Rsph4a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rsph4a
Entrez Gene ID	309767
Full Name	radial spoke head 4 homolog A
Synonyms	Rshl3
General protein information	Preferred Names radial spoke head 4 homolog A Names radial spoke head protein 4 homolog A radial spokehead-like 3
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 20 Map Location: 20q11

mRNA and Protein(s)

mRNA	Protein	Name
NM_001107629.2	NP_001101099.2	radial spoke head protein 4 homolog A
XM_017588068.1	XP_017443557.1	radial spoke head protein 4 homolog A
XM_003751961.4	XP_003752009.1	radial spoke head protein 4 homolog A

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	RSPH4A	XP_541210.2
Mus musculus (house mouse)	Rsph4a	NP_001156429.1
Gallus gallus (chicken)	RSPH4A	XP_420671.4
Bos taurus (cattle)	RSPH4A	XP_002690133.1
Pan troglodytes (chimpanzee)	RSPH4A	XP_001162376.1
Xenopus tropicalis (tropical clawed frog)	rsph6a	NP_001016760.1
Danio rerio (zebrafish)	LOC100148286	XP_001923770.1
Homo sapiens (human)	RSPH4A	NP_001010892.1
Macaca mulatta (Rhesus monkey)	RSPH4A	XP_001111183.1
Rattus norvegicus (Norway rat)	Rsph4a	XP_003752013.1

Gene Ontology
Bibliography

Related articles in PubMed

Mutations in CCDC39 and CCDC40 are the major cause of primary ciliary dyskinesia with axonemal disorganization and absent inner dynein arms.
Antony D, Becker-Heck A, Zariwala MA, Schmidts M, Onoufriadis A, Forouhan M, Wilson R, Taylor-Cox T, Dewar A, Jackson C, Goggin P, Loges NT, Olbrich H, Jaspers M, Jorissen M, Leigh MW, Wolf WE, Daniels ML, Noone PG, Ferkol TW, Sagel SD, Rosenfeld M, Rutman A, Dixit A, O'Callaghan C, Lucas JS, Hogg C, Scambler PJ, Emes RD, , Chung EM, Shoemark A, Knowles MR, Omran H, Mitchison HM
Human mutation34(3)462-72(2013 Mar)

Mutations in radial spoke head protein genes RSPH9 and RSPH4A cause primary ciliary dyskinesia with central-microtubular-pair abnormalities.
Castleman VH, Romio L, Chodhari R, Hirst RA, de Castro SC, Parker KA, Ybot-Gonzalez P, Emes RD, Wilson SW, Wallis C, Johnson CA, Herrera RJ, Rutman A, Dixon M, Shoemark A, Bush A, Hogg C, Gardiner RM, Reish O, Greene ND, O'Callaghan C, Purton S, Chung EM, Mitchison HM
American journal of human genetics84(2)197-209(2009 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Rsph4a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rsph4a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa21274	NM_001107629.2 Latest version!	Rattus norvegicus radial spoke head component 4A (Rsph4a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ORa21274	XM_017588068.1 Latest version!	Rattus norvegicus radial spoke head 4 homolog A (Rsph4a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ORa21274	XM_003751961.4 Latest version!	Rattus norvegicus radial spoke head 4 homolog A (Rsph4a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rsph4a ( NM_001107629.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001101099.2 Rattus norvegicus radial spoke head component 4A (Rsph4a), mRNA.

Price & Availability↑

CloneID	ORa21274
Clone ID Related Accession (Same CDS sequence)	NM_001107629.2 , XM_017588068.1 , XM_003751961.4
Accession Version	NM_001107629.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2136bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565280000000
Organism	Rattus norvegicus(Norway rat)
Product	radial spoke head protein 4 homolog A
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07045038.1. On or before Aug 10, 2019 this sequence version replaced XM_003751961.4, XM_017588068.1, NM_001107629.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2689599, SAMEA2689601 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END##

ATGGAAAATT CTACCTCTCT GAAACAAGAA AAGGAAAATC AAGAGCCAGA GGGAGCAGAG 
AGATCATGGC AAGGAGAGTC CGAAGTTTCT CCCCCGGATC CTGCTCTCGC ATCACCTGAG 
TACCGGGAAG AGGAGCAAAG GTCAGACACC GAACCCTCAC CCAAAAGGAG CCCCTCTTGG 
GGCAGCCAGC CTAGAGCCAG CCTGTCTCTG GGACACCTCA CCGCTGGACT AGGAGTGTCA 
TCCTCCCCTC CCCCACCTCC TCTGGAGTTT CATTCCACGC CTCTCCAAAC TACAACTACT 
CAGGATCCTG TGACTGCATC ACACGCAGAG AGGGCCCCTA GTGGGATTAC TGACACCGGG 
ACGCCTTATT CTGATCCTTG GGAACCATCT TCTGCTGCAA GAAAATCAAC TTCTCACTAC 
ACAAGCCACA CAGAGGAAAG TGCCTTCCCG CTGTCACAGC CACCCCACCC TGACCTGGGT 
GGGAGAAGGG ATGCCGCCTG GAACAAGCCC AAGTACAAAG GGCTGAGATT TGACCTTTTA 
CAAGAGGAAG ACTCAAACAG TAACTGTGAT CCAGACCAGC CTGAGGTTGG TGCCAGCGAA 
GCAGCCCCCA GTATGCTTGA GGTGGCCGTC CAGAATGCAA AAGCCTACCT GCTGAGTACC 
AGCAGCAAGT CTGGCTTAAA CCTATATGAT CATCTTTCTA AAGTGTTGAC CAAGATACTA 
GATGAGCGTC CTGTGGATGC TGTTGACGTC GTTGAGAATA TAAGCCAGGA TGTGAAAATG 
TCACATTTTG ATAAAAAATT AGACACCCTC CACAATGAAA ACGAGATGCT TCCAGCGTAT 
GAGCTAGCAG AGACACAGAA GGCCCTTTTC CTGCAGGGAC ATTTGGAAGG AGCTGATTCA 
GAATTGGAGG AGGAAATTGC AGAAAGCTCT CTGCCCAATG TGATGGAATC GGCTTATTAT 
TTCGAGCAAG CTGGAGTCGG TTTGGGAACA GATGAGACTT ATCGAATATT TCTTGCCCTC 
AAGCAACTTA CTGACACACA CCCAATCCAA AGATGCCGCT TCTGGGGCAA GATCTTGGGG 
CTGGAAATGA ACTACATTGT TGCCGAGGTG GAATTTCGTG ACGGCGAAGA CGAAGAGGAG 
GTGGAAGAGG AAGGGATAGC TGAAGAGAGG GACAATGGAG GAAGTGAAGC CGGTGACAGC 
GAGGAAGATG AATTACCGAA GCCCTTGTAC AAGGCCCCAC AGGCTATACC TAAGGAAGAG 
AGTAGAACTG GTGCCAACAA GTATGTCTAT TTCGTTTGCA GTGAGCCTGG GAGGCCGTGG 
GTAAGGTTGC CATCAGTTAC ACCCGCGCAA ATTGTCACTG CAAGAAAAAT CAAGAAGTTT 
TTCACGGGGC GACTAGACGC AGCCATCGTA AGCTACCCAC CCTTCCCAGG GAATGAGAGC 
AATTACTTAA GAGCACAAAT TGCCCGCATT TCGGCAGGGA CGCACGTCAG CCCTCTGGGA 
TTCTATCAGT TTGGTGAAGA GGAAGGAGAG GAAGAGGAGG TGGAAGGTGG TCGGGACAGC 
TATGAGGAAA ACTCTGACTT TGAAGGCATC CAAGTGATTG ACCTGGTGGA GTCTCTCTCC 
AACTGGGTTC ATCACGCGCA GTACATTCTT CCTCAGGGTC GCTGTAATTG GTTCAACCCT 
ACACAAAAAG ATGAAGAGGA AGAGGAAGAA GATGAAGAAA AGGGGGAAGA GCCTGACTAC 
GTAGAGCAGG AAGTGGGGCC TCCTCTTTTG ACACCAATCT CTGAAGACCT AGAGATTCAG 
AATATACCCT CTTGGACGAC ACGGCTGTCC TCAAATCTCG TTCCACAGTA CGCCATCGCT 
GTCCTTCGAT CCAATCTTTG GCCTGGAGCA TATGCCTTTT CCAACGGCAA GAAGTTTGAA 
AACTTGTACA TAGGCTGGGG TCATAAGTAC AGTGTGGAGA ATTACACACC TCCAGGCCCA 
CCACCAGTTT ATCAAGAATA TCCCAGTGGA CCAGAAATTG CAGAAATGGA TGATCCCAGT 
GTGGAGGAGG AACAGGCTTT CAGAATTACA CAAGAGGCAG CTGCACTTTC AGCCGAGGAG 
AATGAAGAAA CTGAAGACGA GGACAATGAC GACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001101099.2
CDS	140..2275
Translation	MENSTSLKQEKENQEPEGAERSWQGESEVSPPDPALASPEYREEEQRSDTEPSPKRSPSWGSQPRASLSLGHLTAGLGVSSSPPPPPLEFHSTPLQTTTTQDPVTASHAERAPSGITDTGTPYSDPWEPSSAARKSTSHYTSHTEESAFPLSQPPHPDLGGRRDAAWNKPKYKGLRFDLLQEEDSNSNCDPDQPEVGASEAAPSMLEVAVQNAKAYLLSTSSKSGLNLYDHLSKVLTKILDERPVDAVDVVENISQDVKMSHFDKKLDTLHNENEMLPAYELAETQKALFLQGHLEGADSELEEEIAESSLPNVMESAYYFEQAGVGLGTDETYRIFLALKQLTDTHPIQRCRFWGKILGLEMNYIVAEVEFRDGEDEEEVEEEGIAEERDNGGSEAGDSEEDELPKPLYKAPQAIPKEESRTGANKYVYFVCSEPGRPWVRLPSVTPAQIVTARKIKKFFTGRLDAAIVSYPPFPGNESNYLRAQIARISAGTHVSPLGFYQFGEEEGEEEEVEGGRDSYEENSDFEGIQVIDLVESLSNWVHHAQYILPQGRCNWFNPTQKDEEEEEEDEEKGEEPDYVEQEVGPPLLTPISEDLEIQNIPSWTTRLSSNLVPQYAIAVLRSNLWPGAYAFSNGKKFENLYIGWGHKYSVENYTPPGPPPVYQEYPSGPEIAEMDDPSVEEEQAFRITQEAAALSAEENEETEDEDNDD

Target ORF information:

RefSeq Version	NM_001107629.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus radial spoke head component 4A (Rsph4a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001107629.2

ORF Insert Sequence:

ATGGAAAATT CTACCTCTCT GAAACAAGAA AAGGAAAATC AAGAGCCAGA GGGAGCAGAG 
AGATCATGGC AAGGAGAGTC CGAAGTTTCT CCCCCGGATC CTGCTCTCGC ATCACCTGAG 
TACCGGGAAG AGGAGCAAAG GTCAGACACC GAACCCTCAC CCAAAAGGAG CCCCTCTTGG 
GGCAGCCAGC CTAGAGCCAG CCTGTCTCTG GGACACCTCA CCGCTGGACT AGGAGTGTCA 
TCCTCCCCTC CCCCACCTCC TCTGGAGTTT CATTCCACGC CTCTCCAAAC TACAACTACT 
CAGGATCCTG TGACTGCATC ACACGCAGAG AGGGCCCCTA GTGGGATTAC TGACACCGGG 
ACGCCTTATT CTGATCCTTG GGAACCATCT TCTGCTGCAA GAAAATCAAC TTCTCACTAC 
ACAAGCCACA CAGAGGAAAG TGCCTTCCCG CTGTCACAGC CACCCCACCC TGACCTGGGT 
GGGAGAAGGG ATGCCGCCTG GAACAAGCCC AAGTACAAAG GGCTGAGATT TGACCTTTTA 
CAAGAGGAAG ACTCAAACAG TAACTGTGAT CCAGACCAGC CTGAGGTTGG TGCCAGCGAA 
GCAGCCCCCA GTATGCTTGA GGTGGCCGTC CAGAATGCAA AAGCCTACCT GCTGAGTACC 
AGCAGCAAGT CTGGCTTAAA CCTATATGAT CATCTTTCTA AAGTGTTGAC CAAGATACTA 
GATGAGCGTC CTGTGGATGC TGTTGACGTC GTTGAGAATA TAAGCCAGGA TGTGAAAATG 
TCACATTTTG ATAAAAAATT AGACACCCTC CACAATGAAA ACGAGATGCT TCCAGCGTAT 
GAGCTAGCAG AGACACAGAA GGCCCTTTTC CTGCAGGGAC ATTTGGAAGG AGCTGATTCA 
GAATTGGAGG AGGAAATTGC AGAAAGCTCT CTGCCCAATG TGATGGAATC GGCTTATTAT 
TTCGAGCAAG CTGGAGTCGG TTTGGGAACA GATGAGACTT ATCGAATATT TCTTGCCCTC 
AAGCAACTTA CTGACACACA CCCAATCCAA AGATGCCGCT TCTGGGGCAA GATCTTGGGG 
CTGGAAATGA ACTACATTGT TGCCGAGGTG GAATTTCGTG ACGGCGAAGA CGAAGAGGAG 
GTGGAAGAGG AAGGGATAGC TGAAGAGAGG GACAATGGAG GAAGTGAAGC CGGTGACAGC 
GAGGAAGATG AATTACCGAA GCCCTTGTAC AAGGCCCCAC AGGCTATACC TAAGGAAGAG 
AGTAGAACTG GTGCCAACAA GTATGTCTAT TTCGTTTGCA GTGAGCCTGG GAGGCCGTGG 
GTAAGGTTGC CATCAGTTAC ACCCGCGCAA ATTGTCACTG CAAGAAAAAT CAAGAAGTTT 
TTCACGGGGC GACTAGACGC AGCCATCGTA AGCTACCCAC CCTTCCCAGG GAATGAGAGC 
AATTACTTAA GAGCACAAAT TGCCCGCATT TCGGCAGGGA CGCACGTCAG CCCTCTGGGA 
TTCTATCAGT TTGGTGAAGA GGAAGGAGAG GAAGAGGAGG TGGAAGGTGG TCGGGACAGC 
TATGAGGAAA ACTCTGACTT TGAAGGCATC CAAGTGATTG ACCTGGTGGA GTCTCTCTCC 
AACTGGGTTC ATCACGCGCA GTACATTCTT CCTCAGGGTC GCTGTAATTG GTTCAACCCT 
ACACAAAAAG ATGAAGAGGA AGAGGAAGAA GATGAAGAAA AGGGGGAAGA GCCTGACTAC 
GTAGAGCAGG AAGTGGGGCC TCCTCTTTTG ACACCAATCT CTGAAGACCT AGAGATTCAG 
AATATACCCT CTTGGACGAC ACGGCTGTCC TCAAATCTCG TTCCACAGTA CGCCATCGCT 
GTCCTTCGAT CCAATCTTTG GCCTGGAGCA TATGCCTTTT CCAACGGCAA GAAGTTTGAA 
AACTTGTACA TAGGCTGGGG TCATAAGTAC AGTGTGGAGA ATTACACACC TCCAGGCCCA 
CCACCAGTTT ATCAAGAATA TCCCAGTGGA CCAGAAATTG CAGAAATGGA TGATCCCAGT 
GTGGAGGAGG AACAGGCTTT CAGAATTACA CAAGAGGCAG CTGCACTTTC AGCCGAGGAG 
AATGAAGAAA CTGAAGACGA GGACAATGAC GACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rsph4a ( XM_017588068.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017443557.1 Rattus norvegicus radial spoke head 4 homolog A (Rsph4a), mRNA.

Price & Availability↑

CloneID	ORa21274
Clone ID Related Accession (Same CDS sequence)	NM_001107629.2 , XM_017588068.1 , XM_003751961.4
Accession Version	XM_017588068.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2136bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	radial spoke head protein 4 homolog A
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000088.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAATT CTACCTCTCT GAAACAAGAA AAGGAAAATC AAGAGCCAGA GGGAGCAGAG 
AGATCATGGC AAGGAGAGTC CGAAGTTTCT CCCCCGGATC CTGCTCTCGC ATCACCTGAG 
TACCGGGAAG AGGAGCAAAG GTCAGACACC GAACCCTCAC CCAAAAGGAG CCCCTCTTGG 
GGCAGCCAGC CTAGAGCCAG CCTGTCTCTG GGACACCTCA CCGCTGGACT AGGAGTGTCA 
TCCTCCCCTC CCCCACCTCC TCTGGAGTTT CATTCCACGC CTCTCCAAAC TACAACTACT 
CAGGATCCTG TGACTGCATC ACACGCAGAG AGGGCCCCTA GTGGGATTAC TGACACCGGG 
ACGCCTTATT CTGATCCTTG GGAACCATCT TCTGCTGCAA GAAAATCAAC TTCTCACTAC 
ACAAGCCACA CAGAGGAAAG TGCCTTCCCG CTGTCACAGC CACCCCACCC TGACCTGGGT 
GGGAGAAGGG ATGCCGCCTG GAACAAGCCC AAGTACAAAG GGCTGAGATT TGACCTTTTA 
CAAGAGGAAG ACTCAAACAG TAACTGTGAT CCAGACCAGC CTGAGGTTGG TGCCAGCGAA 
GCAGCCCCCA GTATGCTTGA GGTGGCCGTC CAGAATGCAA AAGCCTACCT GCTGAGTACC 
AGCAGCAAGT CTGGCTTAAA CCTATATGAT CATCTTTCTA AAGTGTTGAC CAAGATACTA 
GATGAGCGTC CTGTGGATGC TGTTGACGTC GTTGAGAATA TAAGCCAGGA TGTGAAAATG 
TCACATTTTG ATAAAAAATT AGACACCCTC CACAATGAAA ACGAGATGCT TCCAGCGTAT 
GAGCTAGCAG AGACACAGAA GGCCCTTTTC CTGCAGGGAC ATTTGGAAGG AGCTGATTCA 
GAATTGGAGG AGGAAATTGC AGAAAGCTCT CTGCCCAATG TGATGGAATC GGCTTATTAT 
TTCGAGCAAG CTGGAGTCGG TTTGGGAACA GATGAGACTT ATCGAATATT TCTTGCCCTC 
AAGCAACTTA CTGACACACA CCCAATCCAA AGATGCCGCT TCTGGGGCAA GATCTTGGGG 
CTGGAAATGA ACTACATTGT TGCCGAGGTG GAATTTCGTG ACGGCGAAGA CGAAGAGGAG 
GTGGAAGAGG AAGGGATAGC TGAAGAGAGG GACAATGGAG GAAGTGAAGC CGGTGACAGC 
GAGGAAGATG AATTACCGAA GCCCTTGTAC AAGGCCCCAC AGGCTATACC TAAGGAAGAG 
AGTAGAACTG GTGCCAACAA GTATGTCTAT TTCGTTTGCA GTGAGCCTGG GAGGCCGTGG 
GTAAGGTTGC CATCAGTTAC ACCCGCGCAA ATTGTCACTG CAAGAAAAAT CAAGAAGTTT 
TTCACGGGGC GACTAGACGC AGCCATCGTA AGCTACCCAC CCTTCCCAGG GAATGAGAGC 
AATTACTTAA GAGCACAAAT TGCCCGCATT TCGGCAGGGA CGCACGTCAG CCCTCTGGGA 
TTCTATCAGT TTGGTGAAGA GGAAGGAGAG GAAGAGGAGG TGGAAGGTGG TCGGGACAGC 
TATGAGGAAA ACTCTGACTT TGAAGGCATC CAAGTGATTG ACCTGGTGGA GTCTCTCTCC 
AACTGGGTTC ATCACGCGCA GTACATTCTT CCTCAGGGTC GCTGTAATTG GTTCAACCCT 
ACACAAAAAG ATGAAGAGGA AGAGGAAGAA GATGAAGAAA AGGGGGAAGA GCCTGACTAC 
GTAGAGCAGG AAGTGGGGCC TCCTCTTTTG ACACCAATCT CTGAAGACCT AGAGATTCAG 
AATATACCCT CTTGGACGAC ACGGCTGTCC TCAAATCTCG TTCCACAGTA CGCCATCGCT 
GTCCTTCGAT CCAATCTTTG GCCTGGAGCA TATGCCTTTT CCAACGGCAA GAAGTTTGAA 
AACTTGTACA TAGGCTGGGG TCATAAGTAC AGTGTGGAGA ATTACACACC TCCAGGCCCA 
CCACCAGTTT ATCAAGAATA TCCCAGTGGA CCAGAAATTG CAGAAATGGA TGATCCCAGT 
GTGGAGGAGG AACAGGCTTT CAGAATTACA CAAGAGGCAG CTGCACTTTC AGCCGAGGAG 
AATGAAGAAA CTGAAGACGA GGACAATGAC GACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017443557.1
CDS	140..2275
Translation	MENSTSLKQEKENQEPEGAERSWQGESEVSPPDPALASPEYREEEQRSDTEPSPKRSPSWGSQPRASLSLGHLTAGLGVSSSPPPPPLEFHSTPLQTTTTQDPVTASHAERAPSGITDTGTPYSDPWEPSSAARKSTSHYTSHTEESAFPLSQPPHPDLGGRRDAAWNKPKYKGLRFDLLQEEDSNSNCDPDQPEVGASEAAPSMLEVAVQNAKAYLLSTSSKSGLNLYDHLSKVLTKILDERPVDAVDVVENISQDVKMSHFDKKLDTLHNENEMLPAYELAETQKALFLQGHLEGADSELEEEIAESSLPNVMESAYYFEQAGVGLGTDETYRIFLALKQLTDTHPIQRCRFWGKILGLEMNYIVAEVEFRDGEDEEEVEEEGIAEERDNGGSEAGDSEEDELPKPLYKAPQAIPKEESRTGANKYVYFVCSEPGRPWVRLPSVTPAQIVTARKIKKFFTGRLDAAIVSYPPFPGNESNYLRAQIARISAGTHVSPLGFYQFGEEEGEEEEVEGGRDSYEENSDFEGIQVIDLVESLSNWVHHAQYILPQGRCNWFNPTQKDEEEEEEDEEKGEEPDYVEQEVGPPLLTPISEDLEIQNIPSWTTRLSSNLVPQYAIAVLRSNLWPGAYAFSNGKKFENLYIGWGHKYSVENYTPPGPPPVYQEYPSGPEIAEMDDPSVEEEQAFRITQEAAALSAEENEETEDEDNDD

Target ORF information:

RefSeq Version	XM_017588068.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus radial spoke head 4 homolog A (Rsph4a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017588068.1

ORF Insert Sequence:

ATGGAAAATT CTACCTCTCT GAAACAAGAA AAGGAAAATC AAGAGCCAGA GGGAGCAGAG 
AGATCATGGC AAGGAGAGTC CGAAGTTTCT CCCCCGGATC CTGCTCTCGC ATCACCTGAG 
TACCGGGAAG AGGAGCAAAG GTCAGACACC GAACCCTCAC CCAAAAGGAG CCCCTCTTGG 
GGCAGCCAGC CTAGAGCCAG CCTGTCTCTG GGACACCTCA CCGCTGGACT AGGAGTGTCA 
TCCTCCCCTC CCCCACCTCC TCTGGAGTTT CATTCCACGC CTCTCCAAAC TACAACTACT 
CAGGATCCTG TGACTGCATC ACACGCAGAG AGGGCCCCTA GTGGGATTAC TGACACCGGG 
ACGCCTTATT CTGATCCTTG GGAACCATCT TCTGCTGCAA GAAAATCAAC TTCTCACTAC 
ACAAGCCACA CAGAGGAAAG TGCCTTCCCG CTGTCACAGC CACCCCACCC TGACCTGGGT 
GGGAGAAGGG ATGCCGCCTG GAACAAGCCC AAGTACAAAG GGCTGAGATT TGACCTTTTA 
CAAGAGGAAG ACTCAAACAG TAACTGTGAT CCAGACCAGC CTGAGGTTGG TGCCAGCGAA 
GCAGCCCCCA GTATGCTTGA GGTGGCCGTC CAGAATGCAA AAGCCTACCT GCTGAGTACC 
AGCAGCAAGT CTGGCTTAAA CCTATATGAT CATCTTTCTA AAGTGTTGAC CAAGATACTA 
GATGAGCGTC CTGTGGATGC TGTTGACGTC GTTGAGAATA TAAGCCAGGA TGTGAAAATG 
TCACATTTTG ATAAAAAATT AGACACCCTC CACAATGAAA ACGAGATGCT TCCAGCGTAT 
GAGCTAGCAG AGACACAGAA GGCCCTTTTC CTGCAGGGAC ATTTGGAAGG AGCTGATTCA 
GAATTGGAGG AGGAAATTGC AGAAAGCTCT CTGCCCAATG TGATGGAATC GGCTTATTAT 
TTCGAGCAAG CTGGAGTCGG TTTGGGAACA GATGAGACTT ATCGAATATT TCTTGCCCTC 
AAGCAACTTA CTGACACACA CCCAATCCAA AGATGCCGCT TCTGGGGCAA GATCTTGGGG 
CTGGAAATGA ACTACATTGT TGCCGAGGTG GAATTTCGTG ACGGCGAAGA CGAAGAGGAG 
GTGGAAGAGG AAGGGATAGC TGAAGAGAGG GACAATGGAG GAAGTGAAGC CGGTGACAGC 
GAGGAAGATG AATTACCGAA GCCCTTGTAC AAGGCCCCAC AGGCTATACC TAAGGAAGAG 
AGTAGAACTG GTGCCAACAA GTATGTCTAT TTCGTTTGCA GTGAGCCTGG GAGGCCGTGG 
GTAAGGTTGC CATCAGTTAC ACCCGCGCAA ATTGTCACTG CAAGAAAAAT CAAGAAGTTT 
TTCACGGGGC GACTAGACGC AGCCATCGTA AGCTACCCAC CCTTCCCAGG GAATGAGAGC 
AATTACTTAA GAGCACAAAT TGCCCGCATT TCGGCAGGGA CGCACGTCAG CCCTCTGGGA 
TTCTATCAGT TTGGTGAAGA GGAAGGAGAG GAAGAGGAGG TGGAAGGTGG TCGGGACAGC 
TATGAGGAAA ACTCTGACTT TGAAGGCATC CAAGTGATTG ACCTGGTGGA GTCTCTCTCC 
AACTGGGTTC ATCACGCGCA GTACATTCTT CCTCAGGGTC GCTGTAATTG GTTCAACCCT 
ACACAAAAAG ATGAAGAGGA AGAGGAAGAA GATGAAGAAA AGGGGGAAGA GCCTGACTAC 
GTAGAGCAGG AAGTGGGGCC TCCTCTTTTG ACACCAATCT CTGAAGACCT AGAGATTCAG 
AATATACCCT CTTGGACGAC ACGGCTGTCC TCAAATCTCG TTCCACAGTA CGCCATCGCT 
GTCCTTCGAT CCAATCTTTG GCCTGGAGCA TATGCCTTTT CCAACGGCAA GAAGTTTGAA 
AACTTGTACA TAGGCTGGGG TCATAAGTAC AGTGTGGAGA ATTACACACC TCCAGGCCCA 
CCACCAGTTT ATCAAGAATA TCCCAGTGGA CCAGAAATTG CAGAAATGGA TGATCCCAGT 
GTGGAGGAGG AACAGGCTTT CAGAATTACA CAAGAGGCAG CTGCACTTTC AGCCGAGGAG 
AATGAAGAAA CTGAAGACGA GGACAATGAC GACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rsph4a ( XM_003751961.4 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_003752009.1 Rattus norvegicus radial spoke head 4 homolog A (Rsph4a), mRNA.

Price & Availability↑

CloneID	ORa21274
Clone ID Related Accession (Same CDS sequence)	NM_001107629.2 , XM_017588068.1 , XM_003751961.4
Accession Version	XM_003751961.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2136bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	radial spoke head protein 4 homolog A
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005119.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_003751961.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAATT CTACCTCTCT GAAACAAGAA AAGGAAAATC AAGAGCCAGA GGGAGCAGAG 
AGATCATGGC AAGGAGAGTC CGAAGTTTCT CCCCCGGATC CTGCTCTCGC ATCACCTGAG 
TACCGGGAAG AGGAGCAAAG GTCAGACACC GAACCCTCAC CCAAAAGGAG CCCCTCTTGG 
GGCAGCCAGC CTAGAGCCAG CCTGTCTCTG GGACACCTCA CCGCTGGACT AGGAGTGTCA 
TCCTCCCCTC CCCCACCTCC TCTGGAGTTT CATTCCACGC CTCTCCAAAC TACAACTACT 
CAGGATCCTG TGACTGCATC ACACGCAGAG AGGGCCCCTA GTGGGATTAC TGACACCGGG 
ACGCCTTATT CTGATCCTTG GGAACCATCT TCTGCTGCAA GAAAATCAAC TTCTCACTAC 
ACAAGCCACA CAGAGGAAAG TGCCTTCCCG CTGTCACAGC CACCCCACCC TGACCTGGGT 
GGGAGAAGGG ATGCCGCCTG GAACAAGCCC AAGTACAAAG GGCTGAGATT TGACCTTTTA 
CAAGAGGAAG ACTCAAACAG TAACTGTGAT CCAGACCAGC CTGAGGTTGG TGCCAGCGAA 
GCAGCCCCCA GTATGCTTGA GGTGGCCGTC CAGAATGCAA AAGCCTACCT GCTGAGTACC 
AGCAGCAAGT CTGGCTTAAA CCTATATGAT CATCTTTCTA AAGTGTTGAC CAAGATACTA 
GATGAGCGTC CTGTGGATGC TGTTGACGTC GTTGAGAATA TAAGCCAGGA TGTGAAAATG 
TCACATTTTG ATAAAAAATT AGACACCCTC CACAATGAAA ACGAGATGCT TCCAGCGTAT 
GAGCTAGCAG AGACACAGAA GGCCCTTTTC CTGCAGGGAC ATTTGGAAGG AGCTGATTCA 
GAATTGGAGG AGGAAATTGC AGAAAGCTCT CTGCCCAATG TGATGGAATC GGCTTATTAT 
TTCGAGCAAG CTGGAGTCGG TTTGGGAACA GATGAGACTT ATCGAATATT TCTTGCCCTC 
AAGCAACTTA CTGACACACA CCCAATCCAA AGATGCCGCT TCTGGGGCAA GATCTTGGGG 
CTGGAAATGA ACTACATTGT TGCCGAGGTG GAATTTCGTG ACGGCGAAGA CGAAGAGGAG 
GTGGAAGAGG AAGGGATAGC TGAAGAGAGG GACAATGGAG GAAGTGAAGC CGGTGACAGC 
GAGGAAGATG AATTACCGAA GCCCTTGTAC AAGGCCCCAC AGGCTATACC TAAGGAAGAG 
AGTAGAACTG GTGCCAACAA GTATGTCTAT TTCGTTTGCA GTGAGCCTGG GAGGCCGTGG 
GTAAGGTTGC CATCAGTTAC ACCCGCGCAA ATTGTCACTG CAAGAAAAAT CAAGAAGTTT 
TTCACGGGGC GACTAGACGC AGCCATCGTA AGCTACCCAC CCTTCCCAGG GAATGAGAGC 
AATTACTTAA GAGCACAAAT TGCCCGCATT TCGGCAGGGA CGCACGTCAG CCCTCTGGGA 
TTCTATCAGT TTGGTGAAGA GGAAGGAGAG GAAGAGGAGG TGGAAGGTGG TCGGGACAGC 
TATGAGGAAA ACTCTGACTT TGAAGGCATC CAAGTGATTG ACCTGGTGGA GTCTCTCTCC 
AACTGGGTTC ATCACGCGCA GTACATTCTT CCTCAGGGTC GCTGTAATTG GTTCAACCCT 
ACACAAAAAG ATGAAGAGGA AGAGGAAGAA GATGAAGAAA AGGGGGAAGA GCCTGACTAC 
GTAGAGCAGG AAGTGGGGCC TCCTCTTTTG ACACCAATCT CTGAAGACCT AGAGATTCAG 
AATATACCCT CTTGGACGAC ACGGCTGTCC TCAAATCTCG TTCCACAGTA CGCCATCGCT 
GTCCTTCGAT CCAATCTTTG GCCTGGAGCA TATGCCTTTT CCAACGGCAA GAAGTTTGAA 
AACTTGTACA TAGGCTGGGG TCATAAGTAC AGTGTGGAGA ATTACACACC TCCAGGCCCA 
CCACCAGTTT ATCAAGAATA TCCCAGTGGA CCAGAAATTG CAGAAATGGA TGATCCCAGT 
GTGGAGGAGG AACAGGCTTT CAGAATTACA CAAGAGGCAG CTGCACTTTC AGCCGAGGAG 
AATGAAGAAA CTGAAGACGA GGACAATGAC GACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003752009.1
CDS	140..2275
Translation	MENSTSLKQEKENQEPEGAERSWQGESEVSPPDPALASPEYREEEQRSDTEPSPKRSPSWGSQPRASLSLGHLTAGLGVSSSPPPPPLEFHSTPLQTTTTQDPVTASHAERAPSGITDTGTPYSDPWEPSSAARKSTSHYTSHTEESAFPLSQPPHPDLGGRRDAAWNKPKYKGLRFDLLQEEDSNSNCDPDQPEVGASEAAPSMLEVAVQNAKAYLLSTSSKSGLNLYDHLSKVLTKILDERPVDAVDVVENISQDVKMSHFDKKLDTLHNENEMLPAYELAETQKALFLQGHLEGADSELEEEIAESSLPNVMESAYYFEQAGVGLGTDETYRIFLALKQLTDTHPIQRCRFWGKILGLEMNYIVAEVEFRDGEDEEEVEEEGIAEERDNGGSEAGDSEEDELPKPLYKAPQAIPKEESRTGANKYVYFVCSEPGRPWVRLPSVTPAQIVTARKIKKFFTGRLDAAIVSYPPFPGNESNYLRAQIARISAGTHVSPLGFYQFGEEEGEEEEVEGGRDSYEENSDFEGIQVIDLVESLSNWVHHAQYILPQGRCNWFNPTQKDEEEEEEDEEKGEEPDYVEQEVGPPLLTPISEDLEIQNIPSWTTRLSSNLVPQYAIAVLRSNLWPGAYAFSNGKKFENLYIGWGHKYSVENYTPPGPPPVYQEYPSGPEIAEMDDPSVEEEQAFRITQEAAALSAEENEETEDEDNDD

Target ORF information:

RefSeq Version	XM_003751961.4
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus radial spoke head 4 homolog A (Rsph4a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003751961.4

ORF Insert Sequence:

ATGGAAAATT CTACCTCTCT GAAACAAGAA AAGGAAAATC AAGAGCCAGA GGGAGCAGAG 
AGATCATGGC AAGGAGAGTC CGAAGTTTCT CCCCCGGATC CTGCTCTCGC ATCACCTGAG 
TACCGGGAAG AGGAGCAAAG GTCAGACACC GAACCCTCAC CCAAAAGGAG CCCCTCTTGG 
GGCAGCCAGC CTAGAGCCAG CCTGTCTCTG GGACACCTCA CCGCTGGACT AGGAGTGTCA 
TCCTCCCCTC CCCCACCTCC TCTGGAGTTT CATTCCACGC CTCTCCAAAC TACAACTACT 
CAGGATCCTG TGACTGCATC ACACGCAGAG AGGGCCCCTA GTGGGATTAC TGACACCGGG 
ACGCCTTATT CTGATCCTTG GGAACCATCT TCTGCTGCAA GAAAATCAAC TTCTCACTAC 
ACAAGCCACA CAGAGGAAAG TGCCTTCCCG CTGTCACAGC CACCCCACCC TGACCTGGGT 
GGGAGAAGGG ATGCCGCCTG GAACAAGCCC AAGTACAAAG GGCTGAGATT TGACCTTTTA 
CAAGAGGAAG ACTCAAACAG TAACTGTGAT CCAGACCAGC CTGAGGTTGG TGCCAGCGAA 
GCAGCCCCCA GTATGCTTGA GGTGGCCGTC CAGAATGCAA AAGCCTACCT GCTGAGTACC 
AGCAGCAAGT CTGGCTTAAA CCTATATGAT CATCTTTCTA AAGTGTTGAC CAAGATACTA 
GATGAGCGTC CTGTGGATGC TGTTGACGTC GTTGAGAATA TAAGCCAGGA TGTGAAAATG 
TCACATTTTG ATAAAAAATT AGACACCCTC CACAATGAAA ACGAGATGCT TCCAGCGTAT 
GAGCTAGCAG AGACACAGAA GGCCCTTTTC CTGCAGGGAC ATTTGGAAGG AGCTGATTCA 
GAATTGGAGG AGGAAATTGC AGAAAGCTCT CTGCCCAATG TGATGGAATC GGCTTATTAT 
TTCGAGCAAG CTGGAGTCGG TTTGGGAACA GATGAGACTT ATCGAATATT TCTTGCCCTC 
AAGCAACTTA CTGACACACA CCCAATCCAA AGATGCCGCT TCTGGGGCAA GATCTTGGGG 
CTGGAAATGA ACTACATTGT TGCCGAGGTG GAATTTCGTG ACGGCGAAGA CGAAGAGGAG 
GTGGAAGAGG AAGGGATAGC TGAAGAGAGG GACAATGGAG GAAGTGAAGC CGGTGACAGC 
GAGGAAGATG AATTACCGAA GCCCTTGTAC AAGGCCCCAC AGGCTATACC TAAGGAAGAG 
AGTAGAACTG GTGCCAACAA GTATGTCTAT TTCGTTTGCA GTGAGCCTGG GAGGCCGTGG 
GTAAGGTTGC CATCAGTTAC ACCCGCGCAA ATTGTCACTG CAAGAAAAAT CAAGAAGTTT 
TTCACGGGGC GACTAGACGC AGCCATCGTA AGCTACCCAC CCTTCCCAGG GAATGAGAGC 
AATTACTTAA GAGCACAAAT TGCCCGCATT TCGGCAGGGA CGCACGTCAG CCCTCTGGGA 
TTCTATCAGT TTGGTGAAGA GGAAGGAGAG GAAGAGGAGG TGGAAGGTGG TCGGGACAGC 
TATGAGGAAA ACTCTGACTT TGAAGGCATC CAAGTGATTG ACCTGGTGGA GTCTCTCTCC 
AACTGGGTTC ATCACGCGCA GTACATTCTT CCTCAGGGTC GCTGTAATTG GTTCAACCCT 
ACACAAAAAG ATGAAGAGGA AGAGGAAGAA GATGAAGAAA AGGGGGAAGA GCCTGACTAC 
GTAGAGCAGG AAGTGGGGCC TCCTCTTTTG ACACCAATCT CTGAAGACCT AGAGATTCAG 
AATATACCCT CTTGGACGAC ACGGCTGTCC TCAAATCTCG TTCCACAGTA CGCCATCGCT 
GTCCTTCGAT CCAATCTTTG GCCTGGAGCA TATGCCTTTT CCAACGGCAA GAAGTTTGAA 
AACTTGTACA TAGGCTGGGG TCATAAGTAC AGTGTGGAGA ATTACACACC TCCAGGCCCA 
CCACCAGTTT ATCAAGAATA TCCCAGTGGA CCAGAAATTG CAGAAATGGA TGATCCCAGT 
GTGGAGGAGG AACAGGCTTT CAGAATTACA CAAGAGGCAG CTGCACTTTC AGCCGAGGAG 
AATGAAGAAA CTGAAGACGA GGACAATGAC GACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mutations in CCDC39 and CCDC40 are the major cause of primary ciliary dyskinesia with axonemal disorganization and absent inner dynein arms.
Human mutation34(3)462-72(2013 Mar)
Antony D,Becker-Heck A,Zariwala MA,Schmidts M,Onoufriadis A,Forouhan M,Wilson R,Taylor-Cox T,Dewar A,Jackson C,Goggin P,Loges NT,Olbrich H,Jaspers M,Jorissen M,Leigh MW,Wolf WE,Daniels ML,Noone PG,Ferkol TW,Sagel SD,Rosenfeld M,Rutman A,Dixit A,O'Callaghan C,Lucas JS,Hogg C,Scambler PJ,Emes RD,,Chung EM,Shoemark A,Knowles MR,Omran H,Mitchison HM

Mutations in radial spoke head protein genes RSPH9 and RSPH4A cause primary ciliary dyskinesia with central-microtubular-pair abnormalities.
American journal of human genetics84(2)197-209(2009 Feb)
Castleman VH,Romio L,Chodhari R,Hirst RA,de Castro SC,Parker KA,Ybot-Gonzalez P,Emes RD,Wilson SW,Wallis C,Johnson CA,Herrera RJ,Rutman A,Dixon M,Shoemark A,Bush A,Hogg C,Gardiner RM,Reish O,Greene ND,O'Callaghan C,Purton S,Chung EM,Mitchison HM

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Rsph4a cDNA ORF clone, Rattus norvegicus(Norway rat)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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