KNL1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	KNL1
Entrez Gene ID	478260
Full Name	kinetochore scaffold 1
General protein information	Preferred Names kinetochore scaffold 1 Names protein CASC5 kinetochore scaffold 1
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014109669.1	XP_013965144.1	protein CASC5 isoform X1
XM_005638255.2	XP_005638312.1	kinetochore scaffold 1 isoform X1
XM_005638255.1	XP_005638312.1	protein CASC5 isoform X1
XM_005638256.2	XP_005638313.1	kinetochore scaffold 1 isoform X2
XM_005638256.1	XP_005638313.1	protein CASC5 isoform X2
XM_014109668.1	XP_013965143.1	protein CASC5 isoform X1
XM_014109668.2	XP_013965143.1	kinetochore scaffold 1 isoform X1
XM_022412663.1	XP_022268371.1	kinetochore scaffold 1 isoform X3

Pathways from BioSystems

Homologies

Homo sapiens (human)	CASC5	NP_733468.3
Pan troglodytes (chimpanzee)	CASC5	XP_510312.3
Canis lupus familiaris (dog)	CASC5	XP_005638312.1
Bos taurus (cattle)	CASC5	XP_002690867.3
Mus musculus (house mouse)	Casc5	NP_083893.2
Rattus norvegicus (Norway rat)	Casc5	NP_001164065.1
Gallus gallus (chicken)	CASC5	XP_420938.4

Gene Ontology

The following KNL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KNL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe283631	XM_022412663.1 Latest version!	Canis lupus familiaris kinetochore scaffold 1 (KNL1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCe28661	XM_014109668.2 Latest version!	Canis lupus familiaris kinetochore scaffold 1 (KNL1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCe28661	XM_014109669.1 Latest version!	Canis lupus familiaris cancer susceptibility candidate 5 (CASC5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCe28661	XM_005638255.2 Latest version!	Canis lupus familiaris kinetochore scaffold 1 (KNL1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCe28662	XM_005638256.2 Latest version!	Canis lupus familiaris kinetochore scaffold 1 (KNL1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
View Old Accession Versions >>
OCe28661	XM_005638255.1	Canis lupus familiaris cancer susceptibility candidate 5 (CASC5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCe28662	XM_005638256.1	Canis lupus familiaris cancer susceptibility candidate 5 (CASC5), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCe28661	XM_014109668.1	Canis lupus familiaris cancer susceptibility candidate 5 (CASC5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

KNL1 ( XM_022412663.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022268371.1 Canis lupus familiaris kinetochore scaffold 1 (KNL1), transcript variant X4, mRNA.

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CloneID	OCe283631
Clone ID Related Accession (Same CDS sequence)	XM_022412663.1
Accession Version	XM_022412663.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6774bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	kinetochore scaffold 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006612.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGGGA TGTCTTCAGA GACTAATGAA GAGAATGACA ATATGGAGAG ACCTGTTAGA 
AGACGACATT CCTCCATTTT GAAACCCCCA AGGAGTCCTC TTCAGGACCT CAGAGGTGGG 
AATGAAGCAG TTCAGGAATC TAATGCTTTG AGAAATAAGA AAAGCTCTCG TCGAGTCAGC 
TTTGCAGATA CCATAAAGAA TAAGAAGCCA GAAGAAACCT ACTGTGAAAT TACTGGGATG 
AACACATTGC TTTCTGCTCC TATTCAGACT CAGATGCAAC AGAGAGAGAT TTCAATTACA 
GAACACAACC ATGAAATGAA ACCTGCAAAT GACCAAACAG TCATCTTTTC AGATGAAAAC 
CAGATGGACC TAACAGCAAG TCACACCGTA ATGATTACCA AAGACCTGTT AGATTGTACT 
AAAAGTGAAA AGTCCATTAA GATAGATACC GCATCATTTC TGGCTAACTT AAAGCTCCAT 
ACTAAGGATT CAAGAATGAA AAAAGAATTA AATTTTTCCA TGGATCAAAG CACTTCTTCA 
GAAAAGAAAA TACATTTTAA TGACTTCATA AAAAGATTGA AAACAGGAAA ATCTAATGCT 
TTTCCTACAG GACCTGATAA AGAAAATTTT GAAATATCTG TTCATCCCAT AAAAACAAAG 
AAGGCCTCTT CTGTACATCA AATGCATGTA TCTCTTAGGG TAGATGAAAA TAGTAGTAAT 
ACAACTAGAA TCTTCGGAGA ACAAAATGAT GGGATGAATT TCACTCAGTG TCATACAGCC 
AGTATTCAGA CTTTGGTTCC CACATCCAGT AAGGCCAGCA TAGGGGAATT TAAAGGTGAT 
GATATTACAA TTTATGGCAG TGATTTTATG GACTTGACAA CTAACCACAC TATACAGAAT 
TTACCCTCAG CAGATAATTC ATCTAATAAA GAAAATCAAA CTCAGAATGT CATGATGGAG 
GTTAAAGCTC CAGGAGGGAA GACAATCTTT AATAAACTGA ATATTCCTTT TCAAACCCCT 
TTAAACCCTA ATGGTGAAAT ACATATTACC AAAAGTCATA TTACAGGGAC AGAAACTCAC 
ACAAGCACAC AGACTTCTAA CCAAGATGCC AGAACATTGA CTATAATCCC AGAATCTATA 
TGTTCCAGTC CAGCTACTCA AGATTATAAG ACATTTTTCT ATTCTAGCAG TAATAATGCC 
ATGGAACTGA CCACGTGTCT CTCGGGTATG AGAAAAGAGA AGAATTTGCT GAAGAATGAT 
AATTATTCTA AAATGTATCC CAATCCAGAT GCCATCTCTC TTCTTACAGA GAAAACTATT 
TATTCAGGAG AGGATAGCAT GGACATTACG AGGAGTCATA CAGTTGCAAT AGATAATCAG 
ATTTTTAAAC AAGATCAGAC AAATATACAG AAAGCAGACA CATCAATATC TGAAAAAGAA 
ATAATGCTCC AAAATCTCAT AACCATGTCA AAGGACGGGA AAATGAATAT AAATTGTAGC 
TCAGTTCCTC ATATATCTAA GGGAAGATTA CAGCAGAGCC TGGCAAACTC TTTATCTATT 
TCATTGACTG ACAAAAAGAC TGAAATCTTC ACAGGTGAAG ATATGGATTT GACTAAAAGT 
CACACAACTA ACTTAGGAAG TCAAGCTCCA CTTACATCTT ACAATGTAAC ATCTGAGAAT 
ACCAGTAAAT TTCTCTCTCA CAGCAAAAGC CCTTCAGGTG AATGGCAAGA AATGACCAAA 
AGTCGTATTG AACCATCCCA GCAGCCAGAC ATAATATCTA AAAACATCCC AGTGGACACC 
TGGGGCAAAG AAAAAGTTCA AGTTTTAGAG ATTGCACCTT ATGTTGATAA AGATTCTCCT 
CAATCAGCTG ATATTAATCA AGACATTGCA ATAAGCCATA GTATAGTATA CTCTGATAGA 
AATCTTGAGA AACAAGTAGC ACTAGGAAAT AATAGAAATA CTGTTCCATG TGAGCAATCT 
TTGTTTCCTA CTTCAGAGTC ATTATTTTCA TTAGAAGGAC AGTCTAACAT GAAAAATCAT 
AATATTGCTA TTAACAGTTA CACAATGAAA TCTGTGCCAG GCCAGAATTC TAAACTGCCT 
GAGCCACTGA GGAAAGGTGT AGGCAATCCA ACACCTGACT ATTCTCATGA CAAAACAATT 
ATTTGTTCAG AGGAGGAACA AAATATGGAT CTAACCAAGA GTCACACTGT TGTCATTGGA 
TTTAGTCCTT CTGAAATACA AGAATTGGGT AAGACTAATC TAGAAAATAC TAACAGTCTG 
CTAACAACAG CAAACAGACA GACAGCTATA AAAGTTGAAA AATGTTGTAA AAGTCCTATA 
GAAAAAACAG GAATATCTGT TTCTAAGGAT AACCTAGATG TGATAGAGGA CAATAGCATA 
CAGAAACTTG GATTTTTGAA TGAAAAGCAA GATGTCAAAA TTTGTGGAAG AAAAAGTGTT 
GGCAGACAAA AAGTTGATAA GACTATTGTA TTTTCAGAGG GTGATGAGAA TGATATGGAT 
ATCACCAAGA TTTTTACAGT GGAAATAAAT CATAAACCTT TATTACTTGA ACAAGATTCC 
CATTTGGCAG GAACTTCTAA AACTGTTTTG TATGAATGTG GGCAAGATGA TGTGGAGATC 
ACTAAAAGTC ACACAACTGC CTTGGAATGT ATAACTGTCT CACCAGATAA AATAACCAGT 
AGGCCTATGG ACAAAACTGT AATGTTTGTA GATAATTACA ATGAACTGGA AATGACAAAG 
TCTCACACTG TTTTCATTGA CTACCAAGCA AAGGAGAGGA CTGTGAGTAT AGACAAACTT 
GACACGGAAC TATCCAAAAG AAGAAAAAGC ATAGAAAAGC CAAAAATGAC ACCTGCTTCT 
GCTGAGGAGA GTGTTTCCTT TCTGGAGAAT GGTGAAAGTG ACCATTCAGC AGCAAACGTC 
AATAAGCTAA CACTACTGAG GGAATGGTCT AATAATGGTC CTATAGAGAA AGCAGTAGCT 
GGTGATAACA TGGAAGTATC TAAATTCACC ATTTGGAAAA ATGGCAAAGA TGTACAAAAG 
CCTGAATTTC TGAATGACAC TATATCAGAC AAAAGTCAGA GAAGGAAAAG CCTTAGACTC 
AAAAATGACA AGATTGCTGC ATTTTCTAAA AATGATGATG ATATGGATAT CACCCAGAGT 
TGTACAGTGA AAATAAACTA TAAAAGTGCC CCAGAAGATA GAGAGGATTC CCATTTGGTG 
CCTTTGACAG GAGCTTCTAA AACTGTTTTG TATTCATGTG GGCAGGATGA CATGGAGATG 
ACTAGAAGTC ATACGACTGC GTTAGAATGT AAAACTGTCT CACCGGATAA AATCACTACT 
AGGCCCATAG ATAAAACTGT AATGTTTGTT GATAATCATG GTGATCTGGA AGTCACCGAG 
TCCCATACTG TTTTCATTGA TTGTCAAAAC ACAGAGAAAA TTAATCAAGA GTGCCCTAAA 
TTTGGAATAG CTACAGGAAA ACCCTTGGGT ATTTCTTTTC ATAATGATGG TAGCTCTGTT 
CAAGAAATCA CTGAAAAACA AGCATTGGCA GTAGACCAGA CCTATATACC GGAAAAAGCC 
AGAATTGGAA GCTGTCAGTT AAATAGTACA GATAGAAGAA ACATGGACTT TACTAACAGT 
CAAGCAACTG CTACGTGTGG ATCCAGTGAT AATTATTCCT GTTTACCAAA TGCTATTTCC 
TGTTTTGATA ATTTAGAGGG GAATGTCATG TCCTTAAGTA ATAAAGATAA GAAAGCCAGT 
AACTGCCCAG TGCAAAATGA TCTTTCTTAT GTAAAGGATT TAGCCAATGA ATATTACTTG 
AAACCTGAGG GACTGCCTCC CTCTGCTTGT TCTTTGTTAA AGAAAGAAAA AGTGACTCAA 
ACCAATACCA ATGGACAGTT AGACTGTGTT ACAACAGTGC TTAAAGATCA AGATATGATT 
AAGGAGTCCC AGAATCTAAT AGCTAATCAA ACTTTACAAG ATAGTCAGGA GCTGGCGGAG 
ATGATTAAAC CTAATTCAAA TCGAGTATCC TTTAAACCCC CAAAGGATCA AATGGAGGGC 
TTTGTTGATA CCACGGAACA TAATTTAAAG AAGACTGTTG TTGATAATGT TTGTGTTACT 
TCTCAGCCTC ATCTCACAAG CCAGGTACCT CCATTACCTC AACAAGGACG GATAATTTTG 
AATAAAGATG AAACAATATC ATCTAATTCT GGAAATAAGA ATTTAAATAT TGTTATAGGA 
AATTCCTTTG TACCCACATG TGAAAACGAG GCCAAAATGC TCAACAATGA TAAACAATTT 
ACCATAGTCT GTAAAAATGA GCTGAGGAAA AATACTAAAT GTAATACCGA TTTCCACAGT 
AATTCAGACT TGACTAAGCA AGTTATTCAA ACTCATACTA ATTCTAGAGA AGCATCAGAT 
ACTGTAATTG CATCTAATAC AAGTTTTAAT AGTGTCAGAT CAAATCTGAA TAATTTGAAT 
GGAAAAACTG AAGAATTTTT AGATTTCCAA ACTCCCCATA TTCCATCTTC TCCAGAGCAA 
ATACTTGCAT TAATAAACAA GGCACACAAT CATATGAGTA TATTGCAAGC TACAGAAATA 
CAGAACATTA ACCCAGCGCC CAGCAATGTT GAAGATGATA AAGATGAAGA AAGCAAAATT 
TCTCATAATG GAGCTGAAAC CAACTCTGTA CCACTCATGA CAGTTGTAAA AGATAAAGTG 
AAGAGATGTT CTTTGGGAAT ATTTTTGCCC AGATTGCCAA ACAAGAGAAA TTGTAGTGTC 
ACTGGTATTA ATGACCTGAA GCAGATTCAA GGAGACACAA CTGATTTAAA TCACTTAGAA 
ATTCAGCCAG TCTTCAGTAA GGAGCCAAGC ATTGAATTTG TTGCAACTAA ACTGACCTTA 
AGTCCATCTC AGTATATAAA TGAGGAAAAC TTTCCTATAT ATCCTGGTGA GATTAATTCC 
TCAGATTCTA TTACCATAGA AACTGAGGAA AAGGCTTTGA TTGAGACATA TCAAAAAGGG 
ATTCCACCAT CTGAAAATAA GATAAGAGAA ACCTGCAGTA ACCAAAAAAG AACCTGGGTA 
CAAGAAGATG ATGATATTCA GAATGAGAAA AAAATCAGGA AAAGTGAGAT TAGGTGTAGT 
GATACTACAC AAGATCAGGA GATTTTTGAT CACCATGCTG AAGGGGATAT AGATAAAAAT 
GCTAACAGCC TGAGCAGAAC CCCATCTAGT TGCAGCAGTT CTCTGGATTC GATAAAGGCT 
GATGGGACCT CTCTGGACTT CAGCACACAG CACAATAGTC AAATGGAATC ACAGTTTCTC 
AGAGACACTA TTTGTGAAGA GAGCTTGAAG GAGAAACTCA AAGATGGGAA AATTACAATA 
AAGGAGTTCT TTATACTTCT TCAGGTCCAC ATTTTGATAC AGAAACCCCG ACAGAGCAAT 
CTCCCAGCCA AATTTACTAT AAACACACCA CCTACTCTGG AAGACCTGAT GTTAAGTCAG 
TATGTTTACC GACCCAAGAT ACAGATTTAT AGAGAAGATT GTGAAGCTCT TTGCCAAAAG 
ATTGAAGAAT TAAAGCTTTC TGCATTGAAC CAGGATAAAT TGTTGACTGA CATAAATAGG 
AACCTGTGGG AAAAAATGAG ACACTGCTCT GATGAAGAGC TGAAGGTCTT TGGAATTTAC 
CTGAACAAAA TAAAATCACA TTTTACCAAG ATGACTAAAG TTTTTACTCA CCAAGGAAAA 
GTGGCTCTCT ATACCAAGCT GGTACAGTCA GCTCAGAATG AGAGGAAGAA ACTTCAGATA 
AGGATAGATG AGATGGACAA CATACTTAAG AAGATTGAGA ACTGTCTCGC TGAAGTTGAA 
ATAGAAACTA AGAATTTGGA TGAAGAGAAA GACAATCCTA TGGAAGAATG GGATTCTGAA 
ATGAGAGCCG CAGAGAAAGA ACTAGAACAG CTGAAAACTG AAGAAGATGA GCTCCAAAGA 
AATCTCTTAG AACTGGAAGT ACAGAAAGAG CAGACCCTTG CTCAAATAGA CTTTGTACAA 
AAACAAACAG ATAGAACTGA AGAGCTAATG GATCAGTTGA GCTTGTCTGA GTGGGAAGCC 
ATTGAGTGGA GTGATGATCA AGCTGTATTC ACCTTTCTTT ATGACACAAT AGAACTTACT 
ATCACCTTTG GAGAGACAGT AGCTGGAAGT CCCAAGATCA GGCTGCCAGC ATGGTCAGTT 
GATCTCCCTT TCCTGGACAA GGCTTGTAGG AAGATTGTTG ACTTGAATTT TCAATCTCGG 
TTAGATGAGG ATAAAGCTCC TCCTTCCTCC CTTTTAGTTC ATAAGCTTAT TTTCCAGTAC 
ATTGAGGAAA AGGAATCCTG GAAGAAAAAA TGTACAACAC AGCATCAAAT ACCCAAGATG 
CTTCAAGAAA TCTCATTGGT AGTGAGCCAT TGTAGACTCC TTGGAGAGGA AATTGAGTTT 
TTAAAGAGAT GGGGACCAAA TTATAACCTA ATGAACATAG ATGTGACTAA TACTGAATTG 
AGGCTTTTAT TCTCCAGCTC TGCAGCATTT GCAAAATTTG AGATAACTTT GCCTCTCTCA 
GTCCATTTTC CAACTGTTCC ATTACCTTTC TCCATTCAAA AGCACCTTGG AAACATTGGC 
CAAGATGAAA TCACTGCAAT TCTATCTAAA GTGCCACTGA AGAACAACTA TCTAAAGAAT 
GTAGTCAAGC AAATTTACCA AGATCTGCTT CAGGACTGCC ATTTCTATCA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022268371.1
CDS	339..7112
Translation	MDGMSSETNEENDNMERPVRRRHSSILKPPRSPLQDLRGGNEAVQESNALRNKKSSRRVSFADTIKNKKPEETYCEITGMNTLLSAPIQTQMQQREISITEHNHEMKPANDQTVIFSDENQMDLTASHTVMITKDLLDCTKSEKSIKIDTASFLANLKLHTKDSRMKKELNFSMDQSTSSEKKIHFNDFIKRLKTGKSNAFPTGPDKENFEISVHPIKTKKASSVHQMHVSLRVDENSSNTTRIFGEQNDGMNFTQCHTASIQTLVPTSSKASIGEFKGDDITIYGSDFMDLTTNHTIQNLPSADNSSNKENQTQNVMMEVKAPGGKTIFNKLNIPFQTPLNPNGEIHITKSHITGTETHTSTQTSNQDARTLTIIPESICSSPATQDYKTFFYSSSNNAMELTTCLSGMRKEKNLLKNDNYSKMYPNPDAISLLTEKTIYSGEDSMDITRSHTVAIDNQIFKQDQTNIQKADTSISEKEIMLQNLITMSKDGKMNINCSSVPHISKGRLQQSLANSLSISLTDKKTEIFTGEDMDLTKSHTTNLGSQAPLTSYNVTSENTSKFLSHSKSPSGEWQEMTKSRIEPSQQPDIISKNIPVDTWGKEKVQVLEIAPYVDKDSPQSADINQDIAISHSIVYSDRNLEKQVALGNNRNTVPCEQSLFPTSESLFSLEGQSNMKNHNIAINSYTMKSVPGQNSKLPEPLRKGVGNPTPDYSHDKTIICSEEEQNMDLTKSHTVVIGFSPSEIQELGKTNLENTNSLLTTANRQTAIKVEKCCKSPIEKTGISVSKDNLDVIEDNSIQKLGFLNEKQDVKICGRKSVGRQKVDKTIVFSEGDENDMDITKIFTVEINHKPLLLEQDSHLAGTSKTVLYECGQDDVEITKSHTTALECITVSPDKITSRPMDKTVMFVDNYNELEMTKSHTVFIDYQAKERTVSIDKLDTELSKRRKSIEKPKMTPASAEESVSFLENGESDHSAANVNKLTLLREWSNNGPIEKAVAGDNMEVSKFTIWKNGKDVQKPEFLNDTISDKSQRRKSLRLKNDKIAAFSKNDDDMDITQSCTVKINYKSAPEDREDSHLVPLTGASKTVLYSCGQDDMEMTRSHTTALECKTVSPDKITTRPIDKTVMFVDNHGDLEVTESHTVFIDCQNTEKINQECPKFGIATGKPLGISFHNDGSSVQEITEKQALAVDQTYIPEKARIGSCQLNSTDRRNMDFTNSQATATCGSSDNYSCLPNAISCFDNLEGNVMSLSNKDKKASNCPVQNDLSYVKDLANEYYLKPEGLPPSACSLLKKEKVTQTNTNGQLDCVTTVLKDQDMIKESQNLIANQTLQDSQELAEMIKPNSNRVSFKPPKDQMEGFVDTTEHNLKKTVVDNVCVTSQPHLTSQVPPLPQQGRIILNKDETISSNSGNKNLNIVIGNSFVPTCENEAKMLNNDKQFTIVCKNELRKNTKCNTDFHSNSDLTKQVIQTHTNSREASDTVIASNTSFNSVRSNLNNLNGKTEEFLDFQTPHIPSSPEQILALINKAHNHMSILQATEIQNINPAPSNVEDDKDEESKISHNGAETNSVPLMTVVKDKVKRCSLGIFLPRLPNKRNCSVTGINDLKQIQGDTTDLNHLEIQPVFSKEPSIEFVATKLTLSPSQYINEENFPIYPGEINSSDSITIETEEKALIETYQKGIPPSENKIRETCSNQKRTWVQEDDDIQNEKKIRKSEIRCSDTTQDQEIFDHHAEGDIDKNANSLSRTPSSCSSSLDSIKADGTSLDFSTQHNSQMESQFLRDTICEESLKEKLKDGKITIKEFFILLQVHILIQKPRQSNLPAKFTINTPPTLEDLMLSQYVYRPKIQIYREDCEALCQKIEELKLSALNQDKLLTDINRNLWEKMRHCSDEELKVFGIYLNKIKSHFTKMTKVFTHQGKVALYTKLVQSAQNERKKLQIRIDEMDNILKKIENCLAEVEIETKNLDEEKDNPMEEWDSEMRAAEKELEQLKTEEDELQRNLLELEVQKEQTLAQIDFVQKQTDRTEELMDQLSLSEWEAIEWSDDQAVFTFLYDTIELTITFGETVAGSPKIRLPAWSVDLPFLDKACRKIVDLNFQSRLDEDKAPPSSLLVHKLIFQYIEEKESWKKKCTTQHQIPKMLQEISLVVSHCRLLGEEIEFLKRWGPNYNLMNIDVTNTELRLLFSSSAAFAKFEITLPLSVHFPTVPLPFSIQKHLGNIGQDEITAILSKVPLKNNYLKNVVKQIYQDLLQDCHFYH

Target ORF information:

RefSeq Version	XM_022412663.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris kinetochore scaffold 1 (KNL1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022412663.1

ORF Insert Sequence:

ATGGATGGGA TGTCTTCAGA GACTAATGAA GAGAATGACA ATATGGAGAG ACCTGTTAGA 
AGACGACATT CCTCCATTTT GAAACCCCCA AGGAGTCCTC TTCAGGACCT CAGAGGTGGG 
AATGAAGCAG TTCAGGAATC TAATGCTTTG AGAAATAAGA AAAGCTCTCG TCGAGTCAGC 
TTTGCAGATA CCATAAAGAA TAAGAAGCCA GAAGAAACCT ACTGTGAAAT TACTGGGATG 
AACACATTGC TTTCTGCTCC TATTCAGACT CAGATGCAAC AGAGAGAGAT TTCAATTACA 
GAACACAACC ATGAAATGAA ACCTGCAAAT GACCAAACAG TCATCTTTTC AGATGAAAAC 
CAGATGGACC TAACAGCAAG TCACACCGTA ATGATTACCA AAGACCTGTT AGATTGTACT 
AAAAGTGAAA AGTCCATTAA GATAGATACC GCATCATTTC TGGCTAACTT AAAGCTCCAT 
ACTAAGGATT CAAGAATGAA AAAAGAATTA AATTTTTCCA TGGATCAAAG CACTTCTTCA 
GAAAAGAAAA TACATTTTAA TGACTTCATA AAAAGATTGA AAACAGGAAA ATCTAATGCT 
TTTCCTACAG GACCTGATAA AGAAAATTTT GAAATATCTG TTCATCCCAT AAAAACAAAG 
AAGGCCTCTT CTGTACATCA AATGCATGTA TCTCTTAGGG TAGATGAAAA TAGTAGTAAT 
ACAACTAGAA TCTTCGGAGA ACAAAATGAT GGGATGAATT TCACTCAGTG TCATACAGCC 
AGTATTCAGA CTTTGGTTCC CACATCCAGT AAGGCCAGCA TAGGGGAATT TAAAGGTGAT 
GATATTACAA TTTATGGCAG TGATTTTATG GACTTGACAA CTAACCACAC TATACAGAAT 
TTACCCTCAG CAGATAATTC ATCTAATAAA GAAAATCAAA CTCAGAATGT CATGATGGAG 
GTTAAAGCTC CAGGAGGGAA GACAATCTTT AATAAACTGA ATATTCCTTT TCAAACCCCT 
TTAAACCCTA ATGGTGAAAT ACATATTACC AAAAGTCATA TTACAGGGAC AGAAACTCAC 
ACAAGCACAC AGACTTCTAA CCAAGATGCC AGAACATTGA CTATAATCCC AGAATCTATA 
TGTTCCAGTC CAGCTACTCA AGATTATAAG ACATTTTTCT ATTCTAGCAG TAATAATGCC 
ATGGAACTGA CCACGTGTCT CTCGGGTATG AGAAAAGAGA AGAATTTGCT GAAGAATGAT 
AATTATTCTA AAATGTATCC CAATCCAGAT GCCATCTCTC TTCTTACAGA GAAAACTATT 
TATTCAGGAG AGGATAGCAT GGACATTACG AGGAGTCATA CAGTTGCAAT AGATAATCAG 
ATTTTTAAAC AAGATCAGAC AAATATACAG AAAGCAGACA CATCAATATC TGAAAAAGAA 
ATAATGCTCC AAAATCTCAT AACCATGTCA AAGGACGGGA AAATGAATAT AAATTGTAGC 
TCAGTTCCTC ATATATCTAA GGGAAGATTA CAGCAGAGCC TGGCAAACTC TTTATCTATT 
TCATTGACTG ACAAAAAGAC TGAAATCTTC ACAGGTGAAG ATATGGATTT GACTAAAAGT 
CACACAACTA ACTTAGGAAG TCAAGCTCCA CTTACATCTT ACAATGTAAC ATCTGAGAAT 
ACCAGTAAAT TTCTCTCTCA CAGCAAAAGC CCTTCAGGTG AATGGCAAGA AATGACCAAA 
AGTCGTATTG AACCATCCCA GCAGCCAGAC ATAATATCTA AAAACATCCC AGTGGACACC 
TGGGGCAAAG AAAAAGTTCA AGTTTTAGAG ATTGCACCTT ATGTTGATAA AGATTCTCCT 
CAATCAGCTG ATATTAATCA AGACATTGCA ATAAGCCATA GTATAGTATA CTCTGATAGA 
AATCTTGAGA AACAAGTAGC ACTAGGAAAT AATAGAAATA CTGTTCCATG TGAGCAATCT 
TTGTTTCCTA CTTCAGAGTC ATTATTTTCA TTAGAAGGAC AGTCTAACAT GAAAAATCAT 
AATATTGCTA TTAACAGTTA CACAATGAAA TCTGTGCCAG GCCAGAATTC TAAACTGCCT 
GAGCCACTGA GGAAAGGTGT AGGCAATCCA ACACCTGACT ATTCTCATGA CAAAACAATT 
ATTTGTTCAG AGGAGGAACA AAATATGGAT CTAACCAAGA GTCACACTGT TGTCATTGGA 
TTTAGTCCTT CTGAAATACA AGAATTGGGT AAGACTAATC TAGAAAATAC TAACAGTCTG 
CTAACAACAG CAAACAGACA GACAGCTATA AAAGTTGAAA AATGTTGTAA AAGTCCTATA 
GAAAAAACAG GAATATCTGT TTCTAAGGAT AACCTAGATG TGATAGAGGA CAATAGCATA 
CAGAAACTTG GATTTTTGAA TGAAAAGCAA GATGTCAAAA TTTGTGGAAG AAAAAGTGTT 
GGCAGACAAA AAGTTGATAA GACTATTGTA TTTTCAGAGG GTGATGAGAA TGATATGGAT 
ATCACCAAGA TTTTTACAGT GGAAATAAAT CATAAACCTT TATTACTTGA ACAAGATTCC 
CATTTGGCAG GAACTTCTAA AACTGTTTTG TATGAATGTG GGCAAGATGA TGTGGAGATC 
ACTAAAAGTC ACACAACTGC CTTGGAATGT ATAACTGTCT CACCAGATAA AATAACCAGT 
AGGCCTATGG ACAAAACTGT AATGTTTGTA GATAATTACA ATGAACTGGA AATGACAAAG 
TCTCACACTG TTTTCATTGA CTACCAAGCA AAGGAGAGGA CTGTGAGTAT AGACAAACTT 
GACACGGAAC TATCCAAAAG AAGAAAAAGC ATAGAAAAGC CAAAAATGAC ACCTGCTTCT 
GCTGAGGAGA GTGTTTCCTT TCTGGAGAAT GGTGAAAGTG ACCATTCAGC AGCAAACGTC 
AATAAGCTAA CACTACTGAG GGAATGGTCT AATAATGGTC CTATAGAGAA AGCAGTAGCT 
GGTGATAACA TGGAAGTATC TAAATTCACC ATTTGGAAAA ATGGCAAAGA TGTACAAAAG 
CCTGAATTTC TGAATGACAC TATATCAGAC AAAAGTCAGA GAAGGAAAAG CCTTAGACTC 
AAAAATGACA AGATTGCTGC ATTTTCTAAA AATGATGATG ATATGGATAT CACCCAGAGT 
TGTACAGTGA AAATAAACTA TAAAAGTGCC CCAGAAGATA GAGAGGATTC CCATTTGGTG 
CCTTTGACAG GAGCTTCTAA AACTGTTTTG TATTCATGTG GGCAGGATGA CATGGAGATG 
ACTAGAAGTC ATACGACTGC GTTAGAATGT AAAACTGTCT CACCGGATAA AATCACTACT 
AGGCCCATAG ATAAAACTGT AATGTTTGTT GATAATCATG GTGATCTGGA AGTCACCGAG 
TCCCATACTG TTTTCATTGA TTGTCAAAAC ACAGAGAAAA TTAATCAAGA GTGCCCTAAA 
TTTGGAATAG CTACAGGAAA ACCCTTGGGT ATTTCTTTTC ATAATGATGG TAGCTCTGTT 
CAAGAAATCA CTGAAAAACA AGCATTGGCA GTAGACCAGA CCTATATACC GGAAAAAGCC 
AGAATTGGAA GCTGTCAGTT AAATAGTACA GATAGAAGAA ACATGGACTT TACTAACAGT 
CAAGCAACTG CTACGTGTGG ATCCAGTGAT AATTATTCCT GTTTACCAAA TGCTATTTCC 
TGTTTTGATA ATTTAGAGGG GAATGTCATG TCCTTAAGTA ATAAAGATAA GAAAGCCAGT 
AACTGCCCAG TGCAAAATGA TCTTTCTTAT GTAAAGGATT TAGCCAATGA ATATTACTTG 
AAACCTGAGG GACTGCCTCC CTCTGCTTGT TCTTTGTTAA AGAAAGAAAA AGTGACTCAA 
ACCAATACCA ATGGACAGTT AGACTGTGTT ACAACAGTGC TTAAAGATCA AGATATGATT 
AAGGAGTCCC AGAATCTAAT AGCTAATCAA ACTTTACAAG ATAGTCAGGA GCTGGCGGAG 
ATGATTAAAC CTAATTCAAA TCGAGTATCC TTTAAACCCC CAAAGGATCA AATGGAGGGC 
TTTGTTGATA CCACGGAACA TAATTTAAAG AAGACTGTTG TTGATAATGT TTGTGTTACT 
TCTCAGCCTC ATCTCACAAG CCAGGTACCT CCATTACCTC AACAAGGACG GATAATTTTG 
AATAAAGATG AAACAATATC ATCTAATTCT GGAAATAAGA ATTTAAATAT TGTTATAGGA 
AATTCCTTTG TACCCACATG TGAAAACGAG GCCAAAATGC TCAACAATGA TAAACAATTT 
ACCATAGTCT GTAAAAATGA GCTGAGGAAA AATACTAAAT GTAATACCGA TTTCCACAGT 
AATTCAGACT TGACTAAGCA AGTTATTCAA ACTCATACTA ATTCTAGAGA AGCATCAGAT 
ACTGTAATTG CATCTAATAC AAGTTTTAAT AGTGTCAGAT CAAATCTGAA TAATTTGAAT 
GGAAAAACTG AAGAATTTTT AGATTTCCAA ACTCCCCATA TTCCATCTTC TCCAGAGCAA 
ATACTTGCAT TAATAAACAA GGCACACAAT CATATGAGTA TATTGCAAGC TACAGAAATA 
CAGAACATTA ACCCAGCGCC CAGCAATGTT GAAGATGATA AAGATGAAGA AAGCAAAATT 
TCTCATAATG GAGCTGAAAC CAACTCTGTA CCACTCATGA CAGTTGTAAA AGATAAAGTG 
AAGAGATGTT CTTTGGGAAT ATTTTTGCCC AGATTGCCAA ACAAGAGAAA TTGTAGTGTC 
ACTGGTATTA ATGACCTGAA GCAGATTCAA GGAGACACAA CTGATTTAAA TCACTTAGAA 
ATTCAGCCAG TCTTCAGTAA GGAGCCAAGC ATTGAATTTG TTGCAACTAA ACTGACCTTA 
AGTCCATCTC AGTATATAAA TGAGGAAAAC TTTCCTATAT ATCCTGGTGA GATTAATTCC 
TCAGATTCTA TTACCATAGA AACTGAGGAA AAGGCTTTGA TTGAGACATA TCAAAAAGGG 
ATTCCACCAT CTGAAAATAA GATAAGAGAA ACCTGCAGTA ACCAAAAAAG AACCTGGGTA 
CAAGAAGATG ATGATATTCA GAATGAGAAA AAAATCAGGA AAAGTGAGAT TAGGTGTAGT 
GATACTACAC AAGATCAGGA GATTTTTGAT CACCATGCTG AAGGGGATAT AGATAAAAAT 
GCTAACAGCC TGAGCAGAAC CCCATCTAGT TGCAGCAGTT CTCTGGATTC GATAAAGGCT 
GATGGGACCT CTCTGGACTT CAGCACACAG CACAATAGTC AAATGGAATC ACAGTTTCTC 
AGAGACACTA TTTGTGAAGA GAGCTTGAAG GAGAAACTCA AAGATGGGAA AATTACAATA 
AAGGAGTTCT TTATACTTCT TCAGGTCCAC ATTTTGATAC AGAAACCCCG ACAGAGCAAT 
CTCCCAGCCA AATTTACTAT AAACACACCA CCTACTCTGG AAGACCTGAT GTTAAGTCAG 
TATGTTTACC GACCCAAGAT ACAGATTTAT AGAGAAGATT GTGAAGCTCT TTGCCAAAAG 
ATTGAAGAAT TAAAGCTTTC TGCATTGAAC CAGGATAAAT TGTTGACTGA CATAAATAGG 
AACCTGTGGG AAAAAATGAG ACACTGCTCT GATGAAGAGC TGAAGGTCTT TGGAATTTAC 
CTGAACAAAA TAAAATCACA TTTTACCAAG ATGACTAAAG TTTTTACTCA CCAAGGAAAA 
GTGGCTCTCT ATACCAAGCT GGTACAGTCA GCTCAGAATG AGAGGAAGAA ACTTCAGATA 
AGGATAGATG AGATGGACAA CATACTTAAG AAGATTGAGA ACTGTCTCGC TGAAGTTGAA 
ATAGAAACTA AGAATTTGGA TGAAGAGAAA GACAATCCTA TGGAAGAATG GGATTCTGAA 
ATGAGAGCCG CAGAGAAAGA ACTAGAACAG CTGAAAACTG AAGAAGATGA GCTCCAAAGA 
AATCTCTTAG AACTGGAAGT ACAGAAAGAG CAGACCCTTG CTCAAATAGA CTTTGTACAA 
AAACAAACAG ATAGAACTGA AGAGCTAATG GATCAGTTGA GCTTGTCTGA GTGGGAAGCC 
ATTGAGTGGA GTGATGATCA AGCTGTATTC ACCTTTCTTT ATGACACAAT AGAACTTACT 
ATCACCTTTG GAGAGACAGT AGCTGGAAGT CCCAAGATCA GGCTGCCAGC ATGGTCAGTT 
GATCTCCCTT TCCTGGACAA GGCTTGTAGG AAGATTGTTG ACTTGAATTT TCAATCTCGG 
TTAGATGAGG ATAAAGCTCC TCCTTCCTCC CTTTTAGTTC ATAAGCTTAT TTTCCAGTAC 
ATTGAGGAAA AGGAATCCTG GAAGAAAAAA TGTACAACAC AGCATCAAAT ACCCAAGATG 
CTTCAAGAAA TCTCATTGGT AGTGAGCCAT TGTAGACTCC TTGGAGAGGA AATTGAGTTT 
TTAAAGAGAT GGGGACCAAA TTATAACCTA ATGAACATAG ATGTGACTAA TACTGAATTG 
AGGCTTTTAT TCTCCAGCTC TGCAGCATTT GCAAAATTTG AGATAACTTT GCCTCTCTCA 
GTCCATTTTC CAACTGTTCC ATTACCTTTC TCCATTCAAA AGCACCTTGG AAACATTGGC 
CAAGATGAAA TCACTGCAAT TCTATCTAAA GTGCCACTGA AGAACAACTA TCTAAAGAAT 
GTAGTCAAGC AAATTTACCA AGATCTGCTT CAGGACTGCC ATTTCTATCA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KNL1 ( XM_014109669.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013965144.1 Canis lupus familiaris cancer susceptibility candidate 5 (CASC5), transcript variant X3, mRNA.

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CloneID	OCe28661
Clone ID Related Accession (Same CDS sequence)	XM_014109669.1 , XM_005638255.2 , XM_005638255.1 , XM_014109668.1 , XM_014109668.2
Accession Version	XM_014109669.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6861bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	protein CASC5 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726113.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGGGA TGTCTTCAGA GACTAATGAA GAGAATGACA ATATGGAGAG ACCTGTTAGA 
AGACGACATT CCTCCATTTT GAAACCCCCA AGGAGTCCTC TTCAGGACCT CAGAGGTGGG 
AATGAAGCAG TTCAGGAATC TAATGCTTTG AGAAATAAGA AAAGCTCTCG TCGAGTCAGC 
TTTGCAGATA CCATAAAAGT ATTCCAAACA GAGTCTCATA TGAAAATAGT AAGAAAGTCA 
GAAATTGCAG AAACAGAAGC AGGAGAAAAT GTTCTATATA TACAGAATAA GAAGCCAGAA 
GAAACCTACT GTGAAATTAC TGGGATGAAC ACATTGCTTT CTGCTCCTAT TCAGACTCAG 
ATGCAACAGA GAGAGATTTC AATTACAGAA CACAACCATG AAATGAAACC TGCAAATGAC 
CAAACAGTCA TCTTTTCAGA TGAAAACCAG ATGGACCTAA CAGCAAGTCA CACCGTAATG 
ATTACCAAAG ACCTGTTAGA TTGTACTAAA AGTGAAAAGT CCATTAAGAT AGATACCGCA 
TCATTTCTGG CTAACTTAAA GCTCCATACT AAGGATTCAA GAATGAAAAA AGAATTAAAT 
TTTTCCATGG ATCAAAGCAC TTCTTCAGAA AAGAAAATAC ATTTTAATGA CTTCATAAAA 
AGATTGAAAA CAGGAAAATC TAATGCTTTT CCTACAGGAC CTGATAAAGA AAATTTTGAA 
ATATCTGTTC ATCCCATAAA AACAAAGAAG GCCTCTTCTG TACATCAAAT GCATGTATCT 
CTTAGGGTAG ATGAAAATAG TAGTAATACA ACTAGAATCT TCGGAGAACA AAATGATGGG 
ATGAATTTCA CTCAGTGTCA TACAGCCAGT ATTCAGACTT TGGTTCCCAC ATCCAGTAAG 
GCCAGCATAG GGGAATTTAA AGGTGATGAT ATTACAATTT ATGGCAGTGA TTTTATGGAC 
TTGACAACTA ACCACACTAT ACAGAATTTA CCCTCAGCAG ATAATTCATC TAATAAAGAA 
AATCAAACTC AGAATGTCAT GATGGAGGTT AAAGCTCCAG GAGGGAAGAC AATCTTTAAT 
AAACTGAATA TTCCTTTTCA AACCCCTTTA AACCCTAATG GTGAAATACA TATTACCAAA 
AGTCATATTA CAGGGACAGA AACTCACACA AGCACACAGA CTTCTAACCA AGATGCCAGA 
ACATTGACTA TAATCCCAGA ATCTATATGT TCCAGTCCAG CTACTCAAGA TTATAAGACA 
TTTTTCTATT CTAGCAGTAA TAATGCCATG GAACTGACCA CGTGTCTCTC GGGTATGAGA 
AAAGAGAAGA ATTTGCTGAA GAATGATAAT TATTCTAAAA TGTATCCCAA TCCAGATGCC 
ATCTCTCTTC TTACAGAGAA AACTATTTAT TCAGGAGAGG ATAGCATGGA CATTACGAGG 
AGTCATACAG TTGCAATAGA TAATCAGATT TTTAAACAAG ATCAGACAAA TATACAGAAA 
GCAGACACAT CAATATCTGA AAAAGAAATA ATGCTCCAAA ATCTCATAAC CATGTCAAAG 
GACGGGAAAA TGAATATAAA TTGTAGCTCA GTTCCTCATA TATCTAAGGG AAGATTACAG 
CAGAGCCTGG CAAACTCTTT ATCTATTTCA TTGACTGACA AAAAGACTGA AATCTTCACA 
GGTGAAGATA TGGATTTGAC TAAAAGTCAC ACAACTAACT TAGGAAGTCA AGCTCCACTT 
ACATCTTACA ATGTAACATC TGAGAATACC AGTAAATTTC TCTCTCACAG CAAAAGCCCT 
TCAGGTGAAT GGCAAGAAAT GACCAAAAGT CGTATTGAAC CATCCCAGCA GCCAGACATA 
ATATCTAAAA ACATCCCAGT GGACACCTGG GGCAAAGAAA AAGTTCAAGT TTTAGAGATT 
GCACCTTATG TTGATAAAGA TTCTCCTCAA TCAGCTGATA TTAATCAAGA CATTGCAATA 
AGCCATAGTA TAGTATACTC TGATAGAAAT CTTGAGAAAC AAGTAGCACT AGGAAATAAT 
AGAAATACTG TTCCATGTGA GCAATCTTTG TTTCCTACTT CAGAGTCATT ATTTTCATTA 
GAAGGACAGT CTAACATGAA AAATCATAAT ATTGCTATTA ACAGTTACAC AATGAAATCT 
GTGCCAGGCC AGAATTCTAA ACTGCCTGAG CCACTGAGGA AAGGTGTAGG CAATCCAACA 
CCTGACTATT CTCATGACAA AACAATTATT TGTTCAGAGG AGGAACAAAA TATGGATCTA 
ACCAAGAGTC ACACTGTTGT CATTGGATTT AGTCCTTCTG AAATACAAGA ATTGGGTAAG 
ACTAATCTAG AAAATACTAA CAGTCTGCTA ACAACAGCAA ACAGACAGAC AGCTATAAAA 
GTTGAAAAAT GTTGTAAAAG TCCTATAGAA AAAACAGGAA TATCTGTTTC TAAGGATAAC 
CTAGATGTGA TAGAGGACAA TAGCATACAG AAACTTGGAT TTTTGAATGA AAAGCAAGAT 
GTCAAAATTT GTGGAAGAAA AAGTGTTGGC AGACAAAAAG TTGATAAGAC TATTGTATTT 
TCAGAGGGTG ATGAGAATGA TATGGATATC ACCAAGATTT TTACAGTGGA AATAAATCAT 
AAACCTTTAT TACTTGAACA AGATTCCCAT TTGGCAGGAA CTTCTAAAAC TGTTTTGTAT 
GAATGTGGGC AAGATGATGT GGAGATCACT AAAAGTCACA CAACTGCCTT GGAATGTATA 
ACTGTCTCAC CAGATAAAAT AACCAGTAGG CCTATGGACA AAACTGTAAT GTTTGTAGAT 
AATTACAATG AACTGGAAAT GACAAAGTCT CACACTGTTT TCATTGACTA CCAAGCAAAG 
GAGAGGACTG TGAGTATAGA CAAACTTGAC ACGGAACTAT CCAAAAGAAG AAAAAGCATA 
GAAAAGCCAA AAATGACACC TGCTTCTGCT GAGGAGAGTG TTTCCTTTCT GGAGAATGGT 
GAAAGTGACC ATTCAGCAGC AAACGTCAAT AAGCTAACAC TACTGAGGGA ATGGTCTAAT 
AATGGTCCTA TAGAGAAAGC AGTAGCTGGT GATAACATGG AAGTATCTAA ATTCACCATT 
TGGAAAAATG GCAAAGATGT ACAAAAGCCT GAATTTCTGA ATGACACTAT ATCAGACAAA 
AGTCAGAGAA GGAAAAGCCT TAGACTCAAA AATGACAAGA TTGCTGCATT TTCTAAAAAT 
GATGATGATA TGGATATCAC CCAGAGTTGT ACAGTGAAAA TAAACTATAA AAGTGCCCCA 
GAAGATAGAG AGGATTCCCA TTTGGTGCCT TTGACAGGAG CTTCTAAAAC TGTTTTGTAT 
TCATGTGGGC AGGATGACAT GGAGATGACT AGAAGTCATA CGACTGCGTT AGAATGTAAA 
ACTGTCTCAC CGGATAAAAT CACTACTAGG CCCATAGATA AAACTGTAAT GTTTGTTGAT 
AATCATGGTG ATCTGGAAGT CACCGAGTCC CATACTGTTT TCATTGATTG TCAAAACACA 
GAGAAAATTA ATCAAGAGTG CCCTAAATTT GGAATAGCTA CAGGAAAACC CTTGGGTATT 
TCTTTTCATA ATGATGGTAG CTCTGTTCAA GAAATCACTG AAAAACAAGC ATTGGCAGTA 
GACCAGACCT ATATACCGGA AAAAGCCAGA ATTGGAAGCT GTCAGTTAAA TAGTACAGAT 
AGAAGAAACA TGGACTTTAC TAACAGTCAA GCAACTGCTA CGTGTGGATC CAGTGATAAT 
TATTCCTGTT TACCAAATGC TATTTCCTGT TTTGATAATT TAGAGGGGAA TGTCATGTCC 
TTAAGTAATA AAGATAAGAA AGCCAGTAAC TGCCCAGTGC AAAATGATCT TTCTTATGTA 
AAGGATTTAG CCAATGAATA TTACTTGAAA CCTGAGGGAC TGCCTCCCTC TGCTTGTTCT 
TTGTTAAAGA AAGAAAAAGT GACTCAAACC AATACCAATG GACAGTTAGA CTGTGTTACA 
ACAGTGCTTA AAGATCAAGA TATGATTAAG GAGTCCCAGA ATCTAATAGC TAATCAAACT 
TTACAAGATA GTCAGGAGCT GGCGGAGATG ATTAAACCTA ATTCAAATCG AGTATCCTTT 
AAACCCCCAA AGGATCAAAT GGAGGGCTTT GTTGATACCA CGGAACATAA TTTAAAGAAG 
ACTGTTGTTG ATAATGTTTG TGTTACTTCT CAGCCTCATC TCACAAGCCA GGTACCTCCA 
TTACCTCAAC AAGGACGGAT AATTTTGAAT AAAGATGAAA CAATATCATC TAATTCTGGA 
AATAAGAATT TAAATATTGT TATAGGAAAT TCCTTTGTAC CCACATGTGA AAACGAGGCC 
AAAATGCTCA ACAATGATAA ACAATTTACC ATAGTCTGTA AAAATGAGCT GAGGAAAAAT 
ACTAAATGTA ATACCGATTT CCACAGTAAT TCAGACTTGA CTAAGCAAGT TATTCAAACT 
CATACTAATT CTAGAGAAGC ATCAGATACT GTAATTGCAT CTAATACAAG TTTTAATAGT 
GTCAGATCAA ATCTGAATAA TTTGAATGGA AAAACTGAAG AATTTTTAGA TTTCCAAACT 
CCCCATATTC CATCTTCTCC AGAGCAAATA CTTGCATTAA TAAACAAGGC ACACAATCAT 
ATGAGTATAT TGCAAGCTAC AGAAATACAG AACATTAACC CAGCGCCCAG CAATGTTGAA 
GATGATAAAG ATGAAGAAAG CAAAATTTCT CATAATGGAG CTGAAACCAA CTCTGTACCA 
CTCATGACAG TTGTAAAAGA TAAAGTGAAG AGATGTTCTT TGGGAATATT TTTGCCCAGA 
TTGCCAAACA AGAGAAATTG TAGTGTCACT GGTATTAATG ACCTGAAGCA GATTCAAGGA 
GACACAACTG ATTTAAATCA CTTAGAAATT CAGCCAGTCT TCAGTAAGGA GCCAAGCATT 
GAATTTGTTG CAACTAAACT GACCTTAAGT CCATCTCAGT ATATAAATGA GGAAAACTTT 
CCTATATATC CTGGTGAGAT TAATTCCTCA GATTCTATTA CCATAGAAAC TGAGGAAAAG 
GCTTTGATTG AGACATATCA AAAAGGGATT CCACCATCTG AAAATAAGAT AAGAGAAACC 
TGCAGTAACC AAAAAAGAAC CTGGGTACAA GAAGATGATG ATATTCAGAA TGAGAAAAAA 
ATCAGGAAAA GTGAGATTAG GTGTAGTGAT ACTACACAAG ATCAGGAGAT TTTTGATCAC 
CATGCTGAAG GGGATATAGA TAAAAATGCT AACAGCCTGA GCAGAACCCC ATCTAGTTGC 
AGCAGTTCTC TGGATTCGAT AAAGGCTGAT GGGACCTCTC TGGACTTCAG CACACAGCAC 
AATAGTCAAA TGGAATCACA GTTTCTCAGA GACACTATTT GTGAAGAGAG CTTGAAGGAG 
AAACTCAAAG ATGGGAAAAT TACAATAAAG GAGTTCTTTA TACTTCTTCA GGTCCACATT 
TTGATACAGA AACCCCGACA GAGCAATCTC CCAGCCAAAT TTACTATAAA CACACCACCT 
ACTCTGGAAG ACCTGATGTT AAGTCAGTAT GTTTACCGAC CCAAGATACA GATTTATAGA 
GAAGATTGTG AAGCTCTTTG CCAAAAGATT GAAGAATTAA AGCTTTCTGC ATTGAACCAG 
GATAAATTGT TGACTGACAT AAATAGGAAC CTGTGGGAAA AAATGAGACA CTGCTCTGAT 
GAAGAGCTGA AGGTCTTTGG AATTTACCTG AACAAAATAA AATCACATTT TACCAAGATG 
ACTAAAGTTT TTACTCACCA AGGAAAAGTG GCTCTCTATA CCAAGCTGGT ACAGTCAGCT 
CAGAATGAGA GGAAGAAACT TCAGATAAGG ATAGATGAGA TGGACAACAT ACTTAAGAAG 
ATTGAGAACT GTCTCGCTGA AGTTGAAATA GAAACTAAGA ATTTGGATGA AGAGAAAGAC 
AATCCTATGG AAGAATGGGA TTCTGAAATG AGAGCCGCAG AGAAAGAACT AGAACAGCTG 
AAAACTGAAG AAGATGAGCT CCAAAGAAAT CTCTTAGAAC TGGAAGTACA GAAAGAGCAG 
ACCCTTGCTC AAATAGACTT TGTACAAAAA CAAACAGATA GAACTGAAGA GCTAATGGAT 
CAGTTGAGCT TGTCTGAGTG GGAAGCCATT GAGTGGAGTG ATGATCAAGC TGTATTCACC 
TTTCTTTATG ACACAATAGA ACTTACTATC ACCTTTGGAG AGACAGTAGC TGGAAGTCCC 
AAGATCAGGC TGCCAGCATG GTCAGTTGAT CTCCCTTTCC TGGACAAGGC TTGTAGGAAG 
ATTGTTGACT TGAATTTTCA ATCTCGGTTA GATGAGGATA AAGCTCCTCC TTCCTCCCTT 
TTAGTTCATA AGCTTATTTT CCAGTACATT GAGGAAAAGG AATCCTGGAA GAAAAAATGT 
ACAACACAGC ATCAAATACC CAAGATGCTT CAAGAAATCT CATTGGTAGT GAGCCATTGT 
AGACTCCTTG GAGAGGAAAT TGAGTTTTTA AAGAGATGGG GACCAAATTA TAACCTAATG 
AACATAGATG TGACTAATAC TGAATTGAGG CTTTTATTCT CCAGCTCTGC AGCATTTGCA 
AAATTTGAGA TAACTTTGCC TCTCTCAGTC CATTTTCCAA CTGTTCCATT ACCTTTCTCC 
ATTCAAAAGC ACCTTGGAAA CATTGGCCAA GATGAAATCA CTGCAATTCT ATCTAAAGTG 
CCACTGAAGA ACAACTATCT AAAGAATGTA GTCAAGCAAA TTTACCAAGA TCTGCTTCAG 
GACTGCCATT TCTATCACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013965144.1
CDS	42..6902
Translation	MDGMSSETNEENDNMERPVRRRHSSILKPPRSPLQDLRGGNEAVQESNALRNKKSSRRVSFADTIKVFQTESHMKIVRKSEIAETEAGENVLYIQNKKPEETYCEITGMNTLLSAPIQTQMQQREISITEHNHEMKPANDQTVIFSDENQMDLTASHTVMITKDLLDCTKSEKSIKIDTASFLANLKLHTKDSRMKKELNFSMDQSTSSEKKIHFNDFIKRLKTGKSNAFPTGPDKENFEISVHPIKTKKASSVHQMHVSLRVDENSSNTTRIFGEQNDGMNFTQCHTASIQTLVPTSSKASIGEFKGDDITIYGSDFMDLTTNHTIQNLPSADNSSNKENQTQNVMMEVKAPGGKTIFNKLNIPFQTPLNPNGEIHITKSHITGTETHTSTQTSNQDARTLTIIPESICSSPATQDYKTFFYSSSNNAMELTTCLSGMRKEKNLLKNDNYSKMYPNPDAISLLTEKTIYSGEDSMDITRSHTVAIDNQIFKQDQTNIQKADTSISEKEIMLQNLITMSKDGKMNINCSSVPHISKGRLQQSLANSLSISLTDKKTEIFTGEDMDLTKSHTTNLGSQAPLTSYNVTSENTSKFLSHSKSPSGEWQEMTKSRIEPSQQPDIISKNIPVDTWGKEKVQVLEIAPYVDKDSPQSADINQDIAISHSIVYSDRNLEKQVALGNNRNTVPCEQSLFPTSESLFSLEGQSNMKNHNIAINSYTMKSVPGQNSKLPEPLRKGVGNPTPDYSHDKTIICSEEEQNMDLTKSHTVVIGFSPSEIQELGKTNLENTNSLLTTANRQTAIKVEKCCKSPIEKTGISVSKDNLDVIEDNSIQKLGFLNEKQDVKICGRKSVGRQKVDKTIVFSEGDENDMDITKIFTVEINHKPLLLEQDSHLAGTSKTVLYECGQDDVEITKSHTTALECITVSPDKITSRPMDKTVMFVDNYNELEMTKSHTVFIDYQAKERTVSIDKLDTELSKRRKSIEKPKMTPASAEESVSFLENGESDHSAANVNKLTLLREWSNNGPIEKAVAGDNMEVSKFTIWKNGKDVQKPEFLNDTISDKSQRRKSLRLKNDKIAAFSKNDDDMDITQSCTVKINYKSAPEDREDSHLVPLTGASKTVLYSCGQDDMEMTRSHTTALECKTVSPDKITTRPIDKTVMFVDNHGDLEVTESHTVFIDCQNTEKINQECPKFGIATGKPLGISFHNDGSSVQEITEKQALAVDQTYIPEKARIGSCQLNSTDRRNMDFTNSQATATCGSSDNYSCLPNAISCFDNLEGNVMSLSNKDKKASNCPVQNDLSYVKDLANEYYLKPEGLPPSACSLLKKEKVTQTNTNGQLDCVTTVLKDQDMIKESQNLIANQTLQDSQELAEMIKPNSNRVSFKPPKDQMEGFVDTTEHNLKKTVVDNVCVTSQPHLTSQVPPLPQQGRIILNKDETISSNSGNKNLNIVIGNSFVPTCENEAKMLNNDKQFTIVCKNELRKNTKCNTDFHSNSDLTKQVIQTHTNSREASDTVIASNTSFNSVRSNLNNLNGKTEEFLDFQTPHIPSSPEQILALINKAHNHMSILQATEIQNINPAPSNVEDDKDEESKISHNGAETNSVPLMTVVKDKVKRCSLGIFLPRLPNKRNCSVTGINDLKQIQGDTTDLNHLEIQPVFSKEPSIEFVATKLTLSPSQYINEENFPIYPGEINSSDSITIETEEKALIETYQKGIPPSENKIRETCSNQKRTWVQEDDDIQNEKKIRKSEIRCSDTTQDQEIFDHHAEGDIDKNANSLSRTPSSCSSSLDSIKADGTSLDFSTQHNSQMESQFLRDTICEESLKEKLKDGKITIKEFFILLQVHILIQKPRQSNLPAKFTINTPPTLEDLMLSQYVYRPKIQIYREDCEALCQKIEELKLSALNQDKLLTDINRNLWEKMRHCSDEELKVFGIYLNKIKSHFTKMTKVFTHQGKVALYTKLVQSAQNERKKLQIRIDEMDNILKKIENCLAEVEIETKNLDEEKDNPMEEWDSEMRAAEKELEQLKTEEDELQRNLLELEVQKEQTLAQIDFVQKQTDRTEELMDQLSLSEWEAIEWSDDQAVFTFLYDTIELTITFGETVAGSPKIRLPAWSVDLPFLDKACRKIVDLNFQSRLDEDKAPPSSLLVHKLIFQYIEEKESWKKKCTTQHQIPKMLQEISLVVSHCRLLGEEIEFLKRWGPNYNLMNIDVTNTELRLLFSSSAAFAKFEITLPLSVHFPTVPLPFSIQKHLGNIGQDEITAILSKVPLKNNYLKNVVKQIYQDLLQDCHFYH

Target ORF information:

RefSeq Version	XM_014109669.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris cancer susceptibility candidate 5 (CASC5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014109669.1

ORF Insert Sequence:

ATGGATGGGA TGTCTTCAGA GACTAATGAA GAGAATGACA ATATGGAGAG ACCTGTTAGA 
AGACGACATT CCTCCATTTT GAAACCCCCA AGGAGTCCTC TTCAGGACCT CAGAGGTGGG 
AATGAAGCAG TTCAGGAATC TAATGCTTTG AGAAATAAGA AAAGCTCTCG TCGAGTCAGC 
TTTGCAGATA CCATAAAAGT ATTCCAAACA GAGTCTCATA TGAAAATAGT AAGAAAGTCA 
GAAATTGCAG AAACAGAAGC AGGAGAAAAT GTTCTATATA TACAGAATAA GAAGCCAGAA 
GAAACCTACT GTGAAATTAC TGGGATGAAC ACATTGCTTT CTGCTCCTAT TCAGACTCAG 
ATGCAACAGA GAGAGATTTC AATTACAGAA CACAACCATG AAATGAAACC TGCAAATGAC 
CAAACAGTCA TCTTTTCAGA TGAAAACCAG ATGGACCTAA CAGCAAGTCA CACCGTAATG 
ATTACCAAAG ACCTGTTAGA TTGTACTAAA AGTGAAAAGT CCATTAAGAT AGATACCGCA 
TCATTTCTGG CTAACTTAAA GCTCCATACT AAGGATTCAA GAATGAAAAA AGAATTAAAT 
TTTTCCATGG ATCAAAGCAC TTCTTCAGAA AAGAAAATAC ATTTTAATGA CTTCATAAAA 
AGATTGAAAA CAGGAAAATC TAATGCTTTT CCTACAGGAC CTGATAAAGA AAATTTTGAA 
ATATCTGTTC ATCCCATAAA AACAAAGAAG GCCTCTTCTG TACATCAAAT GCATGTATCT 
CTTAGGGTAG ATGAAAATAG TAGTAATACA ACTAGAATCT TCGGAGAACA AAATGATGGG 
ATGAATTTCA CTCAGTGTCA TACAGCCAGT ATTCAGACTT TGGTTCCCAC ATCCAGTAAG 
GCCAGCATAG GGGAATTTAA AGGTGATGAT ATTACAATTT ATGGCAGTGA TTTTATGGAC 
TTGACAACTA ACCACACTAT ACAGAATTTA CCCTCAGCAG ATAATTCATC TAATAAAGAA 
AATCAAACTC AGAATGTCAT GATGGAGGTT AAAGCTCCAG GAGGGAAGAC AATCTTTAAT 
AAACTGAATA TTCCTTTTCA AACCCCTTTA AACCCTAATG GTGAAATACA TATTACCAAA 
AGTCATATTA CAGGGACAGA AACTCACACA AGCACACAGA CTTCTAACCA AGATGCCAGA 
ACATTGACTA TAATCCCAGA ATCTATATGT TCCAGTCCAG CTACTCAAGA TTATAAGACA 
TTTTTCTATT CTAGCAGTAA TAATGCCATG GAACTGACCA CGTGTCTCTC GGGTATGAGA 
AAAGAGAAGA ATTTGCTGAA GAATGATAAT TATTCTAAAA TGTATCCCAA TCCAGATGCC 
ATCTCTCTTC TTACAGAGAA AACTATTTAT TCAGGAGAGG ATAGCATGGA CATTACGAGG 
AGTCATACAG TTGCAATAGA TAATCAGATT TTTAAACAAG ATCAGACAAA TATACAGAAA 
GCAGACACAT CAATATCTGA AAAAGAAATA ATGCTCCAAA ATCTCATAAC CATGTCAAAG 
GACGGGAAAA TGAATATAAA TTGTAGCTCA GTTCCTCATA TATCTAAGGG AAGATTACAG 
CAGAGCCTGG CAAACTCTTT ATCTATTTCA TTGACTGACA AAAAGACTGA AATCTTCACA 
GGTGAAGATA TGGATTTGAC TAAAAGTCAC ACAACTAACT TAGGAAGTCA AGCTCCACTT 
ACATCTTACA ATGTAACATC TGAGAATACC AGTAAATTTC TCTCTCACAG CAAAAGCCCT 
TCAGGTGAAT GGCAAGAAAT GACCAAAAGT CGTATTGAAC CATCCCAGCA GCCAGACATA 
ATATCTAAAA ACATCCCAGT GGACACCTGG GGCAAAGAAA AAGTTCAAGT TTTAGAGATT 
GCACCTTATG TTGATAAAGA TTCTCCTCAA TCAGCTGATA TTAATCAAGA CATTGCAATA 
AGCCATAGTA TAGTATACTC TGATAGAAAT CTTGAGAAAC AAGTAGCACT AGGAAATAAT 
AGAAATACTG TTCCATGTGA GCAATCTTTG TTTCCTACTT CAGAGTCATT ATTTTCATTA 
GAAGGACAGT CTAACATGAA AAATCATAAT ATTGCTATTA ACAGTTACAC AATGAAATCT 
GTGCCAGGCC AGAATTCTAA ACTGCCTGAG CCACTGAGGA AAGGTGTAGG CAATCCAACA 
CCTGACTATT CTCATGACAA AACAATTATT TGTTCAGAGG AGGAACAAAA TATGGATCTA 
ACCAAGAGTC ACACTGTTGT CATTGGATTT AGTCCTTCTG AAATACAAGA ATTGGGTAAG 
ACTAATCTAG AAAATACTAA CAGTCTGCTA ACAACAGCAA ACAGACAGAC AGCTATAAAA 
GTTGAAAAAT GTTGTAAAAG TCCTATAGAA AAAACAGGAA TATCTGTTTC TAAGGATAAC 
CTAGATGTGA TAGAGGACAA TAGCATACAG AAACTTGGAT TTTTGAATGA AAAGCAAGAT 
GTCAAAATTT GTGGAAGAAA AAGTGTTGGC AGACAAAAAG TTGATAAGAC TATTGTATTT 
TCAGAGGGTG ATGAGAATGA TATGGATATC ACCAAGATTT TTACAGTGGA AATAAATCAT 
AAACCTTTAT TACTTGAACA AGATTCCCAT TTGGCAGGAA CTTCTAAAAC TGTTTTGTAT 
GAATGTGGGC AAGATGATGT GGAGATCACT AAAAGTCACA CAACTGCCTT GGAATGTATA 
ACTGTCTCAC CAGATAAAAT AACCAGTAGG CCTATGGACA AAACTGTAAT GTTTGTAGAT 
AATTACAATG AACTGGAAAT GACAAAGTCT CACACTGTTT TCATTGACTA CCAAGCAAAG 
GAGAGGACTG TGAGTATAGA CAAACTTGAC ACGGAACTAT CCAAAAGAAG AAAAAGCATA 
GAAAAGCCAA AAATGACACC TGCTTCTGCT GAGGAGAGTG TTTCCTTTCT GGAGAATGGT 
GAAAGTGACC ATTCAGCAGC AAACGTCAAT AAGCTAACAC TACTGAGGGA ATGGTCTAAT 
AATGGTCCTA TAGAGAAAGC AGTAGCTGGT GATAACATGG AAGTATCTAA ATTCACCATT 
TGGAAAAATG GCAAAGATGT ACAAAAGCCT GAATTTCTGA ATGACACTAT ATCAGACAAA 
AGTCAGAGAA GGAAAAGCCT TAGACTCAAA AATGACAAGA TTGCTGCATT TTCTAAAAAT 
GATGATGATA TGGATATCAC CCAGAGTTGT ACAGTGAAAA TAAACTATAA AAGTGCCCCA 
GAAGATAGAG AGGATTCCCA TTTGGTGCCT TTGACAGGAG CTTCTAAAAC TGTTTTGTAT 
TCATGTGGGC AGGATGACAT GGAGATGACT AGAAGTCATA CGACTGCGTT AGAATGTAAA 
ACTGTCTCAC CGGATAAAAT CACTACTAGG CCCATAGATA AAACTGTAAT GTTTGTTGAT 
AATCATGGTG ATCTGGAAGT CACCGAGTCC CATACTGTTT TCATTGATTG TCAAAACACA 
GAGAAAATTA ATCAAGAGTG CCCTAAATTT GGAATAGCTA CAGGAAAACC CTTGGGTATT 
TCTTTTCATA ATGATGGTAG CTCTGTTCAA GAAATCACTG AAAAACAAGC ATTGGCAGTA 
GACCAGACCT ATATACCGGA AAAAGCCAGA ATTGGAAGCT GTCAGTTAAA TAGTACAGAT 
AGAAGAAACA TGGACTTTAC TAACAGTCAA GCAACTGCTA CGTGTGGATC CAGTGATAAT 
TATTCCTGTT TACCAAATGC TATTTCCTGT TTTGATAATT TAGAGGGGAA TGTCATGTCC 
TTAAGTAATA AAGATAAGAA AGCCAGTAAC TGCCCAGTGC AAAATGATCT TTCTTATGTA 
AAGGATTTAG CCAATGAATA TTACTTGAAA CCTGAGGGAC TGCCTCCCTC TGCTTGTTCT 
TTGTTAAAGA AAGAAAAAGT GACTCAAACC AATACCAATG GACAGTTAGA CTGTGTTACA 
ACAGTGCTTA AAGATCAAGA TATGATTAAG GAGTCCCAGA ATCTAATAGC TAATCAAACT 
TTACAAGATA GTCAGGAGCT GGCGGAGATG ATTAAACCTA ATTCAAATCG AGTATCCTTT 
AAACCCCCAA AGGATCAAAT GGAGGGCTTT GTTGATACCA CGGAACATAA TTTAAAGAAG 
ACTGTTGTTG ATAATGTTTG TGTTACTTCT CAGCCTCATC TCACAAGCCA GGTACCTCCA 
TTACCTCAAC AAGGACGGAT AATTTTGAAT AAAGATGAAA CAATATCATC TAATTCTGGA 
AATAAGAATT TAAATATTGT TATAGGAAAT TCCTTTGTAC CCACATGTGA AAACGAGGCC 
AAAATGCTCA ACAATGATAA ACAATTTACC ATAGTCTGTA AAAATGAGCT GAGGAAAAAT 
ACTAAATGTA ATACCGATTT CCACAGTAAT TCAGACTTGA CTAAGCAAGT TATTCAAACT 
CATACTAATT CTAGAGAAGC ATCAGATACT GTAATTGCAT CTAATACAAG TTTTAATAGT 
GTCAGATCAA ATCTGAATAA TTTGAATGGA AAAACTGAAG AATTTTTAGA TTTCCAAACT 
CCCCATATTC CATCTTCTCC AGAGCAAATA CTTGCATTAA TAAACAAGGC ACACAATCAT 
ATGAGTATAT TGCAAGCTAC AGAAATACAG AACATTAACC CAGCGCCCAG CAATGTTGAA 
GATGATAAAG ATGAAGAAAG CAAAATTTCT CATAATGGAG CTGAAACCAA CTCTGTACCA 
CTCATGACAG TTGTAAAAGA TAAAGTGAAG AGATGTTCTT TGGGAATATT TTTGCCCAGA 
TTGCCAAACA AGAGAAATTG TAGTGTCACT GGTATTAATG ACCTGAAGCA GATTCAAGGA 
GACACAACTG ATTTAAATCA CTTAGAAATT CAGCCAGTCT TCAGTAAGGA GCCAAGCATT 
GAATTTGTTG CAACTAAACT GACCTTAAGT CCATCTCAGT ATATAAATGA GGAAAACTTT 
CCTATATATC CTGGTGAGAT TAATTCCTCA GATTCTATTA CCATAGAAAC TGAGGAAAAG 
GCTTTGATTG AGACATATCA AAAAGGGATT CCACCATCTG AAAATAAGAT AAGAGAAACC 
TGCAGTAACC AAAAAAGAAC CTGGGTACAA GAAGATGATG ATATTCAGAA TGAGAAAAAA 
ATCAGGAAAA GTGAGATTAG GTGTAGTGAT ACTACACAAG ATCAGGAGAT TTTTGATCAC 
CATGCTGAAG GGGATATAGA TAAAAATGCT AACAGCCTGA GCAGAACCCC ATCTAGTTGC 
AGCAGTTCTC TGGATTCGAT AAAGGCTGAT GGGACCTCTC TGGACTTCAG CACACAGCAC 
AATAGTCAAA TGGAATCACA GTTTCTCAGA GACACTATTT GTGAAGAGAG CTTGAAGGAG 
AAACTCAAAG ATGGGAAAAT TACAATAAAG GAGTTCTTTA TACTTCTTCA GGTCCACATT 
TTGATACAGA AACCCCGACA GAGCAATCTC CCAGCCAAAT TTACTATAAA CACACCACCT 
ACTCTGGAAG ACCTGATGTT AAGTCAGTAT GTTTACCGAC CCAAGATACA GATTTATAGA 
GAAGATTGTG AAGCTCTTTG CCAAAAGATT GAAGAATTAA AGCTTTCTGC ATTGAACCAG 
GATAAATTGT TGACTGACAT AAATAGGAAC CTGTGGGAAA AAATGAGACA CTGCTCTGAT 
GAAGAGCTGA AGGTCTTTGG AATTTACCTG AACAAAATAA AATCACATTT TACCAAGATG 
ACTAAAGTTT TTACTCACCA AGGAAAAGTG GCTCTCTATA CCAAGCTGGT ACAGTCAGCT 
CAGAATGAGA GGAAGAAACT TCAGATAAGG ATAGATGAGA TGGACAACAT ACTTAAGAAG 
ATTGAGAACT GTCTCGCTGA AGTTGAAATA GAAACTAAGA ATTTGGATGA AGAGAAAGAC 
AATCCTATGG AAGAATGGGA TTCTGAAATG AGAGCCGCAG AGAAAGAACT AGAACAGCTG 
AAAACTGAAG AAGATGAGCT CCAAAGAAAT CTCTTAGAAC TGGAAGTACA GAAAGAGCAG 
ACCCTTGCTC AAATAGACTT TGTACAAAAA CAAACAGATA GAACTGAAGA GCTAATGGAT 
CAGTTGAGCT TGTCTGAGTG GGAAGCCATT GAGTGGAGTG ATGATCAAGC TGTATTCACC 
TTTCTTTATG ACACAATAGA ACTTACTATC ACCTTTGGAG AGACAGTAGC TGGAAGTCCC 
AAGATCAGGC TGCCAGCATG GTCAGTTGAT CTCCCTTTCC TGGACAAGGC TTGTAGGAAG 
ATTGTTGACT TGAATTTTCA ATCTCGGTTA GATGAGGATA AAGCTCCTCC TTCCTCCCTT 
TTAGTTCATA AGCTTATTTT CCAGTACATT GAGGAAAAGG AATCCTGGAA GAAAAAATGT 
ACAACACAGC ATCAAATACC CAAGATGCTT CAAGAAATCT CATTGGTAGT GAGCCATTGT 
AGACTCCTTG GAGAGGAAAT TGAGTTTTTA AAGAGATGGG GACCAAATTA TAACCTAATG 
AACATAGATG TGACTAATAC TGAATTGAGG CTTTTATTCT CCAGCTCTGC AGCATTTGCA 
AAATTTGAGA TAACTTTGCC TCTCTCAGTC CATTTTCCAA CTGTTCCATT ACCTTTCTCC 
ATTCAAAAGC ACCTTGGAAA CATTGGCCAA GATGAAATCA CTGCAATTCT ATCTAAAGTG 
CCACTGAAGA ACAACTATCT AAAGAATGTA GTCAAGCAAA TTTACCAAGA TCTGCTTCAG 
GACTGCCATT TCTATCACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KNL1 ( XM_005638255.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005638312.1 Canis lupus familiaris kinetochore scaffold 1 (KNL1), transcript variant X1, mRNA.

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CloneID	OCe28661
Clone ID Related Accession (Same CDS sequence)	XM_014109669.1 , XM_005638255.2 , XM_005638255.1 , XM_014109668.1 , XM_014109668.2
Accession Version	XM_005638255.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6861bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	kinetochore scaffold 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006612.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005638255.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGGGA TGTCTTCAGA GACTAATGAA GAGAATGACA ATATGGAGAG ACCTGTTAGA 
AGACGACATT CCTCCATTTT GAAACCCCCA AGGAGTCCTC TTCAGGACCT CAGAGGTGGG 
AATGAAGCAG TTCAGGAATC TAATGCTTTG AGAAATAAGA AAAGCTCTCG TCGAGTCAGC 
TTTGCAGATA CCATAAAAGT ATTCCAAACA GAGTCTCATA TGAAAATAGT AAGAAAGTCA 
GAAATTGCAG AAACAGAAGC AGGAGAAAAT GTTCTATATA TACAGAATAA GAAGCCAGAA 
GAAACCTACT GTGAAATTAC TGGGATGAAC ACATTGCTTT CTGCTCCTAT TCAGACTCAG 
ATGCAACAGA GAGAGATTTC AATTACAGAA CACAACCATG AAATGAAACC TGCAAATGAC 
CAAACAGTCA TCTTTTCAGA TGAAAACCAG ATGGACCTAA CAGCAAGTCA CACCGTAATG 
ATTACCAAAG ACCTGTTAGA TTGTACTAAA AGTGAAAAGT CCATTAAGAT AGATACCGCA 
TCATTTCTGG CTAACTTAAA GCTCCATACT AAGGATTCAA GAATGAAAAA AGAATTAAAT 
TTTTCCATGG ATCAAAGCAC TTCTTCAGAA AAGAAAATAC ATTTTAATGA CTTCATAAAA 
AGATTGAAAA CAGGAAAATC TAATGCTTTT CCTACAGGAC CTGATAAAGA AAATTTTGAA 
ATATCTGTTC ATCCCATAAA AACAAAGAAG GCCTCTTCTG TACATCAAAT GCATGTATCT 
CTTAGGGTAG ATGAAAATAG TAGTAATACA ACTAGAATCT TCGGAGAACA AAATGATGGG 
ATGAATTTCA CTCAGTGTCA TACAGCCAGT ATTCAGACTT TGGTTCCCAC ATCCAGTAAG 
GCCAGCATAG GGGAATTTAA AGGTGATGAT ATTACAATTT ATGGCAGTGA TTTTATGGAC 
TTGACAACTA ACCACACTAT ACAGAATTTA CCCTCAGCAG ATAATTCATC TAATAAAGAA 
AATCAAACTC AGAATGTCAT GATGGAGGTT AAAGCTCCAG GAGGGAAGAC AATCTTTAAT 
AAACTGAATA TTCCTTTTCA AACCCCTTTA AACCCTAATG GTGAAATACA TATTACCAAA 
AGTCATATTA CAGGGACAGA AACTCACACA AGCACACAGA CTTCTAACCA AGATGCCAGA 
ACATTGACTA TAATCCCAGA ATCTATATGT TCCAGTCCAG CTACTCAAGA TTATAAGACA 
TTTTTCTATT CTAGCAGTAA TAATGCCATG GAACTGACCA CGTGTCTCTC GGGTATGAGA 
AAAGAGAAGA ATTTGCTGAA GAATGATAAT TATTCTAAAA TGTATCCCAA TCCAGATGCC 
ATCTCTCTTC TTACAGAGAA AACTATTTAT TCAGGAGAGG ATAGCATGGA CATTACGAGG 
AGTCATACAG TTGCAATAGA TAATCAGATT TTTAAACAAG ATCAGACAAA TATACAGAAA 
GCAGACACAT CAATATCTGA AAAAGAAATA ATGCTCCAAA ATCTCATAAC CATGTCAAAG 
GACGGGAAAA TGAATATAAA TTGTAGCTCA GTTCCTCATA TATCTAAGGG AAGATTACAG 
CAGAGCCTGG CAAACTCTTT ATCTATTTCA TTGACTGACA AAAAGACTGA AATCTTCACA 
GGTGAAGATA TGGATTTGAC TAAAAGTCAC ACAACTAACT TAGGAAGTCA AGCTCCACTT 
ACATCTTACA ATGTAACATC TGAGAATACC AGTAAATTTC TCTCTCACAG CAAAAGCCCT 
TCAGGTGAAT GGCAAGAAAT GACCAAAAGT CGTATTGAAC CATCCCAGCA GCCAGACATA 
ATATCTAAAA ACATCCCAGT GGACACCTGG GGCAAAGAAA AAGTTCAAGT TTTAGAGATT 
GCACCTTATG TTGATAAAGA TTCTCCTCAA TCAGCTGATA TTAATCAAGA CATTGCAATA 
AGCCATAGTA TAGTATACTC TGATAGAAAT CTTGAGAAAC AAGTAGCACT AGGAAATAAT 
AGAAATACTG TTCCATGTGA GCAATCTTTG TTTCCTACTT CAGAGTCATT ATTTTCATTA 
GAAGGACAGT CTAACATGAA AAATCATAAT ATTGCTATTA ACAGTTACAC AATGAAATCT 
GTGCCAGGCC AGAATTCTAA ACTGCCTGAG CCACTGAGGA AAGGTGTAGG CAATCCAACA 
CCTGACTATT CTCATGACAA AACAATTATT TGTTCAGAGG AGGAACAAAA TATGGATCTA 
ACCAAGAGTC ACACTGTTGT CATTGGATTT AGTCCTTCTG AAATACAAGA ATTGGGTAAG 
ACTAATCTAG AAAATACTAA CAGTCTGCTA ACAACAGCAA ACAGACAGAC AGCTATAAAA 
GTTGAAAAAT GTTGTAAAAG TCCTATAGAA AAAACAGGAA TATCTGTTTC TAAGGATAAC 
CTAGATGTGA TAGAGGACAA TAGCATACAG AAACTTGGAT TTTTGAATGA AAAGCAAGAT 
GTCAAAATTT GTGGAAGAAA AAGTGTTGGC AGACAAAAAG TTGATAAGAC TATTGTATTT 
TCAGAGGGTG ATGAGAATGA TATGGATATC ACCAAGATTT TTACAGTGGA AATAAATCAT 
AAACCTTTAT TACTTGAACA AGATTCCCAT TTGGCAGGAA CTTCTAAAAC TGTTTTGTAT 
GAATGTGGGC AAGATGATGT GGAGATCACT AAAAGTCACA CAACTGCCTT GGAATGTATA 
ACTGTCTCAC CAGATAAAAT AACCAGTAGG CCTATGGACA AAACTGTAAT GTTTGTAGAT 
AATTACAATG AACTGGAAAT GACAAAGTCT CACACTGTTT TCATTGACTA CCAAGCAAAG 
GAGAGGACTG TGAGTATAGA CAAACTTGAC ACGGAACTAT CCAAAAGAAG AAAAAGCATA 
GAAAAGCCAA AAATGACACC TGCTTCTGCT GAGGAGAGTG TTTCCTTTCT GGAGAATGGT 
GAAAGTGACC ATTCAGCAGC AAACGTCAAT AAGCTAACAC TACTGAGGGA ATGGTCTAAT 
AATGGTCCTA TAGAGAAAGC AGTAGCTGGT GATAACATGG AAGTATCTAA ATTCACCATT 
TGGAAAAATG GCAAAGATGT ACAAAAGCCT GAATTTCTGA ATGACACTAT ATCAGACAAA 
AGTCAGAGAA GGAAAAGCCT TAGACTCAAA AATGACAAGA TTGCTGCATT TTCTAAAAAT 
GATGATGATA TGGATATCAC CCAGAGTTGT ACAGTGAAAA TAAACTATAA AAGTGCCCCA 
GAAGATAGAG AGGATTCCCA TTTGGTGCCT TTGACAGGAG CTTCTAAAAC TGTTTTGTAT 
TCATGTGGGC AGGATGACAT GGAGATGACT AGAAGTCATA CGACTGCGTT AGAATGTAAA 
ACTGTCTCAC CGGATAAAAT CACTACTAGG CCCATAGATA AAACTGTAAT GTTTGTTGAT 
AATCATGGTG ATCTGGAAGT CACCGAGTCC CATACTGTTT TCATTGATTG TCAAAACACA 
GAGAAAATTA ATCAAGAGTG CCCTAAATTT GGAATAGCTA CAGGAAAACC CTTGGGTATT 
TCTTTTCATA ATGATGGTAG CTCTGTTCAA GAAATCACTG AAAAACAAGC ATTGGCAGTA 
GACCAGACCT ATATACCGGA AAAAGCCAGA ATTGGAAGCT GTCAGTTAAA TAGTACAGAT 
AGAAGAAACA TGGACTTTAC TAACAGTCAA GCAACTGCTA CGTGTGGATC CAGTGATAAT 
TATTCCTGTT TACCAAATGC TATTTCCTGT TTTGATAATT TAGAGGGGAA TGTCATGTCC 
TTAAGTAATA AAGATAAGAA AGCCAGTAAC TGCCCAGTGC AAAATGATCT TTCTTATGTA 
AAGGATTTAG CCAATGAATA TTACTTGAAA CCTGAGGGAC TGCCTCCCTC TGCTTGTTCT 
TTGTTAAAGA AAGAAAAAGT GACTCAAACC AATACCAATG GACAGTTAGA CTGTGTTACA 
ACAGTGCTTA AAGATCAAGA TATGATTAAG GAGTCCCAGA ATCTAATAGC TAATCAAACT 
TTACAAGATA GTCAGGAGCT GGCGGAGATG ATTAAACCTA ATTCAAATCG AGTATCCTTT 
AAACCCCCAA AGGATCAAAT GGAGGGCTTT GTTGATACCA CGGAACATAA TTTAAAGAAG 
ACTGTTGTTG ATAATGTTTG TGTTACTTCT CAGCCTCATC TCACAAGCCA GGTACCTCCA 
TTACCTCAAC AAGGACGGAT AATTTTGAAT AAAGATGAAA CAATATCATC TAATTCTGGA 
AATAAGAATT TAAATATTGT TATAGGAAAT TCCTTTGTAC CCACATGTGA AAACGAGGCC 
AAAATGCTCA ACAATGATAA ACAATTTACC ATAGTCTGTA AAAATGAGCT GAGGAAAAAT 
ACTAAATGTA ATACCGATTT CCACAGTAAT TCAGACTTGA CTAAGCAAGT TATTCAAACT 
CATACTAATT CTAGAGAAGC ATCAGATACT GTAATTGCAT CTAATACAAG TTTTAATAGT 
GTCAGATCAA ATCTGAATAA TTTGAATGGA AAAACTGAAG AATTTTTAGA TTTCCAAACT 
CCCCATATTC CATCTTCTCC AGAGCAAATA CTTGCATTAA TAAACAAGGC ACACAATCAT 
ATGAGTATAT TGCAAGCTAC AGAAATACAG AACATTAACC CAGCGCCCAG CAATGTTGAA 
GATGATAAAG ATGAAGAAAG CAAAATTTCT CATAATGGAG CTGAAACCAA CTCTGTACCA 
CTCATGACAG TTGTAAAAGA TAAAGTGAAG AGATGTTCTT TGGGAATATT TTTGCCCAGA 
TTGCCAAACA AGAGAAATTG TAGTGTCACT GGTATTAATG ACCTGAAGCA GATTCAAGGA 
GACACAACTG ATTTAAATCA CTTAGAAATT CAGCCAGTCT TCAGTAAGGA GCCAAGCATT 
GAATTTGTTG CAACTAAACT GACCTTAAGT CCATCTCAGT ATATAAATGA GGAAAACTTT 
CCTATATATC CTGGTGAGAT TAATTCCTCA GATTCTATTA CCATAGAAAC TGAGGAAAAG 
GCTTTGATTG AGACATATCA AAAAGGGATT CCACCATCTG AAAATAAGAT AAGAGAAACC 
TGCAGTAACC AAAAAAGAAC CTGGGTACAA GAAGATGATG ATATTCAGAA TGAGAAAAAA 
ATCAGGAAAA GTGAGATTAG GTGTAGTGAT ACTACACAAG ATCAGGAGAT TTTTGATCAC 
CATGCTGAAG GGGATATAGA TAAAAATGCT AACAGCCTGA GCAGAACCCC ATCTAGTTGC 
AGCAGTTCTC TGGATTCGAT AAAGGCTGAT GGGACCTCTC TGGACTTCAG CACACAGCAC 
AATAGTCAAA TGGAATCACA GTTTCTCAGA GACACTATTT GTGAAGAGAG CTTGAAGGAG 
AAACTCAAAG ATGGGAAAAT TACAATAAAG GAGTTCTTTA TACTTCTTCA GGTCCACATT 
TTGATACAGA AACCCCGACA GAGCAATCTC CCAGCCAAAT TTACTATAAA CACACCACCT 
ACTCTGGAAG ACCTGATGTT AAGTCAGTAT GTTTACCGAC CCAAGATACA GATTTATAGA 
GAAGATTGTG AAGCTCTTTG CCAAAAGATT GAAGAATTAA AGCTTTCTGC ATTGAACCAG 
GATAAATTGT TGACTGACAT AAATAGGAAC CTGTGGGAAA AAATGAGACA CTGCTCTGAT 
GAAGAGCTGA AGGTCTTTGG AATTTACCTG AACAAAATAA AATCACATTT TACCAAGATG 
ACTAAAGTTT TTACTCACCA AGGAAAAGTG GCTCTCTATA CCAAGCTGGT ACAGTCAGCT 
CAGAATGAGA GGAAGAAACT TCAGATAAGG ATAGATGAGA TGGACAACAT ACTTAAGAAG 
ATTGAGAACT GTCTCGCTGA AGTTGAAATA GAAACTAAGA ATTTGGATGA AGAGAAAGAC 
AATCCTATGG AAGAATGGGA TTCTGAAATG AGAGCCGCAG AGAAAGAACT AGAACAGCTG 
AAAACTGAAG AAGATGAGCT CCAAAGAAAT CTCTTAGAAC TGGAAGTACA GAAAGAGCAG 
ACCCTTGCTC AAATAGACTT TGTACAAAAA CAAACAGATA GAACTGAAGA GCTAATGGAT 
CAGTTGAGCT TGTCTGAGTG GGAAGCCATT GAGTGGAGTG ATGATCAAGC TGTATTCACC 
TTTCTTTATG ACACAATAGA ACTTACTATC ACCTTTGGAG AGACAGTAGC TGGAAGTCCC 
AAGATCAGGC TGCCAGCATG GTCAGTTGAT CTCCCTTTCC TGGACAAGGC TTGTAGGAAG 
ATTGTTGACT TGAATTTTCA ATCTCGGTTA GATGAGGATA AAGCTCCTCC TTCCTCCCTT 
TTAGTTCATA AGCTTATTTT CCAGTACATT GAGGAAAAGG AATCCTGGAA GAAAAAATGT 
ACAACACAGC ATCAAATACC CAAGATGCTT CAAGAAATCT CATTGGTAGT GAGCCATTGT 
AGACTCCTTG GAGAGGAAAT TGAGTTTTTA AAGAGATGGG GACCAAATTA TAACCTAATG 
AACATAGATG TGACTAATAC TGAATTGAGG CTTTTATTCT CCAGCTCTGC AGCATTTGCA 
AAATTTGAGA TAACTTTGCC TCTCTCAGTC CATTTTCCAA CTGTTCCATT ACCTTTCTCC 
ATTCAAAAGC ACCTTGGAAA CATTGGCCAA GATGAAATCA CTGCAATTCT ATCTAAAGTG 
CCACTGAAGA ACAACTATCT AAAGAATGTA GTCAAGCAAA TTTACCAAGA TCTGCTTCAG 
GACTGCCATT TCTATCACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005638312.1
CDS	339..7199
Translation	MDGMSSETNEENDNMERPVRRRHSSILKPPRSPLQDLRGGNEAVQESNALRNKKSSRRVSFADTIKVFQTESHMKIVRKSEIAETEAGENVLYIQNKKPEETYCEITGMNTLLSAPIQTQMQQREISITEHNHEMKPANDQTVIFSDENQMDLTASHTVMITKDLLDCTKSEKSIKIDTASFLANLKLHTKDSRMKKELNFSMDQSTSSEKKIHFNDFIKRLKTGKSNAFPTGPDKENFEISVHPIKTKKASSVHQMHVSLRVDENSSNTTRIFGEQNDGMNFTQCHTASIQTLVPTSSKASIGEFKGDDITIYGSDFMDLTTNHTIQNLPSADNSSNKENQTQNVMMEVKAPGGKTIFNKLNIPFQTPLNPNGEIHITKSHITGTETHTSTQTSNQDARTLTIIPESICSSPATQDYKTFFYSSSNNAMELTTCLSGMRKEKNLLKNDNYSKMYPNPDAISLLTEKTIYSGEDSMDITRSHTVAIDNQIFKQDQTNIQKADTSISEKEIMLQNLITMSKDGKMNINCSSVPHISKGRLQQSLANSLSISLTDKKTEIFTGEDMDLTKSHTTNLGSQAPLTSYNVTSENTSKFLSHSKSPSGEWQEMTKSRIEPSQQPDIISKNIPVDTWGKEKVQVLEIAPYVDKDSPQSADINQDIAISHSIVYSDRNLEKQVALGNNRNTVPCEQSLFPTSESLFSLEGQSNMKNHNIAINSYTMKSVPGQNSKLPEPLRKGVGNPTPDYSHDKTIICSEEEQNMDLTKSHTVVIGFSPSEIQELGKTNLENTNSLLTTANRQTAIKVEKCCKSPIEKTGISVSKDNLDVIEDNSIQKLGFLNEKQDVKICGRKSVGRQKVDKTIVFSEGDENDMDITKIFTVEINHKPLLLEQDSHLAGTSKTVLYECGQDDVEITKSHTTALECITVSPDKITSRPMDKTVMFVDNYNELEMTKSHTVFIDYQAKERTVSIDKLDTELSKRRKSIEKPKMTPASAEESVSFLENGESDHSAANVNKLTLLREWSNNGPIEKAVAGDNMEVSKFTIWKNGKDVQKPEFLNDTISDKSQRRKSLRLKNDKIAAFSKNDDDMDITQSCTVKINYKSAPEDREDSHLVPLTGASKTVLYSCGQDDMEMTRSHTTALECKTVSPDKITTRPIDKTVMFVDNHGDLEVTESHTVFIDCQNTEKINQECPKFGIATGKPLGISFHNDGSSVQEITEKQALAVDQTYIPEKARIGSCQLNSTDRRNMDFTNSQATATCGSSDNYSCLPNAISCFDNLEGNVMSLSNKDKKASNCPVQNDLSYVKDLANEYYLKPEGLPPSACSLLKKEKVTQTNTNGQLDCVTTVLKDQDMIKESQNLIANQTLQDSQELAEMIKPNSNRVSFKPPKDQMEGFVDTTEHNLKKTVVDNVCVTSQPHLTSQVPPLPQQGRIILNKDETISSNSGNKNLNIVIGNSFVPTCENEAKMLNNDKQFTIVCKNELRKNTKCNTDFHSNSDLTKQVIQTHTNSREASDTVIASNTSFNSVRSNLNNLNGKTEEFLDFQTPHIPSSPEQILALINKAHNHMSILQATEIQNINPAPSNVEDDKDEESKISHNGAETNSVPLMTVVKDKVKRCSLGIFLPRLPNKRNCSVTGINDLKQIQGDTTDLNHLEIQPVFSKEPSIEFVATKLTLSPSQYINEENFPIYPGEINSSDSITIETEEKALIETYQKGIPPSENKIRETCSNQKRTWVQEDDDIQNEKKIRKSEIRCSDTTQDQEIFDHHAEGDIDKNANSLSRTPSSCSSSLDSIKADGTSLDFSTQHNSQMESQFLRDTICEESLKEKLKDGKITIKEFFILLQVHILIQKPRQSNLPAKFTINTPPTLEDLMLSQYVYRPKIQIYREDCEALCQKIEELKLSALNQDKLLTDINRNLWEKMRHCSDEELKVFGIYLNKIKSHFTKMTKVFTHQGKVALYTKLVQSAQNERKKLQIRIDEMDNILKKIENCLAEVEIETKNLDEEKDNPMEEWDSEMRAAEKELEQLKTEEDELQRNLLELEVQKEQTLAQIDFVQKQTDRTEELMDQLSLSEWEAIEWSDDQAVFTFLYDTIELTITFGETVAGSPKIRLPAWSVDLPFLDKACRKIVDLNFQSRLDEDKAPPSSLLVHKLIFQYIEEKESWKKKCTTQHQIPKMLQEISLVVSHCRLLGEEIEFLKRWGPNYNLMNIDVTNTELRLLFSSSAAFAKFEITLPLSVHFPTVPLPFSIQKHLGNIGQDEITAILSKVPLKNNYLKNVVKQIYQDLLQDCHFYH

Target ORF information:

RefSeq Version	XM_005638255.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris kinetochore scaffold 1 (KNL1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005638255.2

ORF Insert Sequence:

ATGGATGGGA TGTCTTCAGA GACTAATGAA GAGAATGACA ATATGGAGAG ACCTGTTAGA 
AGACGACATT CCTCCATTTT GAAACCCCCA AGGAGTCCTC TTCAGGACCT CAGAGGTGGG 
AATGAAGCAG TTCAGGAATC TAATGCTTTG AGAAATAAGA AAAGCTCTCG TCGAGTCAGC 
TTTGCAGATA CCATAAAAGT ATTCCAAACA GAGTCTCATA TGAAAATAGT AAGAAAGTCA 
GAAATTGCAG AAACAGAAGC AGGAGAAAAT GTTCTATATA TACAGAATAA GAAGCCAGAA 
GAAACCTACT GTGAAATTAC TGGGATGAAC ACATTGCTTT CTGCTCCTAT TCAGACTCAG 
ATGCAACAGA GAGAGATTTC AATTACAGAA CACAACCATG AAATGAAACC TGCAAATGAC 
CAAACAGTCA TCTTTTCAGA TGAAAACCAG ATGGACCTAA CAGCAAGTCA CACCGTAATG 
ATTACCAAAG ACCTGTTAGA TTGTACTAAA AGTGAAAAGT CCATTAAGAT AGATACCGCA 
TCATTTCTGG CTAACTTAAA GCTCCATACT AAGGATTCAA GAATGAAAAA AGAATTAAAT 
TTTTCCATGG ATCAAAGCAC TTCTTCAGAA AAGAAAATAC ATTTTAATGA CTTCATAAAA 
AGATTGAAAA CAGGAAAATC TAATGCTTTT CCTACAGGAC CTGATAAAGA AAATTTTGAA 
ATATCTGTTC ATCCCATAAA AACAAAGAAG GCCTCTTCTG TACATCAAAT GCATGTATCT 
CTTAGGGTAG ATGAAAATAG TAGTAATACA ACTAGAATCT TCGGAGAACA AAATGATGGG 
ATGAATTTCA CTCAGTGTCA TACAGCCAGT ATTCAGACTT TGGTTCCCAC ATCCAGTAAG 
GCCAGCATAG GGGAATTTAA AGGTGATGAT ATTACAATTT ATGGCAGTGA TTTTATGGAC 
TTGACAACTA ACCACACTAT ACAGAATTTA CCCTCAGCAG ATAATTCATC TAATAAAGAA 
AATCAAACTC AGAATGTCAT GATGGAGGTT AAAGCTCCAG GAGGGAAGAC AATCTTTAAT 
AAACTGAATA TTCCTTTTCA AACCCCTTTA AACCCTAATG GTGAAATACA TATTACCAAA 
AGTCATATTA CAGGGACAGA AACTCACACA AGCACACAGA CTTCTAACCA AGATGCCAGA 
ACATTGACTA TAATCCCAGA ATCTATATGT TCCAGTCCAG CTACTCAAGA TTATAAGACA 
TTTTTCTATT CTAGCAGTAA TAATGCCATG GAACTGACCA CGTGTCTCTC GGGTATGAGA 
AAAGAGAAGA ATTTGCTGAA GAATGATAAT TATTCTAAAA TGTATCCCAA TCCAGATGCC 
ATCTCTCTTC TTACAGAGAA AACTATTTAT TCAGGAGAGG ATAGCATGGA CATTACGAGG 
AGTCATACAG TTGCAATAGA TAATCAGATT TTTAAACAAG ATCAGACAAA TATACAGAAA 
GCAGACACAT CAATATCTGA AAAAGAAATA ATGCTCCAAA ATCTCATAAC CATGTCAAAG 
GACGGGAAAA TGAATATAAA TTGTAGCTCA GTTCCTCATA TATCTAAGGG AAGATTACAG 
CAGAGCCTGG CAAACTCTTT ATCTATTTCA TTGACTGACA AAAAGACTGA AATCTTCACA 
GGTGAAGATA TGGATTTGAC TAAAAGTCAC ACAACTAACT TAGGAAGTCA AGCTCCACTT 
ACATCTTACA ATGTAACATC TGAGAATACC AGTAAATTTC TCTCTCACAG CAAAAGCCCT 
TCAGGTGAAT GGCAAGAAAT GACCAAAAGT CGTATTGAAC CATCCCAGCA GCCAGACATA 
ATATCTAAAA ACATCCCAGT GGACACCTGG GGCAAAGAAA AAGTTCAAGT TTTAGAGATT 
GCACCTTATG TTGATAAAGA TTCTCCTCAA TCAGCTGATA TTAATCAAGA CATTGCAATA 
AGCCATAGTA TAGTATACTC TGATAGAAAT CTTGAGAAAC AAGTAGCACT AGGAAATAAT 
AGAAATACTG TTCCATGTGA GCAATCTTTG TTTCCTACTT CAGAGTCATT ATTTTCATTA 
GAAGGACAGT CTAACATGAA AAATCATAAT ATTGCTATTA ACAGTTACAC AATGAAATCT 
GTGCCAGGCC AGAATTCTAA ACTGCCTGAG CCACTGAGGA AAGGTGTAGG CAATCCAACA 
CCTGACTATT CTCATGACAA AACAATTATT TGTTCAGAGG AGGAACAAAA TATGGATCTA 
ACCAAGAGTC ACACTGTTGT CATTGGATTT AGTCCTTCTG AAATACAAGA ATTGGGTAAG 
ACTAATCTAG AAAATACTAA CAGTCTGCTA ACAACAGCAA ACAGACAGAC AGCTATAAAA 
GTTGAAAAAT GTTGTAAAAG TCCTATAGAA AAAACAGGAA TATCTGTTTC TAAGGATAAC 
CTAGATGTGA TAGAGGACAA TAGCATACAG AAACTTGGAT TTTTGAATGA AAAGCAAGAT 
GTCAAAATTT GTGGAAGAAA AAGTGTTGGC AGACAAAAAG TTGATAAGAC TATTGTATTT 
TCAGAGGGTG ATGAGAATGA TATGGATATC ACCAAGATTT TTACAGTGGA AATAAATCAT 
AAACCTTTAT TACTTGAACA AGATTCCCAT TTGGCAGGAA CTTCTAAAAC TGTTTTGTAT 
GAATGTGGGC AAGATGATGT GGAGATCACT AAAAGTCACA CAACTGCCTT GGAATGTATA 
ACTGTCTCAC CAGATAAAAT AACCAGTAGG CCTATGGACA AAACTGTAAT GTTTGTAGAT 
AATTACAATG AACTGGAAAT GACAAAGTCT CACACTGTTT TCATTGACTA CCAAGCAAAG 
GAGAGGACTG TGAGTATAGA CAAACTTGAC ACGGAACTAT CCAAAAGAAG AAAAAGCATA 
GAAAAGCCAA AAATGACACC TGCTTCTGCT GAGGAGAGTG TTTCCTTTCT GGAGAATGGT 
GAAAGTGACC ATTCAGCAGC AAACGTCAAT AAGCTAACAC TACTGAGGGA ATGGTCTAAT 
AATGGTCCTA TAGAGAAAGC AGTAGCTGGT GATAACATGG AAGTATCTAA ATTCACCATT 
TGGAAAAATG GCAAAGATGT ACAAAAGCCT GAATTTCTGA ATGACACTAT ATCAGACAAA 
AGTCAGAGAA GGAAAAGCCT TAGACTCAAA AATGACAAGA TTGCTGCATT TTCTAAAAAT 
GATGATGATA TGGATATCAC CCAGAGTTGT ACAGTGAAAA TAAACTATAA AAGTGCCCCA 
GAAGATAGAG AGGATTCCCA TTTGGTGCCT TTGACAGGAG CTTCTAAAAC TGTTTTGTAT 
TCATGTGGGC AGGATGACAT GGAGATGACT AGAAGTCATA CGACTGCGTT AGAATGTAAA 
ACTGTCTCAC CGGATAAAAT CACTACTAGG CCCATAGATA AAACTGTAAT GTTTGTTGAT 
AATCATGGTG ATCTGGAAGT CACCGAGTCC CATACTGTTT TCATTGATTG TCAAAACACA 
GAGAAAATTA ATCAAGAGTG CCCTAAATTT GGAATAGCTA CAGGAAAACC CTTGGGTATT 
TCTTTTCATA ATGATGGTAG CTCTGTTCAA GAAATCACTG AAAAACAAGC ATTGGCAGTA 
GACCAGACCT ATATACCGGA AAAAGCCAGA ATTGGAAGCT GTCAGTTAAA TAGTACAGAT 
AGAAGAAACA TGGACTTTAC TAACAGTCAA GCAACTGCTA CGTGTGGATC CAGTGATAAT 
TATTCCTGTT TACCAAATGC TATTTCCTGT TTTGATAATT TAGAGGGGAA TGTCATGTCC 
TTAAGTAATA AAGATAAGAA AGCCAGTAAC TGCCCAGTGC AAAATGATCT TTCTTATGTA 
AAGGATTTAG CCAATGAATA TTACTTGAAA CCTGAGGGAC TGCCTCCCTC TGCTTGTTCT 
TTGTTAAAGA AAGAAAAAGT GACTCAAACC AATACCAATG GACAGTTAGA CTGTGTTACA 
ACAGTGCTTA AAGATCAAGA TATGATTAAG GAGTCCCAGA ATCTAATAGC TAATCAAACT 
TTACAAGATA GTCAGGAGCT GGCGGAGATG ATTAAACCTA ATTCAAATCG AGTATCCTTT 
AAACCCCCAA AGGATCAAAT GGAGGGCTTT GTTGATACCA CGGAACATAA TTTAAAGAAG 
ACTGTTGTTG ATAATGTTTG TGTTACTTCT CAGCCTCATC TCACAAGCCA GGTACCTCCA 
TTACCTCAAC AAGGACGGAT AATTTTGAAT AAAGATGAAA CAATATCATC TAATTCTGGA 
AATAAGAATT TAAATATTGT TATAGGAAAT TCCTTTGTAC CCACATGTGA AAACGAGGCC 
AAAATGCTCA ACAATGATAA ACAATTTACC ATAGTCTGTA AAAATGAGCT GAGGAAAAAT 
ACTAAATGTA ATACCGATTT CCACAGTAAT TCAGACTTGA CTAAGCAAGT TATTCAAACT 
CATACTAATT CTAGAGAAGC ATCAGATACT GTAATTGCAT CTAATACAAG TTTTAATAGT 
GTCAGATCAA ATCTGAATAA TTTGAATGGA AAAACTGAAG AATTTTTAGA TTTCCAAACT 
CCCCATATTC CATCTTCTCC AGAGCAAATA CTTGCATTAA TAAACAAGGC ACACAATCAT 
ATGAGTATAT TGCAAGCTAC AGAAATACAG AACATTAACC CAGCGCCCAG CAATGTTGAA 
GATGATAAAG ATGAAGAAAG CAAAATTTCT CATAATGGAG CTGAAACCAA CTCTGTACCA 
CTCATGACAG TTGTAAAAGA TAAAGTGAAG AGATGTTCTT TGGGAATATT TTTGCCCAGA 
TTGCCAAACA AGAGAAATTG TAGTGTCACT GGTATTAATG ACCTGAAGCA GATTCAAGGA 
GACACAACTG ATTTAAATCA CTTAGAAATT CAGCCAGTCT TCAGTAAGGA GCCAAGCATT 
GAATTTGTTG CAACTAAACT GACCTTAAGT CCATCTCAGT ATATAAATGA GGAAAACTTT 
CCTATATATC CTGGTGAGAT TAATTCCTCA GATTCTATTA CCATAGAAAC TGAGGAAAAG 
GCTTTGATTG AGACATATCA AAAAGGGATT CCACCATCTG AAAATAAGAT AAGAGAAACC 
TGCAGTAACC AAAAAAGAAC CTGGGTACAA GAAGATGATG ATATTCAGAA TGAGAAAAAA 
ATCAGGAAAA GTGAGATTAG GTGTAGTGAT ACTACACAAG ATCAGGAGAT TTTTGATCAC 
CATGCTGAAG GGGATATAGA TAAAAATGCT AACAGCCTGA GCAGAACCCC ATCTAGTTGC 
AGCAGTTCTC TGGATTCGAT AAAGGCTGAT GGGACCTCTC TGGACTTCAG CACACAGCAC 
AATAGTCAAA TGGAATCACA GTTTCTCAGA GACACTATTT GTGAAGAGAG CTTGAAGGAG 
AAACTCAAAG ATGGGAAAAT TACAATAAAG GAGTTCTTTA TACTTCTTCA GGTCCACATT 
TTGATACAGA AACCCCGACA GAGCAATCTC CCAGCCAAAT TTACTATAAA CACACCACCT 
ACTCTGGAAG ACCTGATGTT AAGTCAGTAT GTTTACCGAC CCAAGATACA GATTTATAGA 
GAAGATTGTG AAGCTCTTTG CCAAAAGATT GAAGAATTAA AGCTTTCTGC ATTGAACCAG 
GATAAATTGT TGACTGACAT AAATAGGAAC CTGTGGGAAA AAATGAGACA CTGCTCTGAT 
GAAGAGCTGA AGGTCTTTGG AATTTACCTG AACAAAATAA AATCACATTT TACCAAGATG 
ACTAAAGTTT TTACTCACCA AGGAAAAGTG GCTCTCTATA CCAAGCTGGT ACAGTCAGCT 
CAGAATGAGA GGAAGAAACT TCAGATAAGG ATAGATGAGA TGGACAACAT ACTTAAGAAG 
ATTGAGAACT GTCTCGCTGA AGTTGAAATA GAAACTAAGA ATTTGGATGA AGAGAAAGAC 
AATCCTATGG AAGAATGGGA TTCTGAAATG AGAGCCGCAG AGAAAGAACT AGAACAGCTG 
AAAACTGAAG AAGATGAGCT CCAAAGAAAT CTCTTAGAAC TGGAAGTACA GAAAGAGCAG 
ACCCTTGCTC AAATAGACTT TGTACAAAAA CAAACAGATA GAACTGAAGA GCTAATGGAT 
CAGTTGAGCT TGTCTGAGTG GGAAGCCATT GAGTGGAGTG ATGATCAAGC TGTATTCACC 
TTTCTTTATG ACACAATAGA ACTTACTATC ACCTTTGGAG AGACAGTAGC TGGAAGTCCC 
AAGATCAGGC TGCCAGCATG GTCAGTTGAT CTCCCTTTCC TGGACAAGGC TTGTAGGAAG 
ATTGTTGACT TGAATTTTCA ATCTCGGTTA GATGAGGATA AAGCTCCTCC TTCCTCCCTT 
TTAGTTCATA AGCTTATTTT CCAGTACATT GAGGAAAAGG AATCCTGGAA GAAAAAATGT 
ACAACACAGC ATCAAATACC CAAGATGCTT CAAGAAATCT CATTGGTAGT GAGCCATTGT 
AGACTCCTTG GAGAGGAAAT TGAGTTTTTA AAGAGATGGG GACCAAATTA TAACCTAATG 
AACATAGATG TGACTAATAC TGAATTGAGG CTTTTATTCT CCAGCTCTGC AGCATTTGCA 
AAATTTGAGA TAACTTTGCC TCTCTCAGTC CATTTTCCAA CTGTTCCATT ACCTTTCTCC 
ATTCAAAAGC ACCTTGGAAA CATTGGCCAA GATGAAATCA CTGCAATTCT ATCTAAAGTG 
CCACTGAAGA ACAACTATCT AAAGAATGTA GTCAAGCAAA TTTACCAAGA TCTGCTTCAG 
GACTGCCATT TCTATCACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KNL1 ( XM_005638255.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005638312.1 Canis lupus familiaris cancer susceptibility candidate 5 (CASC5), transcript variant X1, mRNA.

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CloneID	OCe28661
Clone ID Related Accession (Same CDS sequence)	XM_014109669.1 , XM_005638255.2 , XM_005638255.1 , XM_014109668.1 , XM_014109668.2
Accession Version	XM_005638255.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6861bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	protein CASC5 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726113.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGGGA TGTCTTCAGA GACTAATGAA GAGAATGACA ATATGGAGAG ACCTGTTAGA 
AGACGACATT CCTCCATTTT GAAACCCCCA AGGAGTCCTC TTCAGGACCT CAGAGGTGGG 
AATGAAGCAG TTCAGGAATC TAATGCTTTG AGAAATAAGA AAAGCTCTCG TCGAGTCAGC 
TTTGCAGATA CCATAAAAGT ATTCCAAACA GAGTCTCATA TGAAAATAGT AAGAAAGTCA 
GAAATTGCAG AAACAGAAGC AGGAGAAAAT GTTCTATATA TACAGAATAA GAAGCCAGAA 
GAAACCTACT GTGAAATTAC TGGGATGAAC ACATTGCTTT CTGCTCCTAT TCAGACTCAG 
ATGCAACAGA GAGAGATTTC AATTACAGAA CACAACCATG AAATGAAACC TGCAAATGAC 
CAAACAGTCA TCTTTTCAGA TGAAAACCAG ATGGACCTAA CAGCAAGTCA CACCGTAATG 
ATTACCAAAG ACCTGTTAGA TTGTACTAAA AGTGAAAAGT CCATTAAGAT AGATACCGCA 
TCATTTCTGG CTAACTTAAA GCTCCATACT AAGGATTCAA GAATGAAAAA AGAATTAAAT 
TTTTCCATGG ATCAAAGCAC TTCTTCAGAA AAGAAAATAC ATTTTAATGA CTTCATAAAA 
AGATTGAAAA CAGGAAAATC TAATGCTTTT CCTACAGGAC CTGATAAAGA AAATTTTGAA 
ATATCTGTTC ATCCCATAAA AACAAAGAAG GCCTCTTCTG TACATCAAAT GCATGTATCT 
CTTAGGGTAG ATGAAAATAG TAGTAATACA ACTAGAATCT TCGGAGAACA AAATGATGGG 
ATGAATTTCA CTCAGTGTCA TACAGCCAGT ATTCAGACTT TGGTTCCCAC ATCCAGTAAG 
GCCAGCATAG GGGAATTTAA AGGTGATGAT ATTACAATTT ATGGCAGTGA TTTTATGGAC 
TTGACAACTA ACCACACTAT ACAGAATTTA CCCTCAGCAG ATAATTCATC TAATAAAGAA 
AATCAAACTC AGAATGTCAT GATGGAGGTT AAAGCTCCAG GAGGGAAGAC AATCTTTAAT 
AAACTGAATA TTCCTTTTCA AACCCCTTTA AACCCTAATG GTGAAATACA TATTACCAAA 
AGTCATATTA CAGGGACAGA AACTCACACA AGCACACAGA CTTCTAACCA AGATGCCAGA 
ACATTGACTA TAATCCCAGA ATCTATATGT TCCAGTCCAG CTACTCAAGA TTATAAGACA 
TTTTTCTATT CTAGCAGTAA TAATGCCATG GAACTGACCA CGTGTCTCTC GGGTATGAGA 
AAAGAGAAGA ATTTGCTGAA GAATGATAAT TATTCTAAAA TGTATCCCAA TCCAGATGCC 
ATCTCTCTTC TTACAGAGAA AACTATTTAT TCAGGAGAGG ATAGCATGGA CATTACGAGG 
AGTCATACAG TTGCAATAGA TAATCAGATT TTTAAACAAG ATCAGACAAA TATACAGAAA 
GCAGACACAT CAATATCTGA AAAAGAAATA ATGCTCCAAA ATCTCATAAC CATGTCAAAG 
GACGGGAAAA TGAATATAAA TTGTAGCTCA GTTCCTCATA TATCTAAGGG AAGATTACAG 
CAGAGCCTGG CAAACTCTTT ATCTATTTCA TTGACTGACA AAAAGACTGA AATCTTCACA 
GGTGAAGATA TGGATTTGAC TAAAAGTCAC ACAACTAACT TAGGAAGTCA AGCTCCACTT 
ACATCTTACA ATGTAACATC TGAGAATACC AGTAAATTTC TCTCTCACAG CAAAAGCCCT 
TCAGGTGAAT GGCAAGAAAT GACCAAAAGT CGTATTGAAC CATCCCAGCA GCCAGACATA 
ATATCTAAAA ACATCCCAGT GGACACCTGG GGCAAAGAAA AAGTTCAAGT TTTAGAGATT 
GCACCTTATG TTGATAAAGA TTCTCCTCAA TCAGCTGATA TTAATCAAGA CATTGCAATA 
AGCCATAGTA TAGTATACTC TGATAGAAAT CTTGAGAAAC AAGTAGCACT AGGAAATAAT 
AGAAATACTG TTCCATGTGA GCAATCTTTG TTTCCTACTT CAGAGTCATT ATTTTCATTA 
GAAGGACAGT CTAACATGAA AAATCATAAT ATTGCTATTA ACAGTTACAC AATGAAATCT 
GTGCCAGGCC AGAATTCTAA ACTGCCTGAG CCACTGAGGA AAGGTGTAGG CAATCCAACA 
CCTGACTATT CTCATGACAA AACAATTATT TGTTCAGAGG AGGAACAAAA TATGGATCTA 
ACCAAGAGTC ACACTGTTGT CATTGGATTT AGTCCTTCTG AAATACAAGA ATTGGGTAAG 
ACTAATCTAG AAAATACTAA CAGTCTGCTA ACAACAGCAA ACAGACAGAC AGCTATAAAA 
GTTGAAAAAT GTTGTAAAAG TCCTATAGAA AAAACAGGAA TATCTGTTTC TAAGGATAAC 
CTAGATGTGA TAGAGGACAA TAGCATACAG AAACTTGGAT TTTTGAATGA AAAGCAAGAT 
GTCAAAATTT GTGGAAGAAA AAGTGTTGGC AGACAAAAAG TTGATAAGAC TATTGTATTT 
TCAGAGGGTG ATGAGAATGA TATGGATATC ACCAAGATTT TTACAGTGGA AATAAATCAT 
AAACCTTTAT TACTTGAACA AGATTCCCAT TTGGCAGGAA CTTCTAAAAC TGTTTTGTAT 
GAATGTGGGC AAGATGATGT GGAGATCACT AAAAGTCACA CAACTGCCTT GGAATGTATA 
ACTGTCTCAC CAGATAAAAT AACCAGTAGG CCTATGGACA AAACTGTAAT GTTTGTAGAT 
AATTACAATG AACTGGAAAT GACAAAGTCT CACACTGTTT TCATTGACTA CCAAGCAAAG 
GAGAGGACTG TGAGTATAGA CAAACTTGAC ACGGAACTAT CCAAAAGAAG AAAAAGCATA 
GAAAAGCCAA AAATGACACC TGCTTCTGCT GAGGAGAGTG TTTCCTTTCT GGAGAATGGT 
GAAAGTGACC ATTCAGCAGC AAACGTCAAT AAGCTAACAC TACTGAGGGA ATGGTCTAAT 
AATGGTCCTA TAGAGAAAGC AGTAGCTGGT GATAACATGG AAGTATCTAA ATTCACCATT 
TGGAAAAATG GCAAAGATGT ACAAAAGCCT GAATTTCTGA ATGACACTAT ATCAGACAAA 
AGTCAGAGAA GGAAAAGCCT TAGACTCAAA AATGACAAGA TTGCTGCATT TTCTAAAAAT 
GATGATGATA TGGATATCAC CCAGAGTTGT ACAGTGAAAA TAAACTATAA AAGTGCCCCA 
GAAGATAGAG AGGATTCCCA TTTGGTGCCT TTGACAGGAG CTTCTAAAAC TGTTTTGTAT 
TCATGTGGGC AGGATGACAT GGAGATGACT AGAAGTCATA CGACTGCGTT AGAATGTAAA 
ACTGTCTCAC CGGATAAAAT CACTACTAGG CCCATAGATA AAACTGTAAT GTTTGTTGAT 
AATCATGGTG ATCTGGAAGT CACCGAGTCC CATACTGTTT TCATTGATTG TCAAAACACA 
GAGAAAATTA ATCAAGAGTG CCCTAAATTT GGAATAGCTA CAGGAAAACC CTTGGGTATT 
TCTTTTCATA ATGATGGTAG CTCTGTTCAA GAAATCACTG AAAAACAAGC ATTGGCAGTA 
GACCAGACCT ATATACCGGA AAAAGCCAGA ATTGGAAGCT GTCAGTTAAA TAGTACAGAT 
AGAAGAAACA TGGACTTTAC TAACAGTCAA GCAACTGCTA CGTGTGGATC CAGTGATAAT 
TATTCCTGTT TACCAAATGC TATTTCCTGT TTTGATAATT TAGAGGGGAA TGTCATGTCC 
TTAAGTAATA AAGATAAGAA AGCCAGTAAC TGCCCAGTGC AAAATGATCT TTCTTATGTA 
AAGGATTTAG CCAATGAATA TTACTTGAAA CCTGAGGGAC TGCCTCCCTC TGCTTGTTCT 
TTGTTAAAGA AAGAAAAAGT GACTCAAACC AATACCAATG GACAGTTAGA CTGTGTTACA 
ACAGTGCTTA AAGATCAAGA TATGATTAAG GAGTCCCAGA ATCTAATAGC TAATCAAACT 
TTACAAGATA GTCAGGAGCT GGCGGAGATG ATTAAACCTA ATTCAAATCG AGTATCCTTT 
AAACCCCCAA AGGATCAAAT GGAGGGCTTT GTTGATACCA CGGAACATAA TTTAAAGAAG 
ACTGTTGTTG ATAATGTTTG TGTTACTTCT CAGCCTCATC TCACAAGCCA GGTACCTCCA 
TTACCTCAAC AAGGACGGAT AATTTTGAAT AAAGATGAAA CAATATCATC TAATTCTGGA 
AATAAGAATT TAAATATTGT TATAGGAAAT TCCTTTGTAC CCACATGTGA AAACGAGGCC 
AAAATGCTCA ACAATGATAA ACAATTTACC ATAGTCTGTA AAAATGAGCT GAGGAAAAAT 
ACTAAATGTA ATACCGATTT CCACAGTAAT TCAGACTTGA CTAAGCAAGT TATTCAAACT 
CATACTAATT CTAGAGAAGC ATCAGATACT GTAATTGCAT CTAATACAAG TTTTAATAGT 
GTCAGATCAA ATCTGAATAA TTTGAATGGA AAAACTGAAG AATTTTTAGA TTTCCAAACT 
CCCCATATTC CATCTTCTCC AGAGCAAATA CTTGCATTAA TAAACAAGGC ACACAATCAT 
ATGAGTATAT TGCAAGCTAC AGAAATACAG AACATTAACC CAGCGCCCAG CAATGTTGAA 
GATGATAAAG ATGAAGAAAG CAAAATTTCT CATAATGGAG CTGAAACCAA CTCTGTACCA 
CTCATGACAG TTGTAAAAGA TAAAGTGAAG AGATGTTCTT TGGGAATATT TTTGCCCAGA 
TTGCCAAACA AGAGAAATTG TAGTGTCACT GGTATTAATG ACCTGAAGCA GATTCAAGGA 
GACACAACTG ATTTAAATCA CTTAGAAATT CAGCCAGTCT TCAGTAAGGA GCCAAGCATT 
GAATTTGTTG CAACTAAACT GACCTTAAGT CCATCTCAGT ATATAAATGA GGAAAACTTT 
CCTATATATC CTGGTGAGAT TAATTCCTCA GATTCTATTA CCATAGAAAC TGAGGAAAAG 
GCTTTGATTG AGACATATCA AAAAGGGATT CCACCATCTG AAAATAAGAT AAGAGAAACC 
TGCAGTAACC AAAAAAGAAC CTGGGTACAA GAAGATGATG ATATTCAGAA TGAGAAAAAA 
ATCAGGAAAA GTGAGATTAG GTGTAGTGAT ACTACACAAG ATCAGGAGAT TTTTGATCAC 
CATGCTGAAG GGGATATAGA TAAAAATGCT AACAGCCTGA GCAGAACCCC ATCTAGTTGC 
AGCAGTTCTC TGGATTCGAT AAAGGCTGAT GGGACCTCTC TGGACTTCAG CACACAGCAC 
AATAGTCAAA TGGAATCACA GTTTCTCAGA GACACTATTT GTGAAGAGAG CTTGAAGGAG 
AAACTCAAAG ATGGGAAAAT TACAATAAAG GAGTTCTTTA TACTTCTTCA GGTCCACATT 
TTGATACAGA AACCCCGACA GAGCAATCTC CCAGCCAAAT TTACTATAAA CACACCACCT 
ACTCTGGAAG ACCTGATGTT AAGTCAGTAT GTTTACCGAC CCAAGATACA GATTTATAGA 
GAAGATTGTG AAGCTCTTTG CCAAAAGATT GAAGAATTAA AGCTTTCTGC ATTGAACCAG 
GATAAATTGT TGACTGACAT AAATAGGAAC CTGTGGGAAA AAATGAGACA CTGCTCTGAT 
GAAGAGCTGA AGGTCTTTGG AATTTACCTG AACAAAATAA AATCACATTT TACCAAGATG 
ACTAAAGTTT TTACTCACCA AGGAAAAGTG GCTCTCTATA CCAAGCTGGT ACAGTCAGCT 
CAGAATGAGA GGAAGAAACT TCAGATAAGG ATAGATGAGA TGGACAACAT ACTTAAGAAG 
ATTGAGAACT GTCTCGCTGA AGTTGAAATA GAAACTAAGA ATTTGGATGA AGAGAAAGAC 
AATCCTATGG AAGAATGGGA TTCTGAAATG AGAGCCGCAG AGAAAGAACT AGAACAGCTG 
AAAACTGAAG AAGATGAGCT CCAAAGAAAT CTCTTAGAAC TGGAAGTACA GAAAGAGCAG 
ACCCTTGCTC AAATAGACTT TGTACAAAAA CAAACAGATA GAACTGAAGA GCTAATGGAT 
CAGTTGAGCT TGTCTGAGTG GGAAGCCATT GAGTGGAGTG ATGATCAAGC TGTATTCACC 
TTTCTTTATG ACACAATAGA ACTTACTATC ACCTTTGGAG AGACAGTAGC TGGAAGTCCC 
AAGATCAGGC TGCCAGCATG GTCAGTTGAT CTCCCTTTCC TGGACAAGGC TTGTAGGAAG 
ATTGTTGACT TGAATTTTCA ATCTCGGTTA GATGAGGATA AAGCTCCTCC TTCCTCCCTT 
TTAGTTCATA AGCTTATTTT CCAGTACATT GAGGAAAAGG AATCCTGGAA GAAAAAATGT 
ACAACACAGC ATCAAATACC CAAGATGCTT CAAGAAATCT CATTGGTAGT GAGCCATTGT 
AGACTCCTTG GAGAGGAAAT TGAGTTTTTA AAGAGATGGG GACCAAATTA TAACCTAATG 
AACATAGATG TGACTAATAC TGAATTGAGG CTTTTATTCT CCAGCTCTGC AGCATTTGCA 
AAATTTGAGA TAACTTTGCC TCTCTCAGTC CATTTTCCAA CTGTTCCATT ACCTTTCTCC 
ATTCAAAAGC ACCTTGGAAA CATTGGCCAA GATGAAATCA CTGCAATTCT ATCTAAAGTG 
CCACTGAAGA ACAACTATCT AAAGAATGTA GTCAAGCAAA TTTACCAAGA TCTGCTTCAG 
GACTGCCATT TCTATCACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005638312.1
CDS	354..7214
Translation	MDGMSSETNEENDNMERPVRRRHSSILKPPRSPLQDLRGGNEAVQESNALRNKKSSRRVSFADTIKVFQTESHMKIVRKSEIAETEAGENVLYIQNKKPEETYCEITGMNTLLSAPIQTQMQQREISITEHNHEMKPANDQTVIFSDENQMDLTASHTVMITKDLLDCTKSEKSIKIDTASFLANLKLHTKDSRMKKELNFSMDQSTSSEKKIHFNDFIKRLKTGKSNAFPTGPDKENFEISVHPIKTKKASSVHQMHVSLRVDENSSNTTRIFGEQNDGMNFTQCHTASIQTLVPTSSKASIGEFKGDDITIYGSDFMDLTTNHTIQNLPSADNSSNKENQTQNVMMEVKAPGGKTIFNKLNIPFQTPLNPNGEIHITKSHITGTETHTSTQTSNQDARTLTIIPESICSSPATQDYKTFFYSSSNNAMELTTCLSGMRKEKNLLKNDNYSKMYPNPDAISLLTEKTIYSGEDSMDITRSHTVAIDNQIFKQDQTNIQKADTSISEKEIMLQNLITMSKDGKMNINCSSVPHISKGRLQQSLANSLSISLTDKKTEIFTGEDMDLTKSHTTNLGSQAPLTSYNVTSENTSKFLSHSKSPSGEWQEMTKSRIEPSQQPDIISKNIPVDTWGKEKVQVLEIAPYVDKDSPQSADINQDIAISHSIVYSDRNLEKQVALGNNRNTVPCEQSLFPTSESLFSLEGQSNMKNHNIAINSYTMKSVPGQNSKLPEPLRKGVGNPTPDYSHDKTIICSEEEQNMDLTKSHTVVIGFSPSEIQELGKTNLENTNSLLTTANRQTAIKVEKCCKSPIEKTGISVSKDNLDVIEDNSIQKLGFLNEKQDVKICGRKSVGRQKVDKTIVFSEGDENDMDITKIFTVEINHKPLLLEQDSHLAGTSKTVLYECGQDDVEITKSHTTALECITVSPDKITSRPMDKTVMFVDNYNELEMTKSHTVFIDYQAKERTVSIDKLDTELSKRRKSIEKPKMTPASAEESVSFLENGESDHSAANVNKLTLLREWSNNGPIEKAVAGDNMEVSKFTIWKNGKDVQKPEFLNDTISDKSQRRKSLRLKNDKIAAFSKNDDDMDITQSCTVKINYKSAPEDREDSHLVPLTGASKTVLYSCGQDDMEMTRSHTTALECKTVSPDKITTRPIDKTVMFVDNHGDLEVTESHTVFIDCQNTEKINQECPKFGIATGKPLGISFHNDGSSVQEITEKQALAVDQTYIPEKARIGSCQLNSTDRRNMDFTNSQATATCGSSDNYSCLPNAISCFDNLEGNVMSLSNKDKKASNCPVQNDLSYVKDLANEYYLKPEGLPPSACSLLKKEKVTQTNTNGQLDCVTTVLKDQDMIKESQNLIANQTLQDSQELAEMIKPNSNRVSFKPPKDQMEGFVDTTEHNLKKTVVDNVCVTSQPHLTSQVPPLPQQGRIILNKDETISSNSGNKNLNIVIGNSFVPTCENEAKMLNNDKQFTIVCKNELRKNTKCNTDFHSNSDLTKQVIQTHTNSREASDTVIASNTSFNSVRSNLNNLNGKTEEFLDFQTPHIPSSPEQILALINKAHNHMSILQATEIQNINPAPSNVEDDKDEESKISHNGAETNSVPLMTVVKDKVKRCSLGIFLPRLPNKRNCSVTGINDLKQIQGDTTDLNHLEIQPVFSKEPSIEFVATKLTLSPSQYINEENFPIYPGEINSSDSITIETEEKALIETYQKGIPPSENKIRETCSNQKRTWVQEDDDIQNEKKIRKSEIRCSDTTQDQEIFDHHAEGDIDKNANSLSRTPSSCSSSLDSIKADGTSLDFSTQHNSQMESQFLRDTICEESLKEKLKDGKITIKEFFILLQVHILIQKPRQSNLPAKFTINTPPTLEDLMLSQYVYRPKIQIYREDCEALCQKIEELKLSALNQDKLLTDINRNLWEKMRHCSDEELKVFGIYLNKIKSHFTKMTKVFTHQGKVALYTKLVQSAQNERKKLQIRIDEMDNILKKIENCLAEVEIETKNLDEEKDNPMEEWDSEMRAAEKELEQLKTEEDELQRNLLELEVQKEQTLAQIDFVQKQTDRTEELMDQLSLSEWEAIEWSDDQAVFTFLYDTIELTITFGETVAGSPKIRLPAWSVDLPFLDKACRKIVDLNFQSRLDEDKAPPSSLLVHKLIFQYIEEKESWKKKCTTQHQIPKMLQEISLVVSHCRLLGEEIEFLKRWGPNYNLMNIDVTNTELRLLFSSSAAFAKFEITLPLSVHFPTVPLPFSIQKHLGNIGQDEITAILSKVPLKNNYLKNVVKQIYQDLLQDCHFYH

Target ORF information:

RefSeq Version	XM_005638255.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris cancer susceptibility candidate 5 (CASC5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005638255.1

ORF Insert Sequence:

ATGGATGGGA TGTCTTCAGA GACTAATGAA GAGAATGACA ATATGGAGAG ACCTGTTAGA 
AGACGACATT CCTCCATTTT GAAACCCCCA AGGAGTCCTC TTCAGGACCT CAGAGGTGGG 
AATGAAGCAG TTCAGGAATC TAATGCTTTG AGAAATAAGA AAAGCTCTCG TCGAGTCAGC 
TTTGCAGATA CCATAAAAGT ATTCCAAACA GAGTCTCATA TGAAAATAGT AAGAAAGTCA 
GAAATTGCAG AAACAGAAGC AGGAGAAAAT GTTCTATATA TACAGAATAA GAAGCCAGAA 
GAAACCTACT GTGAAATTAC TGGGATGAAC ACATTGCTTT CTGCTCCTAT TCAGACTCAG 
ATGCAACAGA GAGAGATTTC AATTACAGAA CACAACCATG AAATGAAACC TGCAAATGAC 
CAAACAGTCA TCTTTTCAGA TGAAAACCAG ATGGACCTAA CAGCAAGTCA CACCGTAATG 
ATTACCAAAG ACCTGTTAGA TTGTACTAAA AGTGAAAAGT CCATTAAGAT AGATACCGCA 
TCATTTCTGG CTAACTTAAA GCTCCATACT AAGGATTCAA GAATGAAAAA AGAATTAAAT 
TTTTCCATGG ATCAAAGCAC TTCTTCAGAA AAGAAAATAC ATTTTAATGA CTTCATAAAA 
AGATTGAAAA CAGGAAAATC TAATGCTTTT CCTACAGGAC CTGATAAAGA AAATTTTGAA 
ATATCTGTTC ATCCCATAAA AACAAAGAAG GCCTCTTCTG TACATCAAAT GCATGTATCT 
CTTAGGGTAG ATGAAAATAG TAGTAATACA ACTAGAATCT TCGGAGAACA AAATGATGGG 
ATGAATTTCA CTCAGTGTCA TACAGCCAGT ATTCAGACTT TGGTTCCCAC ATCCAGTAAG 
GCCAGCATAG GGGAATTTAA AGGTGATGAT ATTACAATTT ATGGCAGTGA TTTTATGGAC 
TTGACAACTA ACCACACTAT ACAGAATTTA CCCTCAGCAG ATAATTCATC TAATAAAGAA 
AATCAAACTC AGAATGTCAT GATGGAGGTT AAAGCTCCAG GAGGGAAGAC AATCTTTAAT 
AAACTGAATA TTCCTTTTCA AACCCCTTTA AACCCTAATG GTGAAATACA TATTACCAAA 
AGTCATATTA CAGGGACAGA AACTCACACA AGCACACAGA CTTCTAACCA AGATGCCAGA 
ACATTGACTA TAATCCCAGA ATCTATATGT TCCAGTCCAG CTACTCAAGA TTATAAGACA 
TTTTTCTATT CTAGCAGTAA TAATGCCATG GAACTGACCA CGTGTCTCTC GGGTATGAGA 
AAAGAGAAGA ATTTGCTGAA GAATGATAAT TATTCTAAAA TGTATCCCAA TCCAGATGCC 
ATCTCTCTTC TTACAGAGAA AACTATTTAT TCAGGAGAGG ATAGCATGGA CATTACGAGG 
AGTCATACAG TTGCAATAGA TAATCAGATT TTTAAACAAG ATCAGACAAA TATACAGAAA 
GCAGACACAT CAATATCTGA AAAAGAAATA ATGCTCCAAA ATCTCATAAC CATGTCAAAG 
GACGGGAAAA TGAATATAAA TTGTAGCTCA GTTCCTCATA TATCTAAGGG AAGATTACAG 
CAGAGCCTGG CAAACTCTTT ATCTATTTCA TTGACTGACA AAAAGACTGA AATCTTCACA 
GGTGAAGATA TGGATTTGAC TAAAAGTCAC ACAACTAACT TAGGAAGTCA AGCTCCACTT 
ACATCTTACA ATGTAACATC TGAGAATACC AGTAAATTTC TCTCTCACAG CAAAAGCCCT 
TCAGGTGAAT GGCAAGAAAT GACCAAAAGT CGTATTGAAC CATCCCAGCA GCCAGACATA 
ATATCTAAAA ACATCCCAGT GGACACCTGG GGCAAAGAAA AAGTTCAAGT TTTAGAGATT 
GCACCTTATG TTGATAAAGA TTCTCCTCAA TCAGCTGATA TTAATCAAGA CATTGCAATA 
AGCCATAGTA TAGTATACTC TGATAGAAAT CTTGAGAAAC AAGTAGCACT AGGAAATAAT 
AGAAATACTG TTCCATGTGA GCAATCTTTG TTTCCTACTT CAGAGTCATT ATTTTCATTA 
GAAGGACAGT CTAACATGAA AAATCATAAT ATTGCTATTA ACAGTTACAC AATGAAATCT 
GTGCCAGGCC AGAATTCTAA ACTGCCTGAG CCACTGAGGA AAGGTGTAGG CAATCCAACA 
CCTGACTATT CTCATGACAA AACAATTATT TGTTCAGAGG AGGAACAAAA TATGGATCTA 
ACCAAGAGTC ACACTGTTGT CATTGGATTT AGTCCTTCTG AAATACAAGA ATTGGGTAAG 
ACTAATCTAG AAAATACTAA CAGTCTGCTA ACAACAGCAA ACAGACAGAC AGCTATAAAA 
GTTGAAAAAT GTTGTAAAAG TCCTATAGAA AAAACAGGAA TATCTGTTTC TAAGGATAAC 
CTAGATGTGA TAGAGGACAA TAGCATACAG AAACTTGGAT TTTTGAATGA AAAGCAAGAT 
GTCAAAATTT GTGGAAGAAA AAGTGTTGGC AGACAAAAAG TTGATAAGAC TATTGTATTT 
TCAGAGGGTG ATGAGAATGA TATGGATATC ACCAAGATTT TTACAGTGGA AATAAATCAT 
AAACCTTTAT TACTTGAACA AGATTCCCAT TTGGCAGGAA CTTCTAAAAC TGTTTTGTAT 
GAATGTGGGC AAGATGATGT GGAGATCACT AAAAGTCACA CAACTGCCTT GGAATGTATA 
ACTGTCTCAC CAGATAAAAT AACCAGTAGG CCTATGGACA AAACTGTAAT GTTTGTAGAT 
AATTACAATG AACTGGAAAT GACAAAGTCT CACACTGTTT TCATTGACTA CCAAGCAAAG 
GAGAGGACTG TGAGTATAGA CAAACTTGAC ACGGAACTAT CCAAAAGAAG AAAAAGCATA 
GAAAAGCCAA AAATGACACC TGCTTCTGCT GAGGAGAGTG TTTCCTTTCT GGAGAATGGT 
GAAAGTGACC ATTCAGCAGC AAACGTCAAT AAGCTAACAC TACTGAGGGA ATGGTCTAAT 
AATGGTCCTA TAGAGAAAGC AGTAGCTGGT GATAACATGG AAGTATCTAA ATTCACCATT 
TGGAAAAATG GCAAAGATGT ACAAAAGCCT GAATTTCTGA ATGACACTAT ATCAGACAAA 
AGTCAGAGAA GGAAAAGCCT TAGACTCAAA AATGACAAGA TTGCTGCATT TTCTAAAAAT 
GATGATGATA TGGATATCAC CCAGAGTTGT ACAGTGAAAA TAAACTATAA AAGTGCCCCA 
GAAGATAGAG AGGATTCCCA TTTGGTGCCT TTGACAGGAG CTTCTAAAAC TGTTTTGTAT 
TCATGTGGGC AGGATGACAT GGAGATGACT AGAAGTCATA CGACTGCGTT AGAATGTAAA 
ACTGTCTCAC CGGATAAAAT CACTACTAGG CCCATAGATA AAACTGTAAT GTTTGTTGAT 
AATCATGGTG ATCTGGAAGT CACCGAGTCC CATACTGTTT TCATTGATTG TCAAAACACA 
GAGAAAATTA ATCAAGAGTG CCCTAAATTT GGAATAGCTA CAGGAAAACC CTTGGGTATT 
TCTTTTCATA ATGATGGTAG CTCTGTTCAA GAAATCACTG AAAAACAAGC ATTGGCAGTA 
GACCAGACCT ATATACCGGA AAAAGCCAGA ATTGGAAGCT GTCAGTTAAA TAGTACAGAT 
AGAAGAAACA TGGACTTTAC TAACAGTCAA GCAACTGCTA CGTGTGGATC CAGTGATAAT 
TATTCCTGTT TACCAAATGC TATTTCCTGT TTTGATAATT TAGAGGGGAA TGTCATGTCC 
TTAAGTAATA AAGATAAGAA AGCCAGTAAC TGCCCAGTGC AAAATGATCT TTCTTATGTA 
AAGGATTTAG CCAATGAATA TTACTTGAAA CCTGAGGGAC TGCCTCCCTC TGCTTGTTCT 
TTGTTAAAGA AAGAAAAAGT GACTCAAACC AATACCAATG GACAGTTAGA CTGTGTTACA 
ACAGTGCTTA AAGATCAAGA TATGATTAAG GAGTCCCAGA ATCTAATAGC TAATCAAACT 
TTACAAGATA GTCAGGAGCT GGCGGAGATG ATTAAACCTA ATTCAAATCG AGTATCCTTT 
AAACCCCCAA AGGATCAAAT GGAGGGCTTT GTTGATACCA CGGAACATAA TTTAAAGAAG 
ACTGTTGTTG ATAATGTTTG TGTTACTTCT CAGCCTCATC TCACAAGCCA GGTACCTCCA 
TTACCTCAAC AAGGACGGAT AATTTTGAAT AAAGATGAAA CAATATCATC TAATTCTGGA 
AATAAGAATT TAAATATTGT TATAGGAAAT TCCTTTGTAC CCACATGTGA AAACGAGGCC 
AAAATGCTCA ACAATGATAA ACAATTTACC ATAGTCTGTA AAAATGAGCT GAGGAAAAAT 
ACTAAATGTA ATACCGATTT CCACAGTAAT TCAGACTTGA CTAAGCAAGT TATTCAAACT 
CATACTAATT CTAGAGAAGC ATCAGATACT GTAATTGCAT CTAATACAAG TTTTAATAGT 
GTCAGATCAA ATCTGAATAA TTTGAATGGA AAAACTGAAG AATTTTTAGA TTTCCAAACT 
CCCCATATTC CATCTTCTCC AGAGCAAATA CTTGCATTAA TAAACAAGGC ACACAATCAT 
ATGAGTATAT TGCAAGCTAC AGAAATACAG AACATTAACC CAGCGCCCAG CAATGTTGAA 
GATGATAAAG ATGAAGAAAG CAAAATTTCT CATAATGGAG CTGAAACCAA CTCTGTACCA 
CTCATGACAG TTGTAAAAGA TAAAGTGAAG AGATGTTCTT TGGGAATATT TTTGCCCAGA 
TTGCCAAACA AGAGAAATTG TAGTGTCACT GGTATTAATG ACCTGAAGCA GATTCAAGGA 
GACACAACTG ATTTAAATCA CTTAGAAATT CAGCCAGTCT TCAGTAAGGA GCCAAGCATT 
GAATTTGTTG CAACTAAACT GACCTTAAGT CCATCTCAGT ATATAAATGA GGAAAACTTT 
CCTATATATC CTGGTGAGAT TAATTCCTCA GATTCTATTA CCATAGAAAC TGAGGAAAAG 
GCTTTGATTG AGACATATCA AAAAGGGATT CCACCATCTG AAAATAAGAT AAGAGAAACC 
TGCAGTAACC AAAAAAGAAC CTGGGTACAA GAAGATGATG ATATTCAGAA TGAGAAAAAA 
ATCAGGAAAA GTGAGATTAG GTGTAGTGAT ACTACACAAG ATCAGGAGAT TTTTGATCAC 
CATGCTGAAG GGGATATAGA TAAAAATGCT AACAGCCTGA GCAGAACCCC ATCTAGTTGC 
AGCAGTTCTC TGGATTCGAT AAAGGCTGAT GGGACCTCTC TGGACTTCAG CACACAGCAC 
AATAGTCAAA TGGAATCACA GTTTCTCAGA GACACTATTT GTGAAGAGAG CTTGAAGGAG 
AAACTCAAAG ATGGGAAAAT TACAATAAAG GAGTTCTTTA TACTTCTTCA GGTCCACATT 
TTGATACAGA AACCCCGACA GAGCAATCTC CCAGCCAAAT TTACTATAAA CACACCACCT 
ACTCTGGAAG ACCTGATGTT AAGTCAGTAT GTTTACCGAC CCAAGATACA GATTTATAGA 
GAAGATTGTG AAGCTCTTTG CCAAAAGATT GAAGAATTAA AGCTTTCTGC ATTGAACCAG 
GATAAATTGT TGACTGACAT AAATAGGAAC CTGTGGGAAA AAATGAGACA CTGCTCTGAT 
GAAGAGCTGA AGGTCTTTGG AATTTACCTG AACAAAATAA AATCACATTT TACCAAGATG 
ACTAAAGTTT TTACTCACCA AGGAAAAGTG GCTCTCTATA CCAAGCTGGT ACAGTCAGCT 
CAGAATGAGA GGAAGAAACT TCAGATAAGG ATAGATGAGA TGGACAACAT ACTTAAGAAG 
ATTGAGAACT GTCTCGCTGA AGTTGAAATA GAAACTAAGA ATTTGGATGA AGAGAAAGAC 
AATCCTATGG AAGAATGGGA TTCTGAAATG AGAGCCGCAG AGAAAGAACT AGAACAGCTG 
AAAACTGAAG AAGATGAGCT CCAAAGAAAT CTCTTAGAAC TGGAAGTACA GAAAGAGCAG 
ACCCTTGCTC AAATAGACTT TGTACAAAAA CAAACAGATA GAACTGAAGA GCTAATGGAT 
CAGTTGAGCT TGTCTGAGTG GGAAGCCATT GAGTGGAGTG ATGATCAAGC TGTATTCACC 
TTTCTTTATG ACACAATAGA ACTTACTATC ACCTTTGGAG AGACAGTAGC TGGAAGTCCC 
AAGATCAGGC TGCCAGCATG GTCAGTTGAT CTCCCTTTCC TGGACAAGGC TTGTAGGAAG 
ATTGTTGACT TGAATTTTCA ATCTCGGTTA GATGAGGATA AAGCTCCTCC TTCCTCCCTT 
TTAGTTCATA AGCTTATTTT CCAGTACATT GAGGAAAAGG AATCCTGGAA GAAAAAATGT 
ACAACACAGC ATCAAATACC CAAGATGCTT CAAGAAATCT CATTGGTAGT GAGCCATTGT 
AGACTCCTTG GAGAGGAAAT TGAGTTTTTA AAGAGATGGG GACCAAATTA TAACCTAATG 
AACATAGATG TGACTAATAC TGAATTGAGG CTTTTATTCT CCAGCTCTGC AGCATTTGCA 
AAATTTGAGA TAACTTTGCC TCTCTCAGTC CATTTTCCAA CTGTTCCATT ACCTTTCTCC 
ATTCAAAAGC ACCTTGGAAA CATTGGCCAA GATGAAATCA CTGCAATTCT ATCTAAAGTG 
CCACTGAAGA ACAACTATCT AAAGAATGTA GTCAAGCAAA TTTACCAAGA TCTGCTTCAG 
GACTGCCATT TCTATCACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KNL1 ( XM_014109668.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013965143.1 Canis lupus familiaris cancer susceptibility candidate 5 (CASC5), transcript variant X2, mRNA.

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CloneID	OCe28661
Clone ID Related Accession (Same CDS sequence)	XM_014109669.1 , XM_005638255.2 , XM_005638255.1 , XM_014109668.1 , XM_014109668.2
Accession Version	XM_014109668.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6861bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	protein CASC5 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726113.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGGGA TGTCTTCAGA GACTAATGAA GAGAATGACA ATATGGAGAG ACCTGTTAGA 
AGACGACATT CCTCCATTTT GAAACCCCCA AGGAGTCCTC TTCAGGACCT CAGAGGTGGG 
AATGAAGCAG TTCAGGAATC TAATGCTTTG AGAAATAAGA AAAGCTCTCG TCGAGTCAGC 
TTTGCAGATA CCATAAAAGT ATTCCAAACA GAGTCTCATA TGAAAATAGT AAGAAAGTCA 
GAAATTGCAG AAACAGAAGC AGGAGAAAAT GTTCTATATA TACAGAATAA GAAGCCAGAA 
GAAACCTACT GTGAAATTAC TGGGATGAAC ACATTGCTTT CTGCTCCTAT TCAGACTCAG 
ATGCAACAGA GAGAGATTTC AATTACAGAA CACAACCATG AAATGAAACC TGCAAATGAC 
CAAACAGTCA TCTTTTCAGA TGAAAACCAG ATGGACCTAA CAGCAAGTCA CACCGTAATG 
ATTACCAAAG ACCTGTTAGA TTGTACTAAA AGTGAAAAGT CCATTAAGAT AGATACCGCA 
TCATTTCTGG CTAACTTAAA GCTCCATACT AAGGATTCAA GAATGAAAAA AGAATTAAAT 
TTTTCCATGG ATCAAAGCAC TTCTTCAGAA AAGAAAATAC ATTTTAATGA CTTCATAAAA 
AGATTGAAAA CAGGAAAATC TAATGCTTTT CCTACAGGAC CTGATAAAGA AAATTTTGAA 
ATATCTGTTC ATCCCATAAA AACAAAGAAG GCCTCTTCTG TACATCAAAT GCATGTATCT 
CTTAGGGTAG ATGAAAATAG TAGTAATACA ACTAGAATCT TCGGAGAACA AAATGATGGG 
ATGAATTTCA CTCAGTGTCA TACAGCCAGT ATTCAGACTT TGGTTCCCAC ATCCAGTAAG 
GCCAGCATAG GGGAATTTAA AGGTGATGAT ATTACAATTT ATGGCAGTGA TTTTATGGAC 
TTGACAACTA ACCACACTAT ACAGAATTTA CCCTCAGCAG ATAATTCATC TAATAAAGAA 
AATCAAACTC AGAATGTCAT GATGGAGGTT AAAGCTCCAG GAGGGAAGAC AATCTTTAAT 
AAACTGAATA TTCCTTTTCA AACCCCTTTA AACCCTAATG GTGAAATACA TATTACCAAA 
AGTCATATTA CAGGGACAGA AACTCACACA AGCACACAGA CTTCTAACCA AGATGCCAGA 
ACATTGACTA TAATCCCAGA ATCTATATGT TCCAGTCCAG CTACTCAAGA TTATAAGACA 
TTTTTCTATT CTAGCAGTAA TAATGCCATG GAACTGACCA CGTGTCTCTC GGGTATGAGA 
AAAGAGAAGA ATTTGCTGAA GAATGATAAT TATTCTAAAA TGTATCCCAA TCCAGATGCC 
ATCTCTCTTC TTACAGAGAA AACTATTTAT TCAGGAGAGG ATAGCATGGA CATTACGAGG 
AGTCATACAG TTGCAATAGA TAATCAGATT TTTAAACAAG ATCAGACAAA TATACAGAAA 
GCAGACACAT CAATATCTGA AAAAGAAATA ATGCTCCAAA ATCTCATAAC CATGTCAAAG 
GACGGGAAAA TGAATATAAA TTGTAGCTCA GTTCCTCATA TATCTAAGGG AAGATTACAG 
CAGAGCCTGG CAAACTCTTT ATCTATTTCA TTGACTGACA AAAAGACTGA AATCTTCACA 
GGTGAAGATA TGGATTTGAC TAAAAGTCAC ACAACTAACT TAGGAAGTCA AGCTCCACTT 
ACATCTTACA ATGTAACATC TGAGAATACC AGTAAATTTC TCTCTCACAG CAAAAGCCCT 
TCAGGTGAAT GGCAAGAAAT GACCAAAAGT CGTATTGAAC CATCCCAGCA GCCAGACATA 
ATATCTAAAA ACATCCCAGT GGACACCTGG GGCAAAGAAA AAGTTCAAGT TTTAGAGATT 
GCACCTTATG TTGATAAAGA TTCTCCTCAA TCAGCTGATA TTAATCAAGA CATTGCAATA 
AGCCATAGTA TAGTATACTC TGATAGAAAT CTTGAGAAAC AAGTAGCACT AGGAAATAAT 
AGAAATACTG TTCCATGTGA GCAATCTTTG TTTCCTACTT CAGAGTCATT ATTTTCATTA 
GAAGGACAGT CTAACATGAA AAATCATAAT ATTGCTATTA ACAGTTACAC AATGAAATCT 
GTGCCAGGCC AGAATTCTAA ACTGCCTGAG CCACTGAGGA AAGGTGTAGG CAATCCAACA 
CCTGACTATT CTCATGACAA AACAATTATT TGTTCAGAGG AGGAACAAAA TATGGATCTA 
ACCAAGAGTC ACACTGTTGT CATTGGATTT AGTCCTTCTG AAATACAAGA ATTGGGTAAG 
ACTAATCTAG AAAATACTAA CAGTCTGCTA ACAACAGCAA ACAGACAGAC AGCTATAAAA 
GTTGAAAAAT GTTGTAAAAG TCCTATAGAA AAAACAGGAA TATCTGTTTC TAAGGATAAC 
CTAGATGTGA TAGAGGACAA TAGCATACAG AAACTTGGAT TTTTGAATGA AAAGCAAGAT 
GTCAAAATTT GTGGAAGAAA AAGTGTTGGC AGACAAAAAG TTGATAAGAC TATTGTATTT 
TCAGAGGGTG ATGAGAATGA TATGGATATC ACCAAGATTT TTACAGTGGA AATAAATCAT 
AAACCTTTAT TACTTGAACA AGATTCCCAT TTGGCAGGAA CTTCTAAAAC TGTTTTGTAT 
GAATGTGGGC AAGATGATGT GGAGATCACT AAAAGTCACA CAACTGCCTT GGAATGTATA 
ACTGTCTCAC CAGATAAAAT AACCAGTAGG CCTATGGACA AAACTGTAAT GTTTGTAGAT 
AATTACAATG AACTGGAAAT GACAAAGTCT CACACTGTTT TCATTGACTA CCAAGCAAAG 
GAGAGGACTG TGAGTATAGA CAAACTTGAC ACGGAACTAT CCAAAAGAAG AAAAAGCATA 
GAAAAGCCAA AAATGACACC TGCTTCTGCT GAGGAGAGTG TTTCCTTTCT GGAGAATGGT 
GAAAGTGACC ATTCAGCAGC AAACGTCAAT AAGCTAACAC TACTGAGGGA ATGGTCTAAT 
AATGGTCCTA TAGAGAAAGC AGTAGCTGGT GATAACATGG AAGTATCTAA ATTCACCATT 
TGGAAAAATG GCAAAGATGT ACAAAAGCCT GAATTTCTGA ATGACACTAT ATCAGACAAA 
AGTCAGAGAA GGAAAAGCCT TAGACTCAAA AATGACAAGA TTGCTGCATT TTCTAAAAAT 
GATGATGATA TGGATATCAC CCAGAGTTGT ACAGTGAAAA TAAACTATAA AAGTGCCCCA 
GAAGATAGAG AGGATTCCCA TTTGGTGCCT TTGACAGGAG CTTCTAAAAC TGTTTTGTAT 
TCATGTGGGC AGGATGACAT GGAGATGACT AGAAGTCATA CGACTGCGTT AGAATGTAAA 
ACTGTCTCAC CGGATAAAAT CACTACTAGG CCCATAGATA AAACTGTAAT GTTTGTTGAT 
AATCATGGTG ATCTGGAAGT CACCGAGTCC CATACTGTTT TCATTGATTG TCAAAACACA 
GAGAAAATTA ATCAAGAGTG CCCTAAATTT GGAATAGCTA CAGGAAAACC CTTGGGTATT 
TCTTTTCATA ATGATGGTAG CTCTGTTCAA GAAATCACTG AAAAACAAGC ATTGGCAGTA 
GACCAGACCT ATATACCGGA AAAAGCCAGA ATTGGAAGCT GTCAGTTAAA TAGTACAGAT 
AGAAGAAACA TGGACTTTAC TAACAGTCAA GCAACTGCTA CGTGTGGATC CAGTGATAAT 
TATTCCTGTT TACCAAATGC TATTTCCTGT TTTGATAATT TAGAGGGGAA TGTCATGTCC 
TTAAGTAATA AAGATAAGAA AGCCAGTAAC TGCCCAGTGC AAAATGATCT TTCTTATGTA 
AAGGATTTAG CCAATGAATA TTACTTGAAA CCTGAGGGAC TGCCTCCCTC TGCTTGTTCT 
TTGTTAAAGA AAGAAAAAGT GACTCAAACC AATACCAATG GACAGTTAGA CTGTGTTACA 
ACAGTGCTTA AAGATCAAGA TATGATTAAG GAGTCCCAGA ATCTAATAGC TAATCAAACT 
TTACAAGATA GTCAGGAGCT GGCGGAGATG ATTAAACCTA ATTCAAATCG AGTATCCTTT 
AAACCCCCAA AGGATCAAAT GGAGGGCTTT GTTGATACCA CGGAACATAA TTTAAAGAAG 
ACTGTTGTTG ATAATGTTTG TGTTACTTCT CAGCCTCATC TCACAAGCCA GGTACCTCCA 
TTACCTCAAC AAGGACGGAT AATTTTGAAT AAAGATGAAA CAATATCATC TAATTCTGGA 
AATAAGAATT TAAATATTGT TATAGGAAAT TCCTTTGTAC CCACATGTGA AAACGAGGCC 
AAAATGCTCA ACAATGATAA ACAATTTACC ATAGTCTGTA AAAATGAGCT GAGGAAAAAT 
ACTAAATGTA ATACCGATTT CCACAGTAAT TCAGACTTGA CTAAGCAAGT TATTCAAACT 
CATACTAATT CTAGAGAAGC ATCAGATACT GTAATTGCAT CTAATACAAG TTTTAATAGT 
GTCAGATCAA ATCTGAATAA TTTGAATGGA AAAACTGAAG AATTTTTAGA TTTCCAAACT 
CCCCATATTC CATCTTCTCC AGAGCAAATA CTTGCATTAA TAAACAAGGC ACACAATCAT 
ATGAGTATAT TGCAAGCTAC AGAAATACAG AACATTAACC CAGCGCCCAG CAATGTTGAA 
GATGATAAAG ATGAAGAAAG CAAAATTTCT CATAATGGAG CTGAAACCAA CTCTGTACCA 
CTCATGACAG TTGTAAAAGA TAAAGTGAAG AGATGTTCTT TGGGAATATT TTTGCCCAGA 
TTGCCAAACA AGAGAAATTG TAGTGTCACT GGTATTAATG ACCTGAAGCA GATTCAAGGA 
GACACAACTG ATTTAAATCA CTTAGAAATT CAGCCAGTCT TCAGTAAGGA GCCAAGCATT 
GAATTTGTTG CAACTAAACT GACCTTAAGT CCATCTCAGT ATATAAATGA GGAAAACTTT 
CCTATATATC CTGGTGAGAT TAATTCCTCA GATTCTATTA CCATAGAAAC TGAGGAAAAG 
GCTTTGATTG AGACATATCA AAAAGGGATT CCACCATCTG AAAATAAGAT AAGAGAAACC 
TGCAGTAACC AAAAAAGAAC CTGGGTACAA GAAGATGATG ATATTCAGAA TGAGAAAAAA 
ATCAGGAAAA GTGAGATTAG GTGTAGTGAT ACTACACAAG ATCAGGAGAT TTTTGATCAC 
CATGCTGAAG GGGATATAGA TAAAAATGCT AACAGCCTGA GCAGAACCCC ATCTAGTTGC 
AGCAGTTCTC TGGATTCGAT AAAGGCTGAT GGGACCTCTC TGGACTTCAG CACACAGCAC 
AATAGTCAAA TGGAATCACA GTTTCTCAGA GACACTATTT GTGAAGAGAG CTTGAAGGAG 
AAACTCAAAG ATGGGAAAAT TACAATAAAG GAGTTCTTTA TACTTCTTCA GGTCCACATT 
TTGATACAGA AACCCCGACA GAGCAATCTC CCAGCCAAAT TTACTATAAA CACACCACCT 
ACTCTGGAAG ACCTGATGTT AAGTCAGTAT GTTTACCGAC CCAAGATACA GATTTATAGA 
GAAGATTGTG AAGCTCTTTG CCAAAAGATT GAAGAATTAA AGCTTTCTGC ATTGAACCAG 
GATAAATTGT TGACTGACAT AAATAGGAAC CTGTGGGAAA AAATGAGACA CTGCTCTGAT 
GAAGAGCTGA AGGTCTTTGG AATTTACCTG AACAAAATAA AATCACATTT TACCAAGATG 
ACTAAAGTTT TTACTCACCA AGGAAAAGTG GCTCTCTATA CCAAGCTGGT ACAGTCAGCT 
CAGAATGAGA GGAAGAAACT TCAGATAAGG ATAGATGAGA TGGACAACAT ACTTAAGAAG 
ATTGAGAACT GTCTCGCTGA AGTTGAAATA GAAACTAAGA ATTTGGATGA AGAGAAAGAC 
AATCCTATGG AAGAATGGGA TTCTGAAATG AGAGCCGCAG AGAAAGAACT AGAACAGCTG 
AAAACTGAAG AAGATGAGCT CCAAAGAAAT CTCTTAGAAC TGGAAGTACA GAAAGAGCAG 
ACCCTTGCTC AAATAGACTT TGTACAAAAA CAAACAGATA GAACTGAAGA GCTAATGGAT 
CAGTTGAGCT TGTCTGAGTG GGAAGCCATT GAGTGGAGTG ATGATCAAGC TGTATTCACC 
TTTCTTTATG ACACAATAGA ACTTACTATC ACCTTTGGAG AGACAGTAGC TGGAAGTCCC 
AAGATCAGGC TGCCAGCATG GTCAGTTGAT CTCCCTTTCC TGGACAAGGC TTGTAGGAAG 
ATTGTTGACT TGAATTTTCA ATCTCGGTTA GATGAGGATA AAGCTCCTCC TTCCTCCCTT 
TTAGTTCATA AGCTTATTTT CCAGTACATT GAGGAAAAGG AATCCTGGAA GAAAAAATGT 
ACAACACAGC ATCAAATACC CAAGATGCTT CAAGAAATCT CATTGGTAGT GAGCCATTGT 
AGACTCCTTG GAGAGGAAAT TGAGTTTTTA AAGAGATGGG GACCAAATTA TAACCTAATG 
AACATAGATG TGACTAATAC TGAATTGAGG CTTTTATTCT CCAGCTCTGC AGCATTTGCA 
AAATTTGAGA TAACTTTGCC TCTCTCAGTC CATTTTCCAA CTGTTCCATT ACCTTTCTCC 
ATTCAAAAGC ACCTTGGAAA CATTGGCCAA GATGAAATCA CTGCAATTCT ATCTAAAGTG 
CCACTGAAGA ACAACTATCT AAAGAATGTA GTCAAGCAAA TTTACCAAGA TCTGCTTCAG 
GACTGCCATT TCTATCACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013965143.1
CDS	316..7176
Translation	MDGMSSETNEENDNMERPVRRRHSSILKPPRSPLQDLRGGNEAVQESNALRNKKSSRRVSFADTIKVFQTESHMKIVRKSEIAETEAGENVLYIQNKKPEETYCEITGMNTLLSAPIQTQMQQREISITEHNHEMKPANDQTVIFSDENQMDLTASHTVMITKDLLDCTKSEKSIKIDTASFLANLKLHTKDSRMKKELNFSMDQSTSSEKKIHFNDFIKRLKTGKSNAFPTGPDKENFEISVHPIKTKKASSVHQMHVSLRVDENSSNTTRIFGEQNDGMNFTQCHTASIQTLVPTSSKASIGEFKGDDITIYGSDFMDLTTNHTIQNLPSADNSSNKENQTQNVMMEVKAPGGKTIFNKLNIPFQTPLNPNGEIHITKSHITGTETHTSTQTSNQDARTLTIIPESICSSPATQDYKTFFYSSSNNAMELTTCLSGMRKEKNLLKNDNYSKMYPNPDAISLLTEKTIYSGEDSMDITRSHTVAIDNQIFKQDQTNIQKADTSISEKEIMLQNLITMSKDGKMNINCSSVPHISKGRLQQSLANSLSISLTDKKTEIFTGEDMDLTKSHTTNLGSQAPLTSYNVTSENTSKFLSHSKSPSGEWQEMTKSRIEPSQQPDIISKNIPVDTWGKEKVQVLEIAPYVDKDSPQSADINQDIAISHSIVYSDRNLEKQVALGNNRNTVPCEQSLFPTSESLFSLEGQSNMKNHNIAINSYTMKSVPGQNSKLPEPLRKGVGNPTPDYSHDKTIICSEEEQNMDLTKSHTVVIGFSPSEIQELGKTNLENTNSLLTTANRQTAIKVEKCCKSPIEKTGISVSKDNLDVIEDNSIQKLGFLNEKQDVKICGRKSVGRQKVDKTIVFSEGDENDMDITKIFTVEINHKPLLLEQDSHLAGTSKTVLYECGQDDVEITKSHTTALECITVSPDKITSRPMDKTVMFVDNYNELEMTKSHTVFIDYQAKERTVSIDKLDTELSKRRKSIEKPKMTPASAEESVSFLENGESDHSAANVNKLTLLREWSNNGPIEKAVAGDNMEVSKFTIWKNGKDVQKPEFLNDTISDKSQRRKSLRLKNDKIAAFSKNDDDMDITQSCTVKINYKSAPEDREDSHLVPLTGASKTVLYSCGQDDMEMTRSHTTALECKTVSPDKITTRPIDKTVMFVDNHGDLEVTESHTVFIDCQNTEKINQECPKFGIATGKPLGISFHNDGSSVQEITEKQALAVDQTYIPEKARIGSCQLNSTDRRNMDFTNSQATATCGSSDNYSCLPNAISCFDNLEGNVMSLSNKDKKASNCPVQNDLSYVKDLANEYYLKPEGLPPSACSLLKKEKVTQTNTNGQLDCVTTVLKDQDMIKESQNLIANQTLQDSQELAEMIKPNSNRVSFKPPKDQMEGFVDTTEHNLKKTVVDNVCVTSQPHLTSQVPPLPQQGRIILNKDETISSNSGNKNLNIVIGNSFVPTCENEAKMLNNDKQFTIVCKNELRKNTKCNTDFHSNSDLTKQVIQTHTNSREASDTVIASNTSFNSVRSNLNNLNGKTEEFLDFQTPHIPSSPEQILALINKAHNHMSILQATEIQNINPAPSNVEDDKDEESKISHNGAETNSVPLMTVVKDKVKRCSLGIFLPRLPNKRNCSVTGINDLKQIQGDTTDLNHLEIQPVFSKEPSIEFVATKLTLSPSQYINEENFPIYPGEINSSDSITIETEEKALIETYQKGIPPSENKIRETCSNQKRTWVQEDDDIQNEKKIRKSEIRCSDTTQDQEIFDHHAEGDIDKNANSLSRTPSSCSSSLDSIKADGTSLDFSTQHNSQMESQFLRDTICEESLKEKLKDGKITIKEFFILLQVHILIQKPRQSNLPAKFTINTPPTLEDLMLSQYVYRPKIQIYREDCEALCQKIEELKLSALNQDKLLTDINRNLWEKMRHCSDEELKVFGIYLNKIKSHFTKMTKVFTHQGKVALYTKLVQSAQNERKKLQIRIDEMDNILKKIENCLAEVEIETKNLDEEKDNPMEEWDSEMRAAEKELEQLKTEEDELQRNLLELEVQKEQTLAQIDFVQKQTDRTEELMDQLSLSEWEAIEWSDDQAVFTFLYDTIELTITFGETVAGSPKIRLPAWSVDLPFLDKACRKIVDLNFQSRLDEDKAPPSSLLVHKLIFQYIEEKESWKKKCTTQHQIPKMLQEISLVVSHCRLLGEEIEFLKRWGPNYNLMNIDVTNTELRLLFSSSAAFAKFEITLPLSVHFPTVPLPFSIQKHLGNIGQDEITAILSKVPLKNNYLKNVVKQIYQDLLQDCHFYH

Target ORF information:

RefSeq Version	XM_014109668.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris cancer susceptibility candidate 5 (CASC5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014109668.1

ORF Insert Sequence:

ATGGATGGGA TGTCTTCAGA GACTAATGAA GAGAATGACA ATATGGAGAG ACCTGTTAGA 
AGACGACATT CCTCCATTTT GAAACCCCCA AGGAGTCCTC TTCAGGACCT CAGAGGTGGG 
AATGAAGCAG TTCAGGAATC TAATGCTTTG AGAAATAAGA AAAGCTCTCG TCGAGTCAGC 
TTTGCAGATA CCATAAAAGT ATTCCAAACA GAGTCTCATA TGAAAATAGT AAGAAAGTCA 
GAAATTGCAG AAACAGAAGC AGGAGAAAAT GTTCTATATA TACAGAATAA GAAGCCAGAA 
GAAACCTACT GTGAAATTAC TGGGATGAAC ACATTGCTTT CTGCTCCTAT TCAGACTCAG 
ATGCAACAGA GAGAGATTTC AATTACAGAA CACAACCATG AAATGAAACC TGCAAATGAC 
CAAACAGTCA TCTTTTCAGA TGAAAACCAG ATGGACCTAA CAGCAAGTCA CACCGTAATG 
ATTACCAAAG ACCTGTTAGA TTGTACTAAA AGTGAAAAGT CCATTAAGAT AGATACCGCA 
TCATTTCTGG CTAACTTAAA GCTCCATACT AAGGATTCAA GAATGAAAAA AGAATTAAAT 
TTTTCCATGG ATCAAAGCAC TTCTTCAGAA AAGAAAATAC ATTTTAATGA CTTCATAAAA 
AGATTGAAAA CAGGAAAATC TAATGCTTTT CCTACAGGAC CTGATAAAGA AAATTTTGAA 
ATATCTGTTC ATCCCATAAA AACAAAGAAG GCCTCTTCTG TACATCAAAT GCATGTATCT 
CTTAGGGTAG ATGAAAATAG TAGTAATACA ACTAGAATCT TCGGAGAACA AAATGATGGG 
ATGAATTTCA CTCAGTGTCA TACAGCCAGT ATTCAGACTT TGGTTCCCAC ATCCAGTAAG 
GCCAGCATAG GGGAATTTAA AGGTGATGAT ATTACAATTT ATGGCAGTGA TTTTATGGAC 
TTGACAACTA ACCACACTAT ACAGAATTTA CCCTCAGCAG ATAATTCATC TAATAAAGAA 
AATCAAACTC AGAATGTCAT GATGGAGGTT AAAGCTCCAG GAGGGAAGAC AATCTTTAAT 
AAACTGAATA TTCCTTTTCA AACCCCTTTA AACCCTAATG GTGAAATACA TATTACCAAA 
AGTCATATTA CAGGGACAGA AACTCACACA AGCACACAGA CTTCTAACCA AGATGCCAGA 
ACATTGACTA TAATCCCAGA ATCTATATGT TCCAGTCCAG CTACTCAAGA TTATAAGACA 
TTTTTCTATT CTAGCAGTAA TAATGCCATG GAACTGACCA CGTGTCTCTC GGGTATGAGA 
AAAGAGAAGA ATTTGCTGAA GAATGATAAT TATTCTAAAA TGTATCCCAA TCCAGATGCC 
ATCTCTCTTC TTACAGAGAA AACTATTTAT TCAGGAGAGG ATAGCATGGA CATTACGAGG 
AGTCATACAG TTGCAATAGA TAATCAGATT TTTAAACAAG ATCAGACAAA TATACAGAAA 
GCAGACACAT CAATATCTGA AAAAGAAATA ATGCTCCAAA ATCTCATAAC CATGTCAAAG 
GACGGGAAAA TGAATATAAA TTGTAGCTCA GTTCCTCATA TATCTAAGGG AAGATTACAG 
CAGAGCCTGG CAAACTCTTT ATCTATTTCA TTGACTGACA AAAAGACTGA AATCTTCACA 
GGTGAAGATA TGGATTTGAC TAAAAGTCAC ACAACTAACT TAGGAAGTCA AGCTCCACTT 
ACATCTTACA ATGTAACATC TGAGAATACC AGTAAATTTC TCTCTCACAG CAAAAGCCCT 
TCAGGTGAAT GGCAAGAAAT GACCAAAAGT CGTATTGAAC CATCCCAGCA GCCAGACATA 
ATATCTAAAA ACATCCCAGT GGACACCTGG GGCAAAGAAA AAGTTCAAGT TTTAGAGATT 
GCACCTTATG TTGATAAAGA TTCTCCTCAA TCAGCTGATA TTAATCAAGA CATTGCAATA 
AGCCATAGTA TAGTATACTC TGATAGAAAT CTTGAGAAAC AAGTAGCACT AGGAAATAAT 
AGAAATACTG TTCCATGTGA GCAATCTTTG TTTCCTACTT CAGAGTCATT ATTTTCATTA 
GAAGGACAGT CTAACATGAA AAATCATAAT ATTGCTATTA ACAGTTACAC AATGAAATCT 
GTGCCAGGCC AGAATTCTAA ACTGCCTGAG CCACTGAGGA AAGGTGTAGG CAATCCAACA 
CCTGACTATT CTCATGACAA AACAATTATT TGTTCAGAGG AGGAACAAAA TATGGATCTA 
ACCAAGAGTC ACACTGTTGT CATTGGATTT AGTCCTTCTG AAATACAAGA ATTGGGTAAG 
ACTAATCTAG AAAATACTAA CAGTCTGCTA ACAACAGCAA ACAGACAGAC AGCTATAAAA 
GTTGAAAAAT GTTGTAAAAG TCCTATAGAA AAAACAGGAA TATCTGTTTC TAAGGATAAC 
CTAGATGTGA TAGAGGACAA TAGCATACAG AAACTTGGAT TTTTGAATGA AAAGCAAGAT 
GTCAAAATTT GTGGAAGAAA AAGTGTTGGC AGACAAAAAG TTGATAAGAC TATTGTATTT 
TCAGAGGGTG ATGAGAATGA TATGGATATC ACCAAGATTT TTACAGTGGA AATAAATCAT 
AAACCTTTAT TACTTGAACA AGATTCCCAT TTGGCAGGAA CTTCTAAAAC TGTTTTGTAT 
GAATGTGGGC AAGATGATGT GGAGATCACT AAAAGTCACA CAACTGCCTT GGAATGTATA 
ACTGTCTCAC CAGATAAAAT AACCAGTAGG CCTATGGACA AAACTGTAAT GTTTGTAGAT 
AATTACAATG AACTGGAAAT GACAAAGTCT CACACTGTTT TCATTGACTA CCAAGCAAAG 
GAGAGGACTG TGAGTATAGA CAAACTTGAC ACGGAACTAT CCAAAAGAAG AAAAAGCATA 
GAAAAGCCAA AAATGACACC TGCTTCTGCT GAGGAGAGTG TTTCCTTTCT GGAGAATGGT 
GAAAGTGACC ATTCAGCAGC AAACGTCAAT AAGCTAACAC TACTGAGGGA ATGGTCTAAT 
AATGGTCCTA TAGAGAAAGC AGTAGCTGGT GATAACATGG AAGTATCTAA ATTCACCATT 
TGGAAAAATG GCAAAGATGT ACAAAAGCCT GAATTTCTGA ATGACACTAT ATCAGACAAA 
AGTCAGAGAA GGAAAAGCCT TAGACTCAAA AATGACAAGA TTGCTGCATT TTCTAAAAAT 
GATGATGATA TGGATATCAC CCAGAGTTGT ACAGTGAAAA TAAACTATAA AAGTGCCCCA 
GAAGATAGAG AGGATTCCCA TTTGGTGCCT TTGACAGGAG CTTCTAAAAC TGTTTTGTAT 
TCATGTGGGC AGGATGACAT GGAGATGACT AGAAGTCATA CGACTGCGTT AGAATGTAAA 
ACTGTCTCAC CGGATAAAAT CACTACTAGG CCCATAGATA AAACTGTAAT GTTTGTTGAT 
AATCATGGTG ATCTGGAAGT CACCGAGTCC CATACTGTTT TCATTGATTG TCAAAACACA 
GAGAAAATTA ATCAAGAGTG CCCTAAATTT GGAATAGCTA CAGGAAAACC CTTGGGTATT 
TCTTTTCATA ATGATGGTAG CTCTGTTCAA GAAATCACTG AAAAACAAGC ATTGGCAGTA 
GACCAGACCT ATATACCGGA AAAAGCCAGA ATTGGAAGCT GTCAGTTAAA TAGTACAGAT 
AGAAGAAACA TGGACTTTAC TAACAGTCAA GCAACTGCTA CGTGTGGATC CAGTGATAAT 
TATTCCTGTT TACCAAATGC TATTTCCTGT TTTGATAATT TAGAGGGGAA TGTCATGTCC 
TTAAGTAATA AAGATAAGAA AGCCAGTAAC TGCCCAGTGC AAAATGATCT TTCTTATGTA 
AAGGATTTAG CCAATGAATA TTACTTGAAA CCTGAGGGAC TGCCTCCCTC TGCTTGTTCT 
TTGTTAAAGA AAGAAAAAGT GACTCAAACC AATACCAATG GACAGTTAGA CTGTGTTACA 
ACAGTGCTTA AAGATCAAGA TATGATTAAG GAGTCCCAGA ATCTAATAGC TAATCAAACT 
TTACAAGATA GTCAGGAGCT GGCGGAGATG ATTAAACCTA ATTCAAATCG AGTATCCTTT 
AAACCCCCAA AGGATCAAAT GGAGGGCTTT GTTGATACCA CGGAACATAA TTTAAAGAAG 
ACTGTTGTTG ATAATGTTTG TGTTACTTCT CAGCCTCATC TCACAAGCCA GGTACCTCCA 
TTACCTCAAC AAGGACGGAT AATTTTGAAT AAAGATGAAA CAATATCATC TAATTCTGGA 
AATAAGAATT TAAATATTGT TATAGGAAAT TCCTTTGTAC CCACATGTGA AAACGAGGCC 
AAAATGCTCA ACAATGATAA ACAATTTACC ATAGTCTGTA AAAATGAGCT GAGGAAAAAT 
ACTAAATGTA ATACCGATTT CCACAGTAAT TCAGACTTGA CTAAGCAAGT TATTCAAACT 
CATACTAATT CTAGAGAAGC ATCAGATACT GTAATTGCAT CTAATACAAG TTTTAATAGT 
GTCAGATCAA ATCTGAATAA TTTGAATGGA AAAACTGAAG AATTTTTAGA TTTCCAAACT 
CCCCATATTC CATCTTCTCC AGAGCAAATA CTTGCATTAA TAAACAAGGC ACACAATCAT 
ATGAGTATAT TGCAAGCTAC AGAAATACAG AACATTAACC CAGCGCCCAG CAATGTTGAA 
GATGATAAAG ATGAAGAAAG CAAAATTTCT CATAATGGAG CTGAAACCAA CTCTGTACCA 
CTCATGACAG TTGTAAAAGA TAAAGTGAAG AGATGTTCTT TGGGAATATT TTTGCCCAGA 
TTGCCAAACA AGAGAAATTG TAGTGTCACT GGTATTAATG ACCTGAAGCA GATTCAAGGA 
GACACAACTG ATTTAAATCA CTTAGAAATT CAGCCAGTCT TCAGTAAGGA GCCAAGCATT 
GAATTTGTTG CAACTAAACT GACCTTAAGT CCATCTCAGT ATATAAATGA GGAAAACTTT 
CCTATATATC CTGGTGAGAT TAATTCCTCA GATTCTATTA CCATAGAAAC TGAGGAAAAG 
GCTTTGATTG AGACATATCA AAAAGGGATT CCACCATCTG AAAATAAGAT AAGAGAAACC 
TGCAGTAACC AAAAAAGAAC CTGGGTACAA GAAGATGATG ATATTCAGAA TGAGAAAAAA 
ATCAGGAAAA GTGAGATTAG GTGTAGTGAT ACTACACAAG ATCAGGAGAT TTTTGATCAC 
CATGCTGAAG GGGATATAGA TAAAAATGCT AACAGCCTGA GCAGAACCCC ATCTAGTTGC 
AGCAGTTCTC TGGATTCGAT AAAGGCTGAT GGGACCTCTC TGGACTTCAG CACACAGCAC 
AATAGTCAAA TGGAATCACA GTTTCTCAGA GACACTATTT GTGAAGAGAG CTTGAAGGAG 
AAACTCAAAG ATGGGAAAAT TACAATAAAG GAGTTCTTTA TACTTCTTCA GGTCCACATT 
TTGATACAGA AACCCCGACA GAGCAATCTC CCAGCCAAAT TTACTATAAA CACACCACCT 
ACTCTGGAAG ACCTGATGTT AAGTCAGTAT GTTTACCGAC CCAAGATACA GATTTATAGA 
GAAGATTGTG AAGCTCTTTG CCAAAAGATT GAAGAATTAA AGCTTTCTGC ATTGAACCAG 
GATAAATTGT TGACTGACAT AAATAGGAAC CTGTGGGAAA AAATGAGACA CTGCTCTGAT 
GAAGAGCTGA AGGTCTTTGG AATTTACCTG AACAAAATAA AATCACATTT TACCAAGATG 
ACTAAAGTTT TTACTCACCA AGGAAAAGTG GCTCTCTATA CCAAGCTGGT ACAGTCAGCT 
CAGAATGAGA GGAAGAAACT TCAGATAAGG ATAGATGAGA TGGACAACAT ACTTAAGAAG 
ATTGAGAACT GTCTCGCTGA AGTTGAAATA GAAACTAAGA ATTTGGATGA AGAGAAAGAC 
AATCCTATGG AAGAATGGGA TTCTGAAATG AGAGCCGCAG AGAAAGAACT AGAACAGCTG 
AAAACTGAAG AAGATGAGCT CCAAAGAAAT CTCTTAGAAC TGGAAGTACA GAAAGAGCAG 
ACCCTTGCTC AAATAGACTT TGTACAAAAA CAAACAGATA GAACTGAAGA GCTAATGGAT 
CAGTTGAGCT TGTCTGAGTG GGAAGCCATT GAGTGGAGTG ATGATCAAGC TGTATTCACC 
TTTCTTTATG ACACAATAGA ACTTACTATC ACCTTTGGAG AGACAGTAGC TGGAAGTCCC 
AAGATCAGGC TGCCAGCATG GTCAGTTGAT CTCCCTTTCC TGGACAAGGC TTGTAGGAAG 
ATTGTTGACT TGAATTTTCA ATCTCGGTTA GATGAGGATA AAGCTCCTCC TTCCTCCCTT 
TTAGTTCATA AGCTTATTTT CCAGTACATT GAGGAAAAGG AATCCTGGAA GAAAAAATGT 
ACAACACAGC ATCAAATACC CAAGATGCTT CAAGAAATCT CATTGGTAGT GAGCCATTGT 
AGACTCCTTG GAGAGGAAAT TGAGTTTTTA AAGAGATGGG GACCAAATTA TAACCTAATG 
AACATAGATG TGACTAATAC TGAATTGAGG CTTTTATTCT CCAGCTCTGC AGCATTTGCA 
AAATTTGAGA TAACTTTGCC TCTCTCAGTC CATTTTCCAA CTGTTCCATT ACCTTTCTCC 
ATTCAAAAGC ACCTTGGAAA CATTGGCCAA GATGAAATCA CTGCAATTCT ATCTAAAGTG 
CCACTGAAGA ACAACTATCT AAAGAATGTA GTCAAGCAAA TTTACCAAGA TCTGCTTCAG 
GACTGCCATT TCTATCACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KNL1 ( XM_014109668.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013965143.1 Canis lupus familiaris kinetochore scaffold 1 (KNL1), transcript variant X2, mRNA.

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CloneID	OCe28661
Clone ID Related Accession (Same CDS sequence)	XM_014109669.1 , XM_005638255.2 , XM_005638255.1 , XM_014109668.1 , XM_014109668.2
Accession Version	XM_014109668.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6861bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	kinetochore scaffold 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006612.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014109668.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGGGA TGTCTTCAGA GACTAATGAA GAGAATGACA ATATGGAGAG ACCTGTTAGA 
AGACGACATT CCTCCATTTT GAAACCCCCA AGGAGTCCTC TTCAGGACCT CAGAGGTGGG 
AATGAAGCAG TTCAGGAATC TAATGCTTTG AGAAATAAGA AAAGCTCTCG TCGAGTCAGC 
TTTGCAGATA CCATAAAAGT ATTCCAAACA GAGTCTCATA TGAAAATAGT AAGAAAGTCA 
GAAATTGCAG AAACAGAAGC AGGAGAAAAT GTTCTATATA TACAGAATAA GAAGCCAGAA 
GAAACCTACT GTGAAATTAC TGGGATGAAC ACATTGCTTT CTGCTCCTAT TCAGACTCAG 
ATGCAACAGA GAGAGATTTC AATTACAGAA CACAACCATG AAATGAAACC TGCAAATGAC 
CAAACAGTCA TCTTTTCAGA TGAAAACCAG ATGGACCTAA CAGCAAGTCA CACCGTAATG 
ATTACCAAAG ACCTGTTAGA TTGTACTAAA AGTGAAAAGT CCATTAAGAT AGATACCGCA 
TCATTTCTGG CTAACTTAAA GCTCCATACT AAGGATTCAA GAATGAAAAA AGAATTAAAT 
TTTTCCATGG ATCAAAGCAC TTCTTCAGAA AAGAAAATAC ATTTTAATGA CTTCATAAAA 
AGATTGAAAA CAGGAAAATC TAATGCTTTT CCTACAGGAC CTGATAAAGA AAATTTTGAA 
ATATCTGTTC ATCCCATAAA AACAAAGAAG GCCTCTTCTG TACATCAAAT GCATGTATCT 
CTTAGGGTAG ATGAAAATAG TAGTAATACA ACTAGAATCT TCGGAGAACA AAATGATGGG 
ATGAATTTCA CTCAGTGTCA TACAGCCAGT ATTCAGACTT TGGTTCCCAC ATCCAGTAAG 
GCCAGCATAG GGGAATTTAA AGGTGATGAT ATTACAATTT ATGGCAGTGA TTTTATGGAC 
TTGACAACTA ACCACACTAT ACAGAATTTA CCCTCAGCAG ATAATTCATC TAATAAAGAA 
AATCAAACTC AGAATGTCAT GATGGAGGTT AAAGCTCCAG GAGGGAAGAC AATCTTTAAT 
AAACTGAATA TTCCTTTTCA AACCCCTTTA AACCCTAATG GTGAAATACA TATTACCAAA 
AGTCATATTA CAGGGACAGA AACTCACACA AGCACACAGA CTTCTAACCA AGATGCCAGA 
ACATTGACTA TAATCCCAGA ATCTATATGT TCCAGTCCAG CTACTCAAGA TTATAAGACA 
TTTTTCTATT CTAGCAGTAA TAATGCCATG GAACTGACCA CGTGTCTCTC GGGTATGAGA 
AAAGAGAAGA ATTTGCTGAA GAATGATAAT TATTCTAAAA TGTATCCCAA TCCAGATGCC 
ATCTCTCTTC TTACAGAGAA AACTATTTAT TCAGGAGAGG ATAGCATGGA CATTACGAGG 
AGTCATACAG TTGCAATAGA TAATCAGATT TTTAAACAAG ATCAGACAAA TATACAGAAA 
GCAGACACAT CAATATCTGA AAAAGAAATA ATGCTCCAAA ATCTCATAAC CATGTCAAAG 
GACGGGAAAA TGAATATAAA TTGTAGCTCA GTTCCTCATA TATCTAAGGG AAGATTACAG 
CAGAGCCTGG CAAACTCTTT ATCTATTTCA TTGACTGACA AAAAGACTGA AATCTTCACA 
GGTGAAGATA TGGATTTGAC TAAAAGTCAC ACAACTAACT TAGGAAGTCA AGCTCCACTT 
ACATCTTACA ATGTAACATC TGAGAATACC AGTAAATTTC TCTCTCACAG CAAAAGCCCT 
TCAGGTGAAT GGCAAGAAAT GACCAAAAGT CGTATTGAAC CATCCCAGCA GCCAGACATA 
ATATCTAAAA ACATCCCAGT GGACACCTGG GGCAAAGAAA AAGTTCAAGT TTTAGAGATT 
GCACCTTATG TTGATAAAGA TTCTCCTCAA TCAGCTGATA TTAATCAAGA CATTGCAATA 
AGCCATAGTA TAGTATACTC TGATAGAAAT CTTGAGAAAC AAGTAGCACT AGGAAATAAT 
AGAAATACTG TTCCATGTGA GCAATCTTTG TTTCCTACTT CAGAGTCATT ATTTTCATTA 
GAAGGACAGT CTAACATGAA AAATCATAAT ATTGCTATTA ACAGTTACAC AATGAAATCT 
GTGCCAGGCC AGAATTCTAA ACTGCCTGAG CCACTGAGGA AAGGTGTAGG CAATCCAACA 
CCTGACTATT CTCATGACAA AACAATTATT TGTTCAGAGG AGGAACAAAA TATGGATCTA 
ACCAAGAGTC ACACTGTTGT CATTGGATTT AGTCCTTCTG AAATACAAGA ATTGGGTAAG 
ACTAATCTAG AAAATACTAA CAGTCTGCTA ACAACAGCAA ACAGACAGAC AGCTATAAAA 
GTTGAAAAAT GTTGTAAAAG TCCTATAGAA AAAACAGGAA TATCTGTTTC TAAGGATAAC 
CTAGATGTGA TAGAGGACAA TAGCATACAG AAACTTGGAT TTTTGAATGA AAAGCAAGAT 
GTCAAAATTT GTGGAAGAAA AAGTGTTGGC AGACAAAAAG TTGATAAGAC TATTGTATTT 
TCAGAGGGTG ATGAGAATGA TATGGATATC ACCAAGATTT TTACAGTGGA AATAAATCAT 
AAACCTTTAT TACTTGAACA AGATTCCCAT TTGGCAGGAA CTTCTAAAAC TGTTTTGTAT 
GAATGTGGGC AAGATGATGT GGAGATCACT AAAAGTCACA CAACTGCCTT GGAATGTATA 
ACTGTCTCAC CAGATAAAAT AACCAGTAGG CCTATGGACA AAACTGTAAT GTTTGTAGAT 
AATTACAATG AACTGGAAAT GACAAAGTCT CACACTGTTT TCATTGACTA CCAAGCAAAG 
GAGAGGACTG TGAGTATAGA CAAACTTGAC ACGGAACTAT CCAAAAGAAG AAAAAGCATA 
GAAAAGCCAA AAATGACACC TGCTTCTGCT GAGGAGAGTG TTTCCTTTCT GGAGAATGGT 
GAAAGTGACC ATTCAGCAGC AAACGTCAAT AAGCTAACAC TACTGAGGGA ATGGTCTAAT 
AATGGTCCTA TAGAGAAAGC AGTAGCTGGT GATAACATGG AAGTATCTAA ATTCACCATT 
TGGAAAAATG GCAAAGATGT ACAAAAGCCT GAATTTCTGA ATGACACTAT ATCAGACAAA 
AGTCAGAGAA GGAAAAGCCT TAGACTCAAA AATGACAAGA TTGCTGCATT TTCTAAAAAT 
GATGATGATA TGGATATCAC CCAGAGTTGT ACAGTGAAAA TAAACTATAA AAGTGCCCCA 
GAAGATAGAG AGGATTCCCA TTTGGTGCCT TTGACAGGAG CTTCTAAAAC TGTTTTGTAT 
TCATGTGGGC AGGATGACAT GGAGATGACT AGAAGTCATA CGACTGCGTT AGAATGTAAA 
ACTGTCTCAC CGGATAAAAT CACTACTAGG CCCATAGATA AAACTGTAAT GTTTGTTGAT 
AATCATGGTG ATCTGGAAGT CACCGAGTCC CATACTGTTT TCATTGATTG TCAAAACACA 
GAGAAAATTA ATCAAGAGTG CCCTAAATTT GGAATAGCTA CAGGAAAACC CTTGGGTATT 
TCTTTTCATA ATGATGGTAG CTCTGTTCAA GAAATCACTG AAAAACAAGC ATTGGCAGTA 
GACCAGACCT ATATACCGGA AAAAGCCAGA ATTGGAAGCT GTCAGTTAAA TAGTACAGAT 
AGAAGAAACA TGGACTTTAC TAACAGTCAA GCAACTGCTA CGTGTGGATC CAGTGATAAT 
TATTCCTGTT TACCAAATGC TATTTCCTGT TTTGATAATT TAGAGGGGAA TGTCATGTCC 
TTAAGTAATA AAGATAAGAA AGCCAGTAAC TGCCCAGTGC AAAATGATCT TTCTTATGTA 
AAGGATTTAG CCAATGAATA TTACTTGAAA CCTGAGGGAC TGCCTCCCTC TGCTTGTTCT 
TTGTTAAAGA AAGAAAAAGT GACTCAAACC AATACCAATG GACAGTTAGA CTGTGTTACA 
ACAGTGCTTA AAGATCAAGA TATGATTAAG GAGTCCCAGA ATCTAATAGC TAATCAAACT 
TTACAAGATA GTCAGGAGCT GGCGGAGATG ATTAAACCTA ATTCAAATCG AGTATCCTTT 
AAACCCCCAA AGGATCAAAT GGAGGGCTTT GTTGATACCA CGGAACATAA TTTAAAGAAG 
ACTGTTGTTG ATAATGTTTG TGTTACTTCT CAGCCTCATC TCACAAGCCA GGTACCTCCA 
TTACCTCAAC AAGGACGGAT AATTTTGAAT AAAGATGAAA CAATATCATC TAATTCTGGA 
AATAAGAATT TAAATATTGT TATAGGAAAT TCCTTTGTAC CCACATGTGA AAACGAGGCC 
AAAATGCTCA ACAATGATAA ACAATTTACC ATAGTCTGTA AAAATGAGCT GAGGAAAAAT 
ACTAAATGTA ATACCGATTT CCACAGTAAT TCAGACTTGA CTAAGCAAGT TATTCAAACT 
CATACTAATT CTAGAGAAGC ATCAGATACT GTAATTGCAT CTAATACAAG TTTTAATAGT 
GTCAGATCAA ATCTGAATAA TTTGAATGGA AAAACTGAAG AATTTTTAGA TTTCCAAACT 
CCCCATATTC CATCTTCTCC AGAGCAAATA CTTGCATTAA TAAACAAGGC ACACAATCAT 
ATGAGTATAT TGCAAGCTAC AGAAATACAG AACATTAACC CAGCGCCCAG CAATGTTGAA 
GATGATAAAG ATGAAGAAAG CAAAATTTCT CATAATGGAG CTGAAACCAA CTCTGTACCA 
CTCATGACAG TTGTAAAAGA TAAAGTGAAG AGATGTTCTT TGGGAATATT TTTGCCCAGA 
TTGCCAAACA AGAGAAATTG TAGTGTCACT GGTATTAATG ACCTGAAGCA GATTCAAGGA 
GACACAACTG ATTTAAATCA CTTAGAAATT CAGCCAGTCT TCAGTAAGGA GCCAAGCATT 
GAATTTGTTG CAACTAAACT GACCTTAAGT CCATCTCAGT ATATAAATGA GGAAAACTTT 
CCTATATATC CTGGTGAGAT TAATTCCTCA GATTCTATTA CCATAGAAAC TGAGGAAAAG 
GCTTTGATTG AGACATATCA AAAAGGGATT CCACCATCTG AAAATAAGAT AAGAGAAACC 
TGCAGTAACC AAAAAAGAAC CTGGGTACAA GAAGATGATG ATATTCAGAA TGAGAAAAAA 
ATCAGGAAAA GTGAGATTAG GTGTAGTGAT ACTACACAAG ATCAGGAGAT TTTTGATCAC 
CATGCTGAAG GGGATATAGA TAAAAATGCT AACAGCCTGA GCAGAACCCC ATCTAGTTGC 
AGCAGTTCTC TGGATTCGAT AAAGGCTGAT GGGACCTCTC TGGACTTCAG CACACAGCAC 
AATAGTCAAA TGGAATCACA GTTTCTCAGA GACACTATTT GTGAAGAGAG CTTGAAGGAG 
AAACTCAAAG ATGGGAAAAT TACAATAAAG GAGTTCTTTA TACTTCTTCA GGTCCACATT 
TTGATACAGA AACCCCGACA GAGCAATCTC CCAGCCAAAT TTACTATAAA CACACCACCT 
ACTCTGGAAG ACCTGATGTT AAGTCAGTAT GTTTACCGAC CCAAGATACA GATTTATAGA 
GAAGATTGTG AAGCTCTTTG CCAAAAGATT GAAGAATTAA AGCTTTCTGC ATTGAACCAG 
GATAAATTGT TGACTGACAT AAATAGGAAC CTGTGGGAAA AAATGAGACA CTGCTCTGAT 
GAAGAGCTGA AGGTCTTTGG AATTTACCTG AACAAAATAA AATCACATTT TACCAAGATG 
ACTAAAGTTT TTACTCACCA AGGAAAAGTG GCTCTCTATA CCAAGCTGGT ACAGTCAGCT 
CAGAATGAGA GGAAGAAACT TCAGATAAGG ATAGATGAGA TGGACAACAT ACTTAAGAAG 
ATTGAGAACT GTCTCGCTGA AGTTGAAATA GAAACTAAGA ATTTGGATGA AGAGAAAGAC 
AATCCTATGG AAGAATGGGA TTCTGAAATG AGAGCCGCAG AGAAAGAACT AGAACAGCTG 
AAAACTGAAG AAGATGAGCT CCAAAGAAAT CTCTTAGAAC TGGAAGTACA GAAAGAGCAG 
ACCCTTGCTC AAATAGACTT TGTACAAAAA CAAACAGATA GAACTGAAGA GCTAATGGAT 
CAGTTGAGCT TGTCTGAGTG GGAAGCCATT GAGTGGAGTG ATGATCAAGC TGTATTCACC 
TTTCTTTATG ACACAATAGA ACTTACTATC ACCTTTGGAG AGACAGTAGC TGGAAGTCCC 
AAGATCAGGC TGCCAGCATG GTCAGTTGAT CTCCCTTTCC TGGACAAGGC TTGTAGGAAG 
ATTGTTGACT TGAATTTTCA ATCTCGGTTA GATGAGGATA AAGCTCCTCC TTCCTCCCTT 
TTAGTTCATA AGCTTATTTT CCAGTACATT GAGGAAAAGG AATCCTGGAA GAAAAAATGT 
ACAACACAGC ATCAAATACC CAAGATGCTT CAAGAAATCT CATTGGTAGT GAGCCATTGT 
AGACTCCTTG GAGAGGAAAT TGAGTTTTTA AAGAGATGGG GACCAAATTA TAACCTAATG 
AACATAGATG TGACTAATAC TGAATTGAGG CTTTTATTCT CCAGCTCTGC AGCATTTGCA 
AAATTTGAGA TAACTTTGCC TCTCTCAGTC CATTTTCCAA CTGTTCCATT ACCTTTCTCC 
ATTCAAAAGC ACCTTGGAAA CATTGGCCAA GATGAAATCA CTGCAATTCT ATCTAAAGTG 
CCACTGAAGA ACAACTATCT AAAGAATGTA GTCAAGCAAA TTTACCAAGA TCTGCTTCAG 
GACTGCCATT TCTATCACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013965143.1
CDS	301..7161
Translation	MDGMSSETNEENDNMERPVRRRHSSILKPPRSPLQDLRGGNEAVQESNALRNKKSSRRVSFADTIKVFQTESHMKIVRKSEIAETEAGENVLYIQNKKPEETYCEITGMNTLLSAPIQTQMQQREISITEHNHEMKPANDQTVIFSDENQMDLTASHTVMITKDLLDCTKSEKSIKIDTASFLANLKLHTKDSRMKKELNFSMDQSTSSEKKIHFNDFIKRLKTGKSNAFPTGPDKENFEISVHPIKTKKASSVHQMHVSLRVDENSSNTTRIFGEQNDGMNFTQCHTASIQTLVPTSSKASIGEFKGDDITIYGSDFMDLTTNHTIQNLPSADNSSNKENQTQNVMMEVKAPGGKTIFNKLNIPFQTPLNPNGEIHITKSHITGTETHTSTQTSNQDARTLTIIPESICSSPATQDYKTFFYSSSNNAMELTTCLSGMRKEKNLLKNDNYSKMYPNPDAISLLTEKTIYSGEDSMDITRSHTVAIDNQIFKQDQTNIQKADTSISEKEIMLQNLITMSKDGKMNINCSSVPHISKGRLQQSLANSLSISLTDKKTEIFTGEDMDLTKSHTTNLGSQAPLTSYNVTSENTSKFLSHSKSPSGEWQEMTKSRIEPSQQPDIISKNIPVDTWGKEKVQVLEIAPYVDKDSPQSADINQDIAISHSIVYSDRNLEKQVALGNNRNTVPCEQSLFPTSESLFSLEGQSNMKNHNIAINSYTMKSVPGQNSKLPEPLRKGVGNPTPDYSHDKTIICSEEEQNMDLTKSHTVVIGFSPSEIQELGKTNLENTNSLLTTANRQTAIKVEKCCKSPIEKTGISVSKDNLDVIEDNSIQKLGFLNEKQDVKICGRKSVGRQKVDKTIVFSEGDENDMDITKIFTVEINHKPLLLEQDSHLAGTSKTVLYECGQDDVEITKSHTTALECITVSPDKITSRPMDKTVMFVDNYNELEMTKSHTVFIDYQAKERTVSIDKLDTELSKRRKSIEKPKMTPASAEESVSFLENGESDHSAANVNKLTLLREWSNNGPIEKAVAGDNMEVSKFTIWKNGKDVQKPEFLNDTISDKSQRRKSLRLKNDKIAAFSKNDDDMDITQSCTVKINYKSAPEDREDSHLVPLTGASKTVLYSCGQDDMEMTRSHTTALECKTVSPDKITTRPIDKTVMFVDNHGDLEVTESHTVFIDCQNTEKINQECPKFGIATGKPLGISFHNDGSSVQEITEKQALAVDQTYIPEKARIGSCQLNSTDRRNMDFTNSQATATCGSSDNYSCLPNAISCFDNLEGNVMSLSNKDKKASNCPVQNDLSYVKDLANEYYLKPEGLPPSACSLLKKEKVTQTNTNGQLDCVTTVLKDQDMIKESQNLIANQTLQDSQELAEMIKPNSNRVSFKPPKDQMEGFVDTTEHNLKKTVVDNVCVTSQPHLTSQVPPLPQQGRIILNKDETISSNSGNKNLNIVIGNSFVPTCENEAKMLNNDKQFTIVCKNELRKNTKCNTDFHSNSDLTKQVIQTHTNSREASDTVIASNTSFNSVRSNLNNLNGKTEEFLDFQTPHIPSSPEQILALINKAHNHMSILQATEIQNINPAPSNVEDDKDEESKISHNGAETNSVPLMTVVKDKVKRCSLGIFLPRLPNKRNCSVTGINDLKQIQGDTTDLNHLEIQPVFSKEPSIEFVATKLTLSPSQYINEENFPIYPGEINSSDSITIETEEKALIETYQKGIPPSENKIRETCSNQKRTWVQEDDDIQNEKKIRKSEIRCSDTTQDQEIFDHHAEGDIDKNANSLSRTPSSCSSSLDSIKADGTSLDFSTQHNSQMESQFLRDTICEESLKEKLKDGKITIKEFFILLQVHILIQKPRQSNLPAKFTINTPPTLEDLMLSQYVYRPKIQIYREDCEALCQKIEELKLSALNQDKLLTDINRNLWEKMRHCSDEELKVFGIYLNKIKSHFTKMTKVFTHQGKVALYTKLVQSAQNERKKLQIRIDEMDNILKKIENCLAEVEIETKNLDEEKDNPMEEWDSEMRAAEKELEQLKTEEDELQRNLLELEVQKEQTLAQIDFVQKQTDRTEELMDQLSLSEWEAIEWSDDQAVFTFLYDTIELTITFGETVAGSPKIRLPAWSVDLPFLDKACRKIVDLNFQSRLDEDKAPPSSLLVHKLIFQYIEEKESWKKKCTTQHQIPKMLQEISLVVSHCRLLGEEIEFLKRWGPNYNLMNIDVTNTELRLLFSSSAAFAKFEITLPLSVHFPTVPLPFSIQKHLGNIGQDEITAILSKVPLKNNYLKNVVKQIYQDLLQDCHFYH

Target ORF information:

RefSeq Version	XM_014109668.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris kinetochore scaffold 1 (KNL1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014109668.2

ORF Insert Sequence:

ATGGATGGGA TGTCTTCAGA GACTAATGAA GAGAATGACA ATATGGAGAG ACCTGTTAGA 
AGACGACATT CCTCCATTTT GAAACCCCCA AGGAGTCCTC TTCAGGACCT CAGAGGTGGG 
AATGAAGCAG TTCAGGAATC TAATGCTTTG AGAAATAAGA AAAGCTCTCG TCGAGTCAGC 
TTTGCAGATA CCATAAAAGT ATTCCAAACA GAGTCTCATA TGAAAATAGT AAGAAAGTCA 
GAAATTGCAG AAACAGAAGC AGGAGAAAAT GTTCTATATA TACAGAATAA GAAGCCAGAA 
GAAACCTACT GTGAAATTAC TGGGATGAAC ACATTGCTTT CTGCTCCTAT TCAGACTCAG 
ATGCAACAGA GAGAGATTTC AATTACAGAA CACAACCATG AAATGAAACC TGCAAATGAC 
CAAACAGTCA TCTTTTCAGA TGAAAACCAG ATGGACCTAA CAGCAAGTCA CACCGTAATG 
ATTACCAAAG ACCTGTTAGA TTGTACTAAA AGTGAAAAGT CCATTAAGAT AGATACCGCA 
TCATTTCTGG CTAACTTAAA GCTCCATACT AAGGATTCAA GAATGAAAAA AGAATTAAAT 
TTTTCCATGG ATCAAAGCAC TTCTTCAGAA AAGAAAATAC ATTTTAATGA CTTCATAAAA 
AGATTGAAAA CAGGAAAATC TAATGCTTTT CCTACAGGAC CTGATAAAGA AAATTTTGAA 
ATATCTGTTC ATCCCATAAA AACAAAGAAG GCCTCTTCTG TACATCAAAT GCATGTATCT 
CTTAGGGTAG ATGAAAATAG TAGTAATACA ACTAGAATCT TCGGAGAACA AAATGATGGG 
ATGAATTTCA CTCAGTGTCA TACAGCCAGT ATTCAGACTT TGGTTCCCAC ATCCAGTAAG 
GCCAGCATAG GGGAATTTAA AGGTGATGAT ATTACAATTT ATGGCAGTGA TTTTATGGAC 
TTGACAACTA ACCACACTAT ACAGAATTTA CCCTCAGCAG ATAATTCATC TAATAAAGAA 
AATCAAACTC AGAATGTCAT GATGGAGGTT AAAGCTCCAG GAGGGAAGAC AATCTTTAAT 
AAACTGAATA TTCCTTTTCA AACCCCTTTA AACCCTAATG GTGAAATACA TATTACCAAA 
AGTCATATTA CAGGGACAGA AACTCACACA AGCACACAGA CTTCTAACCA AGATGCCAGA 
ACATTGACTA TAATCCCAGA ATCTATATGT TCCAGTCCAG CTACTCAAGA TTATAAGACA 
TTTTTCTATT CTAGCAGTAA TAATGCCATG GAACTGACCA CGTGTCTCTC GGGTATGAGA 
AAAGAGAAGA ATTTGCTGAA GAATGATAAT TATTCTAAAA TGTATCCCAA TCCAGATGCC 
ATCTCTCTTC TTACAGAGAA AACTATTTAT TCAGGAGAGG ATAGCATGGA CATTACGAGG 
AGTCATACAG TTGCAATAGA TAATCAGATT TTTAAACAAG ATCAGACAAA TATACAGAAA 
GCAGACACAT CAATATCTGA AAAAGAAATA ATGCTCCAAA ATCTCATAAC CATGTCAAAG 
GACGGGAAAA TGAATATAAA TTGTAGCTCA GTTCCTCATA TATCTAAGGG AAGATTACAG 
CAGAGCCTGG CAAACTCTTT ATCTATTTCA TTGACTGACA AAAAGACTGA AATCTTCACA 
GGTGAAGATA TGGATTTGAC TAAAAGTCAC ACAACTAACT TAGGAAGTCA AGCTCCACTT 
ACATCTTACA ATGTAACATC TGAGAATACC AGTAAATTTC TCTCTCACAG CAAAAGCCCT 
TCAGGTGAAT GGCAAGAAAT GACCAAAAGT CGTATTGAAC CATCCCAGCA GCCAGACATA 
ATATCTAAAA ACATCCCAGT GGACACCTGG GGCAAAGAAA AAGTTCAAGT TTTAGAGATT 
GCACCTTATG TTGATAAAGA TTCTCCTCAA TCAGCTGATA TTAATCAAGA CATTGCAATA 
AGCCATAGTA TAGTATACTC TGATAGAAAT CTTGAGAAAC AAGTAGCACT AGGAAATAAT 
AGAAATACTG TTCCATGTGA GCAATCTTTG TTTCCTACTT CAGAGTCATT ATTTTCATTA 
GAAGGACAGT CTAACATGAA AAATCATAAT ATTGCTATTA ACAGTTACAC AATGAAATCT 
GTGCCAGGCC AGAATTCTAA ACTGCCTGAG CCACTGAGGA AAGGTGTAGG CAATCCAACA 
CCTGACTATT CTCATGACAA AACAATTATT TGTTCAGAGG AGGAACAAAA TATGGATCTA 
ACCAAGAGTC ACACTGTTGT CATTGGATTT AGTCCTTCTG AAATACAAGA ATTGGGTAAG 
ACTAATCTAG AAAATACTAA CAGTCTGCTA ACAACAGCAA ACAGACAGAC AGCTATAAAA 
GTTGAAAAAT GTTGTAAAAG TCCTATAGAA AAAACAGGAA TATCTGTTTC TAAGGATAAC 
CTAGATGTGA TAGAGGACAA TAGCATACAG AAACTTGGAT TTTTGAATGA AAAGCAAGAT 
GTCAAAATTT GTGGAAGAAA AAGTGTTGGC AGACAAAAAG TTGATAAGAC TATTGTATTT 
TCAGAGGGTG ATGAGAATGA TATGGATATC ACCAAGATTT TTACAGTGGA AATAAATCAT 
AAACCTTTAT TACTTGAACA AGATTCCCAT TTGGCAGGAA CTTCTAAAAC TGTTTTGTAT 
GAATGTGGGC AAGATGATGT GGAGATCACT AAAAGTCACA CAACTGCCTT GGAATGTATA 
ACTGTCTCAC CAGATAAAAT AACCAGTAGG CCTATGGACA AAACTGTAAT GTTTGTAGAT 
AATTACAATG AACTGGAAAT GACAAAGTCT CACACTGTTT TCATTGACTA CCAAGCAAAG 
GAGAGGACTG TGAGTATAGA CAAACTTGAC ACGGAACTAT CCAAAAGAAG AAAAAGCATA 
GAAAAGCCAA AAATGACACC TGCTTCTGCT GAGGAGAGTG TTTCCTTTCT GGAGAATGGT 
GAAAGTGACC ATTCAGCAGC AAACGTCAAT AAGCTAACAC TACTGAGGGA ATGGTCTAAT 
AATGGTCCTA TAGAGAAAGC AGTAGCTGGT GATAACATGG AAGTATCTAA ATTCACCATT 
TGGAAAAATG GCAAAGATGT ACAAAAGCCT GAATTTCTGA ATGACACTAT ATCAGACAAA 
AGTCAGAGAA GGAAAAGCCT TAGACTCAAA AATGACAAGA TTGCTGCATT TTCTAAAAAT 
GATGATGATA TGGATATCAC CCAGAGTTGT ACAGTGAAAA TAAACTATAA AAGTGCCCCA 
GAAGATAGAG AGGATTCCCA TTTGGTGCCT TTGACAGGAG CTTCTAAAAC TGTTTTGTAT 
TCATGTGGGC AGGATGACAT GGAGATGACT AGAAGTCATA CGACTGCGTT AGAATGTAAA 
ACTGTCTCAC CGGATAAAAT CACTACTAGG CCCATAGATA AAACTGTAAT GTTTGTTGAT 
AATCATGGTG ATCTGGAAGT CACCGAGTCC CATACTGTTT TCATTGATTG TCAAAACACA 
GAGAAAATTA ATCAAGAGTG CCCTAAATTT GGAATAGCTA CAGGAAAACC CTTGGGTATT 
TCTTTTCATA ATGATGGTAG CTCTGTTCAA GAAATCACTG AAAAACAAGC ATTGGCAGTA 
GACCAGACCT ATATACCGGA AAAAGCCAGA ATTGGAAGCT GTCAGTTAAA TAGTACAGAT 
AGAAGAAACA TGGACTTTAC TAACAGTCAA GCAACTGCTA CGTGTGGATC CAGTGATAAT 
TATTCCTGTT TACCAAATGC TATTTCCTGT TTTGATAATT TAGAGGGGAA TGTCATGTCC 
TTAAGTAATA AAGATAAGAA AGCCAGTAAC TGCCCAGTGC AAAATGATCT TTCTTATGTA 
AAGGATTTAG CCAATGAATA TTACTTGAAA CCTGAGGGAC TGCCTCCCTC TGCTTGTTCT 
TTGTTAAAGA AAGAAAAAGT GACTCAAACC AATACCAATG GACAGTTAGA CTGTGTTACA 
ACAGTGCTTA AAGATCAAGA TATGATTAAG GAGTCCCAGA ATCTAATAGC TAATCAAACT 
TTACAAGATA GTCAGGAGCT GGCGGAGATG ATTAAACCTA ATTCAAATCG AGTATCCTTT 
AAACCCCCAA AGGATCAAAT GGAGGGCTTT GTTGATACCA CGGAACATAA TTTAAAGAAG 
ACTGTTGTTG ATAATGTTTG TGTTACTTCT CAGCCTCATC TCACAAGCCA GGTACCTCCA 
TTACCTCAAC AAGGACGGAT AATTTTGAAT AAAGATGAAA CAATATCATC TAATTCTGGA 
AATAAGAATT TAAATATTGT TATAGGAAAT TCCTTTGTAC CCACATGTGA AAACGAGGCC 
AAAATGCTCA ACAATGATAA ACAATTTACC ATAGTCTGTA AAAATGAGCT GAGGAAAAAT 
ACTAAATGTA ATACCGATTT CCACAGTAAT TCAGACTTGA CTAAGCAAGT TATTCAAACT 
CATACTAATT CTAGAGAAGC ATCAGATACT GTAATTGCAT CTAATACAAG TTTTAATAGT 
GTCAGATCAA ATCTGAATAA TTTGAATGGA AAAACTGAAG AATTTTTAGA TTTCCAAACT 
CCCCATATTC CATCTTCTCC AGAGCAAATA CTTGCATTAA TAAACAAGGC ACACAATCAT 
ATGAGTATAT TGCAAGCTAC AGAAATACAG AACATTAACC CAGCGCCCAG CAATGTTGAA 
GATGATAAAG ATGAAGAAAG CAAAATTTCT CATAATGGAG CTGAAACCAA CTCTGTACCA 
CTCATGACAG TTGTAAAAGA TAAAGTGAAG AGATGTTCTT TGGGAATATT TTTGCCCAGA 
TTGCCAAACA AGAGAAATTG TAGTGTCACT GGTATTAATG ACCTGAAGCA GATTCAAGGA 
GACACAACTG ATTTAAATCA CTTAGAAATT CAGCCAGTCT TCAGTAAGGA GCCAAGCATT 
GAATTTGTTG CAACTAAACT GACCTTAAGT CCATCTCAGT ATATAAATGA GGAAAACTTT 
CCTATATATC CTGGTGAGAT TAATTCCTCA GATTCTATTA CCATAGAAAC TGAGGAAAAG 
GCTTTGATTG AGACATATCA AAAAGGGATT CCACCATCTG AAAATAAGAT AAGAGAAACC 
TGCAGTAACC AAAAAAGAAC CTGGGTACAA GAAGATGATG ATATTCAGAA TGAGAAAAAA 
ATCAGGAAAA GTGAGATTAG GTGTAGTGAT ACTACACAAG ATCAGGAGAT TTTTGATCAC 
CATGCTGAAG GGGATATAGA TAAAAATGCT AACAGCCTGA GCAGAACCCC ATCTAGTTGC 
AGCAGTTCTC TGGATTCGAT AAAGGCTGAT GGGACCTCTC TGGACTTCAG CACACAGCAC 
AATAGTCAAA TGGAATCACA GTTTCTCAGA GACACTATTT GTGAAGAGAG CTTGAAGGAG 
AAACTCAAAG ATGGGAAAAT TACAATAAAG GAGTTCTTTA TACTTCTTCA GGTCCACATT 
TTGATACAGA AACCCCGACA GAGCAATCTC CCAGCCAAAT TTACTATAAA CACACCACCT 
ACTCTGGAAG ACCTGATGTT AAGTCAGTAT GTTTACCGAC CCAAGATACA GATTTATAGA 
GAAGATTGTG AAGCTCTTTG CCAAAAGATT GAAGAATTAA AGCTTTCTGC ATTGAACCAG 
GATAAATTGT TGACTGACAT AAATAGGAAC CTGTGGGAAA AAATGAGACA CTGCTCTGAT 
GAAGAGCTGA AGGTCTTTGG AATTTACCTG AACAAAATAA AATCACATTT TACCAAGATG 
ACTAAAGTTT TTACTCACCA AGGAAAAGTG GCTCTCTATA CCAAGCTGGT ACAGTCAGCT 
CAGAATGAGA GGAAGAAACT TCAGATAAGG ATAGATGAGA TGGACAACAT ACTTAAGAAG 
ATTGAGAACT GTCTCGCTGA AGTTGAAATA GAAACTAAGA ATTTGGATGA AGAGAAAGAC 
AATCCTATGG AAGAATGGGA TTCTGAAATG AGAGCCGCAG AGAAAGAACT AGAACAGCTG 
AAAACTGAAG AAGATGAGCT CCAAAGAAAT CTCTTAGAAC TGGAAGTACA GAAAGAGCAG 
ACCCTTGCTC AAATAGACTT TGTACAAAAA CAAACAGATA GAACTGAAGA GCTAATGGAT 
CAGTTGAGCT TGTCTGAGTG GGAAGCCATT GAGTGGAGTG ATGATCAAGC TGTATTCACC 
TTTCTTTATG ACACAATAGA ACTTACTATC ACCTTTGGAG AGACAGTAGC TGGAAGTCCC 
AAGATCAGGC TGCCAGCATG GTCAGTTGAT CTCCCTTTCC TGGACAAGGC TTGTAGGAAG 
ATTGTTGACT TGAATTTTCA ATCTCGGTTA GATGAGGATA AAGCTCCTCC TTCCTCCCTT 
TTAGTTCATA AGCTTATTTT CCAGTACATT GAGGAAAAGG AATCCTGGAA GAAAAAATGT 
ACAACACAGC ATCAAATACC CAAGATGCTT CAAGAAATCT CATTGGTAGT GAGCCATTGT 
AGACTCCTTG GAGAGGAAAT TGAGTTTTTA AAGAGATGGG GACCAAATTA TAACCTAATG 
AACATAGATG TGACTAATAC TGAATTGAGG CTTTTATTCT CCAGCTCTGC AGCATTTGCA 
AAATTTGAGA TAACTTTGCC TCTCTCAGTC CATTTTCCAA CTGTTCCATT ACCTTTCTCC 
ATTCAAAAGC ACCTTGGAAA CATTGGCCAA GATGAAATCA CTGCAATTCT ATCTAAAGTG 
CCACTGAAGA ACAACTATCT AAAGAATGTA GTCAAGCAAA TTTACCAAGA TCTGCTTCAG 
GACTGCCATT TCTATCACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KNL1 ( XM_005638256.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005638313.1 Canis lupus familiaris kinetochore scaffold 1 (KNL1), transcript variant X3, mRNA.

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CloneID	OCe28662
Clone ID Related Accession (Same CDS sequence)	XM_005638256.2 , XM_005638256.1
Accession Version	XM_005638256.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6825bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	kinetochore scaffold 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006612.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005638256.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGGGA TGTCTTCAGA GACTAATGAA GAGAATGACA ATATGGAGAG ACCTGTTAGA 
AGACGACATT CCTCCATTTT GAAACCCCCA AGGAGTCCTC TTCAGGACCT CAGAGGTGGG 
AATGAAGCAG TTCAGGAATC TAATGCTTTG AGAAATAAGA AAAGCTCTCG TCGAGTCAGC 
TTTGCAGATA CCATAAAAGT ATTCCAAACA GAGTCTCATA TGAAAATAGT AAGAAAGTCA 
GAAATTGCAG AAACAGAAGC AGGAGAAAAT GTTCTATATA TACAGAATAA GAAGCCAGAA 
GAAACCTACT GTGAAATTAC TGGGATGAAC ACATTGCTTT CTGCTCCTAT TCAGACTCAG 
ATGCAACAGA GAGAGATTTC AATTACAGAA CACAACCATG AAATGAAACC TGCAAATGAC 
CAAACAGTCA TCTTTTCAGA TGAAAACCAG ATGGACCTAA CAGCAAGTCA CACCGTAATG 
ATTACCAAAG ACCTGTTAGA TTGTACTAAA AGTGAAAAGT CCATTAAGAT AGATACCGCA 
TCATTTCTGG CTAACTTAAA GCTCCATACT AAGGATTCAA GAATGAAAAA AGAATTAAAT 
TTTTCCATGG ATCAAAGCAC TTCTTCAGAA AAGAAAATAC ATTTTAATGA CTTCATAAAA 
AGATTGAAAA CAGGAAAATC TAATGCTTTT CCTACAGGAC CTGATAAAGA AAATTTTGAA 
ATATCTGTTC ATCCCATAAA AACAAAGAAG GCCTCTTCTG TACATCAAAT GCATGTATCT 
CTTAGGGTAG ATGAAAATAG TAGTAATACA ACTAGAATCT TCGGAGAACA AAATGATGGG 
ATGAATTTCA CTCAGTGTCA TACAGCCAGT ATTCAGACTT TGGTTCCCAC ATCCAGTAAG 
GCCAGCATAG GGGAATTTAA AGGTGATGAT ATTACAATTT ATGGCAGTGA TTTTATGGAC 
TTGACAACTA ACCACACTAT ACAGAATTTA CCCTCAGCAG ATAATTCATC TAATAAAGAA 
AATCAAACTC AGAATGTCAT GATGGAGGTT AAAGCTCCAG GAGGGAAGAC AATCTTTAAT 
AAACTGAATA TTCCTTTTCA AACCCCTTTA AACCCTAATG GTGAAATACA TATTACCAAA 
AGTCATATTA CAGGGACAGA AACTCACACA AGCACACAGA CTTCTAACCA AGATGCCAGA 
ACATTGACTA TAATCCCAGA ATCTATATGT TCCAGTCCAG CTACTCAAGA TTATAAGACA 
TTTTTCTATT CTAGCAGTAA TAATGCCATG GAACTGACCA CGTGTCTCTC GGGTATGAGA 
AAAGAGAAGA ATTTGCTGAA GAATGATAAT TATTCTAAAA TGTATCCCAA TCCAGATGCC 
ATCTCTCTTC TTACAGAGAA AACTATTTAT TCAGGAGAGG ATAGCATGGA CATTACGAGG 
AGTCATACAG TTGCAATAGA TAATCAGATT TTTAAACAAG ATCAGACAAA TATACAGAAA 
GCAGACACAT CAATATCTGA AAAAGAAATA ATGCTCCAAA ATCTCATAAC CATGTCAAAG 
GACGGGAAAA TGAATATAAA TTGTAGCTCA GTTCCTCATA TATCTAAGGG AAGATTACAG 
CAGAGCCTGG CAAACTCTTT ATCTATTTCA TTGACTGACA AAAAGACTGA AATCTTCACA 
GGTGAAGATA TGGATTTGAC TAAAAGTCAC ACAACTAACT TAGGAAGTCA AGCTCCACTT 
ACATCTTACA ATGTAACATC TGAGAATACC AGTAAATTTC TCTCTCACAG CAAAAGCCCT 
TCAGGTGAAT GGCAAGAAAT GACCAAAAGT CGTATTGAAC CATCCCAGCA GCCAGACATA 
ATATCTAAAA ACATCCCAGT GGACACCTGG GGCAAAGAAA AAGTTCAAGT TTTAGAGATT 
GCACCTTATG TTGATAAAGA TTCTCCTCAA TCAGCTGATA TTAATCAAGA CATTGCAATA 
AGCCATAGTA TAGTATACTC TGATAGAAAT CTTGAGAAAC AAGTAGCACT AGGAAATAAT 
AGAAATACTG TTCCATGTGA GCAATCTTTG TTTCCTACTT CAGAGTCATT ATTTTCATTA 
GAAGGACAGT CTAACATGAA AAATCATAAT ATTGCTATTA ACAGTTACAC AATGAAATCT 
GTGCCAGGCC AGAATTCTAA ACTGCCTGAG CCACTGAGGA AAGGTGTAGG CAATCCAACA 
CCTGACTATT CTCATGACAA AACAATTATT TGTTCAGAGG AGGAACAAAA TATGGATCTA 
ACCAAGAGTC ACACTGTTGT CATTGGATTT AGTCCTTCTG AAATACAAGA ATTGGGTAAG 
ACTAATCTAG AAAATACTAA CAGTCTGCTA ACAACAGCAA ACAGACAGAC AGCTATAAAA 
GTTGAAAAAT GTTGTAAAAG TCCTATAGAA AAAACAGGAA TATCTGTTTC TAAGGATAAC 
CTAGATGTGA TAGAGGACAA TAGCATACAG AAACTTGGAT TTTTGAATGA AAAGCAAGAT 
GTCAAAATTT GTGGAAGAAA AAGTGTTGGC AGACAAAAAG TTGATAAGAC TATTGTATTT 
TCAGAGGGTG ATGAGAATGA TATGGATATC ACCAAGATTT TTACAGTGGA AATAAATCAT 
AAACCTTTAT TACTTGAACA AGATTCCCAT TTGGCAGGAA CTTCTAAAAC TGTTTTGTAT 
GAATGTGGGC AAGATGATGT GGAGATCACT AAAAGTCACA CAACTGCCTT GGAATGTATA 
ACTGTCTCAC CAGATAAAAT AACCAGTAGG CCTATGGACA AAACTGTAAT GTTTGTAGAT 
AATTACAATG AACTGGAAAT GACAAAGTCT CACACTGTTT TCATTGACTA CCAAGCAAAG 
GAGAGGACTG TGAGTATAGA CAAACTTGAC ACGGAACTAT CCAAAAGAAG AAAAAGCATA 
GAAAAGCCAA AAATGACACC TGCTTCTGCT GAGGAGAGTG TTTCCTTTCT GGAGAATGGT 
GAAAGTGACC ATTCAGCAGC AAACGTCAAT AAGCTAACAC TACTGAGGGA ATGGTCTAAT 
AATGGTCCTA TAGAGAAAGC AGTAGCTGGT GATAACATGG AAGTATCTAA ATTCACCATT 
TGGAAAAATG GCAAAGATGT ACAAAAGCCT GAATTTCTGA ATGACACTAT ATCAGACAAA 
AGTCAGAGAA GGAAAAGCCT TAGACTCAAA AATGACAAGA TTGCTGCATT TTCTAAAAAT 
GATGATGATA TGGATATCAC CCAGAGTTGT ACAGTGAAAA TAAACTATAA AAGTGCCCCA 
GAAGATAGAG AGGATTCCCA TTTGGTGCCT TTGACAGGAG CTTCTAAAAC TGTTTTGTAT 
TCATGTGGGC AGGATGACAT GGAGATGACT AGAAGTCATA CGACTGCGTT AGAATGTAAA 
ACTGTCTCAC CGGATAAAAT CACTACTAGG CCCATAGATA AAACTGTAAT GTTTGTTGAT 
AATCATGGTG ATCTGGAAGT CACCGAGTCC CATACTGTTT TCATTGATTG TCAAAACACA 
GAGAAAATTA ATCAAGAGTG CCCTAAATTT GGAATAGCTA CAGGAAAACC CTTGGGTATT 
TCTTTTCATA ATGATGGTAG CTCTGTTCAA GAAATCACTG AAAAACAAGC ATTGGCAGTA 
GACCAGACCT ATATACCGGA AAAAGCCAGA ATTGGAAGCT GTCAGTTAAA TAGTACAGAT 
AGAAGAAACA TGGACTTTAC TAACAGTCAA GCAACTGCTA CGTGTGGATC CAGTGATAAT 
TATTCCTGTT TACCAAATGC TATTTCCTGT TTTGATAATT TAGAGGGGAA TGTCATGTCC 
TTAAGTAATA AAGATAAGAA AGCCAGTAAC TGCCCAGTGC AAAATGATCT TTCTTATGTA 
AAGGATTTAG CCAATGAATA TTACTTGAAA CCTGAGGGAC TGCCTCCCTC TGCTTGTTCT 
TTGTTAAAGA AAGAAAAAGT GACTCAAACC AATACCAATG GACAGTTAGA CTGTGTTACA 
ACAGTGCTTA AAGATCAAGA TATGATTAAG GAGTCCCAGA ATCTAATAGC TAATCAAACT 
TTACAAGATA GTCAGGAGCT GGCGGAGATG ATTAAACCTA ATTCAAATCG AGTATCCTTT 
AAACCCCCAA AGGATCAAAT GGAGGGCTTT GTTGATACCA CGGAACATAA TTTAAAGAAG 
ACTGTTGTTG ATAATGTTTG TGTTACTTCT CAGCCTCATC TCACAAGCCA GGTACCTCCA 
TTACCTCAAC AAGGACGGAT AATTTTGAAT AAAGATGAAA CAATATCATC TAATTCTGGA 
AATAAGAATT TAAATATTGT TATAGGAAAT TCCTTTGTAC CCACATGTGA AAACGAGGCC 
AAAATGCTCA ACAATGATAA ACAATTTACC ATAGTCTGTA AAAATGAGCT GAGGAAAAAT 
ACTAAATGTA ATACCGATTT CCACAGTAAT TCAGACTTGA CTAAGCAAGT TATTCAAACT 
CATACTAATT CTAGAGAAGC ATCAGATACT GTAATTGCAT CTAATACAAG TTTTAATAGT 
GTCAGATCAA ATCTGAATAA TTTGAATGGA AAAACTGAAG AATTTTTAGA TTTCCAAACT 
CCCCATATTC CATCTTCTCC AGAGCAAATA CTTGCATTAA TAAACAAGGC ACACAATCAT 
ATGAGTATAT TGCAAGCTAC AGAAATACAG AACATTAACC CAGCGCCCAG CAATGTTGAA 
GATGATAAAG ATGAAGAAAG CAAAATTTCT CATAATGGAG CTGAAACCAA CTCTGTACCA 
CTCATGACAG TTGTAAAAGA TAAAGTGAAG AGATGTTCTT TGGGAATATT TTTGCCCAGA 
TTGCCAAACA AGAGAAATTG TAGTGTCACT GGTATTAATG ACCTGAAGCA GATTCAAGGA 
GACACAACTG ATTTAAATCA CTTAGAAATT CAGCCAGTCT TCAGTAAGGA GCCAAGCATT 
GAATTTGTTG CAACTAAACT GACCTTAAGT CCATCTCAGT ATATAAATGA GGAAAACTTT 
CCTATATATC CTGGTGAGAT TAATTCCTCA GATTCTATTA CCATAGAAAC TGAGGAAAAG 
GCTTTGATTG AGACATATCA AAAAGGGATT CCACCATCTG AAAATAAGAT AAGAGAAACC 
TGCAGTAACC AAAAAAGAAC CTGGGTACAA GAAGATGATG ATATTCAGAA TGAGAAAAAA 
ATCAGGAAAA GTGAGATTAG GTGTAGTGAT ACTACACAAG ATCAGGAGAT TTTTGATCAC 
CATGCTGAAG GGGATATAGA TAAAAATGCT AACAGCCTGA GCAGAACCCC ATCTAGTTGC 
AGCAGTTCTC TGGATTCGAT AAAGGCTGAT GGGACCTCTC TGGACTTCAG CACACAGCAC 
AATAGTCAAA TGGAATCACA GTTTCTCAGA GACACTATTT GTGAAGAGAG CTTGAAGGAG 
AAACTCAAAG ATGGGAAAAT TACAATAAAG GAGTTCTTTA TACTTCTTCA GGTCCACATT 
TTGATACAGA AACCCCGACA GAGCAATCTC CCAGCCAAAT TTACTATAAA CACACCACCT 
ACTCTGGAAG ACCTGATGTT AAGTCAGTAT GTTTACCGAC CCAAGATACA GATTTATAGA 
GAAGATTGTG AAGCTCTTTG CCAAAAGATT GAAGAATTAA AGCTTTCTGC ATTGAACCAG 
GATAAATTGT TGACTGACAT AAATAGGAAC CTGTGGGAAA AAATGAGACA CTGCTCTGAT 
GAAGAGCTGA AGGTCTTTGG AATTTACCTG AACAAAATAA AATCACATTT TACCAAGATG 
ACTAAAGTTT TTACTCACCA AGGAAAAGTG GCTCTCTATA CCAAGCTGGT ACAGTCAGCT 
CAGAATGAGA GGAAGAAACT TCAGATAAGG ATAGATGAGA TGGACAACAT ACTTAAGAAG 
ATTGAGAACT GTCTCGCTGA AGTTGAAATA GAAACTAAGA ATTTGGATGA AGAGAAAGAC 
AATCCTATGG AAGAATGGGA TTCTGAAATG AGAGCCGCAG AGAAAGAACT AGAACAGCTG 
AAAACTGAAG AAGATGAGCT CCAAAGAAAT CTCTTAGAAC TGGAAGTACA GAAAGAGCAG 
ACCCTTGCTC AAATAGACTT TGTACAAAAA CAAACAGATA GAACTGAAGA GCTAATGGAT 
CAGTTGAGCT TGTCTGAGTG GGAAGCCATT GAGTGGAGTG ATGATCAAGC TGTATTCACC 
TTTCTTTATG ACACAATAGA ACTTACTATC ACCTTTGGAG AGACAGTAGT TGATCTCCCT 
TTCCTGGACA AGGCTTGTAG GAAGATTGTT GACTTGAATT TTCAATCTCG GTTAGATGAG 
GATAAAGCTC CTCCTTCCTC CCTTTTAGTT CATAAGCTTA TTTTCCAGTA CATTGAGGAA 
AAGGAATCCT GGAAGAAAAA ATGTACAACA CAGCATCAAA TACCCAAGAT GCTTCAAGAA 
ATCTCATTGG TAGTGAGCCA TTGTAGACTC CTTGGAGAGG AAATTGAGTT TTTAAAGAGA 
TGGGGACCAA ATTATAACCT AATGAACATA GATGTGACTA ATACTGAATT GAGGCTTTTA 
TTCTCCAGCT CTGCAGCATT TGCAAAATTT GAGATAACTT TGCCTCTCTC AGTCCATTTT 
CCAACTGTTC CATTACCTTT CTCCATTCAA AAGCACCTTG GAAACATTGG CCAAGATGAA 
ATCACTGCAA TTCTATCTAA AGTGCCACTG AAGAACAACT ATCTAAAGAA TGTAGTCAAG 
CAAATTTACC AAGATCTGCT TCAGGACTGC CATTTCTATC ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005638313.1
CDS	338..7162
Translation	MDGMSSETNEENDNMERPVRRRHSSILKPPRSPLQDLRGGNEAVQESNALRNKKSSRRVSFADTIKVFQTESHMKIVRKSEIAETEAGENVLYIQNKKPEETYCEITGMNTLLSAPIQTQMQQREISITEHNHEMKPANDQTVIFSDENQMDLTASHTVMITKDLLDCTKSEKSIKIDTASFLANLKLHTKDSRMKKELNFSMDQSTSSEKKIHFNDFIKRLKTGKSNAFPTGPDKENFEISVHPIKTKKASSVHQMHVSLRVDENSSNTTRIFGEQNDGMNFTQCHTASIQTLVPTSSKASIGEFKGDDITIYGSDFMDLTTNHTIQNLPSADNSSNKENQTQNVMMEVKAPGGKTIFNKLNIPFQTPLNPNGEIHITKSHITGTETHTSTQTSNQDARTLTIIPESICSSPATQDYKTFFYSSSNNAMELTTCLSGMRKEKNLLKNDNYSKMYPNPDAISLLTEKTIYSGEDSMDITRSHTVAIDNQIFKQDQTNIQKADTSISEKEIMLQNLITMSKDGKMNINCSSVPHISKGRLQQSLANSLSISLTDKKTEIFTGEDMDLTKSHTTNLGSQAPLTSYNVTSENTSKFLSHSKSPSGEWQEMTKSRIEPSQQPDIISKNIPVDTWGKEKVQVLEIAPYVDKDSPQSADINQDIAISHSIVYSDRNLEKQVALGNNRNTVPCEQSLFPTSESLFSLEGQSNMKNHNIAINSYTMKSVPGQNSKLPEPLRKGVGNPTPDYSHDKTIICSEEEQNMDLTKSHTVVIGFSPSEIQELGKTNLENTNSLLTTANRQTAIKVEKCCKSPIEKTGISVSKDNLDVIEDNSIQKLGFLNEKQDVKICGRKSVGRQKVDKTIVFSEGDENDMDITKIFTVEINHKPLLLEQDSHLAGTSKTVLYECGQDDVEITKSHTTALECITVSPDKITSRPMDKTVMFVDNYNELEMTKSHTVFIDYQAKERTVSIDKLDTELSKRRKSIEKPKMTPASAEESVSFLENGESDHSAANVNKLTLLREWSNNGPIEKAVAGDNMEVSKFTIWKNGKDVQKPEFLNDTISDKSQRRKSLRLKNDKIAAFSKNDDDMDITQSCTVKINYKSAPEDREDSHLVPLTGASKTVLYSCGQDDMEMTRSHTTALECKTVSPDKITTRPIDKTVMFVDNHGDLEVTESHTVFIDCQNTEKINQECPKFGIATGKPLGISFHNDGSSVQEITEKQALAVDQTYIPEKARIGSCQLNSTDRRNMDFTNSQATATCGSSDNYSCLPNAISCFDNLEGNVMSLSNKDKKASNCPVQNDLSYVKDLANEYYLKPEGLPPSACSLLKKEKVTQTNTNGQLDCVTTVLKDQDMIKESQNLIANQTLQDSQELAEMIKPNSNRVSFKPPKDQMEGFVDTTEHNLKKTVVDNVCVTSQPHLTSQVPPLPQQGRIILNKDETISSNSGNKNLNIVIGNSFVPTCENEAKMLNNDKQFTIVCKNELRKNTKCNTDFHSNSDLTKQVIQTHTNSREASDTVIASNTSFNSVRSNLNNLNGKTEEFLDFQTPHIPSSPEQILALINKAHNHMSILQATEIQNINPAPSNVEDDKDEESKISHNGAETNSVPLMTVVKDKVKRCSLGIFLPRLPNKRNCSVTGINDLKQIQGDTTDLNHLEIQPVFSKEPSIEFVATKLTLSPSQYINEENFPIYPGEINSSDSITIETEEKALIETYQKGIPPSENKIRETCSNQKRTWVQEDDDIQNEKKIRKSEIRCSDTTQDQEIFDHHAEGDIDKNANSLSRTPSSCSSSLDSIKADGTSLDFSTQHNSQMESQFLRDTICEESLKEKLKDGKITIKEFFILLQVHILIQKPRQSNLPAKFTINTPPTLEDLMLSQYVYRPKIQIYREDCEALCQKIEELKLSALNQDKLLTDINRNLWEKMRHCSDEELKVFGIYLNKIKSHFTKMTKVFTHQGKVALYTKLVQSAQNERKKLQIRIDEMDNILKKIENCLAEVEIETKNLDEEKDNPMEEWDSEMRAAEKELEQLKTEEDELQRNLLELEVQKEQTLAQIDFVQKQTDRTEELMDQLSLSEWEAIEWSDDQAVFTFLYDTIELTITFGETVVDLPFLDKACRKIVDLNFQSRLDEDKAPPSSLLVHKLIFQYIEEKESWKKKCTTQHQIPKMLQEISLVVSHCRLLGEEIEFLKRWGPNYNLMNIDVTNTELRLLFSSSAAFAKFEITLPLSVHFPTVPLPFSIQKHLGNIGQDEITAILSKVPLKNNYLKNVVKQIYQDLLQDCHFYH

Target ORF information:

RefSeq Version	XM_005638256.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris kinetochore scaffold 1 (KNL1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005638256.2

ORF Insert Sequence:

ATGGATGGGA TGTCTTCAGA GACTAATGAA GAGAATGACA ATATGGAGAG ACCTGTTAGA 
AGACGACATT CCTCCATTTT GAAACCCCCA AGGAGTCCTC TTCAGGACCT CAGAGGTGGG 
AATGAAGCAG TTCAGGAATC TAATGCTTTG AGAAATAAGA AAAGCTCTCG TCGAGTCAGC 
TTTGCAGATA CCATAAAAGT ATTCCAAACA GAGTCTCATA TGAAAATAGT AAGAAAGTCA 
GAAATTGCAG AAACAGAAGC AGGAGAAAAT GTTCTATATA TACAGAATAA GAAGCCAGAA 
GAAACCTACT GTGAAATTAC TGGGATGAAC ACATTGCTTT CTGCTCCTAT TCAGACTCAG 
ATGCAACAGA GAGAGATTTC AATTACAGAA CACAACCATG AAATGAAACC TGCAAATGAC 
CAAACAGTCA TCTTTTCAGA TGAAAACCAG ATGGACCTAA CAGCAAGTCA CACCGTAATG 
ATTACCAAAG ACCTGTTAGA TTGTACTAAA AGTGAAAAGT CCATTAAGAT AGATACCGCA 
TCATTTCTGG CTAACTTAAA GCTCCATACT AAGGATTCAA GAATGAAAAA AGAATTAAAT 
TTTTCCATGG ATCAAAGCAC TTCTTCAGAA AAGAAAATAC ATTTTAATGA CTTCATAAAA 
AGATTGAAAA CAGGAAAATC TAATGCTTTT CCTACAGGAC CTGATAAAGA AAATTTTGAA 
ATATCTGTTC ATCCCATAAA AACAAAGAAG GCCTCTTCTG TACATCAAAT GCATGTATCT 
CTTAGGGTAG ATGAAAATAG TAGTAATACA ACTAGAATCT TCGGAGAACA AAATGATGGG 
ATGAATTTCA CTCAGTGTCA TACAGCCAGT ATTCAGACTT TGGTTCCCAC ATCCAGTAAG 
GCCAGCATAG GGGAATTTAA AGGTGATGAT ATTACAATTT ATGGCAGTGA TTTTATGGAC 
TTGACAACTA ACCACACTAT ACAGAATTTA CCCTCAGCAG ATAATTCATC TAATAAAGAA 
AATCAAACTC AGAATGTCAT GATGGAGGTT AAAGCTCCAG GAGGGAAGAC AATCTTTAAT 
AAACTGAATA TTCCTTTTCA AACCCCTTTA AACCCTAATG GTGAAATACA TATTACCAAA 
AGTCATATTA CAGGGACAGA AACTCACACA AGCACACAGA CTTCTAACCA AGATGCCAGA 
ACATTGACTA TAATCCCAGA ATCTATATGT TCCAGTCCAG CTACTCAAGA TTATAAGACA 
TTTTTCTATT CTAGCAGTAA TAATGCCATG GAACTGACCA CGTGTCTCTC GGGTATGAGA 
AAAGAGAAGA ATTTGCTGAA GAATGATAAT TATTCTAAAA TGTATCCCAA TCCAGATGCC 
ATCTCTCTTC TTACAGAGAA AACTATTTAT TCAGGAGAGG ATAGCATGGA CATTACGAGG 
AGTCATACAG TTGCAATAGA TAATCAGATT TTTAAACAAG ATCAGACAAA TATACAGAAA 
GCAGACACAT CAATATCTGA AAAAGAAATA ATGCTCCAAA ATCTCATAAC CATGTCAAAG 
GACGGGAAAA TGAATATAAA TTGTAGCTCA GTTCCTCATA TATCTAAGGG AAGATTACAG 
CAGAGCCTGG CAAACTCTTT ATCTATTTCA TTGACTGACA AAAAGACTGA AATCTTCACA 
GGTGAAGATA TGGATTTGAC TAAAAGTCAC ACAACTAACT TAGGAAGTCA AGCTCCACTT 
ACATCTTACA ATGTAACATC TGAGAATACC AGTAAATTTC TCTCTCACAG CAAAAGCCCT 
TCAGGTGAAT GGCAAGAAAT GACCAAAAGT CGTATTGAAC CATCCCAGCA GCCAGACATA 
ATATCTAAAA ACATCCCAGT GGACACCTGG GGCAAAGAAA AAGTTCAAGT TTTAGAGATT 
GCACCTTATG TTGATAAAGA TTCTCCTCAA TCAGCTGATA TTAATCAAGA CATTGCAATA 
AGCCATAGTA TAGTATACTC TGATAGAAAT CTTGAGAAAC AAGTAGCACT AGGAAATAAT 
AGAAATACTG TTCCATGTGA GCAATCTTTG TTTCCTACTT CAGAGTCATT ATTTTCATTA 
GAAGGACAGT CTAACATGAA AAATCATAAT ATTGCTATTA ACAGTTACAC AATGAAATCT 
GTGCCAGGCC AGAATTCTAA ACTGCCTGAG CCACTGAGGA AAGGTGTAGG CAATCCAACA 
CCTGACTATT CTCATGACAA AACAATTATT TGTTCAGAGG AGGAACAAAA TATGGATCTA 
ACCAAGAGTC ACACTGTTGT CATTGGATTT AGTCCTTCTG AAATACAAGA ATTGGGTAAG 
ACTAATCTAG AAAATACTAA CAGTCTGCTA ACAACAGCAA ACAGACAGAC AGCTATAAAA 
GTTGAAAAAT GTTGTAAAAG TCCTATAGAA AAAACAGGAA TATCTGTTTC TAAGGATAAC 
CTAGATGTGA TAGAGGACAA TAGCATACAG AAACTTGGAT TTTTGAATGA AAAGCAAGAT 
GTCAAAATTT GTGGAAGAAA AAGTGTTGGC AGACAAAAAG TTGATAAGAC TATTGTATTT 
TCAGAGGGTG ATGAGAATGA TATGGATATC ACCAAGATTT TTACAGTGGA AATAAATCAT 
AAACCTTTAT TACTTGAACA AGATTCCCAT TTGGCAGGAA CTTCTAAAAC TGTTTTGTAT 
GAATGTGGGC AAGATGATGT GGAGATCACT AAAAGTCACA CAACTGCCTT GGAATGTATA 
ACTGTCTCAC CAGATAAAAT AACCAGTAGG CCTATGGACA AAACTGTAAT GTTTGTAGAT 
AATTACAATG AACTGGAAAT GACAAAGTCT CACACTGTTT TCATTGACTA CCAAGCAAAG 
GAGAGGACTG TGAGTATAGA CAAACTTGAC ACGGAACTAT CCAAAAGAAG AAAAAGCATA 
GAAAAGCCAA AAATGACACC TGCTTCTGCT GAGGAGAGTG TTTCCTTTCT GGAGAATGGT 
GAAAGTGACC ATTCAGCAGC AAACGTCAAT AAGCTAACAC TACTGAGGGA ATGGTCTAAT 
AATGGTCCTA TAGAGAAAGC AGTAGCTGGT GATAACATGG AAGTATCTAA ATTCACCATT 
TGGAAAAATG GCAAAGATGT ACAAAAGCCT GAATTTCTGA ATGACACTAT ATCAGACAAA 
AGTCAGAGAA GGAAAAGCCT TAGACTCAAA AATGACAAGA TTGCTGCATT TTCTAAAAAT 
GATGATGATA TGGATATCAC CCAGAGTTGT ACAGTGAAAA TAAACTATAA AAGTGCCCCA 
GAAGATAGAG AGGATTCCCA TTTGGTGCCT TTGACAGGAG CTTCTAAAAC TGTTTTGTAT 
TCATGTGGGC AGGATGACAT GGAGATGACT AGAAGTCATA CGACTGCGTT AGAATGTAAA 
ACTGTCTCAC CGGATAAAAT CACTACTAGG CCCATAGATA AAACTGTAAT GTTTGTTGAT 
AATCATGGTG ATCTGGAAGT CACCGAGTCC CATACTGTTT TCATTGATTG TCAAAACACA 
GAGAAAATTA ATCAAGAGTG CCCTAAATTT GGAATAGCTA CAGGAAAACC CTTGGGTATT 
TCTTTTCATA ATGATGGTAG CTCTGTTCAA GAAATCACTG AAAAACAAGC ATTGGCAGTA 
GACCAGACCT ATATACCGGA AAAAGCCAGA ATTGGAAGCT GTCAGTTAAA TAGTACAGAT 
AGAAGAAACA TGGACTTTAC TAACAGTCAA GCAACTGCTA CGTGTGGATC CAGTGATAAT 
TATTCCTGTT TACCAAATGC TATTTCCTGT TTTGATAATT TAGAGGGGAA TGTCATGTCC 
TTAAGTAATA AAGATAAGAA AGCCAGTAAC TGCCCAGTGC AAAATGATCT TTCTTATGTA 
AAGGATTTAG CCAATGAATA TTACTTGAAA CCTGAGGGAC TGCCTCCCTC TGCTTGTTCT 
TTGTTAAAGA AAGAAAAAGT GACTCAAACC AATACCAATG GACAGTTAGA CTGTGTTACA 
ACAGTGCTTA AAGATCAAGA TATGATTAAG GAGTCCCAGA ATCTAATAGC TAATCAAACT 
TTACAAGATA GTCAGGAGCT GGCGGAGATG ATTAAACCTA ATTCAAATCG AGTATCCTTT 
AAACCCCCAA AGGATCAAAT GGAGGGCTTT GTTGATACCA CGGAACATAA TTTAAAGAAG 
ACTGTTGTTG ATAATGTTTG TGTTACTTCT CAGCCTCATC TCACAAGCCA GGTACCTCCA 
TTACCTCAAC AAGGACGGAT AATTTTGAAT AAAGATGAAA CAATATCATC TAATTCTGGA 
AATAAGAATT TAAATATTGT TATAGGAAAT TCCTTTGTAC CCACATGTGA AAACGAGGCC 
AAAATGCTCA ACAATGATAA ACAATTTACC ATAGTCTGTA AAAATGAGCT GAGGAAAAAT 
ACTAAATGTA ATACCGATTT CCACAGTAAT TCAGACTTGA CTAAGCAAGT TATTCAAACT 
CATACTAATT CTAGAGAAGC ATCAGATACT GTAATTGCAT CTAATACAAG TTTTAATAGT 
GTCAGATCAA ATCTGAATAA TTTGAATGGA AAAACTGAAG AATTTTTAGA TTTCCAAACT 
CCCCATATTC CATCTTCTCC AGAGCAAATA CTTGCATTAA TAAACAAGGC ACACAATCAT 
ATGAGTATAT TGCAAGCTAC AGAAATACAG AACATTAACC CAGCGCCCAG CAATGTTGAA 
GATGATAAAG ATGAAGAAAG CAAAATTTCT CATAATGGAG CTGAAACCAA CTCTGTACCA 
CTCATGACAG TTGTAAAAGA TAAAGTGAAG AGATGTTCTT TGGGAATATT TTTGCCCAGA 
TTGCCAAACA AGAGAAATTG TAGTGTCACT GGTATTAATG ACCTGAAGCA GATTCAAGGA 
GACACAACTG ATTTAAATCA CTTAGAAATT CAGCCAGTCT TCAGTAAGGA GCCAAGCATT 
GAATTTGTTG CAACTAAACT GACCTTAAGT CCATCTCAGT ATATAAATGA GGAAAACTTT 
CCTATATATC CTGGTGAGAT TAATTCCTCA GATTCTATTA CCATAGAAAC TGAGGAAAAG 
GCTTTGATTG AGACATATCA AAAAGGGATT CCACCATCTG AAAATAAGAT AAGAGAAACC 
TGCAGTAACC AAAAAAGAAC CTGGGTACAA GAAGATGATG ATATTCAGAA TGAGAAAAAA 
ATCAGGAAAA GTGAGATTAG GTGTAGTGAT ACTACACAAG ATCAGGAGAT TTTTGATCAC 
CATGCTGAAG GGGATATAGA TAAAAATGCT AACAGCCTGA GCAGAACCCC ATCTAGTTGC 
AGCAGTTCTC TGGATTCGAT AAAGGCTGAT GGGACCTCTC TGGACTTCAG CACACAGCAC 
AATAGTCAAA TGGAATCACA GTTTCTCAGA GACACTATTT GTGAAGAGAG CTTGAAGGAG 
AAACTCAAAG ATGGGAAAAT TACAATAAAG GAGTTCTTTA TACTTCTTCA GGTCCACATT 
TTGATACAGA AACCCCGACA GAGCAATCTC CCAGCCAAAT TTACTATAAA CACACCACCT 
ACTCTGGAAG ACCTGATGTT AAGTCAGTAT GTTTACCGAC CCAAGATACA GATTTATAGA 
GAAGATTGTG AAGCTCTTTG CCAAAAGATT GAAGAATTAA AGCTTTCTGC ATTGAACCAG 
GATAAATTGT TGACTGACAT AAATAGGAAC CTGTGGGAAA AAATGAGACA CTGCTCTGAT 
GAAGAGCTGA AGGTCTTTGG AATTTACCTG AACAAAATAA AATCACATTT TACCAAGATG 
ACTAAAGTTT TTACTCACCA AGGAAAAGTG GCTCTCTATA CCAAGCTGGT ACAGTCAGCT 
CAGAATGAGA GGAAGAAACT TCAGATAAGG ATAGATGAGA TGGACAACAT ACTTAAGAAG 
ATTGAGAACT GTCTCGCTGA AGTTGAAATA GAAACTAAGA ATTTGGATGA AGAGAAAGAC 
AATCCTATGG AAGAATGGGA TTCTGAAATG AGAGCCGCAG AGAAAGAACT AGAACAGCTG 
AAAACTGAAG AAGATGAGCT CCAAAGAAAT CTCTTAGAAC TGGAAGTACA GAAAGAGCAG 
ACCCTTGCTC AAATAGACTT TGTACAAAAA CAAACAGATA GAACTGAAGA GCTAATGGAT 
CAGTTGAGCT TGTCTGAGTG GGAAGCCATT GAGTGGAGTG ATGATCAAGC TGTATTCACC 
TTTCTTTATG ACACAATAGA ACTTACTATC ACCTTTGGAG AGACAGTAGT TGATCTCCCT 
TTCCTGGACA AGGCTTGTAG GAAGATTGTT GACTTGAATT TTCAATCTCG GTTAGATGAG 
GATAAAGCTC CTCCTTCCTC CCTTTTAGTT CATAAGCTTA TTTTCCAGTA CATTGAGGAA 
AAGGAATCCT GGAAGAAAAA ATGTACAACA CAGCATCAAA TACCCAAGAT GCTTCAAGAA 
ATCTCATTGG TAGTGAGCCA TTGTAGACTC CTTGGAGAGG AAATTGAGTT TTTAAAGAGA 
TGGGGACCAA ATTATAACCT AATGAACATA GATGTGACTA ATACTGAATT GAGGCTTTTA 
TTCTCCAGCT CTGCAGCATT TGCAAAATTT GAGATAACTT TGCCTCTCTC AGTCCATTTT 
CCAACTGTTC CATTACCTTT CTCCATTCAA AAGCACCTTG GAAACATTGG CCAAGATGAA 
ATCACTGCAA TTCTATCTAA AGTGCCACTG AAGAACAACT ATCTAAAGAA TGTAGTCAAG 
CAAATTTACC AAGATCTGCT TCAGGACTGC CATTTCTATC ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KNL1 ( XM_005638256.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005638313.1 Canis lupus familiaris cancer susceptibility candidate 5 (CASC5), transcript variant X4, mRNA.

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CloneID	OCe28662
Clone ID Related Accession (Same CDS sequence)	XM_005638256.2 , XM_005638256.1
Accession Version	XM_005638256.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6825bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	protein CASC5 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726113.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGGGA TGTCTTCAGA GACTAATGAA GAGAATGACA ATATGGAGAG ACCTGTTAGA 
AGACGACATT CCTCCATTTT GAAACCCCCA AGGAGTCCTC TTCAGGACCT CAGAGGTGGG 
AATGAAGCAG TTCAGGAATC TAATGCTTTG AGAAATAAGA AAAGCTCTCG TCGAGTCAGC 
TTTGCAGATA CCATAAAAGT ATTCCAAACA GAGTCTCATA TGAAAATAGT AAGAAAGTCA 
GAAATTGCAG AAACAGAAGC AGGAGAAAAT GTTCTATATA TACAGAATAA GAAGCCAGAA 
GAAACCTACT GTGAAATTAC TGGGATGAAC ACATTGCTTT CTGCTCCTAT TCAGACTCAG 
ATGCAACAGA GAGAGATTTC AATTACAGAA CACAACCATG AAATGAAACC TGCAAATGAC 
CAAACAGTCA TCTTTTCAGA TGAAAACCAG ATGGACCTAA CAGCAAGTCA CACCGTAATG 
ATTACCAAAG ACCTGTTAGA TTGTACTAAA AGTGAAAAGT CCATTAAGAT AGATACCGCA 
TCATTTCTGG CTAACTTAAA GCTCCATACT AAGGATTCAA GAATGAAAAA AGAATTAAAT 
TTTTCCATGG ATCAAAGCAC TTCTTCAGAA AAGAAAATAC ATTTTAATGA CTTCATAAAA 
AGATTGAAAA CAGGAAAATC TAATGCTTTT CCTACAGGAC CTGATAAAGA AAATTTTGAA 
ATATCTGTTC ATCCCATAAA AACAAAGAAG GCCTCTTCTG TACATCAAAT GCATGTATCT 
CTTAGGGTAG ATGAAAATAG TAGTAATACA ACTAGAATCT TCGGAGAACA AAATGATGGG 
ATGAATTTCA CTCAGTGTCA TACAGCCAGT ATTCAGACTT TGGTTCCCAC ATCCAGTAAG 
GCCAGCATAG GGGAATTTAA AGGTGATGAT ATTACAATTT ATGGCAGTGA TTTTATGGAC 
TTGACAACTA ACCACACTAT ACAGAATTTA CCCTCAGCAG ATAATTCATC TAATAAAGAA 
AATCAAACTC AGAATGTCAT GATGGAGGTT AAAGCTCCAG GAGGGAAGAC AATCTTTAAT 
AAACTGAATA TTCCTTTTCA AACCCCTTTA AACCCTAATG GTGAAATACA TATTACCAAA 
AGTCATATTA CAGGGACAGA AACTCACACA AGCACACAGA CTTCTAACCA AGATGCCAGA 
ACATTGACTA TAATCCCAGA ATCTATATGT TCCAGTCCAG CTACTCAAGA TTATAAGACA 
TTTTTCTATT CTAGCAGTAA TAATGCCATG GAACTGACCA CGTGTCTCTC GGGTATGAGA 
AAAGAGAAGA ATTTGCTGAA GAATGATAAT TATTCTAAAA TGTATCCCAA TCCAGATGCC 
ATCTCTCTTC TTACAGAGAA AACTATTTAT TCAGGAGAGG ATAGCATGGA CATTACGAGG 
AGTCATACAG TTGCAATAGA TAATCAGATT TTTAAACAAG ATCAGACAAA TATACAGAAA 
GCAGACACAT CAATATCTGA AAAAGAAATA ATGCTCCAAA ATCTCATAAC CATGTCAAAG 
GACGGGAAAA TGAATATAAA TTGTAGCTCA GTTCCTCATA TATCTAAGGG AAGATTACAG 
CAGAGCCTGG CAAACTCTTT ATCTATTTCA TTGACTGACA AAAAGACTGA AATCTTCACA 
GGTGAAGATA TGGATTTGAC TAAAAGTCAC ACAACTAACT TAGGAAGTCA AGCTCCACTT 
ACATCTTACA ATGTAACATC TGAGAATACC AGTAAATTTC TCTCTCACAG CAAAAGCCCT 
TCAGGTGAAT GGCAAGAAAT GACCAAAAGT CGTATTGAAC CATCCCAGCA GCCAGACATA 
ATATCTAAAA ACATCCCAGT GGACACCTGG GGCAAAGAAA AAGTTCAAGT TTTAGAGATT 
GCACCTTATG TTGATAAAGA TTCTCCTCAA TCAGCTGATA TTAATCAAGA CATTGCAATA 
AGCCATAGTA TAGTATACTC TGATAGAAAT CTTGAGAAAC AAGTAGCACT AGGAAATAAT 
AGAAATACTG TTCCATGTGA GCAATCTTTG TTTCCTACTT CAGAGTCATT ATTTTCATTA 
GAAGGACAGT CTAACATGAA AAATCATAAT ATTGCTATTA ACAGTTACAC AATGAAATCT 
GTGCCAGGCC AGAATTCTAA ACTGCCTGAG CCACTGAGGA AAGGTGTAGG CAATCCAACA 
CCTGACTATT CTCATGACAA AACAATTATT TGTTCAGAGG AGGAACAAAA TATGGATCTA 
ACCAAGAGTC ACACTGTTGT CATTGGATTT AGTCCTTCTG AAATACAAGA ATTGGGTAAG 
ACTAATCTAG AAAATACTAA CAGTCTGCTA ACAACAGCAA ACAGACAGAC AGCTATAAAA 
GTTGAAAAAT GTTGTAAAAG TCCTATAGAA AAAACAGGAA TATCTGTTTC TAAGGATAAC 
CTAGATGTGA TAGAGGACAA TAGCATACAG AAACTTGGAT TTTTGAATGA AAAGCAAGAT 
GTCAAAATTT GTGGAAGAAA AAGTGTTGGC AGACAAAAAG TTGATAAGAC TATTGTATTT 
TCAGAGGGTG ATGAGAATGA TATGGATATC ACCAAGATTT TTACAGTGGA AATAAATCAT 
AAACCTTTAT TACTTGAACA AGATTCCCAT TTGGCAGGAA CTTCTAAAAC TGTTTTGTAT 
GAATGTGGGC AAGATGATGT GGAGATCACT AAAAGTCACA CAACTGCCTT GGAATGTATA 
ACTGTCTCAC CAGATAAAAT AACCAGTAGG CCTATGGACA AAACTGTAAT GTTTGTAGAT 
AATTACAATG AACTGGAAAT GACAAAGTCT CACACTGTTT TCATTGACTA CCAAGCAAAG 
GAGAGGACTG TGAGTATAGA CAAACTTGAC ACGGAACTAT CCAAAAGAAG AAAAAGCATA 
GAAAAGCCAA AAATGACACC TGCTTCTGCT GAGGAGAGTG TTTCCTTTCT GGAGAATGGT 
GAAAGTGACC ATTCAGCAGC AAACGTCAAT AAGCTAACAC TACTGAGGGA ATGGTCTAAT 
AATGGTCCTA TAGAGAAAGC AGTAGCTGGT GATAACATGG AAGTATCTAA ATTCACCATT 
TGGAAAAATG GCAAAGATGT ACAAAAGCCT GAATTTCTGA ATGACACTAT ATCAGACAAA 
AGTCAGAGAA GGAAAAGCCT TAGACTCAAA AATGACAAGA TTGCTGCATT TTCTAAAAAT 
GATGATGATA TGGATATCAC CCAGAGTTGT ACAGTGAAAA TAAACTATAA AAGTGCCCCA 
GAAGATAGAG AGGATTCCCA TTTGGTGCCT TTGACAGGAG CTTCTAAAAC TGTTTTGTAT 
TCATGTGGGC AGGATGACAT GGAGATGACT AGAAGTCATA CGACTGCGTT AGAATGTAAA 
ACTGTCTCAC CGGATAAAAT CACTACTAGG CCCATAGATA AAACTGTAAT GTTTGTTGAT 
AATCATGGTG ATCTGGAAGT CACCGAGTCC CATACTGTTT TCATTGATTG TCAAAACACA 
GAGAAAATTA ATCAAGAGTG CCCTAAATTT GGAATAGCTA CAGGAAAACC CTTGGGTATT 
TCTTTTCATA ATGATGGTAG CTCTGTTCAA GAAATCACTG AAAAACAAGC ATTGGCAGTA 
GACCAGACCT ATATACCGGA AAAAGCCAGA ATTGGAAGCT GTCAGTTAAA TAGTACAGAT 
AGAAGAAACA TGGACTTTAC TAACAGTCAA GCAACTGCTA CGTGTGGATC CAGTGATAAT 
TATTCCTGTT TACCAAATGC TATTTCCTGT TTTGATAATT TAGAGGGGAA TGTCATGTCC 
TTAAGTAATA AAGATAAGAA AGCCAGTAAC TGCCCAGTGC AAAATGATCT TTCTTATGTA 
AAGGATTTAG CCAATGAATA TTACTTGAAA CCTGAGGGAC TGCCTCCCTC TGCTTGTTCT 
TTGTTAAAGA AAGAAAAAGT GACTCAAACC AATACCAATG GACAGTTAGA CTGTGTTACA 
ACAGTGCTTA AAGATCAAGA TATGATTAAG GAGTCCCAGA ATCTAATAGC TAATCAAACT 
TTACAAGATA GTCAGGAGCT GGCGGAGATG ATTAAACCTA ATTCAAATCG AGTATCCTTT 
AAACCCCCAA AGGATCAAAT GGAGGGCTTT GTTGATACCA CGGAACATAA TTTAAAGAAG 
ACTGTTGTTG ATAATGTTTG TGTTACTTCT CAGCCTCATC TCACAAGCCA GGTACCTCCA 
TTACCTCAAC AAGGACGGAT AATTTTGAAT AAAGATGAAA CAATATCATC TAATTCTGGA 
AATAAGAATT TAAATATTGT TATAGGAAAT TCCTTTGTAC CCACATGTGA AAACGAGGCC 
AAAATGCTCA ACAATGATAA ACAATTTACC ATAGTCTGTA AAAATGAGCT GAGGAAAAAT 
ACTAAATGTA ATACCGATTT CCACAGTAAT TCAGACTTGA CTAAGCAAGT TATTCAAACT 
CATACTAATT CTAGAGAAGC ATCAGATACT GTAATTGCAT CTAATACAAG TTTTAATAGT 
GTCAGATCAA ATCTGAATAA TTTGAATGGA AAAACTGAAG AATTTTTAGA TTTCCAAACT 
CCCCATATTC CATCTTCTCC AGAGCAAATA CTTGCATTAA TAAACAAGGC ACACAATCAT 
ATGAGTATAT TGCAAGCTAC AGAAATACAG AACATTAACC CAGCGCCCAG CAATGTTGAA 
GATGATAAAG ATGAAGAAAG CAAAATTTCT CATAATGGAG CTGAAACCAA CTCTGTACCA 
CTCATGACAG TTGTAAAAGA TAAAGTGAAG AGATGTTCTT TGGGAATATT TTTGCCCAGA 
TTGCCAAACA AGAGAAATTG TAGTGTCACT GGTATTAATG ACCTGAAGCA GATTCAAGGA 
GACACAACTG ATTTAAATCA CTTAGAAATT CAGCCAGTCT TCAGTAAGGA GCCAAGCATT 
GAATTTGTTG CAACTAAACT GACCTTAAGT CCATCTCAGT ATATAAATGA GGAAAACTTT 
CCTATATATC CTGGTGAGAT TAATTCCTCA GATTCTATTA CCATAGAAAC TGAGGAAAAG 
GCTTTGATTG AGACATATCA AAAAGGGATT CCACCATCTG AAAATAAGAT AAGAGAAACC 
TGCAGTAACC AAAAAAGAAC CTGGGTACAA GAAGATGATG ATATTCAGAA TGAGAAAAAA 
ATCAGGAAAA GTGAGATTAG GTGTAGTGAT ACTACACAAG ATCAGGAGAT TTTTGATCAC 
CATGCTGAAG GGGATATAGA TAAAAATGCT AACAGCCTGA GCAGAACCCC ATCTAGTTGC 
AGCAGTTCTC TGGATTCGAT AAAGGCTGAT GGGACCTCTC TGGACTTCAG CACACAGCAC 
AATAGTCAAA TGGAATCACA GTTTCTCAGA GACACTATTT GTGAAGAGAG CTTGAAGGAG 
AAACTCAAAG ATGGGAAAAT TACAATAAAG GAGTTCTTTA TACTTCTTCA GGTCCACATT 
TTGATACAGA AACCCCGACA GAGCAATCTC CCAGCCAAAT TTACTATAAA CACACCACCT 
ACTCTGGAAG ACCTGATGTT AAGTCAGTAT GTTTACCGAC CCAAGATACA GATTTATAGA 
GAAGATTGTG AAGCTCTTTG CCAAAAGATT GAAGAATTAA AGCTTTCTGC ATTGAACCAG 
GATAAATTGT TGACTGACAT AAATAGGAAC CTGTGGGAAA AAATGAGACA CTGCTCTGAT 
GAAGAGCTGA AGGTCTTTGG AATTTACCTG AACAAAATAA AATCACATTT TACCAAGATG 
ACTAAAGTTT TTACTCACCA AGGAAAAGTG GCTCTCTATA CCAAGCTGGT ACAGTCAGCT 
CAGAATGAGA GGAAGAAACT TCAGATAAGG ATAGATGAGA TGGACAACAT ACTTAAGAAG 
ATTGAGAACT GTCTCGCTGA AGTTGAAATA GAAACTAAGA ATTTGGATGA AGAGAAAGAC 
AATCCTATGG AAGAATGGGA TTCTGAAATG AGAGCCGCAG AGAAAGAACT AGAACAGCTG 
AAAACTGAAG AAGATGAGCT CCAAAGAAAT CTCTTAGAAC TGGAAGTACA GAAAGAGCAG 
ACCCTTGCTC AAATAGACTT TGTACAAAAA CAAACAGATA GAACTGAAGA GCTAATGGAT 
CAGTTGAGCT TGTCTGAGTG GGAAGCCATT GAGTGGAGTG ATGATCAAGC TGTATTCACC 
TTTCTTTATG ACACAATAGA ACTTACTATC ACCTTTGGAG AGACAGTAGT TGATCTCCCT 
TTCCTGGACA AGGCTTGTAG GAAGATTGTT GACTTGAATT TTCAATCTCG GTTAGATGAG 
GATAAAGCTC CTCCTTCCTC CCTTTTAGTT CATAAGCTTA TTTTCCAGTA CATTGAGGAA 
AAGGAATCCT GGAAGAAAAA ATGTACAACA CAGCATCAAA TACCCAAGAT GCTTCAAGAA 
ATCTCATTGG TAGTGAGCCA TTGTAGACTC CTTGGAGAGG AAATTGAGTT TTTAAAGAGA 
TGGGGACCAA ATTATAACCT AATGAACATA GATGTGACTA ATACTGAATT GAGGCTTTTA 
TTCTCCAGCT CTGCAGCATT TGCAAAATTT GAGATAACTT TGCCTCTCTC AGTCCATTTT 
CCAACTGTTC CATTACCTTT CTCCATTCAA AAGCACCTTG GAAACATTGG CCAAGATGAA 
ATCACTGCAA TTCTATCTAA AGTGCCACTG AAGAACAACT ATCTAAAGAA TGTAGTCAAG 
CAAATTTACC AAGATCTGCT TCAGGACTGC CATTTCTATC ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005638313.1
CDS	354..7178
Translation	MDGMSSETNEENDNMERPVRRRHSSILKPPRSPLQDLRGGNEAVQESNALRNKKSSRRVSFADTIKVFQTESHMKIVRKSEIAETEAGENVLYIQNKKPEETYCEITGMNTLLSAPIQTQMQQREISITEHNHEMKPANDQTVIFSDENQMDLTASHTVMITKDLLDCTKSEKSIKIDTASFLANLKLHTKDSRMKKELNFSMDQSTSSEKKIHFNDFIKRLKTGKSNAFPTGPDKENFEISVHPIKTKKASSVHQMHVSLRVDENSSNTTRIFGEQNDGMNFTQCHTASIQTLVPTSSKASIGEFKGDDITIYGSDFMDLTTNHTIQNLPSADNSSNKENQTQNVMMEVKAPGGKTIFNKLNIPFQTPLNPNGEIHITKSHITGTETHTSTQTSNQDARTLTIIPESICSSPATQDYKTFFYSSSNNAMELTTCLSGMRKEKNLLKNDNYSKMYPNPDAISLLTEKTIYSGEDSMDITRSHTVAIDNQIFKQDQTNIQKADTSISEKEIMLQNLITMSKDGKMNINCSSVPHISKGRLQQSLANSLSISLTDKKTEIFTGEDMDLTKSHTTNLGSQAPLTSYNVTSENTSKFLSHSKSPSGEWQEMTKSRIEPSQQPDIISKNIPVDTWGKEKVQVLEIAPYVDKDSPQSADINQDIAISHSIVYSDRNLEKQVALGNNRNTVPCEQSLFPTSESLFSLEGQSNMKNHNIAINSYTMKSVPGQNSKLPEPLRKGVGNPTPDYSHDKTIICSEEEQNMDLTKSHTVVIGFSPSEIQELGKTNLENTNSLLTTANRQTAIKVEKCCKSPIEKTGISVSKDNLDVIEDNSIQKLGFLNEKQDVKICGRKSVGRQKVDKTIVFSEGDENDMDITKIFTVEINHKPLLLEQDSHLAGTSKTVLYECGQDDVEITKSHTTALECITVSPDKITSRPMDKTVMFVDNYNELEMTKSHTVFIDYQAKERTVSIDKLDTELSKRRKSIEKPKMTPASAEESVSFLENGESDHSAANVNKLTLLREWSNNGPIEKAVAGDNMEVSKFTIWKNGKDVQKPEFLNDTISDKSQRRKSLRLKNDKIAAFSKNDDDMDITQSCTVKINYKSAPEDREDSHLVPLTGASKTVLYSCGQDDMEMTRSHTTALECKTVSPDKITTRPIDKTVMFVDNHGDLEVTESHTVFIDCQNTEKINQECPKFGIATGKPLGISFHNDGSSVQEITEKQALAVDQTYIPEKARIGSCQLNSTDRRNMDFTNSQATATCGSSDNYSCLPNAISCFDNLEGNVMSLSNKDKKASNCPVQNDLSYVKDLANEYYLKPEGLPPSACSLLKKEKVTQTNTNGQLDCVTTVLKDQDMIKESQNLIANQTLQDSQELAEMIKPNSNRVSFKPPKDQMEGFVDTTEHNLKKTVVDNVCVTSQPHLTSQVPPLPQQGRIILNKDETISSNSGNKNLNIVIGNSFVPTCENEAKMLNNDKQFTIVCKNELRKNTKCNTDFHSNSDLTKQVIQTHTNSREASDTVIASNTSFNSVRSNLNNLNGKTEEFLDFQTPHIPSSPEQILALINKAHNHMSILQATEIQNINPAPSNVEDDKDEESKISHNGAETNSVPLMTVVKDKVKRCSLGIFLPRLPNKRNCSVTGINDLKQIQGDTTDLNHLEIQPVFSKEPSIEFVATKLTLSPSQYINEENFPIYPGEINSSDSITIETEEKALIETYQKGIPPSENKIRETCSNQKRTWVQEDDDIQNEKKIRKSEIRCSDTTQDQEIFDHHAEGDIDKNANSLSRTPSSCSSSLDSIKADGTSLDFSTQHNSQMESQFLRDTICEESLKEKLKDGKITIKEFFILLQVHILIQKPRQSNLPAKFTINTPPTLEDLMLSQYVYRPKIQIYREDCEALCQKIEELKLSALNQDKLLTDINRNLWEKMRHCSDEELKVFGIYLNKIKSHFTKMTKVFTHQGKVALYTKLVQSAQNERKKLQIRIDEMDNILKKIENCLAEVEIETKNLDEEKDNPMEEWDSEMRAAEKELEQLKTEEDELQRNLLELEVQKEQTLAQIDFVQKQTDRTEELMDQLSLSEWEAIEWSDDQAVFTFLYDTIELTITFGETVVDLPFLDKACRKIVDLNFQSRLDEDKAPPSSLLVHKLIFQYIEEKESWKKKCTTQHQIPKMLQEISLVVSHCRLLGEEIEFLKRWGPNYNLMNIDVTNTELRLLFSSSAAFAKFEITLPLSVHFPTVPLPFSIQKHLGNIGQDEITAILSKVPLKNNYLKNVVKQIYQDLLQDCHFYH

Target ORF information:

RefSeq Version	XM_005638256.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris cancer susceptibility candidate 5 (CASC5), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005638256.1

ORF Insert Sequence:

ATGGATGGGA TGTCTTCAGA GACTAATGAA GAGAATGACA ATATGGAGAG ACCTGTTAGA 
AGACGACATT CCTCCATTTT GAAACCCCCA AGGAGTCCTC TTCAGGACCT CAGAGGTGGG 
AATGAAGCAG TTCAGGAATC TAATGCTTTG AGAAATAAGA AAAGCTCTCG TCGAGTCAGC 
TTTGCAGATA CCATAAAAGT ATTCCAAACA GAGTCTCATA TGAAAATAGT AAGAAAGTCA 
GAAATTGCAG AAACAGAAGC AGGAGAAAAT GTTCTATATA TACAGAATAA GAAGCCAGAA 
GAAACCTACT GTGAAATTAC TGGGATGAAC ACATTGCTTT CTGCTCCTAT TCAGACTCAG 
ATGCAACAGA GAGAGATTTC AATTACAGAA CACAACCATG AAATGAAACC TGCAAATGAC 
CAAACAGTCA TCTTTTCAGA TGAAAACCAG ATGGACCTAA CAGCAAGTCA CACCGTAATG 
ATTACCAAAG ACCTGTTAGA TTGTACTAAA AGTGAAAAGT CCATTAAGAT AGATACCGCA 
TCATTTCTGG CTAACTTAAA GCTCCATACT AAGGATTCAA GAATGAAAAA AGAATTAAAT 
TTTTCCATGG ATCAAAGCAC TTCTTCAGAA AAGAAAATAC ATTTTAATGA CTTCATAAAA 
AGATTGAAAA CAGGAAAATC TAATGCTTTT CCTACAGGAC CTGATAAAGA AAATTTTGAA 
ATATCTGTTC ATCCCATAAA AACAAAGAAG GCCTCTTCTG TACATCAAAT GCATGTATCT 
CTTAGGGTAG ATGAAAATAG TAGTAATACA ACTAGAATCT TCGGAGAACA AAATGATGGG 
ATGAATTTCA CTCAGTGTCA TACAGCCAGT ATTCAGACTT TGGTTCCCAC ATCCAGTAAG 
GCCAGCATAG GGGAATTTAA AGGTGATGAT ATTACAATTT ATGGCAGTGA TTTTATGGAC 
TTGACAACTA ACCACACTAT ACAGAATTTA CCCTCAGCAG ATAATTCATC TAATAAAGAA 
AATCAAACTC AGAATGTCAT GATGGAGGTT AAAGCTCCAG GAGGGAAGAC AATCTTTAAT 
AAACTGAATA TTCCTTTTCA AACCCCTTTA AACCCTAATG GTGAAATACA TATTACCAAA 
AGTCATATTA CAGGGACAGA AACTCACACA AGCACACAGA CTTCTAACCA AGATGCCAGA 
ACATTGACTA TAATCCCAGA ATCTATATGT TCCAGTCCAG CTACTCAAGA TTATAAGACA 
TTTTTCTATT CTAGCAGTAA TAATGCCATG GAACTGACCA CGTGTCTCTC GGGTATGAGA 
AAAGAGAAGA ATTTGCTGAA GAATGATAAT TATTCTAAAA TGTATCCCAA TCCAGATGCC 
ATCTCTCTTC TTACAGAGAA AACTATTTAT TCAGGAGAGG ATAGCATGGA CATTACGAGG 
AGTCATACAG TTGCAATAGA TAATCAGATT TTTAAACAAG ATCAGACAAA TATACAGAAA 
GCAGACACAT CAATATCTGA AAAAGAAATA ATGCTCCAAA ATCTCATAAC CATGTCAAAG 
GACGGGAAAA TGAATATAAA TTGTAGCTCA GTTCCTCATA TATCTAAGGG AAGATTACAG 
CAGAGCCTGG CAAACTCTTT ATCTATTTCA TTGACTGACA AAAAGACTGA AATCTTCACA 
GGTGAAGATA TGGATTTGAC TAAAAGTCAC ACAACTAACT TAGGAAGTCA AGCTCCACTT 
ACATCTTACA ATGTAACATC TGAGAATACC AGTAAATTTC TCTCTCACAG CAAAAGCCCT 
TCAGGTGAAT GGCAAGAAAT GACCAAAAGT CGTATTGAAC CATCCCAGCA GCCAGACATA 
ATATCTAAAA ACATCCCAGT GGACACCTGG GGCAAAGAAA AAGTTCAAGT TTTAGAGATT 
GCACCTTATG TTGATAAAGA TTCTCCTCAA TCAGCTGATA TTAATCAAGA CATTGCAATA 
AGCCATAGTA TAGTATACTC TGATAGAAAT CTTGAGAAAC AAGTAGCACT AGGAAATAAT 
AGAAATACTG TTCCATGTGA GCAATCTTTG TTTCCTACTT CAGAGTCATT ATTTTCATTA 
GAAGGACAGT CTAACATGAA AAATCATAAT ATTGCTATTA ACAGTTACAC AATGAAATCT 
GTGCCAGGCC AGAATTCTAA ACTGCCTGAG CCACTGAGGA AAGGTGTAGG CAATCCAACA 
CCTGACTATT CTCATGACAA AACAATTATT TGTTCAGAGG AGGAACAAAA TATGGATCTA 
ACCAAGAGTC ACACTGTTGT CATTGGATTT AGTCCTTCTG AAATACAAGA ATTGGGTAAG 
ACTAATCTAG AAAATACTAA CAGTCTGCTA ACAACAGCAA ACAGACAGAC AGCTATAAAA 
GTTGAAAAAT GTTGTAAAAG TCCTATAGAA AAAACAGGAA TATCTGTTTC TAAGGATAAC 
CTAGATGTGA TAGAGGACAA TAGCATACAG AAACTTGGAT TTTTGAATGA AAAGCAAGAT 
GTCAAAATTT GTGGAAGAAA AAGTGTTGGC AGACAAAAAG TTGATAAGAC TATTGTATTT 
TCAGAGGGTG ATGAGAATGA TATGGATATC ACCAAGATTT TTACAGTGGA AATAAATCAT 
AAACCTTTAT TACTTGAACA AGATTCCCAT TTGGCAGGAA CTTCTAAAAC TGTTTTGTAT 
GAATGTGGGC AAGATGATGT GGAGATCACT AAAAGTCACA CAACTGCCTT GGAATGTATA 
ACTGTCTCAC CAGATAAAAT AACCAGTAGG CCTATGGACA AAACTGTAAT GTTTGTAGAT 
AATTACAATG AACTGGAAAT GACAAAGTCT CACACTGTTT TCATTGACTA CCAAGCAAAG 
GAGAGGACTG TGAGTATAGA CAAACTTGAC ACGGAACTAT CCAAAAGAAG AAAAAGCATA 
GAAAAGCCAA AAATGACACC TGCTTCTGCT GAGGAGAGTG TTTCCTTTCT GGAGAATGGT 
GAAAGTGACC ATTCAGCAGC AAACGTCAAT AAGCTAACAC TACTGAGGGA ATGGTCTAAT 
AATGGTCCTA TAGAGAAAGC AGTAGCTGGT GATAACATGG AAGTATCTAA ATTCACCATT 
TGGAAAAATG GCAAAGATGT ACAAAAGCCT GAATTTCTGA ATGACACTAT ATCAGACAAA 
AGTCAGAGAA GGAAAAGCCT TAGACTCAAA AATGACAAGA TTGCTGCATT TTCTAAAAAT 
GATGATGATA TGGATATCAC CCAGAGTTGT ACAGTGAAAA TAAACTATAA AAGTGCCCCA 
GAAGATAGAG AGGATTCCCA TTTGGTGCCT TTGACAGGAG CTTCTAAAAC TGTTTTGTAT 
TCATGTGGGC AGGATGACAT GGAGATGACT AGAAGTCATA CGACTGCGTT AGAATGTAAA 
ACTGTCTCAC CGGATAAAAT CACTACTAGG CCCATAGATA AAACTGTAAT GTTTGTTGAT 
AATCATGGTG ATCTGGAAGT CACCGAGTCC CATACTGTTT TCATTGATTG TCAAAACACA 
GAGAAAATTA ATCAAGAGTG CCCTAAATTT GGAATAGCTA CAGGAAAACC CTTGGGTATT 
TCTTTTCATA ATGATGGTAG CTCTGTTCAA GAAATCACTG AAAAACAAGC ATTGGCAGTA 
GACCAGACCT ATATACCGGA AAAAGCCAGA ATTGGAAGCT GTCAGTTAAA TAGTACAGAT 
AGAAGAAACA TGGACTTTAC TAACAGTCAA GCAACTGCTA CGTGTGGATC CAGTGATAAT 
TATTCCTGTT TACCAAATGC TATTTCCTGT TTTGATAATT TAGAGGGGAA TGTCATGTCC 
TTAAGTAATA AAGATAAGAA AGCCAGTAAC TGCCCAGTGC AAAATGATCT TTCTTATGTA 
AAGGATTTAG CCAATGAATA TTACTTGAAA CCTGAGGGAC TGCCTCCCTC TGCTTGTTCT 
TTGTTAAAGA AAGAAAAAGT GACTCAAACC AATACCAATG GACAGTTAGA CTGTGTTACA 
ACAGTGCTTA AAGATCAAGA TATGATTAAG GAGTCCCAGA ATCTAATAGC TAATCAAACT 
TTACAAGATA GTCAGGAGCT GGCGGAGATG ATTAAACCTA ATTCAAATCG AGTATCCTTT 
AAACCCCCAA AGGATCAAAT GGAGGGCTTT GTTGATACCA CGGAACATAA TTTAAAGAAG 
ACTGTTGTTG ATAATGTTTG TGTTACTTCT CAGCCTCATC TCACAAGCCA GGTACCTCCA 
TTACCTCAAC AAGGACGGAT AATTTTGAAT AAAGATGAAA CAATATCATC TAATTCTGGA 
AATAAGAATT TAAATATTGT TATAGGAAAT TCCTTTGTAC CCACATGTGA AAACGAGGCC 
AAAATGCTCA ACAATGATAA ACAATTTACC ATAGTCTGTA AAAATGAGCT GAGGAAAAAT 
ACTAAATGTA ATACCGATTT CCACAGTAAT TCAGACTTGA CTAAGCAAGT TATTCAAACT 
CATACTAATT CTAGAGAAGC ATCAGATACT GTAATTGCAT CTAATACAAG TTTTAATAGT 
GTCAGATCAA ATCTGAATAA TTTGAATGGA AAAACTGAAG AATTTTTAGA TTTCCAAACT 
CCCCATATTC CATCTTCTCC AGAGCAAATA CTTGCATTAA TAAACAAGGC ACACAATCAT 
ATGAGTATAT TGCAAGCTAC AGAAATACAG AACATTAACC CAGCGCCCAG CAATGTTGAA 
GATGATAAAG ATGAAGAAAG CAAAATTTCT CATAATGGAG CTGAAACCAA CTCTGTACCA 
CTCATGACAG TTGTAAAAGA TAAAGTGAAG AGATGTTCTT TGGGAATATT TTTGCCCAGA 
TTGCCAAACA AGAGAAATTG TAGTGTCACT GGTATTAATG ACCTGAAGCA GATTCAAGGA 
GACACAACTG ATTTAAATCA CTTAGAAATT CAGCCAGTCT TCAGTAAGGA GCCAAGCATT 
GAATTTGTTG CAACTAAACT GACCTTAAGT CCATCTCAGT ATATAAATGA GGAAAACTTT 
CCTATATATC CTGGTGAGAT TAATTCCTCA GATTCTATTA CCATAGAAAC TGAGGAAAAG 
GCTTTGATTG AGACATATCA AAAAGGGATT CCACCATCTG AAAATAAGAT AAGAGAAACC 
TGCAGTAACC AAAAAAGAAC CTGGGTACAA GAAGATGATG ATATTCAGAA TGAGAAAAAA 
ATCAGGAAAA GTGAGATTAG GTGTAGTGAT ACTACACAAG ATCAGGAGAT TTTTGATCAC 
CATGCTGAAG GGGATATAGA TAAAAATGCT AACAGCCTGA GCAGAACCCC ATCTAGTTGC 
AGCAGTTCTC TGGATTCGAT AAAGGCTGAT GGGACCTCTC TGGACTTCAG CACACAGCAC 
AATAGTCAAA TGGAATCACA GTTTCTCAGA GACACTATTT GTGAAGAGAG CTTGAAGGAG 
AAACTCAAAG ATGGGAAAAT TACAATAAAG GAGTTCTTTA TACTTCTTCA GGTCCACATT 
TTGATACAGA AACCCCGACA GAGCAATCTC CCAGCCAAAT TTACTATAAA CACACCACCT 
ACTCTGGAAG ACCTGATGTT AAGTCAGTAT GTTTACCGAC CCAAGATACA GATTTATAGA 
GAAGATTGTG AAGCTCTTTG CCAAAAGATT GAAGAATTAA AGCTTTCTGC ATTGAACCAG 
GATAAATTGT TGACTGACAT AAATAGGAAC CTGTGGGAAA AAATGAGACA CTGCTCTGAT 
GAAGAGCTGA AGGTCTTTGG AATTTACCTG AACAAAATAA AATCACATTT TACCAAGATG 
ACTAAAGTTT TTACTCACCA AGGAAAAGTG GCTCTCTATA CCAAGCTGGT ACAGTCAGCT 
CAGAATGAGA GGAAGAAACT TCAGATAAGG ATAGATGAGA TGGACAACAT ACTTAAGAAG 
ATTGAGAACT GTCTCGCTGA AGTTGAAATA GAAACTAAGA ATTTGGATGA AGAGAAAGAC 
AATCCTATGG AAGAATGGGA TTCTGAAATG AGAGCCGCAG AGAAAGAACT AGAACAGCTG 
AAAACTGAAG AAGATGAGCT CCAAAGAAAT CTCTTAGAAC TGGAAGTACA GAAAGAGCAG 
ACCCTTGCTC AAATAGACTT TGTACAAAAA CAAACAGATA GAACTGAAGA GCTAATGGAT 
CAGTTGAGCT TGTCTGAGTG GGAAGCCATT GAGTGGAGTG ATGATCAAGC TGTATTCACC 
TTTCTTTATG ACACAATAGA ACTTACTATC ACCTTTGGAG AGACAGTAGT TGATCTCCCT 
TTCCTGGACA AGGCTTGTAG GAAGATTGTT GACTTGAATT TTCAATCTCG GTTAGATGAG 
GATAAAGCTC CTCCTTCCTC CCTTTTAGTT CATAAGCTTA TTTTCCAGTA CATTGAGGAA 
AAGGAATCCT GGAAGAAAAA ATGTACAACA CAGCATCAAA TACCCAAGAT GCTTCAAGAA 
ATCTCATTGG TAGTGAGCCA TTGTAGACTC CTTGGAGAGG AAATTGAGTT TTTAAAGAGA 
TGGGGACCAA ATTATAACCT AATGAACATA GATGTGACTA ATACTGAATT GAGGCTTTTA 
TTCTCCAGCT CTGCAGCATT TGCAAAATTT GAGATAACTT TGCCTCTCTC AGTCCATTTT 
CCAACTGTTC CATTACCTTT CTCCATTCAA AAGCACCTTG GAAACATTGG CCAAGATGAA 
ATCACTGCAA TTCTATCTAA AGTGCCACTG AAGAACAACT ATCTAAAGAA TGTAGTCAAG 
CAAATTTACC AAGATCTGCT TCAGGACTGC CATTTCTATC ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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KNL1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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