTVIPSNTLFSDQSTMKIKMHKADIDEEGIGKEVEADTLKQTKLERCLLNRTDGKNVDFTSSHIADVCTSRDKYSSLPNVISSSDNLDSNTKSLCDKSREKAYNCQVPNEFTFADNLPSRYHLDSEIPSVFVSCPSKEVTQTESAIALLQDQDQEPLGLSKCASLKCAKDQMEAFVDVSVASQPHLSAQQPPSTQKGQDAANRDEGMLSKAGKKTLPFLIGNVSAPTCENASKMPTNEEHFAVTYNKELEKSIQTDKCNTNVHNHSNSVLTTQVIQTHASAEAASDSIVPSNGSCFSSAKPSLSNLNRKTGKVLDFNTVHILPPAEQLLEAGNQAHSMSIVQGTEIHSLGSSNDRDKESKTLHNEAEATSVPLKAVVKDKARRCSLGIFLPKLPNKKSCSITGVDDVEQILADATDLTHLETQPVCSQDPGIGSVAAKLNLSPSQYINEENLPVYPGEILSSDSVILDIEESAPIDTYHKEILPSENKTETCRAQKRAWVQENEVKNEKKIRTHDGAQDQEIFDHYTEEDINKSINNVFLKSLSRTPSSSSSSLDSIKADGISLDASTQRNSQMESQFLGDTICEESLREKLKDGQITIKEFFILLQVHILIQKPRQSTLPAKFTINTLPTTEDLMLRQYVYGPKIQIYREDCELLRQKIEELKVSALNQDKLLVDVNRNLWEKMKDYSDEELKTYGIYLNKIKSRYTKMTKVFTHQGKVDLYSKLVHSAETEKRKLQIKINEMDTILKKINNCLTAVEIETKNLEAEEKDDAMEDWDSEIRDAEKELERLKTEEEELQRKFLEVETQKTQTLAQIEFIKEQTTETEELLDQLSFSEWDIIEWSDEQAVFTFVYDSIELIITFGEPQVGLPFLDKAYRTINELSFQSLLDEDKAPPSSLLVHKLIFQYIEEQEFWKKKCTTQHQVPQMLQELSLVVNHCRLLGEEIEFLKRWGPNYSLMHINVNNTELRLLFSSSAAFAKFEITLFPSAHYPLVPLPFTIQNHLGKTGHDEIAAVISKVPLEENYLKNVVKQIYQDLLKD

Target ORF information:

RefSeq Version	XM_008762097.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus kinetochore scaffold 1 (Knl1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008762097.2

ORF Insert Sequence:

ATGGATGGGA TATATTCAGA GGCTAATGAA GAGAATGACA ATACACAGAG GCCTGTTAGA 
AGACAGCATT CCTCAATTTT GAAACCCCCA AGGAGTCCTC TTCAGGACCT CAAATGTGGA 
AATGAAATAG TTCAGGAATC TAATGCTCCG AGAACCAGGA AAAGCTCTCG TCGGGTCAGC 
TTTGCAGATA CTATAAAAGT ATTCCAGACA GAGTCCCATA TGAAAACGGA AAAAAATTCA 
GAAATTTCAG AGACAGAAGC CAGAAAAAAT GTGTTGACCC TACAGAACAA GAATTTAGAT 
GATAACTATT GTGCAATTAC TGGAATGAAC ACTTTGCTTT GTGCTCCAAT TCAGACTCAG 
ATGCAACAAA AGGAGTTTTC AATTACAGAC TGCAACCCTG AAAGGAAACA TGCAAATGAT 
CAAACAGTCA TCTTTTCAGA TGAAAATCAG ATGGATCTGA CAGCAAGTCA CACGGTGATG 
ATTGCCAAAG GTCTTGTAGA CTGTACCAAA CATGAAAATT CCACAAAAAT AGATACCACA 
TCCTTTCTGG AGAATTTAAA GCATCGTGCT GCGAATTCAA GAGTTAAAAA CGATTTAGCA 
TGTCCAATAA TTTCTATAAA TCAAAACATT TTTTCAGAAA AGAAAAATTC TGACAACTTC 
ATAAAAAGAT TGAAAATGGG GAAATATAAT ACTTCTTCTA CAGAACTTGA TAAGGAAAAT 
GCTGAAATAC CTGTTCATTC CAAAGACTCA AACAGTGCCT CTTCCACGTG TCAAATACAT 
GCATCGCTTA GTGTCGATGA ACATAGTAGT AATAAGACTA GACTCTTTAC AGAACAAGAT 
GATGGGATGA ATTTGACCCA GTGTCATACA GCCTGTATTC AGACTTGGAT TCCTCCATCC 
AGGGAGGCCA CCTTGGGAGA GGTTAAAGGT GATAATACAA TTTATGGCAA TGAGTGTATG 
GAGTTGACAA CTAACTACAC GATACAGGTG TTGTCATCAG AAAATAACTT ACCTGAAAGA 
GAAAATCAAG CTCAAAATGA TGTGATTGAT GTTACAACAG TCGATGGAGC TACAGCTCCA 
GAGAAAAAAA CAGCCCTTAA GGATAAACTA AATGCTGCTT TTCACAGCTC TTTCCCAAAC 
CCTGAAAATA AACTTCATAT TACCAAATGT CATCCTATAG AGTCAGAAAC TCATACAGTC 
ACACAGATTT CTAATCAAAA TGCCAACACA TTGGCTATGA CCTCAGAATC TGTATGTTCT 
AGCCCAGCTA TTCAAGGGTA TAAAACAATT TTTTACTCTA GTAGCAATGA TGCAATGGAA 
CTGACTAAGT GTCTATCAAT TATGCAAGAG GAAAAAAAAT TGCTGAAGAC TGATGATAAG 
TATTCTAAAA TATGTACCAA TTCAGATGCT AGCCCACTCA GAGAGAAAAC TATTTATTCA 
GAAGAGGATA GCATGGACAT TACCAAAAGT CACACAGTTG CAATAGATAA TAAGATATTT 
AAACATGATC AAGAAAACAT TAAAAAAGAA ATAACAGCTC TACCAATATT TGAAAAAGAA 
ATGATGCTCC AAAATCTTAA AACTATGTCC AAAGATGAGA AAATGAATGT GAATTCTATT 
ACAGTTCCTC AAGTATCTAA AGAAAGTTTA CAACAGAGTC TGACAAATGC CTTATCTATT 
TCATTGGCTG ATAAAACAAT GGAATTCTTA GCAGGTGAAG ATATGGATTT GACTAAAAGT 
CACACAACTA AGTTAAGTCA AGTTATTCCT ACAACTTTTG ACTTAGCATC AAAGAATGTC 
ACCAAATCTT CCTCTCACAG CAAAAGCCCT TCAGATGAGT GGGAAGGCCT GGAAAAACAA 
ATTGTGCTAG GCCAGCATTC TAAACTTCCT TCACCACAGA GGAAAGATTT AGATGATCCT 
GACTGTTCTC ATCATAAGAT AATGTATTCA GAGGAGGTAC AAACTATGGA TCTAACCAAG 
AGCCACACTA TTGTGATTGG GTTTGGTTCT TCTGAGGTAC AAGAACACAG TAAGATTAAT 
TTAGAACACA AAAGCAGCCA ACTTACAGCA GAAAACAGAC AAACAGCTGT GAACATTCCT 
GGAATAAACT TTAGAGTAGT AACAACTAAT GATATGGACA TGTTAAAGGA CAGAAGTATA 
CATAAGCCAG AATTGCTGGA GGAAAAGCAA AACGTCAAAA TTTATGGAAG AAAAAGTATG 
GGTGGACTGA AGATTGATAA AACTATATTA TTTTCAGAGG GTAATGAGGA TGATATGGAT 
ATCACTAAGA GTTGCACAGT CAAAATAAAC CATCGGTCTT TATTAGATAA ACATGATTCT 
CATTTAGTAT CTTTGGCAGG AACTTCTAAA ACCATTTTGC ATGCACGTGG ACAGGATGAA 
ATGGAAATTA ATAGAAATCA CACGACTCCT TTAGAGTGTA AAATTATCTC ACCCAGTGAT 
ATAACTTCTG GTGATCTGAG TAAGACTGTG ATGTCTATAG ATGATCATGA GGAACTAGAA 
ATGACTAAGT CCCATACTGT TTTCATTGAC TACCAAGCAG AGGCAAAAGG TGTACTTCCA 
GACAGACTTG ACTTTCAATT ATCCAAAAAG AAAAGCCTAC AGAAGCCAAA AATGACATCT 
ACTCATGCTG AAGAGATTGT TTCCATTTCA AAAAATAGTG AAAGCAACTA TCTACCAGCC 
AAAGGCAGCC AGGTAACAAT ATTAGAGGAA GGATCTAATA GTGGGCCTGG GGAAGAAACT 
AATGATGCAC AAAAGCCTGG ATTTCTGAAT GAACCTCTGT CAGGCAAAAC TCAAAGAAGA 
GAAAGCCTTA AGCTCAAAAA TAAGACTATT ACATTTTCAG GAAATGATAA AAGTAATAGA 
GATATCACTC AGAGTTCTGT GGTAGAAATA AATAATGAAA CTAACCTGGA AGATAGAAAG 
GGCTTCAGTT TTGTGCCATT GGCAGGAACT TCCAAGCCAG TTTTATCTGC ATATCGGCCA 
GAGGACATGG AGATCTCTAT AAGTCAAACC TCTGCTTCGG AGTATAAAAC TATCCCACCA 
GATGAAATGA CTACTATACC TATGGATAAA ACTGTAATGT TTGTAGATAA TCTCAGTGAT 
CTGGGTGTCA CCAGATCCCA TACGTTTTTC ATCGATTGTC AAGCCAAAGA GAAAGTACTT 
CATGAATATA CCAACTTGGG AATACCAAAA CCACAAAACT TGAGTGGTTC TGAAGATAAT 
TCCTGTATTC AAGAAATCAC CAAAAAACCA GTAGCTGAAT ACAAACACCA TATGACAACT 
GTTATTCCTT CTAATACATT ATTTAGTGAT CAAAGTACAA TGAAAATCAA GATGCATAAA 
GCTGATATAG ATGAAGAGGG CATAGGAAAG GAAGTGGAGG CCGATACATT GAAACAAACT 
AAACTTGAAA GGTGTCTGTT AAATAGAACT GATGGAAAAA ATGTGGATTT TACAAGTAGT 
CATATAGCTG ATGTTTGTAC ATCCAGAGAC AAGTATTCCT CTTTACCAAA TGTTATTTCT 
TCTTCTGATA ATTTAGATAG TAATACTAAG TCCTTGTGTG ATAAAAGTAG GGAGAAAGCT 
TATAATTGCC AAGTGCCAAA TGAGTTTACT TTTGCAGATA ATTTACCAAG TAGATATCAC 
CTGGATTCTG AAATACCATC TGTCTTTGTA TCATGTCCTT CAAAAGAAGT TACTCAAACT 
GAAAGTGCCA TAGCACTACT TCAAGATCAA GATCAAGAGC CTCTGGGACT CTCAAAGTGT 
GCATCTCTTA AGTGTGCAAA GGATCAAATG GAGGCCTTTG TTGATGTTAG TGTTGCTTCT 
CAGCCTCATC TCTCAGCCCA ACAACCTCCA TCAACTCAAA AAGGGCAGGA TGCCGCCAAT 
AGAGATGAAG GAATGCTATC TAAAGCTGGG AAGAAGACTT TACCTTTTCT TATAGGAAAT 
GTCTCTGCAC CCACATGTGA AAATGCGTCC AAAATGCCTA CTAATGAGGA ACACTTTGCT 
GTAACCTATA ACAAAGAACT GGAAAAAAGT ATTCAAACAG ATAAATGTAA TACAAATGTA 
CACAACCATA GTAACTCAGT CCTGACAACA CAAGTCATTC AAACACATGC CAGTGCCGAA 
GCAGCATCAG ACTCTATAGT TCCATCTAAT GGTTCCTGTT TTAGTAGTGC CAAACCAAGT 
CTGAGTAATT TGAATAGAAA AACTGGGAAG GTTTTAGACT TTAATACTGT TCATATATTA 
CCTCCTGCAG AACAGTTACT TGAAGCAGGA AATCAGGCAC ATAGTATGAG TATAGTACAA 
GGTACAGAAA TACATAGCCT AGGTTCCAGC AATGACAGAG ATAAAGAAAG TAAAACTCTG 
CATAATGAGG CCGAAGCTAC ATCTGTACCA CTAAAAGCAG TTGTTAAAGA CAAAGCAAGA 
AGATGTTCAT TGGGAATATT TTTACCCAAA TTACCAAACA AGAAAAGTTG TAGTATCACT 
GGTGTTGATG ATGTGGAACA GATTCTAGCA GATGCAACTG ACTTAACTCA CCTAGAAACT 
CAGCCAGTCT GTAGTCAAGA TCCGGGCATT GGCTCTGTTG CAGCCAAACT GAACTTGAGT 
CCATCTCAGT ATATAAATGA GGAGAATCTC CCTGTATATC CCGGTGAGAT ACTTTCATCA 
GATTCTGTCA TCCTGGACAT TGAGGAAAGT GCTCCGATCG ACACATATCA TAAAGAGATT 
TTACCATCTG AAAACAAAAC AGAAACCTGC AGGGCCCAAA AAAGAGCCTG GGTTCAAGAA 
AATGAGGTTA AAAATGAGAA AAAAATCAGA ACACATGATG GTGCACAAGA TCAAGAGATT 
TTTGATCATT ATACTGAAGA GGACATCAAT AAAAGTATTA ACAATGTATT TCTAAAAAGC 
CTGAGCAGGA CACCGTCTAG TTCTAGCAGC TCTCTGGATT CAATCAAGGC TGATGGAATC 
TCTCTGGATG CCAGCACACA GCGTAATAGC CAAATGGAAT CACAGTTTCT TGGTGACACT 
ATCTGTGAAG AGAGTTTGAG GGAGAAACTG AAGGATGGAC AAATTACAAT CAAAGAGTTC 
TTCATACTTC TTCAAGTTCA TATCTTAATA CAGAAACCTA GGCAGAGCAC TCTCCCAGCC 
AAATTTACTA TAAACACATT ACCTACCACA GAAGATCTGA TGTTGAGACA ATATGTTTAC 
GGACCCAAGA TACAAATTTA CAGAGAAGAT TGTGAGCTTC TTCGACAGAA GATTGAAGAA 
TTGAAGGTAT CTGCGTTGAA CCAAGATAAA CTATTGGTGG ATGTAAATAG GAACTTGTGG 
GAAAAAATGA AAGACTACTC TGACGAAGAG CTGAAGACCT ATGGAATTTA CCTTAACAAA 
ATAAAATCAC GTTATACCAA GATGACGAAA GTCTTCACTC ACCAAGGAAA AGTGGATCTG 
TATAGCAAAT TGGTGCACTC AGCTGAGACT GAGAAGAGGA AACTTCAGAT AAAAATCAAC 
GAGATGGACA CCATACTTAA GAAGATCAAT AACTGTCTCA CTGCAGTGGA AATAGAAACT 
AAGAATTTGG AGGCTGAAGA GAAAGATGAT GCCATGGAAG ATTGGGATTC TGAAATACGA 
GATGCAGAGA AAGAACTGGA AAGGCTGAAA ACTGAGGAAG AGGAGCTTCA AAGAAAGTTC 
TTAGAAGTAG AGACACAGAA AACGCAGACC CTTGCTCAGA TAGAATTTAT AAAAGAACAA 
ACAACTGAAA CAGAAGAATT ATTGGATCAA TTGAGTTTTT CTGAGTGGGA TATCATCGAG 
TGGAGTGATG AACAAGCGGT ATTTACCTTT GTTTATGATT CAATAGAACT TATCATCACT 
TTTGGAGAGC CTCAGGTTGG TCTCCCTTTC TTGGACAAGG CTTACAGGAC GATTAATGAA 
CTCAGTTTTC AGTCTCTGTT AGACGAAGAT AAAGCGCCTC CTTCCTCCCT CTTGGTCCAC 
AAGCTTATTT TCCAGTACAT TGAAGAGCAG GAGTTCTGGA AGAAGAAGTG CACAACACAG 
CATCAGGTGC CCCAGATGCT TCAAGAACTC TCATTGGTAG TTAACCATTG TAGGCTTCTT 
GGAGAAGAGA TTGAATTTTT AAAGCGATGG GGACCAAATT ATAGCCTAAT GCATATAAAT 
GTGAATAATA CTGAATTACG GCTTCTATTC TCCAGTTCTG CAGCATTTGC AAAGTTTGAG 
ATAACTTTGT TTCCCTCGGC CCATTATCCC TTGGTCCCAC TACCATTCAC CATTCAAAAT 
CACCTTGGGA AGACTGGCCA TGATGAAATT GCTGCCGTTA TCTCTAAAGT GCCCTTAGAG 
GAAAACTACC TGAAGAATGT AGTCAAGCAG ATTTACCAAG ATCTACTTAA GGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Knl1 ( XM_008762096.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008760318.1 Rattus norvegicus kinetochore scaffold 1 (Knl1), transcript variant X2, mRNA.

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CloneID	ORa46967
Clone ID Related Accession (Same CDS sequence)	XM_008762099.2 , XM_008762097.2 , XM_008762096.1
Accession Version	XM_008762096.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6417bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	protein CASC5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGGGA TATATTCAGA GGCTAATGAA GAGAATGACA ATACACAGAG GCCTGTTAGA 
AGACAGCATT CCTCAATTTT GAAACCCCCA AGGAGTCCTC TTCAGGACCT CAAATGTGGA 
AATGAAATAG TTCAGGAATC TAATGCTCCG AGAACCAGGA AAAGCTCTCG TCGGGTCAGC 
TTTGCAGATA CTATAAAAGT ATTCCAGACA GAGTCCCATA TGAAAACGGA AAAAAATTCA 
GAAATTTCAG AGACAGAAGC CAGAAAAAAT GTGTTGACCC TACAGAACAA GAATTTAGAT 
GATAACTATT GTGCAATTAC TGGAATGAAC ACTTTGCTTT GTGCTCCAAT TCAGACTCAG 
ATGCAACAAA AGGAGTTTTC AATTACAGAC TGCAACCCTG AAAGGAAACA TGCAAATGAT 
CAAACAGTCA TCTTTTCAGA TGAAAATCAG ATGGATCTGA CAGCAAGTCA CACGGTGATG 
ATTGCCAAAG GTCTTGTAGA CTGTACCAAA CATGAAAATT CCACAAAAAT AGATACCACA 
TCCTTTCTGG AGAATTTAAA GCATCGTGCT GCGAATTCAA GAGTTAAAAA CGATTTAGCA 
TGTCCAATAA TTTCTATAAA TCAAAACATT TTTTCAGAAA AGAAAAATTC TGACAACTTC 
ATAAAAAGAT TGAAAATGGG GAAATATAAT ACTTCTTCTA CAGAACTTGA TAAGGAAAAT 
GCTGAAATAC CTGTTCATTC CAAAGACTCA AACAGTGCCT CTTCCACGTG TCAAATACAT 
GCATCGCTTA GTGTCGATGA ACATAGTAGT AATAAGACTA GACTCTTTAC AGAACAAGAT 
GATGGGATGA ATTTGACCCA GTGTCATACA GCCTGTATTC AGACTTGGAT TCCTCCATCC 
AGGGAGGCCA CCTTGGGAGA GGTTAAAGGT GATAATACAA TTTATGGCAA TGAGTGTATG 
GAGTTGACAA CTAACTACAC GATACAGGTG TTGTCATCAG AAAATAACTT ACCTGAAAGA 
GAAAATCAAG CTCAAAATGA TGTGATTGAT GTTACAACAG TCGATGGAGC TACAGCTCCA 
GAGAAAAAAA CAGCCCTTAA GGATAAACTA AATGCTGCTT TTCACAGCTC TTTCCCAAAC 
CCTGAAAATA AACTTCATAT TACCAAATGT CATCCTATAG AGTCAGAAAC TCATACAGTC 
ACACAGATTT CTAATCAAAA TGCCAACACA TTGGCTATGA CCTCAGAATC TGTATGTTCT 
AGCCCAGCTA TTCAAGGGTA TAAAACAATT TTTTACTCTA GTAGCAATGA TGCAATGGAA 
CTGACTAAGT GTCTATCAAT TATGCAAGAG GAAAAAAAAT TGCTGAAGAC TGATGATAAG 
TATTCTAAAA TATGTACCAA TTCAGATGCT AGCCCACTCA GAGAGAAAAC TATTTATTCA 
GAAGAGGATA GCATGGACAT TACCAAAAGT CACACAGTTG CAATAGATAA TAAGATATTT 
AAACATGATC AAGAAAACAT TAAAAAAGAA ATAACAGCTC TACCAATATT TGAAAAAGAA 
ATGATGCTCC AAAATCTTAA AACTATGTCC AAAGATGAGA AAATGAATGT GAATTCTATT 
ACAGTTCCTC AAGTATCTAA AGAAAGTTTA CAACAGAGTC TGACAAATGC CTTATCTATT 
TCATTGGCTG ATAAAACAAT GGAATTCTTA GCAGGTGAAG ATATGGATTT GACTAAAAGT 
CACACAACTA AGTTAAGTCA AGTTATTCCT ACAACTTTTG ACTTAGCATC AAAGAATGTC 
ACCAAATCTT CCTCTCACAG CAAAAGCCCT TCAGATGAGT GGGAAGGCCT GGAAAAACAA 
ATTGTGCTAG GCCAGCATTC TAAACTTCCT TCACCACAGA GGAAAGATTT AGATGATCCT 
GACTGTTCTC ATCATAAGAT AATGTATTCA GAGGAGGTAC AAACTATGGA TCTAACCAAG 
AGCCACACTA TTGTGATTGG GTTTGGTTCT TCTGAGGTAC AAGAACACAG TAAGATTAAT 
TTAGAACACA AAAGCAGCCA ACTTACAGCA GAAAACAGAC AAACAGCTGT GAACATTCCT 
GGAATAAACT TTAGAGTAGT AACAACTAAT GATATGGACA TGTTAAAGGA CAGAAGTATA 
CATAAGCCAG AATTGCTGGA GGAAAAGCAA AACGTCAAAA TTTATGGAAG AAAAAGTATG 
GGTGGACTGA AGATTGATAA AACTATATTA TTTTCAGAGG GTAATGAGGA TGATATGGAT 
ATCACTAAGA GTTGCACAGT CAAAATAAAC CATCGGTCTT TATTAGATAA ACATGATTCT 
CATTTAGTAT CTTTGGCAGG AACTTCTAAA ACCATTTTGC ATGCACGTGG ACAGGATGAA 
ATGGAAATTA ATAGAAATCA CACGACTCCT TTAGAGTGTA AAATTATCTC ACCCAGTGAT 
ATAACTTCTG GTGATCTGAG TAAGACTGTG ATGTCTATAG ATGATCATGA GGAACTAGAA 
ATGACTAAGT CCCATACTGT TTTCATTGAC TACCAAGCAG AGGCAAAAGG TGTACTTCCA 
GACAGACTTG ACTTTCAATT ATCCAAAAAG AAAAGCCTAC AGAAGCCAAA AATGACATCT 
ACTCATGCTG AAGAGATTGT TTCCATTTCA AAAAATAGTG AAAGCAACTA TCTACCAGCC 
AAAGGCAGCC AGGTAACAAT ATTAGAGGAA GGATCTAATA GTGGGCCTGG GGAAGAAACT 
AATGATGCAC AAAAGCCTGG ATTTCTGAAT GAACCTCTGT CAGGCAAAAC TCAAAGAAGA 
GAAAGCCTTA AGCTCAAAAA TAAGACTATT ACATTTTCAG GAAATGATAA AAGTAATAGA 
GATATCACTC AGAGTTCTGT GGTAGAAATA AATAATGAAA CTAACCTGGA AGATAGAAAG 
GGCTTCAGTT TTGTGCCATT GGCAGGAACT TCCAAGCCAG TTTTATCTGC ATATCGGCCA 
GAGGACATGG AGATCTCTAT AAGTCAAACC TCTGCTTCGG AGTATAAAAC TATCCCACCA 
GATGAAATGA CTACTATACC TATGGATAAA ACTGTAATGT TTGTAGATAA TCTCAGTGAT 
CTGGGTGTCA CCAGATCCCA TACGTTTTTC ATCGATTGTC AAGCCAAAGA GAAAGTACTT 
CATGAATATA CCAACTTGGG AATACCAAAA CCACAAAACT TGAGTGGTTC TGAAGATAAT 
TCCTGTATTC AAGAAATCAC CAAAAAACCA GTAGCTGAAT ACAAACACCA TATGACAACT 
GTTATTCCTT CTAATACATT ATTTAGTGAT CAAAGTACAA TGAAAATCAA GATGCATAAA 
GCTGATATAG ATGAAGAGGG CATAGGAAAG GAAGTGGAGG CCGATACATT GAAACAAACT 
AAACTTGAAA GGTGTCTGTT AAATAGAACT GATGGAAAAA ATGTGGATTT TACAAGTAGT 
CATATAGCTG ATGTTTGTAC ATCCAGAGAC AAGTATTCCT CTTTACCAAA TGTTATTTCT 
TCTTCTGATA ATTTAGATAG TAATACTAAG TCCTTGTGTG ATAAAAGTAG GGAGAAAGCT 
TATAATTGCC AAGTGCCAAA TGAGTTTACT TTTGCAGATA ATTTACCAAG TAGATATCAC 
CTGGATTCTG AAATACCATC TGTCTTTGTA TCATGTCCTT CAAAAGAAGT TACTCAAACT 
GAAAGTGCCA TAGCACTACT TCAAGATCAA GATCAAGAGC CTCTGGGACT CTCAAAGTGT 
GCATCTCTTA AGTGTGCAAA GGATCAAATG GAGGCCTTTG TTGATGTTAG TGTTGCTTCT 
CAGCCTCATC TCTCAGCCCA ACAACCTCCA TCAACTCAAA AAGGGCAGGA TGCCGCCAAT 
AGAGATGAAG GAATGCTATC TAAAGCTGGG AAGAAGACTT TACCTTTTCT TATAGGAAAT 
GTCTCTGCAC CCACATGTGA AAATGCGTCC AAAATGCCTA CTAATGAGGA ACACTTTGCT 
GTAACCTATA ACAAAGAACT GGAAAAAAGT ATTCAAACAG ATAAATGTAA TACAAATGTA 
CACAACCATA GTAACTCAGT CCTGACAACA CAAGTCATTC AAACACATGC CAGTGCCGAA 
GCAGCATCAG ACTCTATAGT TCCATCTAAT GGTTCCTGTT TTAGTAGTGC CAAACCAAGT 
CTGAGTAATT TGAATAGAAA AACTGGGAAG GTTTTAGACT TTAATACTGT TCATATATTA 
CCTCCTGCAG AACAGTTACT TGAAGCAGGA AATCAGGCAC ATAGTATGAG TATAGTACAA 
GGTACAGAAA TACATAGCCT AGGTTCCAGC AATGACAGAG ATAAAGAAAG TAAAACTCTG 
CATAATGAGG CCGAAGCTAC ATCTGTACCA CTAAAAGCAG TTGTTAAAGA CAAAGCAAGA 
AGATGTTCAT TGGGAATATT TTTACCCAAA TTACCAAACA AGAAAAGTTG TAGTATCACT 
GGTGTTGATG ATGTGGAACA GATTCTAGCA GATGCAACTG ACTTAACTCA CCTAGAAACT 
CAGCCAGTCT GTAGTCAAGA TCCGGGCATT GGCTCTGTTG CAGCCAAACT GAACTTGAGT 
CCATCTCAGT ATATAAATGA GGAGAATCTC CCTGTATATC CCGGTGAGAT ACTTTCATCA 
GATTCTGTCA TCCTGGACAT TGAGGAAAGT GCTCCGATCG ACACATATCA TAAAGAGATT 
TTACCATCTG AAAACAAAAC AGAAACCTGC AGGGCCCAAA AAAGAGCCTG GGTTCAAGAA 
AATGAGGTTA AAAATGAGAA AAAAATCAGA ACACATGATG GTGCACAAGA TCAAGAGATT 
TTTGATCATT ATACTGAAGA GGACATCAAT AAAAGTATTA ACAATGTATT TCTAAAAAGC 
CTGAGCAGGA CACCGTCTAG TTCTAGCAGC TCTCTGGATT CAATCAAGGC TGATGGAATC 
TCTCTGGATG CCAGCACACA GCGTAATAGC CAAATGGAAT CACAGTTTCT TGGTGACACT 
ATCTGTGAAG AGAGTTTGAG GGAGAAACTG AAGGATGGAC AAATTACAAT CAAAGAGTTC 
TTCATACTTC TTCAAGTTCA TATCTTAATA CAGAAACCTA GGCAGAGCAC TCTCCCAGCC 
AAATTTACTA TAAACACATT ACCTACCACA GAAGATCTGA TGTTGAGACA ATATGTTTAC 
GGACCCAAGA TACAAATTTA CAGAGAAGAT TGTGAGCTTC TTCGACAGAA GATTGAAGAA 
TTGAAGGTAT CTGCGTTGAA CCAAGATAAA CTATTGGTGG ATGTAAATAG GAACTTGTGG 
GAAAAAATGA AAGACTACTC TGACGAAGAG CTGAAGACCT ATGGAATTTA CCTTAACAAA 
ATAAAATCAC GTTATACCAA GATGACGAAA GTCTTCACTC ACCAAGGAAA AGTGGATCTG 
TATAGCAAAT TGGTGCACTC AGCTGAGACT GAGAAGAGGA AACTTCAGAT AAAAATCAAC 
GAGATGGACA CCATACTTAA GAAGATCAAT AACTGTCTCA CTGCAGTGGA AATAGAAACT 
AAGAATTTGG AGGCTGAAGA GAAAGATGAT GCCATGGAAG ATTGGGATTC TGAAATACGA 
GATGCAGAGA AAGAACTGGA AAGGCTGAAA ACTGAGGAAG AGGAGCTTCA AAGAAAGTTC 
TTAGAAGTAG AGACACAGAA AACGCAGACC CTTGCTCAGA TAGAATTTAT AAAAGAACAA 
ACAACTGAAA CAGAAGAATT ATTGGATCAA TTGAGTTTTT CTGAGTGGGA TATCATCGAG 
TGGAGTGATG AACAAGCGGT ATTTACCTTT GTTTATGATT CAATAGAACT TATCATCACT 
TTTGGAGAGC CTCAGGTTGG TCTCCCTTTC TTGGACAAGG CTTACAGGAC GATTAATGAA 
CTCAGTTTTC AGTCTCTGTT AGACGAAGAT AAAGCGCCTC CTTCCTCCCT CTTGGTCCAC 
AAGCTTATTT TCCAGTACAT TGAAGAGCAG GAGTTCTGGA AGAAGAAGTG CACAACACAG 
CATCAGGTGC CCCAGATGCT TCAAGAACTC TCATTGGTAG TTAACCATTG TAGGCTTCTT 
GGAGAAGAGA TTGAATTTTT AAAGCGATGG GGACCAAATT ATAGCCTAAT GCATATAAAT 
GTGAATAATA CTGAATTACG GCTTCTATTC TCCAGTTCTG CAGCATTTGC AAAGTTTGAG 
ATAACTTTGT TTCCCTCGGC CCATTATCCC TTGGTCCCAC TACCATTCAC CATTCAAAAT 
CACCTTGGGA AGACTGGCCA TGATGAAATT GCTGCCGTTA TCTCTAAAGT GCCCTTAGAG 
GAAAACTACC TGAAGAATGT AGTCAAGCAG ATTTACCAAG ATCTACTTAA GGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008760318.1
CDS	345..6761
Translation	MDGIYSEANEENDNTQRPVRRQHSSILKPPRSPLQDLKCGNEIVQESNAPRTRKSSRRVSFADTIKVFQTESHMKTEKNSEISETEARKNVLTLQNKNLDDNYCAITGMNTLLCAPIQTQMQQKEFSITDCNPERKHANDQTVIFSDENQMDLTASHTVMIAKGLVDCTKHENSTKIDTTSFLENLKHRAANSRVKNDLACPIISINQNIFSEKKNSDNFIKRLKMGKYNTSSTELDKENAEIPVHSKDSNSASSTCQIHASLSVDEHSSNKTRLFTEQDDGMNLTQCHTACIQTWIPPSREATLGEVKGDNTIYGNECMELTTNYTIQVLSSENNLPERENQAQNDVIDVTTVDGATAPEKKTALKDKLNAAFHSSFPNPENKLHITKCHPIESETHTVTQISNQNANTLAMTSESVCSSPAIQGYKTIFYSSSNDAMELTKCLSIMQEEKKLLKTDDKYSKICTNSDASPLREKTIYSEEDSMDITKSHTVAIDNKIFKHDQENIKKEITALPIFEKEMMLQNLKTMSKDEKMNVNSITVPQVSKESLQQSLTNALSISLADKTMEFLAGEDMDLTKSHTTKLSQVIPTTFDLASKNVTKSSSHSKSPSDEWEGLEKQIVLGQHSKLPSPQRKDLDDPDCSHHKIMYSEEVQTMDLTKSHTIVIGFGSSEVQEHSKINLEHKSSQLTAENRQTAVNIPGINFRVVTTNDMDMLKDRSIHKPELLEEKQNVKIYGRKSMGGLKIDKTILFSEGNEDDMDITKSCTVKINHRSLLDKHDSHLVSLAGTSKTILHARGQDEMEINRNHTTPLECKIISPSDITSGDLSKTVMSIDDHEELEMTKSHTVFIDYQAEAKGVLPDRLDFQLSKKKSLQKPKMTSTHAEEIVSISKNSESNYLPAKGSQVTILEEGSNSGPGEETNDAQKPGFLNEPLSGKTQRRESLKLKNKTITFSGNDKSNRDITQSSVVEINNETNLEDRKGFSFVPLAGTSKPVLSAYRPEDMEISISQTSASEYKTIPPDEMTTIPMDKTVMFVDNLSDLGVTRSHTFFIDCQAKEKVLHEYTNLGIPKPQNLSGSEDNSCIQEITKKPVAEYKHHMTTVIPSNTLFSDQSTMKIKMHKADIDEEGIGKEVEADTLKQTKLERCLLNRTDGKNVDFTSSHIADVCTSRDKYSSLPNVISSSDNLDSNTKSLCDKSREKAYNCQVPNEFTFADNLPSRYHLDSEIPSVFVSCPSKEVTQTESAIALLQDQDQEPLGLSKCASLKCAKDQMEAFVDVSVASQPHLSAQQPPSTQKGQDAANRDEGMLSKAGKKTLPFLIGNVSAPTCENASKMPTNEEHFAVTYNKELEKSIQTDKCNTNVHNHSNSVLTTQVIQTHASAEAASDSIVPSNGSCFSSAKPSLSNLNRKTGKVLDFNTVHILPPAEQLLEAGNQAHSMSIVQGTEIHSLGSSNDRDKESKTLHNEAEATSVPLKAVVKDKARRCSLGIFLPKLPNKKSCSITGVDDVEQILADATDLTHLETQPVCSQDPGIGSVAAKLNLSPSQYINEENLPVYPGEILSSDSVILDIEESAPIDTYHKEILPSENKTETCRAQKRAWVQENEVKNEKKIRTHDGAQDQEIFDHYTEEDINKSINNVFLKSLSRTPSSSSSSLDSIKADGISLDASTQRNSQMESQFLGDTICEESLREKLKDGQITIKEFFILLQVHILIQKPRQSTLPAKFTINTLPTTEDLMLRQYVYGPKIQIYREDCELLRQKIEELKVSALNQDKLLVDVNRNLWEKMKDYSDEELKTYGIYLNKIKSRYTKMTKVFTHQGKVDLYSKLVHSAETEKRKLQIKINEMDTILKKINNCLTAVEIETKNLEAEEKDDAMEDWDSEIRDAEKELERLKTEEEELQRKFLEVETQKTQTLAQIEFIKEQTTETEELLDQLSFSEWDIIEWSDEQAVFTFVYDSIELIITFGEPQVGLPFLDKAYRTINELSFQSLLDEDKAPPSSLLVHKLIFQYIEEQEFWKKKCTTQHQVPQMLQELSLVVNHCRLLGEEIEFLKRWGPNYSLMHINVNNTELRLLFSSSAAFAKFEITLFPSAHYPLVPLPFTIQNHLGKTGHDEIAAVISKVPLEENYLKNVVKQIYQDLLKD

Target ORF information:

RefSeq Version	XM_008762096.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus kinetochore scaffold 1 (Knl1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008762096.1

ORF Insert Sequence:

ATGGATGGGA TATATTCAGA GGCTAATGAA GAGAATGACA ATACACAGAG GCCTGTTAGA 
AGACAGCATT CCTCAATTTT GAAACCCCCA AGGAGTCCTC TTCAGGACCT CAAATGTGGA 
AATGAAATAG TTCAGGAATC TAATGCTCCG AGAACCAGGA AAAGCTCTCG TCGGGTCAGC 
TTTGCAGATA CTATAAAAGT ATTCCAGACA GAGTCCCATA TGAAAACGGA AAAAAATTCA 
GAAATTTCAG AGACAGAAGC CAGAAAAAAT GTGTTGACCC TACAGAACAA GAATTTAGAT 
GATAACTATT GTGCAATTAC TGGAATGAAC ACTTTGCTTT GTGCTCCAAT TCAGACTCAG 
ATGCAACAAA AGGAGTTTTC AATTACAGAC TGCAACCCTG AAAGGAAACA TGCAAATGAT 
CAAACAGTCA TCTTTTCAGA TGAAAATCAG ATGGATCTGA CAGCAAGTCA CACGGTGATG 
ATTGCCAAAG GTCTTGTAGA CTGTACCAAA CATGAAAATT CCACAAAAAT AGATACCACA 
TCCTTTCTGG AGAATTTAAA GCATCGTGCT GCGAATTCAA GAGTTAAAAA CGATTTAGCA 
TGTCCAATAA TTTCTATAAA TCAAAACATT TTTTCAGAAA AGAAAAATTC TGACAACTTC 
ATAAAAAGAT TGAAAATGGG GAAATATAAT ACTTCTTCTA CAGAACTTGA TAAGGAAAAT 
GCTGAAATAC CTGTTCATTC CAAAGACTCA AACAGTGCCT CTTCCACGTG TCAAATACAT 
GCATCGCTTA GTGTCGATGA ACATAGTAGT AATAAGACTA GACTCTTTAC AGAACAAGAT 
GATGGGATGA ATTTGACCCA GTGTCATACA GCCTGTATTC AGACTTGGAT TCCTCCATCC 
AGGGAGGCCA CCTTGGGAGA GGTTAAAGGT GATAATACAA TTTATGGCAA TGAGTGTATG 
GAGTTGACAA CTAACTACAC GATACAGGTG TTGTCATCAG AAAATAACTT ACCTGAAAGA 
GAAAATCAAG CTCAAAATGA TGTGATTGAT GTTACAACAG TCGATGGAGC TACAGCTCCA 
GAGAAAAAAA CAGCCCTTAA GGATAAACTA AATGCTGCTT TTCACAGCTC TTTCCCAAAC 
CCTGAAAATA AACTTCATAT TACCAAATGT CATCCTATAG AGTCAGAAAC TCATACAGTC 
ACACAGATTT CTAATCAAAA TGCCAACACA TTGGCTATGA CCTCAGAATC TGTATGTTCT 
AGCCCAGCTA TTCAAGGGTA TAAAACAATT TTTTACTCTA GTAGCAATGA TGCAATGGAA 
CTGACTAAGT GTCTATCAAT TATGCAAGAG GAAAAAAAAT TGCTGAAGAC TGATGATAAG 
TATTCTAAAA TATGTACCAA TTCAGATGCT AGCCCACTCA GAGAGAAAAC TATTTATTCA 
GAAGAGGATA GCATGGACAT TACCAAAAGT CACACAGTTG CAATAGATAA TAAGATATTT 
AAACATGATC AAGAAAACAT TAAAAAAGAA ATAACAGCTC TACCAATATT TGAAAAAGAA 
ATGATGCTCC AAAATCTTAA AACTATGTCC AAAGATGAGA AAATGAATGT GAATTCTATT 
ACAGTTCCTC AAGTATCTAA AGAAAGTTTA CAACAGAGTC TGACAAATGC CTTATCTATT 
TCATTGGCTG ATAAAACAAT GGAATTCTTA GCAGGTGAAG ATATGGATTT GACTAAAAGT 
CACACAACTA AGTTAAGTCA AGTTATTCCT ACAACTTTTG ACTTAGCATC AAAGAATGTC 
ACCAAATCTT CCTCTCACAG CAAAAGCCCT TCAGATGAGT GGGAAGGCCT GGAAAAACAA 
ATTGTGCTAG GCCAGCATTC TAAACTTCCT TCACCACAGA GGAAAGATTT AGATGATCCT 
GACTGTTCTC ATCATAAGAT AATGTATTCA GAGGAGGTAC AAACTATGGA TCTAACCAAG 
AGCCACACTA TTGTGATTGG GTTTGGTTCT TCTGAGGTAC AAGAACACAG TAAGATTAAT 
TTAGAACACA AAAGCAGCCA ACTTACAGCA GAAAACAGAC AAACAGCTGT GAACATTCCT 
GGAATAAACT TTAGAGTAGT AACAACTAAT GATATGGACA TGTTAAAGGA CAGAAGTATA 
CATAAGCCAG AATTGCTGGA GGAAAAGCAA AACGTCAAAA TTTATGGAAG AAAAAGTATG 
GGTGGACTGA AGATTGATAA AACTATATTA TTTTCAGAGG GTAATGAGGA TGATATGGAT 
ATCACTAAGA GTTGCACAGT CAAAATAAAC CATCGGTCTT TATTAGATAA ACATGATTCT 
CATTTAGTAT CTTTGGCAGG AACTTCTAAA ACCATTTTGC ATGCACGTGG ACAGGATGAA 
ATGGAAATTA ATAGAAATCA CACGACTCCT TTAGAGTGTA AAATTATCTC ACCCAGTGAT 
ATAACTTCTG GTGATCTGAG TAAGACTGTG ATGTCTATAG ATGATCATGA GGAACTAGAA 
ATGACTAAGT CCCATACTGT TTTCATTGAC TACCAAGCAG AGGCAAAAGG TGTACTTCCA 
GACAGACTTG ACTTTCAATT ATCCAAAAAG AAAAGCCTAC AGAAGCCAAA AATGACATCT 
ACTCATGCTG AAGAGATTGT TTCCATTTCA AAAAATAGTG AAAGCAACTA TCTACCAGCC 
AAAGGCAGCC AGGTAACAAT ATTAGAGGAA GGATCTAATA GTGGGCCTGG GGAAGAAACT 
AATGATGCAC AAAAGCCTGG ATTTCTGAAT GAACCTCTGT CAGGCAAAAC TCAAAGAAGA 
GAAAGCCTTA AGCTCAAAAA TAAGACTATT ACATTTTCAG GAAATGATAA AAGTAATAGA 
GATATCACTC AGAGTTCTGT GGTAGAAATA AATAATGAAA CTAACCTGGA AGATAGAAAG 
GGCTTCAGTT TTGTGCCATT GGCAGGAACT TCCAAGCCAG TTTTATCTGC ATATCGGCCA 
GAGGACATGG AGATCTCTAT AAGTCAAACC TCTGCTTCGG AGTATAAAAC TATCCCACCA 
GATGAAATGA CTACTATACC TATGGATAAA ACTGTAATGT TTGTAGATAA TCTCAGTGAT 
CTGGGTGTCA CCAGATCCCA TACGTTTTTC ATCGATTGTC AAGCCAAAGA GAAAGTACTT 
CATGAATATA CCAACTTGGG AATACCAAAA CCACAAAACT TGAGTGGTTC TGAAGATAAT 
TCCTGTATTC AAGAAATCAC CAAAAAACCA GTAGCTGAAT ACAAACACCA TATGACAACT 
GTTATTCCTT CTAATACATT ATTTAGTGAT CAAAGTACAA TGAAAATCAA GATGCATAAA 
GCTGATATAG ATGAAGAGGG CATAGGAAAG GAAGTGGAGG CCGATACATT GAAACAAACT 
AAACTTGAAA GGTGTCTGTT AAATAGAACT GATGGAAAAA ATGTGGATTT TACAAGTAGT 
CATATAGCTG ATGTTTGTAC ATCCAGAGAC AAGTATTCCT CTTTACCAAA TGTTATTTCT 
TCTTCTGATA ATTTAGATAG TAATACTAAG TCCTTGTGTG ATAAAAGTAG GGAGAAAGCT 
TATAATTGCC AAGTGCCAAA TGAGTTTACT TTTGCAGATA ATTTACCAAG TAGATATCAC 
CTGGATTCTG AAATACCATC TGTCTTTGTA TCATGTCCTT CAAAAGAAGT TACTCAAACT 
GAAAGTGCCA TAGCACTACT TCAAGATCAA GATCAAGAGC CTCTGGGACT CTCAAAGTGT 
GCATCTCTTA AGTGTGCAAA GGATCAAATG GAGGCCTTTG TTGATGTTAG TGTTGCTTCT 
CAGCCTCATC TCTCAGCCCA ACAACCTCCA TCAACTCAAA AAGGGCAGGA TGCCGCCAAT 
AGAGATGAAG GAATGCTATC TAAAGCTGGG AAGAAGACTT TACCTTTTCT TATAGGAAAT 
GTCTCTGCAC CCACATGTGA AAATGCGTCC AAAATGCCTA CTAATGAGGA ACACTTTGCT 
GTAACCTATA ACAAAGAACT GGAAAAAAGT ATTCAAACAG ATAAATGTAA TACAAATGTA 
CACAACCATA GTAACTCAGT CCTGACAACA CAAGTCATTC AAACACATGC CAGTGCCGAA 
GCAGCATCAG ACTCTATAGT TCCATCTAAT GGTTCCTGTT TTAGTAGTGC CAAACCAAGT 
CTGAGTAATT TGAATAGAAA AACTGGGAAG GTTTTAGACT TTAATACTGT TCATATATTA 
CCTCCTGCAG AACAGTTACT TGAAGCAGGA AATCAGGCAC ATAGTATGAG TATAGTACAA 
GGTACAGAAA TACATAGCCT AGGTTCCAGC AATGACAGAG ATAAAGAAAG TAAAACTCTG 
CATAATGAGG CCGAAGCTAC ATCTGTACCA CTAAAAGCAG TTGTTAAAGA CAAAGCAAGA 
AGATGTTCAT TGGGAATATT TTTACCCAAA TTACCAAACA AGAAAAGTTG TAGTATCACT 
GGTGTTGATG ATGTGGAACA GATTCTAGCA GATGCAACTG ACTTAACTCA CCTAGAAACT 
CAGCCAGTCT GTAGTCAAGA TCCGGGCATT GGCTCTGTTG CAGCCAAACT GAACTTGAGT 
CCATCTCAGT ATATAAATGA GGAGAATCTC CCTGTATATC CCGGTGAGAT ACTTTCATCA 
GATTCTGTCA TCCTGGACAT TGAGGAAAGT GCTCCGATCG ACACATATCA TAAAGAGATT 
TTACCATCTG AAAACAAAAC AGAAACCTGC AGGGCCCAAA AAAGAGCCTG GGTTCAAGAA 
AATGAGGTTA AAAATGAGAA AAAAATCAGA ACACATGATG GTGCACAAGA TCAAGAGATT 
TTTGATCATT ATACTGAAGA GGACATCAAT AAAAGTATTA ACAATGTATT TCTAAAAAGC 
CTGAGCAGGA CACCGTCTAG TTCTAGCAGC TCTCTGGATT CAATCAAGGC TGATGGAATC 
TCTCTGGATG CCAGCACACA GCGTAATAGC CAAATGGAAT CACAGTTTCT TGGTGACACT 
ATCTGTGAAG AGAGTTTGAG GGAGAAACTG AAGGATGGAC AAATTACAAT CAAAGAGTTC 
TTCATACTTC TTCAAGTTCA TATCTTAATA CAGAAACCTA GGCAGAGCAC TCTCCCAGCC 
AAATTTACTA TAAACACATT ACCTACCACA GAAGATCTGA TGTTGAGACA ATATGTTTAC 
GGACCCAAGA TACAAATTTA CAGAGAAGAT TGTGAGCTTC TTCGACAGAA GATTGAAGAA 
TTGAAGGTAT CTGCGTTGAA CCAAGATAAA CTATTGGTGG ATGTAAATAG GAACTTGTGG 
GAAAAAATGA AAGACTACTC TGACGAAGAG CTGAAGACCT ATGGAATTTA CCTTAACAAA 
ATAAAATCAC GTTATACCAA GATGACGAAA GTCTTCACTC ACCAAGGAAA AGTGGATCTG 
TATAGCAAAT TGGTGCACTC AGCTGAGACT GAGAAGAGGA AACTTCAGAT AAAAATCAAC 
GAGATGGACA CCATACTTAA GAAGATCAAT AACTGTCTCA CTGCAGTGGA AATAGAAACT 
AAGAATTTGG AGGCTGAAGA GAAAGATGAT GCCATGGAAG ATTGGGATTC TGAAATACGA 
GATGCAGAGA AAGAACTGGA AAGGCTGAAA ACTGAGGAAG AGGAGCTTCA AAGAAAGTTC 
TTAGAAGTAG AGACACAGAA AACGCAGACC CTTGCTCAGA TAGAATTTAT AAAAGAACAA 
ACAACTGAAA CAGAAGAATT ATTGGATCAA TTGAGTTTTT CTGAGTGGGA TATCATCGAG 
TGGAGTGATG AACAAGCGGT ATTTACCTTT GTTTATGATT CAATAGAACT TATCATCACT 
TTTGGAGAGC CTCAGGTTGG TCTCCCTTTC TTGGACAAGG CTTACAGGAC GATTAATGAA 
CTCAGTTTTC AGTCTCTGTT AGACGAAGAT AAAGCGCCTC CTTCCTCCCT CTTGGTCCAC 
AAGCTTATTT TCCAGTACAT TGAAGAGCAG GAGTTCTGGA AGAAGAAGTG CACAACACAG 
CATCAGGTGC CCCAGATGCT TCAAGAACTC TCATTGGTAG TTAACCATTG TAGGCTTCTT 
GGAGAAGAGA TTGAATTTTT AAAGCGATGG GGACCAAATT ATAGCCTAAT GCATATAAAT 
GTGAATAATA CTGAATTACG GCTTCTATTC TCCAGTTCTG CAGCATTTGC AAAGTTTGAG 
ATAACTTTGT TTCCCTCGGC CCATTATCCC TTGGTCCCAC TACCATTCAC CATTCAAAAT 
CACCTTGGGA AGACTGGCCA TGATGAAATT GCTGCCGTTA TCTCTAAAGT GCCCTTAGAG 
GAAAACTACC TGAAGAATGT AGTCAAGCAG ATTTACCAAG ATCTACTTAA GGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Knl1 ( XM_008762098.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008760320.1 Rattus norvegicus kinetochore scaffold 1 (Knl1), transcript variant X4, mRNA.

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CloneID	ORa46968
Clone ID Related Accession (Same CDS sequence)	XM_008762098.1
Accession Version	XM_008762098.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6384bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	protein CASC5 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGAGGA GCGCGCGGAC CCCAGACAAG TCAAGCAGAA AAACGTTTTC AAAAATGGAT 
GGGATATATT CAGAGGCTAA TGAAGAGAAT GACAATACAC AGAGGCCTGT TAGAAGACAG 
CATTCCTCAA TTTTGAAACC CCCAAGGAGT CCTCTTCAGG ACCTCAAATG TGGAAATGAA 
ATAGTTCAGG AATCTAATGC TCCGAGAACC AGGAAAAGCT CTCGTCGGGT CAGCTTTGCA 
GATACTATAA AGAACAAGAA TTTAGATGAT AACTATTGTG CAATTACTGG AATGAACACT 
TTGCTTTGTG CTCCAATTCA GACTCAGATG CAACAAAAGG AGTTTTCAAT TACAGACTGC 
AACCCTGAAA GGAAACATGC AAATGATCAA ACAGTCATCT TTTCAGATGA AAATCAGATG 
GATCTGACAG CAAGTCACAC GGTGATGATT GCCAAAGGTC TTGTAGACTG TACCAAACAT 
GAAAATTCCA CAAAAATAGA TACCACATCC TTTCTGGAGA ATTTAAAGCA TCGTGCTGCG 
AATTCAAGAG TTAAAAACGA TTTAGCATGT CCAATAATTT CTATAAATCA AAACATTTTT 
TCAGAAAAGA AAAATTCTGA CAACTTCATA AAAAGATTGA AAATGGGGAA ATATAATACT 
TCTTCTACAG AACTTGATAA GGAAAATGCT GAAATACCTG TTCATTCCAA AGACTCAAAC 
AGTGCCTCTT CCACGTGTCA AATACATGCA TCGCTTAGTG TCGATGAACA TAGTAGTAAT 
AAGACTAGAC TCTTTACAGA ACAAGATGAT GGGATGAATT TGACCCAGTG TCATACAGCC 
TGTATTCAGA CTTGGATTCC TCCATCCAGG GAGGCCACCT TGGGAGAGGT TAAAGGTGAT 
AATACAATTT ATGGCAATGA GTGTATGGAG TTGACAACTA ACTACACGAT ACAGGTGTTG 
TCATCAGAAA ATAACTTACC TGAAAGAGAA AATCAAGCTC AAAATGATGT GATTGATGTT 
ACAACAGTCG ATGGAGCTAC AGCTCCAGAG AAAAAAACAG CCCTTAAGGA TAAACTAAAT 
GCTGCTTTTC ACAGCTCTTT CCCAAACCCT GAAAATAAAC TTCATATTAC CAAATGTCAT 
CCTATAGAGT CAGAAACTCA TACAGTCACA CAGATTTCTA ATCAAAATGC CAACACATTG 
GCTATGACCT CAGAATCTGT ATGTTCTAGC CCAGCTATTC AAGGGTATAA AACAATTTTT 
TACTCTAGTA GCAATGATGC AATGGAACTG ACTAAGTGTC TATCAATTAT GCAAGAGGAA 
AAAAAATTGC TGAAGACTGA TGATAAGTAT TCTAAAATAT GTACCAATTC AGATGCTAGC 
CCACTCAGAG AGAAAACTAT TTATTCAGAA GAGGATAGCA TGGACATTAC CAAAAGTCAC 
ACAGTTGCAA TAGATAATAA GATATTTAAA CATGATCAAG AAAACATTAA AAAAGAAATA 
ACAGCTCTAC CAATATTTGA AAAAGAAATG ATGCTCCAAA ATCTTAAAAC TATGTCCAAA 
GATGAGAAAA TGAATGTGAA TTCTATTACA GTTCCTCAAG TATCTAAAGA AAGTTTACAA 
CAGAGTCTGA CAAATGCCTT ATCTATTTCA TTGGCTGATA AAACAATGGA ATTCTTAGCA 
GGTGAAGATA TGGATTTGAC TAAAAGTCAC ACAACTAAGT TAAGTCAAGT TATTCCTACA 
ACTTTTGACT TAGCATCAAA GAATGTCACC AAATCTTCCT CTCACAGCAA AAGCCCTTCA 
GATGAGTGGG AAGGCCTGGA AAAACAAATT GTGCTAGGCC AGCATTCTAA ACTTCCTTCA 
CCACAGAGGA AAGATTTAGA TGATCCTGAC TGTTCTCATC ATAAGATAAT GTATTCAGAG 
GAGGTACAAA CTATGGATCT AACCAAGAGC CACACTATTG TGATTGGGTT TGGTTCTTCT 
GAGGTACAAG AACACAGTAA GATTAATTTA GAACACAAAA GCAGCCAACT TACAGCAGAA 
AACAGACAAA CAGCTGTGAA CATTCCTGGA ATAAACTTTA GAGTAGTAAC AACTAATGAT 
ATGGACATGT TAAAGGACAG AAGTATACAT AAGCCAGAAT TGCTGGAGGA AAAGCAAAAC 
GTCAAAATTT ATGGAAGAAA AAGTATGGGT GGACTGAAGA TTGATAAAAC TATATTATTT 
TCAGAGGGTA ATGAGGATGA TATGGATATC ACTAAGAGTT GCACAGTCAA AATAAACCAT 
CGGTCTTTAT TAGATAAACA TGATTCTCAT TTAGTATCTT TGGCAGGAAC TTCTAAAACC 
ATTTTGCATG CACGTGGACA GGATGAAATG GAAATTAATA GAAATCACAC GACTCCTTTA 
GAGTGTAAAA TTATCTCACC CAGTGATATA ACTTCTGGTG ATCTGAGTAA GACTGTGATG 
TCTATAGATG ATCATGAGGA ACTAGAAATG ACTAAGTCCC ATACTGTTTT CATTGACTAC 
CAAGCAGAGG CAAAAGGTGT ACTTCCAGAC AGACTTGACT TTCAATTATC CAAAAAGAAA 
AGCCTACAGA AGCCAAAAAT GACATCTACT CATGCTGAAG AGATTGTTTC CATTTCAAAA 
AATAGTGAAA GCAACTATCT ACCAGCCAAA GGCAGCCAGG TAACAATATT AGAGGAAGGA 
TCTAATAGTG GGCCTGGGGA AGAAACTAAT GATGCACAAA AGCCTGGATT TCTGAATGAA 
CCTCTGTCAG GCAAAACTCA AAGAAGAGAA AGCCTTAAGC TCAAAAATAA GACTATTACA 
TTTTCAGGAA ATGATAAAAG TAATAGAGAT ATCACTCAGA GTTCTGTGGT AGAAATAAAT 
AATGAAACTA ACCTGGAAGA TAGAAAGGGC TTCAGTTTTG TGCCATTGGC AGGAACTTCC 
AAGCCAGTTT TATCTGCATA TCGGCCAGAG GACATGGAGA TCTCTATAAG TCAAACCTCT 
GCTTCGGAGT ATAAAACTAT CCCACCAGAT GAAATGACTA CTATACCTAT GGATAAAACT 
GTAATGTTTG TAGATAATCT CAGTGATCTG GGTGTCACCA GATCCCATAC GTTTTTCATC 
GATTGTCAAG CCAAAGAGAA AGTACTTCAT GAATATACCA ACTTGGGAAT ACCAAAACCA 
CAAAACTTGA GTGGTTCTGA AGATAATTCC TGTATTCAAG AAATCACCAA AAAACCAGTA 
GCTGAATACA AACACCATAT GACAACTGTT ATTCCTTCTA ATACATTATT TAGTGATCAA 
AGTACAATGA AAATCAAGAT GCATAAAGCT GATATAGATG AAGAGGGCAT AGGAAAGGAA 
GTGGAGGCCG ATACATTGAA ACAAACTAAA CTTGAAAGGT GTCTGTTAAA TAGAACTGAT 
GGAAAAAATG TGGATTTTAC AAGTAGTCAT ATAGCTGATG TTTGTACATC CAGAGACAAG 
TATTCCTCTT TACCAAATGT TATTTCTTCT TCTGATAATT TAGATAGTAA TACTAAGTCC 
TTGTGTGATA AAAGTAGGGA GAAAGCTTAT AATTGCCAAG TGCCAAATGA GTTTACTTTT 
GCAGATAATT TACCAAGTAG ATATCACCTG GATTCTGAAA TACCATCTGT CTTTGTATCA 
TGTCCTTCAA AAGAAGTTAC TCAAACTGAA AGTGCCATAG CACTACTTCA AGATCAAGAT 
CAAGAGCCTC TGGGACTCTC AAAGTGTGCA TCTCTTAAGT GTGCAAAGGA TCAAATGGAG 
GCCTTTGTTG ATGTTAGTGT TGCTTCTCAG CCTCATCTCT CAGCCCAACA ACCTCCATCA 
ACTCAAAAAG GGCAGGATGC CGCCAATAGA GATGAAGGAA TGCTATCTAA AGCTGGGAAG 
AAGACTTTAC CTTTTCTTAT AGGAAATGTC TCTGCACCCA CATGTGAAAA TGCGTCCAAA 
ATGCCTACTA ATGAGGAACA CTTTGCTGTA ACCTATAACA AAGAACTGGA AAAAAGTATT 
CAAACAGATA AATGTAATAC AAATGTACAC AACCATAGTA ACTCAGTCCT GACAACACAA 
GTCATTCAAA CACATGCCAG TGCCGAAGCA GCATCAGACT CTATAGTTCC ATCTAATGGT 
TCCTGTTTTA GTAGTGCCAA ACCAAGTCTG AGTAATTTGA ATAGAAAAAC TGGGAAGGTT 
TTAGACTTTA ATACTGTTCA TATATTACCT CCTGCAGAAC AGTTACTTGA AGCAGGAAAT 
CAGGCACATA GTATGAGTAT AGTACAAGGT ACAGAAATAC ATAGCCTAGG TTCCAGCAAT 
GACAGAGATA AAGAAAGTAA AACTCTGCAT AATGAGGCCG AAGCTACATC TGTACCACTA 
AAAGCAGTTG TTAAAGACAA AGCAAGAAGA TGTTCATTGG GAATATTTTT ACCCAAATTA 
CCAAACAAGA AAAGTTGTAG TATCACTGGT GTTGATGATG TGGAACAGAT TCTAGCAGAT 
GCAACTGACT TAACTCACCT AGAAACTCAG CCAGTCTGTA GTCAAGATCC GGGCATTGGC 
TCTGTTGCAG CCAAACTGAA CTTGAGTCCA TCTCAGTATA TAAATGAGGA GAATCTCCCT 
GTATATCCCG GTGAGATACT TTCATCAGAT TCTGTCATCC TGGACATTGA GGAAAGTGCT 
CCGATCGACA CATATCATAA AGAGATTTTA CCATCTGAAA ACAAAACAGA AACCTGCAGG 
GCCCAAAAAA GAGCCTGGGT TCAAGAAAAT GAGGTTAAAA ATGAGAAAAA AATCAGAACA 
CATGATGGTG CACAAGATCA AGAGATTTTT GATCATTATA CTGAAGAGGA CATCAATAAA 
AGTATTAACA ATGTATTTCT AAAAAGCCTG AGCAGGACAC CGTCTAGTTC TAGCAGCTCT 
CTGGATTCAA TCAAGGCTGA TGGAATCTCT CTGGATGCCA GCACACAGCG TAATAGCCAA 
ATGGAATCAC AGTTTCTTGG TGACACTATC TGTGAAGAGA GTTTGAGGGA GAAACTGAAG 
GATGGACAAA TTACAATCAA AGAGTTCTTC ATACTTCTTC AAGTTCATAT CTTAATACAG 
AAACCTAGGC AGAGCACTCT CCCAGCCAAA TTTACTATAA ACACATTACC TACCACAGAA 
GATCTGATGT TGAGACAATA TGTTTACGGA CCCAAGATAC AAATTTACAG AGAAGATTGT 
GAGCTTCTTC GACAGAAGAT TGAAGAATTG AAGGTATCTG CGTTGAACCA AGATAAACTA 
TTGGTGGATG TAAATAGGAA CTTGTGGGAA AAAATGAAAG ACTACTCTGA CGAAGAGCTG 
AAGACCTATG GAATTTACCT TAACAAAATA AAATCACGTT ATACCAAGAT GACGAAAGTC 
TTCACTCACC AAGGAAAAGT GGATCTGTAT AGCAAATTGG TGCACTCAGC TGAGACTGAG 
AAGAGGAAAC TTCAGATAAA AATCAACGAG ATGGACACCA TACTTAAGAA GATCAATAAC 
TGTCTCACTG CAGTGGAAAT AGAAACTAAG AATTTGGAGG CTGAAGAGAA AGATGATGCC 
ATGGAAGATT GGGATTCTGA AATACGAGAT GCAGAGAAAG AACTGGAAAG GCTGAAAACT 
GAGGAAGAGG AGCTTCAAAG AAAGTTCTTA GAAGTAGAGA CACAGAAAAC GCAGACCCTT 
GCTCAGATAG AATTTATAAA AGAACAAACA ACTGAAACAG AAGAATTATT GGATCAATTG 
AGTTTTTCTG AGTGGGATAT CATCGAGTGG AGTGATGAAC AAGCGGTATT TACCTTTGTT 
TATGATTCAA TAGAACTTAT CATCACTTTT GGAGAGCCTC AGGTTGGTCT CCCTTTCTTG 
GACAAGGCTT ACAGGACGAT TAATGAACTC AGTTTTCAGT CTCTGTTAGA CGAAGATAAA 
GCGCCTCCTT CCTCCCTCTT GGTCCACAAG CTTATTTTCC AGTACATTGA AGAGCAGGAG 
TTCTGGAAGA AGAAGTGCAC AACACAGCAT CAGGTGCCCC AGATGCTTCA AGAACTCTCA 
TTGGTAGTTA ACCATTGTAG GCTTCTTGGA GAAGAGATTG AATTTTTAAA GCGATGGGGA 
CCAAATTATA GCCTAATGCA TATAAATGTG AATAATACTG AATTACGGCT TCTATTCTCC 
AGTTCTGCAG CATTTGCAAA GTTTGAGATA ACTTTGTTTC CCTCGGCCCA TTATCCCTTG 
GTCCCACTAC CATTCACCAT TCAAAATCAC CTTGGGAAGA CTGGCCATGA TGAAATTGCT 
GCCGTTATCT CTAAAGTGCC CTTAGAGGAA AACTACCTGA AGAATGTAGT CAAGCAGATT 
TACCAAGATC TACTTAAGGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008760320.1
CDS	61..6444
Translation	MRRSARTPDKSSRKTFSKMDGIYSEANEENDNTQRPVRRQHSSILKPPRSPLQDLKCGNEIVQESNAPRTRKSSRRVSFADTIKNKNLDDNYCAITGMNTLLCAPIQTQMQQKEFSITDCNPERKHANDQTVIFSDENQMDLTASHTVMIAKGLVDCTKHENSTKIDTTSFLENLKHRAANSRVKNDLACPIISINQNIFSEKKNSDNFIKRLKMGKYNTSSTELDKENAEIPVHSKDSNSASSTCQIHASLSVDEHSSNKTRLFTEQDDGMNLTQCHTACIQTWIPPSREATLGEVKGDNTIYGNECMELTTNYTIQVLSSENNLPERENQAQNDVIDVTTVDGATAPEKKTALKDKLNAAFHSSFPNPENKLHITKCHPIESETHTVTQISNQNANTLAMTSESVCSSPAIQGYKTIFYSSSNDAMELTKCLSIMQEEKKLLKTDDKYSKICTNSDASPLREKTIYSEEDSMDITKSHTVAIDNKIFKHDQENIKKEITALPIFEKEMMLQNLKTMSKDEKMNVNSITVPQVSKESLQQSLTNALSISLADKTMEFLAGEDMDLTKSHTTKLSQVIPTTFDLASKNVTKSSSHSKSPSDEWEGLEKQIVLGQHSKLPSPQRKDLDDPDCSHHKIMYSEEVQTMDLTKSHTIVIGFGSSEVQEHSKINLEHKSSQLTAENRQTAVNIPGINFRVVTTNDMDMLKDRSIHKPELLEEKQNVKIYGRKSMGGLKIDKTILFSEGNEDDMDITKSCTVKINHRSLLDKHDSHLVSLAGTSKTILHARGQDEMEINRNHTTPLECKIISPSDITSGDLSKTVMSIDDHEELEMTKSHTVFIDYQAEAKGVLPDRLDFQLSKKKSLQKPKMTSTHAEEIVSISKNSESNYLPAKGSQVTILEEGSNSGPGEETNDAQKPGFLNEPLSGKTQRRESLKLKNKTITFSGNDKSNRDITQSSVVEINNETNLEDRKGFSFVPLAGTSKPVLSAYRPEDMEISISQTSASEYKTIPPDEMTTIPMDKTVMFVDNLSDLGVTRSHTFFIDCQAKEKVLHEYTNLGIPKPQNLSGSEDNSCIQEITKKPVAEYKHHMTTVIPSNTLFSDQSTMKIKMHKADIDEEGIGKEVEADTLKQTKLERCLLNRTDGKNVDFTSSHIADVCTSRDKYSSLPNVISSSDNLDSNTKSLCDKSREKAYNCQVPNEFTFADNLPSRYHLDSEIPSVFVSCPSKEVTQTESAIALLQDQDQEPLGLSKCASLKCAKDQMEAFVDVSVASQPHLSAQQPPSTQKGQDAANRDEGMLSKAGKKTLPFLIGNVSAPTCENASKMPTNEEHFAVTYNKELEKSIQTDKCNTNVHNHSNSVLTTQVIQTHASAEAASDSIVPSNGSCFSSAKPSLSNLNRKTGKVLDFNTVHILPPAEQLLEAGNQAHSMSIVQGTEIHSLGSSNDRDKESKTLHNEAEATSVPLKAVVKDKARRCSLGIFLPKLPNKKSCSITGVDDVEQILADATDLTHLETQPVCSQDPGIGSVAAKLNLSPSQYINEENLPVYPGEILSSDSVILDIEESAPIDTYHKEILPSENKTETCRAQKRAWVQENEVKNEKKIRTHDGAQDQEIFDHYTEEDINKSINNVFLKSLSRTPSSSSSSLDSIKADGISLDASTQRNSQMESQFLGDTICEESLREKLKDGQITIKEFFILLQVHILIQKPRQSTLPAKFTINTLPTTEDLMLRQYVYGPKIQIYREDCELLRQKIEELKVSALNQDKLLVDVNRNLWEKMKDYSDEELKTYGIYLNKIKSRYTKMTKVFTHQGKVDLYSKLVHSAETEKRKLQIKINEMDTILKKINNCLTAVEIETKNLEAEEKDDAMEDWDSEIRDAEKELERLKTEEEELQRKFLEVETQKTQTLAQIEFIKEQTTETEELLDQLSFSEWDIIEWSDEQAVFTFVYDSIELIITFGEPQVGLPFLDKAYRTINELSFQSLLDEDKAPPSSLLVHKLIFQYIEEQEFWKKKCTTQHQVPQMLQELSLVVNHCRLLGEEIEFLKRWGPNYSLMHINVNNTELRLLFSSSAAFAKFEITLFPSAHYPLVPLPFTIQNHLGKTGHDEIAAVISKVPLEENYLKNVVKQIYQDLLKD

Target ORF information:

RefSeq Version	XM_008762098.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus kinetochore scaffold 1 (Knl1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008762098.1

ORF Insert Sequence:

ATGCGGAGGA GCGCGCGGAC CCCAGACAAG TCAAGCAGAA AAACGTTTTC AAAAATGGAT 
GGGATATATT CAGAGGCTAA TGAAGAGAAT GACAATACAC AGAGGCCTGT TAGAAGACAG 
CATTCCTCAA TTTTGAAACC CCCAAGGAGT CCTCTTCAGG ACCTCAAATG TGGAAATGAA 
ATAGTTCAGG AATCTAATGC TCCGAGAACC AGGAAAAGCT CTCGTCGGGT CAGCTTTGCA 
GATACTATAA AGAACAAGAA TTTAGATGAT AACTATTGTG CAATTACTGG AATGAACACT 
TTGCTTTGTG CTCCAATTCA GACTCAGATG CAACAAAAGG AGTTTTCAAT TACAGACTGC 
AACCCTGAAA GGAAACATGC AAATGATCAA ACAGTCATCT TTTCAGATGA AAATCAGATG 
GATCTGACAG CAAGTCACAC GGTGATGATT GCCAAAGGTC TTGTAGACTG TACCAAACAT 
GAAAATTCCA CAAAAATAGA TACCACATCC TTTCTGGAGA ATTTAAAGCA TCGTGCTGCG 
AATTCAAGAG TTAAAAACGA TTTAGCATGT CCAATAATTT CTATAAATCA AAACATTTTT 
TCAGAAAAGA AAAATTCTGA CAACTTCATA AAAAGATTGA AAATGGGGAA ATATAATACT 
TCTTCTACAG AACTTGATAA GGAAAATGCT GAAATACCTG TTCATTCCAA AGACTCAAAC 
AGTGCCTCTT CCACGTGTCA AATACATGCA TCGCTTAGTG TCGATGAACA TAGTAGTAAT 
AAGACTAGAC TCTTTACAGA ACAAGATGAT GGGATGAATT TGACCCAGTG TCATACAGCC 
TGTATTCAGA CTTGGATTCC TCCATCCAGG GAGGCCACCT TGGGAGAGGT TAAAGGTGAT 
AATACAATTT ATGGCAATGA GTGTATGGAG TTGACAACTA ACTACACGAT ACAGGTGTTG 
TCATCAGAAA ATAACTTACC TGAAAGAGAA AATCAAGCTC AAAATGATGT GATTGATGTT 
ACAACAGTCG ATGGAGCTAC AGCTCCAGAG AAAAAAACAG CCCTTAAGGA TAAACTAAAT 
GCTGCTTTTC ACAGCTCTTT CCCAAACCCT GAAAATAAAC TTCATATTAC CAAATGTCAT 
CCTATAGAGT CAGAAACTCA TACAGTCACA CAGATTTCTA ATCAAAATGC CAACACATTG 
GCTATGACCT CAGAATCTGT ATGTTCTAGC CCAGCTATTC AAGGGTATAA AACAATTTTT 
TACTCTAGTA GCAATGATGC AATGGAACTG ACTAAGTGTC TATCAATTAT GCAAGAGGAA 
AAAAAATTGC TGAAGACTGA TGATAAGTAT TCTAAAATAT GTACCAATTC AGATGCTAGC 
CCACTCAGAG AGAAAACTAT TTATTCAGAA GAGGATAGCA TGGACATTAC CAAAAGTCAC 
ACAGTTGCAA TAGATAATAA GATATTTAAA CATGATCAAG AAAACATTAA AAAAGAAATA 
ACAGCTCTAC CAATATTTGA AAAAGAAATG ATGCTCCAAA ATCTTAAAAC TATGTCCAAA 
GATGAGAAAA TGAATGTGAA TTCTATTACA GTTCCTCAAG TATCTAAAGA AAGTTTACAA 
CAGAGTCTGA CAAATGCCTT ATCTATTTCA TTGGCTGATA AAACAATGGA ATTCTTAGCA 
GGTGAAGATA TGGATTTGAC TAAAAGTCAC ACAACTAAGT TAAGTCAAGT TATTCCTACA 
ACTTTTGACT TAGCATCAAA GAATGTCACC AAATCTTCCT CTCACAGCAA AAGCCCTTCA 
GATGAGTGGG AAGGCCTGGA AAAACAAATT GTGCTAGGCC AGCATTCTAA ACTTCCTTCA 
CCACAGAGGA AAGATTTAGA TGATCCTGAC TGTTCTCATC ATAAGATAAT GTATTCAGAG 
GAGGTACAAA CTATGGATCT AACCAAGAGC CACACTATTG TGATTGGGTT TGGTTCTTCT 
GAGGTACAAG AACACAGTAA GATTAATTTA GAACACAAAA GCAGCCAACT TACAGCAGAA 
AACAGACAAA CAGCTGTGAA CATTCCTGGA ATAAACTTTA GAGTAGTAAC AACTAATGAT 
ATGGACATGT TAAAGGACAG AAGTATACAT AAGCCAGAAT TGCTGGAGGA AAAGCAAAAC 
GTCAAAATTT ATGGAAGAAA AAGTATGGGT GGACTGAAGA TTGATAAAAC TATATTATTT 
TCAGAGGGTA ATGAGGATGA TATGGATATC ACTAAGAGTT GCACAGTCAA AATAAACCAT 
CGGTCTTTAT TAGATAAACA TGATTCTCAT TTAGTATCTT TGGCAGGAAC TTCTAAAACC 
ATTTTGCATG CACGTGGACA GGATGAAATG GAAATTAATA GAAATCACAC GACTCCTTTA 
GAGTGTAAAA TTATCTCACC CAGTGATATA ACTTCTGGTG ATCTGAGTAA GACTGTGATG 
TCTATAGATG ATCATGAGGA ACTAGAAATG ACTAAGTCCC ATACTGTTTT CATTGACTAC 
CAAGCAGAGG CAAAAGGTGT ACTTCCAGAC AGACTTGACT TTCAATTATC CAAAAAGAAA 
AGCCTACAGA AGCCAAAAAT GACATCTACT CATGCTGAAG AGATTGTTTC CATTTCAAAA 
AATAGTGAAA GCAACTATCT ACCAGCCAAA GGCAGCCAGG TAACAATATT AGAGGAAGGA 
TCTAATAGTG GGCCTGGGGA AGAAACTAAT GATGCACAAA AGCCTGGATT TCTGAATGAA 
CCTCTGTCAG GCAAAACTCA AAGAAGAGAA AGCCTTAAGC TCAAAAATAA GACTATTACA 
TTTTCAGGAA ATGATAAAAG TAATAGAGAT ATCACTCAGA GTTCTGTGGT AGAAATAAAT 
AATGAAACTA ACCTGGAAGA TAGAAAGGGC TTCAGTTTTG TGCCATTGGC AGGAACTTCC 
AAGCCAGTTT TATCTGCATA TCGGCCAGAG GACATGGAGA TCTCTATAAG TCAAACCTCT 
GCTTCGGAGT ATAAAACTAT CCCACCAGAT GAAATGACTA CTATACCTAT GGATAAAACT 
GTAATGTTTG TAGATAATCT CAGTGATCTG GGTGTCACCA GATCCCATAC GTTTTTCATC 
GATTGTCAAG CCAAAGAGAA AGTACTTCAT GAATATACCA ACTTGGGAAT ACCAAAACCA 
CAAAACTTGA GTGGTTCTGA AGATAATTCC TGTATTCAAG AAATCACCAA AAAACCAGTA 
GCTGAATACA AACACCATAT GACAACTGTT ATTCCTTCTA ATACATTATT TAGTGATCAA 
AGTACAATGA AAATCAAGAT GCATAAAGCT GATATAGATG AAGAGGGCAT AGGAAAGGAA 
GTGGAGGCCG ATACATTGAA ACAAACTAAA CTTGAAAGGT GTCTGTTAAA TAGAACTGAT 
GGAAAAAATG TGGATTTTAC AAGTAGTCAT ATAGCTGATG TTTGTACATC CAGAGACAAG 
TATTCCTCTT TACCAAATGT TATTTCTTCT TCTGATAATT TAGATAGTAA TACTAAGTCC 
TTGTGTGATA AAAGTAGGGA GAAAGCTTAT AATTGCCAAG TGCCAAATGA GTTTACTTTT 
GCAGATAATT TACCAAGTAG ATATCACCTG GATTCTGAAA TACCATCTGT CTTTGTATCA 
TGTCCTTCAA AAGAAGTTAC TCAAACTGAA AGTGCCATAG CACTACTTCA AGATCAAGAT 
CAAGAGCCTC TGGGACTCTC AAAGTGTGCA TCTCTTAAGT GTGCAAAGGA TCAAATGGAG 
GCCTTTGTTG ATGTTAGTGT TGCTTCTCAG CCTCATCTCT CAGCCCAACA ACCTCCATCA 
ACTCAAAAAG GGCAGGATGC CGCCAATAGA GATGAAGGAA TGCTATCTAA AGCTGGGAAG 
AAGACTTTAC CTTTTCTTAT AGGAAATGTC TCTGCACCCA CATGTGAAAA TGCGTCCAAA 
ATGCCTACTA ATGAGGAACA CTTTGCTGTA ACCTATAACA AAGAACTGGA AAAAAGTATT 
CAAACAGATA AATGTAATAC AAATGTACAC AACCATAGTA ACTCAGTCCT GACAACACAA 
GTCATTCAAA CACATGCCAG TGCCGAAGCA GCATCAGACT CTATAGTTCC ATCTAATGGT 
TCCTGTTTTA GTAGTGCCAA ACCAAGTCTG AGTAATTTGA ATAGAAAAAC TGGGAAGGTT 
TTAGACTTTA ATACTGTTCA TATATTACCT CCTGCAGAAC AGTTACTTGA AGCAGGAAAT 
CAGGCACATA GTATGAGTAT AGTACAAGGT ACAGAAATAC ATAGCCTAGG TTCCAGCAAT 
GACAGAGATA AAGAAAGTAA AACTCTGCAT AATGAGGCCG AAGCTACATC TGTACCACTA 
AAAGCAGTTG TTAAAGACAA AGCAAGAAGA TGTTCATTGG GAATATTTTT ACCCAAATTA 
CCAAACAAGA AAAGTTGTAG TATCACTGGT GTTGATGATG TGGAACAGAT TCTAGCAGAT 
GCAACTGACT TAACTCACCT AGAAACTCAG CCAGTCTGTA GTCAAGATCC GGGCATTGGC 
TCTGTTGCAG CCAAACTGAA CTTGAGTCCA TCTCAGTATA TAAATGAGGA GAATCTCCCT 
GTATATCCCG GTGAGATACT TTCATCAGAT TCTGTCATCC TGGACATTGA GGAAAGTGCT 
CCGATCGACA CATATCATAA AGAGATTTTA CCATCTGAAA ACAAAACAGA AACCTGCAGG 
GCCCAAAAAA GAGCCTGGGT TCAAGAAAAT GAGGTTAAAA ATGAGAAAAA AATCAGAACA 
CATGATGGTG CACAAGATCA AGAGATTTTT GATCATTATA CTGAAGAGGA CATCAATAAA 
AGTATTAACA ATGTATTTCT AAAAAGCCTG AGCAGGACAC CGTCTAGTTC TAGCAGCTCT 
CTGGATTCAA TCAAGGCTGA TGGAATCTCT CTGGATGCCA GCACACAGCG TAATAGCCAA 
ATGGAATCAC AGTTTCTTGG TGACACTATC TGTGAAGAGA GTTTGAGGGA GAAACTGAAG 
GATGGACAAA TTACAATCAA AGAGTTCTTC ATACTTCTTC AAGTTCATAT CTTAATACAG 
AAACCTAGGC AGAGCACTCT CCCAGCCAAA TTTACTATAA ACACATTACC TACCACAGAA 
GATCTGATGT TGAGACAATA TGTTTACGGA CCCAAGATAC AAATTTACAG AGAAGATTGT 
GAGCTTCTTC GACAGAAGAT TGAAGAATTG AAGGTATCTG CGTTGAACCA AGATAAACTA 
TTGGTGGATG TAAATAGGAA CTTGTGGGAA AAAATGAAAG ACTACTCTGA CGAAGAGCTG 
AAGACCTATG GAATTTACCT TAACAAAATA AAATCACGTT ATACCAAGAT GACGAAAGTC 
TTCACTCACC AAGGAAAAGT GGATCTGTAT AGCAAATTGG TGCACTCAGC TGAGACTGAG 
AAGAGGAAAC TTCAGATAAA AATCAACGAG ATGGACACCA TACTTAAGAA GATCAATAAC 
TGTCTCACTG CAGTGGAAAT AGAAACTAAG AATTTGGAGG CTGAAGAGAA AGATGATGCC 
ATGGAAGATT GGGATTCTGA AATACGAGAT GCAGAGAAAG AACTGGAAAG GCTGAAAACT 
GAGGAAGAGG AGCTTCAAAG AAAGTTCTTA GAAGTAGAGA CACAGAAAAC GCAGACCCTT 
GCTCAGATAG AATTTATAAA AGAACAAACA ACTGAAACAG AAGAATTATT GGATCAATTG 
AGTTTTTCTG AGTGGGATAT CATCGAGTGG AGTGATGAAC AAGCGGTATT TACCTTTGTT 
TATGATTCAA TAGAACTTAT CATCACTTTT GGAGAGCCTC AGGTTGGTCT CCCTTTCTTG 
GACAAGGCTT ACAGGACGAT TAATGAACTC AGTTTTCAGT CTCTGTTAGA CGAAGATAAA 
GCGCCTCCTT CCTCCCTCTT GGTCCACAAG CTTATTTTCC AGTACATTGA AGAGCAGGAG 
TTCTGGAAGA AGAAGTGCAC AACACAGCAT CAGGTGCCCC AGATGCTTCA AGAACTCTCA 
TTGGTAGTTA ACCATTGTAG GCTTCTTGGA GAAGAGATTG AATTTTTAAA GCGATGGGGA 
CCAAATTATA GCCTAATGCA TATAAATGTG AATAATACTG AATTACGGCT TCTATTCTCC 
AGTTCTGCAG CATTTGCAAA GTTTGAGATA ACTTTGTTTC CCTCGGCCCA TTATCCCTTG 
GTCCCACTAC CATTCACCAT TCAAAATCAC CTTGGGAAGA CTGGCCATGA TGAAATTGCT 
GCCGTTATCT CTAAAGTGCC CTTAGAGGAA AACTACCTGA AGAATGTAGT CAAGCAGATT 
TACCAAGATC TACTTAAGGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Kinetochore KMN network gene CASC5 mutated in primary microcephaly.
Human molecular genetics21(24)5306-17(2012 Dec)
Genin A,Desir J,Lambert N,Biervliet M,Van Der Aa N,Pierquin G,Killian A,Tosi M,Urbina M,Lefort A,Libert F,Pirson I,Abramowicz M

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Knl1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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